Hacd3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hacd3
Entrez Gene ID	300783
Full Name	3-hydroxyacyl-CoA dehydratase 3
Synonyms	Ptplad1,RGD1565496
General protein information	Preferred Names 3-hydroxyacyl-CoA dehydratase 3 Names very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3 protein tyrosine phosphatase-like A domain containing 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106831.1	NP_001100301.1	very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ptplad1	NP_001038449.1
Xenopus tropicalis (tropical clawed frog)	ptplad1	XP_002938394.2
Pan troglodytes (chimpanzee)	PTPLAD1	XP_001158964.2
Mus musculus (house mouse)	Ptplad1	NP_067320.2
Canis lupus familiaris (dog)	PTPLAD1	XP_535518.2
Rattus norvegicus (Norway rat)	Ptplad1	NP_001100301.1
Drosophila melanogaster (fruit fly)	CG9267	NP_609655.2
Bos taurus (cattle)	PTPLAD1	NP_001096786.1
Homo sapiens (human)	PTPLAD1	NP_057479.2
Macaca mulatta (Rhesus monkey)	PTPLAD1	XP_001109856.1
Gallus gallus (chicken)	PTPLAD1	NP_001007829.1

Gene Ontology
Bibliography

Related articles in PubMed

The last enzyme of the de novo purine synthesis pathway 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) plays a central role in insulin signaling and the Golgi/endosomes protein network.
Boutchueng-Djidjou M, Collard-Simard G, Fortier S, Hébert SS, Kelly I, Landry CR, Faure RL
Molecular & cellular proteomics : MCP14(4)1079-92(2015 Apr)

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Nature cell biology13(4)383-93(2011 Apr)

Characterization of four mammalian 3-hydroxyacyl-CoA dehydratases involved in very long-chain fatty acid synthesis.
Ikeda M, Kanao Y, Yamanaka M, Sakuraba H, Mizutani Y, Igarashi Y, Kihara A
FEBS letters582(16)2435-40(2008 Jul)

Human butyrate-induced transcript 1 interacts with hepatitis C virus NS5A and regulates viral replication.
Taguwa S, Okamoto T, Abe T, Mori Y, Suzuki T, Moriishi K, Matsuura Y
Journal of virology82(6)2631-41(2008 Mar)

B-ind1, a novel mediator of Rac1 signaling cloned from sodium butyrate-treated fibroblasts.
Courilleau D, Chastre E, Sabbah M, Redeuilh G, Atfi A, Mester J
The Journal of biological chemistry275(23)17344-8(2000 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hacd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hacd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02301	NM_001106831.1 Latest version!	Rattus norvegicus 3-hydroxyacyl-CoA dehydratase 3 (Hacd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hacd3 ( NM_001106831.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100301.1 Rattus norvegicus 3-hydroxyacyl-CoA dehydratase 3 (Hacd3), mRNA.

