Megf10 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Megf10
Entrez Gene ID	291445
Full Name	multiple EGF-like domains 10
General protein information	Preferred Names multiple EGF-like domains 10 Names multiple epidermal growth factor-like domains protein 10
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18q12.1
Summary	putative central nervous system protein; transcript recognized by a monoclonal autoantibody which stimulates remyelination of Theiler's virus-demyelinated neurons [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006254747.2	XP_006254809.1	multiple epidermal growth factor-like domains protein 10 isoform X1
NM_001100657.1	NP_001094127.1	multiple epidermal growth factor-like domains protein 10 precursor
XM_017600884.1	XP_017456373.1	multiple epidermal growth factor-like domains protein 10 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Megf10	NP_001001979.1
Danio rerio (zebrafish)	megf10	XP_001922524.2
Xenopus tropicalis (tropical clawed frog)	megf10	NP_001072726.1
Pan troglodytes (chimpanzee)	MEGF10	XP_517907.2
Bos taurus (cattle)	MEGF10	NP_001039916.2
Macaca mulatta (Rhesus monkey)	MEGF10	XP_001097941.1
Canis lupus familiaris (dog)	MEGF10	XP_005626533.1
Homo sapiens (human)	MEGF10	NP_115822.1
Rattus norvegicus (Norway rat)	Megf10	NP_001094127.1
Gallus gallus (chicken)	MEGF10	XP_424719.3

Gene Ontology
Bibliography

Related articles in PubMed

Consequences of MEGF10 deficiency on myoblast function and Notch1 interactions.
Saha M, Mitsuhashi S, Jones MD, Manko K, Reddy HM, Bruels CC, Cho KA, Pacak CA, Draper I, Kang PB
Human molecular genetics26(15)2984-3000(2017 08)

Megf10 Is a Receptor for C1Q That Mediates Clearance of Apoptotic Cells by Astrocytes.
Iram T, Ramirez-Ortiz Z, Byrne MH, Coleman UA, Kingery ND, Means TK, Frenkel D, El Khoury J
The Journal of neuroscience : the official journal of the Society for Neuroscience36(19)5185-92(2016 05)

MEGF10 and MEGF11 mediate homotypic interactions required for mosaic spacing of retinal neurons.
Kay JN, Chu MW, Sanes JR
Nature483(7390)465-9(2012 Mar)

Mutations in MEGF10, a regulator of satellite cell myogenesis, cause early onset myopathy, areflexia, respiratory distress and dysphagia (EMARDD).
Logan CV, Lucke B, Pottinger C, Abdelhamed ZA, Parry DA, Szymanska K, Diggle CP, van Riesen A, Morgan JE, Markham G, Ellis I, Manzur AY, Markham AF, Shires M, Helliwell T, Scoto M, Hübner C, Bonthron DT, Taylor GR, Sheridan E, Muntoni F, Carr IM, Schuelke M, Johnson CA
Nature genetics43(12)1189-92(2011 Nov)

Glial precursors clear sensory neuron corpses during development via Jedi-1, an engulfment receptor.
Wu HH, Bellmunt E, Scheib JL, Venegas V, Burkert C, Reichardt LF, Zhou Z, Fariñas I, Carter BD
Nature neuroscience12(12)1534-41(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Megf10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Megf10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04464	NM_001100657.1 Latest version!	Rattus norvegicus multiple EGF-like domains 10 (Megf10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa04464	XM_006254747.2 Latest version!	Rattus norvegicus multiple EGF-like domains 10 (Megf10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa04464	XM_017600884.1 Latest version!	Rattus norvegicus multiple EGF-like domains 10 (Megf10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Megf10 ( NM_001100657.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094127.1 Rattus norvegicus multiple EGF-like domains 10 (Megf10), mRNA.

