Ddx42 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx42
Entrez Gene ID	303607
Full Name	DEAD-box helicase 42
General protein information	Preferred Names DEAD-box helicase 42 Names ATP-dependent RNA helicase DDX42 DEAD (Asp-Glu-Ala-Asp) box helicase 42 DEAD (Asp-Glu-Ala-Asp) box polypeptide 42
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006247596.3	XP_006247658.1	ATP-dependent RNA helicase DDX42 isoform X1
XM_006247597.3	XP_006247659.1	ATP-dependent RNA helicase DDX42 isoform X1
NM_001107059.1	NP_001100529.1	ATP-dependent RNA helicase DDX42

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ddx42	NP_082350.3
Rattus norvegicus (Norway rat)	Ddx42	NP_001100529.1
Caenorhabditis elegans (roundworm)	C46F11.4	NP_497743.1
Canis lupus familiaris (dog)	DDX42	XP_537598.3
Danio rerio (zebrafish)	ddx42	NP_001032894.2
Homo sapiens (human)	DDX42	NP_031398.2
Pan troglodytes (chimpanzee)	DDX42	XP_001147880.1
Arabidopsis thaliana (thale cress)	AT2G47330	NP_566099.1
Bos taurus (cattle)	DDX42	XP_003587506.2
Xenopus tropicalis (tropical clawed frog)	ddx42	XP_004918655.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012523	XP_306246.4
Macaca mulatta (Rhesus monkey)	DDX42	XP_001116381.1
Drosophila melanogaster (fruit fly)	CG6418	NP_648413.1
Oryza sativa (rice)	Os03g0308500	NP_001049904.1
Gallus gallus (chicken)	DDX42	NP_001026097.1

Gene Ontology
Bibliography

Related articles in PubMed

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanhäusser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

The DEAD box protein Ddx42p modulates the function of ASPP2, a stimulator of apoptosis.
Uhlmann-Schiffler H, Kiermayer S, Stahl H
Oncogene28(20)2065-73(2009 May)

Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors.
Chen YI, Moore RE, Ge HY, Young MK, Lee TD, Stevens SW
Nucleic acids research35(12)3928-44(2007)

Gene expression profiling of the bone marrow mononuclear cells from patients with myelodysplastic syndrome.
Qian J, Chen Z, Wang W, Cen J, Xue Y
Oncology reports14(5)1189-97(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ddx42 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx42 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02801	NM_001107059.1 Latest version!	Rattus norvegicus DEAD-box helicase 42 (Ddx42), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa02801	XM_006247596.3 Latest version!	Rattus norvegicus DEAD-box helicase 42 (Ddx42), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa02801	XM_006247597.3 Latest version!	Rattus norvegicus DEAD-box helicase 42 (Ddx42), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx42 ( NM_001107059.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100529.1 Rattus norvegicus DEAD-box helicase 42 (Ddx42), mRNA.