Price & Availability↑

CloneID	ORa02301
Clone ID Related Accession (Same CDS sequence)	NM_001106831.1
Accession Version	NM_001106831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527350400000
Organism	Rattus norvegicus(Norway rat)
Product	very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473975.1. On or before Oct 4, 2007 this sequence version replaced XM_217180.4, XM_001076292.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGACTC AAGTGCTGAC GCCGCACGTC TACTGGGCTC AGCGACACCG CGAGCTGTAT 
CTGCGCGTGG AGCTGAGTGA CGTGCAGAAC CCTGCTATCA GCATCTCAGA AAATGTGCTG 
CATTTCAAAG CTCAGGGACA CGGTGCCAAA GGAGACAATG TCTATGAATT TCACCTGGAG 
TTCTTAGACC TCGTGAAGCC AGAGCCGGCA TACAGGCTGA CCCAGAGGCA GGTGAACATC 
ACAGTCCAGA AGAAGGGGAG TCATTGGTGG GAGAGGCTCA CCAAGCAGGA GAAGCGCCCA 
CTGTTTTTGG CCCCTGACTT TGATCGCTGG CTGGATGAAT CTGATGCAGA AATGGAGCTG 
AGAGCCAAGG AGGAAGAACG CCTAAATAAA CTCAGACTAG AAAGCGAAGG CTCCCCTGAA 
ACTCTTACCA ACTTGAAGAA AGGGTACCTG TTCATGTACA ACCTCGTGCA GCTCCTGGGC 
TTCTCCTGGA TCTTTGTCAA CCTCACAGTG CGGTTCTTTA TTTTGGGAAA AGAGTCCTTC 
TATGACACGT TCCACACCGT GGCTGACATG ATGTATTTCT GCCAGATGCT GGCACTAGTG 
GAAACCCTCA ATGCCGCAAT TGGGGTAACG AATTCCCCGG TGCTGCCTGC TCTCATCCAG 
TTTCTTGGAA GAAATTTTAT TCTGTTTCTC GTTTTCGGCA CCATGGAAGA AATGCAGAAC 
AAAGCCGTGG TTTTCTTTGT GTTTTACTCA TGGAGCGCAA TTGAAATTTT CAGGTACCCC 
TTCTACATGC TGTCCTGTAT TGACATGGAC TGGAAGGTGC TTACGTGGCT TCGTTACACT 
ATGTGGATTC CCTTATATCC CCTGGGATGT CTGTCAGAAG CTGTCGCAGT GATCCAGTCT 
ATTCCAGTAT TCAACGAGTC AGGAAGGTTC AGTTTTACTT TGCCATATCC ATTGAGGATG 
AAAGTCAGAT TCTCCTTCTT CCTTCAAGTT TATCTTGTAA TGTTATTTTT AGGGTTATAT 
ATTAACTTCC GTCACCTTTA TAAACAGCGC AGACGGCGGT ATGGACAAAA AAAGAAAAAA 
CTCCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100301.1
CDS	202..1290
Translation	METQVLTPHVYWAQRHRELYLRVELSDVQNPAISISENVLHFKAQGHGAKGDNVYEFHLEFLDLVKPEPAYRLTQRQVNITVQKKGSHWWERLTKQEKRPLFLAPDFDRWLDESDAEMELRAKEEERLNKLRLESEGSPETLTNLKKGYLFMYNLVQLLGFSWIFVNLTVRFFILGKESFYDTFHTVADMMYFCQMLALVETLNAAIGVTNSPVLPALIQFLGRNFILFLVFGTMEEMQNKAVVFFVFYSWSAIEIFRYPFYMLSCIDMDWKVLTWLRYTMWIPLYPLGCLSEAVAVIQSIPVFNESGRFSFTLPYPLRMKVRFSFFLQVYLVMLFLGLYINFRHLYKQRRRRYGQKKKKLH

Target ORF information:

RefSeq Version	NM_001106831.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 3-hydroxyacyl-CoA dehydratase 3 (Hacd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106831.1

ORF Insert Sequence:

ATGGAGACTC AAGTGCTGAC GCCGCACGTC TACTGGGCTC AGCGACACCG CGAGCTGTAT 
CTGCGCGTGG AGCTGAGTGA CGTGCAGAAC CCTGCTATCA GCATCTCAGA AAATGTGCTG 
CATTTCAAAG CTCAGGGACA CGGTGCCAAA GGAGACAATG TCTATGAATT TCACCTGGAG 
TTCTTAGACC TCGTGAAGCC AGAGCCGGCA TACAGGCTGA CCCAGAGGCA GGTGAACATC 
ACAGTCCAGA AGAAGGGGAG TCATTGGTGG GAGAGGCTCA CCAAGCAGGA GAAGCGCCCA 
CTGTTTTTGG CCCCTGACTT TGATCGCTGG CTGGATGAAT CTGATGCAGA AATGGAGCTG 
AGAGCCAAGG AGGAAGAACG CCTAAATAAA CTCAGACTAG AAAGCGAAGG CTCCCCTGAA 
ACTCTTACCA ACTTGAAGAA AGGGTACCTG TTCATGTACA ACCTCGTGCA GCTCCTGGGC 
TTCTCCTGGA TCTTTGTCAA CCTCACAGTG CGGTTCTTTA TTTTGGGAAA AGAGTCCTTC 
TATGACACGT TCCACACCGT GGCTGACATG ATGTATTTCT GCCAGATGCT GGCACTAGTG 
GAAACCCTCA ATGCCGCAAT TGGGGTAACG AATTCCCCGG TGCTGCCTGC TCTCATCCAG 
TTTCTTGGAA GAAATTTTAT TCTGTTTCTC GTTTTCGGCA CCATGGAAGA AATGCAGAAC 
AAAGCCGTGG TTTTCTTTGT GTTTTACTCA TGGAGCGCAA TTGAAATTTT CAGGTACCCC 
TTCTACATGC TGTCCTGTAT TGACATGGAC TGGAAGGTGC TTACGTGGCT TCGTTACACT 
ATGTGGATTC CCTTATATCC CCTGGGATGT CTGTCAGAAG CTGTCGCAGT GATCCAGTCT 
ATTCCAGTAT TCAACGAGTC AGGAAGGTTC AGTTTTACTT TGCCATATCC ATTGAGGATG 
AAAGTCAGAT TCTCCTTCTT CCTTCAAGTT TATCTTGTAA TGTTATTTTT AGGGTTATAT 
ATTAACTTCC GTCACCTTTA TAAACAGCGC AGACGGCGGT ATGGACAAAA AAAGAAAAAA 
CTCCACTAA