Price & Availability↑

CloneID	ORa04464
Clone ID Related Accession (Same CDS sequence)	XM_006254747.2 , NM_001100657.1 , XM_017600884.1
Accession Version	NM_001100657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Rattus norvegicus(Norway rat)
Product	multiple epidermal growth factor-like domains protein 10 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07072598.1. On or before Dec 19, 2009 this sequence version replaced XM_001059780.1, XM_238019.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTATTT CTTCAAGCTC CTGCCTGGGC CTCATCTGCT CACTGCTGTG CCACTGGCTG 
AGGGCAGCAT CCTCACTGAA CCTGGAAGAC CCCAATGTAT GCAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAGGAGTC TTACCCACAT CCCTTCGATC AGATTTACTA CACAAGCTGC 
ACTGACATAC TGAACTGGTT TAAATGCACT CGGCACAGAG TGAGCTACCG GACAGCCTAC 
CGCCATGGGG AGAAAACCAT GTACAGACGC AAATCCCAGT GTTGCCCCGG ATTTTATGAA 
AGTCGAGACG TGTGTGTCCC TCACTGCGCT GATAAATGCG TCCACGGTCG CTGCATCGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGGACCA ACTGTAGCAG TGCTTGTGAC 
GGTGATCACT GGGGACCTCA CTGCAGCAGC CGGTGTCAGT GCAAAAACAG AGCTTTGTGT 
AACCCCATCA CCGGAGCTTG TCACTGCGCC GCGGGCTACC GGGGATGGCG CTGTGAGGAC 
CGTTGTGAAC AGGGCACCTA CGGTAACGAC TGTCATCAAA GGTGCCAGTG TCAGAATGGA 
GCGACCTGTG ACCACATCAC TGGGGAATGT CGTTGTTCAC CTGGATACAC TGGAGCCTTC 
TGTGAGGATC TGTGTCCTCC TGGCAAACAT GGCCCACAGT GTGAGCAGAG ATGTCCCTGC 
CAAAATGGAG GCGTGTGCCA TCATGTCACT GGAGAGTGCT CCTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC CTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGTCAGTGCC ACAATGGAGG AGCGTGTGAT GCTGCCACAG GCCAATGTCA CTGCAGCCCA 
GGATACACAG GGGAACGGTG TCAGGATGAA TGTCCTGTTG GAACCTATGG AGTTCGCTGT 
GCTGAGACCT GCAGGTGTGT TAATGGAGGG AAGTGTTACC ATGTGAGCGG CACATGCCTA 
TGCGAAGCAG GTTTTTCAGG TGAATTTTGC GAGGCTCGTC TGTGCCCTGA GGGGCTTTAC 
GGCATCAAAT GTGACAAGCG GTGCCCCTGC CACCTGGACA ATACTCATAG CTGTCATCCC 
ATGTCTGGAG AGTGTGGCTG CAAGCCGGGT TGGTCTGGAC TGTACTGTAA TGAAACATGC 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCAAAA CGGGGCTGAC 
TGTGACAGTG TGAGTGGAAG GTGTGCCTGT GCTCCAGGAT TCAAAGGAAC TGACTGCTCT 
ACTCCGTGTC CTCTGGGACG CTATGGGATA AATTGTTCTT CTCGCTGTGG CTGTAAAAAC 
GATGCTGTCT GTTCTCCTGT GGATGGATCG TGCATCTGTA AGGCAGGCTG GCACGGGGTG 
GACTGTTCCA TCAGCTGCCC CAGTGGCACA TGGGGCTTTG GCTGTAATCT AACCTGTCAG 
TGCCTCAATG GCGGTGCCTG CAACACACTG GACGGCACCT GCACCTGTGC GCCGGGATGG 
CGAGGGGAGA AATGTGAATT TCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCTGAG 
CGCTGTGACT GCAGCCATGC AGACGGATGT CACCCCACTA CCGGCCATTG CCGATGCCTC 
CCTGGATGGT CAGGTGTGCA CTGTGACAGT GTGTGTGCTG AGGGACGCTG GGGTCCTAAC 
TGCTCGCTGC CCTGCTACTG TAAAAATGGG GCTTCGTGTT CTCCCGATGA TGGCATCTGT 
GAATGCGCAC CCGGATTCCG AGGCACCACT TGCCAGAGAA TCTGCTCCCC GGGCTTTTAT 
GGACACCGCT GTAGCCAGAC ATGCCCACAG TGTGTGCACA GCAGTGGACC ATGCCACCAC 
ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACTGGCG CCCTGTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA GAACTGTGCA GGTGTTTGTA CCTGCACCAA CAACGGCACC 
TGTAACCCCA TCGACCGGTC CTGTCAGTGC TACCCAGGCT GGATTGGCAG TGACTGCTCC 
CAGCCCTGTC CACCAGCCCA CTGGGGTCCG AACTGCATCC ATACATGCAA CTGCCACAAT 
