Ckmt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ckmt1
Entrez Gene ID	29593
Full Name	creatine kinase, mitochondrial 1
Synonyms	Ckmt1b
General protein information	Preferred Names creatine kinase, mitochondrial 1 Names creatine kinase U-type, mitochondrial U-MtCK acidic-type mitochondrial creatine kinase creatine kinase, mitochondrial 1, ubiquitous creatine kinase, mitochondrial 1B mia-CK ubiquitous mitochondrial creatine kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q35
Summary	ubiquitous mitochondrial creatine kinase isozyme; catalyzes the transfer of phosphate to creatine in energy transduction [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006234830.3	XP_006234892.1	creatine kinase U-type, mitochondrial isoform X1
XM_017591555.1	XP_017447044.1	creatine kinase U-type, mitochondrial isoform X2
NM_001012738.1	NP_001012756.1	creatine kinase U-type, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CKMT1B	NP_066270.1
Pan troglodytes (chimpanzee)	CKMT1B	XP_003314688.1
Pan troglodytes (chimpanzee)	LOC100608872	XP_003314691.1
Homo sapiens (human)	CKMT1A	NP_001015001.1
Macaca mulatta (Rhesus monkey)	LOC712886	XP_002808494.1
Bos taurus (cattle)	CKMT1B	NP_776700.1
Xenopus tropicalis (tropical clawed frog)	ckmt1b	NP_989369.1
Rattus norvegicus (Norway rat)	Ckmt1b	NP_001012756.1
Gallus gallus (chicken)	CKMT1A	NP_989513.1
Danio rerio (zebrafish)	ckmt1	NP_942096.1
Canis lupus familiaris (dog)	CKMT1B	XP_535451.1
Mus musculus (house mouse)	Ckmt1	NP_034027.1

Gene Ontology
Bibliography

Related articles in PubMed

Metabolism and function of mitochondrial cardiolipin.
Ren M, Phoon CK, Schlame M
Progress in lipid research551-16(2014 Jul)

Decreased neuroinflammation and increased brain energy homeostasis following environmental enrichment after mild traumatic brain injury is associated with improvement in cognitive function.
Briones TL, Woods J, Rogozinska M
Acta neuropathologica communications157(2013 Sep)

Effects of β-alanine administration on selected parameters of oxidative stress and phosphoryltransfer network in cerebral cortex and cerebellum of rats.
Gemelli T, de Andrade RB, Rojas DB, Bonorino NF, Mazzola PN, Tortorelli LS, Funchal C, Filho CS, Wannmacher CM
Molecular and cellular biochemistry380(1-2)161-70(2013 Aug)

Expression of leucine-rich repeat kinase 2 (LRRK2) inhibits the processing of uMtCK to induce cell death in a cell culture model system.
Cui J, Yu M, Niu J, Yue Z, Xu Z
Bioscience reports31(5)429-37(2011 Oct)

Effects of creatine and β-guanidinopropionic acid and alterations in creatine transporter and creatine kinases expression in acute seizure and chronic epilepsy models.
Kim DW, Yeo SI, Ryu HJ, Kim JE, Song HK, Kwon OS, Choi SY, Kang TC
BMC neuroscience11141(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ckmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ckmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13086	NM_001012738.1 Latest version!	Rattus norvegicus creatine kinase, mitochondrial 1 (Ckmt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa13086	XM_006234830.3 Latest version!	Rattus norvegicus creatine kinase, mitochondrial 1B (Ckmt1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa60313	XM_017591555.1 Latest version!	Rattus norvegicus creatine kinase, mitochondrial 1B (Ckmt1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ckmt1 ( NM_001012738.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012756.1 Rattus norvegicus creatine kinase, mitochondrial 1 (Ckmt1), mRNA.

