Trmt10c cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trmt10c
Entrez Gene ID	304012
Full Name	tRNA methyltransferase 10C, mitochondrial RNase P subunit
Synonyms	Rg9mtd1
General protein information	Preferred Names tRNA methyltransferase 10C, mitochondrial RNase P subunit Names tRNA methyltransferase 10 homolog C RNA (guanine-9-) methyltransferase domain containing 1 RNA (guanine-9-)-methyltransferase domain-containing protein 1 mRNA methyladenosine-N(1)-methyltransferase mitochondrial RNase P protein 1 mitochondrial ribonuclease P protein 1 tRNA (adenine(9)-N(1))-methyltransferase tRNA (guanine(9)-N(1))-methyltransferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008337.1	NP_001008338.1	tRNA methyltransferase 10 homolog C precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRMT10C	NP_060289.2
Macaca mulatta (Rhesus monkey)	LOC702107	XP_001090390.1
Xenopus tropicalis (tropical clawed frog)	trmt10c	NP_001096307.1
Bos taurus (cattle)	TRMT10C	NP_001039788.1
Mus musculus (house mouse)	Trmt10c	NP_083368.1
Caenorhabditis elegans (roundworm)	F46F11.10	NP_491647.3
Canis lupus familiaris (dog)	TRMT10C	XP_535722.3
Gallus gallus (chicken)	TRMT10C	XP_001232374.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007211	XP_308574.4
Pan troglodytes (chimpanzee)	RG9MTD1	NP_001230873.1
Rattus norvegicus (Norway rat)	Trmt10c	NP_001008338.1
Danio rerio (zebrafish)	trmt10c	NP_001007333.2
Drosophila melanogaster (fruit fly)	CG5190	NP_611335.1

Gene Ontology
Bibliography

Related articles in PubMed

The MRPP1/MRPP2 complex is a tRNA-maturation platform in human mitochondria.
Reinhard L, Sridhara S, Hällberg BM
Nucleic acids research45(21)12469-12480(2017 Dec)

The m1A landscape on cytosolic and mitochondrial mRNA at single-base resolution.
Safra M, Sas-Chen A, Nir R, Winkler R, Nachshon A, Bar-Yaacov D, Erlacher M, Rossmanith W, Stern-Ginossar N, Schwartz S
Nature551(7679)251-255(2017 11)

Recessive Mutations in TRMT10C Cause Defects in Mitochondrial RNA Processing and Multiple Respiratory Chain Deficiencies.
Metodiev MD, Thompson K, Alston CL, Morris AAM, He L, Assouline Z, Rio M, Bahi-Buisson N, Pyle A, Griffin H, Siira S, Filipovska A, Munnich A, Chinnery PF, McFarland R, Rötig A, Taylor RW
American journal of human genetics98(5)993-1000(2016 May)

Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.
Bogenhagen DF, Martin DW, Koller A
Cell metabolism19(4)618-29(2014 Apr)

A subcomplex of human mitochondrial RNase P is a bifunctional methyltransferase--extensive moonlighting in mitochondrial tRNA biogenesis.
Vilardo E, Nachbagauer C, Buzet A, Taschner A, Holzmann J, Rossmanith W
Nucleic acids research40(22)11583-93(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Trmt10c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trmt10c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12811	NM_001008337.1 Latest version!	Rattus norvegicus tRNA methyltransferase 10C, mitochondrial RNase P subunit (Trmt10c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trmt10c ( NM_001008337.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001008338.1 Rattus norvegicus tRNA methyltransferase 10C, mitochondrial RNase P subunit (Trmt10c), mRNA.