GGAGCCTTTT GCAGCGCCTA TGATGGGGAA TGCAAATGCA CTCCTGGCTG GACGGGGCTC 
TACTGCACTC AGAGATGCCC TCTGGGCTTC TATGGCAAGG ACTGTGCACT GGTATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATC TCGGGGCAGT GTACCTGCCG CACGGGATTC 
ATGGGACGGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ACGGCTATGG CTGTCGCCAG 
ATCTGCGACT GTCTGAACAA CTCCACCTGT GACCACATCA CCGGCACGTG TTACTGTAGC 
CCAGGGTGGA AAGGAGCACG ATGTGACCAA GCTGGGGTCA TCATCGTGGG CAATCTGAAC 
AGCTTAAGCA GGACCAGCAC CGCCCTTCCT GCTGACTCCT ATCAGATTGG GGCCATCGCG 
GGCATCGTGG TCCTTCTGCT TGTTGTGCTC TTCCTGCTGG CGCTGTTCGT TATCTACAGA 
CACAAGCAGA AGAGGAAGGA ATCGAGCATG CCGGCCGTGA CCTACACCCC TGCCATGAGG 
GTTATCAATG CAGACTATAC CATTGCAGAA ACCCTGCCTC ACAGCAATGG CGGAAATGCC 
AACAGCCACT ACTTCACCAA TCCCAGTTAT CACACACTTA GCCAGTGTGC CGCGTCCCCT 
CATGTGAACA ACAGGGACAG GATGACCATT GCAAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAATCC AGGGAAGAGA GGCGCTTTGG TGGACTGCAC TGGGACGTTG 
CCAGCTGACT GGAAGCAAGG AGGCTACCTC AATGAACTTG GTGCTTTTGG GCTGGACAGA 
AGCTACATGG GAAAGTCCTT AAAAGATCTG GGGAAGAATT CTGAGTACAA TTCAAGTACT 
TGTTCCCTAA GCAGCTCGGA GAACCCATAT GCCACCATTA AAGACCCTCC TGCGCTCTTG 
CCTAAAAGCT CCGAGTGTGG CTACGTGGAG ATGAAGTCGC CAGCGCGAAG AGACTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT CGAACCTACA 
GTCAGTGTGG TGCAAGGAGT ATTCAGCAAC AATGGTCATG TCACCCAAGA CCCATATGAC 
CTTCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTAAG GGACAGTTCA 
TCCTCCCCAA AGAGAGAGGA TGGTGGTGGC AGCAGCAGCA GCACCACCAC CAGCAACAGC 
AGCAACAGCA GCGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094127.1
CDS	18..3455
Translation	MAISSSSCLGLICSLLCHWLRAASSLNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCTRHRVSYRTAYRHGEKTMYRRKSQCCPGFYESRDVCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACDGDHWGPHCSSRCQCKNRALCNPITGACHCAAGYRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHITGECRCSPGYTGAFCEDLCPPGKHGPQCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQECQCHNGGACDAATGQCHCSPGYTGERCQDECPVGTYGVRCAETCRCVNGGKCYHVSGTCLCEAGFSGEFCEARLCPEGLYGIKCDKRCPCHLDNTHSCHPMSGECGCKPGWSGLYCNETCSPGFYGEACQQICSCQNGADCDSVSGRCACAPGFKGTDCSTPCPLGRYGINCSSRCGCKNDAVCSPVDGSCICKAGWHGVDCSISCPSGTWGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCEFPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCLPGWSGVHCDSVCAEGRWGPNCSLPCYCKNGASCSPDDGICECAPGFRGTTCQRICSPGFYGHRCSQTCPQCVHSSGPCHHITGLCDCLPGFTGALCNEVCPSGRFGKNCAGVCTCTNNGTCNPIDRSCQCYPGWIGSDCSQPCPPAHWGPNCIHTCNCHNGAFCSAYDGECKCTPGWTGLYCTQRCPLGFYGKDCALVCQCQNGADCDHISGQCTCRTGFMGRHCEQKCPAGTYGYGCRQICDCLNNSTCDHITGTCYCSPGWKGARCDQAGVIIVGNLNSLSRTSTALPADSYQIGAIAGIVVLLLVVLFLLALFVIYRHKQKRKESSMPAVTYTPAMRVINADYTIAETLPHSNGGNANSHYFTNPSYHTLSQCAASPHVNNRDRMTIAKSKNNQLFVNLKNVNPGKRGALVDCTGTLPADWKQGGYLNELGAFGLDRSYMGKSLKDLGKNSEYNSSTCSLSSSENPYATIKDPPALLPKSSECGYVEMKSPARRDSPYAEINNSTSANRNVYEVEPTVSVVQGVFSNNGHVTQDPYDLPKNSHIPCHYDLLPVRDSSSSPKREDGGGSSSSTTTSNSSNSSE

Target ORF information:

RefSeq Version	NM_001100657.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus multiple EGF-like domains 10 (Megf10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100657.1

ORF Insert Sequence:

ATGGCTATTT CTTCAAGCTC CTGCCTGGGC CTCATCTGCT CACTGCTGTG CCACTGGCTG 
AGGGCAGCAT CCTCACTGAA CCTGGAAGAC CCCAATGTAT GCAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAGGAGTC TTACCCACAT CCCTTCGATC AGATTTACTA CACAAGCTGC 
ACTGACATAC TGAACTGGTT TAAATGCACT CGGCACAGAG TGAGCTACCG GACAGCCTAC 
CGCCATGGGG AGAAAACCAT GTACAGACGC AAATCCCAGT GTTGCCCCGG ATTTTATGAA 
AGTCGAGACG TGTGTGTCCC TCACTGCGCT GATAAATGCG TCCACGGTCG CTGCATCGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGGACCA ACTGTAGCAG TGCTTGTGAC 
GGTGATCACT GGGGACCTCA CTGCAGCAGC CGGTGTCAGT GCAAAAACAG AGCTTTGTGT 
AACCCCATCA CCGGAGCTTG TCACTGCGCC GCGGGCTACC GGGGATGGCG CTGTGAGGAC 
CGTTGTGAAC AGGGCACCTA CGGTAACGAC TGTCATCAAA GGTGCCAGTG TCAGAATGGA 
GCGACCTGTG ACCACATCAC TGGGGAATGT CGTTGTTCAC CTGGATACAC TGGAGCCTTC 
TGTGAGGATC TGTGTCCTCC TGGCAAACAT GGCCCACAGT GTGAGCAGAG ATGTCCCTGC 
CAAAATGGAG GCGTGTGCCA TCATGTCACT GGAGAGTGCT CCTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC CTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGTCAGTGCC ACAATGGAGG AGCGTGTGAT GCTGCCACAG GCCAATGTCA CTGCAGCCCA 
GGATACACAG GGGAACGGTG TCAGGATGAA TGTCCTGTTG GAACCTATGG AGTTCGCTGT 
GCTGAGACCT GCAGGTGTGT TAATGGAGGG AAGTGTTACC ATGTGAGCGG CACATGCCTA 
TGCGAAGCAG GTTTTTCAGG TGAATTTTGC GAGGCTCGTC TGTGCCCTGA GGGGCTTTAC 
GGCATCAAAT GTGACAAGCG GTGCCCCTGC CACCTGGACA ATACTCATAG CTGTCATCCC 
ATGTCTGGAG AGTGTGGCTG CAAGCCGGGT TGGTCTGGAC TGTACTGTAA TGAAACATGC 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCAAAA CGGGGCTGAC 
TGTGACAGTG TGAGTGGAAG GTGTGCCTGT GCTCCAGGAT TCAAAGGAAC TGACTGCTCT 
ACTCCGTGTC CTCTGGGACG CTATGGGATA AATTGTTCTT CTCGCTGTGG CTGTAAAAAC 
GATGCTGTCT GTTCTCCTGT GGATGGATCG TGCATCTGTA AGGCAGGCTG GCACGGGGTG 
GACTGTTCCA TCAGCTGCCC CAGTGGCACA TGGGGCTTTG GCTGTAATCT AACCTGTCAG 
TGCCTCAATG GCGGTGCCTG CAACACACTG GACGGCACCT GCACCTGTGC GCCGGGATGG 
CGAGGGGAGA AATGTGAATT TCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCTGAG 
CGCTGTGACT GCAGCCATGC AGACGGATGT CACCCCACTA CCGGCCATTG CCGATGCCTC 
CCTGGATGGT CAGGTGTGCA CTGTGACAGT GTGTGTGCTG AGGGACGCTG GGGTCCTAAC 
TGCTCGCTGC CCTGCTACTG TAAAAATGGG GCTTCGTGTT CTCCCGATGA TGGCATCTGT 
GAATGCGCAC CCGGATTCCG AGGCACCACT TGCCAGAGAA TCTGCTCCCC GGGCTTTTAT 
GGACACCGCT GTAGCCAGAC ATGCCCACAG TGTGTGCACA GCAGTGGACC ATGCCACCAC 
ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACTGGCG CCCTGTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA GAACTGTGCA GGTGTTTGTA CCTGCACCAA CAACGGCACC 
TGTAACCCCA TCGACCGGTC CTGTCAGTGC TACCCAGGCT GGATTGGCAG TGACTGCTCC 
CAGCCCTGTC CACCAGCCCA CTGGGGTCCG AACTGCATCC ATACATGCAA CTGCCACAAT 
GGAGCCTTTT GCAGCGCCTA TGATGGGGAA TGCAAATGCA CTCCTGGCTG GACGGGGCTC 
TACTGCACTC AGAGATGCCC TCTGGGCTTC TATGGCAAGG ACTGTGCACT GGTATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATC TCGGGGCAGT GTACCTGCCG CACGGGATTC 
ATGGGACGGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ACGGCTATGG CTGTCGCCAG 
ATCTGCGACT GTCTGAACAA CTCCACCTGT GACCACATCA CCGGCACGTG TTACTGTAGC 
CCAGGGTGGA AAGGAGCACG ATGTGACCAA GCTGGGGTCA TCATCGTGGG CAATCTGAAC 
AGCTTAAGCA GGACCAGCAC CGCCCTTCCT GCTGACTCCT ATCAGATTGG GGCCATCGCG 
GGCATCGTGG TCCTTCTGCT TGTTGTGCTC TTCCTGCTGG CGCTGTTCGT TATCTACAGA 
CACAAGCAGA AGAGGAAGGA ATCGAGCATG CCGGCCGTGA CCTACACCCC TGCCATGAGG 
GTTATCAATG CAGACTATAC CATTGCAGAA ACCCTGCCTC ACAGCAATGG CGGAAATGCC 
AACAGCCACT ACTTCACCAA TCCCAGTTAT CACACACTTA GCCAGTGTGC CGCGTCCCCT 
CATGTGAACA ACAGGGACAG GATGACCATT GCAAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAATCC AGGGAAGAGA GGCGCTTTGG TGGACTGCAC TGGGACGTTG 
CCAGCTGACT GGAAGCAAGG AGGCTACCTC AATGAACTTG GTGCTTTTGG GCTGGACAGA 
AGCTACATGG GAAAGTCCTT AAAAGATCTG GGGAAGAATT CTGAGTACAA TTCAAGTACT 
TGTTCCCTAA GCAGCTCGGA GAACCCATAT GCCACCATTA AAGACCCTCC TGCGCTCTTG 
CCTAAAAGCT CCGAGTGTGG CTACGTGGAG ATGAAGTCGC CAGCGCGAAG AGACTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT CGAACCTACA 
GTCAGTGTGG TGCAAGGAGT ATTCAGCAAC AATGGTCATG TCACCCAAGA CCCATATGAC 
CTTCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTAAG GGACAGTTCA 
TCCTCCCCAA AGAGAGAGGA TGGTGGTGGC AGCAGCAGCA GCACCACCAC CAGCAACAGC 
AGCAACAGCA GCGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Megf10 ( XM_006254747.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254809.1 Rattus norvegicus multiple EGF-like domains 10 (Megf10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa04464
Clone ID Related Accession (Same CDS sequence)	XM_006254747.2 , NM_001100657.1 , XM_017600884.1
Accession Version	XM_006254747.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	multiple epidermal growth factor-like domains protein 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTATTT CTTCAAGCTC CTGCCTGGGC CTCATCTGCT CACTGCTGTG CCACTGGCTG 
AGGGCAGCAT CCTCACTGAA CCTGGAAGAC CCCAATGTAT GCAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAGGAGTC TTACCCACAT CCCTTCGATC AGATTTACTA CACAAGCTGC 
ACTGACATAC TGAACTGGTT TAAATGCACT CGGCACAGAG TGAGCTACCG GACAGCCTAC 
CGCCATGGGG AGAAAACCAT GTACAGACGC AAATCCCAGT GTTGCCCCGG ATTTTATGAA 
