Sf3b2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sf3b2
Entrez Gene ID	293671
Full Name	splicing factor 3b, subunit 2
Synonyms	SAP145
General protein information	Preferred Names splicing factor 3b, subunit 2 Names splicing factor 3B subunit 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106326.1	NP_001099796.2	splicing factor 3B subunit 2
XM_008760105.2	XP_008758327.1	splicing factor 3B subunit 2 isoform X1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0827300	NP_001048592.1
Caenorhabditis elegans (roundworm)	CELE_W03F9.10	NP_503141.1
Rattus norvegicus (Norway rat)	Sf3b2	NP_001099796.2
Homo sapiens (human)	SF3B2	NP_006833.2
Macaca mulatta (Rhesus monkey)	SF3B2	XP_002799525.1
Canis lupus familiaris (dog)	SF3B2	XP_533224.2
Xenopus tropicalis (tropical clawed frog)	sf3b2	XP_002935399.1
Bos taurus (cattle)	SF3B2	NP_001096741.1
Mus musculus (house mouse)	Sf3b2	NP_084385.2
Drosophila melanogaster (fruit fly)	CG3605	NP_608739.1
Arabidopsis thaliana (thale cress)	AT4G21660	NP_001190789.1
Pan troglodytes (chimpanzee)	SF3B2	XP_508570.2
Danio rerio (zebrafish)	sf3b2	NP_001098747.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006240	XP_316308.4

Gene Ontology
Bibliography

Related articles in PubMed

Structure of the human activated spliceosome in three conformational states.
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell research28(3)307-322(2018 Mar)

Molecular Architecture of SF3b and Structural Consequences of Its Cancer-Related Mutations.
Cretu C, Schmitzová J, Ponce-Salvatierra A, Dybkov O, De Laurentiis EI, Sharma K, Will CL, Urlaub H, Lührmann R, Pena V
Molecular cell64(2)307-319(2016 10)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanhäusser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors.
Chen YI, Moore RE, Ge HY, Young MK, Lee TD, Stevens SW
Nucleic acids research35(12)3928-44(2007)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sf3b2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sf3b2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00943	NM_001106326.1 Latest version!	Rattus norvegicus splicing factor 3b, subunit 2 (Sf3b2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa45141	XM_008760105.2 Latest version!	Rattus norvegicus splicing factor 3b, subunit 2 (Sf3b2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sf3b2 ( NM_001106326.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099796.2 Rattus norvegicus splicing factor 3b, subunit 2 (Sf3b2), mRNA.