Price & Availability↑

CloneID	ORa12811
Clone ID Related Accession (Same CDS sequence)	NM_001008337.1
Accession Version	NM_001008337.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559836800000
Organism	Rattus norvegicus(Norway rat)
Product	tRNA methyltransferase 10 homolog C precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085895.1. On Dec 16, 2004 this sequence version replaced XM_221564.2. ##Evidence-Data-START## Transcript exon combination :: BC085895.1, CK603493.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGAATGTGA CTGTTCGTTT CTTAAGACCT TTTGCCAGAT ATCTGGTGCC ATATACCTTC 
CATAGGACAA GAAGTAATTC ATATTCAAGA GTTCTGCAGA GATATGTGTC TTCCAAAGTA 
CCTTCTTTGC CCTGTCATAA TAAGGACAGT ACATCCCCTC CTGAGCAGCT AGAATTGGAT 
GGGTGGAAAA CTACAATGAA ATCTAGCATT CAAGAAAATG GTGTTTCAGT GGTCTCCGAC 
AAAGATGAAG ACTCTCTGGC TGCAACCAGA GAGCTAATTG AGATGTGGAG ATTGCTTGGC 
AAAGAAGTAC CAGAACACAT CACTGAAGAG GAGCTCAAAA CCCTTATGGA ATGTGCTTCT 
AAGTCAGCCA AAAAGAAATA CTTAAGGTAT TTGTATGGTA AGGAAATGAT GAAAAAAGCT 
AAGCAAATGA AGAAGGAAAT GAAAGCAGCA GCCAGGGAAG AAGCAAAAAG GGCCAGGTCA 
CTAGAGCCCA GCACGGGAGA GGAACAACGG GACTTCATGT TTCTCCGACT CTGGGACCGA 
CAGACCAACA TCGCACTGGG ATGGAAGGGT GTCCAGGCCA TGCAGTTTGG ACAGCCTTTG 
GTTTTTGACA TGGCTTATGA TAATTACATG AAACCAAGTG AACTGCAGAA TACAGTTTCT 
CAACTTTTAG AAAGTGAAGG CTGGAACAGA AGGAATGTTG ATCCTTTCCA CATTTATTTC 
TGCAATCTTG AGGTAGATGG TGCTTACCAT AGAGAGCTAG TTAAACGGTA TGGAGAAAAA 
TGGGACAAGT TGCTCTTAAC AGCCACAGAA AAGTCTCCTG TCGATTTATT TCCAAAGGAC 
AGTATTATAT ATTTAACTGC AGATTCTCCC AATGTTATGA CTACCTTCAA GCATGATAAA 
ATTTATATAA TTGGATCATT TGTTGATAAA AATACACAGA CAGGAACATC CTTAGCCAAG 
GCTAAACGGC AAAATCTAGC AACAGAATGC CTTCCACTAG ATAAATATTT ACAATGGGAT 
GTTGGTAACA AAAACCTCAC CTTAGATCAA ATGATTCGAA TTCTGCTCTG CCTGAAAAAC 
ACTGGTAACT GGGAAGAGGC TCTCAAGTTT GTTCCCAGGA GAAAGCACAC TGGCTATTTA 
GAGGTTCCTG AGCATTCGCA GGCAGCTTTT AGGAAACTGA AGAAGACAAA GACTTTAAAT 
TCGTTTCGAA AGGGCTCCTT AAATGTACAC ATGTGGAAAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008338.1
CDS	69..1313
Translation	MNVTVRFLRPFARYLVPYTFHRTRSNSYSRVLQRYVSSKVPSLPCHNKDSTSPPEQLELDGWKTTMKSSIQENGVSVVSDKDEDSLAATRELIEMWRLLGKEVPEHITEEELKTLMECASKSAKKKYLRYLYGKEMMKKAKQMKKEMKAAAREEAKRARSLEPSTGEEQRDFMFLRLWDRQTNIALGWKGVQAMQFGQPLVFDMAYDNYMKPSELQNTVSQLLESEGWNRRNVDPFHIYFCNLEVDGAYHRELVKRYGEKWDKLLLTATEKSPVDLFPKDSIIYLTADSPNVMTTFKHDKIYIIGSFVDKNTQTGTSLAKAKRQNLATECLPLDKYLQWDVGNKNLTLDQMIRILLCLKNTGNWEEALKFVPRRKHTGYLEVPEHSQAAFRKLKKTKTLNSFRKGSLNVHMWKR

Target ORF information:

RefSeq Version	NM_001008337.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tRNA methyltransferase 10C, mitochondrial RNase P subunit (Trmt10c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008337.1

ORF Insert Sequence:

ATGAATGTGA CTGTTCGTTT CTTAAGACCT TTTGCCAGAT ATCTGGTGCC ATATACCTTC 
CATAGGACAA GAAGTAATTC ATATTCAAGA GTTCTGCAGA GATATGTGTC TTCCAAAGTA 
CCTTCTTTGC CCTGTCATAA TAAGGACAGT ACATCCCCTC CTGAGCAGCT AGAATTGGAT 
GGGTGGAAAA CTACAATGAA ATCTAGCATT CAAGAAAATG GTGTTTCAGT GGTCTCCGAC 
AAAGATGAAG ACTCTCTGGC TGCAACCAGA GAGCTAATTG AGATGTGGAG ATTGCTTGGC 
AAAGAAGTAC CAGAACACAT CACTGAAGAG GAGCTCAAAA CCCTTATGGA ATGTGCTTCT 
AAGTCAGCCA AAAAGAAATA CTTAAGGTAT TTGTATGGTA AGGAAATGAT GAAAAAAGCT 
AAGCAAATGA AGAAGGAAAT GAAAGCAGCA GCCAGGGAAG AAGCAAAAAG GGCCAGGTCA 
CTAGAGCCCA GCACGGGAGA GGAACAACGG GACTTCATGT TTCTCCGACT CTGGGACCGA 
CAGACCAACA TCGCACTGGG ATGGAAGGGT GTCCAGGCCA TGCAGTTTGG ACAGCCTTTG 
GTTTTTGACA TGGCTTATGA TAATTACATG AAACCAAGTG AACTGCAGAA TACAGTTTCT 
CAACTTTTAG AAAGTGAAGG CTGGAACAGA AGGAATGTTG ATCCTTTCCA CATTTATTTC 
TGCAATCTTG AGGTAGATGG TGCTTACCAT AGAGAGCTAG TTAAACGGTA TGGAGAAAAA 
TGGGACAAGT TGCTCTTAAC AGCCACAGAA AAGTCTCCTG TCGATTTATT TCCAAAGGAC 
AGTATTATAT ATTTAACTGC AGATTCTCCC AATGTTATGA CTACCTTCAA GCATGATAAA 
ATTTATATAA TTGGATCATT TGTTGATAAA AATACACAGA CAGGAACATC CTTAGCCAAG 
GCTAAACGGC AAAATCTAGC AACAGAATGC CTTCCACTAG ATAAATATTT ACAATGGGAT 
GTTGGTAACA AAAACCTCAC CTTAGATCAA ATGATTCGAA TTCTGCTCTG CCTGAAAAAC 
ACTGGTAACT GGGAAGAGGC TCTCAAGTTT GTTCCCAGGA GAAAGCACAC TGGCTATTTA 
GAGGTTCCTG AGCATTCGCA GGCAGCTTTT AGGAAACTGA AGAAGACAAA GACTTTAAAT 
TCGTTTCGAA AGGGCTCCTT AAATGTACAC ATGTGGAAAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The MRPP1/MRPP2 complex is a tRNA-maturation platform in human mitochondria.
Nucleic acids research45(21)12469-12480(2017 Dec)
Reinhard L,Sridhara S,Hällberg BM

The m1A landscape on cytosolic and mitochondrial mRNA at single-base resolution.
Nature551(7679)251-255(2017 11)
Safra M,Sas-Chen A,Nir R,Winkler R,Nachshon A,Bar-Yaacov D,Erlacher M,Rossmanith W,Stern-Ginossar N,Schwartz S

Recessive Mutations in TRMT10C Cause Defects in Mitochondrial RNA Processing and Multiple Respiratory Chain Deficiencies.
American journal of human genetics98(5)993-1000(2016 May)
Metodiev MD,Thompson K,Alston CL,Morris AAM,He L,Assouline Z,Rio M,Bahi-Buisson N,Pyle A,Griffin H,Siira S,Filipovska A,Munnich A,Chinnery PF,McFarland R,Rötig A,Taylor RW

Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.
Cell metabolism19(4)618-29(2014 Apr)
Bogenhagen DF,Martin DW,Koller A

A subcomplex of human mitochondrial RNase P is a bifunctional methyltransferase--extensive moonlighting in mitochondrial tRNA biogenesis.
Nucleic acids research40(22)11583-93(2012 Dec)
Vilardo E,Nachbagauer C,Buzet A,Taschner A,Holzmann J,Rossmanith W

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Trmt10c cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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