Pgam5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pgam5
Entrez Gene ID	288731
Full Name	PGAM family member 5, mitochondrial serine/threonine protein phosphatase
Synonyms	RGD1312028
General protein information	Preferred Names PGAM family member 5, mitochondrial serine/threonine protein phosphatase Names serine/threonine-protein phosphatase PGAM5, mitochondrial phosphoglycerate mutase family member 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16

mRNA and Protein(s)

mRNA	Protein	Name
NM_001025272.1	NP_001020443.1	serine/threonine-protein phosphatase PGAM5, mitochondrial
XM_006249534.3	XP_006249596.1	serine/threonine-protein phosphatase PGAM5, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PGAM5	NP_001179120.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002535	XP_312403.4
Homo sapiens (human)	PGAM5	NP_001164015.1
Mus musculus (house mouse)	Pgam5	NP_082549.2
Drosophila melanogaster (fruit fly)	Pgam5	NP_569951.1
Caenorhabditis elegans (roundworm)	pgam-5	NP_495593.2
Xenopus tropicalis (tropical clawed frog)	pgam5	NP_001004858.1
Pan troglodytes (chimpanzee)	PGAM5	XP_509498.3
Rattus norvegicus (Norway rat)	Pgam5	NP_001020443.1
Gallus gallus (chicken)	PGAM5	XP_415083.4
Canis lupus familiaris (dog)	PGAM5	XP_005636128.1
Danio rerio (zebrafish)	pgam5	NP_001007324.2

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondrial phosphatase PGAM5 regulates Keap1-mediated Bcl-xL degradation and controls cardiomyocyte apoptosis driven by myocardial ischemia/reperfusion injury.
Yang C, Liu X, Yang F, Zhang W, Chen Z, Yan D, You Q, Wu X
In vitro cellular & developmental biology. Animal53(3)248-257(2017 Mar)

The Ablation of Mitochondrial Protein Phosphatase Pgam5 Confers Resistance Against Metabolic Stress.
Sekine S, Yao A, Hattori K, Sugawara S, Naguro I, Koike M, Uchiyama Y, Takeda K, Ichijo H
EBioMedicine582-92(2016 Mar)

Selective removal of mitochondria via mitophagy: distinct pathways for different mitochondrial stresses.
Wei H, Liu L, Chen Q
Biochimica et biophysica acta1853(10 Pt B)2784-90(2015 Oct)

The Nrf2 regulatory network provides an interface between redox and intermediary metabolism.
Hayes JD, Dinkova-Kostova AT
Trends in biochemical sciences39(4)199-218(2014 Apr)

The mitochondrial phosphatase PGAM5 functions at the convergence point of multiple necrotic death pathways.
Wang Z, Jiang H, Chen S, Du F, Wang X
Cell148(1-2)228-43(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pgam5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pgam5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12941	NM_001025272.1 Latest version!	Rattus norvegicus PGAM family member 5, mitochondrial serine/threonine protein phosphatase (Pgam5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa35153	XM_006249534.3 Latest version!	Rattus norvegicus phosphoglycerate mutase family member 5 (Pgam5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pgam5 ( NM_001025272.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001020443.1 Rattus norvegicus PGAM family member 5, mitochondrial serine/threonine protein phosphatase (Pgam5), mRNA.

Price & Availability↑

CloneID	ORa12941
Clone ID Related Accession (Same CDS sequence)	NM_001025272.1
Accession Version	NM_001025272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550246400000
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein phosphatase PGAM5, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC092607.1. On Jul 13, 2005 this sequence version replaced XM_222256.3. ##Evidence-Data-START## Transcript exon combination :: BC092607.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCTTTCC GGCAGGCTCT TCAGCTGGCG GCCTGTGGTC TGGCGGGTGG CTCGGCCGCT 
GTTCTCTTCT CGGCGGTAGC GGTAGGGAAG CCGAGAGCTG GCGGGGATGC AGACACGCGC 
ACGACAGAGC CGCTGGCCTG GACCGGAGCG CGCCCCGGGC ACGGCGTCTG GGACTCCAAC 
TGGGACAGGC GAGAGCCACT GTCACTCATT AACCTGAAGA AAAGGAACGT GGAATTTGGA 
GAAGATGAAC TGGCTTCCAG GCTGGACCAC TATAAGGCAA AGGCTACAAG ACACATATTC 
CTCATCCGGC ATTCCCAGTA CAATGTGGAT GGCTCGATGG AGAAGGACCG CACCCTGACC 
CCATTAGGTC GGGAACAGGC TGAACTCACG GGGATCCGAC TTGCAAGCCT GGGACTGAAG 
TTTAATAAAA TCGTCCATTC CTCTATGACC CGTGCAGTAG AGACCACAGA CATCATCAGT 
AAGCACCTGC CAGGTGTCTG CAGAGTCAGC ACAGACTTGC TACGGGAAGG TGCCCCCATT 
GAACCGGATC CACCTGTGTC TCACTGGAAG CCAGAGGCTG TGCAGTATTA TGAAGATGGA 
GCCCGGATTG AGGCTGCCTT CAGGAACTAC ATCCACCGAG CTGATGCCAA GCAGGAGGAG 
GACAGCTACG AGATCTTCAT ATGTCACGCC AATGTCATCC GTTATATTGT TTGTAGGGCA 
CTGCAGTTTC CCCCAGAAGG TTGGCTCCGC CTGTCCCTCA ACAATGGTAG TATCACCCAC 
CTGGTGATTC GACCCAATGG TCGTGTTGCA CTCAGGACCC TTGGGGACAC AGGGTTCATG 
CCCCCAGACA AGATTACTCG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020443.1
CDS	34..900
Translation	MAFRQALQLAACGLAGGSAAVLFSAVAVGKPRAGGDADTRTTEPLAWTGARPGHGVWDSNWDRREPLSLINLKKRNVEFGEDELASRLDHYKAKATRHIFLIRHSQYNVDGSMEKDRTLTPLGREQAELTGIRLASLGLKFNKIVHSSMTRAVETTDIISKHLPGVCRVSTDLLREGAPIEPDPPVSHWKPEAVQYYEDGARIEAAFRNYIHRADAKQEEDSYEIFICHANVIRYIVCRALQFPPEGWLRLSLNNGSITHLVIRPNGRVALRTLGDTGFMPPDKITRS