Price & Availability↑

CloneID	ORa00943
Clone ID Related Accession (Same CDS sequence)	NM_001106326.1
Accession Version	NM_001106326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570550400000
Organism	Rattus norvegicus(Norway rat)
Product	splicing factor 3B subunit 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473953.1. On or before Oct 4, 2007 this sequence version replaced XM_215182.4, XM_001073034.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGG AGCATCCTGA ACCTCCCAAA GGAGAATTGC AGTTGCCACC GCCTCCCCCT 
CCAGGCCACT ATGGGGCCTG GGCTGCCCAG GAGCTTCAGG CCAAATTGGC AGAGATAGGA 
GCGCCGATCC AGGGGAGTCG CGAGGAGCTA GTAGAGCGGC TGCAGACCTA CACCCGCCAG 
ACTGGCATTG TGCTGAATCG ACCAGTTTTG AGAGGAGAGG ATGGGGACAA AGCTGCTCCT 
CCTCCGATGT CAGCACAGCT CTCTGGGATT CCTATGCCGC CGCCACCTTT GGGACTCCCC 
CCTCTACAGC CTCCTCCACC ACCTCCACCA CCTCCACCAG GCCTTGGCCT TGGCTTTCCT 
ATGGCTCACC CACCAAATCT GGGGCCCCCA CCACCTCTTC GAGTGGGTGA GCCTGTGGCA 
CTGTCAGAAG AAGAGCGGCT GAAGCTGGCC CAGCAGCAGG CAGCTTTACT GATGCAGCAA 
GAGGAACGCG CCAAACAGGC AGCCGTATTA ATGGAGCAGG AACGGCAGCA GGAAATTGCC 
AAGATGGGCA CTGCAGTCCC TAGGCCTCCT CAGGATATGG GGCAGCTGGG GGTCCGTACT 
CCTCTGGGGC CTCGAGTAGC TGCTCCAGTA GGCCCTGTGG TTCCCACACC TACTGTTTTG 
CCCATGGGGG CACCTGTTCC TCGCCCTCGG GGTCCCCCGC CACCCCCTGG AGATGAGAAC 
AGAGAGATGG ATGATCCTTC TGTGGGCCCC AAGATCCCCC AGGCTTTGGA AAAGATTCTG 
CAGCTGAAGG AGAGCCGTCA GGAAGAGATG AACTCCCAGC AGGAGGAAGA AGAAATGGAG 
ACAGACACTC GCTCATCTCT GGGACAGTCA GCATCAGAGA CTGAGGAGGA CACTGTGTCT 
ACATCCAAAA AAGAGAAAAA CCGGAAGCGT CGGAACCGAA AGAAGAAGAA AAAGCCACAG 
CGGGTGCGGG CAGCCTCGTC AGAGAGCTCC GGGGACCGAG AGAAAGACTC AGCTCGGTCC 
CGAGGCTCTG ACTCTCCTGC TGCTGATGTG GAGATTGAGT ATGTGACTGA AGAGCCTGAA 
ATCTACGAGC CCAACTTCAT CTTCTTCAAG AGGATTTTTG AGGCTTTCAA GCTAACTGAT 
GATGTGAAAA AGGAAAAGGA AAAGGAGCCA GAGAAGCTTG ACAAGATGGA GAACTCTGCA 
GTCCCCAAGA AGAAGGGCTT TGAGGAGGAG CACAAAGACA GTGACGATGA CAGCAGTGAC 
GACGAACAGG AGAAGAAGCC GGAAGCCCCT AAGCTGTCCA AGAAGAAGCT GCGCCGGATG 
AACCGCTTCA CCGTGGCAGA GCTGAAGCAG CTGGTGGCCC GACCTGATGT TGTTGAGATG 
CATGACGTGA CAGCACAGGA CCCTAAGCTT TTGGTTCACC TCAAGGCCAC CCGGAACTCT 
GTCCCTGTGC CACGCCACTG GTGTTTTAAG CGCAAGTACC TTCAAGGCAA ACGAGGCATT 
GAGAAACCCC CCTTTGAGCT GCCAGACTTC ATCAAACGCA CAGGCATTCA GGAGATGCGA 
