Atp1a4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp1a4
Entrez Gene ID	29132
Full Name	ATPase Na+/K+ transporting subunit alpha 4
General protein information	Preferred Names ATPase Na+/K+ transporting subunit alpha 4 Names sodium/potassium-transporting ATPase subunit alpha-4 ATPase, Na+/K+ transporting, alpha 4 polypeptide Na(+)/K(+) ATPase alpha-4 subunit sodium pump subunit alpha-4 sodium/potassium-transporting ATPase subunit alpha-4-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q24
Summary	alpha subunit isoform of Na+, K(+)-ATPase, which has sodium/potassium ion exchange activity; may be involved in regulation of intracellular hydrogen ion homeostasis and sperm motility [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017598762.1	XP_017454251.1	sodium/potassium-transporting ATPase subunit alpha-4 isoform X1
NM_001271030.1	NP_001257959.1	sodium/potassium-transporting ATPase subunit alpha-4
NM_022848.3	NP_074039.1	sodium/potassium-transporting ATPase subunit alpha-4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Atp1a4	NP_001257959.1
Homo sapiens (human)	ATP1A4	NP_653300.2
Pan troglodytes (chimpanzee)	ATP1A4	XP_001171135.1
Macaca mulatta (Rhesus monkey)	ATP1A4	XP_001117376.1
Canis lupus familiaris (dog)	ATP1A4	XP_545754.3
Mus musculus (house mouse)	Atp1a4	NP_038762.1
Bos taurus (cattle)	ATP1A4	NP_001137575.1

Gene Ontology
Bibliography

Related articles in PubMed

Ouabain interactions with the α4 isoform of the sodium pump trigger non-classical steroid hormone signaling and integrin expression in spermatogenic cells.
Upmanyu N, Dietze R, Kirch U, Scheiner-Bobis G
Biochimica et biophysica acta1863(11)2809-2819(2016 11)

The α4 isoform of the Na⁺,K⁺-ATPase is tuned for changing extracellular environments.
Clausen MV, Nissen P, Poulsen H
The FEBS journal283(2)282-93(2016 Jan)

Green fluorescence protein driven by the Na,K-ATPase α4 isoform promoter is expressed only in male germ cells of mouse testis.
McDermott JP, Sánchez G, Chennathukuzhi V, Blanco G
Journal of assisted reproduction and genetics29(12)1313-25(2012 Dec)

Activity of the Na,K-ATPase α4 isoform is regulated during sperm capacitation to support sperm motility.
Jimenez T, Sánchez G, Blanco G
Journal of andrology33(5)1047-57(2012 Sep-Oct)