Price & Availability↑

CloneID	ORa02801
Clone ID Related Accession (Same CDS sequence)	XM_006247596.3 , XM_006247597.3 , NM_001107059.1
Accession Version	NM_001107059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent RNA helicase DDX42
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473948.1. On or before Oct 4, 2007 this sequence version replaced XM_221037.3, XM_001081592.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAATTGGA ATAAAGGTGG ACCTGGAACC AAGCGGGGGT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAGAAGGA GGAAGCCAAG CTGCCGCAGC AGTCGCACAG TGCCTTCGGG 
GCGGCCAGCT CTTCTTCTGG ATTTGGAAAG TCTGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAACGGGC CAACTTTGAT GAAGAAAATG CTTATTTCGA GGATGAAGAA 
GAAGACTCCA GCAATGTAGA CTTACCTTAC ATTCCTGCTG AAAACTCGCC TACCCGCCAG 
CAGTTCCGAT CCAAGCCAGC AGACTCTGAC AGTGACGACG ACCCCTTAGA GGCATTCATG 
GCTGAAGTAG AGGATCAGGC GGCTAGAGAC ATGAAGAGAC TTGAGGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGGGTAGTTC AGGAGGAAGA GGAAGACAAT 
CTGGAGTATG ATAGTGATGG AAATCCAATT GCACCTTCTA AAAAGATTAT CGATCCCCTT 
CCTCCCATTG ATCATTCGGA GATTGACTAT CCACCATTTG AAAAAAATTT TTACAATGAA 
CATGAGGAGA TAACCAACCT CACTCCACAG CAGCTAATAG ACCTGCGGCA TAAACTCAAC 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GTTTTGCTCA TTTTGGGTTT 
GATGAACAAC TTATGCATCA GATTAGGAAA TCTGAGTACA CACAGCCAAC TCCAATACAG 
TGTCAGGGTG TGCCCGTAGC ACTAAGTGGT AGAGACATGA TCGGTATTGC TAAAACAGGC 
AGTGGGAAAA CCGCAGCCTT TATTTGGCCC ATGTTGATTC ACATAATGGA TCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACTAGGGA GCTTTGTCAA 
CAGATCCACG CCGAGTGCAA GCGTTTTGGG AAAGCATATA ATCTTCGATC AGTGGCAGTA 
TATGGAGGAG GGAGCATGTG GGAGCAGGCC AAGGCCCTTC AGGAAGGGGC AGAGATTGTT 
GTGTGTACCC CGGGTCGGCT GATTGATCAT GTGAAGAAAA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCTGAT CGAATGTTCG ACATGGGGTT TGAGTATCAG 
GTTCGGTCCA TAGCAAGTCA TGTCCGTCCT GACAGACAAA CTCTCTTATT CAGTGCAACT 
TTTCGGAAAA AGATTGAAAA ATTGGCCAGA GACATCTTGA TTGATCCTAT CCGAGTGGTA 
CAAGGAGACA TCGGAGAGGC AAATGAAGAC GTAACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTCACTCGG CGTCTGGTAG AGTTTACTTC TTCCGGGAGT 
GTCCTCTTGT TTGTTACCAA GAAAGCCAAC GCTGAAGAAC TGGCCAATAA CCTTAAGCAG 
GAGGGTCATA ATCTTGGTCT GCTCCATGGG GACATGGATC AGAGTGAAAG AAACAAGGTC 
ATTTTAGATT TTAAGAAAAA AGACATCCCC GTCCTGGTGG CCACTGACGT TGCAGCCCGT 
GGTCTGGATA TTCCTTCAAT TAAGACAGTC ATTAACTATG ATGTTGCACG AGACATTGAT 
ACCCATACTC ATAGGATTGG CCGAACAGGA AGAGCTGGTG AGAAGGGTGT AGCCTATACC 
TTACTGACCC CAAAGGACAG CAATTTTGCT GGTGACCTTG TACGGAACCT GGAAGGAGCC 
AACCAACATG TTTCTAAGGA ACTCTTAGAT CTCGCAATGC AGAACGCCTG GTTTCGGAAG 
TCACGCTTCA AAGGAGGGAA AGGCAAAAAG CTGAACATCG GTGGAGGAGG CTTGGGCTAT 
AGAGAGCGGC CTGGGCTGGG CTCTGAGAAT TCGGACCGAG GAAGTAACAA CAATGTAATG 
AGCAATTATG AAGCCTACAA GCCCTCCACA GGAGCCATGG GAGATAGGCT GACAGCAATG 
AAAGCAGCTT TCCAGTCCCA GTATAAGAGT CACTTTGTTG CAGCCAGTTT AAGTAATCAG 
AAGGCCGGAA CCTCTACTGC TGGGGCAAGT GGATGGACTA GTGCAGGAAG CTTAAATTCT 
GTTCCAACTA ATTCAGCACA GCAGGGCCAT AATAGTCCTG ACAATCCCAT CACCAGTTCT 
ACCAAGAACA TCCCAGGCTT CAACAATTCT GGGAATATCA GTAGTGCCCC AGTGACCTAC 
CCTTCTATTG GAGCCCAAGG AGTCAACAAC ACAGCTTCAG GGAGTAACAG CCGTGAAGGG 
ATTGGGGGTG GCAATGGGAA AAGAGAGAGA TATACTGAGA ACCGGGGTGG TGGCCGTCAT 
AGCCATGGAG ACAGTGGCAA TCGACATGGA GATGGTGGCC GCCATGGAGA TGGATACCGT 
TACCCAGAAA GCAGTAGCCG TCATACGGAT GGCCACCGAC ATGGAGAGAC CAGGCACGGA 
GGAAGTGCAA GCAGACATGG AGAAAGCCGG GGTGCAAATG ATGGCAGGAA TGGAGAAAGC 
AGGAAAGAAG GCTTTAATCG TGAAAACAGG ATGGACCCCA AGGTGGACAG CAGCAGGATG 
GACAAAGTGG ACAGCAAGAC AGATAAGACA CCTGATGGCT TTGCTGTTCC AGAGCCACCC 
AAACGCAAGA AAAGCCGATG GGATAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100529.1
CDS	248..3037
Translation	MNWNKGGPGTKRGFGFGGFAISAGKKEEAKLPQQSHSAFGAASSSSGFGKSAPPQLPSFYKIGSKRANFDEENAYFEDEEEDSSNVDLPYIPAENSPTRQQFRSKPADSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPSKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVILDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENSDRGSNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGTSTAGASGWTSAGSLNSVPTNSAQQGHNSPDNPITSSTKNIPGFNNSGNISSAPVTYPSIGAQGVNNTASGSNSREGIGGGNGKRERYTENRGGGRHSHGDSGNRHGDGGRHGDGYRYPESSSRHTDGHRHGETRHGGSASRHGESRGANDGRNGESRKEGFNRENRMDPKVDSSRMDKVDSKTDKTPDGFAVPEPPKRKKSRWDS

Target ORF information:

RefSeq Version	NM_001107059.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 42 (Ddx42), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107059.1

ORF Insert Sequence:

ATGAATTGGA ATAAAGGTGG ACCTGGAACC AAGCGGGGGT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAGAAGGA GGAAGCCAAG CTGCCGCAGC AGTCGCACAG TGCCTTCGGG 
GCGGCCAGCT CTTCTTCTGG ATTTGGAAAG TCTGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAACGGGC CAACTTTGAT GAAGAAAATG CTTATTTCGA GGATGAAGAA 
GAAGACTCCA GCAATGTAGA CTTACCTTAC ATTCCTGCTG AAAACTCGCC TACCCGCCAG 
CAGTTCCGAT CCAAGCCAGC AGACTCTGAC AGTGACGACG ACCCCTTAGA GGCATTCATG 
GCTGAAGTAG AGGATCAGGC GGCTAGAGAC ATGAAGAGAC TTGAGGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGGGTAGTTC AGGAGGAAGA GGAAGACAAT 
CTGGAGTATG ATAGTGATGG AAATCCAATT GCACCTTCTA AAAAGATTAT CGATCCCCTT 
CCTCCCATTG ATCATTCGGA GATTGACTAT CCACCATTTG AAAAAAATTT TTACAATGAA 
CATGAGGAGA TAACCAACCT CACTCCACAG CAGCTAATAG ACCTGCGGCA TAAACTCAAC 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GTTTTGCTCA TTTTGGGTTT 
GATGAACAAC TTATGCATCA GATTAGGAAA TCTGAGTACA CACAGCCAAC TCCAATACAG 
TGTCAGGGTG TGCCCGTAGC ACTAAGTGGT AGAGACATGA TCGGTATTGC TAAAACAGGC 
AGTGGGAAAA CCGCAGCCTT TATTTGGCCC ATGTTGATTC ACATAATGGA TCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACTAGGGA GCTTTGTCAA 
CAGATCCACG CCGAGTGCAA GCGTTTTGGG AAAGCATATA ATCTTCGATC AGTGGCAGTA 
TATGGAGGAG GGAGCATGTG GGAGCAGGCC AAGGCCCTTC AGGAAGGGGC AGAGATTGTT 
GTGTGTACCC CGGGTCGGCT GATTGATCAT GTGAAGAAAA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCTGAT CGAATGTTCG ACATGGGGTT TGAGTATCAG 
GTTCGGTCCA TAGCAAGTCA TGTCCGTCCT GACAGACAAA CTCTCTTATT CAGTGCAACT 
TTTCGGAAAA AGATTGAAAA ATTGGCCAGA GACATCTTGA TTGATCCTAT CCGAGTGGTA 
CAAGGAGACA TCGGAGAGGC AAATGAAGAC GTAACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTCACTCGG CGTCTGGTAG AGTTTACTTC TTCCGGGAGT 
GTCCTCTTGT TTGTTACCAA GAAAGCCAAC GCTGAAGAAC TGGCCAATAA CCTTAAGCAG 
GAGGGTCATA ATCTTGGTCT GCTCCATGGG GACATGGATC AGAGTGAAAG AAACAAGGTC 
ATTTTAGATT TTAAGAAAAA AGACATCCCC GTCCTGGTGG CCACTGACGT TGCAGCCCGT 
GGTCTGGATA TTCCTTCAAT TAAGACAGTC ATTAACTATG ATGTTGCACG AGACATTGAT 
ACCCATACTC ATAGGATTGG CCGAACAGGA AGAGCTGGTG AGAAGGGTGT AGCCTATACC 
TTACTGACCC CAAAGGACAG CAATTTTGCT GGTGACCTTG TACGGAACCT GGAAGGAGCC 
AACCAACATG TTTCTAAGGA ACTCTTAGAT CTCGCAATGC AGAACGCCTG GTTTCGGAAG 
TCACGCTTCA AAGGAGGGAA AGGCAAAAAG CTGAACATCG GTGGAGGAGG CTTGGGCTAT 
AGAGAGCGGC CTGGGCTGGG CTCTGAGAAT TCGGACCGAG GAAGTAACAA CAATGTAATG 
AGCAATTATG AAGCCTACAA GCCCTCCACA GGAGCCATGG GAGATAGGCT GACAGCAATG 
AAAGCAGCTT TCCAGTCCCA GTATAAGAGT CACTTTGTTG CAGCCAGTTT AAGTAATCAG 
AAGGCCGGAA CCTCTACTGC TGGGGCAAGT GGATGGACTA GTGCAGGAAG CTTAAATTCT 