GAGGCCCTGC AGGAGAAGGA AGAACAGAAG ACCATGAAGT CAAAAATGAG AGAGAAGGTT 
CGGCCGAAGA TGGGGAAGAT TGACATTGAC TACCAGAAGT TACACGATGC CTTCTTCAAG 
TGGCAGACCA AGCCGAAGCT CACCATCCAT GGGGACCTTT ATTACGAGGG GAAGGAGTTT 
GAGACACGGC TGAAGGAGAA GAAGCCTGGA GATTTGTCTG ATGAGCTAAG GATTTCTTTA 
GGGATGCCAG TAGGACCTAA TGCCCACAAA GTTCCTCCTC CGTGGCTGAT TGCCATGCAG 
CGATACGGGC CACCGCCATC CTACCCAAAC CTGAAAATCC CTGGACTGAA CTCACCTATC 
CCTGAGAGCT GTTCCTTTGG ATACCATGCT GGTGGCTGGG GCAAACCCCC AGTAGATGAG 
ACTGGGAAGC CACTTTATGG GGACGTGTTT GGAACCAATG CTGCTGAGTT TCAGACCAAA 
ACTGAAGAAG AAGAAATTGA CCGGACTCCA TGGGGGGAGC TGGAGCCATC GGATGAAGAA 
TCCTCAGAAG AGGAGGAAGA GGAGGAAAGC GATGAAGACA AGCCAGATGA GACTGGCTTT 
ATCACACCTG CGGACAGTGG CCTCATCACT CCTGGAGGGT TCTCCTCCGT GCCAGCTGGA 
ATGGAGACCC CTGAACTCAT TGAGCTGAGA AAGAAGAAGA TTGAAGAGGC GATGGATGGG 
AGCGAGACAC CTCAGTTGTT CACTGTATTG CCAGAGAAGA GGACAGCCAC TGTTGGTGGG 
GCCATGATGG GATCTACCCA CATTTATGAC ATGTCCACGG TTATGAGCCG GAAGGGCCCG 
GCCCCTGAGT TGCAAGGTGT GGAAGTAGCA CTGGCTCCCG AGGAGTTGGA GTTGGACCCC 
ATGGCCATGA CCCAGAAGTA TGAGGAGCAT GTCCGGGAGC AGCAGGCCCA AGTCGAGAAA 
GAAGACTTCA GTGACATGGT GGCGGAGCAT GCTGCCAAAC AGAAGCAAAA GAAACGGAAA 
GCTCAGCCCC AGGACAGTCG TGGGGGCAGC AAGAAATACA AGGAGTTCAA ATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099796.2
CDS	55..2691
Translation	MAAEHPEPPKGELQLPPPPPPGHYGAWAAQELQAKLAEIGAPIQGSREELVERLQTYTRQTGIVLNRPVLRGEDGDKAAPPPMSAQLSGIPMPPPPLGLPPLQPPPPPPPPPPGLGLGFPMAHPPNLGPPPPLRVGEPVALSEEERLKLAQQQAALLMQQEERAKQAAVLMEQERQQEIAKMGTAVPRPPQDMGQLGVRTPLGPRVAAPVGPVVPTPTVLPMGAPVPRPRGPPPPPGDENREMDDPSVGPKIPQALEKILQLKESRQEEMNSQQEEEEMETDTRSSLGQSASETEEDTVSTSKKEKNRKRRNRKKKKKPQRVRAASSESSGDREKDSARSRGSDSPAADVEIEYVTEEPEIYEPNFIFFKRIFEAFKLTDDVKKEKEKEPEKLDKMENSAVPKKKGFEEEHKDSDDDSSDDEQEKKPEAPKLSKKKLRRMNRFTVAELKQLVARPDVVEMHDVTAQDPKLLVHLKATRNSVPVPRHWCFKRKYLQGKRGIEKPPFELPDFIKRTGIQEMREALQEKEEQKTMKSKMREKVRPKMGKIDIDYQKLHDAFFKWQTKPKLTIHGDLYYEGKEFETRLKEKKPGDLSDELRISLGMPVGPNAHKVPPPWLIAMQRYGPPPSYPNLKIPGLNSPIPESCSFGYHAGGWGKPPVDETGKPLYGDVFGTNAAEFQTKTEEEEIDRTPWGELEPSDEESSEEEEEEESDEDKPDETGFITPADSGLITPGGFSSVPAGMETPELIELRKKKIEEAMDGSETPQLFTVLPEKRTATVGGAMMGSTHIYDMSTVMSRKGPAPELQGVEVALAPEELELDPMAMTQKYEEHVREQQAQVEKEDFSDMVAEHAAKQKQKKRKAQPQDSRGGSKKYKEFKF

