Ecsit cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ecsit
Entrez Gene ID	300447
Full Name	ECSIT signalling integrator
General protein information	Preferred Names ECSIT signalling integrator Names evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial ECSIT homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q13

mRNA and Protein(s)

mRNA	Protein	Name
XM_006242636.3	XP_006242698.1	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform X1
XM_017595531.1	XP_017451020.1	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform X3
XM_017595532.1	XP_017451021.1	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform X4
XM_006242637.3	XP_006242699.1	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform X1
NM_001006986.1	NP_001006987.1	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial precursor
XM_006242638.3	XP_006242700.1	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005295	XP_309087.4
Homo sapiens (human)	ECSIT	NP_057665.2
Pan troglodytes (chimpanzee)	ECSIT	XP_512396.2
Macaca mulatta (Rhesus monkey)	LOC714805	XP_001106441.1
Canis lupus familiaris (dog)	ECSIT	XP_542054.2
Mus musculus (house mouse)	Ecsit	NP_001240826.1
Danio rerio (zebrafish)	ecsit	NP_001229867.1
Caenorhabditis elegans (roundworm)	Y17G9B.5	NP_500631.1
Xenopus tropicalis (tropical clawed frog)	ecsit	NP_001072411.1
Bos taurus (cattle)	ECSIT	NP_001029458.1
Rattus norvegicus (Norway rat)	Ecsit	NP_001006987.1
Drosophila melanogaster (fruit fly)	ECSIT	NP_649602.2
Gallus gallus (chicken)	ECSIT	XP_003643592.1

Gene Ontology
Bibliography

Related articles in PubMed

TRIM59 interacts with ECSIT and negatively regulates NF-κB and IRF-3/7-mediated signal pathways.
Kondo T, Watanabe M, Hatakeyama S
Biochemical and biophysical research communications422(3)501-7(2012 Jun)

Cytosolic signaling protein Ecsit also localizes to mitochondria where it interacts with chaperone NDUFAF1 and functions in complex I assembly.
Vogel RO, Janssen RJ, van den Brand MA, Dieteren CE, Verkaart S, Koopman WJ, Willems PH, Pluk W, van den Heuvel LP, Smeitink JA, Nijtmans LG
Genes & development21(5)615-24(2007 Mar)

Ecsit is required for Bmp signaling and mesoderm formation during mouse embryogenesis.
Xiao C, Shim JH, Klüppel M, Zhang SS, Dong C, Flavell RA, Fu XY, Wrana JL, Hogan BL, Ghosh S
Genes & development17(23)2933-49(2003 Dec)

ECSIT is an evolutionarily conserved intermediate in the Toll/IL-1 signal transduction pathway.
Kopp E, Medzhitov R, Carothers J, Xiao C, Douglas I, Janeway CA, Ghosh S
Genes & development13(16)2059-71(1999 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ecsit gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ecsit cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12485	NM_001006986.1 Latest version!	Rattus norvegicus ECSIT signaling integrator (Ecsit), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa12485	XM_006242638.3 Latest version!	Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa30909	XM_006242637.3 Latest version!	Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa30909	XM_006242636.3 Latest version!	Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa62359	XM_017595531.1 Latest version!	Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa62360	XM_017595532.1 Latest version!	Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ecsit ( NM_001006986.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001006987.1 Rattus norvegicus ECSIT signaling integrator (Ecsit), mRNA.

Price & Availability↑

CloneID	ORa12485
Clone ID Related Accession (Same CDS sequence)	NM_001006986.1 , XM_006242638.3
Accession Version	NM_001006986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Rattus norvegicus(Norway rat)
Product	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083762.1. On Nov 22, 2004 this sequence version replaced XM_217092.2. ##Evidence-Data-START## Transcript exon combination :: BC083762.1, FQ209658.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGAGCTGGG TACAGGTCAA CTTGCTGGCC CGAGGCCTCT CCAGGGGCTG GGGCAGCATC 
TGCAGGACTG TGCTCTCAGG GACCCCCTTT GCTCAGCCGT CCCTTCAGGC TCGAGGCCTC 
CATTGTAGTG CAGTCACACA CAAGGATGAC GTGTGGTTGG TTCCTCGACC CTCCGAGCCC 
CAAAAGAAAC CCATCAAGGT TCCAGCAATG CACGAAGACC TGTTCAGGCC ATCAGGAAAC 
GGGGAGCAGG ACAAGGCAAG CTTCCTGAAT GCAGTGCGCA GTTTTGGGGA GCACAATGTG 
CGCAAGCGCG GCCACGTGGA CTTCATCTAC CTAGCACTTC GCAAGATGCC AGAGTTTGGT 
GTGGAACGGG ACTTGTCAGT ATACAACCTG CTGCTGGACG TTTTCCCCAA GGAGGTCTTC 
CGGCCCCGCA ACGCTATCCA GCGCATCTTT GTCCACTACC CACGGCAGCA GGAGTGTGGA 
GTCGCTGTCC TGGAGCAGAT GGAACGACAT GGAGTTATGC CCAACACAGA GACTGAGTTC 
CTGCTGATCC AGGTGTTCGG GCACAAAAGT TACCCCATGC TCAAGTTCCT GCGGATGAAG 
CTGTGGTTCA CCCGCTTCAA GAATATCAAC CCCTACCCAG TGCCCCGAGA TCTTCCCCAG 
GACCCTTTGG ACCTGGCCAA ACTAGGCCTG CGACACATGG AGCCTGATCT CAGTGCTAAA 
GTCACTGTCT ACCAGATGTC TTTGCCCAGT GAGTCGACAG GCATTGAAGA TCCCACACAG 
CCTCACATTG TAGGAATCCA GAGTCCAGAT CAGCAAGCTG CCCTGGCCCG ACACAACCCA 
TCCAGGCCTG TTTTTGTTGA GGGCCCCTTC CCTCTCTGGC TTCGTAATAA GTGCGTCTAT 
TATCACATCC TAAGAGCTGA CCTGCCACCC CCTGAGGAAG AGACAGTAGA GGAGATCCCA 
GAAGAATGGA ACCTGTACTA CCCAATGCAG CTGGACCTGG AATATTCAAG GAGTGCTTGG 
GACAACTATG AATTTGACAT GGATGAAGTG ACAGAAGGCC CCGTCTTCGC CATGTGCATG 
ACTGGTGCCC ATGATCAGGC AACGTTGGTC AAGTGGATCC AAGGCTTGCA GGAGACCAAC 
CCAACGCTGG CACAGATCCC AGTGGTATTC CGCCTGGCCA GGTCCACAGG AGAGCTCCTG 
GCAACCACAA GGCTGGAGGG ACAGTCCCCT CCCCACAGCC CTCCCAAGGG TCCTGAGGAA 
GATGATGAGG CCATTCAGGC ACAGCAGCGG CAAGGTCAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006987.1
CDS	75..1379
Translation	MSWVQVNLLARGLSRGWGSICRTVLSGTPFAQPSLQARGLHCSAVTHKDDVWLVPRPSEPQKKPIKVPAMHEDLFRPSGNGEQDKASFLNAVRSFGEHNVRKRGHVDFIYLALRKMPEFGVERDLSVYNLLLDVFPKEVFRPRNAIQRIFVHYPRQQECGVAVLEQMERHGVMPNTETEFLLIQVFGHKSYPMLKFLRMKLWFTRFKNINPYPVPRDLPQDPLDLAKLGLRHMEPDLSAKVTVYQMSLPSESTGIEDPTQPHIVGIQSPDQQAALARHNPSRPVFVEGPFPLWLRNKCVYYHILRADLPPPEEETVEEIPEEWNLYYPMQLDLEYSRSAWDNYEFDMDEVTEGPVFAMCMTGAHDQATLVKWIQGLQETNPTLAQIPVVFRLARSTGELLATTRLEGQSPPHSPPKGPEEDDEAIQAQQRQGQS