Na,K-ATPase alpha4 isoform is essential for sperm fertility.
Jimenez T, McDermott JP, Sánchez G, Blanco G
Proceedings of the National Academy of Sciences of the United States of America108(2)644-9(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Atp1a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp1a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa58044	XM_017598762.1 Latest version!	Rattus norvegicus ATPase Na+/K+ transporting subunit alpha 4 (Atp1a4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa12948	NM_001271030.1 Latest version!	Rattus norvegicus ATPase Na+/K+ transporting subunit alpha 4 (Atp1a4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa12948	NM_022848.3 Latest version!	Rattus norvegicus ATPase Na+/K+ transporting subunit alpha 4 (Atp1a4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp1a4 ( XM_017598762.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454251.1 Rattus norvegicus ATPase Na+/K+ transporting subunit alpha 4 (Atp1a4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58044
Clone ID Related Accession (Same CDS sequence)	XM_017598762.1
Accession Version	XM_017598762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	sodium/potassium-transporting ATPase subunit alpha-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGC CTGGAAAAGA GACAGCGGCC ACTAGTGAGC AGAAGCCAAG ACCCACCCTT 
AGGGCTTCAA ATACGAATAG ACAGCCGAAA GTGAAAAGAA GGAAGAAAGA TTTAGAGGAG 
TTGAAGAAGG AAGTGGTTAT GGACGATCAC AAGCTAACGT TGGATGAGCT GAGTGCCAAG 
TACTCTGTGG ACTTGACCAA GGGTCTTAGC GTCACAGATG CCCAGGAAAT TCTGACTCTA 
AATGGACCGA ATGTACTAAC CCCTCCTCCA ACCACTCCAG AATGGATCAA ATTCTGTAAA 
CAATTATTTG GTGGCTTCTC CCTCCTGCTG TGGACTGGTT CCTTGCTCTG CTTCCTGGCC 
TATGGCATAC ATGTATCTTA TTACCAAGAA AACGCCAACA AAGATAATCT GTACCTGGGT 
ATTGTACTAT CTGCCGTGGT CATCATCACT GGCTGCTTCT CATATTACCA GGAGGCGAAG 
AGCTCAAAGA TCATGGAGTC TTTTAAGACC ATGGTGCCTC AGCAAGCTCT GGTAATTCGA 
GATGGAGAGA AAATGCAGAT CAATGTAAGA GATGTGGTTT TGGGAGACCT AGTAGAAGTG 
AAGGGTGGAG ACCAAGTCCC TGCTGATATC AGGGTTATCG CTGCGCAAGG ATGTAAGGTA 
GACAATTCCT CCTTGACTGG GGAGTCAGAA CCACAGTCTC GTTGCCCTGA TTGCACCCAT 
GAGAACCCTC TGGAGACACG GAATATCATC TTCTTCTCCA CCAACTGCGT AGAAGGAACA 
GCCCGGGGTG TTGTGATTGC TACAGGAGAC CACACAGTAA TGGGCCGGAT TGCTTCCCTG 
ACATCAGGAT TGACAATGGG CAAGACCCCG ATTGCCACTG AAATCGAACA CTTCATCCAT 
ATAATCACAG CTGTCGCAGT CTTCCTCGGT GTCACGTTTT TTTTCCTCTC ACTTATCTTG 
GGCTATACCT GGCTGGATGC TGTCATTTTC CTTATTGGCA TCATTGTGGC CAATGTGCCA 
GAAGGGCTGC TGGCCACTGT CACTGTGTGC CTGACGCTGA CAGCCAAGCG CATGGCTCGC 
AAGAACTGCC TGGTGAAGAA CCTGGAGGCG GTGGAGACGC TGGGCTCCAC GTCCACCATC 
TGCTCGGACA AGACTGGCAC CCTCACCCAG AACCGCATGA CGGTGGCTCA TCTGTGGTTT 
GATAAGACTG TGTATGAGGC TGACACCAGC GAGGAGCAAA CCACGGGGAA AACATTTCCC 
AAGTCCTCTG ACACCTGGTT TTACCTGGCT CGAATTGCTG GCCTCTGCAA CCGGGCCGAC 
TTTAAGCCTC ATCAGGAGTC TCTTCCCATA ACTAAGCGGA CAACAACAGG GGATGCTTCT 
GAGTCAGCCC TCCTCAAATT CATTGAGCAG TCTTACAGCC CTGTGAGTGA AATGAGACAG 
AAAAACCCCA AGGTGGCAGA GATTCCTTTT AATTCTACCA ACAAGTACCA GATGTCTATT 