Target ORF information:

RefSeq Version	NM_001106326.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus splicing factor 3b, subunit 2 (Sf3b2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106326.1

ORF Insert Sequence:

ATGGCGGCGG AGCATCCTGA ACCTCCCAAA GGAGAATTGC AGTTGCCACC GCCTCCCCCT 
CCAGGCCACT ATGGGGCCTG GGCTGCCCAG GAGCTTCAGG CCAAATTGGC AGAGATAGGA 
GCGCCGATCC AGGGGAGTCG CGAGGAGCTA GTAGAGCGGC TGCAGACCTA CACCCGCCAG 
ACTGGCATTG TGCTGAATCG ACCAGTTTTG AGAGGAGAGG ATGGGGACAA AGCTGCTCCT 
CCTCCGATGT CAGCACAGCT CTCTGGGATT CCTATGCCGC CGCCACCTTT GGGACTCCCC 
CCTCTACAGC CTCCTCCACC ACCTCCACCA CCTCCACCAG GCCTTGGCCT TGGCTTTCCT 
ATGGCTCACC CACCAAATCT GGGGCCCCCA CCACCTCTTC GAGTGGGTGA GCCTGTGGCA 
CTGTCAGAAG AAGAGCGGCT GAAGCTGGCC CAGCAGCAGG CAGCTTTACT GATGCAGCAA 
GAGGAACGCG CCAAACAGGC AGCCGTATTA ATGGAGCAGG AACGGCAGCA GGAAATTGCC 
AAGATGGGCA CTGCAGTCCC TAGGCCTCCT CAGGATATGG GGCAGCTGGG GGTCCGTACT 
CCTCTGGGGC CTCGAGTAGC TGCTCCAGTA GGCCCTGTGG TTCCCACACC TACTGTTTTG 
CCCATGGGGG CACCTGTTCC TCGCCCTCGG GGTCCCCCGC CACCCCCTGG AGATGAGAAC 
AGAGAGATGG ATGATCCTTC TGTGGGCCCC AAGATCCCCC AGGCTTTGGA AAAGATTCTG 
CAGCTGAAGG AGAGCCGTCA GGAAGAGATG AACTCCCAGC AGGAGGAAGA AGAAATGGAG 
ACAGACACTC GCTCATCTCT GGGACAGTCA GCATCAGAGA CTGAGGAGGA CACTGTGTCT 
ACATCCAAAA AAGAGAAAAA CCGGAAGCGT CGGAACCGAA AGAAGAAGAA AAAGCCACAG 
CGGGTGCGGG CAGCCTCGTC AGAGAGCTCC GGGGACCGAG AGAAAGACTC AGCTCGGTCC 
CGAGGCTCTG ACTCTCCTGC TGCTGATGTG GAGATTGAGT ATGTGACTGA AGAGCCTGAA 
ATCTACGAGC CCAACTTCAT CTTCTTCAAG AGGATTTTTG AGGCTTTCAA GCTAACTGAT 
GATGTGAAAA AGGAAAAGGA AAAGGAGCCA GAGAAGCTTG ACAAGATGGA GAACTCTGCA 
GTCCCCAAGA AGAAGGGCTT TGAGGAGGAG CACAAAGACA GTGACGATGA CAGCAGTGAC 
GACGAACAGG AGAAGAAGCC GGAAGCCCCT AAGCTGTCCA AGAAGAAGCT GCGCCGGATG 
AACCGCTTCA CCGTGGCAGA GCTGAAGCAG CTGGTGGCCC GACCTGATGT TGTTGAGATG 
CATGACGTGA CAGCACAGGA CCCTAAGCTT TTGGTTCACC TCAAGGCCAC CCGGAACTCT 
GTCCCTGTGC CACGCCACTG GTGTTTTAAG CGCAAGTACC TTCAAGGCAA ACGAGGCATT 
GAGAAACCCC CCTTTGAGCT GCCAGACTTC ATCAAACGCA CAGGCATTCA GGAGATGCGA 
GAGGCCCTGC AGGAGAAGGA AGAACAGAAG ACCATGAAGT CAAAAATGAG AGAGAAGGTT 
CGGCCGAAGA TGGGGAAGAT TGACATTGAC TACCAGAAGT TACACGATGC CTTCTTCAAG 
TGGCAGACCA AGCCGAAGCT CACCATCCAT GGGGACCTTT ATTACGAGGG GAAGGAGTTT 
GAGACACGGC TGAAGGAGAA GAAGCCTGGA GATTTGTCTG ATGAGCTAAG GATTTCTTTA 
GGGATGCCAG TAGGACCTAA TGCCCACAAA GTTCCTCCTC CGTGGCTGAT TGCCATGCAG 
CGATACGGGC CACCGCCATC CTACCCAAAC CTGAAAATCC CTGGACTGAA CTCACCTATC 
CCTGAGAGCT GTTCCTTTGG ATACCATGCT GGTGGCTGGG GCAAACCCCC AGTAGATGAG 
ACTGGGAAGC CACTTTATGG GGACGTGTTT GGAACCAATG CTGCTGAGTT TCAGACCAAA 
