Smyd2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smyd2
Entrez Gene ID	289372
Full Name	SET and MYND domain containing 2
Synonyms	HSKM-B
General protein information	Preferred Names SET and MYND domain containing 2 Names N-lysine methyltransferase SMYD2 SET and MYND domain-containing protein 2 histone methyltransferase SMYD2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q27

mRNA and Protein(s)

mRNA	Protein	Name
NM_206851.1	NP_996733.1	N-lysine methyltransferase SMYD2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMYD2	XP_537149.3
Xenopus tropicalis (tropical clawed frog)	LOC100495522	XP_002934751.2
Homo sapiens (human)	SMYD2	NP_064582.2
Rattus norvegicus (Norway rat)	Smyd2	NP_996733.1
Danio rerio (zebrafish)	smyd2a	NP_001013568.1
Pan troglodytes (chimpanzee)	SMYD2	XP_003308794.1
Macaca mulatta (Rhesus monkey)	SMYD2	XP_001106482.2
Bos taurus (cattle)	SMYD2	NP_001069832.1
Mus musculus (house mouse)	Smyd2	NP_081072.1
Gallus gallus (chicken)	SMYD2	NP_001264500.1

Gene Ontology
Bibliography

Related articles in PubMed

SMYD2 glutathionylation contributes to degradation of sarcomeric proteins.
Munkanatta Godage DNP, VanHecke GC, Samarasinghe KTG, Feng HZ, Hiske M, Holcomb J, Yang Z, Jin JP, Chung CS, Ahn YH
Nature communications9(1)4341(2018 10)

SMYD2-dependent HSP90 methylation promotes cancer cell proliferation by regulating the chaperone complex formation.
Hamamoto R, Toyokawa G, Nakakido M, Ueda K, Nakamura Y
Cancer letters351(1)126-33(2014 Aug)

Lysine methyltransferase Smyd2 regulates Hsp90-mediated protection of the sarcomeric titin springs and cardiac function.
Voelkel T, Andresen C, Unger A, Just S, Rottbauer W, Linke WA
Biochimica et biophysica acta1833(4)812-22(2013 Apr)

Histone lysine methylation dynamics: establishment, regulation, and biological impact.
Black JC, Van Rechem C, Whetstine JR
Molecular cell48(4)491-507(2012 Nov)

Pax3/7BP is a Pax7- and Pax3-binding protein that regulates the proliferation of muscle precursor cells by an epigenetic mechanism.
Diao Y, Guo X, Li Y, Sun K, Lu L, Jiang L, Fu X, Zhu H, Sun H, Wang H, Wu Z
Cell stem cell11(2)231-41(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Smyd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smyd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04524	NM_206851.1 Latest version!	Rattus norvegicus SET and MYND domain containing 2 (Smyd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smyd2 ( NM_206851.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_996733.1 Rattus norvegicus SET and MYND domain containing 2 (Smyd2), mRNA.

