Pla2g3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pla2g3
Entrez Gene ID	289733
Full Name	phospholipase A2, group III
General protein information	Preferred Names phospholipase A2, group III Names group 3 secretory phospholipase A2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21

mRNA and Protein(s)

mRNA	Protein	Name
XM_017599118.1	XP_017454607.1	group 3 secretory phospholipase A2 isoform X1
XM_017599119.1	XP_017454608.1	group 3 secretory phospholipase A2 isoform X2
NM_001106015.1	NP_001099485.1	group 3 secretory phospholipase A2 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pla2g3	NP_001099485.1
Bos taurus (cattle)	PLA2G3	XP_005218230.1
Homo sapiens (human)	PLA2G3	NP_056530.2
Mus musculus (house mouse)	Pla2g3	NP_766379.2
Pan troglodytes (chimpanzee)	PLA2G3	XP_525567.2
Macaca mulatta (Rhesus monkey)	PLA2G3	XP_001110828.2
Canis lupus familiaris (dog)	PLA2G3	XP_543487.3
Danio rerio (zebrafish)	zgc:123275	NP_001032489.1

Gene Ontology
Bibliography

Related articles in PubMed

Mast cell maturation is driven via a group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis.
Taketomi Y, Ueno N, Kojima T, Sato H, Murase R, Yamamoto K, Tanaka S, Sakanaka M, Nakamura M, Nishito Y, Kawana M, Kambe N, Ikeda K, Taguchi R, Nakamizo S, Kabashima K, Gelb MH, Arita M, Yokomizo T, Nakamura M, Watanabe K, Hirai H, Nakamura M, Okayama Y, Ra C, Aritake K, Urade Y, Morimoto K, Sugimoto Y, Shimizu T, Narumiya S, Hara S, Murakami M
Nature immunology14(6)554-63(2013 Jun)

Expression and localization of sPLA2-III in the rat CNS.
Yang H, Siddiqi NJ, Alhomida AS, Ong WY
Neurochemical research38(4)753-60(2013 Apr)

Group III secreted phospholipase A2 regulates epididymal sperm maturation and fertility in mice.
Sato H, Taketomi Y, Isogai Y, Miki Y, Yamamoto K, Masuda S, Hosono T, Arata S, Ishikawa Y, Ishii T, Kobayashi T, Nakanishi H, Ikeda K, Taguchi R, Hara S, Kudo I, Murakami M
The Journal of clinical investigation120(5)1400-14(2010 May)

Functional genomic screen for modulators of ciliogenesis and cilium length.
Kim J, Lee JE, Heynen-Genel S, Suyama E, Ono K, Lee K, Ideker T, Aza-Blanc P, Gleeson JG
Nature464(7291)1048-51(2010 Apr)

