Ndufb8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndufb8
Entrez Gene ID	293991
Full Name	NADH:ubiquinone oxidoreductase subunit B8
General protein information	Preferred Names NADH:ubiquinone oxidoreductase subunit B8 Names NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q54

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106360.2	NP_001099830.1	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002020	XP_321035.4
Macaca mulatta (Rhesus monkey)	NDUFB8	XP_001108340.1
Bos taurus (cattle)	NDUFB8	NP_777092.1
Rattus norvegicus (Norway rat)	Ndufb8	NP_001099830.1
Pan troglodytes (chimpanzee)	NDUFB8	NP_001138336.1
Mus musculus (house mouse)	Ndufb8	NP_080337.1
Homo sapiens (human)	NDUFB8	NP_004995.1
Danio rerio (zebrafish)	ndufb8	NP_957149.1
Drosophila melanogaster (fruit fly)	CG3192	NP_001027048.1
Xenopus tropicalis (tropical clawed frog)	ndufb8	XP_002936399.2
Canis lupus familiaris (dog)	NDUFB8	XP_005637678.1
Gallus gallus (chicken)	NDUFB8	NP_001006502.1

Gene Ontology
Bibliography

Related articles in PubMed

Accessory subunits are integral for assembly and function of human mitochondrial complex I.
Stroud DA, Surgenor EE, Formosa LE, Reljic B, Frazier AE, Dibley MG, Osellame LD, Stait T, Beilharz TH, Thorburn DR, Salim A, Ryan MT
Nature538(7623)123-126(2016 Oct)

Mitochondrial DNA Depletion in Respiratory Chain-Deficient Parkinson Disease Neurons.
Grünewald A, Rygiel KA, Hepplewhite PD, Morris CM, Picard M, Turnbull DM
Annals of neurology79(3)366-78(2016 Mar)

Partial complex I deficiency due to the CNS conditional ablation of Ndufa5 results in a mild chronic encephalopathy but no increase in oxidative damage.
Peralta S, Torraco A, Wenz T, Garcia S, Diaz F, Moraes CT
Human molecular genetics23(6)1399-412(2014 Mar)

Accelerated recovery of renal mitochondrial and tubule homeostasis with SIRT1/PGC-1α activation following ischemia-reperfusion injury.
Funk JA, Schnellmann RG
Toxicology and applied pharmacology273(2)345-54(2013 Dec)

Sepsis-induced cardiac mitochondrial dysfunction involves altered mitochondrial-localization of tyrosine kinase Src and tyrosine phosphatase SHP2.
Zang QS, Martinez B, Yao X, Maass DL, Ma L, Wolf SE, Minei JP
PloS one7(8)e43424(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ndufb8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndufb8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01089	NM_001106360.2 Latest version!	Rattus norvegicus NADH:ubiquinone oxidoreductase subunit B8 (Ndufb8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndufb8 ( NM_001106360.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099830.1 Rattus norvegicus NADH:ubiquinone oxidoreductase subunit B8 (Ndufb8), mRNA.