AGTCGAGACG TGTGTGTCCC TCACTGCGCT GATAAATGCG TCCACGGTCG CTGCATCGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGGACCA ACTGTAGCAG TGCTTGTGAC 
GGTGATCACT GGGGACCTCA CTGCAGCAGC CGGTGTCAGT GCAAAAACAG AGCTTTGTGT 
AACCCCATCA CCGGAGCTTG TCACTGCGCC GCGGGCTACC GGGGATGGCG CTGTGAGGAC 
CGTTGTGAAC AGGGCACCTA CGGTAACGAC TGTCATCAAA GGTGCCAGTG TCAGAATGGA 
GCGACCTGTG ACCACATCAC TGGGGAATGT CGTTGTTCAC CTGGATACAC TGGAGCCTTC 
TGTGAGGATC TGTGTCCTCC TGGCAAACAT GGCCCACAGT GTGAGCAGAG ATGTCCCTGC 
CAAAATGGAG GCGTGTGCCA TCATGTCACT GGAGAGTGCT CCTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC CTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGTCAGTGCC ACAATGGAGG AGCGTGTGAT GCTGCCACAG GCCAATGTCA CTGCAGCCCA 
GGATACACAG GGGAACGGTG TCAGGATGAA TGTCCTGTTG GAACCTATGG AGTTCGCTGT 
GCTGAGACCT GCAGGTGTGT TAATGGAGGG AAGTGTTACC ATGTGAGCGG CACATGCCTA 
TGCGAAGCAG GTTTTTCAGG TGAATTTTGC GAGGCTCGTC TGTGCCCTGA GGGGCTTTAC 
GGCATCAAAT GTGACAAGCG GTGCCCCTGC CACCTGGACA ATACTCATAG CTGTCATCCC 
ATGTCTGGAG AGTGTGGCTG CAAGCCGGGT TGGTCTGGAC TGTACTGTAA TGAAACATGC 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCAAAA CGGGGCTGAC 
TGTGACAGTG TGAGTGGAAG GTGTGCCTGT GCTCCAGGAT TCAAAGGAAC TGACTGCTCT 
ACTCCGTGTC CTCTGGGACG CTATGGGATA AATTGTTCTT CTCGCTGTGG CTGTAAAAAC 
GATGCTGTCT GTTCTCCTGT GGATGGATCG TGCATCTGTA AGGCAGGCTG GCACGGGGTG 
GACTGTTCCA TCAGCTGCCC CAGTGGCACA TGGGGCTTTG GCTGTAATCT AACCTGTCAG 
TGCCTCAATG GCGGTGCCTG CAACACACTG GACGGCACCT GCACCTGTGC GCCGGGATGG 
CGAGGGGAGA AATGTGAATT TCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCTGAG 
CGCTGTGACT GCAGCCATGC AGACGGATGT CACCCCACTA CCGGCCATTG CCGATGCCTC 
CCTGGATGGT CAGGTGTGCA CTGTGACAGT GTGTGTGCTG AGGGACGCTG GGGTCCTAAC 
TGCTCGCTGC CCTGCTACTG TAAAAATGGG GCTTCGTGTT CTCCCGATGA TGGCATCTGT 
GAATGCGCAC CCGGATTCCG AGGCACCACT TGCCAGAGAA TCTGCTCCCC GGGCTTTTAT 
GGACACCGCT GTAGCCAGAC ATGCCCACAG TGTGTGCACA GCAGTGGACC ATGCCACCAC 
ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACTGGCG CCCTGTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA GAACTGTGCA GGTGTTTGTA CCTGCACCAA CAACGGCACC 
TGTAACCCCA TCGACCGGTC CTGTCAGTGC TACCCAGGCT GGATTGGCAG TGACTGCTCC 
CAGCCCTGTC CACCAGCCCA CTGGGGTCCG AACTGCATCC ATACATGCAA CTGCCACAAT 
GGAGCCTTTT GCAGCGCCTA TGATGGGGAA TGCAAATGCA CTCCTGGCTG GACGGGGCTC 
TACTGCACTC AGAGATGCCC TCTGGGCTTC TATGGCAAGG ACTGTGCACT GGTATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATC TCGGGGCAGT GTACCTGCCG CACGGGATTC 
ATGGGACGGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ACGGCTATGG CTGTCGCCAG 
ATCTGCGACT GTCTGAACAA CTCCACCTGT GACCACATCA CCGGCACGTG TTACTGTAGC 
CCAGGGTGGA AAGGAGCACG ATGTGACCAA GCTGGGGTCA TCATCGTGGG CAATCTGAAC 
AGCTTAAGCA GGACCAGCAC CGCCCTTCCT GCTGACTCCT ATCAGATTGG GGCCATCGCG 
GGCATCGTGG TCCTTCTGCT TGTTGTGCTC TTCCTGCTGG CGCTGTTCGT TATCTACAGA 
CACAAGCAGA AGAGGAAGGA ATCGAGCATG CCGGCCGTGA CCTACACCCC TGCCATGAGG 
GTTATCAATG CAGACTATAC CATTGCAGAA ACCCTGCCTC ACAGCAATGG CGGAAATGCC 
AACAGCCACT ACTTCACCAA TCCCAGTTAT CACACACTTA GCCAGTGTGC CGCGTCCCCT 
CATGTGAACA ACAGGGACAG GATGACCATT GCAAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAATCC AGGGAAGAGA GGCGCTTTGG TGGACTGCAC TGGGACGTTG 
CCAGCTGACT GGAAGCAAGG AGGCTACCTC AATGAACTTG GTGCTTTTGG GCTGGACAGA 
AGCTACATGG GAAAGTCCTT AAAAGATCTG GGGAAGAATT CTGAGTACAA TTCAAGTACT 
TGTTCCCTAA GCAGCTCGGA GAACCCATAT GCCACCATTA AAGACCCTCC TGCGCTCTTG 
CCTAAAAGCT CCGAGTGTGG CTACGTGGAG ATGAAGTCGC CAGCGCGAAG AGACTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT CGAACCTACA 
GTCAGTGTGG TGCAAGGAGT ATTCAGCAAC AATGGTCATG TCACCCAAGA CCCATATGAC 
CTTCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTAAG GGACAGTTCA 
TCCTCCCCAA AGAGAGAGGA TGGTGGTGGC AGCAGCAGCA GCACCACCAC CAGCAACAGC 
AGCAACAGCA GCGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254809.1
CDS	758..4195
Translation	MAISSSSCLGLICSLLCHWLRAASSLNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCTRHRVSYRTAYRHGEKTMYRRKSQCCPGFYESRDVCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACDGDHWGPHCSSRCQCKNRALCNPITGACHCAAGYRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHITGECRCSPGYTGAFCEDLCPPGKHGPQCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQECQCHNGGACDAATGQCHCSPGYTGERCQDECPVGTYGVRCAETCRCVNGGKCYHVSGTCLCEAGFSGEFCEARLCPEGLYGIKCDKRCPCHLDNTHSCHPMSGECGCKPGWSGLYCNETCSPGFYGEACQQICSCQNGADCDSVSGRCACAPGFKGTDCSTPCPLGRYGINCSSRCGCKNDAVCSPVDGSCICKAGWHGVDCSISCPSGTWGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCEFPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCLPGWSGVHCDSVCAEGRWGPNCSLPCYCKNGASCSPDDGICECAPGFRGTTCQRICSPGFYGHRCSQTCPQCVHSSGPCHHITGLCDCLPGFTGALCNEVCPSGRFGKNCAGVCTCTNNGTCNPIDRSCQCYPGWIGSDCSQPCPPAHWGPNCIHTCNCHNGAFCSAYDGECKCTPGWTGLYCTQRCPLGFYGKDCALVCQCQNGADCDHISGQCTCRTGFMGRHCEQKCPAGTYGYGCRQICDCLNNSTCDHITGTCYCSPGWKGARCDQAGVIIVGNLNSLSRTSTALPADSYQIGAIAGIVVLLLVVLFLLALFVIYRHKQKRKESSMPAVTYTPAMRVINADYTIAETLPHSNGGNANSHYFTNPSYHTLSQCAASPHVNNRDRMTIAKSKNNQLFVNLKNVNPGKRGALVDCTGTLPADWKQGGYLNELGAFGLDRSYMGKSLKDLGKNSEYNSSTCSLSSSENPYATIKDPPALLPKSSECGYVEMKSPARRDSPYAEINNSTSANRNVYEVEPTVSVVQGVFSNNGHVTQDPYDLPKNSHIPCHYDLLPVRDSSSSPKREDGGGSSSSTTTSNSSNSSE

