Amt cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Amt
Entrez Gene ID	306586
Full Name	aminomethyltransferase
General protein information	Preferred Names aminomethyltransferase Names aminomethyltransferase, mitochondrial aminomethyltransferase (glycine cleavage system protein T)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014004.1	NP_001014026.1	aminomethyltransferase, mitochondrial

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Amt	NP_001013836.1
Gallus gallus (chicken)	AMT	NP_990119.1
Pan troglodytes (chimpanzee)	AMT	XP_517018.2
Caenorhabditis elegans (roundworm)	CELE_F25B4.1	NP_504502.1
Saccharomyces cerevisiae (baker's yeast)	GCV1	NP_010302.1
Magnaporthe oryzae (rice blast fungus)	MGG_04826	XP_003713755.1
Xenopus tropicalis (tropical clawed frog)	amt	XP_002936519.1
Neurospora crassa	NCU02727	XP_965112.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001124	XP_322034.3
Schizosaccharomyces pombe (fission yeast)	gcv1	NP_594015.1
Macaca mulatta (Rhesus monkey)	AMT	XP_001108852.1
Drosophila melanogaster (fruit fly)	CG6415	NP_609441.1
Canis lupus familiaris (dog)	AMT	NP_001029165.1
Bos taurus (cattle)	AMT	NP_803451.1
Rattus norvegicus (Norway rat)	Amt	NP_001014026.1
Arabidopsis thaliana (thale cress)	AT1G11860	NP_001184969.1
Oryza sativa (rice)	Os04g0623800	NP_001053928.1
Kluyveromyces lactis	KLLA0B06754g	XP_451833.1
Homo sapiens (human)	AMT	NP_000472.2
Danio rerio (zebrafish)	amt	NP_001006021.1

Gene Ontology
Bibliography

Related articles in PubMed

Compartmentalization of Mammalian folate-mediated one-carbon metabolism.
Tibbetts AS, Appling DR
Annual review of nutrition3057-81(2010 Aug)

Crystal structure of human T-protein of glycine cleavage system at 2.0 A resolution and its implication for understanding non-ketotic hyperglycinemia.
Okamura-Ikeda K, Hosaka H, Yoshimura M, Yamashita E, Toma S, Nakagawa A, Fujiwara K, Motokawa Y, Taniguchi H
Journal of molecular biology351(5)1146-59(2005 Sep)

A missense mutation (His42Arg) in the T-protein gene from a large Israeli-Arab kindred with nonketotic hyperglycinemia.
Kure S, Mandel H, Rolland MO, Sakata Y, Shinka T, Drugan A, Boneh A, Tada K, Matsubara Y, Narisawa K
Human genetics102(4)430-4(1998 Apr)

A one-base deletion (183delC) and a missense mutation (D276H) in the T-protein gene from a Japanese family with nonketotic hyperglycinemia.
Kure S, Shinka T, Sakata Y, Osamu N, Takayanagi M, Tada K, Matsubara Y, Narisawa K
Journal of human genetics43(2)135-7(1998)

Identification of the mutations in the T-protein gene causing typical and atypical nonketotic hyperglycinemia.
Nanao K, Okamura-Ikeda K, Motokawa Y, Danks DM, Baumgartner ER, Takada G, Hayasaka K
Human genetics93(6)655-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Amt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Amt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04568	NM_001014004.1 Latest version!	Rattus norvegicus aminomethyltransferase (Amt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Amt ( NM_001014004.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001014026.1 Rattus norvegicus aminomethyltransferase (Amt), mRNA.