Price & Availability↑

CloneID	ORa01089
Clone ID Related Accession (Same CDS sequence)	NM_001106360.2
Accession Version	NM_001106360.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542297600000
Organism	Rattus norvegicus(Norway rat)
Product	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166906.1. On May 11, 2008 this sequence version replaced NM_001106360.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC166889.1, CB313895.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCGT CCCGGGCTGC GGCCCTGGGA GTCCGGTGGC TGCAAAGGAC ATCCCGCTGC 
GTGGTGCCAC TTGAGGCACG GAGAGCCTTC CACATGACCA AGGACATGAT GCCGGGGTCA 
TATCCTAGGA CCCCAGAAGA ACGGGCCGCG GCCGCCAAGA AGTATAATAT GCGTGTGGAA 
GACTACGAGC CGTACCCAGA TGATGGCATG GGGTATGGTG ACTACCCGAT GCTCCCTAAC 
CGCTCACAGC ATGAGAGGGA TCCGTGGTAT GAATGGGACC ACCCAGACCT CAGGCTGAAC 
TGGGGTGAAC CGATACACTG GGACCTAGAC ATGTACATCA GGAATCGTGT GGACACATCT 
CCTACACCGG TTTCCTGGGA TGTCATGTGT AGACATCTCT TCGGCTTTGT GGCTTTCATG 
GTTTTCATGT TCTGGGTAGG GGACATGTTC CCTTCCTACC AGCCTGTGGG TCCGAAGCAG 
TACCCGTACA ATAATCTGTA CCTGGAGCGA GGCGGTGATC CTTCCAAAGA ACCTGAGCCC 
GTGGTTCACT ATGAGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099830.1
CDS	11..571
Translation	MAASRAAALGVRWLQRTSRCVVPLEARRAFHMTKDMMPGSYPRTPEERAAAAKKYNMRVEDYEPYPDDGMGYGDYPMLPNRSQHERDPWYEWDHPDLRLNWGEPIHWDLDMYIRNRVDTSPTPVSWDVMCRHLFGFVAFMVFMFWVGDMFPSYQPVGPKQYPYNNLYLERGGDPSKEPEPVVHYEI

Target ORF information:

RefSeq Version	NM_001106360.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NADH:ubiquinone oxidoreductase subunit B8 (Ndufb8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106360.2

ORF Insert Sequence:

ATGGCGGCGT CCCGGGCTGC GGCCCTGGGA GTCCGGTGGC TGCAAAGGAC ATCCCGCTGC 
GTGGTGCCAC TTGAGGCACG GAGAGCCTTC CACATGACCA AGGACATGAT GCCGGGGTCA 
TATCCTAGGA CCCCAGAAGA ACGGGCCGCG GCCGCCAAGA AGTATAATAT GCGTGTGGAA 
GACTACGAGC CGTACCCAGA TGATGGCATG GGGTATGGTG ACTACCCGAT GCTCCCTAAC 
CGCTCACAGC ATGAGAGGGA TCCGTGGTAT GAATGGGACC ACCCAGACCT CAGGCTGAAC 
TGGGGTGAAC CGATACACTG GGACCTAGAC ATGTACATCA GGAATCGTGT GGACACATCT 
CCTACACCGG TTTCCTGGGA TGTCATGTGT AGACATCTCT TCGGCTTTGT GGCTTTCATG 
GTTTTCATGT TCTGGGTAGG GGACATGTTC CCTTCCTACC AGCCTGTGGG TCCGAAGCAG 
TACCCGTACA ATAATCTGTA CCTGGAGCGA GGCGGTGATC CTTCCAAAGA ACCTGAGCCC 
GTGGTTCACT ATGAGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Accessory subunits are integral for assembly and function of human mitochondrial complex I.
Nature538(7623)123-126(2016 Oct)
Stroud DA,Surgenor EE,Formosa LE,Reljic B,Frazier AE,Dibley MG,Osellame LD,Stait T,Beilharz TH,Thorburn DR,Salim A,Ryan MT

Mitochondrial DNA Depletion in Respiratory Chain-Deficient Parkinson Disease Neurons.
Annals of neurology79(3)366-78(2016 Mar)
Grünewald A,Rygiel KA,Hepplewhite PD,Morris CM,Picard M,Turnbull DM

Partial complex I deficiency due to the CNS conditional ablation of Ndufa5 results in a mild chronic encephalopathy but no increase in oxidative damage.
Human molecular genetics23(6)1399-412(2014 Mar)
Peralta S,Torraco A,Wenz T,Garcia S,Diaz F,Moraes CT

Accelerated recovery of renal mitochondrial and tubule homeostasis with SIRT1/PGC-1α activation following ischemia-reperfusion injury.
Toxicology and applied pharmacology273(2)345-54(2013 Dec)
Funk JA,Schnellmann RG

Sepsis-induced cardiac mitochondrial dysfunction involves altered mitochondrial-localization of tyrosine kinase Src and tyrosine phosphatase SHP2.
PloS one7(8)e43424(2012)
Zang QS,Martinez B,Yao X,Maass DL,Ma L,Wolf SE,Minei JP