Ush1c cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ush1c
Entrez Gene ID	308596
Full Name	USH1 protein network component harmonin
General protein information	Preferred Names USH1 protein network component harmonin Names harmonin Usher syndrome 1C homolog harmonin a1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22
Summary	forms a protein complex that includes myosin VIIa, and cadherin 2; may play a role in organization of synaptic junctions at photoreceptor synapses [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006229221.3	XP_006229283.1	harmonin isoform X1
XM_006229222.3	XP_006229284.1	harmonin isoform X2
NM_212521.2	NP_997686.2	harmonin

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	LOC696911	XP_001085522.2
Rattus norvegicus (Norway rat)	Ush1c	NP_997686.2

Gene Ontology
Bibliography

Related articles in PubMed

ANKS4B Is Essential for Intermicrovillar Adhesion Complex Formation.
Crawley SW, Weck ML, Grega-Larson NE, Shifrin DA Jr, Tyska MJ
Developmental cell36(2)190-200(2016 Jan)

Class III myosins shape the auditory hair bundles by limiting microvilli and stereocilia growth.
Lelli A, Michel V, Boutet de Monvel J, Cortese M, Bosch-Grau M, Aghaie A, Perfettini I, Dupont T, Avan P, El-Amraoui A, Petit C
The Journal of cell biology212(2)231-44(2016 Jan)

Intestinal brush border assembly driven by protocadherin-based intermicrovillar adhesion.
Crawley SW, Shifrin DA Jr, Grega-Larson NE, McConnell RE, Benesh AE, Mao S, Zheng Y, Zheng QY, Nam KT, Millis BA, Kachar B, Tyska MJ
Cell157(2)433-446(2014 Apr)

The giant spectrin βV couples the molecular motors to phototransduction and Usher syndrome type I proteins along their trafficking route.
Papal S, Cortese M, Legendre K, Sorusch N, Dragavon J, Sahly I, Shorte S, Wolfrum U, Petit C, El-Amraoui A
Human molecular genetics22(18)3773-88(2013 Sep)

Rescue of hearing and vestibular function by antisense oligonucleotides in a mouse model of human deafness.
Lentz JJ, Jodelka FM, Hinrich AJ, McCaffrey KE, Farris HE, Spalitta MJ, Bazan NG, Duelli DM, Rigo F, Hastings ML
Nature medicine19(3)345-50(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ush1c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ush1c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05784	NM_212521.2 Latest version!	Rattus norvegicus USH1 protein network component harmonin (Ush1c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa05784	XM_006229221.3 Latest version!	Rattus norvegicus USH1 protein network component harmonin (Ush1c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa22806	XM_006229222.3 Latest version!	Rattus norvegicus USH1 protein network component harmonin (Ush1c), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ush1c ( NM_212521.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_997686.2 Rattus norvegicus USH1 protein network component harmonin (Ush1c), mRNA.

Price & Availability↑

CloneID	ORa05784
Clone ID Related Accession (Same CDS sequence)	XM_006229221.3 , NM_212521.2
Accession Version	NM_212521.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527350400000
Organism	Rattus norvegicus(Norway rat)
Product	harmonin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC098641.1. On Jul 13, 2005 this sequence version replaced NM_212521.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098641.1, AY588414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296 [ECO:0000348] ##Evidence-Data-END##

