Eif5b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif5b
Entrez Gene ID	308306
Full Name	eukaryotic translation initiation factor 5B
General protein information	Preferred Names eukaryotic translation initiation factor 5B Names eukaryotic translation initiation factor 5B annexin V-binding protein ABP-7 eIF-5B translation initiation factor IF-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q21
Summary	translation initiation factor; binds annexin V in a Ca2+ - and phospholipid-dependent manner [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001110141.1	NP_001103611.1	eukaryotic translation initiation factor 5B

Pathways from BioSystems

Homologies

Homo sapiens (human)	EIF5B	NP_056988.3
Rattus norvegicus (Norway rat)	Eif5b	NP_001103611.1
Bos taurus (cattle)	EIF5B	XP_003586655.1
Gallus gallus (chicken)	EIF5B	NP_001033585.1
Pan troglodytes (chimpanzee)	EIF5B	XP_001159759.1
Canis lupus familiaris (dog)	EIF5B	XP_005626079.1
Macaca mulatta (Rhesus monkey)	EIF5B	XP_001103720.2
Xenopus tropicalis (tropical clawed frog)	eif5b	XP_004911863.1
Mus musculus (house mouse)	Eif5b	NP_938045.2
Danio rerio (zebrafish)	eif5b	XP_001923573.4

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanhäusser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm.
Bohnsack MT, Regener K, Schwappach B, Saffrich R, Paraskeva E, Hartmann E, Görlich D
The EMBO journal21(22)6205-15(2002 Nov)

Cloning and characterization of hIF2, a human homologue of bacterial translation initiation factor 2, and its interaction with HIV-1 matrix.
Wilson SA, Sieiro-Vazquez C, Edwards NJ, Iourin O, Byles ED, Kotsopoulou E, Adamson CS, Kingsman SM, Kingsman AJ, Martin-Rendon E
The Biochemical journal342 ( Pt 1)97-103(1999 Aug)

Molecular cloning and characterization of annexin V-binding proteins with highly hydrophilic peptide structure.
Ohsawa K, Imai Y, Ito D, Kohsaka S
Journal of neurochemistry67(1)89-97(1996 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Eif5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00768	NM_001110141.1 Latest version!	Rattus norvegicus eukaryotic translation initiation factor 5B (Eif5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif5b ( NM_001110141.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001103611.1 Rattus norvegicus eukaryotic translation initiation factor 5B (Eif5b), mRNA.