Target ORF information:

RefSeq Version	NM_001006986.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ECSIT signaling integrator (Ecsit), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006986.1

ORF Insert Sequence:

ATGAGCTGGG TACAGGTCAA CTTGCTGGCC CGAGGCCTCT CCAGGGGCTG GGGCAGCATC 
TGCAGGACTG TGCTCTCAGG GACCCCCTTT GCTCAGCCGT CCCTTCAGGC TCGAGGCCTC 
CATTGTAGTG CAGTCACACA CAAGGATGAC GTGTGGTTGG TTCCTCGACC CTCCGAGCCC 
CAAAAGAAAC CCATCAAGGT TCCAGCAATG CACGAAGACC TGTTCAGGCC ATCAGGAAAC 
GGGGAGCAGG ACAAGGCAAG CTTCCTGAAT GCAGTGCGCA GTTTTGGGGA GCACAATGTG 
CGCAAGCGCG GCCACGTGGA CTTCATCTAC CTAGCACTTC GCAAGATGCC AGAGTTTGGT 
GTGGAACGGG ACTTGTCAGT ATACAACCTG CTGCTGGACG TTTTCCCCAA GGAGGTCTTC 
CGGCCCCGCA ACGCTATCCA GCGCATCTTT GTCCACTACC CACGGCAGCA GGAGTGTGGA 
GTCGCTGTCC TGGAGCAGAT GGAACGACAT GGAGTTATGC CCAACACAGA GACTGAGTTC 
CTGCTGATCC AGGTGTTCGG GCACAAAAGT TACCCCATGC TCAAGTTCCT GCGGATGAAG 
CTGTGGTTCA CCCGCTTCAA GAATATCAAC CCCTACCCAG TGCCCCGAGA TCTTCCCCAG 
GACCCTTTGG ACCTGGCCAA ACTAGGCCTG CGACACATGG AGCCTGATCT CAGTGCTAAA 
GTCACTGTCT ACCAGATGTC TTTGCCCAGT GAGTCGACAG GCATTGAAGA TCCCACACAG 
CCTCACATTG TAGGAATCCA GAGTCCAGAT CAGCAAGCTG CCCTGGCCCG ACACAACCCA 
TCCAGGCCTG TTTTTGTTGA GGGCCCCTTC CCTCTCTGGC TTCGTAATAA GTGCGTCTAT 
TATCACATCC TAAGAGCTGA CCTGCCACCC CCTGAGGAAG AGACAGTAGA GGAGATCCCA 
GAAGAATGGA ACCTGTACTA CCCAATGCAG CTGGACCTGG AATATTCAAG GAGTGCTTGG 
GACAACTATG AATTTGACAT GGATGAAGTG ACAGAAGGCC CCGTCTTCGC CATGTGCATG 
ACTGGTGCCC ATGATCAGGC AACGTTGGTC AAGTGGATCC AAGGCTTGCA GGAGACCAAC 
CCAACGCTGG CACAGATCCC AGTGGTATTC CGCCTGGCCA GGTCCACAGG AGAGCTCCTG 
GCAACCACAA GGCTGGAGGG ACAGTCCCCT CCCCACAGCC CTCCCAAGGG TCCTGAGGAA 
GATGATGAGG CCATTCAGGC ACAGCAGCGG CAAGGTCAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ecsit ( XM_006242638.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242700.1 Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa12485
Clone ID Related Accession (Same CDS sequence)	NM_001006986.1 , XM_006242638.3
Accession Version	XM_006242638.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242638.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG TACAGGTCAA CTTGCTGGCC CGAGGCCTCT CCAGGGGCTG GGGCAGCATC 
TGCAGGACTG TGCTCTCAGG GACCCCCTTT GCTCAGCCGT CCCTTCAGGC TCGAGGCCTC 
CATTGTAGTG CAGTCACACA CAAGGATGAC GTGTGGTTGG TTCCTCGACC CTCCGAGCCC 
CAAAAGAAAC CCATCAAGGT TCCAGCAATG CACGAAGACC TGTTCAGGCC ATCAGGAAAC 
GGGGAGCAGG ACAAGGCAAG CTTCCTGAAT GCAGTGCGCA GTTTTGGGGA GCACAATGTG 
CGCAAGCGCG GCCACGTGGA CTTCATCTAC CTAGCACTTC GCAAGATGCC AGAGTTTGGT 
GTGGAACGGG ACTTGTCAGT ATACAACCTG CTGCTGGACG TTTTCCCCAA GGAGGTCTTC 
CGGCCCCGCA ACGCTATCCA GCGCATCTTT GTCCACTACC CACGGCAGCA GGAGTGTGGA 
GTCGCTGTCC TGGAGCAGAT GGAACGACAT GGAGTTATGC CCAACACAGA GACTGAGTTC 
CTGCTGATCC AGGTGTTCGG GCACAAAAGT TACCCCATGC TCAAGTTCCT GCGGATGAAG 
CTGTGGTTCA CCCGCTTCAA GAATATCAAC CCCTACCCAG TGCCCCGAGA TCTTCCCCAG 
GACCCTTTGG ACCTGGCCAA ACTAGGCCTG CGACACATGG AGCCTGATCT CAGTGCTAAA 
GTCACTGTCT ACCAGATGTC TTTGCCCAGT GAGTCGACAG GCATTGAAGA TCCCACACAG 
CCTCACATTG TAGGAATCCA GAGTCCAGAT CAGCAAGCTG CCCTGGCCCG ACACAACCCA 
TCCAGGCCTG TTTTTGTTGA GGGCCCCTTC CCTCTCTGGC TTCGTAATAA GTGCGTCTAT 
TATCACATCC TAAGAGCTGA CCTGCCACCC CCTGAGGAAG AGACAGTAGA GGAGATCCCA 
GAAGAATGGA ACCTGTACTA CCCAATGCAG CTGGACCTGG AATATTCAAG GAGTGCTTGG 
GACAACTATG AATTTGACAT GGATGAAGTG ACAGAAGGCC CCGTCTTCGC CATGTGCATG 
ACTGGTGCCC ATGATCAGGC AACGTTGGTC AAGTGGATCC AAGGCTTGCA GGAGACCAAC 
CCAACGCTGG CACAGATCCC AGTGGTATTC CGCCTGGCCA GGTCCACAGG AGAGCTCCTG 
GCAACCACAA GGCTGGAGGG ACAGTCCCCT CCCCACAGCC CTCCCAAGGG TCCTGAGGAA 
GATGATGAGG CCATTCAGGC ACAGCAGCGG CAAGGTCAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242700.1
CDS	114..1418
Translation	MSWVQVNLLARGLSRGWGSICRTVLSGTPFAQPSLQARGLHCSAVTHKDDVWLVPRPSEPQKKPIKVPAMHEDLFRPSGNGEQDKASFLNAVRSFGEHNVRKRGHVDFIYLALRKMPEFGVERDLSVYNLLLDVFPKEVFRPRNAIQRIFVHYPRQQECGVAVLEQMERHGVMPNTETEFLLIQVFGHKSYPMLKFLRMKLWFTRFKNINPYPVPRDLPQDPLDLAKLGLRHMEPDLSAKVTVYQMSLPSESTGIEDPTQPHIVGIQSPDQQAALARHNPSRPVFVEGPFPLWLRNKCVYYHILRADLPPPEEETVEEIPEEWNLYYPMQLDLEYSRSAWDNYEFDMDEVTEGPVFAMCMTGAHDQATLVKWIQGLQETNPTLAQIPVVFRLARSTGELLATTRLEGQSPPHSPPKGPEEDDEAIQAQQRQGQS