ATGGACCGGA AGGTGGCCCG AGAATTCCGA CACAAGGTGG ATTTTCTCAT TGAAAATGAC 
GCGGAGAAGG ACTATCTTTA TGACGTGCTG CGGATGTATC ACCAGACCAT GGACGTGGCT 
GTGCTCGTGG GAGACCTGAA GCTGGTCATC AATGAGCCCA ACCGCCTGCC ACTCTTCGAC 
GCCATCCGGC CCCTGATCCC ACTGAAGCAC CAGGTGGAGT ATGACCAGCT GACACCCCGA 
CGCTCCAGGA AACTGAAGGA GGTGCGTTTG GACCGTCTGC ACCCAGAGGG CCTTGGCCTC 
AGCGTGCGTG GAGGCCTGGA ATTTGGCTGT GGGCTCTTCA TCTCCCATCT CATCAAAGGT 
GGCCAGGCAG ACAGCGTTGG GCTGCAGGTA GGGGACGAGA TTGTCCGGAT CAACGGCTAT 
TCCATCTCCT CCTGCACCCA CGAGGAAGTC ATCAACCTGA TCCGCACCAA GAAGACTGTG 
TCCATCAAAG TGAGACACAT CGGCCTGATC CCTGTGAAGA GCTCCCCCGA GGAGCCCCTC 
AAGTGGCAGT ATGTGGATCA GTTCGTGTCG GAATCTGGAG GTGTTCGAGG TGGCTTGAGC 
TCACCTGGCA ATCGAACAAC CAAGGAGAAG AAGGTGTTTA TCAGTCTAGT AGGTTCTCGG 
GGCCTGGGCT GCAGCATTTC CAGTGGCCCC ATCCAGAAGC CTGGCATCTT CGTCAGCAAC 
GTGAAACCTG GTTCCCTGTC TGCAGAGGTG GGGTTAGAGA CAGGAGACCA GATTGTGGAA 
GTCAATGGCA TCGACTTCAC CAACCTGGAC CACAAGGAGG CCGTGAATGT CCTGAAGAGC 
AGCCGCAGCC TGACCATCTC CATTGTTGCT GGAGCTGGCC GGGAGCTGTT TATGACAGAC 
CGGGAACGGC TGGAAGAGGC ACGGCAGCAT GAGCTGCAGC GGCAAGAGCT CCTCATGCAG 
AAGCGGCTGG CCATGGAGTC CAACAAGATC CTCCAGGAGC AGCAGGAGAT GGAGCGTCAG 
AGGAGAAAGG AAATCGCCCA GAAGGCCGCT GAGGAGAATG AGAGATACCG GAAGGAGATG 
GAACAGATCT CGGAGGAGGA AGAGAAGTTT AAGAAGCAGT GGGAAGAAGA CTGGGGTTCA 
AAGGAACAGC TCATTCTGCC TAAAACCATC ACCGCAGAGG TGCACCCGGT GCCGCTTCGC 
AAGCCCAAGT ATGATCAGGG AGTGGAGCCT GCTGACCACT TGGATGGAAG CACGAAGAAG 
CAGAGACAGC AGGATTTCCG AAAATATGAA GAAGGCTTTG ATCCTTACTC CATGTTCTCG 
CCAGAACAAA TTGCAGGGAA GGATGTCCGG CTGCTGAGAA TCAAGAAGGA GGGGTCTTTA 
GACTTGGCCT TGGAGGGGGG AGTGGACTCC CCCGTGGGGA AGGTGGTCGT CTCTGCTGTT 
TATGAAGGAG GAGCTGCAGA GCGGCATGGT GGCGTTGTGA AAGGGGATGA GATCATGGCC 
ATCAACGGCA AGATCGTGAC GGACTACACC CTGGCTGAGG CTGAGGCTGC CCTGCAGAAG 
GCCTGGAATC AGGGCGGGGA CTGGATAGAC CTTGTGGTTG CCGTCTGTCC CCCCAAGGAA 
TACGATGATG AACTAACCTT CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997686.2
CDS	119..1765
Translation	MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPNRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPEEPLKWQYVDQFVSESGGVRGGLSSPGNRTTKEKKVFISLVGSRGLGCSISSGPIQKPGIFVSNVKPGSLSAEVGLETGDQIVEVNGIDFTNLDHKEAVNVLKSSRSLTISIVAGAGRELFMTDRERLEEARQHELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQISEEEEKFKKQWEEDWGSKEQLILPKTITAEVHPVPLRKPKYDQGVEPADHLDGSTKKQRQQDFRKYEEGFDPYSMFSPEQIAGKDVRLLRIKKEGSLDLALEGGVDSPVGKVVVSAVYEGGAAERHGGVVKGDEIMAINGKIVTDYTLAEAEAALQKAWNQGGDWIDLVVAVCPPKEYDDELTFF

Target ORF information:

RefSeq Version	NM_212521.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus USH1 protein network component harmonin (Ush1c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212521.2

ORF Insert Sequence:

ATGGACCGGA AGGTGGCCCG AGAATTCCGA CACAAGGTGG ATTTTCTCAT TGAAAATGAC 
GCGGAGAAGG ACTATCTTTA TGACGTGCTG CGGATGTATC ACCAGACCAT GGACGTGGCT 
GTGCTCGTGG GAGACCTGAA GCTGGTCATC AATGAGCCCA ACCGCCTGCC ACTCTTCGAC 
GCCATCCGGC CCCTGATCCC ACTGAAGCAC CAGGTGGAGT ATGACCAGCT GACACCCCGA 
CGCTCCAGGA AACTGAAGGA GGTGCGTTTG GACCGTCTGC ACCCAGAGGG CCTTGGCCTC 
AGCGTGCGTG GAGGCCTGGA ATTTGGCTGT GGGCTCTTCA TCTCCCATCT CATCAAAGGT 
GGCCAGGCAG ACAGCGTTGG GCTGCAGGTA GGGGACGAGA TTGTCCGGAT CAACGGCTAT 
TCCATCTCCT CCTGCACCCA CGAGGAAGTC ATCAACCTGA TCCGCACCAA GAAGACTGTG 
TCCATCAAAG TGAGACACAT CGGCCTGATC CCTGTGAAGA GCTCCCCCGA GGAGCCCCTC 
AAGTGGCAGT ATGTGGATCA GTTCGTGTCG GAATCTGGAG GTGTTCGAGG TGGCTTGAGC 
TCACCTGGCA ATCGAACAAC CAAGGAGAAG AAGGTGTTTA TCAGTCTAGT AGGTTCTCGG 
GGCCTGGGCT GCAGCATTTC CAGTGGCCCC ATCCAGAAGC CTGGCATCTT CGTCAGCAAC 
GTGAAACCTG GTTCCCTGTC TGCAGAGGTG GGGTTAGAGA CAGGAGACCA GATTGTGGAA 
GTCAATGGCA TCGACTTCAC CAACCTGGAC CACAAGGAGG CCGTGAATGT CCTGAAGAGC 
AGCCGCAGCC TGACCATCTC CATTGTTGCT GGAGCTGGCC GGGAGCTGTT TATGACAGAC 
CGGGAACGGC TGGAAGAGGC ACGGCAGCAT GAGCTGCAGC GGCAAGAGCT CCTCATGCAG 
AAGCGGCTGG CCATGGAGTC CAACAAGATC CTCCAGGAGC AGCAGGAGAT GGAGCGTCAG 
AGGAGAAAGG AAATCGCCCA GAAGGCCGCT GAGGAGAATG AGAGATACCG GAAGGAGATG 
GAACAGATCT CGGAGGAGGA AGAGAAGTTT AAGAAGCAGT GGGAAGAAGA CTGGGGTTCA 
AAGGAACAGC TCATTCTGCC TAAAACCATC ACCGCAGAGG TGCACCCGGT GCCGCTTCGC 
AAGCCCAAGT ATGATCAGGG AGTGGAGCCT GCTGACCACT TGGATGGAAG CACGAAGAAG 
CAGAGACAGC AGGATTTCCG AAAATATGAA GAAGGCTTTG ATCCTTACTC CATGTTCTCG 
CCAGAACAAA TTGCAGGGAA GGATGTCCGG CTGCTGAGAA TCAAGAAGGA GGGGTCTTTA 
GACTTGGCCT TGGAGGGGGG AGTGGACTCC CCCGTGGGGA AGGTGGTCGT CTCTGCTGTT 
TATGAAGGAG GAGCTGCAGA GCGGCATGGT GGCGTTGTGA AAGGGGATGA GATCATGGCC 
ATCAACGGCA AGATCGTGAC GGACTACACC CTGGCTGAGG CTGAGGCTGC CCTGCAGAAG 
GCCTGGAATC AGGGCGGGGA CTGGATAGAC CTTGTGGTTG CCGTCTGTCC CCCCAAGGAA 
TACGATGATG AACTAACCTT CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ush1c ( XM_006229221.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229283.1 Rattus norvegicus USH1 protein network component harmonin (Ush1c), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa05784
Clone ID Related Accession (Same CDS sequence)	XM_006229221.3 , NM_212521.2
Accession Version	XM_006229221.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	harmonin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229221.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGGA AGGTGGCCCG AGAATTCCGA CACAAGGTGG ATTTTCTCAT TGAAAATGAC 