Price & Availability↑

CloneID	ORa00768
Clone ID Related Accession (Same CDS sequence)	NM_001110141.1
Accession Version	NM_001110141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic translation initiation factor 5B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07067426.1 and AABR07067427.1. On or before Oct 26, 2007 this sequence version replaced XM_001057637.1, XM_001057591.1, XM_218162.4, XM_001055509.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC166424.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGA AACAGAAAAA CAAGAGCGAA GACAGTACCA AGGATGATAC TGACTTGGGC 
GCCTTGGCTG CGGAAATAGA AGGAGCTGGT GCTGCCAAGG AGCAGGAGCC TCAGAAGGGA 
AAGGGGAAGA AGAAAAAGGA GAAAAAGAAG CAAGACTTTG ATGAGAACGA CATTCTGAGA 
GAGCTGGAGG AGTTGTCTCT GGAAGCCCAA GGCATCGGAG CTGACAGAGA TGCCGCTACA 
GTCAAGCCTA CAGAAAACAA TGAAGAAGAA TCTGCCTCAA AACAAGATAA AAAGAAGAAA 
GGACAAAAAG GCAAGAAAAC AAGTTTTGAT GAGAATGATA GTGAAGAATT GGAAGATAAA 
GATTCAAAAT CAAAAAAGCC TGCCAGACCT AACTCAGAGG TGCTCCTCTC TGGGAGTGAG 
GATGCAGATG ATCCTAATAA ACTTTCTAAA AAAGGTAAAA AAGCCCAGAA ATCAACTAAA 
AAACGGGATG GGTCGGAAGA GGATGAAGAC AATAGCAAAA GAAGTAAAGA ACGTTCGAGA 
GTAAACTCCT CAGGTGAAAG TGGTGGTGAG TCTGATGAAT TTTTGCAGTC CAGAAAAGGA 
CAGAAAAAAA ATCAGAAGAA CAAGTCAGTT CCTACCATAG ACAGTGGGAA TGAGGACGAT 
GACTCTTCCT TCAAAATTAA GACGGTGGCC CAGAAGAAGG CAGAAAAGAA AGAGCGTGAG 
AGGAAAAAGA GAGAAGAGGA GAAAGCAAAG TTGCGAAAGG TGAAAGAGAA AGAAGAGCTA 
GAGAAGGGCA GGAAGGAGCA GAGTAAGCAG AGGGAGCCTC AGAAGAGACC GGACGAGGAG 
GTGTTGGTGC TCAGAGGGAC TCCTGATGCA GGTGCTGCCT CAGAAGAGAA AGGAGACATT 
GCTGCAACTC TGGAAGATGA CAATGAAGGA GACAAAAAGA AAAAAGATAA GAAGAAAAAG 
AAAACAGAAA AAGATGATAA AGAAAAAGAG AAGAAGAAAG GCCCAAGCAA ATCCACTGTT 
AAAGCTATCC AGGAAGCTTT GGCTAAGCTC AGAGAGGAGG AGGAGAGACA GAAGAGAGAG 
GAGGAGGAAA GGATTAAGCG GCTTGAAGAA CTAGAAGCCA AACGAAAAGA AGAGGAACGA 
TTAGAACAAG AAAAAAGAGA AAGGAAAAAG CAAAAAGAAA AAGAAAGAAA AGAACGGTTA 
AAAAAAGAAG GAAAACTGTT AACTAAGTCC CAGAGAGAAG CCAGAGCCAG AGCTGAAGTG 
ACCCTGAGAC ACCTCCAAGC GCAGGGTGTT GAAGTGCCAT CAAAAGACTC GTTGCCAAAG 
AAGAGGCCAG TGTATGAAGA TAAGAAGAAG AAGAAAACAC CACAGCAGCT GGAGAGTAAA 
GAAGCACTTG AGACTGTGGA AGTCAGTGCC CCTGTGGAAG TTGTGGACCA AGGAGTACCA 
GAAAAGGAGG AGACACCGCC TTCTGTTGAC GCAGAGGAAG ACGAAGAAAC TGAAGATGCT 
GGGCTCGATG ACTGGGAAGC TATGGCCAGT GATGAGGAGA GAGAAAAAGA AGGAAATATG 
ATTCACATAG AAGTAGAAGA AAACCCTGAG GAAGAGGAGG AGGAAGAAGA GGATGAAGAT 
GAAGAAGACA GTGAAGATGA GGAAGACGAA GGGGACAGTG AGGGCAGCGA TGGGGACGAG 
GAGGACTACA AGCTATCGGA CGAGAAGGAC TTGGGGAAAG CTGGAGACAC GAAGCCCAAC 
AAAGACGCTA GCTCAGACTC CGAGTATGAC TCTGATGACG ATCGGACTAA AGAAGAGCGG 
GCGTACGACA AAGCAAAACG GAGAATTGAG AAACGACGAC TTGAACATGG TAAAAATGTA 
AATACAGAAA AGCTAAGAGC CCCTATCATC TGTGTGCTTG GACATGTAGA CACAGGGAAG 
ACAAAAATTC TAGATAAGCT CCGCCACACT CATGTACAAG ATGGTGAAGC AGGTGGCATC 
ACACAGCAGA TTGGTGCCAC AAATGTTCCT CTCGAAGCTA TTAATGAACA AACTAAAATG 