Target ORF information:

RefSeq Version	XM_006254747.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus multiple EGF-like domains 10 (Megf10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254747.2

ORF Insert Sequence:

ATGGCTATTT CTTCAAGCTC CTGCCTGGGC CTCATCTGCT CACTGCTGTG CCACTGGCTG 
AGGGCAGCAT CCTCACTGAA CCTGGAAGAC CCCAATGTAT GCAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAGGAGTC TTACCCACAT CCCTTCGATC AGATTTACTA CACAAGCTGC 
ACTGACATAC TGAACTGGTT TAAATGCACT CGGCACAGAG TGAGCTACCG GACAGCCTAC 
CGCCATGGGG AGAAAACCAT GTACAGACGC AAATCCCAGT GTTGCCCCGG ATTTTATGAA 
AGTCGAGACG TGTGTGTCCC TCACTGCGCT GATAAATGCG TCCACGGTCG CTGCATCGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGGACCA ACTGTAGCAG TGCTTGTGAC 
GGTGATCACT GGGGACCTCA CTGCAGCAGC CGGTGTCAGT GCAAAAACAG AGCTTTGTGT 
AACCCCATCA CCGGAGCTTG TCACTGCGCC GCGGGCTACC GGGGATGGCG CTGTGAGGAC 
CGTTGTGAAC AGGGCACCTA CGGTAACGAC TGTCATCAAA GGTGCCAGTG TCAGAATGGA 
GCGACCTGTG ACCACATCAC TGGGGAATGT CGTTGTTCAC CTGGATACAC TGGAGCCTTC 
TGTGAGGATC TGTGTCCTCC TGGCAAACAT GGCCCACAGT GTGAGCAGAG ATGTCCCTGC 
CAAAATGGAG GCGTGTGCCA TCATGTCACT GGAGAGTGCT CCTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC CTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGTCAGTGCC ACAATGGAGG AGCGTGTGAT GCTGCCACAG GCCAATGTCA CTGCAGCCCA 
GGATACACAG GGGAACGGTG TCAGGATGAA TGTCCTGTTG GAACCTATGG AGTTCGCTGT 
GCTGAGACCT GCAGGTGTGT TAATGGAGGG AAGTGTTACC ATGTGAGCGG CACATGCCTA 
TGCGAAGCAG GTTTTTCAGG TGAATTTTGC GAGGCTCGTC TGTGCCCTGA GGGGCTTTAC 
GGCATCAAAT GTGACAAGCG GTGCCCCTGC CACCTGGACA ATACTCATAG CTGTCATCCC 
ATGTCTGGAG AGTGTGGCTG CAAGCCGGGT TGGTCTGGAC TGTACTGTAA TGAAACATGC 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCAAAA CGGGGCTGAC 
TGTGACAGTG TGAGTGGAAG GTGTGCCTGT GCTCCAGGAT TCAAAGGAAC TGACTGCTCT 
ACTCCGTGTC CTCTGGGACG CTATGGGATA AATTGTTCTT CTCGCTGTGG CTGTAAAAAC 
GATGCTGTCT GTTCTCCTGT GGATGGATCG TGCATCTGTA AGGCAGGCTG GCACGGGGTG 
GACTGTTCCA TCAGCTGCCC CAGTGGCACA TGGGGCTTTG GCTGTAATCT AACCTGTCAG 
TGCCTCAATG GCGGTGCCTG CAACACACTG GACGGCACCT GCACCTGTGC GCCGGGATGG 
CGAGGGGAGA AATGTGAATT TCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCTGAG 
CGCTGTGACT GCAGCCATGC AGACGGATGT CACCCCACTA CCGGCCATTG CCGATGCCTC 
CCTGGATGGT CAGGTGTGCA CTGTGACAGT GTGTGTGCTG AGGGACGCTG GGGTCCTAAC 
TGCTCGCTGC CCTGCTACTG TAAAAATGGG GCTTCGTGTT CTCCCGATGA TGGCATCTGT 
GAATGCGCAC CCGGATTCCG AGGCACCACT TGCCAGAGAA TCTGCTCCCC GGGCTTTTAT 
GGACACCGCT GTAGCCAGAC ATGCCCACAG TGTGTGCACA GCAGTGGACC ATGCCACCAC 
ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACTGGCG CCCTGTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA GAACTGTGCA GGTGTTTGTA CCTGCACCAA CAACGGCACC 
TGTAACCCCA TCGACCGGTC CTGTCAGTGC TACCCAGGCT GGATTGGCAG TGACTGCTCC 
CAGCCCTGTC CACCAGCCCA CTGGGGTCCG AACTGCATCC ATACATGCAA CTGCCACAAT 
GGAGCCTTTT GCAGCGCCTA TGATGGGGAA TGCAAATGCA CTCCTGGCTG GACGGGGCTC 
TACTGCACTC AGAGATGCCC TCTGGGCTTC TATGGCAAGG ACTGTGCACT GGTATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATC TCGGGGCAGT GTACCTGCCG CACGGGATTC 
ATGGGACGGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ACGGCTATGG CTGTCGCCAG 
ATCTGCGACT GTCTGAACAA CTCCACCTGT GACCACATCA CCGGCACGTG TTACTGTAGC 
CCAGGGTGGA AAGGAGCACG ATGTGACCAA GCTGGGGTCA TCATCGTGGG CAATCTGAAC 
AGCTTAAGCA GGACCAGCAC CGCCCTTCCT GCTGACTCCT ATCAGATTGG GGCCATCGCG 
GGCATCGTGG TCCTTCTGCT TGTTGTGCTC TTCCTGCTGG CGCTGTTCGT TATCTACAGA 
CACAAGCAGA AGAGGAAGGA ATCGAGCATG CCGGCCGTGA CCTACACCCC TGCCATGAGG 
GTTATCAATG CAGACTATAC CATTGCAGAA ACCCTGCCTC ACAGCAATGG CGGAAATGCC 
AACAGCCACT ACTTCACCAA TCCCAGTTAT CACACACTTA GCCAGTGTGC CGCGTCCCCT 
CATGTGAACA ACAGGGACAG GATGACCATT GCAAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAATCC AGGGAAGAGA GGCGCTTTGG TGGACTGCAC TGGGACGTTG 
CCAGCTGACT GGAAGCAAGG AGGCTACCTC AATGAACTTG GTGCTTTTGG GCTGGACAGA 
AGCTACATGG GAAAGTCCTT AAAAGATCTG GGGAAGAATT CTGAGTACAA TTCAAGTACT 
TGTTCCCTAA GCAGCTCGGA GAACCCATAT GCCACCATTA AAGACCCTCC TGCGCTCTTG 
CCTAAAAGCT CCGAGTGTGG CTACGTGGAG ATGAAGTCGC CAGCGCGAAG AGACTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT CGAACCTACA 
GTCAGTGTGG TGCAAGGAGT ATTCAGCAAC AATGGTCATG TCACCCAAGA CCCATATGAC 
CTTCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTAAG GGACAGTTCA 
TCCTCCCCAA AGAGAGAGGA TGGTGGTGGC AGCAGCAGCA GCACCACCAC CAGCAACAGC 
AGCAACAGCA GCGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Megf10 ( XM_017600884.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456373.1 Rattus norvegicus multiple EGF-like domains 10 (Megf10), transcript variant X2, mRNA.