GCGGAGAAGG ACTATCTTTA TGACGTGCTG CGGATGTATC ACCAGACCAT GGACGTGGCT 
GTGCTCGTGG GAGACCTGAA GCTGGTCATC AATGAGCCCA ACCGCCTGCC ACTCTTCGAC 
GCCATCCGGC CCCTGATCCC ACTGAAGCAC CAGGTGGAGT ATGACCAGCT GACACCCCGA 
CGCTCCAGGA AACTGAAGGA GGTGCGTTTG GACCGTCTGC ACCCAGAGGG CCTTGGCCTC 
AGCGTGCGTG GAGGCCTGGA ATTTGGCTGT GGGCTCTTCA TCTCCCATCT CATCAAAGGT 
GGCCAGGCAG ACAGCGTTGG GCTGCAGGTA GGGGACGAGA TTGTCCGGAT CAACGGCTAT 
TCCATCTCCT CCTGCACCCA CGAGGAAGTC ATCAACCTGA TCCGCACCAA GAAGACTGTG 
TCCATCAAAG TGAGACACAT CGGCCTGATC CCTGTGAAGA GCTCCCCCGA GGAGCCCCTC 
AAGTGGCAGT ATGTGGATCA GTTCGTGTCG GAATCTGGAG GTGTTCGAGG TGGCTTGAGC 
TCACCTGGCA ATCGAACAAC CAAGGAGAAG AAGGTGTTTA TCAGTCTAGT AGGTTCTCGG 
GGCCTGGGCT GCAGCATTTC CAGTGGCCCC ATCCAGAAGC CTGGCATCTT CGTCAGCAAC 
GTGAAACCTG GTTCCCTGTC TGCAGAGGTG GGGTTAGAGA CAGGAGACCA GATTGTGGAA 
GTCAATGGCA TCGACTTCAC CAACCTGGAC CACAAGGAGG CCGTGAATGT CCTGAAGAGC 
AGCCGCAGCC TGACCATCTC CATTGTTGCT GGAGCTGGCC GGGAGCTGTT TATGACAGAC 
CGGGAACGGC TGGAAGAGGC ACGGCAGCAT GAGCTGCAGC GGCAAGAGCT CCTCATGCAG 
AAGCGGCTGG CCATGGAGTC CAACAAGATC CTCCAGGAGC AGCAGGAGAT GGAGCGTCAG 
AGGAGAAAGG AAATCGCCCA GAAGGCCGCT GAGGAGAATG AGAGATACCG GAAGGAGATG 
GAACAGATCT CGGAGGAGGA AGAGAAGTTT AAGAAGCAGT GGGAAGAAGA CTGGGGTTCA 
AAGGAACAGC TCATTCTGCC TAAAACCATC ACCGCAGAGG TGCACCCGGT GCCGCTTCGC 
AAGCCCAAGT ATGATCAGGG AGTGGAGCCT GCTGACCACT TGGATGGAAG CACGAAGAAG 
CAGAGACAGC AGGATTTCCG AAAATATGAA GAAGGCTTTG ATCCTTACTC CATGTTCTCG 
CCAGAACAAA TTGCAGGGAA GGATGTCCGG CTGCTGAGAA TCAAGAAGGA GGGGTCTTTA 
GACTTGGCCT TGGAGGGGGG AGTGGACTCC CCCGTGGGGA AGGTGGTCGT CTCTGCTGTT 
TATGAAGGAG GAGCTGCAGA GCGGCATGGT GGCGTTGTGA AAGGGGATGA GATCATGGCC 
ATCAACGGCA AGATCGTGAC GGACTACACC CTGGCTGAGG CTGAGGCTGC CCTGCAGAAG 
GCCTGGAATC AGGGCGGGGA CTGGATAGAC CTTGTGGTTG CCGTCTGTCC CCCCAAGGAA 
TACGATGATG AACTAACCTT CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229283.1
CDS	452..2098
Translation	MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPNRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPEEPLKWQYVDQFVSESGGVRGGLSSPGNRTTKEKKVFISLVGSRGLGCSISSGPIQKPGIFVSNVKPGSLSAEVGLETGDQIVEVNGIDFTNLDHKEAVNVLKSSRSLTISIVAGAGRELFMTDRERLEEARQHELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQISEEEEKFKKQWEEDWGSKEQLILPKTITAEVHPVPLRKPKYDQGVEPADHLDGSTKKQRQQDFRKYEEGFDPYSMFSPEQIAGKDVRLLRIKKEGSLDLALEGGVDSPVGKVVVSAVYEGGAAERHGGVVKGDEIMAINGKIVTDYTLAEAEAALQKAWNQGGDWIDLVVAVCPPKEYDDELTFF