CACCTTCTGG AAGATAACTC AGAAGCCCAC GTGCTGCTGA TGAAAGGTGC TCCAGAAAGG 
ATCTTGGATT TTTGCTCCAG TTTCCTGCTC AATGGGCAGG AGTACCCTAT GGACGAAGAA 
ATGAAGACAG ACTTCCAGAA TGCCTACATA GAACTGGGAG GTCTGGGCGA GCGTGTGCTG 
GGGTTCTGCT TCTTGAATTT GCCTAGCAAT TTCTCTAAGG GATTCCAATT TAATACCGAA 
GAATTAAATT TTCCCATGGA GAATCTGTGT TTCGCCGGCC TCATATCTAT GATCGACCCT 
CCTCGAACGG CTGTGCCTGA TGCTGTTAGC AAGTGCCGAA GTGCAGGGAT TAAGGTAATC 
ATGGTAACAG GGGATCATCC CATCACAGCC AAAGCTATTG CCAAAAGTGT GGGCATCATT 
TCAGAAGCTA ATGAAACGGC GGAGGACATC GCTGCTCGGC TTAATATCTC CATCAGTCAA 
GTCAGTAACA AGTCTATTAA AGCCATTGTA GTGCACGGCT CAGAGCTGAA GGACATGGAC 
TCAGGTCAGC TGGACAATAT CCTCAAGTCC TACAAGGAGA TCGTGTTTGC GCGGACCTCT 
CCTCAGCAGA AGCTCATCAT CGTGGAGGGC TGTCAGAGAC TGGGAGCCAT TGTGGCAGTG 
ACTGGTGACG GGGTGAACGA CTCCCCCGCG CTGAAGAAGG CTGACATCGG CATTGCCATG 
GGCATCACTG GCTCTGATGT CTCTAAGCAG GCGGCTGACA TGATCCTTCT CGACGACAAC 
TTTGCCTCCA TTGTGACGGG CGTGGAGGAG GGTCGCCTCA TCTTTGACAA CCTGAAGAAG 
TCCATCGCCT ATACCCTGAC CAGCAACATC CCTGAGATCA CCCCCTTCCT GCTGTTCATC 
GTCTTAAGTA TACCCTTGCC TCTGGGTACC ATTACCATTC TCTGCATTGA CCTGGGTACT 
GACATGGTCC CTGCTATCTC CTTGGCTTAT GAGACACCTG AAAGTGACAT CATGAAAAGG 
CTTCCACGGA ACCCCAAAAC TGACAATCTC GTGAACGACC GTCTTATCGG CATGGCCTAT 
GGGCAGATCG GGATGATACA GGCGCTGGCT GGATTTTTCA CCTATTTCGT GATCCTGGCG 
GAGAATGGTT TTAAGCCTCT TGATCTTCTG GGTATCCGTC TATACTGGGA TGATACAAAC 
CTAAATGACC TAGAGGACAC CTATGGGCAG CAGTGGACCT ATGAGCAGCG GAAGGTGGTG 
GAGTTCACAT GCCAAACAGC CTTCTTCATC AGCATCGTGA TCGTGCAGTG GGCTGACCTC 
ATCATCTGCA AGACGCGCAG AAATTCACTC TTCAAGCAGG GCATGAAGAT AAACTGGTGG 
TTCTGCGCAC TTCCCTACAG TGTTCTCATC TTCATCTATG ATGAAGTTCG AAAACTCATC 
ATCCGCAGAC GGCCCGGTGG ATGGCTGGAG AAGGAGACGT ACTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454251.1
CDS	350..3337
Translation	MMEPGKETAATSEQKPRPTLRASNTNRQPKVKRRKKDLEELKKEVVMDDHKLTLDELSAKYSVDLTKGLSVTDAQEILTLNGPNVLTPPPTTPEWIKFCKQLFGGFSLLLWTGSLLCFLAYGIHVSYYQENANKDNLYLGIVLSAVVIITGCFSYYQEAKSSKIMESFKTMVPQQALVIRDGEKMQINVRDVVLGDLVEVKGGDQVPADIRVIAAQGCKVDNSSLTGESEPQSRCPDCTHENPLETRNIIFFSTNCVEGTARGVVIATGDHTVMGRIASLTSGLTMGKTPIATEIEHFIHIITAVAVFLGVTFFFLSLILGYTWLDAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHLWFDKTVYEADTSEEQTTGKTFPKSSDTWFYLARIAGLCNRADFKPHQESLPITKRTTTGDASESALLKFIEQSYSPVSEMRQKNPKVAEIPFNSTNKYQMSIHLLEDNSEAHVLLMKGAPERILDFCSSFLLNGQEYPMDEEMKTDFQNAYIELGGLGERVLGFCFLNLPSNFSKGFQFNTEELNFPMENLCFAGLISMIDPPRTAVPDAVSKCRSAGIKVIMVTGDHPITAKAIAKSVGIISEANETAEDIAARLNISISQVSNKSIKAIVVHGSELKDMDSGQLDNILKSYKEIVFARTSPQQKLIIVEGCQRLGAIVAVTGDGVNDSPALKKADIGIAMGITGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIVLSIPLPLGTITILCIDLGTDMVPAISLAYETPESDIMKRLPRNPKTDNLVNDRLIGMAYGQIGMIQALAGFFTYFVILAENGFKPLDLLGIRLYWDDTNLNDLEDTYGQQWTYEQRKVVEFTCQTAFFISIVIVQWADLIICKTRRNSLFKQGMKINWWFCALPYSVLIFIYDEVRKLIIRRRPGGWLEKETYY

Target ORF information:

RefSeq Version	XM_017598762.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase Na+/K+ transporting subunit alpha 4 (Atp1a4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598762.1

ORF Insert Sequence:

ATGATGGAGC CTGGAAAAGA GACAGCGGCC ACTAGTGAGC AGAAGCCAAG ACCCACCCTT 
AGGGCTTCAA ATACGAATAG ACAGCCGAAA GTGAAAAGAA GGAAGAAAGA TTTAGAGGAG 
TTGAAGAAGG AAGTGGTTAT GGACGATCAC AAGCTAACGT TGGATGAGCT GAGTGCCAAG 
TACTCTGTGG ACTTGACCAA GGGTCTTAGC GTCACAGATG CCCAGGAAAT TCTGACTCTA 
AATGGACCGA ATGTACTAAC CCCTCCTCCA ACCACTCCAG AATGGATCAA ATTCTGTAAA 
CAATTATTTG GTGGCTTCTC CCTCCTGCTG TGGACTGGTT CCTTGCTCTG CTTCCTGGCC 
TATGGCATAC ATGTATCTTA TTACCAAGAA AACGCCAACA AAGATAATCT GTACCTGGGT 
ATTGTACTAT CTGCCGTGGT CATCATCACT GGCTGCTTCT CATATTACCA GGAGGCGAAG 
AGCTCAAAGA TCATGGAGTC TTTTAAGACC ATGGTGCCTC AGCAAGCTCT GGTAATTCGA 
GATGGAGAGA AAATGCAGAT CAATGTAAGA GATGTGGTTT TGGGAGACCT AGTAGAAGTG 
AAGGGTGGAG ACCAAGTCCC TGCTGATATC AGGGTTATCG CTGCGCAAGG ATGTAAGGTA 
GACAATTCCT CCTTGACTGG GGAGTCAGAA CCACAGTCTC GTTGCCCTGA TTGCACCCAT 
GAGAACCCTC TGGAGACACG GAATATCATC TTCTTCTCCA CCAACTGCGT AGAAGGAACA 
GCCCGGGGTG TTGTGATTGC TACAGGAGAC CACACAGTAA TGGGCCGGAT TGCTTCCCTG 
ACATCAGGAT TGACAATGGG CAAGACCCCG ATTGCCACTG AAATCGAACA CTTCATCCAT 
ATAATCACAG CTGTCGCAGT CTTCCTCGGT GTCACGTTTT TTTTCCTCTC ACTTATCTTG 
GGCTATACCT GGCTGGATGC TGTCATTTTC CTTATTGGCA TCATTGTGGC CAATGTGCCA 
GAAGGGCTGC TGGCCACTGT CACTGTGTGC CTGACGCTGA CAGCCAAGCG CATGGCTCGC 
AAGAACTGCC TGGTGAAGAA CCTGGAGGCG GTGGAGACGC TGGGCTCCAC GTCCACCATC 
TGCTCGGACA AGACTGGCAC CCTCACCCAG AACCGCATGA CGGTGGCTCA TCTGTGGTTT 
GATAAGACTG TGTATGAGGC TGACACCAGC GAGGAGCAAA CCACGGGGAA AACATTTCCC 
AAGTCCTCTG ACACCTGGTT TTACCTGGCT CGAATTGCTG GCCTCTGCAA CCGGGCCGAC 
TTTAAGCCTC ATCAGGAGTC TCTTCCCATA ACTAAGCGGA CAACAACAGG GGATGCTTCT 
GAGTCAGCCC TCCTCAAATT CATTGAGCAG TCTTACAGCC CTGTGAGTGA AATGAGACAG 
AAAAACCCCA AGGTGGCAGA GATTCCTTTT AATTCTACCA ACAAGTACCA GATGTCTATT 
CACCTTCTGG AAGATAACTC AGAAGCCCAC GTGCTGCTGA TGAAAGGTGC TCCAGAAAGG 
ATCTTGGATT TTTGCTCCAG TTTCCTGCTC AATGGGCAGG AGTACCCTAT GGACGAAGAA 
ATGAAGACAG ACTTCCAGAA TGCCTACATA GAACTGGGAG GTCTGGGCGA GCGTGTGCTG 
GGGTTCTGCT TCTTGAATTT GCCTAGCAAT TTCTCTAAGG GATTCCAATT TAATACCGAA 
GAATTAAATT TTCCCATGGA GAATCTGTGT TTCGCCGGCC TCATATCTAT GATCGACCCT 
CCTCGAACGG CTGTGCCTGA TGCTGTTAGC AAGTGCCGAA GTGCAGGGAT TAAGGTAATC 
ATGGTAACAG GGGATCATCC CATCACAGCC AAAGCTATTG CCAAAAGTGT GGGCATCATT 
TCAGAAGCTA ATGAAACGGC GGAGGACATC GCTGCTCGGC TTAATATCTC CATCAGTCAA 
GTCAGTAACA AGTCTATTAA AGCCATTGTA GTGCACGGCT CAGAGCTGAA GGACATGGAC 
TCAGGTCAGC TGGACAATAT CCTCAAGTCC TACAAGGAGA TCGTGTTTGC GCGGACCTCT 
CCTCAGCAGA AGCTCATCAT CGTGGAGGGC TGTCAGAGAC TGGGAGCCAT TGTGGCAGTG 
ACTGGTGACG GGGTGAACGA CTCCCCCGCG CTGAAGAAGG CTGACATCGG CATTGCCATG 
GGCATCACTG GCTCTGATGT CTCTAAGCAG GCGGCTGACA TGATCCTTCT CGACGACAAC 
TTTGCCTCCA TTGTGACGGG CGTGGAGGAG GGTCGCCTCA TCTTTGACAA CCTGAAGAAG 
TCCATCGCCT ATACCCTGAC CAGCAACATC CCTGAGATCA CCCCCTTCCT GCTGTTCATC 
GTCTTAAGTA TACCCTTGCC TCTGGGTACC ATTACCATTC TCTGCATTGA CCTGGGTACT 
GACATGGTCC CTGCTATCTC CTTGGCTTAT GAGACACCTG AAAGTGACAT CATGAAAAGG 
CTTCCACGGA ACCCCAAAAC TGACAATCTC GTGAACGACC GTCTTATCGG CATGGCCTAT 
GGGCAGATCG GGATGATACA GGCGCTGGCT GGATTTTTCA CCTATTTCGT GATCCTGGCG 
GAGAATGGTT TTAAGCCTCT TGATCTTCTG GGTATCCGTC TATACTGGGA TGATACAAAC 
CTAAATGACC TAGAGGACAC CTATGGGCAG CAGTGGACCT ATGAGCAGCG GAAGGTGGTG 
GAGTTCACAT GCCAAACAGC CTTCTTCATC AGCATCGTGA TCGTGCAGTG GGCTGACCTC 
ATCATCTGCA AGACGCGCAG AAATTCACTC TTCAAGCAGG GCATGAAGAT AAACTGGTGG 
TTCTGCGCAC TTCCCTACAG TGTTCTCATC TTCATCTATG ATGAAGTTCG AAAACTCATC 
ATCCGCAGAC GGCCCGGTGG ATGGCTGGAG AAGGAGACGT ACTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp1a4 ( NM_001271030.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257959.1 Rattus norvegicus ATPase Na+/K+ transporting subunit alpha 4 (Atp1a4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa12948
Clone ID Related Accession (Same CDS sequence)	NM_001271030.1 , NM_022848.3
Accession Version	NM_001271030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564934400000
Organism	Rattus norvegicus(Norway rat)
Product	sodium/potassium-transporting ATPase subunit alpha-4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095300.6 and BC087015.1. Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC087015.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689604, SAMN02642708 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGGAGCCTG GAAAAGAGAC AGCGGCCACT AGTGAGCAGA AGCCAAGACC CACCCTTAGG 
GCTTCAAATA CGAATAGACA GCCGAAAGTG AAAAGAAGGA AGAAAGATTT AGAGGAGTTG 
AAGAAGGAAG TGGTTATGGA CGATCACAAG CTAACGTTGG ATGAGCTGAG TGCCAAGTAC 