ACTGAAGAAG AAGAAATTGA CCGGACTCCA TGGGGGGAGC TGGAGCCATC GGATGAAGAA 
TCCTCAGAAG AGGAGGAAGA GGAGGAAAGC GATGAAGACA AGCCAGATGA GACTGGCTTT 
ATCACACCTG CGGACAGTGG CCTCATCACT CCTGGAGGGT TCTCCTCCGT GCCAGCTGGA 
ATGGAGACCC CTGAACTCAT TGAGCTGAGA AAGAAGAAGA TTGAAGAGGC GATGGATGGG 
AGCGAGACAC CTCAGTTGTT CACTGTATTG CCAGAGAAGA GGACAGCCAC TGTTGGTGGG 
GCCATGATGG GATCTACCCA CATTTATGAC ATGTCCACGG TTATGAGCCG GAAGGGCCCG 
GCCCCTGAGT TGCAAGGTGT GGAAGTAGCA CTGGCTCCCG AGGAGTTGGA GTTGGACCCC 
ATGGCCATGA CCCAGAAGTA TGAGGAGCAT GTCCGGGAGC AGCAGGCCCA AGTCGAGAAA 
GAAGACTTCA GTGACATGGT GGCGGAGCAT GCTGCCAAAC AGAAGCAAAA GAAACGGAAA 
GCTCAGCCCC AGGACAGTCG TGGGGGCAGC AAGAAATACA AGGAGTTCAA ATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sf3b2 ( XM_008760105.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758327.1 Rattus norvegicus splicing factor 3b, subunit 2 (Sf3b2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa45141
Clone ID Related Accession (Same CDS sequence)	XM_008760105.2
Accession Version	XM_008760105.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	splicing factor 3B subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760105.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCATCCTGA ACCTCCCAAA GGAGAATTGC AGTTGCCACC GCCTCCCCCT 
CCAGGCCACT ATGGGGCCTG GGCTGCCCAG GAGCTTCAGG CCAAATTGGC AGAGATAGGA 
GCGCCGATCC AGGGGAGTCG CGAGGAGCTA GTAGAGCGGC TGCAGACCTA CACCCGCCAG 
ACTGGCATTG TGCTGAATCG ACCAGTTTTG AGAGGAGAGG ATGGGGACAA AGCTGCTCCT 
CCTCCGATGT CAGCACAGCT CTCTGGGATT CCTATGCCGC CGCCACCTTT GGGACTCCCC 
CCTCTACAGC CTCCTCCACC ACCTCCACCA CCTCCACCAG GCCTTGGCCT TGGCTTTCCT 
ATGGCTCACC CACCAAATCT GGGGCCCCCA CCACCTCTTC GAGTGGGTGA GCCTGTGGCA 
CTGTCAGAAG AAGAGCGGCT GAAGCTGGCC CAGCAGCAGG CAGCTTTACT GATGCAGCAA 
GAGGAACGCG CCAAACAGGC AGCCGTATTA ATGGAGCAGG AACGGCAGCA GGAAATTGCC 
AAGATGGGCA CTGCAGTCCC TAGGCCTCCT CAGGATATGG GGCAGCTGGG GGTCCGTACT 
CCTCTGGGGC CTCGAGCTGC TCCAGTAGGC CCTGTGGTTC CCACACCTAC TGTTTTGCCC 
ATGGGGGCAC CTGTTCCTCG CCCTCGGGGT CCCCCGCCAC CCCCTGGAGA TGAGAACAGA 
GAGATGGATG ATCCTTCTGT GGGCCCCAAG ATCCCCCAGG CTTTGGAAAA GATTCTGCAG 
CTGAAGGAGA GCCGTCAGGA AGAGATGAAC TCCCAGCAGG AGGAAGAAGA AATGGAGACA 
GACACTCGCT CATCTCTGGG ACAGTCAGCA TCAGAGACTG AGGAGGACAC TGTGTCTACA 
TCCAAAAAAG AGAAAAACCG GAAGCGTCGG AACCGAAAGA AGAAGAAAAA GCCACAGCGG 
GTGCGGGCAG CCTCGTCAGA GAGCTCCGGG GACCGAGAGA AAGACTCAGC TCGGTCCCGA 
GGCTCTGACT CTCCTGCTGC TGATGTGGAG ATTGAGTATG TGACTGAAGA GCCTGAAATC 
TACGAGCCCA ACTTCATCTT CTTCAAGAGG ATTTTTGAGG CTTTCAAGCT AACTGATGAT 
GTGAAAAAGG AAAAGGAAAA GGAGCCAGAG AAGCTTGACA AGATGGAGAA CTCTGCAGTC 