Group III secreted phospholipase A2 causes apoptosis in rat primary cortical neuronal cultures.
DeCoster MA
Brain research988(1-2)20-8(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pla2g3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pla2g3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa58254	XM_017599118.1 Latest version!	Rattus norvegicus phospholipase A2, group III (Pla2g3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa58255	XM_017599119.1 Latest version!	Rattus norvegicus phospholipase A2, group III (Pla2g3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa11837	NM_001106015.1 Latest version!	Rattus norvegicus phospholipase A2, group III (Pla2g3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pla2g3 ( XM_017599118.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454607.1 Rattus norvegicus phospholipase A2, group III (Pla2g3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58254
Clone ID Related Accession (Same CDS sequence)	XM_017599118.1
Accession Version	XM_017599118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	group 3 secretory phospholipase A2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAGG CCTGGCATTA TTCCAGGCCC TCTGGGATGC GCACCACAGG CTGCAGGTTT 
GTATCAGGCA GGATGAATCC GAGCTCATCG CAGCCTTCAG GGCTCTCTGT GCTCATGAGC 
CCTTGCGGCA CGCCTTCATC CACACCCCTG GACCAGAGCT GCAGAGAGCA CTGGCCACTC 
TGCAGAGCCA GTGGGAGGCC TGCCGAAGGT CTGAGGCTAG TCCCACGGGA GCCAGGGAGA 
AGCGAGAAAC AGAGCACCGC GGAGCACCTG CCGGAGAGCA CCAACGAAGG AGAAGAGGCT 
GGACCATTCC TGGCACGCTG TGGTGCGGTG TTGGGAACTC TGCTGAGAAT GCCTCAGAAC 
TGGGTGAGCC GCAGGGGTTT GGGTATGTTC CACGGCCCTG ATTTCTGCTG CCGGGAGCAC 
GACCAGTGCC CACAAACCAT CTCACCCTTG CAGTATAATT ACGGCATCCG AAACTTCCGA 
TTCCACACCA TCTCTCACTG CGACTGTGAT GCCAGGTTTC AACAGTGCCT GAGGAGCCAG 
GGTGACTCCA TCGCGGACAT CATGGGCGTG GCTTTCTTCA ACGTGCTGGA GATCCCCTGC 
TTTGTGCTGA AGGAGCAGGA GACCTGTGTG GCTTGGCACT GGTGGGGCGG GTGCAGGACA 
TATGGCTCCT TACCCCTTGC TCACCTGCAG CCCAGGACTT ACTACAACGC CTCCTGGAAG 
GCTGAAGCCA CCCCACTTAC TCCCAGCCCC CAAAGCCCAG CACCCAGCTG GAAGAGGGGC 
CCACAGCAGA CACCAGCCAG ACACCACAGC ACAACCACCG TCACTCCCCT CCAGACCCCT 
GCCATCTCCT CCAGACCTGA TATGATGATC CCGAGAGGCC AGCCAGGAGT CTCCCATCCC 
GGCCTCCAGG ATGGCCCAAA ACGTCAAGGT GCCCACCGTG TCTGTCGAAG CCTTCGCCAC 
CTGGATCAGT GCGAGCACCA GATCAAGCCC CAGGAAACCA AGTTCCACCT GCTCAACAGC 
GCCCAGACGC CCCTTTTCCA TTGCAATTGC ACCCGCCGTC TGGCACGTTT CCTGAGGCAC 
CACAGCCTGC CTGCAAACAC CAACAAGGTT TGGGAGCTCC TGGGTACTAC CTGCTTCAAG 
CTGGCCCCAC AACTCAACTG TCCTGAGGGC AAAGGCTGTT CCAGAGACCA TAGGGCCATC 
AAGGTGTCAG CTCGGCATTT GCAGAGGCTT CATCAGAATG GACTCCATTT CTGGGATAAA 
GGCACCGGGG AGGTTCTGGC ACAGCCTTTG GAGCCCCCGG GGACCCTCAT GTCATTCTAC 
AGCCAATGTC TGCAACTAAC CCAGGCAATC TGGAGACCCG GGGGACAGAA GAAATTCTGG 
AGCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454607.1
CDS	311..1699
Translation	MPKAWHYSRPSGMRTTGCRFVSGRMNPSSSQPSGLSVLMSPCGTPSSTPLDQSCREHWPLCRASGRPAEGLRLVPREPGRSEKQSTAEHLPESTNEGEEAGPFLARCGAVLGTLLRMPQNWVSRRGLGMFHGPDFCCREHDQCPQTISPLQYNYGIRNFRFHTISHCDCDARFQQCLRSQGDSIADIMGVAFFNVLEIPCFVLKEQETCVAWHWWGGCRTYGSLPLAHLQPRTYYNASWKAEATPLTPSPQSPAPSWKRGPQQTPARHHSTTTVTPLQTPAISSRPDMMIPRGQPGVSHPGLQDGPKRQGAHRVCRSLRHLDQCEHQIKPQETKFHLLNSAQTPLFHCNCTRRLARFLRHHSLPANTNKVWELLGTTCFKLAPQLNCPEGKGCSRDHRAIKVSARHLQRLHQNGLHFWDKGTGEVLAQPLEPPGTLMSFYSQCLQLTQAIWRPGGQKKFWSS

Target ORF information:

RefSeq Version	XM_017599118.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase A2, group III (Pla2g3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599118.1

ORF Insert Sequence:

ATGCCCAAGG CCTGGCATTA TTCCAGGCCC TCTGGGATGC GCACCACAGG CTGCAGGTTT 
GTATCAGGCA GGATGAATCC GAGCTCATCG CAGCCTTCAG GGCTCTCTGT GCTCATGAGC 
CCTTGCGGCA CGCCTTCATC CACACCCCTG GACCAGAGCT GCAGAGAGCA CTGGCCACTC 
TGCAGAGCCA GTGGGAGGCC TGCCGAAGGT CTGAGGCTAG TCCCACGGGA GCCAGGGAGA 
AGCGAGAAAC AGAGCACCGC GGAGCACCTG CCGGAGAGCA CCAACGAAGG AGAAGAGGCT 
GGACCATTCC TGGCACGCTG TGGTGCGGTG TTGGGAACTC TGCTGAGAAT GCCTCAGAAC 
TGGGTGAGCC GCAGGGGTTT GGGTATGTTC CACGGCCCTG ATTTCTGCTG CCGGGAGCAC 
GACCAGTGCC CACAAACCAT CTCACCCTTG CAGTATAATT ACGGCATCCG AAACTTCCGA 
TTCCACACCA TCTCTCACTG CGACTGTGAT GCCAGGTTTC AACAGTGCCT GAGGAGCCAG 
GGTGACTCCA TCGCGGACAT CATGGGCGTG GCTTTCTTCA ACGTGCTGGA GATCCCCTGC 
TTTGTGCTGA AGGAGCAGGA GACCTGTGTG GCTTGGCACT GGTGGGGCGG GTGCAGGACA 
TATGGCTCCT TACCCCTTGC TCACCTGCAG CCCAGGACTT ACTACAACGC CTCCTGGAAG 
GCTGAAGCCA CCCCACTTAC TCCCAGCCCC CAAAGCCCAG CACCCAGCTG GAAGAGGGGC 
CCACAGCAGA CACCAGCCAG ACACCACAGC ACAACCACCG TCACTCCCCT CCAGACCCCT 
GCCATCTCCT CCAGACCTGA TATGATGATC CCGAGAGGCC AGCCAGGAGT CTCCCATCCC 
GGCCTCCAGG ATGGCCCAAA ACGTCAAGGT GCCCACCGTG TCTGTCGAAG CCTTCGCCAC 
CTGGATCAGT GCGAGCACCA GATCAAGCCC CAGGAAACCA AGTTCCACCT GCTCAACAGC 
GCCCAGACGC CCCTTTTCCA TTGCAATTGC ACCCGCCGTC TGGCACGTTT CCTGAGGCAC 
CACAGCCTGC CTGCAAACAC CAACAAGGTT TGGGAGCTCC TGGGTACTAC CTGCTTCAAG 
CTGGCCCCAC AACTCAACTG TCCTGAGGGC AAAGGCTGTT CCAGAGACCA TAGGGCCATC 
AAGGTGTCAG CTCGGCATTT GCAGAGGCTT CATCAGAATG GACTCCATTT CTGGGATAAA 
GGCACCGGGG AGGTTCTGGC ACAGCCTTTG GAGCCCCCGG GGACCCTCAT GTCATTCTAC 
AGCCAATGTC TGCAACTAAC CCAGGCAATC TGGAGACCCG GGGGACAGAA GAAATTCTGG 
AGCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pla2g3 ( XM_017599119.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454608.1 Rattus norvegicus phospholipase A2, group III (Pla2g3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa58255
Clone ID Related Accession (Same CDS sequence)	XM_017599119.1
Accession Version	XM_017599119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	group 3 secretory phospholipase A2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTCC TGGGGATACT TCTGGGGGTG CTGGCTTTCC TGGGGGTTGT TCCAGAAGGC 