TCTGTGGACT TGACCAAGGG TCTTAGCGTC ACAGATGCCC AGGAAATTCT GACTCTAAAT 
GGACCGAATG TACTAACCCC TCCTCCAACC ACTCCAGAAT GGATCAAATT CTGTAAACAA 
TTATTTGGTG GCTTCTCCCT CCTGCTGTGG ACTGGTTCCT TGCTCTGCTT CCTGGCCTAT 
GGCATACATG TATCTTATTA CCAAGAAAAC GCCAACAAAG ATAATCTGTA CCTGGGTATT 
GTACTATCTG CCGTGGTCAT CATCACTGGC TGCTTCTCAT ATTACCAGGA GGCGAAGAGC 
TCAAAGATCA TGGAGTCTTT TAAGACCATG GTGCCTCAGC AAGCTCTGGT AATTCGAGAT 
GGAGAGAAAA TGCAGATCAA TGTAAGAGAT GTGGTTTTGG GAGACCTAGT AGAAGTGAAG 
GGTGGAGACC AAGTCCCTGC TGATATCAGG GTTATCGCTG CGCAAGGATG TAAGGTAGAC 
AATTCCTCCT TGACTGGGGA GTCAGAACCA CAGTCTCGTT GCCCTGATTG CACCCATGAG 
AACCCTCTGG AGACACGGAA TATCATCTTC TTCTCCACCA ACTGCGTAGA AGGAACAGCC 
CGGGGTGTTG TGATTGCTAC AGGAGACCAC ACAGTAATGG GCCGGATTGC TTCCCTGACA 
TCAGGATTGA CAATGGGCAA GACCCCGATT GCCACTGAAA TCGAACACTT CATCCATATA 
ATCACAGCTG TCGCAGTCTT CCTCGGTGTC ACGTTTTTTT TCCTCTCACT TATCTTGGGC 
TATACCTGGC TGGATGCTGT CATTTTCCTT ATTGGCATCA TTGTGGCCAA TGTGCCAGAA 
GGGCTGCTGG CCACTGTCAC TGTGTGCCTG ACGCTGACAG CCAAGCGCAT GGCTCGCAAG 
AACTGCCTGG TGAAGAACCT GGAGGCGGTG GAGACGCTGG GCTCCACGTC CACCATCTGC 
TCGGACAAGA CTGGCACCCT CACCCAGAAC CGCATGACGG TGGCTCATCT GTGGTTTGAT 
AAGACTGTGT ATGAGGCTGA CACCAGCGAG GAGCAAACCA CGGGGAAAAC ATTTCCCAAG 
TCCTCTGACA CCTGGTTTTA CCTGGCTCGA ATTGCTGGCC TCTGCAACCG GGCCGACTTT 
AAGCCTCATC AGGAGTCTCT TCCCATAACT AAGCGGACAA CAACAGGGGA TGCTTCTGAG 
TCAGCCCTCC TCAAATTCAT TGAGCAGTCT TACAGCCCTG TGAGTGAAAT GAGACAGAAA 
AACCCCAAGG TGGCAGAGAT TCCTTTTAAT TCTACCAACA AGTACCAGAT GTCTATTCAC 
CTTCTGGAAG ATAACTCAGA AGCCCACGTG CTGCTGATGA AAGGTGCTCC AGAAAGGATC 
TTGGATTTTT GCTCCAGTTT CCTGCTCAAT GGGCAGGAGT ACCCTATGGA CGAAGAAATG 
AAGACAGACT TCCAGAATGC CTACATAGAA CTGGGAGGTC TGGGCGAGCG TGTGCTGGGG 
TTCTGCTTCT TGAATTTGCC TAGCAATTTC TCTAAGGGAT TCCAATTTAA TACCGAAGAA 
TTAAATTTTC CCATGGAGAA TCTGTGTTTC GCCGGCCTCA TATCTATGAT CGACCCTCCT 
CGAACGGCTG TGCCTGATGC TGTTAGCAAG TGCCGAAGTG CAGGGATTAA GGTAATCATG 
GTAACAGGGG ATCATCCCAT CACAGCCAAA GCTATTGCCA AAAGTGTGGG CATCATTTCA 
GAAGCTAATG AAACGGCGGA GGACATCGCT GCTCGGCTTA ATATCTCCAT CAGTCAAGTC 
AGTAACAAGT CTATTAAAGC CATTGTAGTG CACGGCTCAG AGCTGAAGGA CATGGACTCA 
GGTCAGCTGG ACAATATCCT CAAGTCCTAC AAGGAGATCG TGTTTGCGCG GACCTCTCCT 
CAGCAGAAGC TCATCATCGT GGAGGGCTGT CAGAGACTGG GAGCCATTGT GGCAGTGACT 
GGTGACGGGG TGAACGACTC CCCCGCGCTG AAGAAGGCTG ACATCGGCAT TGCCATGGGC 
ATCACTGGCT CTGATGTCTC TAAGCAGGCG GCTGACATGA TCCTTCTCGA CGACAACTTT 
GCCTCCATTG TGACGGGCGT GGAGGAGGGT CGCCTCATCT TTGACAACCT GAAGAAGTCC 
ATCGCCTATA CCCTGACCAG CAACATCCCT GAGATCACCC CCTTCCTGCT GTTCATCGTC 
TTAAGTATAC CCTTGCCTCT GGGTACCATT ACCATTCTCT GCATTGACCT GGGTACTGAC 
ATGGTCCCTG CTATCTCCTT GGCTTATGAG ACACCTGAAA GTGACATCAT GAAAAGGCTT 
CCACGGAACC CCAAAACTGA CAATCTCGTG AACGACCGTC TTATCGGCAT GGCCTATGGG 
CAGATCGGGA TGATACAGGC GCTGGCTGGA TTTTTCACCT ATTTCGTGAT CCTGGCGGAG 
AATGGTTTTA AGCCTCTTGA TCTTCTGGGT ATCCGTCTAT ACTGGGATGA TACAAACCTA 
AATGACCTAG AGGACACCTA TGGGCAGCAG TGGACCTATG AGCAGCGGAA GGTGGTGGAG 
TTCACATGCC AAACAGCCTT CTTCATCAGC ATCGTGATCG TGCAGTGGGC TGACCTCATC 
ATCTGCAAGA CGCGCAGAAA TTCACTCTTC AAGCAGGGCA TGAAAAACAA AGTCCTAATA 
TTTGGACTCC TTGAGGAGAC GATCCTGGCA GCTTGCCTGT CCTACATTCC AGGCATGGAT 
GTAGCCCTGA GAATGTACCC ACTCAAGATA AACTGGTGGT TCTGCGCACT TCCCTACAGT 
GTTCTCATCT TCATCTATGA TGAAGTTCGA AAACTCATCA TCCGCAGACG GCCCGGTGGA 
TGGCTGGAGA AGGAGACGTA CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257959.1
CDS	90..3176
Translation	MEPGKETAATSEQKPRPTLRASNTNRQPKVKRRKKDLEELKKEVVMDDHKLTLDELSAKYSVDLTKGLSVTDAQEILTLNGPNVLTPPPTTPEWIKFCKQLFGGFSLLLWTGSLLCFLAYGIHVSYYQENANKDNLYLGIVLSAVVIITGCFSYYQEAKSSKIMESFKTMVPQQALVIRDGEKMQINVRDVVLGDLVEVKGGDQVPADIRVIAAQGCKVDNSSLTGESEPQSRCPDCTHENPLETRNIIFFSTNCVEGTARGVVIATGDHTVMGRIASLTSGLTMGKTPIATEIEHFIHIITAVAVFLGVTFFFLSLILGYTWLDAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHLWFDKTVYEADTSEEQTTGKTFPKSSDTWFYLARIAGLCNRADFKPHQESLPITKRTTTGDASESALLKFIEQSYSPVSEMRQKNPKVAEIPFNSTNKYQMSIHLLEDNSEAHVLLMKGAPERILDFCSSFLLNGQEYPMDEEMKTDFQNAYIELGGLGERVLGFCFLNLPSNFSKGFQFNTEELNFPMENLCFAGLISMIDPPRTAVPDAVSKCRSAGIKVIMVTGDHPITAKAIAKSVGIISEANETAEDIAARLNISISQVSNKSIKAIVVHGSELKDMDSGQLDNILKSYKEIVFARTSPQQKLIIVEGCQRLGAIVAVTGDGVNDSPALKKADIGIAMGITGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIVLSIPLPLGTITILCIDLGTDMVPAISLAYETPESDIMKRLPRNPKTDNLVNDRLIGMAYGQIGMIQALAGFFTYFVILAENGFKPLDLLGIRLYWDDTNLNDLEDTYGQQWTYEQRKVVEFTCQTAFFISIVIVQWADLIICKTRRNSLFKQGMKNKVLIFGLLEETILAACLSYIPGMDVALRMYPLKINWWFCALPYSVLIFIYDEVRKLIIRRRPGGWLEKETYY