Price & Availability↑

CloneID	ORa04568
Clone ID Related Accession (Same CDS sequence)	NM_001014004.1
Accession Version	NM_001014004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527264000000
Organism	Rattus norvegicus(Norway rat)
Product	aminomethyltransferase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083803.1. On Apr 20, 2005 this sequence version replaced XM_224997.3. ##Evidence-Data-START## Transcript exon combination :: BC083803.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCAGCGGA CAGTCAGGGT GGTAGCCCCT CTGGGTTTTC GCCTTCAGGC ACAACCCTTG 
GTCCAGAGTC GTCCATTCAG TTCTGTACAG GATGGACTCC GCAGGACTCC ACTCTATGAC 
TTCCACCTAG CCCATGGAGG GAAAATGGTG GCGTTTGCAG GGTGGAGTCT GCCTGTGCAA 
TACCGGGACA GTCATGTTGA TTCACACCTG CACACACGCC GTCACTGCTC TCTCTTTGAT 
GTGTCCCACA TGCTACAGAC CAAGATATTT GGCTGTGACC GAGTGAGGCT GATGGAGAAT 
ATAGTGGTTG GAGACATTGC GGAACTAAGG CCTAACCAGG GAACGCTGTC TCTGTTTACT 
AATGAAGCTG GAGGCATCCT AGACGACTTG ATTGTGACCA ATACTTCTGA GGGGCACCTG 
TATGTAGTAT CCAATGCTGG CTGCCGGGAC AAGGACTTGG CTCTCATGCA GGACAAGGTC 
AAGGAGTTCC AGAACAGGGG CCTTGATGTA GGCCTGGAGG TGGTAGATAA TGCCCTGTTA 
GCTCTGCAAG GACCCACTGC AGCACAGGTC TTACAGGCTG GGGTGACAGA TGATATGAGG 
AAACTGCCCT TCATGACCAG TGCTGTGATG GAGGTGTTCG GTGTGTCTGG CTGTCGTGTG 
ACCCGCTGTG GCTACACAGG AGAGGATGGT GTTGAGATAT CAGTGCCAGC GGCAGGAGCA 
GTCCACTTGG CCACTGCTCT GCTGAAAAAC CCAGAGGTAA AGCTGGCAGG ACTAGCAGCC 
CGAGATAGCC TGCGCCTGGA GGCTGGCCTC TGTCTGTATG GGAATGACAT CGATGAACAT 
ACCACACCTG TGGAGGGCAG CCTCAGCTGG ACACTGGGAA AGCGCCGCCG AGTTGCTATG 
GACTTCCCAG GAGCCAAAAT CATCATCCCC CAGCTGAAGG GTGAGGTGCA GAGGCGACGT 
GTGGGGTTGA TATGCGAAGG GGCTCCAATG AGAGCACACA GTCCTATTTT GAACACAGAA 
GGCGCAGTGA TTGGTACAGT GACCAGTGGC TGCCCTTCAC CCAGCCTGAA GAAGAATGTG 
GCGATGGGTT ATGTGGCCTT CAAGTACAGT CGGCCAGGGA CACAGCTGCT GGTGGAAGTT 
CGGCGGAAGC AGCAAATGAC GGTGGTTAGC AAAATGCCCT TTGTGCCCAC GAAGTACTAT 
ACTCTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014026.1
CDS	195..1406
Translation	MQRTVRVVAPLGFRLQAQPLVQSRPFSSVQDGLRRTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHVDSHLHTRRHCSLFDVSHMLQTKIFGCDRVRLMENIVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCRDKDLALMQDKVKEFQNRGLDVGLEVVDNALLALQGPTAAQVLQAGVTDDMRKLPFMTSAVMEVFGVSGCRVTRCGYTGEDGVEISVPAAGAVHLATALLKNPEVKLAGLAARDSLRLEAGLCLYGNDIDEHTTPVEGSLSWTLGKRRRVAMDFPGAKIIIPQLKGEVQRRRVGLICEGAPMRAHSPILNTEGAVIGTVTSGCPSPSLKKNVAMGYVAFKYSRPGTQLLVEVRRKQQMTVVSKMPFVPTKYYTLK

Target ORF information:

RefSeq Version	NM_001014004.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aminomethyltransferase (Amt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014004.1

ORF Insert Sequence:

ATGCAGCGGA CAGTCAGGGT GGTAGCCCCT CTGGGTTTTC GCCTTCAGGC ACAACCCTTG 
GTCCAGAGTC GTCCATTCAG TTCTGTACAG GATGGACTCC GCAGGACTCC ACTCTATGAC 
TTCCACCTAG CCCATGGAGG GAAAATGGTG GCGTTTGCAG GGTGGAGTCT GCCTGTGCAA 
TACCGGGACA GTCATGTTGA TTCACACCTG CACACACGCC GTCACTGCTC TCTCTTTGAT 
GTGTCCCACA TGCTACAGAC CAAGATATTT GGCTGTGACC GAGTGAGGCT GATGGAGAAT 
ATAGTGGTTG GAGACATTGC GGAACTAAGG CCTAACCAGG GAACGCTGTC TCTGTTTACT 
AATGAAGCTG GAGGCATCCT AGACGACTTG ATTGTGACCA ATACTTCTGA GGGGCACCTG 
TATGTAGTAT CCAATGCTGG CTGCCGGGAC AAGGACTTGG CTCTCATGCA GGACAAGGTC 
AAGGAGTTCC AGAACAGGGG CCTTGATGTA GGCCTGGAGG TGGTAGATAA TGCCCTGTTA 
GCTCTGCAAG GACCCACTGC AGCACAGGTC TTACAGGCTG GGGTGACAGA TGATATGAGG 
AAACTGCCCT TCATGACCAG TGCTGTGATG GAGGTGTTCG GTGTGTCTGG CTGTCGTGTG 
ACCCGCTGTG GCTACACAGG AGAGGATGGT GTTGAGATAT CAGTGCCAGC GGCAGGAGCA 
GTCCACTTGG CCACTGCTCT GCTGAAAAAC CCAGAGGTAA AGCTGGCAGG ACTAGCAGCC 
CGAGATAGCC TGCGCCTGGA GGCTGGCCTC TGTCTGTATG GGAATGACAT CGATGAACAT 
ACCACACCTG TGGAGGGCAG CCTCAGCTGG ACACTGGGAA AGCGCCGCCG AGTTGCTATG 
GACTTCCCAG GAGCCAAAAT CATCATCCCC CAGCTGAAGG GTGAGGTGCA GAGGCGACGT 
GTGGGGTTGA TATGCGAAGG GGCTCCAATG AGAGCACACA GTCCTATTTT GAACACAGAA 
GGCGCAGTGA TTGGTACAGT GACCAGTGGC TGCCCTTCAC CCAGCCTGAA GAAGAATGTG 
GCGATGGGTT ATGTGGCCTT CAAGTACAGT CGGCCAGGGA CACAGCTGCT GGTGGAAGTT 
CGGCGGAAGC AGCAAATGAC GGTGGTTAGC AAAATGCCCT TTGTGCCCAC GAAGTACTAT 
ACTCTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Compartmentalization of Mammalian folate-mediated one-carbon metabolism.
Annual review of nutrition3057-81(2010 Aug)
Tibbetts AS,Appling DR

Crystal structure of human T-protein of glycine cleavage system at 2.0 A resolution and its implication for understanding non-ketotic hyperglycinemia.
Journal of molecular biology351(5)1146-59(2005 Sep)
Okamura-Ikeda K,Hosaka H,Yoshimura M,Yamashita E,Toma S,Nakagawa A,Fujiwara K,Motokawa Y,Taniguchi H

A missense mutation (His42Arg) in the T-protein gene from a large Israeli-Arab kindred with nonketotic hyperglycinemia.
Human genetics102(4)430-4(1998 Apr)
Kure S,Mandel H,Rolland MO,Sakata Y,Shinka T,Drugan A,Boneh A,Tada K,Matsubara Y,Narisawa K

A one-base deletion (183delC) and a missense mutation (D276H) in the T-protein gene from a Japanese family with nonketotic hyperglycinemia.
Journal of human genetics43(2)135-7(1998)
Kure S,Shinka T,Sakata Y,Osamu N,Takayanagi M,Tada K,Matsubara Y,Narisawa K

Identification of the mutations in the T-protein gene causing typical and atypical nonketotic hyperglycinemia.
Human genetics93(6)655-8(1994 Jun)
Nanao K,Okamura-Ikeda K,Motokawa Y,Danks DM,Baumgartner ER,Takada G,Hayasaka K

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Amt cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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