TCCCATACCC TCCATTGGGA CAGTACCTCC TGCCATTTGG TCCGGTCCAT CTCTGGTAGC 
CCTCTGGGCT CCCTGAGCTT CTTGGGCGAA GATGCCCAAG GCCTGGCATT ATTCCAGGCC 
CTCTGGGATG CGCACCACAG GCTGCAGGTT TGTATCAGGC AGGATGAATC CGAGCTCATC 
GCAGCCTTCA GGGCTCTCTG TGCTCATGAG CCCTTGCGGC ACGCCTTCAT CCACACCCCT 
GGACCAGAGC TGCAGAGAGC ACTGGCCACT CTGCAGAGCC AGTGGGAGGC CTGCCGAAGG 
TCTGAGGCTA GTCCCACGGG AGCCAGGGAG AAGCGAGAAA CAGAGCACCG CGGAGCACCT 
GCCGGAGAGC ACCAACGAAG GAGAAGAGGC TGGACCATTC CTGGCACGCT GTGGTGCGGT 
GTTGGGAACT CTGCTGAGAA TGCCTCAGAA CTGGGTATGT TCCACGGCCC TGATTTCTGC 
TGCCGGGAGC ACGACCAGTG CCCACAAACC ATCTCACCCT TGCAGTATAA TTACGGCATC 
CGAAACTTCC GATTCCACAC CATCTCTCAC TGCGACTGTG ATGCCAGGTG CAGGACATAT 
GGCTCCTTAC CCCTTGCTCA CCTGCAGCCC AGGACTTACT ACAACGCCTC CTGGAAGGCT 
GAAGCCACCC CACTTACTCC CAGCCCCCAA AGCCCAGCAC CCAGCTGGAA GAGGGGCCCA 
CAGCAGACAC CAGCCAGACA CCACAGCACA ACCACCGTCA CTCCCCTCCA GACCCCTGCC 
ATCTCCTCCA GACCTGATAT GATGATCCCG AGAGGCCAGC CAGGAGTCTC CCATCCCGGC 
CTCCAGGATG GCCCAAAACG TCAAGGTGCC CACCGTGTCT GTCGAAGCCT TCGCCACCTG 
GATCAGTGCG AGCACCAGAT CAAGCCCCAG GAAACCAAGT TCCACCTGCT CAACAGCGCC 
CAGACGCCCC TTTTCCATTG CAATTGCACC CGCCGTCTGG CACGTTTCCT GAGGCACCAC 
AGCCTGCCTG CAAACACCAA CAAGGTTTGG GAGCTCCTGG GTACTACCTG CTTCAAGCTG 
GCCCCACAAC TCAACTGTCC TGAGGGCAAA GGCTGTTCCA GAGACCATAG GGCCATCAAG 
GTGTCAGCTC GGCATTTGCA GAGGCTTCAT CAGAATGGAC TCCATTTCTG GGATAAAGGC 
ACCGGGGAGG TTCTGGCACA GCCTTTGGAG CCCCCGGGGA CCCTCATGTC ATTCTACAGC 
CAATGTCTGC AACTAACCCA GGCAATCTGG AGACCCGGGG GACAGAAGAA ATTCTGGAGC 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454608.1
CDS	160..1545
Translation	MGVLGILLGVLAFLGVVPEGSHTLHWDSTSCHLVRSISGSPLGSLSFLGEDAQGLALFQALWDAHHRLQVCIRQDESELIAAFRALCAHEPLRHAFIHTPGPELQRALATLQSQWEACRRSEASPTGAREKRETEHRGAPAGEHQRRRRGWTIPGTLWCGVGNSAENASELGMFHGPDFCCREHDQCPQTISPLQYNYGIRNFRFHTISHCDCDARCRTYGSLPLAHLQPRTYYNASWKAEATPLTPSPQSPAPSWKRGPQQTPARHHSTTTVTPLQTPAISSRPDMMIPRGQPGVSHPGLQDGPKRQGAHRVCRSLRHLDQCEHQIKPQETKFHLLNSAQTPLFHCNCTRRLARFLRHHSLPANTNKVWELLGTTCFKLAPQLNCPEGKGCSRDHRAIKVSARHLQRLHQNGLHFWDKGTGEVLAQPLEPPGTLMSFYSQCLQLTQAIWRPGGQKKFWSS