ATTAAAAATT TTGACAGAGA GAATGTCCGC ATTCCAGGGA TGCTGATTAT TGACACTCCT 
GGACATGAGT CTTTCAGTAA CCTGAGAAAC AGAGGAAGCT CTCTCTGTGA TATTGCCATT 
TTAGTTGTTG ACATTATGCA TGGTTTAGAG CCCCAGACAA TTGAATCAAT CAACATTCTC 
AAATCCAAAA AATGTCCCTT TATTGTTGCA CTTAATAAGA TTGACAGGTT ATATGATTGG 
AAGAAGAGTC CTGATTCGGA TGTGGCTGTT ACTTTAAAAA AGCAGAAGAA GAATACAAAG 
GATGAGTTTG AGGAGCGTGC AAAGGCCATT ATTGTAGAAT TTGCACAACA GGGTTTAAAT 
GCTGCTTTGT TTTATGAGAA TAAAGATCCC CGCACTTTTG TGTCCCTGGT ACCGACCTCT 
GCACACACTG GTGATGGCAT GGGAAGCCTC ATCTACCTTC TTGTGGAATT AACTCAAACT 
ATGTTGAGCA AGAGGCTTGC TCACTGTGAG GAGCTGAGAG CACAAGTCAT GGAGGTTAAA 
GCTCTCCCAG GAATGGGCAC CACAATAGAT GTCATATTGA TTAATGGACG TTTGAAGGAA 
GGGGACACAA TCATTGTTCC TGGAGTAGAA GGGCCCATTG TCACTCAGAT TCGAGGCCTC 
CTTTTACCTC CTCCTATGAA GGAATTACGC GTGAAGAACC AATATGAAAA GCATAAAGAA 
GTGGAAGCAG CCCAGGGAGT GAAGATTCTT GGAAAAGACC TTGAGAAAAC GCTGGCTGGT 
TTACCTCTCC TAGTGGCCTA TAAGGACGAC GAAATCCCAG TTCTCAAAGA TGAATTGATC 
CATGAGTTAA AGCAGACGCT GAATGCTATC AAATTGGAAG AAAAAGGAGT CTATGTTCAG 
GCATCTACAC TGGGCTCTTT GGAAGCTCTT CTTGAATTTC TGAAAACATC AGAGGTGCCA 
TATGCGGGAA TTAACATTGG CCCAGTCCAC AAGAAGGATG TTATGAAGGC TTCGGTGATG 
CTGGAACATG ATCCGCAGTA TGCAGTAATT TTGGCCTTTG ATGTAAGAAT CGAACGGGAT 
GCGCAAGAGA TGGCTGATAG TTTGGGAGTT AGAATCTTTA GTGCAGAAAT TATTTATCAT 
TTATTTGATG CCTTTACAAA ATACAGACAA GACTACAAGA AACAAAAACA AGAAGAATTT 
AAGCACATAG CAGTATTTCC CTGCAAGATG AAGATTCTCC CTCAGTACAT TTTCAATTCC 
CGAGATCCAA TAGTAATAGG GGTCACTGTG GAGGCTGGTC AAGTGAAACA AGGAACACCT 
ATGTGTGTTC CAAGCAAAAA TTTTGTTGAC ATTGGGATAG TAACAAGCAT TGAAATAAAC 
CACAAGCAGG TGGATGTTGC AAAAAAAGGA CAAGAGGTCT GTGTCAAAAT AGAACCTATC 
CCTGGGGAAT CTCCAAAAAT GTTTGGGAGA CACTTCGAAG CTACAGACAT TCTTGTCAGC 
AAGATCAGCC GGCAGTCCAT CGATGCACTC AAAGACTGGT TCAGAGACGA AATGCAGAAG 
AGTGACTGGC AGCTTATTGT GGAGCTGAAG AAAGTATTTG AAATCATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103611.1
CDS	149..3799
Translation	MGKKQKNKSEDSTKDDTDLGALAAEIEGAGAAKEQEPQKGKGKKKKEKKKQDFDENDILRELEELSLEAQGIGADRDAATVKPTENNEEESASKQDKKKKGQKGKKTSFDENDSEELEDKDSKSKKPARPNSEVLLSGSEDADDPNKLSKKGKKAQKSTKKRDGSEEDEDNSKRSKERSRVNSSGESGGESDEFLQSRKGQKKNQKNKSVPTIDSGNEDDDSSFKIKTVAQKKAEKKERERKKREEEKAKLRKVKEKEELEKGRKEQSKQREPQKRPDEEVLVLRGTPDAGAASEEKGDIAATLEDDNEGDKKKKDKKKKKTEKDDKEKEKKKGPSKSTVKAIQEALAKLREEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEVTLRHLQAQGVEVPSKDSLPKKRPVYEDKKKKKTPQQLESKEALETVEVSAPVEVVDQGVPEKEETPPSVDAEEDEETEDAGLDDWEAMASDEEREKEGNMIHIEVEENPEEEEEEEEDEDEEDSEDEEDEGDSEGSDGDEEDYKLSDEKDLGKAGDTKPNKDASSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHGKNVNTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINILKSKKCPFIVALNKIDRLYDWKKSPDSDVAVTLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKDDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKMKILPQYIFNSRDPIVIGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Target ORF information:

RefSeq Version	NM_001110141.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic translation initiation factor 5B (Eif5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110141.1

ORF Insert Sequence:

ATGGGGAAGA AACAGAAAAA CAAGAGCGAA GACAGTACCA AGGATGATAC TGACTTGGGC 
GCCTTGGCTG CGGAAATAGA AGGAGCTGGT GCTGCCAAGG AGCAGGAGCC TCAGAAGGGA 
AAGGGGAAGA AGAAAAAGGA GAAAAAGAAG CAAGACTTTG ATGAGAACGA CATTCTGAGA 
GAGCTGGAGG AGTTGTCTCT GGAAGCCCAA GGCATCGGAG CTGACAGAGA TGCCGCTACA 
GTCAAGCCTA CAGAAAACAA TGAAGAAGAA TCTGCCTCAA AACAAGATAA AAAGAAGAAA 
GGACAAAAAG GCAAGAAAAC AAGTTTTGAT GAGAATGATA GTGAAGAATT GGAAGATAAA 
GATTCAAAAT CAAAAAAGCC TGCCAGACCT AACTCAGAGG TGCTCCTCTC TGGGAGTGAG 
GATGCAGATG ATCCTAATAA ACTTTCTAAA AAAGGTAAAA AAGCCCAGAA ATCAACTAAA 
AAACGGGATG GGTCGGAAGA GGATGAAGAC AATAGCAAAA GAAGTAAAGA ACGTTCGAGA 
GTAAACTCCT CAGGTGAAAG TGGTGGTGAG TCTGATGAAT TTTTGCAGTC CAGAAAAGGA 
CAGAAAAAAA ATCAGAAGAA CAAGTCAGTT CCTACCATAG ACAGTGGGAA TGAGGACGAT 
GACTCTTCCT TCAAAATTAA GACGGTGGCC CAGAAGAAGG CAGAAAAGAA AGAGCGTGAG 
AGGAAAAAGA GAGAAGAGGA GAAAGCAAAG TTGCGAAAGG TGAAAGAGAA AGAAGAGCTA 
GAGAAGGGCA GGAAGGAGCA GAGTAAGCAG AGGGAGCCTC AGAAGAGACC GGACGAGGAG 
GTGTTGGTGC TCAGAGGGAC TCCTGATGCA GGTGCTGCCT CAGAAGAGAA AGGAGACATT 
GCTGCAACTC TGGAAGATGA CAATGAAGGA GACAAAAAGA AAAAAGATAA GAAGAAAAAG 
AAAACAGAAA AAGATGATAA AGAAAAAGAG AAGAAGAAAG GCCCAAGCAA ATCCACTGTT 
AAAGCTATCC AGGAAGCTTT GGCTAAGCTC AGAGAGGAGG AGGAGAGACA GAAGAGAGAG 
GAGGAGGAAA GGATTAAGCG GCTTGAAGAA CTAGAAGCCA AACGAAAAGA AGAGGAACGA 
TTAGAACAAG AAAAAAGAGA AAGGAAAAAG CAAAAAGAAA AAGAAAGAAA AGAACGGTTA 
AAAAAAGAAG GAAAACTGTT AACTAAGTCC CAGAGAGAAG CCAGAGCCAG AGCTGAAGTG 
ACCCTGAGAC ACCTCCAAGC GCAGGGTGTT GAAGTGCCAT CAAAAGACTC GTTGCCAAAG 
AAGAGGCCAG TGTATGAAGA TAAGAAGAAG AAGAAAACAC CACAGCAGCT GGAGAGTAAA 
GAAGCACTTG AGACTGTGGA AGTCAGTGCC CCTGTGGAAG TTGTGGACCA AGGAGTACCA 
GAAAAGGAGG AGACACCGCC TTCTGTTGAC GCAGAGGAAG ACGAAGAAAC TGAAGATGCT 
GGGCTCGATG ACTGGGAAGC TATGGCCAGT GATGAGGAGA GAGAAAAAGA AGGAAATATG 
ATTCACATAG AAGTAGAAGA AAACCCTGAG GAAGAGGAGG AGGAAGAAGA GGATGAAGAT 
GAAGAAGACA GTGAAGATGA GGAAGACGAA GGGGACAGTG AGGGCAGCGA TGGGGACGAG 
GAGGACTACA AGCTATCGGA CGAGAAGGAC TTGGGGAAAG CTGGAGACAC GAAGCCCAAC 
AAAGACGCTA GCTCAGACTC CGAGTATGAC TCTGATGACG ATCGGACTAA AGAAGAGCGG 
GCGTACGACA AAGCAAAACG GAGAATTGAG AAACGACGAC TTGAACATGG TAAAAATGTA 
AATACAGAAA AGCTAAGAGC CCCTATCATC TGTGTGCTTG GACATGTAGA CACAGGGAAG 
ACAAAAATTC TAGATAAGCT CCGCCACACT CATGTACAAG ATGGTGAAGC AGGTGGCATC 
ACACAGCAGA TTGGTGCCAC AAATGTTCCT CTCGAAGCTA TTAATGAACA AACTAAAATG 
ATTAAAAATT TTGACAGAGA GAATGTCCGC ATTCCAGGGA TGCTGATTAT TGACACTCCT 
GGACATGAGT CTTTCAGTAA CCTGAGAAAC AGAGGAAGCT CTCTCTGTGA TATTGCCATT 