GTTCCAACTA ATTCAGCACA GCAGGGCCAT AATAGTCCTG ACAATCCCAT CACCAGTTCT 
ACCAAGAACA TCCCAGGCTT CAACAATTCT GGGAATATCA GTAGTGCCCC AGTGACCTAC 
CCTTCTATTG GAGCCCAAGG AGTCAACAAC ACAGCTTCAG GGAGTAACAG CCGTGAAGGG 
ATTGGGGGTG GCAATGGGAA AAGAGAGAGA TATACTGAGA ACCGGGGTGG TGGCCGTCAT 
AGCCATGGAG ACAGTGGCAA TCGACATGGA GATGGTGGCC GCCATGGAGA TGGATACCGT 
TACCCAGAAA GCAGTAGCCG TCATACGGAT GGCCACCGAC ATGGAGAGAC CAGGCACGGA 
GGAAGTGCAA GCAGACATGG AGAAAGCCGG GGTGCAAATG ATGGCAGGAA TGGAGAAAGC 
AGGAAAGAAG GCTTTAATCG TGAAAACAGG ATGGACCCCA AGGTGGACAG CAGCAGGATG 
GACAAAGTGG ACAGCAAGAC AGATAAGACA CCTGATGGCT TTGCTGTTCC AGAGCCACCC 
AAACGCAAGA AAAGCCGATG GGATAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx42 ( XM_006247596.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247658.1 Rattus norvegicus DEAD-box helicase 42 (Ddx42), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa02801
Clone ID Related Accession (Same CDS sequence)	XM_006247596.3 , XM_006247597.3 , NM_001107059.1
Accession Version	XM_006247596.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent RNA helicase DDX42 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247596.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTGGA ATAAAGGTGG ACCTGGAACC AAGCGGGGGT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAGAAGGA GGAAGCCAAG CTGCCGCAGC AGTCGCACAG TGCCTTCGGG 
GCGGCCAGCT CTTCTTCTGG ATTTGGAAAG TCTGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAACGGGC CAACTTTGAT GAAGAAAATG CTTATTTCGA GGATGAAGAA 
GAAGACTCCA GCAATGTAGA CTTACCTTAC ATTCCTGCTG AAAACTCGCC TACCCGCCAG 
CAGTTCCGAT CCAAGCCAGC AGACTCTGAC AGTGACGACG ACCCCTTAGA GGCATTCATG 
GCTGAAGTAG AGGATCAGGC GGCTAGAGAC ATGAAGAGAC TTGAGGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGGGTAGTTC AGGAGGAAGA GGAAGACAAT 
CTGGAGTATG ATAGTGATGG AAATCCAATT GCACCTTCTA AAAAGATTAT CGATCCCCTT 
CCTCCCATTG ATCATTCGGA GATTGACTAT CCACCATTTG AAAAAAATTT TTACAATGAA 
CATGAGGAGA TAACCAACCT CACTCCACAG CAGCTAATAG ACCTGCGGCA TAAACTCAAC 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GTTTTGCTCA TTTTGGGTTT 
GATGAACAAC TTATGCATCA GATTAGGAAA TCTGAGTACA CACAGCCAAC TCCAATACAG 
TGTCAGGGTG TGCCCGTAGC ACTAAGTGGT AGAGACATGA TCGGTATTGC TAAAACAGGC 
AGTGGGAAAA CCGCAGCCTT TATTTGGCCC ATGTTGATTC ACATAATGGA TCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACTAGGGA GCTTTGTCAA 
CAGATCCACG CCGAGTGCAA GCGTTTTGGG AAAGCATATA ATCTTCGATC AGTGGCAGTA 
TATGGAGGAG GGAGCATGTG GGAGCAGGCC AAGGCCCTTC AGGAAGGGGC AGAGATTGTT 