Target ORF information:

RefSeq Version	XM_006229221.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus USH1 protein network component harmonin (Ush1c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229221.3

ORF Insert Sequence:

ATGGACCGGA AGGTGGCCCG AGAATTCCGA CACAAGGTGG ATTTTCTCAT TGAAAATGAC 
GCGGAGAAGG ACTATCTTTA TGACGTGCTG CGGATGTATC ACCAGACCAT GGACGTGGCT 
GTGCTCGTGG GAGACCTGAA GCTGGTCATC AATGAGCCCA ACCGCCTGCC ACTCTTCGAC 
GCCATCCGGC CCCTGATCCC ACTGAAGCAC CAGGTGGAGT ATGACCAGCT GACACCCCGA 
CGCTCCAGGA AACTGAAGGA GGTGCGTTTG GACCGTCTGC ACCCAGAGGG CCTTGGCCTC 
AGCGTGCGTG GAGGCCTGGA ATTTGGCTGT GGGCTCTTCA TCTCCCATCT CATCAAAGGT 
GGCCAGGCAG ACAGCGTTGG GCTGCAGGTA GGGGACGAGA TTGTCCGGAT CAACGGCTAT 
TCCATCTCCT CCTGCACCCA CGAGGAAGTC ATCAACCTGA TCCGCACCAA GAAGACTGTG 
TCCATCAAAG TGAGACACAT CGGCCTGATC CCTGTGAAGA GCTCCCCCGA GGAGCCCCTC 
AAGTGGCAGT ATGTGGATCA GTTCGTGTCG GAATCTGGAG GTGTTCGAGG TGGCTTGAGC 
TCACCTGGCA ATCGAACAAC CAAGGAGAAG AAGGTGTTTA TCAGTCTAGT AGGTTCTCGG 
GGCCTGGGCT GCAGCATTTC CAGTGGCCCC ATCCAGAAGC CTGGCATCTT CGTCAGCAAC 
GTGAAACCTG GTTCCCTGTC TGCAGAGGTG GGGTTAGAGA CAGGAGACCA GATTGTGGAA 
GTCAATGGCA TCGACTTCAC CAACCTGGAC CACAAGGAGG CCGTGAATGT CCTGAAGAGC 
AGCCGCAGCC TGACCATCTC CATTGTTGCT GGAGCTGGCC GGGAGCTGTT TATGACAGAC 
CGGGAACGGC TGGAAGAGGC ACGGCAGCAT GAGCTGCAGC GGCAAGAGCT CCTCATGCAG 
AAGCGGCTGG CCATGGAGTC CAACAAGATC CTCCAGGAGC AGCAGGAGAT GGAGCGTCAG 
AGGAGAAAGG AAATCGCCCA GAAGGCCGCT GAGGAGAATG AGAGATACCG GAAGGAGATG 
GAACAGATCT CGGAGGAGGA AGAGAAGTTT AAGAAGCAGT GGGAAGAAGA CTGGGGTTCA 
AAGGAACAGC TCATTCTGCC TAAAACCATC ACCGCAGAGG TGCACCCGGT GCCGCTTCGC 
AAGCCCAAGT ATGATCAGGG AGTGGAGCCT GCTGACCACT TGGATGGAAG CACGAAGAAG 
CAGAGACAGC AGGATTTCCG AAAATATGAA GAAGGCTTTG ATCCTTACTC CATGTTCTCG 
CCAGAACAAA TTGCAGGGAA GGATGTCCGG CTGCTGAGAA TCAAGAAGGA GGGGTCTTTA 
GACTTGGCCT TGGAGGGGGG AGTGGACTCC CCCGTGGGGA AGGTGGTCGT CTCTGCTGTT 
TATGAAGGAG GAGCTGCAGA GCGGCATGGT GGCGTTGTGA AAGGGGATGA GATCATGGCC 
ATCAACGGCA AGATCGTGAC GGACTACACC CTGGCTGAGG CTGAGGCTGC CCTGCAGAAG 
GCCTGGAATC AGGGCGGGGA CTGGATAGAC CTTGTGGTTG CCGTCTGTCC CCCCAAGGAA 
TACGATGATG AACTAACCTT CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ush1c ( XM_006229222.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229284.1 Rattus norvegicus USH1 protein network component harmonin (Ush1c), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa22806
Clone ID Related Accession (Same CDS sequence)	XM_006229222.3
Accession Version	XM_006229222.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	harmonin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229222.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGGA AGGTGGCCCG AGAATTCCGA CACAAGGTGG ATTTTCTCAT TGAAAATGAC 
GCGGAGAAGG ACTATCTTTA TGACGTGCTG CGGATGTATC ACCAGACCAT GGACGTGGCT 
GTGCTCGTGG GAGACCTGAA GCTGGTCATC AATGAGCCCA ACCGCCTGCC ACTCTTCGAC 
GCCATCCGGC CCCTGATCCC ACTGAAGCAC CAGGTGGAGT ATGACCAGCT GACACCCCGA 
CGCTCCAGGA AACTGAAGGA GGTGCGTTTG GACCGTCTGC ACCCAGAGGG CCTTGGCCTC 
AGCGTGCGTG GAGGCCTGGA ATTTGGCTGT GGGCTCTTCA TCTCCCATCT CATCAAAGGT 
GGCCAGGCAG ACAGCGTTGG GCTGCAGGTA GGGGACGAGA TTGTCCGGAT CAACGGCTAT 
TCCATCTCCT CCTGCACCCA CGAGGAAGTC ATCAACCTGA TCCGCACCAA GAAGACTGTG 
TCCATCAAAG TGAGACACAT CGGCCTGATC CCTGTGAAGA GCTCCCCCGA GGAGCCCCTC 
AAGTGGCAGT ATGTGGATCA GTTCGTGTCG GAATCTGGAG GTGTTCGAGG TGGCTTGAGC 