Price & Availability↑

CloneID	ORa04524
Clone ID Related Accession (Same CDS sequence)	NM_206851.1
Accession Version	NM_206851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550678400000
Organism	Rattus norvegicus(Norway rat)
Product	N-lysine methyltransferase SMYD2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB726546.1, BK001057.1 and AW433707.1. On or before Dec 17, 2009 this sequence version replaced XM_001066311.1, XM_213972.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BK001057.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCGCCG AGGCCCGCGG CGGCCTGGAG CGCTTCTGCA GCGCGGGCAA GGGCCGGGGG 
CTCCGTGCGC TGCGGCCCTT CCACGTGGGC GACCTCCTCT TCTCCTGCCC GGCCTACGCC 
TGCGTGCTCA CCGTGGGCGA GCGGGGCCAC CACTGCGAGT GCTGCTTCGC CAGGAAAGAA 
GGATTGTCGA AATGTGGACG ATGCAAGCAA GCCTTCTACT GCGATGTGGA GTGTCAGAAA 
GAAGACTGGC CCCTGCACAA GCTGGAGTGC TCCTCCATGG TTGTTTTCGG GGAGAACTGG 
AATCCCTCGG AGACTGTGCG GCTCACAGCA AGGATCCTGG CCAAGCAGAA AATGCACCCA 
GAGAGGACAC CTTCAGAGAA ACTGTTGGCC GTGAGGGAGT TTGAGTCACA TCTGGACAAG 
CTAGACAACG AGAAGAAGGA TCTCATCCAG AGCGACATCG CGGCGCTCCA TCAGTTCTAC 
TCCAAGCACC TGGAGTTCCC TGACCACAGC AGCCTTGTGG TGCTCTTTGC CCAGGTGAAC 
TGTAATGGCT TCACTATTGA AGATGAGGAG CTCTCTCACT TGGGATCGGC GATATTTCCT 
GATGTTGCGC TGATGAATCA CAGCTGCTGC CCGAATGTCA TTGTGACCTA CAAAGGTACC 
CTGGCAGAAG TCAGAGCTGT GCAGGAGATC CACCCAGGAG ATGAGGTGTT CACCAGCTAT 
ATCGACCTGC TGTATCCAAC AGAAGACAGG AACGACCGGT TAAGAGACTC CTACTTCTTT 
ACCTGTGAGT GCCGGGAGTG TACGACCAAG GACAAGGACA AGGCCAAGGT GGAAATCCGG 
AAGCTCAGCA ACCCACCTCA GGCAGAAGCC ATCCGAGACA TGGTCAGATA CGCACGCAAT 
GTCATCGAGG AGTTCCGGAG GGCCAAGCAC TACAAATCCC CTAGTGAGCT GTTGGAAATC 
TGTGAGCTCA GCCAGGAGAA GATGAGCTCT GTGTTTGAGG ACAGCAATGT GTACATGCTA 
CACATGATGT ACCAGGCCAT GGGCGTCTGC CTGTACATGC AGGACTGGGA AGGAGCCCTG 
AAATATGGCC AGAAGATCAT CAAACCCTAC AGTAAGCACT ACCCCGTGTA CTCCCTCAAC 
GTGGCCTCCA TGTGGCTGAA GTTGGGAAGA CTGTACATGG GCCTGGAGAA CAAAGCTGCC 
GGGGAGAAGG CCCTGAAGAA GGCCATCGCC ATCATGGAAA TAGCTCATGG CAAGGACCAC 
CCGTACATCT CCGAGATCAA GCAGGAAATT GAGAGCCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996733.1
CDS	35..1336
Translation	MRAEARGGLERFCSAGKGRGLRALRPFHVGDLLFSCPAYACVLTVGERGHHCECCFARKEGLSKCGRCKQAFYCDVECQKEDWPLHKLECSSMVVFGENWNPSETVRLTARILAKQKMHPERTPSEKLLAVREFESHLDKLDNEKKDLIQSDIAALHQFYSKHLEFPDHSSLVVLFAQVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNVIVTYKGTLAEVRAVQEIHPGDEVFTSYIDLLYPTEDRNDRLRDSYFFTCECRECTTKDKDKAKVEIRKLSNPPQAEAIRDMVRYARNVIEEFRRAKHYKSPSELLEICELSQEKMSSVFEDSNVYMLHMMYQAMGVCLYMQDWEGALKYGQKIIKPYSKHYPVYSLNVASMWLKLGRLYMGLENKAAGEKALKKAIAIMEIAHGKDHPYISEIKQEIESH

Target ORF information:

RefSeq Version	NM_206851.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SET and MYND domain containing 2 (Smyd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206851.1

ORF Insert Sequence:

ATGCGCGCCG AGGCCCGCGG CGGCCTGGAG CGCTTCTGCA GCGCGGGCAA GGGCCGGGGG 
CTCCGTGCGC TGCGGCCCTT CCACGTGGGC GACCTCCTCT TCTCCTGCCC GGCCTACGCC 
TGCGTGCTCA CCGTGGGCGA GCGGGGCCAC CACTGCGAGT GCTGCTTCGC CAGGAAAGAA 
GGATTGTCGA AATGTGGACG ATGCAAGCAA GCCTTCTACT GCGATGTGGA GTGTCAGAAA 
GAAGACTGGC CCCTGCACAA GCTGGAGTGC TCCTCCATGG TTGTTTTCGG GGAGAACTGG 
AATCCCTCGG AGACTGTGCG GCTCACAGCA AGGATCCTGG CCAAGCAGAA AATGCACCCA 
GAGAGGACAC CTTCAGAGAA ACTGTTGGCC GTGAGGGAGT TTGAGTCACA TCTGGACAAG 
CTAGACAACG AGAAGAAGGA TCTCATCCAG AGCGACATCG CGGCGCTCCA TCAGTTCTAC 
TCCAAGCACC TGGAGTTCCC TGACCACAGC AGCCTTGTGG TGCTCTTTGC CCAGGTGAAC 
TGTAATGGCT TCACTATTGA AGATGAGGAG CTCTCTCACT TGGGATCGGC GATATTTCCT 
GATGTTGCGC TGATGAATCA CAGCTGCTGC CCGAATGTCA TTGTGACCTA CAAAGGTACC 
CTGGCAGAAG TCAGAGCTGT GCAGGAGATC CACCCAGGAG ATGAGGTGTT CACCAGCTAT 
ATCGACCTGC TGTATCCAAC AGAAGACAGG AACGACCGGT TAAGAGACTC CTACTTCTTT 
ACCTGTGAGT GCCGGGAGTG TACGACCAAG GACAAGGACA AGGCCAAGGT GGAAATCCGG 
AAGCTCAGCA ACCCACCTCA GGCAGAAGCC ATCCGAGACA TGGTCAGATA CGCACGCAAT 
GTCATCGAGG AGTTCCGGAG GGCCAAGCAC TACAAATCCC CTAGTGAGCT GTTGGAAATC 
TGTGAGCTCA GCCAGGAGAA GATGAGCTCT GTGTTTGAGG ACAGCAATGT GTACATGCTA 
CACATGATGT ACCAGGCCAT GGGCGTCTGC CTGTACATGC AGGACTGGGA AGGAGCCCTG 
AAATATGGCC AGAAGATCAT CAAACCCTAC AGTAAGCACT ACCCCGTGTA CTCCCTCAAC 
GTGGCCTCCA TGTGGCTGAA GTTGGGAAGA CTGTACATGG GCCTGGAGAA CAAAGCTGCC 
GGGGAGAAGG CCCTGAAGAA GGCCATCGCC ATCATGGAAA TAGCTCATGG CAAGGACCAC 
CCGTACATCT CCGAGATCAA GCAGGAAATT GAGAGCCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SMYD2 glutathionylation contributes to degradation of sarcomeric proteins.
Nature communications9(1)4341(2018 10)
Munkanatta Godage DNP,VanHecke GC,Samarasinghe KTG,Feng HZ,Hiske M,Holcomb J,Yang Z,Jin JP,Chung CS,Ahn YH

SMYD2-dependent HSP90 methylation promotes cancer cell proliferation by regulating the chaperone complex formation.
Cancer letters351(1)126-33(2014 Aug)
Hamamoto R,Toyokawa G,Nakakido M,Ueda K,Nakamura Y

Lysine methyltransferase Smyd2 regulates Hsp90-mediated protection of the sarcomeric titin springs and cardiac function.
Biochimica et biophysica acta1833(4)812-22(2013 Apr)
Voelkel T,Andresen C,Unger A,Just S,Rottbauer W,Linke WA

Histone lysine methylation dynamics: establishment, regulation, and biological impact.
Molecular cell48(4)491-507(2012 Nov)
Black JC,Van Rechem C,Whetstine JR

Pax3/7BP is a Pax7- and Pax3-binding protein that regulates the proliferation of muscle precursor cells by an epigenetic mechanism.
Cell stem cell11(2)231-41(2012 Aug)
Diao Y,Guo X,Li Y,Sun K,Lu L,Jiang L,Fu X,Zhu H,Sun H,Wang H,Wu Z