Tpsb2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tpsb2
Entrez Gene ID	29268
Full Name	tryptase beta 2
Synonyms	Mcpt6
General protein information	Preferred Names tryptase beta 2 Names tryptase beta-2 mast cell protease 6 rMCP-6 tryptase-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12
Summary	serine protease; plays a role during the inflammatory process [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019180.2	NP_062053.2	tryptase beta-2 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC786948	XP_003587843.1
Macaca mulatta (Rhesus monkey)	TPSB2	XP_001088175.1
Mus musculus (house mouse)	Tpsb2	NP_034911.3
Canis lupus familiaris (dog)	LOC100049001	NP_001091024.1
Homo sapiens (human)	TPSB2	NP_077078.5
Homo sapiens (human)	TPSAB1	NP_003285.2
Bos taurus (cattle)	TPSB1	XP_005224652.1
Rattus norvegicus (Norway rat)	Tpsb2	NP_062053.2
Pan troglodytes (chimpanzee)	TPSB2	NP_001129130.1

Gene Ontology
Bibliography

Related articles in PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Mast cell maturation is driven via a group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis.
Taketomi Y, Ueno N, Kojima T, Sato H, Murase R, Yamamoto K, Tanaka S, Sakanaka M, Nakamura M, Nishito Y, Kawana M, Kambe N, Ikeda K, Taguchi R, Nakamizo S, Kabashima K, Gelb MH, Arita M, Yokomizo T, Nakamura M, Watanabe K, Hirai H, Nakamura M, Okayama Y, Ra C, Aritake K, Urade Y, Morimoto K, Sugimoto Y, Shimizu T, Narumiya S, Hara S, Murakami M
Nature immunology14(6)554-63(2013 Jun)

Structural requirements and mechanism for heparin-induced activation of a recombinant mouse mast cell tryptase, mouse mast cell protease-6: formation of active tryptase monomers in the presence of low molecular weight heparin.
Hallgren J, Spillmann D, Pejler G
The Journal of biological chemistry276(46)42774-81(2001 Nov)

Secretory granule proteases in rat mast cells. Cloning of 10 different serine proteases and a carboxypeptidase A from various rat mast cell populations.
Lützelschwab C, Pejler G, Aveskogh M, Hellman L
The Journal of experimental medicine185(1)13-29(1997 Jan)

cDNA sequencing and expression of rat mast cell tryptase.
Ide H, Itoh H, Tomita M, Murakumo Y, Kobayashi T, Maruyama H, Osada Y, Nawa Y
Journal of biochemistry118(1)210-5(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tpsb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tpsb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03953	NM_019180.2 Latest version!	Rattus norvegicus tryptase beta 2 (Tpsb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tpsb2 ( NM_019180.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062053.2 Rattus norvegicus tryptase beta 2 (Tpsb2), mRNA.

Price & Availability↑

CloneID	ORa03953
Clone ID Related Accession (Same CDS sequence)	NM_019180.2
Accession Version	NM_019180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Rattus norvegicus(Norway rat)
Product	tryptase beta-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U67909.1. On Jun 3, 2010 this sequence version replaced NM_019180.1. ##Evidence-Data-START## Transcript exon combination :: U67909.1, D38455.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMN00632251 [ECO:0000348] ##Evidence-Data-END##

ATGCTGAAGC TGCTGCTGCT GCTGGCACTG TCCCCCCTGG CTAGTCTGGT GCACGCGGCC 
CCTTGCCCAG TCAAGCAGCG AGTGGGCATT GTGGGAGGAC GAGAGGCTTC TGAAAGTAAG 
TGGCCCTGGC AGGTGAGCCT GAGATTTAAA TTCAGCTTCT GGATGCATTT CTGTGGCGGC 
TCCCTCATTC ACCCACAGTG GGTGCTCACT GCGGCACACT GTGTGGGACT GCACATCAAA 
AGCCCAGAGC TCTTCCGTGT ACAGCTTCGT GAGCAGTATC TATACTATGC GGACCAGCTA 
CTGACTGTGA ACCGGACCGT TGTGCACCCC CACTACTACA CAGTCGAGGA TGGGGCAGAC 
ATTGCCCTGC TGGAGCTTGA GAACCCTGTG AATGTCTCCA CCCATATCCA CCCCACATCC 
CTGCCCCCTG CCTCGGAGAC CTTCCCCTCG GGGACTTCTT GCTGGGTAAC AGGCTGGGGC 
GACATTGATA GTGACGAGCC TCTCCTGCCA CCTTATCCTC TGAAGCAAGT GAAGGTCCCC 
ATTGTGGAAA ACAGCCTGTG TGATCGGAAG TACCACACTG GCCTCTACAC AGGAGATGAT 
GTTCCCATTG TCCAGGATGG CATGCTGTGT GCTGGAAATA CCAGGAGCGA CTCCTGCCAG 
GGAGACTCAG GGGGCCCACT GGTCTGCAAA GTGAAGGGTA CCTGGCTGCA AGCAGGAGTG 
GTCAGCTGGG GCGAGGGCTG CGCAGAGGCC AATCGTCCTG GCATTTACAC CCGGGTGACG 
TACTACCTGG ACTGGATTCA CCGCTATGTC CCTCAGCGTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062053.2
CDS	16..840
Translation	MLKLLLLLALSPLASLVHAAPCPVKQRVGIVGGREASESKWPWQVSLRFKFSFWMHFCGGSLIHPQWVLTAAHCVGLHIKSPELFRVQLREQYLYYADQLLTVNRTVVHPHYYTVEDGADIALLELENPVNVSTHIHPTSLPPASETFPSGTSCWVTGWGDIDSDEPLLPPYPLKQVKVPIVENSLCDRKYHTGLYTGDDVPIVQDGMLCAGNTRSDSCQGDSGGPLVCKVKGTWLQAGVVSWGEGCAEANRPGIYTRVTYYLDWIHRYVPQRS