TCACCTGGCA ATCGAACAAC CAAGGAGAAG AAGGTGTTTA TCAGTCTAGT AGGTTCTCGG 
GGCCTGGGCT GCAGCATTTC CAGTGGCCCC ATCCAGAAGC CTGGCATCTT CGTCAGCAAC 
GTGAAACCTG GTTCCCTGTC TGCAGAGGTG GGGTTAGAGA CAGGAGACCA GATTGTGGAA 
GTCAATGGCA TCGACTTCAC CAACCTGGAC CACAAGGAGG CCGTGAATGT CCTGAAGAGC 
AGCCGCAGCC TGACCATCTC CATTGTTGCT GGAGCTGGCC GGGAGCTGTT TATGACAGAC 
CGGGAACGGC TGGAAGAGGC ACGGCAGCAT GAGCTGCAGC GGCAAGAGCT CCTCATGCAG 
AAGCGGCTGG CCATGGAGTC CAACAAGATC CTCCAGGAGC AGCAGGAGAT GGAGCGTCAG 
AGGAGAAAGG AAATCGCCCA GAAGGCCGCT GAGGAGAATG AGAGATACCG GAAGGAGATG 
GAACAGATCT CGGAGGAGGA AGAGAAGTTT AAGAAGCAGT GGGAAGAAGA CTGGGGTTCA 
AAGGAACAGC TCATTCTGCC TAAAACCATC ACCGCAGAGG TGCACCCGGT GCCGCTTCGC 
AAGCCCAAGT CCTTTGGGTG GTTTTACCGT TACGATGGCA AATTTCCAAC CATCCGGAAG 
AAAGCCAAAG AGAAGAAGAA AGCCAAGTAC GACAGCCTGC AGGACCTGAG GAAAAACAAG 
AAGGAACTCG AGTTTGAGCA AAAGCTCTAC AAAGAGAAGG AAGAGATGCT GGAGAAGGAG 
AAGCAGCTGA AGATCAACAG GCTAGCCCAG GAGGTGTCTG AGACTGAACG GGAAGATCTT 
GAGGAATCGG AAAAGACTCA GTATTGGGTG GAGAGGCTCT GTCAGACTCG CCTCCAGCAG 
ATTTCCTCTG CTGAGAATGA GATTACTGAG ATGACCACAG GGCCCCCACC TCCCCCACCT 
TCTGTGTCTC CTCTGGTCCC ACCCTTGAGA CGATTCGCTG GTGGAATCCA CCTCCATACC 
ACTGACTTGG ATGACATTCC CCTGGACATG TTCTACTATC CCCCCAAGAC TCCCTCAGCA 
CTGCCTGTGA TGCCCCACCC TCCACCCAGC AACCCACCTT CCAAGGTCCC CGCCCCTCCT 
GTACTTCCGT CCTCAGACCA CATGAGCAGC TCATCCTCAC CATGGGTGCA ACACACCCCA 
CCCCCAATTC CCATCCCTCC CCCTCCGTCC ATTCCCACCC AAGACCTCAC TCCTACCCGC 
CCACTGCCCT CAGCACTGGA AGAGGTCCTG GGCAACCACC CCTTCCGCAC TGGGGACCCA 
GGCCATCCAG CAACCGACTG GGAGGCAAAC ACCCACAGTG GGAAGCCCAC CAGCTCCCCC 
ACCCCTGAGC GGAGCTTCCC GCCCGCCCCT AAGACATTTT GCCCAAGTCC ACAGCCTCCA 
CGAGGCCCTG GCGTGTCCAC CATCTCCAAA CCCGTCATGG TCCATCAGGA ACACAACTTT 
GTCTACAGGC CAGCTGTGAA GTCTGAGGTC CTGCCGCAGG AGATGCTGAA GAGGATGGTG 
GTATATCAGA CAGCATTCAG ACAAGATTTC CGAAAATATG AAGAAGGCTT TGATCCTTAC 
TCCATGTTCT CGCCAGAACA AATTGCAGGG AAGGATGTCC GGCTGCTGAG AATCAAGAAG 
GAGGGGTCTT TAGACTTGGC CTTGGAGGGG GGAGTGGACT CCCCCGTGGG GAAGGTGGTC 
GTCTCTGCTG TTTATGAAGG AGGAGCTGCA GAGCGGCATG GTGGCGTTGT GAAAGGGGAT 
GAGATCATGG CCATCAACGG CAAGATCGTG ACGGACTACA CCCTGGCTGA GGCTGAGGCT 
GCCCTGCAGA AGGCCTGGAA TCAGGGCGGG GACTGGATAG ACCTTGTGGT TGCCGTCTGT 
CCCCCCAAGG AATACGATGA TGAACTCTCC TCTCTTCCCT CCTCTGCAGC CGAAAGCCCC 
CAGCCGGTCC GAAAGCAGCT TGAAGCCTAT GAGCCCGTGT GTAGACACGG GTTTTTCCTG 
CAGCTGGAGC CCACGAACCT TCTTTTAAAG TCCAGAGACA GAAGCCAGCC AGACCCATCC 
TGGAAGCTGG CAAGCTCGGC ACCCAGCCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229284.1
CDS	502..3234
Translation	MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPNRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPEEPLKWQYVDQFVSESGGVRGGLSSPGNRTTKEKKVFISLVGSRGLGCSISSGPIQKPGIFVSNVKPGSLSAEVGLETGDQIVEVNGIDFTNLDHKEAVNVLKSSRSLTISIVAGAGRELFMTDRERLEEARQHELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQISEEEEKFKKQWEEDWGSKEQLILPKTITAEVHPVPLRKPKSFGWFYRYDGKFPTIRKKAKEKKKAKYDSLQDLRKNKKELEFEQKLYKEKEEMLEKEKQLKINRLAQEVSETEREDLEESEKTQYWVERLCQTRLQQISSAENEITEMTTGPPPPPPSVSPLVPPLRRFAGGIHLHTTDLDDIPLDMFYYPPKTPSALPVMPHPPPSNPPSKVPAPPVLPSSDHMSSSSSPWVQHTPPPIPIPPPPSIPTQDLTPTRPLPSALEEVLGNHPFRTGDPGHPATDWEANTHSGKPTSSPTPERSFPPAPKTFCPSPQPPRGPGVSTISKPVMVHQEHNFVYRPAVKSEVLPQEMLKRMVVYQTAFRQDFRKYEEGFDPYSMFSPEQIAGKDVRLLRIKKEGSLDLALEGGVDSPVGKVVVSAVYEGGAAERHGGVVKGDEIMAINGKIVTDYTLAEAEAALQKAWNQGGDWIDLVVAVCPPKEYDDELSSLPSSAAESPQPVRKQLEAYEPVCRHGFFLQLEPTNLLLKSRDRSQPDPSWKLASSAPSP