CCCAAGAAGA AGGGCTTTGA GGAGGAGCAC AAAGACAGTG ACGATGACAG CAGTGACGAC 
GAACAGGAGA AGAAGCCGGA AGCCCCTAAG CTGTCCAAGA AGAAGCTGCG CCGGATGAAC 
CGCTTCACCG TGGCAGAGCT GAAGCAGCTG GTGGCCCGAC CTGATGTTGT TGAGATGCAT 
GACGTGACAG CACAGGACCC TAAGCTTTTG GTTCACCTCA AGGCCACCCG GAACTCTGTC 
CCTGTGCCAC GCCACTGGTG TTTTAAGCGC AAGTACCTTC AAGGCAAACG AGGCATTGAG 
AAACCCCCCT TTGAGCTGCC AGACTTCATC AAACGCACAG GCATTCAGGA GATGCGAGAG 
GCCCTGCAGG AGAAGGAAGA ACAGAAGACC ATGAAGTCAA AAATGAGAGA GAAGGTTCGG 
CCGAAGATGG GGAAGATTGA CATTGACTAC CAGAAGTTAC ACGATGCCTT CTTCAAGTGG 
CAGACCAAGC CGAAGCTCAC CATCCATGGG GACCTTTATT ACGAGGGGAA GGAGTTTGAG 
ACACGGCTGA AGGAGAAGAA GCCTGGAGAT TTGTCTGATG AGCTAAGGAT TTCTTTAGGG 
ATGCCAGTAG GACCTAATGC CCACAAAGTT CCTCCTCCGT GGCTGATTGC CATGCAGCGA 
TACGGGCCAC CGCCATCCTA CCCAAACCTG AAAATCCCTG GACTGAACTC ACCTATCCCT 
GAGAGCTGTC CTCTTGGATA CCATGCTGGT GGCTGGGGCA AACCCCCAGT AGATGAGACT 
GGGAAGCCAC TTTATGGGGA CGTGTTTGGA ACCAATGCTG CTGAGTTTCA GACCAAAACT 
GAAGAAGAAG AAATTGACCG GACTCCATGG GGGGAGCTGG AGCCATCGGA TGAAGAATCC 
TCAGAAGAGG AGGAAGAGGA GGAAAGCGAT GAAGACAAGC CAGATGAGAC TGGCTTTATC 
ACACCTGCGG ACAGTGGCCT CATCACTCCT GGAGGGTTCT CCTCCGTGCC AGCTGGAATG 
GAGACCCCTG AACTCATTGA GCTGAGAAAG AAGAAGATTG AAGAGGCGAT GGATGGGAGC 
GAGACACCTC AGTTGTTCAC TGTATTGCCA GAGAAGAGGA CAGCCACTGT TGGTGGGGCC 
ATGATGGGAT CTACCCACAT TTATGACATG TCCACGGTTA TGAGCCGGAA GGGCCCGGCC 
CCTGAGTTGC AAGGTGTGGA AGTAGCACTG GCTCCCGAGG AGTTGGAGTT GGACCCCATG 
GCCATGACCC AGAAGTATGA GGAGCATGTC CGGGAGCAGC AGGCCCAAGT CGAGAAAGAA 
GACTTCAGTG ACATGGTGGC GGAGCATGCT GCCAAACAGA AGCAAAAGAA ACGGAAAGCT 
CAGCCCCAGG ACAGTCGTGG GGGCAGCAAG AAATACAAGG AGTTCAAATT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758327.1
CDS	48..2681
Translation	MAAEHPEPPKGELQLPPPPPPGHYGAWAAQELQAKLAEIGAPIQGSREELVERLQTYTRQTGIVLNRPVLRGEDGDKAAPPPMSAQLSGIPMPPPPLGLPPLQPPPPPPPPPPGLGLGFPMAHPPNLGPPPPLRVGEPVALSEEERLKLAQQQAALLMQQEERAKQAAVLMEQERQQEIAKMGTAVPRPPQDMGQLGVRTPLGPRAAPVGPVVPTPTVLPMGAPVPRPRGPPPPPGDENREMDDPSVGPKIPQALEKILQLKESRQEEMNSQQEEEEMETDTRSSLGQSASETEEDTVSTSKKEKNRKRRNRKKKKKPQRVRAASSESSGDREKDSARSRGSDSPAADVEIEYVTEEPEIYEPNFIFFKRIFEAFKLTDDVKKEKEKEPEKLDKMENSAVPKKKGFEEEHKDSDDDSSDDEQEKKPEAPKLSKKKLRRMNRFTVAELKQLVARPDVVEMHDVTAQDPKLLVHLKATRNSVPVPRHWCFKRKYLQGKRGIEKPPFELPDFIKRTGIQEMREALQEKEEQKTMKSKMREKVRPKMGKIDIDYQKLHDAFFKWQTKPKLTIHGDLYYEGKEFETRLKEKKPGDLSDELRISLGMPVGPNAHKVPPPWLIAMQRYGPPPSYPNLKIPGLNSPIPESCPLGYHAGGWGKPPVDETGKPLYGDVFGTNAAEFQTKTEEEEIDRTPWGELEPSDEESSEEEEEEESDEDKPDETGFITPADSGLITPGGFSSVPAGMETPELIELRKKKIEEAMDGSETPQLFTVLPEKRTATVGGAMMGSTHIYDMSTVMSRKGPAPELQGVEVALAPEELELDPMAMTQKYEEHVREQQAQVEKEDFSDMVAEHAAKQKQKKRKAQPQDSRGGSKKYKEFKF