Target ORF information:

RefSeq Version	NM_001271030.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase Na+/K+ transporting subunit alpha 4 (Atp1a4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271030.1

ORF Insert Sequence:

ATGGAGCCTG GAAAAGAGAC AGCGGCCACT AGTGAGCAGA AGCCAAGACC CACCCTTAGG 
GCTTCAAATA CGAATAGACA GCCGAAAGTG AAAAGAAGGA AGAAAGATTT AGAGGAGTTG 
AAGAAGGAAG TGGTTATGGA CGATCACAAG CTAACGTTGG ATGAGCTGAG TGCCAAGTAC 
TCTGTGGACT TGACCAAGGG TCTTAGCGTC ACAGATGCCC AGGAAATTCT GACTCTAAAT 
GGACCGAATG TACTAACCCC TCCTCCAACC ACTCCAGAAT GGATCAAATT CTGTAAACAA 
TTATTTGGTG GCTTCTCCCT CCTGCTGTGG ACTGGTTCCT TGCTCTGCTT CCTGGCCTAT 
GGCATACATG TATCTTATTA CCAAGAAAAC GCCAACAAAG ATAATCTGTA CCTGGGTATT 
GTACTATCTG CCGTGGTCAT CATCACTGGC TGCTTCTCAT ATTACCAGGA GGCGAAGAGC 
TCAAAGATCA TGGAGTCTTT TAAGACCATG GTGCCTCAGC AAGCTCTGGT AATTCGAGAT 
GGAGAGAAAA TGCAGATCAA TGTAAGAGAT GTGGTTTTGG GAGACCTAGT AGAAGTGAAG 
GGTGGAGACC AAGTCCCTGC TGATATCAGG GTTATCGCTG CGCAAGGATG TAAGGTAGAC 
AATTCCTCCT TGACTGGGGA GTCAGAACCA CAGTCTCGTT GCCCTGATTG CACCCATGAG 
AACCCTCTGG AGACACGGAA TATCATCTTC TTCTCCACCA ACTGCGTAGA AGGAACAGCC 
CGGGGTGTTG TGATTGCTAC AGGAGACCAC ACAGTAATGG GCCGGATTGC TTCCCTGACA 
TCAGGATTGA CAATGGGCAA GACCCCGATT GCCACTGAAA TCGAACACTT CATCCATATA 
ATCACAGCTG TCGCAGTCTT CCTCGGTGTC ACGTTTTTTT TCCTCTCACT TATCTTGGGC 
TATACCTGGC TGGATGCTGT CATTTTCCTT ATTGGCATCA TTGTGGCCAA TGTGCCAGAA 
GGGCTGCTGG CCACTGTCAC TGTGTGCCTG ACGCTGACAG CCAAGCGCAT GGCTCGCAAG 
AACTGCCTGG TGAAGAACCT GGAGGCGGTG GAGACGCTGG GCTCCACGTC CACCATCTGC 
TCGGACAAGA CTGGCACCCT CACCCAGAAC CGCATGACGG TGGCTCATCT GTGGTTTGAT 
AAGACTGTGT ATGAGGCTGA CACCAGCGAG GAGCAAACCA CGGGGAAAAC ATTTCCCAAG 
TCCTCTGACA CCTGGTTTTA CCTGGCTCGA ATTGCTGGCC TCTGCAACCG GGCCGACTTT 
AAGCCTCATC AGGAGTCTCT TCCCATAACT AAGCGGACAA CAACAGGGGA TGCTTCTGAG 
TCAGCCCTCC TCAAATTCAT TGAGCAGTCT TACAGCCCTG TGAGTGAAAT GAGACAGAAA 
AACCCCAAGG TGGCAGAGAT TCCTTTTAAT TCTACCAACA AGTACCAGAT GTCTATTCAC 
CTTCTGGAAG ATAACTCAGA AGCCCACGTG CTGCTGATGA AAGGTGCTCC AGAAAGGATC 
TTGGATTTTT GCTCCAGTTT CCTGCTCAAT GGGCAGGAGT ACCCTATGGA CGAAGAAATG 
AAGACAGACT TCCAGAATGC CTACATAGAA CTGGGAGGTC TGGGCGAGCG TGTGCTGGGG 
TTCTGCTTCT TGAATTTGCC TAGCAATTTC TCTAAGGGAT TCCAATTTAA TACCGAAGAA 
TTAAATTTTC CCATGGAGAA TCTGTGTTTC GCCGGCCTCA TATCTATGAT CGACCCTCCT 
CGAACGGCTG TGCCTGATGC TGTTAGCAAG TGCCGAAGTG CAGGGATTAA GGTAATCATG 
GTAACAGGGG ATCATCCCAT CACAGCCAAA GCTATTGCCA AAAGTGTGGG CATCATTTCA 
GAAGCTAATG AAACGGCGGA GGACATCGCT GCTCGGCTTA ATATCTCCAT CAGTCAAGTC 
AGTAACAAGT CTATTAAAGC CATTGTAGTG CACGGCTCAG AGCTGAAGGA CATGGACTCA 
GGTCAGCTGG ACAATATCCT CAAGTCCTAC AAGGAGATCG TGTTTGCGCG GACCTCTCCT 
CAGCAGAAGC TCATCATCGT GGAGGGCTGT CAGAGACTGG GAGCCATTGT GGCAGTGACT 
GGTGACGGGG TGAACGACTC CCCCGCGCTG AAGAAGGCTG ACATCGGCAT TGCCATGGGC 
ATCACTGGCT CTGATGTCTC TAAGCAGGCG GCTGACATGA TCCTTCTCGA CGACAACTTT 
GCCTCCATTG TGACGGGCGT GGAGGAGGGT CGCCTCATCT TTGACAACCT GAAGAAGTCC 
ATCGCCTATA CCCTGACCAG CAACATCCCT GAGATCACCC CCTTCCTGCT GTTCATCGTC 
TTAAGTATAC CCTTGCCTCT GGGTACCATT ACCATTCTCT GCATTGACCT GGGTACTGAC 
ATGGTCCCTG CTATCTCCTT GGCTTATGAG ACACCTGAAA GTGACATCAT GAAAAGGCTT 
CCACGGAACC CCAAAACTGA CAATCTCGTG AACGACCGTC TTATCGGCAT GGCCTATGGG 
CAGATCGGGA TGATACAGGC GCTGGCTGGA TTTTTCACCT ATTTCGTGAT CCTGGCGGAG 
AATGGTTTTA AGCCTCTTGA TCTTCTGGGT ATCCGTCTAT ACTGGGATGA TACAAACCTA 
AATGACCTAG AGGACACCTA TGGGCAGCAG TGGACCTATG AGCAGCGGAA GGTGGTGGAG 
TTCACATGCC AAACAGCCTT CTTCATCAGC ATCGTGATCG TGCAGTGGGC TGACCTCATC 
ATCTGCAAGA CGCGCAGAAA TTCACTCTTC AAGCAGGGCA TGAAAAACAA AGTCCTAATA 
TTTGGACTCC TTGAGGAGAC GATCCTGGCA GCTTGCCTGT CCTACATTCC AGGCATGGAT 
GTAGCCCTGA GAATGTACCC ACTCAAGATA AACTGGTGGT TCTGCGCACT TCCCTACAGT 
GTTCTCATCT TCATCTATGA TGAAGTTCGA AAACTCATCA TCCGCAGACG GCCCGGTGGA 
TGGCTGGAGA AGGAGACGTA CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp1a4 ( NM_022848.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_074039.1 Rattus norvegicus ATPase Na+/K+ transporting subunit alpha 4 (Atp1a4), transcript variant 1, mRNA.