Target ORF information:

RefSeq Version	XM_006229222.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus USH1 protein network component harmonin (Ush1c), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229222.3

ORF Insert Sequence:

ATGGACCGGA AGGTGGCCCG AGAATTCCGA CACAAGGTGG ATTTTCTCAT TGAAAATGAC 
GCGGAGAAGG ACTATCTTTA TGACGTGCTG CGGATGTATC ACCAGACCAT GGACGTGGCT 
GTGCTCGTGG GAGACCTGAA GCTGGTCATC AATGAGCCCA ACCGCCTGCC ACTCTTCGAC 
GCCATCCGGC CCCTGATCCC ACTGAAGCAC CAGGTGGAGT ATGACCAGCT GACACCCCGA 
CGCTCCAGGA AACTGAAGGA GGTGCGTTTG GACCGTCTGC ACCCAGAGGG CCTTGGCCTC 
AGCGTGCGTG GAGGCCTGGA ATTTGGCTGT GGGCTCTTCA TCTCCCATCT CATCAAAGGT 
GGCCAGGCAG ACAGCGTTGG GCTGCAGGTA GGGGACGAGA TTGTCCGGAT CAACGGCTAT 
TCCATCTCCT CCTGCACCCA CGAGGAAGTC ATCAACCTGA TCCGCACCAA GAAGACTGTG 
TCCATCAAAG TGAGACACAT CGGCCTGATC CCTGTGAAGA GCTCCCCCGA GGAGCCCCTC 
AAGTGGCAGT ATGTGGATCA GTTCGTGTCG GAATCTGGAG GTGTTCGAGG TGGCTTGAGC 
TCACCTGGCA ATCGAACAAC CAAGGAGAAG AAGGTGTTTA TCAGTCTAGT AGGTTCTCGG 
GGCCTGGGCT GCAGCATTTC CAGTGGCCCC ATCCAGAAGC CTGGCATCTT CGTCAGCAAC 
GTGAAACCTG GTTCCCTGTC TGCAGAGGTG GGGTTAGAGA CAGGAGACCA GATTGTGGAA 
GTCAATGGCA TCGACTTCAC CAACCTGGAC CACAAGGAGG CCGTGAATGT CCTGAAGAGC 
AGCCGCAGCC TGACCATCTC CATTGTTGCT GGAGCTGGCC GGGAGCTGTT TATGACAGAC 
CGGGAACGGC TGGAAGAGGC ACGGCAGCAT GAGCTGCAGC GGCAAGAGCT CCTCATGCAG 
AAGCGGCTGG CCATGGAGTC CAACAAGATC CTCCAGGAGC AGCAGGAGAT GGAGCGTCAG 
AGGAGAAAGG AAATCGCCCA GAAGGCCGCT GAGGAGAATG AGAGATACCG GAAGGAGATG 
GAACAGATCT CGGAGGAGGA AGAGAAGTTT AAGAAGCAGT GGGAAGAAGA CTGGGGTTCA 
AAGGAACAGC TCATTCTGCC TAAAACCATC ACCGCAGAGG TGCACCCGGT GCCGCTTCGC 
AAGCCCAAGT CCTTTGGGTG GTTTTACCGT TACGATGGCA AATTTCCAAC CATCCGGAAG 
AAAGCCAAAG AGAAGAAGAA AGCCAAGTAC GACAGCCTGC AGGACCTGAG GAAAAACAAG 
AAGGAACTCG AGTTTGAGCA AAAGCTCTAC AAAGAGAAGG AAGAGATGCT GGAGAAGGAG 
AAGCAGCTGA AGATCAACAG GCTAGCCCAG GAGGTGTCTG AGACTGAACG GGAAGATCTT 
GAGGAATCGG AAAAGACTCA GTATTGGGTG GAGAGGCTCT GTCAGACTCG CCTCCAGCAG 
ATTTCCTCTG CTGAGAATGA GATTACTGAG ATGACCACAG GGCCCCCACC TCCCCCACCT 
TCTGTGTCTC CTCTGGTCCC ACCCTTGAGA CGATTCGCTG GTGGAATCCA CCTCCATACC 
ACTGACTTGG ATGACATTCC CCTGGACATG TTCTACTATC CCCCCAAGAC TCCCTCAGCA 
CTGCCTGTGA TGCCCCACCC TCCACCCAGC AACCCACCTT CCAAGGTCCC CGCCCCTCCT 
GTACTTCCGT CCTCAGACCA CATGAGCAGC TCATCCTCAC CATGGGTGCA ACACACCCCA 
CCCCCAATTC CCATCCCTCC CCCTCCGTCC ATTCCCACCC AAGACCTCAC TCCTACCCGC 
CCACTGCCCT CAGCACTGGA AGAGGTCCTG GGCAACCACC CCTTCCGCAC TGGGGACCCA 
GGCCATCCAG CAACCGACTG GGAGGCAAAC ACCCACAGTG GGAAGCCCAC CAGCTCCCCC 
ACCCCTGAGC GGAGCTTCCC GCCCGCCCCT AAGACATTTT GCCCAAGTCC ACAGCCTCCA 
CGAGGCCCTG GCGTGTCCAC CATCTCCAAA CCCGTCATGG TCCATCAGGA ACACAACTTT 
GTCTACAGGC CAGCTGTGAA GTCTGAGGTC CTGCCGCAGG AGATGCTGAA GAGGATGGTG 
GTATATCAGA CAGCATTCAG ACAAGATTTC CGAAAATATG AAGAAGGCTT TGATCCTTAC 
TCCATGTTCT CGCCAGAACA AATTGCAGGG AAGGATGTCC GGCTGCTGAG AATCAAGAAG 
GAGGGGTCTT TAGACTTGGC CTTGGAGGGG GGAGTGGACT CCCCCGTGGG GAAGGTGGTC 
GTCTCTGCTG TTTATGAAGG AGGAGCTGCA GAGCGGCATG GTGGCGTTGT GAAAGGGGAT 
GAGATCATGG CCATCAACGG CAAGATCGTG ACGGACTACA CCCTGGCTGA GGCTGAGGCT 
GCCCTGCAGA AGGCCTGGAA TCAGGGCGGG GACTGGATAG ACCTTGTGGT TGCCGTCTGT 
CCCCCCAAGG AATACGATGA TGAACTCTCC TCTCTTCCCT CCTCTGCAGC CGAAAGCCCC 
CAGCCGGTCC GAAAGCAGCT TGAAGCCTAT GAGCCCGTGT GTAGACACGG GTTTTTCCTG 
CAGCTGGAGC CCACGAACCT TCTTTTAAAG TCCAGAGACA GAAGCCAGCC AGACCCATCC 
TGGAAGCTGG CAAGCTCGGC ACCCAGCCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ANKS4B Is Essential for Intermicrovillar Adhesion Complex Formation.
Developmental cell36(2)190-200(2016 Jan)
Crawley SW,Weck ML,Grega-Larson NE,Shifrin DA Jr,Tyska MJ