Target ORF information:

RefSeq Version	XM_006242638.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242638.3

ORF Insert Sequence:

ATGAGCTGGG TACAGGTCAA CTTGCTGGCC CGAGGCCTCT CCAGGGGCTG GGGCAGCATC 
TGCAGGACTG TGCTCTCAGG GACCCCCTTT GCTCAGCCGT CCCTTCAGGC TCGAGGCCTC 
CATTGTAGTG CAGTCACACA CAAGGATGAC GTGTGGTTGG TTCCTCGACC CTCCGAGCCC 
CAAAAGAAAC CCATCAAGGT TCCAGCAATG CACGAAGACC TGTTCAGGCC ATCAGGAAAC 
GGGGAGCAGG ACAAGGCAAG CTTCCTGAAT GCAGTGCGCA GTTTTGGGGA GCACAATGTG 
CGCAAGCGCG GCCACGTGGA CTTCATCTAC CTAGCACTTC GCAAGATGCC AGAGTTTGGT 
GTGGAACGGG ACTTGTCAGT ATACAACCTG CTGCTGGACG TTTTCCCCAA GGAGGTCTTC 
CGGCCCCGCA ACGCTATCCA GCGCATCTTT GTCCACTACC CACGGCAGCA GGAGTGTGGA 
GTCGCTGTCC TGGAGCAGAT GGAACGACAT GGAGTTATGC CCAACACAGA GACTGAGTTC 
CTGCTGATCC AGGTGTTCGG GCACAAAAGT TACCCCATGC TCAAGTTCCT GCGGATGAAG 
CTGTGGTTCA CCCGCTTCAA GAATATCAAC CCCTACCCAG TGCCCCGAGA TCTTCCCCAG 
GACCCTTTGG ACCTGGCCAA ACTAGGCCTG CGACACATGG AGCCTGATCT CAGTGCTAAA 
GTCACTGTCT ACCAGATGTC TTTGCCCAGT GAGTCGACAG GCATTGAAGA TCCCACACAG 
CCTCACATTG TAGGAATCCA GAGTCCAGAT CAGCAAGCTG CCCTGGCCCG ACACAACCCA 
TCCAGGCCTG TTTTTGTTGA GGGCCCCTTC CCTCTCTGGC TTCGTAATAA GTGCGTCTAT 
TATCACATCC TAAGAGCTGA CCTGCCACCC CCTGAGGAAG AGACAGTAGA GGAGATCCCA 
GAAGAATGGA ACCTGTACTA CCCAATGCAG CTGGACCTGG AATATTCAAG GAGTGCTTGG 
GACAACTATG AATTTGACAT GGATGAAGTG ACAGAAGGCC CCGTCTTCGC CATGTGCATG 
ACTGGTGCCC ATGATCAGGC AACGTTGGTC AAGTGGATCC AAGGCTTGCA GGAGACCAAC 
CCAACGCTGG CACAGATCCC AGTGGTATTC CGCCTGGCCA GGTCCACAGG AGAGCTCCTG 
GCAACCACAA GGCTGGAGGG ACAGTCCCCT CCCCACAGCC CTCCCAAGGG TCCTGAGGAA 
GATGATGAGG CCATTCAGGC ACAGCAGCGG CAAGGTCAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ecsit ( XM_006242636.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242698.1 Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa30909
Clone ID Related Accession (Same CDS sequence)	XM_006242636.3 , XM_006242637.3
Accession Version	XM_006242636.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242636.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG TACAGGTCAA CTTGCTGGCC CGAGGCCTCT CCAGGGGCTG GGGCAGCATC 
TGCAGGACTG TGCTCTCAGG GACCCCCTTT GCTCAGCCGT CCCTTCAGGC TCGAGGCCTC 
CATTGTAGTG CAGTCACACA CAAGGATGAC GTGTGGTTGG TTCCTCGACC CTCCGAGCCC 
CAAAAGAAAC CCATCAAGGT TCCAGCAATG CACGAAGACC TGTTCAGGCC ATCAGGAAAC 
GGGGAGCAGG ACAAGGCAAG CTTCCTGAAT GCAGTGCGCA GTTTTGGGGA GCACAATGTG 
CGCAAGCGCG GCCACGTGGA CTTCATCTAC CTAGCACTTC GCAAGATGCC AGAGTTTGGT 
GTGGAACGGG ACTTGTCAGT ATACAACCTG CTGCTGGACG TTTTCCCCAA GGAGGTCTTC 
CGGCCCCGCA ACGCTATCCA GCGCATCTTT GTCCACTACC CACGGCAGCA GGAGTGTGGA 
GTCGCTGTCC TGGAGCAGAT GGAACGACAT GGCTGTGCCT CCAAAACAGG AGTTATGCCC 
AACACAGAGA CTGAGTTCCT GCTGATCCAG GTGTTCGGGC ACAAAAGTTA CCCCATGCTC 
AAGTTCCTGC GGATGAAGCT GTGGTTCACC CGCTTCAAGA ATATCAACCC CTACCCAGTG 
CCCCGAGATC TTCCCCAGGA CCCTTTGGAC CTGGCCAAAC TAGGCCTGCG ACACATGGAG 
CCTGATCTCA GTGCTAAAGT CACTGTCTAC CAGATGTCTT TGCCCAGTGA GTCGACAGGC 
ATTGAAGATC CCACACAGCC TCACATTGTA GGAATCCAGA GTCCAGATCA GCAAGCTGCC 
CTGGCCCGAC ACAACCCATC CAGGCCTGTT TTTGTTGAGG GCCCCTTCCC TCTCTGGCTT 
CGTAATAAGT GCGTCTATTA TCACATCCTA AGAGCTGACC TGCCACCCCC TGAGGAAGAG 
ACAGTAGAGG AGATCCCAGA AGAATGGAAC CTGTACTACC CAATGCAGCT GGACCTGGAA 
TATTCAAGGA GTGCTTGGGA CAACTATGAA TTTGACATGG ATGAAGTGAC AGAAGGCCCC 
GTCTTCGCCA TGTGCATGAC TGGTGCCCAT GATCAGGCAA CGTTGGTCAA GTGGATCCAA 
GGCTTGCAGG AGACCAACCC AACGCTGGCA CAGATCCCAG TGGTATTCCG CCTGGCCAGG 
TCCACAGGAG AGCTCCTGGC AACCACAAGG CTGGAGGGAC AGTCCCCTCC CCACAGCCCT 
CCCAAGGGTC CTGAGGAAGA TGATGAGGCC ATTCAGGCAC AGCAGCGGCA AGGTCAAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242698.1
CDS	124..1446
Translation	MSWVQVNLLARGLSRGWGSICRTVLSGTPFAQPSLQARGLHCSAVTHKDDVWLVPRPSEPQKKPIKVPAMHEDLFRPSGNGEQDKASFLNAVRSFGEHNVRKRGHVDFIYLALRKMPEFGVERDLSVYNLLLDVFPKEVFRPRNAIQRIFVHYPRQQECGVAVLEQMERHGCASKTGVMPNTETEFLLIQVFGHKSYPMLKFLRMKLWFTRFKNINPYPVPRDLPQDPLDLAKLGLRHMEPDLSAKVTVYQMSLPSESTGIEDPTQPHIVGIQSPDQQAALARHNPSRPVFVEGPFPLWLRNKCVYYHILRADLPPPEEETVEEIPEEWNLYYPMQLDLEYSRSAWDNYEFDMDEVTEGPVFAMCMTGAHDQATLVKWIQGLQETNPTLAQIPVVFRLARSTGELLATTRLEGQSPPHSPPKGPEEDDEAIQAQQRQGQS

Target ORF information:

RefSeq Version	XM_006242636.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242636.3

ORF Insert Sequence:

ATGAGCTGGG TACAGGTCAA CTTGCTGGCC CGAGGCCTCT CCAGGGGCTG GGGCAGCATC 
TGCAGGACTG TGCTCTCAGG GACCCCCTTT GCTCAGCCGT CCCTTCAGGC TCGAGGCCTC 
CATTGTAGTG CAGTCACACA CAAGGATGAC GTGTGGTTGG TTCCTCGACC CTCCGAGCCC 
CAAAAGAAAC CCATCAAGGT TCCAGCAATG CACGAAGACC TGTTCAGGCC ATCAGGAAAC 
GGGGAGCAGG ACAAGGCAAG CTTCCTGAAT GCAGTGCGCA GTTTTGGGGA GCACAATGTG 
CGCAAGCGCG GCCACGTGGA CTTCATCTAC CTAGCACTTC GCAAGATGCC AGAGTTTGGT 
GTGGAACGGG ACTTGTCAGT ATACAACCTG CTGCTGGACG TTTTCCCCAA GGAGGTCTTC 
CGGCCCCGCA ACGCTATCCA GCGCATCTTT GTCCACTACC CACGGCAGCA GGAGTGTGGA 
GTCGCTGTCC TGGAGCAGAT GGAACGACAT GGCTGTGCCT CCAAAACAGG AGTTATGCCC 
AACACAGAGA CTGAGTTCCT GCTGATCCAG GTGTTCGGGC ACAAAAGTTA CCCCATGCTC 
AAGTTCCTGC GGATGAAGCT GTGGTTCACC CGCTTCAAGA ATATCAACCC CTACCCAGTG 
CCCCGAGATC TTCCCCAGGA CCCTTTGGAC CTGGCCAAAC TAGGCCTGCG ACACATGGAG 
CCTGATCTCA GTGCTAAAGT CACTGTCTAC CAGATGTCTT TGCCCAGTGA GTCGACAGGC 
ATTGAAGATC CCACACAGCC TCACATTGTA GGAATCCAGA GTCCAGATCA GCAAGCTGCC 
CTGGCCCGAC ACAACCCATC CAGGCCTGTT TTTGTTGAGG GCCCCTTCCC TCTCTGGCTT 
CGTAATAAGT GCGTCTATTA TCACATCCTA AGAGCTGACC TGCCACCCCC TGAGGAAGAG 
ACAGTAGAGG AGATCCCAGA AGAATGGAAC CTGTACTACC CAATGCAGCT GGACCTGGAA 
TATTCAAGGA GTGCTTGGGA CAACTATGAA TTTGACATGG ATGAAGTGAC AGAAGGCCCC 
GTCTTCGCCA TGTGCATGAC TGGTGCCCAT GATCAGGCAA CGTTGGTCAA GTGGATCCAA 
GGCTTGCAGG AGACCAACCC AACGCTGGCA CAGATCCCAG TGGTATTCCG CCTGGCCAGG 
TCCACAGGAG AGCTCCTGGC AACCACAAGG CTGGAGGGAC AGTCCCCTCC CCACAGCCCT 
CCCAAGGGTC CTGAGGAAGA TGATGAGGCC ATTCAGGCAC AGCAGCGGCA AGGTCAAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ecsit ( XM_006242637.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242699.1 Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa30909
Clone ID Related Accession (Same CDS sequence)	XM_006242636.3 , XM_006242637.3
Accession Version	XM_006242637.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242637.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG TACAGGTCAA CTTGCTGGCC CGAGGCCTCT CCAGGGGCTG GGGCAGCATC 
TGCAGGACTG TGCTCTCAGG GACCCCCTTT GCTCAGCCGT CCCTTCAGGC TCGAGGCCTC 
CATTGTAGTG CAGTCACACA CAAGGATGAC GTGTGGTTGG TTCCTCGACC CTCCGAGCCC 
CAAAAGAAAC CCATCAAGGT TCCAGCAATG CACGAAGACC TGTTCAGGCC ATCAGGAAAC 
GGGGAGCAGG ACAAGGCAAG CTTCCTGAAT GCAGTGCGCA GTTTTGGGGA GCACAATGTG 
CGCAAGCGCG GCCACGTGGA CTTCATCTAC CTAGCACTTC GCAAGATGCC AGAGTTTGGT 
GTGGAACGGG ACTTGTCAGT ATACAACCTG CTGCTGGACG TTTTCCCCAA GGAGGTCTTC 
CGGCCCCGCA ACGCTATCCA GCGCATCTTT GTCCACTACC CACGGCAGCA GGAGTGTGGA 
GTCGCTGTCC TGGAGCAGAT GGAACGACAT GGCTGTGCCT CCAAAACAGG AGTTATGCCC 
AACACAGAGA CTGAGTTCCT GCTGATCCAG GTGTTCGGGC ACAAAAGTTA CCCCATGCTC 
AAGTTCCTGC GGATGAAGCT GTGGTTCACC CGCTTCAAGA ATATCAACCC CTACCCAGTG 
CCCCGAGATC TTCCCCAGGA CCCTTTGGAC CTGGCCAAAC TAGGCCTGCG ACACATGGAG 
CCTGATCTCA GTGCTAAAGT CACTGTCTAC CAGATGTCTT TGCCCAGTGA GTCGACAGGC 
ATTGAAGATC CCACACAGCC TCACATTGTA GGAATCCAGA GTCCAGATCA GCAAGCTGCC 
CTGGCCCGAC ACAACCCATC CAGGCCTGTT TTTGTTGAGG GCCCCTTCCC TCTCTGGCTT 
CGTAATAAGT GCGTCTATTA TCACATCCTA AGAGCTGACC TGCCACCCCC TGAGGAAGAG 
ACAGTAGAGG AGATCCCAGA AGAATGGAAC CTGTACTACC CAATGCAGCT GGACCTGGAA 
TATTCAAGGA GTGCTTGGGA CAACTATGAA TTTGACATGG ATGAAGTGAC AGAAGGCCCC 
GTCTTCGCCA TGTGCATGAC TGGTGCCCAT GATCAGGCAA CGTTGGTCAA GTGGATCCAA 
GGCTTGCAGG AGACCAACCC AACGCTGGCA CAGATCCCAG TGGTATTCCG CCTGGCCAGG 
TCCACAGGAG AGCTCCTGGC AACCACAAGG CTGGAGGGAC AGTCCCCTCC CCACAGCCCT 
CCCAAGGGTC CTGAGGAAGA TGATGAGGCC ATTCAGGCAC AGCAGCGGCA AGGTCAAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242699.1
CDS	114..1436
Translation	MSWVQVNLLARGLSRGWGSICRTVLSGTPFAQPSLQARGLHCSAVTHKDDVWLVPRPSEPQKKPIKVPAMHEDLFRPSGNGEQDKASFLNAVRSFGEHNVRKRGHVDFIYLALRKMPEFGVERDLSVYNLLLDVFPKEVFRPRNAIQRIFVHYPRQQECGVAVLEQMERHGCASKTGVMPNTETEFLLIQVFGHKSYPMLKFLRMKLWFTRFKNINPYPVPRDLPQDPLDLAKLGLRHMEPDLSAKVTVYQMSLPSESTGIEDPTQPHIVGIQSPDQQAALARHNPSRPVFVEGPFPLWLRNKCVYYHILRADLPPPEEETVEEIPEEWNLYYPMQLDLEYSRSAWDNYEFDMDEVTEGPVFAMCMTGAHDQATLVKWIQGLQETNPTLAQIPVVFRLARSTGELLATTRLEGQSPPHSPPKGPEEDDEAIQAQQRQGQS