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CloneID	ORa12948
Clone ID Related Accession (Same CDS sequence)	NM_001271030.1 , NM_022848.3
Accession Version	NM_022848.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564848000000
Organism	Rattus norvegicus(Norway rat)
Product	sodium/potassium-transporting ATPase subunit alpha-4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095300.6. On Sep 4, 2012 this sequence version replaced NM_022848.2. Transcript Variant: This variant (1) is the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U15176.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689604, SAMN02642708 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGGAGCCTG GAAAAGAGAC AGCGGCCACT AGTGAGCAGA AGCCAAGACC CACCCTTAGG 
GCTTCAAATA CGAATAGACA GCCGAAAGTG AAAAGAAGGA AGAAAGATTT AGAGGAGTTG 
AAGAAGGAAG TGGTTATGGA CGATCACAAG CTAACGTTGG ATGAGCTGAG TGCCAAGTAC 
TCTGTGGACT TGACCAAGGG TCTTAGCGTC ACAGATGCCC AGGAAATTCT GACTCTAAAT 
GGACCGAATG TACTAACCCC TCCTCCAACC ACTCCAGAAT GGATCAAATT CTGTAAACAA 
TTATTTGGTG GCTTCTCCCT CCTGCTGTGG ACTGGTTCCT TGCTCTGCTT CCTGGCCTAT 
GGCATACATG TATCTTATTA CCAAGAAAAC GCCAACAAAG ATAATCTGTA CCTGGGTATT 
GTACTATCTG CCGTGGTCAT CATCACTGGC TGCTTCTCAT ATTACCAGGA GGCGAAGAGC 
TCAAAGATCA TGGAGTCTTT TAAGACCATG GTGCCTCAGC AAGCTCTGGT AATTCGAGAT 
GGAGAGAAAA TGCAGATCAA TGTAAGAGAT GTGGTTTTGG GAGACCTAGT AGAAGTGAAG 
GGTGGAGACC AAGTCCCTGC TGATATCAGG GTTATCGCTG CGCAAGGATG TAAGGTAGAC 
AATTCCTCCT TGACTGGGGA GTCAGAACCA CAGTCTCGTT GCCCTGATTG CACCCATGAG 
AACCCTCTGG AGACACGGAA TATCATCTTC TTCTCCACCA ACTGCGTAGA AGGAACAGCC 
CGGGGTGTTG TGATTGCTAC AGGAGACCAC ACAGTAATGG GCCGGATTGC TTCCCTGACA 
TCAGGATTGA CAATGGGCAA GACCCCGATT GCCACTGAAA TCGAACACTT CATCCATATA 
ATCACAGCTG TCGCAGTCTT CCTCGGTGTC ACGTTTTTTT TCCTCTCACT TATCTTGGGC 
TATACCTGGC TGGATGCTGT CATTTTCCTT ATTGGCATCA TTGTGGCCAA TGTGCCAGAA 
GGGCTGCTGG CCACTGTCAC TGTGTGCCTG ACGCTGACAG CCAAGCGCAT GGCTCGCAAG 
AACTGCCTGG TGAAGAACCT GGAGGCGGTG GAGACGCTGG GCTCCACGTC CACCATCTGC 
TCGGACAAGA CTGGCACCCT CACCCAGAAC CGCATGACGG TGGCTCATCT GTGGTTTGAT 
AAGACTGTGT ATGAGGCTGA CACCAGCGAG GAGCAAACCA CGGGGAAAAC ATTTCCCAAG 
TCCTCTGACA CCTGGTTTTA CCTGGCTCGA ATTGCTGGCC TCTGCAACCG GGCCGACTTT 
AAGCCTCATC AGGAGTCTCT TCCCATAACT AAGCGGACAA CAACAGGGGA TGCTTCTGAG 
TCAGCCCTCC TCAAATTCAT TGAGCAGTCT TACAGCCCTG TGAGTGAAAT GAGACAGAAA 
AACCCCAAGG TGGCAGAGAT TCCTTTTAAT TCTACCAACA AGTACCAGAT GTCTATTCAC 
CTTCTGGAAG ATAACTCAGA AGCCCACGTG CTGCTGATGA AAGGTGCTCC AGAAAGGATC 
TTGGATTTTT GCTCCAGTTT CCTGCTCAAT GGGCAGGAGT ACCCTATGGA CGAAGAAATG 
AAGACAGACT TCCAGAATGC CTACATAGAA CTGGGAGGTC TGGGCGAGCG TGTGCTGGGG 
TTCTGCTTCT TGAATTTGCC TAGCAATTTC TCTAAGGGAT TCCAATTTAA TACCGAAGAA 
TTAAATTTTC CCATGGAGAA TCTGTGTTTC GCCGGCCTCA TATCTATGAT CGACCCTCCT 
CGAACGGCTG TGCCTGATGC TGTTAGCAAG TGCCGAAGTG CAGGGATTAA GGTAATCATG 
GTAACAGGGG ATCATCCCAT CACAGCCAAA GCTATTGCCA AAAGTGTGGG CATCATTTCA 
GAAGCTAATG AAACGGCGGA GGACATCGCT GCTCGGCTTA ATATCTCCAT CAGTCAAGTC 
AGTAACAAGT CTATTAAAGC CATTGTAGTG CACGGCTCAG AGCTGAAGGA CATGGACTCA 
GGTCAGCTGG ACAATATCCT CAAGTCCTAC AAGGAGATCG TGTTTGCGCG GACCTCTCCT 
CAGCAGAAGC TCATCATCGT GGAGGGCTGT CAGAGACTGG GAGCCATTGT GGCAGTGACT 
GGTGACGGGG TGAACGACTC CCCCGCGCTG AAGAAGGCTG ACATCGGCAT TGCCATGGGC 
ATCACTGGCT CTGATGTCTC TAAGCAGGCG GCTGACATGA TCCTTCTCGA CGACAACTTT 
GCCTCCATTG TGACGGGCGT GGAGGAGGGT CGCCTCATCT TTGACAACCT GAAGAAGTCC 
ATCGCCTATA CCCTGACCAG CAACATCCCT GAGATCACCC CCTTCCTGCT GTTCATCGTC 
TTAAGTATAC CCTTGCCTCT GGGTACCATT ACCATTCTCT GCATTGACCT GGGTACTGAC 
ATGGTCCCTG CTATCTCCTT GGCTTATGAG ACACCTGAAA GTGACATCAT GAAAAGGCTT 
CCACGGAACC CCAAAACTGA CAATCTCGTG AACGACCGTC TTATCGGCAT GGCCTATGGG 
CAGATCGGGA TGATACAGGC GCTGGCTGGA TTTTTCACCT ATTTCGTGAT CCTGGCGGAG 
AATGGTTTTA AGCCTCTTGA TCTTCTGGGT ATCCGTCTAT ACTGGGATGA TACAAACCTA 
AATGACCTAG AGGACACCTA TGGGCAGCAG TGGACCTATG AGCAGCGGAA GGTGGTGGAG 
TTCACATGCC AAACAGCCTT CTTCATCAGC ATCGTGATCG TGCAGTGGGC TGACCTCATC 
ATCTGCAAGA CGCGCAGAAA TTCACTCTTC AAGCAGGGCA TGAAAAACAA AGTCCTAATA 
TTTGGACTCC TTGAGGAGAC GATCCTGGCA GCTTGCCTGT CCTACATTCC AGGCATGGAT 
GTAGCCCTGA GAATGTACCC ACTCAAGATA AACTGGTGGT TCTGCGCACT TCCCTACAGT 
GTTCTCATCT TCATCTATGA TGAAGTTCGA AAACTCATCA TCCGCAGACG GCCCGGTGGA 
TGGCTGGAGA AGGAGACGTA CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_074039.1
CDS	93..3179
Translation	MEPGKETAATSEQKPRPTLRASNTNRQPKVKRRKKDLEELKKEVVMDDHKLTLDELSAKYSVDLTKGLSVTDAQEILTLNGPNVLTPPPTTPEWIKFCKQLFGGFSLLLWTGSLLCFLAYGIHVSYYQENANKDNLYLGIVLSAVVIITGCFSYYQEAKSSKIMESFKTMVPQQALVIRDGEKMQINVRDVVLGDLVEVKGGDQVPADIRVIAAQGCKVDNSSLTGESEPQSRCPDCTHENPLETRNIIFFSTNCVEGTARGVVIATGDHTVMGRIASLTSGLTMGKTPIATEIEHFIHIITAVAVFLGVTFFFLSLILGYTWLDAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHLWFDKTVYEADTSEEQTTGKTFPKSSDTWFYLARIAGLCNRADFKPHQESLPITKRTTTGDASESALLKFIEQSYSPVSEMRQKNPKVAEIPFNSTNKYQMSIHLLEDNSEAHVLLMKGAPERILDFCSSFLLNGQEYPMDEEMKTDFQNAYIELGGLGERVLGFCFLNLPSNFSKGFQFNTEELNFPMENLCFAGLISMIDPPRTAVPDAVSKCRSAGIKVIMVTGDHPITAKAIAKSVGIISEANETAEDIAARLNISISQVSNKSIKAIVVHGSELKDMDSGQLDNILKSYKEIVFARTSPQQKLIIVEGCQRLGAIVAVTGDGVNDSPALKKADIGIAMGITGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIVLSIPLPLGTITILCIDLGTDMVPAISLAYETPESDIMKRLPRNPKTDNLVNDRLIGMAYGQIGMIQALAGFFTYFVILAENGFKPLDLLGIRLYWDDTNLNDLEDTYGQQWTYEQRKVVEFTCQTAFFISIVIVQWADLIICKTRRNSLFKQGMKNKVLIFGLLEETILAACLSYIPGMDVALRMYPLKINWWFCALPYSVLIFIYDEVRKLIIRRRPGGWLEKETYY