Target ORF information:

RefSeq Version	NM_019180.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tryptase beta 2 (Tpsb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019180.2

ORF Insert Sequence:

ATGCTGAAGC TGCTGCTGCT GCTGGCACTG TCCCCCCTGG CTAGTCTGGT GCACGCGGCC 
CCTTGCCCAG TCAAGCAGCG AGTGGGCATT GTGGGAGGAC GAGAGGCTTC TGAAAGTAAG 
TGGCCCTGGC AGGTGAGCCT GAGATTTAAA TTCAGCTTCT GGATGCATTT CTGTGGCGGC 
TCCCTCATTC ACCCACAGTG GGTGCTCACT GCGGCACACT GTGTGGGACT GCACATCAAA 
AGCCCAGAGC TCTTCCGTGT ACAGCTTCGT GAGCAGTATC TATACTATGC GGACCAGCTA 
CTGACTGTGA ACCGGACCGT TGTGCACCCC CACTACTACA CAGTCGAGGA TGGGGCAGAC 
ATTGCCCTGC TGGAGCTTGA GAACCCTGTG AATGTCTCCA CCCATATCCA CCCCACATCC 
CTGCCCCCTG CCTCGGAGAC CTTCCCCTCG GGGACTTCTT GCTGGGTAAC AGGCTGGGGC 
GACATTGATA GTGACGAGCC TCTCCTGCCA CCTTATCCTC TGAAGCAAGT GAAGGTCCCC 
ATTGTGGAAA ACAGCCTGTG TGATCGGAAG TACCACACTG GCCTCTACAC AGGAGATGAT 
GTTCCCATTG TCCAGGATGG CATGCTGTGT GCTGGAAATA CCAGGAGCGA CTCCTGCCAG 
GGAGACTCAG GGGGCCCACT GGTCTGCAAA GTGAAGGGTA CCTGGCTGCA AGCAGGAGTG 
GTCAGCTGGG GCGAGGGCTG CGCAGAGGCC AATCGTCCTG GCATTTACAC CCGGGTGACG 
TACTACCTGG ACTGGATTCA CCGCTATGTC CCTCAGCGTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Mast cell maturation is driven via a group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis.
Nature immunology14(6)554-63(2013 Jun)
Taketomi Y,Ueno N,Kojima T,Sato H,Murase R,Yamamoto K,Tanaka S,Sakanaka M,Nakamura M,Nishito Y,Kawana M,Kambe N,Ikeda K,Taguchi R,Nakamizo S,Kabashima K,Gelb MH,Arita M,Yokomizo T,Nakamura M,Watanabe K,Hirai H,Nakamura M,Okayama Y,Ra C,Aritake K,Urade Y,Morimoto K,Sugimoto Y,Shimizu T,Narumiya S,Hara S,Murakami M

Structural requirements and mechanism for heparin-induced activation of a recombinant mouse mast cell tryptase, mouse mast cell protease-6: formation of active tryptase monomers in the presence of low molecular weight heparin.
The Journal of biological chemistry276(46)42774-81(2001 Nov)
Hallgren J,Spillmann D,Pejler G

Secretory granule proteases in rat mast cells. Cloning of 10 different serine proteases and a carboxypeptidase A from various rat mast cell populations.
The Journal of experimental medicine185(1)13-29(1997 Jan)
Lützelschwab C,Pejler G,Aveskogh M,Hellman L

cDNA sequencing and expression of rat mast cell tryptase.
Journal of biochemistry118(1)210-5(1995 Jul)
Ide H,Itoh H,Tomita M,Murakumo Y,Kobayashi T,Maruyama H,Osada Y,Nawa Y

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Tpsb2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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