Target ORF information:

RefSeq Version	XM_006242637.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242637.3

ORF Insert Sequence:

ATGAGCTGGG TACAGGTCAA CTTGCTGGCC CGAGGCCTCT CCAGGGGCTG GGGCAGCATC 
TGCAGGACTG TGCTCTCAGG GACCCCCTTT GCTCAGCCGT CCCTTCAGGC TCGAGGCCTC 
CATTGTAGTG CAGTCACACA CAAGGATGAC GTGTGGTTGG TTCCTCGACC CTCCGAGCCC 
CAAAAGAAAC CCATCAAGGT TCCAGCAATG CACGAAGACC TGTTCAGGCC ATCAGGAAAC 
GGGGAGCAGG ACAAGGCAAG CTTCCTGAAT GCAGTGCGCA GTTTTGGGGA GCACAATGTG 
CGCAAGCGCG GCCACGTGGA CTTCATCTAC CTAGCACTTC GCAAGATGCC AGAGTTTGGT 
GTGGAACGGG ACTTGTCAGT ATACAACCTG CTGCTGGACG TTTTCCCCAA GGAGGTCTTC 
CGGCCCCGCA ACGCTATCCA GCGCATCTTT GTCCACTACC CACGGCAGCA GGAGTGTGGA 
GTCGCTGTCC TGGAGCAGAT GGAACGACAT GGCTGTGCCT CCAAAACAGG AGTTATGCCC 
AACACAGAGA CTGAGTTCCT GCTGATCCAG GTGTTCGGGC ACAAAAGTTA CCCCATGCTC 
AAGTTCCTGC GGATGAAGCT GTGGTTCACC CGCTTCAAGA ATATCAACCC CTACCCAGTG 
CCCCGAGATC TTCCCCAGGA CCCTTTGGAC CTGGCCAAAC TAGGCCTGCG ACACATGGAG 
CCTGATCTCA GTGCTAAAGT CACTGTCTAC CAGATGTCTT TGCCCAGTGA GTCGACAGGC 
ATTGAAGATC CCACACAGCC TCACATTGTA GGAATCCAGA GTCCAGATCA GCAAGCTGCC 
CTGGCCCGAC ACAACCCATC CAGGCCTGTT TTTGTTGAGG GCCCCTTCCC TCTCTGGCTT 
CGTAATAAGT GCGTCTATTA TCACATCCTA AGAGCTGACC TGCCACCCCC TGAGGAAGAG 
ACAGTAGAGG AGATCCCAGA AGAATGGAAC CTGTACTACC CAATGCAGCT GGACCTGGAA 
TATTCAAGGA GTGCTTGGGA CAACTATGAA TTTGACATGG ATGAAGTGAC AGAAGGCCCC 
GTCTTCGCCA TGTGCATGAC TGGTGCCCAT GATCAGGCAA CGTTGGTCAA GTGGATCCAA 
GGCTTGCAGG AGACCAACCC AACGCTGGCA CAGATCCCAG TGGTATTCCG CCTGGCCAGG 
TCCACAGGAG AGCTCCTGGC AACCACAAGG CTGGAGGGAC AGTCCCCTCC CCACAGCCCT 
CCCAAGGGTC CTGAGGAAGA TGATGAGGCC ATTCAGGCAC AGCAGCGGCA AGGTCAAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ecsit ( XM_017595531.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451020.1 Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa62359
Clone ID Related Accession (Same CDS sequence)	XM_017595531.1
Accession Version	XM_017595531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGAAG ACCTGTTCAG GCCATCAGGA AACGGGGAGC AGGACAAGGC AAGCTTCCTG 
AATGCAGTGC GCAGTTTTGG GGAGCACAAT GTGCGCAAGC GCGGCCACGT GGACTTCATC 
TACCTAGCAC TTCGCAAGAT GCCAGAGTTT GGTGTGGAAC GGGACTTGTC AGTATACAAC 
CTGCTGCTGG ACGTTTTCCC CAAGGAGGTC TTCCGGCCCC GCAACGCTAT CCAGCGCATC 
TTTGTCCACT ACCCACGGCA GCAGGAGTGT GGAGTCGCTG TCCTGGAGCA GATGGAACGA 
CATGGCTGTG CCTCCAAAAC AGGAGTTATG CCCAACACAG AGACTGAGTT CCTGCTGATC 
CAGGTGTTCG GGCACAAAAG TTACCCCATG CTCAAGTTCC TGCGGATGAA GCTGTGGTTC 
ACCCGCTTCA AGAATATCAA CCCCTACCCA GTGCCCCGAG ATCTTCCCCA GGACCCTTTG 
GACCTGGCCA AACTAGGCCT GCGACACATG GAGCCTGATC TCAGTGCTAA AGTCACTGTC 
TACCAGATGT CTTTGCCCAG TGAGTCGACA GGCATTGAAG ATCCCACACA GCCTCACATT 
GTAGGAATCC AGAGTCCAGA TCAGCAAGCT GCCCTGGCCC GACACAACCC ATCCAGGCCT 
GTTTTTGTTG AGGGCCCCTT CCCTCTCTGG CTTCGTAATA AGTGCGTCTA TTATCACATC 
CTAAGAGCTG ACCTGCCACC CCCTGAGGAA GAGACAGTAG AGGAGATCCC AGAAGAATGG 
AACCTGTACT ACCCAATGCA GCTGGACCTG GAATATTCAA GGAGTGCTTG GGACAACTAT 
GAATTTGACA TGGATGAAGT GACAGAAGGC CCCGTCTTCG CCATGTGCAT GACTGGTGCC 
CATGATCAGG CAACGTTGGT CAAGTGGATC CAAGGCTTGC AGGAGACCAA CCCAACGCTG 
GCACAGATCC CAGTGGTATT CCGCCTGGCC AGGTCCACAG GAGAGCTCCT GGCAACCACA 
AGGCTGGAGG GACAGTCCCC TCCCCACAGC CCTCCCAAGG GTCCTGAGGA AGATGATGAG 
GCCATTCAGG CACAGCAGCG GCAAGGTCAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451020.1
CDS	204..1319
Translation	MHEDLFRPSGNGEQDKASFLNAVRSFGEHNVRKRGHVDFIYLALRKMPEFGVERDLSVYNLLLDVFPKEVFRPRNAIQRIFVHYPRQQECGVAVLEQMERHGCASKTGVMPNTETEFLLIQVFGHKSYPMLKFLRMKLWFTRFKNINPYPVPRDLPQDPLDLAKLGLRHMEPDLSAKVTVYQMSLPSESTGIEDPTQPHIVGIQSPDQQAALARHNPSRPVFVEGPFPLWLRNKCVYYHILRADLPPPEEETVEEIPEEWNLYYPMQLDLEYSRSAWDNYEFDMDEVTEGPVFAMCMTGAHDQATLVKWIQGLQETNPTLAQIPVVFRLARSTGELLATTRLEGQSPPHSPPKGPEEDDEAIQAQQRQGQS