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CloneID	ORa04464
Clone ID Related Accession (Same CDS sequence)	XM_006254747.2 , NM_001100657.1 , XM_017600884.1
Accession Version	XM_017600884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	multiple epidermal growth factor-like domains protein 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTATTT CTTCAAGCTC CTGCCTGGGC CTCATCTGCT CACTGCTGTG CCACTGGCTG 
AGGGCAGCAT CCTCACTGAA CCTGGAAGAC CCCAATGTAT GCAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAGGAGTC TTACCCACAT CCCTTCGATC AGATTTACTA CACAAGCTGC 
ACTGACATAC TGAACTGGTT TAAATGCACT CGGCACAGAG TGAGCTACCG GACAGCCTAC 
CGCCATGGGG AGAAAACCAT GTACAGACGC AAATCCCAGT GTTGCCCCGG ATTTTATGAA 
AGTCGAGACG TGTGTGTCCC TCACTGCGCT GATAAATGCG TCCACGGTCG CTGCATCGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGGACCA ACTGTAGCAG TGCTTGTGAC 
GGTGATCACT GGGGACCTCA CTGCAGCAGC CGGTGTCAGT GCAAAAACAG AGCTTTGTGT 
AACCCCATCA CCGGAGCTTG TCACTGCGCC GCGGGCTACC GGGGATGGCG CTGTGAGGAC 
CGTTGTGAAC AGGGCACCTA CGGTAACGAC TGTCATCAAA GGTGCCAGTG TCAGAATGGA 
GCGACCTGTG ACCACATCAC TGGGGAATGT CGTTGTTCAC CTGGATACAC TGGAGCCTTC 
TGTGAGGATC TGTGTCCTCC TGGCAAACAT GGCCCACAGT GTGAGCAGAG ATGTCCCTGC 
CAAAATGGAG GCGTGTGCCA TCATGTCACT GGAGAGTGCT CCTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC CTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGTCAGTGCC ACAATGGAGG AGCGTGTGAT GCTGCCACAG GCCAATGTCA CTGCAGCCCA 
GGATACACAG GGGAACGGTG TCAGGATGAA TGTCCTGTTG GAACCTATGG AGTTCGCTGT 
GCTGAGACCT GCAGGTGTGT TAATGGAGGG AAGTGTTACC ATGTGAGCGG CACATGCCTA 
TGCGAAGCAG GTTTTTCAGG TGAATTTTGC GAGGCTCGTC TGTGCCCTGA GGGGCTTTAC 
GGCATCAAAT GTGACAAGCG GTGCCCCTGC CACCTGGACA ATACTCATAG CTGTCATCCC 
ATGTCTGGAG AGTGTGGCTG CAAGCCGGGT TGGTCTGGAC TGTACTGTAA TGAAACATGC 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCAAAA CGGGGCTGAC 
TGTGACAGTG TGAGTGGAAG GTGTGCCTGT GCTCCAGGAT TCAAAGGAAC TGACTGCTCT 
ACTCCGTGTC CTCTGGGACG CTATGGGATA AATTGTTCTT CTCGCTGTGG CTGTAAAAAC 
GATGCTGTCT GTTCTCCTGT GGATGGATCG TGCATCTGTA AGGCAGGCTG GCACGGGGTG 
GACTGTTCCA TCAGCTGCCC CAGTGGCACA TGGGGCTTTG GCTGTAATCT AACCTGTCAG 
TGCCTCAATG GCGGTGCCTG CAACACACTG GACGGCACCT GCACCTGTGC GCCGGGATGG 
CGAGGGGAGA AATGTGAATT TCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCTGAG 
CGCTGTGACT GCAGCCATGC AGACGGATGT CACCCCACTA CCGGCCATTG CCGATGCCTC 
CCTGGATGGT CAGGTGTGCA CTGTGACAGT GTGTGTGCTG AGGGACGCTG GGGTCCTAAC 
TGCTCGCTGC CCTGCTACTG TAAAAATGGG GCTTCGTGTT CTCCCGATGA TGGCATCTGT 
GAATGCGCAC CCGGATTCCG AGGCACCACT TGCCAGAGAA TCTGCTCCCC GGGCTTTTAT 
GGACACCGCT GTAGCCAGAC ATGCCCACAG TGTGTGCACA GCAGTGGACC ATGCCACCAC 
ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACTGGCG CCCTGTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA GAACTGTGCA GGTGTTTGTA CCTGCACCAA CAACGGCACC 
TGTAACCCCA TCGACCGGTC CTGTCAGTGC TACCCAGGCT GGATTGGCAG TGACTGCTCC 
CAGCCCTGTC CACCAGCCCA CTGGGGTCCG AACTGCATCC ATACATGCAA CTGCCACAAT 
GGAGCCTTTT GCAGCGCCTA TGATGGGGAA TGCAAATGCA CTCCTGGCTG GACGGGGCTC 
TACTGCACTC AGAGATGCCC TCTGGGCTTC TATGGCAAGG ACTGTGCACT GGTATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATC TCGGGGCAGT GTACCTGCCG CACGGGATTC 
ATGGGACGGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ACGGCTATGG CTGTCGCCAG 
ATCTGCGACT GTCTGAACAA CTCCACCTGT GACCACATCA CCGGCACGTG TTACTGTAGC 
CCAGGGTGGA AAGGAGCACG ATGTGACCAA GCTGGGGTCA TCATCGTGGG CAATCTGAAC 
AGCTTAAGCA GGACCAGCAC CGCCCTTCCT GCTGACTCCT ATCAGATTGG GGCCATCGCG 
GGCATCGTGG TCCTTCTGCT TGTTGTGCTC TTCCTGCTGG CGCTGTTCGT TATCTACAGA 
CACAAGCAGA AGAGGAAGGA ATCGAGCATG CCGGCCGTGA CCTACACCCC TGCCATGAGG 
GTTATCAATG CAGACTATAC CATTGCAGAA ACCCTGCCTC ACAGCAATGG CGGAAATGCC 
AACAGCCACT ACTTCACCAA TCCCAGTTAT CACACACTTA GCCAGTGTGC CGCGTCCCCT 
CATGTGAACA ACAGGGACAG GATGACCATT GCAAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAATCC AGGGAAGAGA GGCGCTTTGG TGGACTGCAC TGGGACGTTG 
CCAGCTGACT GGAAGCAAGG AGGCTACCTC AATGAACTTG GTGCTTTTGG GCTGGACAGA 
AGCTACATGG GAAAGTCCTT AAAAGATCTG GGGAAGAATT CTGAGTACAA TTCAAGTACT 
TGTTCCCTAA GCAGCTCGGA GAACCCATAT GCCACCATTA AAGACCCTCC TGCGCTCTTG 
CCTAAAAGCT CCGAGTGTGG CTACGTGGAG ATGAAGTCGC CAGCGCGAAG AGACTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT CGAACCTACA 
GTCAGTGTGG TGCAAGGAGT ATTCAGCAAC AATGGTCATG TCACCCAAGA CCCATATGAC 
CTTCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTAAG GGACAGTTCA 
TCCTCCCCAA AGAGAGAGGA TGGTGGTGGC AGCAGCAGCA GCACCACCAC CAGCAACAGC 
AGCAACAGCA GCGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456373.1
CDS	79..3516
Translation	MAISSSSCLGLICSLLCHWLRAASSLNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCTRHRVSYRTAYRHGEKTMYRRKSQCCPGFYESRDVCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACDGDHWGPHCSSRCQCKNRALCNPITGACHCAAGYRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHITGECRCSPGYTGAFCEDLCPPGKHGPQCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQECQCHNGGACDAATGQCHCSPGYTGERCQDECPVGTYGVRCAETCRCVNGGKCYHVSGTCLCEAGFSGEFCEARLCPEGLYGIKCDKRCPCHLDNTHSCHPMSGECGCKPGWSGLYCNETCSPGFYGEACQQICSCQNGADCDSVSGRCACAPGFKGTDCSTPCPLGRYGINCSSRCGCKNDAVCSPVDGSCICKAGWHGVDCSISCPSGTWGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCEFPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCLPGWSGVHCDSVCAEGRWGPNCSLPCYCKNGASCSPDDGICECAPGFRGTTCQRICSPGFYGHRCSQTCPQCVHSSGPCHHITGLCDCLPGFTGALCNEVCPSGRFGKNCAGVCTCTNNGTCNPIDRSCQCYPGWIGSDCSQPCPPAHWGPNCIHTCNCHNGAFCSAYDGECKCTPGWTGLYCTQRCPLGFYGKDCALVCQCQNGADCDHISGQCTCRTGFMGRHCEQKCPAGTYGYGCRQICDCLNNSTCDHITGTCYCSPGWKGARCDQAGVIIVGNLNSLSRTSTALPADSYQIGAIAGIVVLLLVVLFLLALFVIYRHKQKRKESSMPAVTYTPAMRVINADYTIAETLPHSNGGNANSHYFTNPSYHTLSQCAASPHVNNRDRMTIAKSKNNQLFVNLKNVNPGKRGALVDCTGTLPADWKQGGYLNELGAFGLDRSYMGKSLKDLGKNSEYNSSTCSLSSSENPYATIKDPPALLPKSSECGYVEMKSPARRDSPYAEINNSTSANRNVYEVEPTVSVVQGVFSNNGHVTQDPYDLPKNSHIPCHYDLLPVRDSSSSPKREDGGGSSSSTTTSNSSNSSE