Target ORF information:

RefSeq Version	XM_017599119.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase A2, group III (Pla2g3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599119.1

ORF Insert Sequence:

ATGGGGGTCC TGGGGATACT TCTGGGGGTG CTGGCTTTCC TGGGGGTTGT TCCAGAAGGC 
TCCCATACCC TCCATTGGGA CAGTACCTCC TGCCATTTGG TCCGGTCCAT CTCTGGTAGC 
CCTCTGGGCT CCCTGAGCTT CTTGGGCGAA GATGCCCAAG GCCTGGCATT ATTCCAGGCC 
CTCTGGGATG CGCACCACAG GCTGCAGGTT TGTATCAGGC AGGATGAATC CGAGCTCATC 
GCAGCCTTCA GGGCTCTCTG TGCTCATGAG CCCTTGCGGC ACGCCTTCAT CCACACCCCT 
GGACCAGAGC TGCAGAGAGC ACTGGCCACT CTGCAGAGCC AGTGGGAGGC CTGCCGAAGG 
TCTGAGGCTA GTCCCACGGG AGCCAGGGAG AAGCGAGAAA CAGAGCACCG CGGAGCACCT 
GCCGGAGAGC ACCAACGAAG GAGAAGAGGC TGGACCATTC CTGGCACGCT GTGGTGCGGT 
GTTGGGAACT CTGCTGAGAA TGCCTCAGAA CTGGGTATGT TCCACGGCCC TGATTTCTGC 
TGCCGGGAGC ACGACCAGTG CCCACAAACC ATCTCACCCT TGCAGTATAA TTACGGCATC 
CGAAACTTCC GATTCCACAC CATCTCTCAC TGCGACTGTG ATGCCAGGTG CAGGACATAT 
GGCTCCTTAC CCCTTGCTCA CCTGCAGCCC AGGACTTACT ACAACGCCTC CTGGAAGGCT 
GAAGCCACCC CACTTACTCC CAGCCCCCAA AGCCCAGCAC CCAGCTGGAA GAGGGGCCCA 
CAGCAGACAC CAGCCAGACA CCACAGCACA ACCACCGTCA CTCCCCTCCA GACCCCTGCC 
ATCTCCTCCA GACCTGATAT GATGATCCCG AGAGGCCAGC CAGGAGTCTC CCATCCCGGC 
CTCCAGGATG GCCCAAAACG TCAAGGTGCC CACCGTGTCT GTCGAAGCCT TCGCCACCTG 
GATCAGTGCG AGCACCAGAT CAAGCCCCAG GAAACCAAGT TCCACCTGCT CAACAGCGCC 
CAGACGCCCC TTTTCCATTG CAATTGCACC CGCCGTCTGG CACGTTTCCT GAGGCACCAC 
AGCCTGCCTG CAAACACCAA CAAGGTTTGG GAGCTCCTGG GTACTACCTG CTTCAAGCTG 
GCCCCACAAC TCAACTGTCC TGAGGGCAAA GGCTGTTCCA GAGACCATAG GGCCATCAAG 
GTGTCAGCTC GGCATTTGCA GAGGCTTCAT CAGAATGGAC TCCATTTCTG GGATAAAGGC 
ACCGGGGAGG TTCTGGCACA GCCTTTGGAG CCCCCGGGGA CCCTCATGTC ATTCTACAGC 
CAATGTCTGC AACTAACCCA GGCAATCTGG AGACCCGGGG GACAGAAGAA ATTCTGGAGC 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pla2g3 ( NM_001106015.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099485.1 Rattus norvegicus phospholipase A2, group III (Pla2g3), mRNA.