GTGTGTACCC CGGGTCGGCT GATTGATCAT GTGAAGAAAA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCTGAT CGAATGTTCG ACATGGGGTT TGAGTATCAG 
GTTCGGTCCA TAGCAAGTCA TGTCCGTCCT GACAGACAAA CTCTCTTATT CAGTGCAACT 
TTTCGGAAAA AGATTGAAAA ATTGGCCAGA GACATCTTGA TTGATCCTAT CCGAGTGGTA 
CAAGGAGACA TCGGAGAGGC AAATGAAGAC GTAACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTCACTCGG CGTCTGGTAG AGTTTACTTC TTCCGGGAGT 
GTCCTCTTGT TTGTTACCAA GAAAGCCAAC GCTGAAGAAC TGGCCAATAA CCTTAAGCAG 
GAGGGTCATA ATCTTGGTCT GCTCCATGGG GACATGGATC AGAGTGAAAG AAACAAGGTC 
ATTTTAGATT TTAAGAAAAA AGACATCCCC GTCCTGGTGG CCACTGACGT TGCAGCCCGT 
GGTCTGGATA TTCCTTCAAT TAAGACAGTC ATTAACTATG ATGTTGCACG AGACATTGAT 
ACCCATACTC ATAGGATTGG CCGAACAGGA AGAGCTGGTG AGAAGGGTGT AGCCTATACC 
TTACTGACCC CAAAGGACAG CAATTTTGCT GGTGACCTTG TACGGAACCT GGAAGGAGCC 
AACCAACATG TTTCTAAGGA ACTCTTAGAT CTCGCAATGC AGAACGCCTG GTTTCGGAAG 
TCACGCTTCA AAGGAGGGAA AGGCAAAAAG CTGAACATCG GTGGAGGAGG CTTGGGCTAT 
AGAGAGCGGC CTGGGCTGGG CTCTGAGAAT TCGGACCGAG GAAGTAACAA CAATGTAATG 
AGCAATTATG AAGCCTACAA GCCCTCCACA GGAGCCATGG GAGATAGGCT GACAGCAATG 
AAAGCAGCTT TCCAGTCCCA GTATAAGAGT CACTTTGTTG CAGCCAGTTT AAGTAATCAG 
AAGGCCGGAA CCTCTACTGC TGGGGCAAGT GGATGGACTA GTGCAGGAAG CTTAAATTCT 
GTTCCAACTA ATTCAGCACA GCAGGGCCAT AATAGTCCTG ACAATCCCAT CACCAGTTCT 
ACCAAGAACA TCCCAGGCTT CAACAATTCT GGGAATATCA GTAGTGCCCC AGTGACCTAC 
CCTTCTATTG GAGCCCAAGG AGTCAACAAC ACAGCTTCAG GGAGTAACAG CCGTGAAGGG 
ATTGGGGGTG GCAATGGGAA AAGAGAGAGA TATACTGAGA ACCGGGGTGG TGGCCGTCAT 
AGCCATGGAG ACAGTGGCAA TCGACATGGA GATGGTGGCC GCCATGGAGA TGGATACCGT 
TACCCAGAAA GCAGTAGCCG TCATACGGAT GGCCACCGAC ATGGAGAGAC CAGGCACGGA 
GGAAGTGCAA GCAGACATGG AGAAAGCCGG GGTGCAAATG ATGGCAGGAA TGGAGAAAGC 
AGGAAAGAAG GCTTTAATCG TGAAAACAGG ATGGACCCCA AGGTGGACAG CAGCAGGATG 
GACAAAGTGG ACAGCAAGAC AGATAAGACA CCTGATGGCT TTGCTGTTCC AGAGCCACCC 
AAACGCAAGA AAAGCCGATG GGATAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247658.1
CDS	553..3342
Translation	MNWNKGGPGTKRGFGFGGFAISAGKKEEAKLPQQSHSAFGAASSSSGFGKSAPPQLPSFYKIGSKRANFDEENAYFEDEEEDSSNVDLPYIPAENSPTRQQFRSKPADSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPSKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVILDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENSDRGSNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGTSTAGASGWTSAGSLNSVPTNSAQQGHNSPDNPITSSTKNIPGFNNSGNISSAPVTYPSIGAQGVNNTASGSNSREGIGGGNGKRERYTENRGGGRHSHGDSGNRHGDGGRHGDGYRYPESSSRHTDGHRHGETRHGGSASRHGESRGANDGRNGESRKEGFNRENRMDPKVDSSRMDKVDSKTDKTPDGFAVPEPPKRKKSRWDS