TTAGTTGTTG ACATTATGCA TGGTTTAGAG CCCCAGACAA TTGAATCAAT CAACATTCTC 
AAATCCAAAA AATGTCCCTT TATTGTTGCA CTTAATAAGA TTGACAGGTT ATATGATTGG 
AAGAAGAGTC CTGATTCGGA TGTGGCTGTT ACTTTAAAAA AGCAGAAGAA GAATACAAAG 
GATGAGTTTG AGGAGCGTGC AAAGGCCATT ATTGTAGAAT TTGCACAACA GGGTTTAAAT 
GCTGCTTTGT TTTATGAGAA TAAAGATCCC CGCACTTTTG TGTCCCTGGT ACCGACCTCT 
GCACACACTG GTGATGGCAT GGGAAGCCTC ATCTACCTTC TTGTGGAATT AACTCAAACT 
ATGTTGAGCA AGAGGCTTGC TCACTGTGAG GAGCTGAGAG CACAAGTCAT GGAGGTTAAA 
GCTCTCCCAG GAATGGGCAC CACAATAGAT GTCATATTGA TTAATGGACG TTTGAAGGAA 
GGGGACACAA TCATTGTTCC TGGAGTAGAA GGGCCCATTG TCACTCAGAT TCGAGGCCTC 
CTTTTACCTC CTCCTATGAA GGAATTACGC GTGAAGAACC AATATGAAAA GCATAAAGAA 
GTGGAAGCAG CCCAGGGAGT GAAGATTCTT GGAAAAGACC TTGAGAAAAC GCTGGCTGGT 
TTACCTCTCC TAGTGGCCTA TAAGGACGAC GAAATCCCAG TTCTCAAAGA TGAATTGATC 
CATGAGTTAA AGCAGACGCT GAATGCTATC AAATTGGAAG AAAAAGGAGT CTATGTTCAG 
GCATCTACAC TGGGCTCTTT GGAAGCTCTT CTTGAATTTC TGAAAACATC AGAGGTGCCA 
TATGCGGGAA TTAACATTGG CCCAGTCCAC AAGAAGGATG TTATGAAGGC TTCGGTGATG 
CTGGAACATG ATCCGCAGTA TGCAGTAATT TTGGCCTTTG ATGTAAGAAT CGAACGGGAT 
GCGCAAGAGA TGGCTGATAG TTTGGGAGTT AGAATCTTTA GTGCAGAAAT TATTTATCAT 
TTATTTGATG CCTTTACAAA ATACAGACAA GACTACAAGA AACAAAAACA AGAAGAATTT 
AAGCACATAG CAGTATTTCC CTGCAAGATG AAGATTCTCC CTCAGTACAT TTTCAATTCC 
CGAGATCCAA TAGTAATAGG GGTCACTGTG GAGGCTGGTC AAGTGAAACA AGGAACACCT 
ATGTGTGTTC CAAGCAAAAA TTTTGTTGAC ATTGGGATAG TAACAAGCAT TGAAATAAAC 
CACAAGCAGG TGGATGTTGC AAAAAAAGGA CAAGAGGTCT GTGTCAAAAT AGAACCTATC 
CCTGGGGAAT CTCCAAAAAT GTTTGGGAGA CACTTCGAAG CTACAGACAT TCTTGTCAGC 
AAGATCAGCC GGCAGTCCAT CGATGCACTC AAAGACTGGT TCAGAGACGA AATGCAGAAG 
AGTGACTGGC AGCTTATTGT GGAGCTGAAG AAAGTATTTG AAATCATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanhäusser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm.
The EMBO journal21(22)6205-15(2002 Nov)
Bohnsack MT,Regener K,Schwappach B,Saffrich R,Paraskeva E,Hartmann E,Görlich D

Cloning and characterization of hIF2, a human homologue of bacterial translation initiation factor 2, and its interaction with HIV-1 matrix.
The Biochemical journal342 ( Pt 1)97-103(1999 Aug)
Wilson SA,Sieiro-Vazquez C,Edwards NJ,Iourin O,Byles ED,Kotsopoulou E,Adamson CS,Kingsman SM,Kingsman AJ,Martin-Rendon E

Molecular cloning and characterization of annexin V-binding proteins with highly hydrophilic peptide structure.
Journal of neurochemistry67(1)89-97(1996 Jul)
Ohsawa K,Imai Y,Ito D,Kohsaka S

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Eif5b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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