Price & Availability↑

CloneID	ORa11837
Clone ID Related Accession (Same CDS sequence)	NM_001106015.1
Accession Version	NM_001106015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Rattus norvegicus(Norway rat)
Product	group 3 secretory phospholipase A2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473963.1. On or before Oct 4, 2007 this sequence version replaced XM_223553.4, XM_001066611.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689602, SAMN04563757 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGTCC TGGGGATACT TCTGGGGGTG CTGGCTTTCC TGGGGGTTGT TCCAGAAGGC 
TCCCATACCC TCCATTGGGA CAGTACCTCC TGCCATTTGG TCCGGTCCAT CTCTGGTAGC 
CCTCTGGGCT CCCTGAGCTT CTTGGGCGAA GATGCCCAAG GCCTGGCATT ATTCCAGGCC 
CTCTGGGATG CGCACCACAG GCTGCAGGTT TGTATCAGGC AGGATGAATC CGAGCTCATC 
GCAGCCTTCA GGGCTCTCTG TGCTCATGAG CCCTTGCGGC ACGCCTTCAT CCACACCCCT 
GGACCAGAGC TGCAGAGAGC ACTGGCCACT CTGCAGAGCC AGTGGGAGGC CTGCCGAAGG 
TCTGAGGCTA GTCCCACGGG AGCCAGGGAG AAGCGAGAAA CAGAGCACCG CGGAGCACCT 
GCCGGAGAGC ACCAACGAAG GAGAAGAGGC TGGACCATTC CTGGCACGCT GTGGTGCGGT 
GTTGGGAACT CTGCTGAGAA TGCCTCAGAA CTGGGTATGT TCCACGGCCC TGATTTCTGC 
TGCCGGGAGC ACGACCAGTG CCCACAAACC ATCTCACCCT TGCAGTATAA TTACGGCATC 
CGAAACTTCC GATTCCACAC CATCTCTCAC TGCGACTGTG ATGCCAGGTT TCAACAGTGC 
CTGAGGAGCC AGGGTGACTC CATCGCGGAC ATCATGGGCG TGGCTTTCTT CAACGTGCTG 
GAGATCCCCT GCTTTGTGCT GAAGGAGCAG GAGACCTGTG TGGCTTGGCA CTGGTGGGGC 
GGGTGCAGGA CATATGGCTC CTTACCCCTT GCTCACCTGC AGCCCAGGAC TTACTACAAC 
GCCTCCTGGA AGGCTGAAGC CACCCCACTT ACTCCCAGCC CCCAAAGCCC AGCACCCAGC 
TGGAAGAGGG GCCCACAGCA GACACCAGCC AGACACCACA GCACAACCAC CGTCACTCCC 
CTCCAGACCC CTGCCATCTC CTCCAGACCT GATATGATGA TCCCGAGAGG CCAGCCAGGA 
GTCTCCCATC CCGGCCTCCA GGATGGCCCA AAACGTCAAG GTGCCCACCG TGTCTGTCGA 
AGCCTTCGCC ACCTGGATCA GTGCGAGCAC CAGATCAAGC CCCAGGAAAC CAAGTTCCAC 
CTGCTCAACA GCGCCCAGAC GCCCCTTTTC CATTGCAATT GCACCCGCCG TCTGGCACGT 
TTCCTGAGGC ACCACAGCCT GCCTGCAAAC ACCAACAAGG TTTGGGAGCT CCTGGGTACT 
ACCTGCTTCA AGCTGGCCCC ACAACTCAAC TGTCCTGAGG GCAAAGGCTG TTCCAGAGAC 
CATAGGGCCA TCAAGGTGTC AGCTCGGCAT TTGCAGAGGC TTCATCAGAA TGGACTCCAT 
TTCTGGGATA AAGGCACCGG GGAGGTTCTG GCACAGCCTT TGGAGCCCCC GGGGACCCTC 
ATGTCATTCT ACAGCCAATG TCTGCAACTA ACCCAGGCAA TCTGGAGACC CGGGGGACAG 
AAGAAATTCT GGAGCTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099485.1
CDS	55..1575
Translation	MGVLGILLGVLAFLGVVPEGSHTLHWDSTSCHLVRSISGSPLGSLSFLGEDAQGLALFQALWDAHHRLQVCIRQDESELIAAFRALCAHEPLRHAFIHTPGPELQRALATLQSQWEACRRSEASPTGAREKRETEHRGAPAGEHQRRRRGWTIPGTLWCGVGNSAENASELGMFHGPDFCCREHDQCPQTISPLQYNYGIRNFRFHTISHCDCDARFQQCLRSQGDSIADIMGVAFFNVLEIPCFVLKEQETCVAWHWWGGCRTYGSLPLAHLQPRTYYNASWKAEATPLTPSPQSPAPSWKRGPQQTPARHHSTTTVTPLQTPAISSRPDMMIPRGQPGVSHPGLQDGPKRQGAHRVCRSLRHLDQCEHQIKPQETKFHLLNSAQTPLFHCNCTRRLARFLRHHSLPANTNKVWELLGTTCFKLAPQLNCPEGKGCSRDHRAIKVSARHLQRLHQNGLHFWDKGTGEVLAQPLEPPGTLMSFYSQCLQLTQAIWRPGGQKKFWSS

Target ORF information:

RefSeq Version	NM_001106015.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase A2, group III (Pla2g3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106015.1

ORF Insert Sequence:

ATGGGGGTCC TGGGGATACT TCTGGGGGTG CTGGCTTTCC TGGGGGTTGT TCCAGAAGGC 
TCCCATACCC TCCATTGGGA CAGTACCTCC TGCCATTTGG TCCGGTCCAT CTCTGGTAGC 
CCTCTGGGCT CCCTGAGCTT CTTGGGCGAA GATGCCCAAG GCCTGGCATT ATTCCAGGCC 
CTCTGGGATG CGCACCACAG GCTGCAGGTT TGTATCAGGC AGGATGAATC CGAGCTCATC 
GCAGCCTTCA GGGCTCTCTG TGCTCATGAG CCCTTGCGGC ACGCCTTCAT CCACACCCCT 
GGACCAGAGC TGCAGAGAGC ACTGGCCACT CTGCAGAGCC AGTGGGAGGC CTGCCGAAGG 
TCTGAGGCTA GTCCCACGGG AGCCAGGGAG AAGCGAGAAA CAGAGCACCG CGGAGCACCT 
GCCGGAGAGC ACCAACGAAG GAGAAGAGGC TGGACCATTC CTGGCACGCT GTGGTGCGGT 
GTTGGGAACT CTGCTGAGAA TGCCTCAGAA CTGGGTATGT TCCACGGCCC TGATTTCTGC 
TGCCGGGAGC ACGACCAGTG CCCACAAACC ATCTCACCCT TGCAGTATAA TTACGGCATC 
CGAAACTTCC GATTCCACAC CATCTCTCAC TGCGACTGTG ATGCCAGGTT TCAACAGTGC 
CTGAGGAGCC AGGGTGACTC CATCGCGGAC ATCATGGGCG TGGCTTTCTT CAACGTGCTG 
GAGATCCCCT GCTTTGTGCT GAAGGAGCAG GAGACCTGTG TGGCTTGGCA CTGGTGGGGC 
GGGTGCAGGA CATATGGCTC CTTACCCCTT GCTCACCTGC AGCCCAGGAC TTACTACAAC 
GCCTCCTGGA AGGCTGAAGC CACCCCACTT ACTCCCAGCC CCCAAAGCCC AGCACCCAGC 
TGGAAGAGGG GCCCACAGCA GACACCAGCC AGACACCACA GCACAACCAC CGTCACTCCC 
CTCCAGACCC CTGCCATCTC CTCCAGACCT GATATGATGA TCCCGAGAGG CCAGCCAGGA 
GTCTCCCATC CCGGCCTCCA GGATGGCCCA AAACGTCAAG GTGCCCACCG TGTCTGTCGA 
AGCCTTCGCC ACCTGGATCA GTGCGAGCAC CAGATCAAGC CCCAGGAAAC CAAGTTCCAC 
CTGCTCAACA GCGCCCAGAC GCCCCTTTTC CATTGCAATT GCACCCGCCG TCTGGCACGT 
TTCCTGAGGC ACCACAGCCT GCCTGCAAAC ACCAACAAGG TTTGGGAGCT CCTGGGTACT 
ACCTGCTTCA AGCTGGCCCC ACAACTCAAC TGTCCTGAGG GCAAAGGCTG TTCCAGAGAC 
CATAGGGCCA TCAAGGTGTC AGCTCGGCAT TTGCAGAGGC TTCATCAGAA TGGACTCCAT 
TTCTGGGATA AAGGCACCGG GGAGGTTCTG GCACAGCCTT TGGAGCCCCC GGGGACCCTC 
ATGTCATTCT ACAGCCAATG TCTGCAACTA ACCCAGGCAA TCTGGAGACC CGGGGGACAG 
AAGAAATTCT GGAGCTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mast cell maturation is driven via a group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis.
Nature immunology14(6)554-63(2013 Jun)
Taketomi Y,Ueno N,Kojima T,Sato H,Murase R,Yamamoto K,Tanaka S,Sakanaka M,Nakamura M,Nishito Y,Kawana M,Kambe N,Ikeda K,Taguchi R,Nakamizo S,Kabashima K,Gelb MH,Arita M,Yokomizo T,Nakamura M,Watanabe K,Hirai H,Nakamura M,Okayama Y,Ra C,Aritake K,Urade Y,Morimoto K,Sugimoto Y,Shimizu T,Narumiya S,Hara S,Murakami M

Expression and localization of sPLA2-III in the rat CNS.
Neurochemical research38(4)753-60(2013 Apr)
Yang H,Siddiqi NJ,Alhomida AS,Ong WY

Group III secreted phospholipase A2 regulates epididymal sperm maturation and fertility in mice.
The Journal of clinical investigation120(5)1400-14(2010 May)
Sato H,Taketomi Y,Isogai Y,Miki Y,Yamamoto K,Masuda S,Hosono T,Arata S,Ishikawa Y,Ishii T,Kobayashi T,Nakanishi H,Ikeda K,Taguchi R,Hara S,Kudo I,Murakami M

Functional genomic screen for modulators of ciliogenesis and cilium length.
Nature464(7291)1048-51(2010 Apr)
Kim J,Lee JE,Heynen-Genel S,Suyama E,Ono K,Lee K,Ideker T,Aza-Blanc P,Gleeson JG

Group III secreted phospholipase A2 causes apoptosis in rat primary cortical neuronal cultures.
Brain research988(1-2)20-8(2003 Oct)
DeCoster MA

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Pla2g3 cDNA ORF clone, Rattus norvegicus(Norway rat)

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