Target ORF information:

RefSeq Version	XM_006247596.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 42 (Ddx42), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247596.3

ORF Insert Sequence:

ATGAATTGGA ATAAAGGTGG ACCTGGAACC AAGCGGGGGT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAGAAGGA GGAAGCCAAG CTGCCGCAGC AGTCGCACAG TGCCTTCGGG 
GCGGCCAGCT CTTCTTCTGG ATTTGGAAAG TCTGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAACGGGC CAACTTTGAT GAAGAAAATG CTTATTTCGA GGATGAAGAA 
GAAGACTCCA GCAATGTAGA CTTACCTTAC ATTCCTGCTG AAAACTCGCC TACCCGCCAG 
CAGTTCCGAT CCAAGCCAGC AGACTCTGAC AGTGACGACG ACCCCTTAGA GGCATTCATG 
GCTGAAGTAG AGGATCAGGC GGCTAGAGAC ATGAAGAGAC TTGAGGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGGGTAGTTC AGGAGGAAGA GGAAGACAAT 
CTGGAGTATG ATAGTGATGG AAATCCAATT GCACCTTCTA AAAAGATTAT CGATCCCCTT 
CCTCCCATTG ATCATTCGGA GATTGACTAT CCACCATTTG AAAAAAATTT TTACAATGAA 
CATGAGGAGA TAACCAACCT CACTCCACAG CAGCTAATAG ACCTGCGGCA TAAACTCAAC 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GTTTTGCTCA TTTTGGGTTT 
GATGAACAAC TTATGCATCA GATTAGGAAA TCTGAGTACA CACAGCCAAC TCCAATACAG 
TGTCAGGGTG TGCCCGTAGC ACTAAGTGGT AGAGACATGA TCGGTATTGC TAAAACAGGC 
AGTGGGAAAA CCGCAGCCTT TATTTGGCCC ATGTTGATTC ACATAATGGA TCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACTAGGGA GCTTTGTCAA 
CAGATCCACG CCGAGTGCAA GCGTTTTGGG AAAGCATATA ATCTTCGATC AGTGGCAGTA 
TATGGAGGAG GGAGCATGTG GGAGCAGGCC AAGGCCCTTC AGGAAGGGGC AGAGATTGTT 
GTGTGTACCC CGGGTCGGCT GATTGATCAT GTGAAGAAAA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCTGAT CGAATGTTCG ACATGGGGTT TGAGTATCAG 
GTTCGGTCCA TAGCAAGTCA TGTCCGTCCT GACAGACAAA CTCTCTTATT CAGTGCAACT 
TTTCGGAAAA AGATTGAAAA ATTGGCCAGA GACATCTTGA TTGATCCTAT CCGAGTGGTA 
CAAGGAGACA TCGGAGAGGC AAATGAAGAC GTAACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTCACTCGG CGTCTGGTAG AGTTTACTTC TTCCGGGAGT 
GTCCTCTTGT TTGTTACCAA GAAAGCCAAC GCTGAAGAAC TGGCCAATAA CCTTAAGCAG 
GAGGGTCATA ATCTTGGTCT GCTCCATGGG GACATGGATC AGAGTGAAAG AAACAAGGTC 
ATTTTAGATT TTAAGAAAAA AGACATCCCC GTCCTGGTGG CCACTGACGT TGCAGCCCGT 
GGTCTGGATA TTCCTTCAAT TAAGACAGTC ATTAACTATG ATGTTGCACG AGACATTGAT 
ACCCATACTC ATAGGATTGG CCGAACAGGA AGAGCTGGTG AGAAGGGTGT AGCCTATACC 
TTACTGACCC CAAAGGACAG CAATTTTGCT GGTGACCTTG TACGGAACCT GGAAGGAGCC 
AACCAACATG TTTCTAAGGA ACTCTTAGAT CTCGCAATGC AGAACGCCTG GTTTCGGAAG 
TCACGCTTCA AAGGAGGGAA AGGCAAAAAG CTGAACATCG GTGGAGGAGG CTTGGGCTAT 
AGAGAGCGGC CTGGGCTGGG CTCTGAGAAT TCGGACCGAG GAAGTAACAA CAATGTAATG 
AGCAATTATG AAGCCTACAA GCCCTCCACA GGAGCCATGG GAGATAGGCT GACAGCAATG 
AAAGCAGCTT TCCAGTCCCA GTATAAGAGT CACTTTGTTG CAGCCAGTTT AAGTAATCAG 
AAGGCCGGAA CCTCTACTGC TGGGGCAAGT GGATGGACTA GTGCAGGAAG CTTAAATTCT 
GTTCCAACTA ATTCAGCACA GCAGGGCCAT AATAGTCCTG ACAATCCCAT CACCAGTTCT 
ACCAAGAACA TCCCAGGCTT CAACAATTCT GGGAATATCA GTAGTGCCCC AGTGACCTAC 
CCTTCTATTG GAGCCCAAGG AGTCAACAAC ACAGCTTCAG GGAGTAACAG CCGTGAAGGG 
ATTGGGGGTG GCAATGGGAA AAGAGAGAGA TATACTGAGA ACCGGGGTGG TGGCCGTCAT 
AGCCATGGAG ACAGTGGCAA TCGACATGGA GATGGTGGCC GCCATGGAGA TGGATACCGT 
TACCCAGAAA GCAGTAGCCG TCATACGGAT GGCCACCGAC ATGGAGAGAC CAGGCACGGA 
GGAAGTGCAA GCAGACATGG AGAAAGCCGG GGTGCAAATG ATGGCAGGAA TGGAGAAAGC 
AGGAAAGAAG GCTTTAATCG TGAAAACAGG ATGGACCCCA AGGTGGACAG CAGCAGGATG 
GACAAAGTGG ACAGCAAGAC AGATAAGACA CCTGATGGCT TTGCTGTTCC AGAGCCACCC 
AAACGCAAGA AAAGCCGATG GGATAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx42 ( XM_006247597.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247659.1 Rattus norvegicus DEAD-box helicase 42 (Ddx42), transcript variant X2, mRNA.