Target ORF information:

RefSeq Version	XM_017595531.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595531.1

ORF Insert Sequence:

ATGCACGAAG ACCTGTTCAG GCCATCAGGA AACGGGGAGC AGGACAAGGC AAGCTTCCTG 
AATGCAGTGC GCAGTTTTGG GGAGCACAAT GTGCGCAAGC GCGGCCACGT GGACTTCATC 
TACCTAGCAC TTCGCAAGAT GCCAGAGTTT GGTGTGGAAC GGGACTTGTC AGTATACAAC 
CTGCTGCTGG ACGTTTTCCC CAAGGAGGTC TTCCGGCCCC GCAACGCTAT CCAGCGCATC 
TTTGTCCACT ACCCACGGCA GCAGGAGTGT GGAGTCGCTG TCCTGGAGCA GATGGAACGA 
CATGGCTGTG CCTCCAAAAC AGGAGTTATG CCCAACACAG AGACTGAGTT CCTGCTGATC 
CAGGTGTTCG GGCACAAAAG TTACCCCATG CTCAAGTTCC TGCGGATGAA GCTGTGGTTC 
ACCCGCTTCA AGAATATCAA CCCCTACCCA GTGCCCCGAG ATCTTCCCCA GGACCCTTTG 
GACCTGGCCA AACTAGGCCT GCGACACATG GAGCCTGATC TCAGTGCTAA AGTCACTGTC 
TACCAGATGT CTTTGCCCAG TGAGTCGACA GGCATTGAAG ATCCCACACA GCCTCACATT 
GTAGGAATCC AGAGTCCAGA TCAGCAAGCT GCCCTGGCCC GACACAACCC ATCCAGGCCT 
GTTTTTGTTG AGGGCCCCTT CCCTCTCTGG CTTCGTAATA AGTGCGTCTA TTATCACATC 
CTAAGAGCTG ACCTGCCACC CCCTGAGGAA GAGACAGTAG AGGAGATCCC AGAAGAATGG 
AACCTGTACT ACCCAATGCA GCTGGACCTG GAATATTCAA GGAGTGCTTG GGACAACTAT 
GAATTTGACA TGGATGAAGT GACAGAAGGC CCCGTCTTCG CCATGTGCAT GACTGGTGCC 
CATGATCAGG CAACGTTGGT CAAGTGGATC CAAGGCTTGC AGGAGACCAA CCCAACGCTG 
GCACAGATCC CAGTGGTATT CCGCCTGGCC AGGTCCACAG GAGAGCTCCT GGCAACCACA 
AGGCTGGAGG GACAGTCCCC TCCCCACAGC CCTCCCAAGG GTCCTGAGGA AGATGATGAG 
GCCATTCAGG CACAGCAGCG GCAAGGTCAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ecsit ( XM_017595532.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451021.1 Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa62360
Clone ID Related Accession (Same CDS sequence)	XM_017595532.1
Accession Version	XM_017595532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGAAG ACCTGTTCAG GCCATCAGGA AACGGGGAGC AGGACAAGGC AAGCTTCCTG 
AATGCAGTGC GCAGTTTTGG GGAGCACAAT GTGCGCAAGC GCGGCCACGT GGACTTCATC 
TACCTAGCAC TTCGCAAGAT GCCAGAGTTT GGTGTGGAAC GGGACTTGTC AGTATACAAC 
CTGCTGCTGG ACGTTTTCCC CAAGGAGGTC TTCCGGCCCC GCAACGCTAT CCAGCGCATC 
TTTGTCCACT ACCCACGGCA GCAGGAGTGT GGAGTCGCTG TCCTGGAGCA GATGGAACGA 
CATGGAGTTA TGCCCAACAC AGAGACTGAG TTCCTGCTGA TCCAGGTGTT CGGGCACAAA 
AGTTACCCCA TGCTCAAGTT CCTGCGGATG AAGCTGTGGT TCACCCGCTT CAAGAATATC 
AACCCCTACC CAGTGCCCCG AGATCTTCCC CAGGACCCTT TGGACCTGGC CAAACTAGGC 
CTGCGACACA TGGAGCCTGA TCTCAGTGCT AAAGTCACTG TCTACCAGAT GTCTTTGCCC 
AGTGAGTCGA CAGGCATTGA AGATCCCACA CAGCCTCACA TTGTAGGAAT CCAGAGTCCA 
GATCAGCAAG CTGCCCTGGC CCGACACAAC CCATCCAGGC CTGTTTTTGT TGAGGGCCCC 
TTCCCTCTCT GGCTTCGTAA TAAGTGCGTC TATTATCACA TCCTAAGAGC TGACCTGCCA 
CCCCCTGAGG AAGAGACAGT AGAGGAGATC CCAGAAGAAT GGAACCTGTA CTACCCAATG 
CAGCTGGACC TGGAATATTC AAGGAGTGCT TGGGACAACT ATGAATTTGA CATGGATGAA 
GTGACAGAAG GCCCCGTCTT CGCCATGTGC ATGACTGGTG CCCATGATCA GGCAACGTTG 
GTCAAGTGGA TCCAAGGCTT GCAGGAGACC AACCCAACGC TGGCACAGAT CCCAGTGGTA 
TTCCGCCTGG CCAGGTCCAC AGGAGAGCTC CTGGCAACCA CAAGGCTGGA GGGACAGTCC 
CCTCCCCACA GCCCTCCCAA GGGTCCTGAG GAAGATGATG AGGCCATTCA GGCACAGCAG 
CGGCAAGGTC AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451021.1
CDS	202..1299
Translation	MHEDLFRPSGNGEQDKASFLNAVRSFGEHNVRKRGHVDFIYLALRKMPEFGVERDLSVYNLLLDVFPKEVFRPRNAIQRIFVHYPRQQECGVAVLEQMERHGVMPNTETEFLLIQVFGHKSYPMLKFLRMKLWFTRFKNINPYPVPRDLPQDPLDLAKLGLRHMEPDLSAKVTVYQMSLPSESTGIEDPTQPHIVGIQSPDQQAALARHNPSRPVFVEGPFPLWLRNKCVYYHILRADLPPPEEETVEEIPEEWNLYYPMQLDLEYSRSAWDNYEFDMDEVTEGPVFAMCMTGAHDQATLVKWIQGLQETNPTLAQIPVVFRLARSTGELLATTRLEGQSPPHSPPKGPEEDDEAIQAQQRQGQS