Target ORF information:

RefSeq Version	XM_017600884.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus multiple EGF-like domains 10 (Megf10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600884.1

ORF Insert Sequence:

ATGGCTATTT CTTCAAGCTC CTGCCTGGGC CTCATCTGCT CACTGCTGTG CCACTGGCTG 
AGGGCAGCAT CCTCACTGAA CCTGGAAGAC CCCAATGTAT GCAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAGGAGTC TTACCCACAT CCCTTCGATC AGATTTACTA CACAAGCTGC 
ACTGACATAC TGAACTGGTT TAAATGCACT CGGCACAGAG TGAGCTACCG GACAGCCTAC 
CGCCATGGGG AGAAAACCAT GTACAGACGC AAATCCCAGT GTTGCCCCGG ATTTTATGAA 
AGTCGAGACG TGTGTGTCCC TCACTGCGCT GATAAATGCG TCCACGGTCG CTGCATCGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGGACCA ACTGTAGCAG TGCTTGTGAC 
GGTGATCACT GGGGACCTCA CTGCAGCAGC CGGTGTCAGT GCAAAAACAG AGCTTTGTGT 
AACCCCATCA CCGGAGCTTG TCACTGCGCC GCGGGCTACC GGGGATGGCG CTGTGAGGAC 
CGTTGTGAAC AGGGCACCTA CGGTAACGAC TGTCATCAAA GGTGCCAGTG TCAGAATGGA 
GCGACCTGTG ACCACATCAC TGGGGAATGT CGTTGTTCAC CTGGATACAC TGGAGCCTTC 
TGTGAGGATC TGTGTCCTCC TGGCAAACAT GGCCCACAGT GTGAGCAGAG ATGTCCCTGC 
CAAAATGGAG GCGTGTGCCA TCATGTCACT GGAGAGTGCT CCTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC CTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGTCAGTGCC ACAATGGAGG AGCGTGTGAT GCTGCCACAG GCCAATGTCA CTGCAGCCCA 
GGATACACAG GGGAACGGTG TCAGGATGAA TGTCCTGTTG GAACCTATGG AGTTCGCTGT 
GCTGAGACCT GCAGGTGTGT TAATGGAGGG AAGTGTTACC ATGTGAGCGG CACATGCCTA 
TGCGAAGCAG GTTTTTCAGG TGAATTTTGC GAGGCTCGTC TGTGCCCTGA GGGGCTTTAC 
GGCATCAAAT GTGACAAGCG GTGCCCCTGC CACCTGGACA ATACTCATAG CTGTCATCCC 
ATGTCTGGAG AGTGTGGCTG CAAGCCGGGT TGGTCTGGAC TGTACTGTAA TGAAACATGC 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCAAAA CGGGGCTGAC 
TGTGACAGTG TGAGTGGAAG GTGTGCCTGT GCTCCAGGAT TCAAAGGAAC TGACTGCTCT 
ACTCCGTGTC CTCTGGGACG CTATGGGATA AATTGTTCTT CTCGCTGTGG CTGTAAAAAC 
GATGCTGTCT GTTCTCCTGT GGATGGATCG TGCATCTGTA AGGCAGGCTG GCACGGGGTG 
GACTGTTCCA TCAGCTGCCC CAGTGGCACA TGGGGCTTTG GCTGTAATCT AACCTGTCAG 
TGCCTCAATG GCGGTGCCTG CAACACACTG GACGGCACCT GCACCTGTGC GCCGGGATGG 
CGAGGGGAGA AATGTGAATT TCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCTGAG 
CGCTGTGACT GCAGCCATGC AGACGGATGT CACCCCACTA CCGGCCATTG CCGATGCCTC 
CCTGGATGGT CAGGTGTGCA CTGTGACAGT GTGTGTGCTG AGGGACGCTG GGGTCCTAAC 
TGCTCGCTGC CCTGCTACTG TAAAAATGGG GCTTCGTGTT CTCCCGATGA TGGCATCTGT 
GAATGCGCAC CCGGATTCCG AGGCACCACT TGCCAGAGAA TCTGCTCCCC GGGCTTTTAT 
GGACACCGCT GTAGCCAGAC ATGCCCACAG TGTGTGCACA GCAGTGGACC ATGCCACCAC 
ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACTGGCG CCCTGTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA GAACTGTGCA GGTGTTTGTA CCTGCACCAA CAACGGCACC 
TGTAACCCCA TCGACCGGTC CTGTCAGTGC TACCCAGGCT GGATTGGCAG TGACTGCTCC 
CAGCCCTGTC CACCAGCCCA CTGGGGTCCG AACTGCATCC ATACATGCAA CTGCCACAAT 
GGAGCCTTTT GCAGCGCCTA TGATGGGGAA TGCAAATGCA CTCCTGGCTG GACGGGGCTC 
TACTGCACTC AGAGATGCCC TCTGGGCTTC TATGGCAAGG ACTGTGCACT GGTATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATC TCGGGGCAGT GTACCTGCCG CACGGGATTC 
ATGGGACGGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ACGGCTATGG CTGTCGCCAG 
ATCTGCGACT GTCTGAACAA CTCCACCTGT GACCACATCA CCGGCACGTG TTACTGTAGC 
CCAGGGTGGA AAGGAGCACG ATGTGACCAA GCTGGGGTCA TCATCGTGGG CAATCTGAAC 
AGCTTAAGCA GGACCAGCAC CGCCCTTCCT GCTGACTCCT ATCAGATTGG GGCCATCGCG 
GGCATCGTGG TCCTTCTGCT TGTTGTGCTC TTCCTGCTGG CGCTGTTCGT TATCTACAGA 
CACAAGCAGA AGAGGAAGGA ATCGAGCATG CCGGCCGTGA CCTACACCCC TGCCATGAGG 
GTTATCAATG CAGACTATAC CATTGCAGAA ACCCTGCCTC ACAGCAATGG CGGAAATGCC 
AACAGCCACT ACTTCACCAA TCCCAGTTAT CACACACTTA GCCAGTGTGC CGCGTCCCCT 
CATGTGAACA ACAGGGACAG GATGACCATT GCAAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAATCC AGGGAAGAGA GGCGCTTTGG TGGACTGCAC TGGGACGTTG 
CCAGCTGACT GGAAGCAAGG AGGCTACCTC AATGAACTTG GTGCTTTTGG GCTGGACAGA 
AGCTACATGG GAAAGTCCTT AAAAGATCTG GGGAAGAATT CTGAGTACAA TTCAAGTACT 
TGTTCCCTAA GCAGCTCGGA GAACCCATAT GCCACCATTA AAGACCCTCC TGCGCTCTTG 
CCTAAAAGCT CCGAGTGTGG CTACGTGGAG ATGAAGTCGC CAGCGCGAAG AGACTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT CGAACCTACA 
GTCAGTGTGG TGCAAGGAGT ATTCAGCAAC AATGGTCATG TCACCCAAGA CCCATATGAC 
CTTCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTAAG GGACAGTTCA 
TCCTCCCCAA AGAGAGAGGA TGGTGGTGGC AGCAGCAGCA GCACCACCAC CAGCAACAGC 
AGCAACAGCA GCGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Consequences of MEGF10 deficiency on myoblast function and Notch1 interactions.
Human molecular genetics26(15)2984-3000(2017 08)
Saha M,Mitsuhashi S,Jones MD,Manko K,Reddy HM,Bruels CC,Cho KA,Pacak CA,Draper I,Kang PB

Megf10 Is a Receptor for C1Q That Mediates Clearance of Apoptotic Cells by Astrocytes.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(19)5185-92(2016 05)
Iram T,Ramirez-Ortiz Z,Byrne MH,Coleman UA,Kingery ND,Means TK,Frenkel D,El Khoury J

MEGF10 and MEGF11 mediate homotypic interactions required for mosaic spacing of retinal neurons.
Nature483(7390)465-9(2012 Mar)
Kay JN,Chu MW,Sanes JR

Mutations in MEGF10, a regulator of satellite cell myogenesis, cause early onset myopathy, areflexia, respiratory distress and dysphagia (EMARDD).
Nature genetics43(12)1189-92(2011 Nov)
Logan CV,Lucke B,Pottinger C,Abdelhamed ZA,Parry DA,Szymanska K,Diggle CP,van Riesen A,Morgan JE,Markham G,Ellis I,Manzur AY,Markham AF,Shires M,Helliwell T,Scoto M,Hübner C,Bonthron DT,Taylor GR,Sheridan E,Muntoni F,Carr IM,Schuelke M,Johnson CA

Glial precursors clear sensory neuron corpses during development via Jedi-1, an engulfment receptor.
Nature neuroscience12(12)1534-41(2009 Dec)
Wu HH,Bellmunt E,Scheib JL,Venegas V,Burkert C,Reichardt LF,Zhou Z,Fariñas I,Carter BD

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Megf10 cDNA ORF clone, Rattus norvegicus(Norway rat)

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