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CloneID	ORa02801
Clone ID Related Accession (Same CDS sequence)	XM_006247596.3 , XM_006247597.3 , NM_001107059.1
Accession Version	XM_006247597.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent RNA helicase DDX42 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247597.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTGGA ATAAAGGTGG ACCTGGAACC AAGCGGGGGT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAGAAGGA GGAAGCCAAG CTGCCGCAGC AGTCGCACAG TGCCTTCGGG 
GCGGCCAGCT CTTCTTCTGG ATTTGGAAAG TCTGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAACGGGC CAACTTTGAT GAAGAAAATG CTTATTTCGA GGATGAAGAA 
GAAGACTCCA GCAATGTAGA CTTACCTTAC ATTCCTGCTG AAAACTCGCC TACCCGCCAG 
CAGTTCCGAT CCAAGCCAGC AGACTCTGAC AGTGACGACG ACCCCTTAGA GGCATTCATG 
GCTGAAGTAG AGGATCAGGC GGCTAGAGAC ATGAAGAGAC TTGAGGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGGGTAGTTC AGGAGGAAGA GGAAGACAAT 
CTGGAGTATG ATAGTGATGG AAATCCAATT GCACCTTCTA AAAAGATTAT CGATCCCCTT 
CCTCCCATTG ATCATTCGGA GATTGACTAT CCACCATTTG AAAAAAATTT TTACAATGAA 
CATGAGGAGA TAACCAACCT CACTCCACAG CAGCTAATAG ACCTGCGGCA TAAACTCAAC 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GTTTTGCTCA TTTTGGGTTT 
GATGAACAAC TTATGCATCA GATTAGGAAA TCTGAGTACA CACAGCCAAC TCCAATACAG 
TGTCAGGGTG TGCCCGTAGC ACTAAGTGGT AGAGACATGA TCGGTATTGC TAAAACAGGC 
AGTGGGAAAA CCGCAGCCTT TATTTGGCCC ATGTTGATTC ACATAATGGA TCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACTAGGGA GCTTTGTCAA 
CAGATCCACG CCGAGTGCAA GCGTTTTGGG AAAGCATATA ATCTTCGATC AGTGGCAGTA 
TATGGAGGAG GGAGCATGTG GGAGCAGGCC AAGGCCCTTC AGGAAGGGGC AGAGATTGTT 
GTGTGTACCC CGGGTCGGCT GATTGATCAT GTGAAGAAAA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCTGAT CGAATGTTCG ACATGGGGTT TGAGTATCAG 
GTTCGGTCCA TAGCAAGTCA TGTCCGTCCT GACAGACAAA CTCTCTTATT CAGTGCAACT 
TTTCGGAAAA AGATTGAAAA ATTGGCCAGA GACATCTTGA TTGATCCTAT CCGAGTGGTA 
CAAGGAGACA TCGGAGAGGC AAATGAAGAC GTAACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTCACTCGG CGTCTGGTAG AGTTTACTTC TTCCGGGAGT 
GTCCTCTTGT TTGTTACCAA GAAAGCCAAC GCTGAAGAAC TGGCCAATAA CCTTAAGCAG 
GAGGGTCATA ATCTTGGTCT GCTCCATGGG GACATGGATC AGAGTGAAAG AAACAAGGTC 
ATTTTAGATT TTAAGAAAAA AGACATCCCC GTCCTGGTGG CCACTGACGT TGCAGCCCGT 
GGTCTGGATA TTCCTTCAAT TAAGACAGTC ATTAACTATG ATGTTGCACG AGACATTGAT 
ACCCATACTC ATAGGATTGG CCGAACAGGA AGAGCTGGTG AGAAGGGTGT AGCCTATACC 
TTACTGACCC CAAAGGACAG CAATTTTGCT GGTGACCTTG TACGGAACCT GGAAGGAGCC 
AACCAACATG TTTCTAAGGA ACTCTTAGAT CTCGCAATGC AGAACGCCTG GTTTCGGAAG 
TCACGCTTCA AAGGAGGGAA AGGCAAAAAG CTGAACATCG GTGGAGGAGG CTTGGGCTAT 
AGAGAGCGGC CTGGGCTGGG CTCTGAGAAT TCGGACCGAG GAAGTAACAA CAATGTAATG 
AGCAATTATG AAGCCTACAA GCCCTCCACA GGAGCCATGG GAGATAGGCT GACAGCAATG 
AAAGCAGCTT TCCAGTCCCA GTATAAGAGT CACTTTGTTG CAGCCAGTTT AAGTAATCAG 
AAGGCCGGAA CCTCTACTGC TGGGGCAAGT GGATGGACTA GTGCAGGAAG CTTAAATTCT 
GTTCCAACTA ATTCAGCACA GCAGGGCCAT AATAGTCCTG ACAATCCCAT CACCAGTTCT 
ACCAAGAACA TCCCAGGCTT CAACAATTCT GGGAATATCA GTAGTGCCCC AGTGACCTAC 
CCTTCTATTG GAGCCCAAGG AGTCAACAAC ACAGCTTCAG GGAGTAACAG CCGTGAAGGG 
ATTGGGGGTG GCAATGGGAA AAGAGAGAGA TATACTGAGA ACCGGGGTGG TGGCCGTCAT 
AGCCATGGAG ACAGTGGCAA TCGACATGGA GATGGTGGCC GCCATGGAGA TGGATACCGT 
TACCCAGAAA GCAGTAGCCG TCATACGGAT GGCCACCGAC ATGGAGAGAC CAGGCACGGA 
GGAAGTGCAA GCAGACATGG AGAAAGCCGG GGTGCAAATG ATGGCAGGAA TGGAGAAAGC 
AGGAAAGAAG GCTTTAATCG TGAAAACAGG ATGGACCCCA AGGTGGACAG CAGCAGGATG 
GACAAAGTGG ACAGCAAGAC AGATAAGACA CCTGATGGCT TTGCTGTTCC AGAGCCACCC 
AAACGCAAGA AAAGCCGATG GGATAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247659.1
CDS	101..2890
Translation	MNWNKGGPGTKRGFGFGGFAISAGKKEEAKLPQQSHSAFGAASSSSGFGKSAPPQLPSFYKIGSKRANFDEENAYFEDEEEDSSNVDLPYIPAENSPTRQQFRSKPADSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPSKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVILDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENSDRGSNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGTSTAGASGWTSAGSLNSVPTNSAQQGHNSPDNPITSSTKNIPGFNNSGNISSAPVTYPSIGAQGVNNTASGSNSREGIGGGNGKRERYTENRGGGRHSHGDSGNRHGDGGRHGDGYRYPESSSRHTDGHRHGETRHGGSASRHGESRGANDGRNGESRKEGFNRENRMDPKVDSSRMDKVDSKTDKTPDGFAVPEPPKRKKSRWDS