Target ORF information:

RefSeq Version	XM_008760105.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus splicing factor 3b, subunit 2 (Sf3b2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760105.2

ORF Insert Sequence:

ATGGCGGCGG AGCATCCTGA ACCTCCCAAA GGAGAATTGC AGTTGCCACC GCCTCCCCCT 
CCAGGCCACT ATGGGGCCTG GGCTGCCCAG GAGCTTCAGG CCAAATTGGC AGAGATAGGA 
GCGCCGATCC AGGGGAGTCG CGAGGAGCTA GTAGAGCGGC TGCAGACCTA CACCCGCCAG 
ACTGGCATTG TGCTGAATCG ACCAGTTTTG AGAGGAGAGG ATGGGGACAA AGCTGCTCCT 
CCTCCGATGT CAGCACAGCT CTCTGGGATT CCTATGCCGC CGCCACCTTT GGGACTCCCC 
CCTCTACAGC CTCCTCCACC ACCTCCACCA CCTCCACCAG GCCTTGGCCT TGGCTTTCCT 
ATGGCTCACC CACCAAATCT GGGGCCCCCA CCACCTCTTC GAGTGGGTGA GCCTGTGGCA 
CTGTCAGAAG AAGAGCGGCT GAAGCTGGCC CAGCAGCAGG CAGCTTTACT GATGCAGCAA 
GAGGAACGCG CCAAACAGGC AGCCGTATTA ATGGAGCAGG AACGGCAGCA GGAAATTGCC 
AAGATGGGCA CTGCAGTCCC TAGGCCTCCT CAGGATATGG GGCAGCTGGG GGTCCGTACT 
CCTCTGGGGC CTCGAGCTGC TCCAGTAGGC CCTGTGGTTC CCACACCTAC TGTTTTGCCC 
ATGGGGGCAC CTGTTCCTCG CCCTCGGGGT CCCCCGCCAC CCCCTGGAGA TGAGAACAGA 
GAGATGGATG ATCCTTCTGT GGGCCCCAAG ATCCCCCAGG CTTTGGAAAA GATTCTGCAG 
CTGAAGGAGA GCCGTCAGGA AGAGATGAAC TCCCAGCAGG AGGAAGAAGA AATGGAGACA 
GACACTCGCT CATCTCTGGG ACAGTCAGCA TCAGAGACTG AGGAGGACAC TGTGTCTACA 
TCCAAAAAAG AGAAAAACCG GAAGCGTCGG AACCGAAAGA AGAAGAAAAA GCCACAGCGG 
GTGCGGGCAG CCTCGTCAGA GAGCTCCGGG GACCGAGAGA AAGACTCAGC TCGGTCCCGA 
GGCTCTGACT CTCCTGCTGC TGATGTGGAG ATTGAGTATG TGACTGAAGA GCCTGAAATC 
TACGAGCCCA ACTTCATCTT CTTCAAGAGG ATTTTTGAGG CTTTCAAGCT AACTGATGAT 
GTGAAAAAGG AAAAGGAAAA GGAGCCAGAG AAGCTTGACA AGATGGAGAA CTCTGCAGTC 
CCCAAGAAGA AGGGCTTTGA GGAGGAGCAC AAAGACAGTG ACGATGACAG CAGTGACGAC 
GAACAGGAGA AGAAGCCGGA AGCCCCTAAG CTGTCCAAGA AGAAGCTGCG CCGGATGAAC 
CGCTTCACCG TGGCAGAGCT GAAGCAGCTG GTGGCCCGAC CTGATGTTGT TGAGATGCAT 
GACGTGACAG CACAGGACCC TAAGCTTTTG GTTCACCTCA AGGCCACCCG GAACTCTGTC 
CCTGTGCCAC GCCACTGGTG TTTTAAGCGC AAGTACCTTC AAGGCAAACG AGGCATTGAG 
AAACCCCCCT TTGAGCTGCC AGACTTCATC AAACGCACAG GCATTCAGGA GATGCGAGAG 