Target ORF information:

RefSeq Version	NM_022848.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase Na+/K+ transporting subunit alpha 4 (Atp1a4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022848.3

ORF Insert Sequence:

ATGGAGCCTG GAAAAGAGAC AGCGGCCACT AGTGAGCAGA AGCCAAGACC CACCCTTAGG 
GCTTCAAATA CGAATAGACA GCCGAAAGTG AAAAGAAGGA AGAAAGATTT AGAGGAGTTG 
AAGAAGGAAG TGGTTATGGA CGATCACAAG CTAACGTTGG ATGAGCTGAG TGCCAAGTAC 
TCTGTGGACT TGACCAAGGG TCTTAGCGTC ACAGATGCCC AGGAAATTCT GACTCTAAAT 
GGACCGAATG TACTAACCCC TCCTCCAACC ACTCCAGAAT GGATCAAATT CTGTAAACAA 
TTATTTGGTG GCTTCTCCCT CCTGCTGTGG ACTGGTTCCT TGCTCTGCTT CCTGGCCTAT 
GGCATACATG TATCTTATTA CCAAGAAAAC GCCAACAAAG ATAATCTGTA CCTGGGTATT 
GTACTATCTG CCGTGGTCAT CATCACTGGC TGCTTCTCAT ATTACCAGGA GGCGAAGAGC 
TCAAAGATCA TGGAGTCTTT TAAGACCATG GTGCCTCAGC AAGCTCTGGT AATTCGAGAT 
GGAGAGAAAA TGCAGATCAA TGTAAGAGAT GTGGTTTTGG GAGACCTAGT AGAAGTGAAG 
GGTGGAGACC AAGTCCCTGC TGATATCAGG GTTATCGCTG CGCAAGGATG TAAGGTAGAC 
AATTCCTCCT TGACTGGGGA GTCAGAACCA CAGTCTCGTT GCCCTGATTG CACCCATGAG 
AACCCTCTGG AGACACGGAA TATCATCTTC TTCTCCACCA ACTGCGTAGA AGGAACAGCC 
CGGGGTGTTG TGATTGCTAC AGGAGACCAC ACAGTAATGG GCCGGATTGC TTCCCTGACA 
TCAGGATTGA CAATGGGCAA GACCCCGATT GCCACTGAAA TCGAACACTT CATCCATATA 
ATCACAGCTG TCGCAGTCTT CCTCGGTGTC ACGTTTTTTT TCCTCTCACT TATCTTGGGC 
TATACCTGGC TGGATGCTGT CATTTTCCTT ATTGGCATCA TTGTGGCCAA TGTGCCAGAA 
GGGCTGCTGG CCACTGTCAC TGTGTGCCTG ACGCTGACAG CCAAGCGCAT GGCTCGCAAG 
AACTGCCTGG TGAAGAACCT GGAGGCGGTG GAGACGCTGG GCTCCACGTC CACCATCTGC 
TCGGACAAGA CTGGCACCCT CACCCAGAAC CGCATGACGG TGGCTCATCT GTGGTTTGAT 
AAGACTGTGT ATGAGGCTGA CACCAGCGAG GAGCAAACCA CGGGGAAAAC ATTTCCCAAG 
TCCTCTGACA CCTGGTTTTA CCTGGCTCGA ATTGCTGGCC TCTGCAACCG GGCCGACTTT 
AAGCCTCATC AGGAGTCTCT TCCCATAACT AAGCGGACAA CAACAGGGGA TGCTTCTGAG 
TCAGCCCTCC TCAAATTCAT TGAGCAGTCT TACAGCCCTG TGAGTGAAAT GAGACAGAAA 
AACCCCAAGG TGGCAGAGAT TCCTTTTAAT TCTACCAACA AGTACCAGAT GTCTATTCAC 
CTTCTGGAAG ATAACTCAGA AGCCCACGTG CTGCTGATGA AAGGTGCTCC AGAAAGGATC 
TTGGATTTTT GCTCCAGTTT CCTGCTCAAT GGGCAGGAGT ACCCTATGGA CGAAGAAATG 
AAGACAGACT TCCAGAATGC CTACATAGAA CTGGGAGGTC TGGGCGAGCG TGTGCTGGGG 
TTCTGCTTCT TGAATTTGCC TAGCAATTTC TCTAAGGGAT TCCAATTTAA TACCGAAGAA 
TTAAATTTTC CCATGGAGAA TCTGTGTTTC GCCGGCCTCA TATCTATGAT CGACCCTCCT 
CGAACGGCTG TGCCTGATGC TGTTAGCAAG TGCCGAAGTG CAGGGATTAA GGTAATCATG 
GTAACAGGGG ATCATCCCAT CACAGCCAAA GCTATTGCCA AAAGTGTGGG CATCATTTCA 
GAAGCTAATG AAACGGCGGA GGACATCGCT GCTCGGCTTA ATATCTCCAT CAGTCAAGTC 
AGTAACAAGT CTATTAAAGC CATTGTAGTG CACGGCTCAG AGCTGAAGGA CATGGACTCA 
GGTCAGCTGG ACAATATCCT CAAGTCCTAC AAGGAGATCG TGTTTGCGCG GACCTCTCCT 
CAGCAGAAGC TCATCATCGT GGAGGGCTGT CAGAGACTGG GAGCCATTGT GGCAGTGACT 
GGTGACGGGG TGAACGACTC CCCCGCGCTG AAGAAGGCTG ACATCGGCAT TGCCATGGGC 
ATCACTGGCT CTGATGTCTC TAAGCAGGCG GCTGACATGA TCCTTCTCGA CGACAACTTT 
GCCTCCATTG TGACGGGCGT GGAGGAGGGT CGCCTCATCT TTGACAACCT GAAGAAGTCC 
ATCGCCTATA CCCTGACCAG CAACATCCCT GAGATCACCC CCTTCCTGCT GTTCATCGTC 
TTAAGTATAC CCTTGCCTCT GGGTACCATT ACCATTCTCT GCATTGACCT GGGTACTGAC 
ATGGTCCCTG CTATCTCCTT GGCTTATGAG ACACCTGAAA GTGACATCAT GAAAAGGCTT 
CCACGGAACC CCAAAACTGA CAATCTCGTG AACGACCGTC TTATCGGCAT GGCCTATGGG 
CAGATCGGGA TGATACAGGC GCTGGCTGGA TTTTTCACCT ATTTCGTGAT CCTGGCGGAG 
AATGGTTTTA AGCCTCTTGA TCTTCTGGGT ATCCGTCTAT ACTGGGATGA TACAAACCTA 
AATGACCTAG AGGACACCTA TGGGCAGCAG TGGACCTATG AGCAGCGGAA GGTGGTGGAG 
TTCACATGCC AAACAGCCTT CTTCATCAGC ATCGTGATCG TGCAGTGGGC TGACCTCATC 
ATCTGCAAGA CGCGCAGAAA TTCACTCTTC AAGCAGGGCA TGAAAAACAA AGTCCTAATA 
TTTGGACTCC TTGAGGAGAC GATCCTGGCA GCTTGCCTGT CCTACATTCC AGGCATGGAT 
GTAGCCCTGA GAATGTACCC ACTCAAGATA AACTGGTGGT TCTGCGCACT TCCCTACAGT 
GTTCTCATCT TCATCTATGA TGAAGTTCGA AAACTCATCA TCCGCAGACG GCCCGGTGGA 
TGGCTGGAGA AGGAGACGTA CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ouabain interactions with the α4 isoform of the sodium pump trigger non-classical steroid hormone signaling and integrin expression in spermatogenic cells.
Biochimica et biophysica acta1863(11)2809-2819(2016 11)
Upmanyu N,Dietze R,Kirch U,Scheiner-Bobis G

The α4 isoform of the Na⁺,K⁺-ATPase is tuned for changing extracellular environments.
The FEBS journal283(2)282-93(2016 Jan)
Clausen MV,Nissen P,Poulsen H

Green fluorescence protein driven by the Na,K-ATPase α4 isoform promoter is expressed only in male germ cells of mouse testis.
Journal of assisted reproduction and genetics29(12)1313-25(2012 Dec)
McDermott JP,Sánchez G,Chennathukuzhi V,Blanco G

Activity of the Na,K-ATPase α4 isoform is regulated during sperm capacitation to support sperm motility.
Journal of andrology33(5)1047-57(2012 Sep-Oct)
Jimenez T,Sánchez G,Blanco G

Na,K-ATPase alpha4 isoform is essential for sperm fertility.
Proceedings of the National Academy of Sciences of the United States of America108(2)644-9(2011 Jan)
Jimenez T,McDermott JP,Sánchez G,Blanco G

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Atp1a4 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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