Target ORF information:

RefSeq Version	XM_006247597.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 42 (Ddx42), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247597.3

ORF Insert Sequence:

ATGAATTGGA ATAAAGGTGG ACCTGGAACC AAGCGGGGGT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAGAAGGA GGAAGCCAAG CTGCCGCAGC AGTCGCACAG TGCCTTCGGG 
GCGGCCAGCT CTTCTTCTGG ATTTGGAAAG TCTGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAACGGGC CAACTTTGAT GAAGAAAATG CTTATTTCGA GGATGAAGAA 
GAAGACTCCA GCAATGTAGA CTTACCTTAC ATTCCTGCTG AAAACTCGCC TACCCGCCAG 
CAGTTCCGAT CCAAGCCAGC AGACTCTGAC AGTGACGACG ACCCCTTAGA GGCATTCATG 
GCTGAAGTAG AGGATCAGGC GGCTAGAGAC ATGAAGAGAC TTGAGGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGGGTAGTTC AGGAGGAAGA GGAAGACAAT 
CTGGAGTATG ATAGTGATGG AAATCCAATT GCACCTTCTA AAAAGATTAT CGATCCCCTT 
CCTCCCATTG ATCATTCGGA GATTGACTAT CCACCATTTG AAAAAAATTT TTACAATGAA 
CATGAGGAGA TAACCAACCT CACTCCACAG CAGCTAATAG ACCTGCGGCA TAAACTCAAC 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GTTTTGCTCA TTTTGGGTTT 
GATGAACAAC TTATGCATCA GATTAGGAAA TCTGAGTACA CACAGCCAAC TCCAATACAG 
TGTCAGGGTG TGCCCGTAGC ACTAAGTGGT AGAGACATGA TCGGTATTGC TAAAACAGGC 
AGTGGGAAAA CCGCAGCCTT TATTTGGCCC ATGTTGATTC ACATAATGGA TCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACTAGGGA GCTTTGTCAA 
CAGATCCACG CCGAGTGCAA GCGTTTTGGG AAAGCATATA ATCTTCGATC AGTGGCAGTA 
TATGGAGGAG GGAGCATGTG GGAGCAGGCC AAGGCCCTTC AGGAAGGGGC AGAGATTGTT 
GTGTGTACCC CGGGTCGGCT GATTGATCAT GTGAAGAAAA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCTGAT CGAATGTTCG ACATGGGGTT TGAGTATCAG 
GTTCGGTCCA TAGCAAGTCA TGTCCGTCCT GACAGACAAA CTCTCTTATT CAGTGCAACT 
TTTCGGAAAA AGATTGAAAA ATTGGCCAGA GACATCTTGA TTGATCCTAT CCGAGTGGTA 
CAAGGAGACA TCGGAGAGGC AAATGAAGAC GTAACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTCACTCGG CGTCTGGTAG AGTTTACTTC TTCCGGGAGT 
GTCCTCTTGT TTGTTACCAA GAAAGCCAAC GCTGAAGAAC TGGCCAATAA CCTTAAGCAG 
GAGGGTCATA ATCTTGGTCT GCTCCATGGG GACATGGATC AGAGTGAAAG AAACAAGGTC 
ATTTTAGATT TTAAGAAAAA AGACATCCCC GTCCTGGTGG CCACTGACGT TGCAGCCCGT 
GGTCTGGATA TTCCTTCAAT TAAGACAGTC ATTAACTATG ATGTTGCACG AGACATTGAT 
ACCCATACTC ATAGGATTGG CCGAACAGGA AGAGCTGGTG AGAAGGGTGT AGCCTATACC 
TTACTGACCC CAAAGGACAG CAATTTTGCT GGTGACCTTG TACGGAACCT GGAAGGAGCC 
AACCAACATG TTTCTAAGGA ACTCTTAGAT CTCGCAATGC AGAACGCCTG GTTTCGGAAG 
TCACGCTTCA AAGGAGGGAA AGGCAAAAAG CTGAACATCG GTGGAGGAGG CTTGGGCTAT 
AGAGAGCGGC CTGGGCTGGG CTCTGAGAAT TCGGACCGAG GAAGTAACAA CAATGTAATG 
AGCAATTATG AAGCCTACAA GCCCTCCACA GGAGCCATGG GAGATAGGCT GACAGCAATG 
AAAGCAGCTT TCCAGTCCCA GTATAAGAGT CACTTTGTTG CAGCCAGTTT AAGTAATCAG 
AAGGCCGGAA CCTCTACTGC TGGGGCAAGT GGATGGACTA GTGCAGGAAG CTTAAATTCT 
GTTCCAACTA ATTCAGCACA GCAGGGCCAT AATAGTCCTG ACAATCCCAT CACCAGTTCT 
ACCAAGAACA TCCCAGGCTT CAACAATTCT GGGAATATCA GTAGTGCCCC AGTGACCTAC 
CCTTCTATTG GAGCCCAAGG AGTCAACAAC ACAGCTTCAG GGAGTAACAG CCGTGAAGGG 
ATTGGGGGTG GCAATGGGAA AAGAGAGAGA TATACTGAGA ACCGGGGTGG TGGCCGTCAT 
AGCCATGGAG ACAGTGGCAA TCGACATGGA GATGGTGGCC GCCATGGAGA TGGATACCGT 
TACCCAGAAA GCAGTAGCCG TCATACGGAT GGCCACCGAC ATGGAGAGAC CAGGCACGGA 
GGAAGTGCAA GCAGACATGG AGAAAGCCGG GGTGCAAATG ATGGCAGGAA TGGAGAAAGC 
AGGAAAGAAG GCTTTAATCG TGAAAACAGG ATGGACCCCA AGGTGGACAG CAGCAGGATG 
GACAAAGTGG ACAGCAAGAC AGATAAGACA CCTGATGGCT TTGCTGTTCC AGAGCCACCC 
AAACGCAAGA AAAGCCGATG GGATAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanhäusser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

The DEAD box protein Ddx42p modulates the function of ASPP2, a stimulator of apoptosis.
Oncogene28(20)2065-73(2009 May)
Uhlmann-Schiffler H,Kiermayer S,Stahl H

Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors.
Nucleic acids research35(12)3928-44(2007)
Chen YI,Moore RE,Ge HY,Young MK,Lee TD,Stevens SW

Gene expression profiling of the bone marrow mononuclear cells from patients with myelodysplastic syndrome.
Oncology reports14(5)1189-97(2005 Nov)
Qian J,Chen Z,Wang W,Cen J,Xue Y

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Ddx42 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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