Price & Availability↑

CloneID	ORa13086
Clone ID Related Accession (Same CDS sequence)	XM_006234830.3 , NM_001012738.1
Accession Version	NM_001012738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569081600000
Organism	Rattus norvegicus(Norway rat)
Product	creatine kinase U-type, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC091335.1. On or before Mar 26, 2005 this sequence version replaced XM_342505.1, XM_347216.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC091335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCCG GACTCAAGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCGC CGCCGGGATT CTGCTCCGTC CGGAATCTGT ACGAGCGGCC 
ACCGGTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GCTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCC 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCTAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGCAAAATC CGTTCTGGCT ACTTTGATGA GAGGTACGTA 
TTGTCCTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGACGAGA GGTGGAGCGT GTTGTGGTGG ACGCGCTGAG TGGCCTGAAG 
GGCGACCTGG CTGGACGGTA CTACAGGCTC AGTGAGATGA CAGAGGCTGA GCAGCAGCAG 
CTTATTGACG ACCATTTCCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC AGCAGCAGGC 
ATGGCTCGAG ACTGGCCAGA TGCCCGAGGA ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGATACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTACAC GTCAAACTGC CACTGCTAAG CAAAGATAGC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGG CTGCAAAAGC GTGGGACTGG AGGAGTGGAC 
ACGGCTGCCA CAGGCAGTGT CTTTGACATC TCTAATCTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTGGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAAAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACGGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012756.1
CDS	200..1456
Translation	MAGPFSRLLSARPGLKLLALAGAGSLAAGILLRPESVRAATGERRRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEETYEVFAELFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYVLSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKGGNMKRVFERFCRGLKEVEKLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHVKLPLLSKDSRFPKILENLRLQKRGTGGVDTAATGSVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLEKGQDIRIPPPLVHGKH

Target ORF information:

RefSeq Version	NM_001012738.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus creatine kinase, mitochondrial 1 (Ckmt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012738.1

ORF Insert Sequence:

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCCG GACTCAAGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCGC CGCCGGGATT CTGCTCCGTC CGGAATCTGT ACGAGCGGCC 
ACCGGTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GCTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCC 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCTAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGCAAAATC CGTTCTGGCT ACTTTGATGA GAGGTACGTA 
TTGTCCTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGACGAGA GGTGGAGCGT GTTGTGGTGG ACGCGCTGAG TGGCCTGAAG 
GGCGACCTGG CTGGACGGTA CTACAGGCTC AGTGAGATGA CAGAGGCTGA GCAGCAGCAG 
CTTATTGACG ACCATTTCCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC AGCAGCAGGC 
ATGGCTCGAG ACTGGCCAGA TGCCCGAGGA ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGATACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTACAC GTCAAACTGC CACTGCTAAG CAAAGATAGC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGG CTGCAAAAGC GTGGGACTGG AGGAGTGGAC 
ACGGCTGCCA CAGGCAGTGT CTTTGACATC TCTAATCTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTGGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAAAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACGGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ckmt1 ( XM_006234830.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234892.1 Rattus norvegicus creatine kinase, mitochondrial 1B (Ckmt1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa13086
Clone ID Related Accession (Same CDS sequence)	XM_006234830.3 , NM_001012738.1
Accession Version	XM_006234830.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	creatine kinase U-type, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234830.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCCG GACTCAAGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCGC CGCCGGGATT CTGCTCCGTC CGGAATCTGT ACGAGCGGCC 
ACCGGTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GCTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCC 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCTAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGCAAAATC CGTTCTGGCT ACTTTGATGA GAGGTACGTA 
TTGTCCTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGACGAGA GGTGGAGCGT GTTGTGGTGG ACGCGCTGAG TGGCCTGAAG 
GGCGACCTGG CTGGACGGTA CTACAGGCTC AGTGAGATGA CAGAGGCTGA GCAGCAGCAG 
CTTATTGACG ACCATTTCCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC AGCAGCAGGC 
ATGGCTCGAG ACTGGCCAGA TGCCCGAGGA ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGATACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTACAC GTCAAACTGC CACTGCTAAG CAAAGATAGC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGG CTGCAAAAGC GTGGGACTGG AGGAGTGGAC 
ACGGCTGCCA CAGGCAGTGT CTTTGACATC TCTAATCTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTGGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAAAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACGGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234892.1
CDS	153..1409
Translation	MAGPFSRLLSARPGLKLLALAGAGSLAAGILLRPESVRAATGERRRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEETYEVFAELFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYVLSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKGGNMKRVFERFCRGLKEVEKLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHVKLPLLSKDSRFPKILENLRLQKRGTGGVDTAATGSVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLEKGQDIRIPPPLVHGKH

Target ORF information:

RefSeq Version	XM_006234830.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus creatine kinase, mitochondrial 1B (Ckmt1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234830.3

ORF Insert Sequence:

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCCG GACTCAAGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCGC CGCCGGGATT CTGCTCCGTC CGGAATCTGT ACGAGCGGCC 
ACCGGTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GCTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCC 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCTAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGCAAAATC CGTTCTGGCT ACTTTGATGA GAGGTACGTA 
TTGTCCTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGACGAGA GGTGGAGCGT GTTGTGGTGG ACGCGCTGAG TGGCCTGAAG 
GGCGACCTGG CTGGACGGTA CTACAGGCTC AGTGAGATGA CAGAGGCTGA GCAGCAGCAG 
CTTATTGACG ACCATTTCCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC AGCAGCAGGC 
ATGGCTCGAG ACTGGCCAGA TGCCCGAGGA ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGATACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTACAC GTCAAACTGC CACTGCTAAG CAAAGATAGC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGG CTGCAAAAGC GTGGGACTGG AGGAGTGGAC 
ACGGCTGCCA CAGGCAGTGT CTTTGACATC TCTAATCTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTGGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAAAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACGGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ckmt1 ( XM_017591555.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447044.1 Rattus norvegicus creatine kinase, mitochondrial 1B (Ckmt1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60313
Clone ID Related Accession (Same CDS sequence)	XM_017591555.1
Accession Version	XM_017591555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	creatine kinase U-type, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCACA CCACTGACCT TGATGCCAGC AAAATCCGTT CTGGCTACTT TGATGAGAGG 
TACGTATTGT CCTCAAGAGT CAGAACTGGC CGAAGTATCA GGGGACTCAG TCTCCCTCCA 
GCCTGCACTC GGGCAGAGCG ACGAGAGGTG GAGCGTGTTG TGGTGGACGC GCTGAGTGGC 
CTGAAGGGCG ACCTGGCTGG ACGGTACTAC AGGCTCAGTG AGATGACAGA GGCTGAGCAG 
CAGCAGCTTA TTGACGACCA TTTCCTGTTT GATAAACCTG TGTCCCCATT GCTGACAGCA 
GCAGGCATGG CTCGAGACTG GCCAGATGCC CGAGGAATCT GGCACAACAA TGAGAAGAGT 
TTCTTGATCT GGGTGAATGA GGAGGACCAC ACACGGGTCA TCTCTATGGA GAAAGGCGGC 
AACATGAAGA GAGTGTTTGA AAGATTCTGC CGGGGCCTCA AAGAGGTGGA GAAGCTGATC 
CAGGAACGAG GCTGGGAGTT CATGTGGAAT GAGCGTTTAG GATACATCTT GACCTGTCCA 
TCTAACCTGG GCACTGGACT TCGGGCAGGA GTACACGTCA AACTGCCACT GCTAAGCAAA 
GATAGCCGCT TCCCAAAGAT CCTGGAGAAC CTAAGGCTGC AAAAGCGTGG GACTGGAGGA 
GTGGACACGG CTGCCACAGG CAGTGTCTTT GACATCTCTA ATCTGGATCG ACTTGGCAAG 
TCAGAGGTGG AGCTGGTGCA GCTGGTCATC GATGGGGTGA ACTATTTGAT TGACTGTGAA 
CGGCGTCTGG AGAAAGGACA GGATATTCGA ATCCCTCCAC CTCTTGTCCA CGGCAAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447044.1
CDS	150..992
Translation	MKHTTDLDASKIRSGYFDERYVLSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKGGNMKRVFERFCRGLKEVEKLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHVKLPLLSKDSRFPKILENLRLQKRGTGGVDTAATGSVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLEKGQDIRIPPPLVHGKH

Target ORF information:

RefSeq Version	XM_017591555.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus creatine kinase, mitochondrial 1B (Ckmt1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591555.1

ORF Insert Sequence:

ATGAAGCACA CCACTGACCT TGATGCCAGC AAAATCCGTT CTGGCTACTT TGATGAGAGG 
TACGTATTGT CCTCAAGAGT CAGAACTGGC CGAAGTATCA GGGGACTCAG TCTCCCTCCA 
GCCTGCACTC GGGCAGAGCG ACGAGAGGTG GAGCGTGTTG TGGTGGACGC GCTGAGTGGC 
CTGAAGGGCG ACCTGGCTGG ACGGTACTAC AGGCTCAGTG AGATGACAGA GGCTGAGCAG 
CAGCAGCTTA TTGACGACCA TTTCCTGTTT GATAAACCTG TGTCCCCATT GCTGACAGCA 
GCAGGCATGG CTCGAGACTG GCCAGATGCC CGAGGAATCT GGCACAACAA TGAGAAGAGT 
TTCTTGATCT GGGTGAATGA GGAGGACCAC ACACGGGTCA TCTCTATGGA GAAAGGCGGC 
AACATGAAGA GAGTGTTTGA AAGATTCTGC CGGGGCCTCA AAGAGGTGGA GAAGCTGATC 
CAGGAACGAG GCTGGGAGTT CATGTGGAAT GAGCGTTTAG GATACATCTT GACCTGTCCA 
TCTAACCTGG GCACTGGACT TCGGGCAGGA GTACACGTCA AACTGCCACT GCTAAGCAAA 
GATAGCCGCT TCCCAAAGAT CCTGGAGAAC CTAAGGCTGC AAAAGCGTGG GACTGGAGGA 
GTGGACACGG CTGCCACAGG CAGTGTCTTT GACATCTCTA ATCTGGATCG ACTTGGCAAG 
TCAGAGGTGG AGCTGGTGCA GCTGGTCATC GATGGGGTGA ACTATTTGAT TGACTGTGAA 
CGGCGTCTGG AGAAAGGACA GGATATTCGA ATCCCTCCAC CTCTTGTCCA CGGCAAACAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Metabolism and function of mitochondrial cardiolipin.
Progress in lipid research551-16(2014 Jul)
Ren M,Phoon CK,Schlame M

Decreased neuroinflammation and increased brain energy homeostasis following environmental enrichment after mild traumatic brain injury is associated with improvement in cognitive function.
Acta neuropathologica communications157(2013 Sep)
Briones TL,Woods J,Rogozinska M

Effects of β-alanine administration on selected parameters of oxidative stress and phosphoryltransfer network in cerebral cortex and cerebellum of rats.
Molecular and cellular biochemistry380(1-2)161-70(2013 Aug)
Gemelli T,de Andrade RB,Rojas DB,Bonorino NF,Mazzola PN,Tortorelli LS,Funchal C,Filho CS,Wannmacher CM

Expression of leucine-rich repeat kinase 2 (LRRK2) inhibits the processing of uMtCK to induce cell death in a cell culture model system.
Bioscience reports31(5)429-37(2011 Oct)
Cui J,Yu M,Niu J,Yue Z,Xu Z

Effects of creatine and β-guanidinopropionic acid and alterations in creatine transporter and creatine kinases expression in acute seizure and chronic epilepsy models.
BMC neuroscience11141(2010 Oct)
Kim DW,Yeo SI,Ryu HJ,Kim JE,Song HK,Kwon OS,Choi SY,Kang TC

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Ckmt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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