GCCCTGCAGG AGAAGGAAGA ACAGAAGACC ATGAAGTCAA AAATGAGAGA GAAGGTTCGG 
CCGAAGATGG GGAAGATTGA CATTGACTAC CAGAAGTTAC ACGATGCCTT CTTCAAGTGG 
CAGACCAAGC CGAAGCTCAC CATCCATGGG GACCTTTATT ACGAGGGGAA GGAGTTTGAG 
ACACGGCTGA AGGAGAAGAA GCCTGGAGAT TTGTCTGATG AGCTAAGGAT TTCTTTAGGG 
ATGCCAGTAG GACCTAATGC CCACAAAGTT CCTCCTCCGT GGCTGATTGC CATGCAGCGA 
TACGGGCCAC CGCCATCCTA CCCAAACCTG AAAATCCCTG GACTGAACTC ACCTATCCCT 
GAGAGCTGTC CTCTTGGATA CCATGCTGGT GGCTGGGGCA AACCCCCAGT AGATGAGACT 
GGGAAGCCAC TTTATGGGGA CGTGTTTGGA ACCAATGCTG CTGAGTTTCA GACCAAAACT 
GAAGAAGAAG AAATTGACCG GACTCCATGG GGGGAGCTGG AGCCATCGGA TGAAGAATCC 
TCAGAAGAGG AGGAAGAGGA GGAAAGCGAT GAAGACAAGC CAGATGAGAC TGGCTTTATC 
ACACCTGCGG ACAGTGGCCT CATCACTCCT GGAGGGTTCT CCTCCGTGCC AGCTGGAATG 
GAGACCCCTG AACTCATTGA GCTGAGAAAG AAGAAGATTG AAGAGGCGAT GGATGGGAGC 
GAGACACCTC AGTTGTTCAC TGTATTGCCA GAGAAGAGGA CAGCCACTGT TGGTGGGGCC 
ATGATGGGAT CTACCCACAT TTATGACATG TCCACGGTTA TGAGCCGGAA GGGCCCGGCC 
CCTGAGTTGC AAGGTGTGGA AGTAGCACTG GCTCCCGAGG AGTTGGAGTT GGACCCCATG 
GCCATGACCC AGAAGTATGA GGAGCATGTC CGGGAGCAGC AGGCCCAAGT CGAGAAAGAA 
GACTTCAGTG ACATGGTGGC GGAGCATGCT GCCAAACAGA AGCAAAAGAA ACGGAAAGCT 
CAGCCCCAGG ACAGTCGTGG GGGCAGCAAG AAATACAAGG AGTTCAAATT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of the human activated spliceosome in three conformational states.
Cell research28(3)307-322(2018 Mar)
Zhang X,Yan C,Zhan X,Li L,Lei J,Shi Y

Molecular Architecture of SF3b and Structural Consequences of Its Cancer-Related Mutations.
Molecular cell64(2)307-319(2016 10)
Cretu C,Schmitzová J,Ponce-Salvatierra A,Dybkov O,De Laurentiis EI,Sharma K,Will CL,Urlaub H,Lührmann R,Pena V

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanhäusser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors.
Nucleic acids research35(12)3928-44(2007)
Chen YI,Moore RE,Ge HY,Young MK,Lee TD,Stevens SW

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Sf3b2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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