Class III myosins shape the auditory hair bundles by limiting microvilli and stereocilia growth.
The Journal of cell biology212(2)231-44(2016 Jan)
Lelli A,Michel V,Boutet de Monvel J,Cortese M,Bosch-Grau M,Aghaie A,Perfettini I,Dupont T,Avan P,El-Amraoui A,Petit C

Intestinal brush border assembly driven by protocadherin-based intermicrovillar adhesion.
Cell157(2)433-446(2014 Apr)
Crawley SW,Shifrin DA Jr,Grega-Larson NE,McConnell RE,Benesh AE,Mao S,Zheng Y,Zheng QY,Nam KT,Millis BA,Kachar B,Tyska MJ

The giant spectrin βV couples the molecular motors to phototransduction and Usher syndrome type I proteins along their trafficking route.
Human molecular genetics22(18)3773-88(2013 Sep)
Papal S,Cortese M,Legendre K,Sorusch N,Dragavon J,Sahly I,Shorte S,Wolfrum U,Petit C,El-Amraoui A

Rescue of hearing and vestibular function by antisense oligonucleotides in a mouse model of human deafness.
Nature medicine19(3)345-50(2013 Mar)
Lentz JJ,Jodelka FM,Hinrich AJ,McCaffrey KE,Farris HE,Spalitta MJ,Bazan NG,Duelli DM,Rigo F,Hastings ML

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Ush1c cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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