Target ORF information:

RefSeq Version	XM_017595532.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ECSIT signalling integrator (Ecsit), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595532.1

ORF Insert Sequence:

ATGCACGAAG ACCTGTTCAG GCCATCAGGA AACGGGGAGC AGGACAAGGC AAGCTTCCTG 
AATGCAGTGC GCAGTTTTGG GGAGCACAAT GTGCGCAAGC GCGGCCACGT GGACTTCATC 
TACCTAGCAC TTCGCAAGAT GCCAGAGTTT GGTGTGGAAC GGGACTTGTC AGTATACAAC 
CTGCTGCTGG ACGTTTTCCC CAAGGAGGTC TTCCGGCCCC GCAACGCTAT CCAGCGCATC 
TTTGTCCACT ACCCACGGCA GCAGGAGTGT GGAGTCGCTG TCCTGGAGCA GATGGAACGA 
CATGGAGTTA TGCCCAACAC AGAGACTGAG TTCCTGCTGA TCCAGGTGTT CGGGCACAAA 
AGTTACCCCA TGCTCAAGTT CCTGCGGATG AAGCTGTGGT TCACCCGCTT CAAGAATATC 
AACCCCTACC CAGTGCCCCG AGATCTTCCC CAGGACCCTT TGGACCTGGC CAAACTAGGC 
CTGCGACACA TGGAGCCTGA TCTCAGTGCT AAAGTCACTG TCTACCAGAT GTCTTTGCCC 
AGTGAGTCGA CAGGCATTGA AGATCCCACA CAGCCTCACA TTGTAGGAAT CCAGAGTCCA 
GATCAGCAAG CTGCCCTGGC CCGACACAAC CCATCCAGGC CTGTTTTTGT TGAGGGCCCC 
TTCCCTCTCT GGCTTCGTAA TAAGTGCGTC TATTATCACA TCCTAAGAGC TGACCTGCCA 
CCCCCTGAGG AAGAGACAGT AGAGGAGATC CCAGAAGAAT GGAACCTGTA CTACCCAATG 
CAGCTGGACC TGGAATATTC AAGGAGTGCT TGGGACAACT ATGAATTTGA CATGGATGAA 
GTGACAGAAG GCCCCGTCTT CGCCATGTGC ATGACTGGTG CCCATGATCA GGCAACGTTG 
GTCAAGTGGA TCCAAGGCTT GCAGGAGACC AACCCAACGC TGGCACAGAT CCCAGTGGTA 
TTCCGCCTGG CCAGGTCCAC AGGAGAGCTC CTGGCAACCA CAAGGCTGGA GGGACAGTCC 
CCTCCCCACA GCCCTCCCAA GGGTCCTGAG GAAGATGATG AGGCCATTCA GGCACAGCAG 
CGGCAAGGTC AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TRIM59 interacts with ECSIT and negatively regulates NF-κB and IRF-3/7-mediated signal pathways.
Biochemical and biophysical research communications422(3)501-7(2012 Jun)
Kondo T,Watanabe M,Hatakeyama S

Cytosolic signaling protein Ecsit also localizes to mitochondria where it interacts with chaperone NDUFAF1 and functions in complex I assembly.
Genes & development21(5)615-24(2007 Mar)
Vogel RO,Janssen RJ,van den Brand MA,Dieteren CE,Verkaart S,Koopman WJ,Willems PH,Pluk W,van den Heuvel LP,Smeitink JA,Nijtmans LG

Ecsit is required for Bmp signaling and mesoderm formation during mouse embryogenesis.
Genes & development17(23)2933-49(2003 Dec)
Xiao C,Shim JH,Klüppel M,Zhang SS,Dong C,Flavell RA,Fu XY,Wrana JL,Hogan BL,Ghosh S

ECSIT is an evolutionarily conserved intermediate in the Toll/IL-1 signal transduction pathway.
Genes & development13(16)2059-71(1999 Aug)
Kopp E,Medzhitov R,Carothers J,Xiao C,Douglas I,Janeway CA,Ghosh S

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Ecsit cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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