Target ORF information:

RefSeq Version	NM_001025272.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PGAM family member 5, mitochondrial serine/threonine protein phosphatase (Pgam5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025272.1

ORF Insert Sequence:

ATGGCTTTCC GGCAGGCTCT TCAGCTGGCG GCCTGTGGTC TGGCGGGTGG CTCGGCCGCT 
GTTCTCTTCT CGGCGGTAGC GGTAGGGAAG CCGAGAGCTG GCGGGGATGC AGACACGCGC 
ACGACAGAGC CGCTGGCCTG GACCGGAGCG CGCCCCGGGC ACGGCGTCTG GGACTCCAAC 
TGGGACAGGC GAGAGCCACT GTCACTCATT AACCTGAAGA AAAGGAACGT GGAATTTGGA 
GAAGATGAAC TGGCTTCCAG GCTGGACCAC TATAAGGCAA AGGCTACAAG ACACATATTC 
CTCATCCGGC ATTCCCAGTA CAATGTGGAT GGCTCGATGG AGAAGGACCG CACCCTGACC 
CCATTAGGTC GGGAACAGGC TGAACTCACG GGGATCCGAC TTGCAAGCCT GGGACTGAAG 
TTTAATAAAA TCGTCCATTC CTCTATGACC CGTGCAGTAG AGACCACAGA CATCATCAGT 
AAGCACCTGC CAGGTGTCTG CAGAGTCAGC ACAGACTTGC TACGGGAAGG TGCCCCCATT 
GAACCGGATC CACCTGTGTC TCACTGGAAG CCAGAGGCTG TGCAGTATTA TGAAGATGGA 
GCCCGGATTG AGGCTGCCTT CAGGAACTAC ATCCACCGAG CTGATGCCAA GCAGGAGGAG 
GACAGCTACG AGATCTTCAT ATGTCACGCC AATGTCATCC GTTATATTGT TTGTAGGGCA 
CTGCAGTTTC CCCCAGAAGG TTGGCTCCGC CTGTCCCTCA ACAATGGTAG TATCACCCAC 
CTGGTGATTC GACCCAATGG TCGTGTTGCA CTCAGGACCC TTGGGGACAC AGGGTTCATG 
CCCCCAGACA AGATTACTCG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pgam5 ( XM_006249534.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249596.1 Rattus norvegicus phosphoglycerate mutase family member 5 (Pgam5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35153
Clone ID Related Accession (Same CDS sequence)	XM_006249534.3
Accession Version	XM_006249534.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein phosphatase PGAM5, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249534.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTCC GGCAGGCTCT TCAGCTGGCG GCCTGTGGTC TGGCGGGTGG CTCGGCCGCT 
GTTCTCTTCT CGGCGGTAGC GGTAGGGAAG CCGAGAGCTG GCGGGGATGC AGACACGCGC 
ACGACAGAGC CGCTGGCCTG GACCGGAGCG CGCCCCGGGC ACGGCGTCTG GGACTCCAAC 
TGGGACAGGC GAGAGCCACT GTCACTCATT AACCTGAAGA AAAGGAACGT GGAATCTGGA 
GAAGATGAAC TGGCTTCCAG GCTGGACCAC TATAAGGCAA AGGCTACAAG ACACATATTC 
CTCATCCGGC ATTCCCAGTA CAATGTGGAT GGCTCGATGG AGAAGGACCG CACCCTGACC 
CCATTAGGTC GGGAACAGGC TGAACTCACG GGGATCCGAC TTGCAAGCCT GGGACTGAAG 
TTTAATAAAA TCGTCCATTC CTCTATGACC CGTGCAGTAG AGACCACAGA CATCATCAGT 
AAGCACCTGC CAGGTGTCTG CAGAGTCAGC ACAGACTTGC TACGGGAAGG TGCCCCCATT 
GAACCGGATC CACCTGTGTC TCACTGGAAG CCAGAGGCTG TGTATTATGA AGATGGAGCC 
CGGATTGAGG CTGCCTTCAG GAACTACATC CACCGAGCTG ATGCCAAGCA GGAGGAGGAC 
AGCTACGAGA TCTTCATATG TCACGCCAAT GTCATCCGTT ATATTGTTTG TAGGGCACTG 
CAGTTTCCCC CAGAAGGTTG GCTCCGCCTG TCCCTCAACA ATGGTAGTAT CACCCACCTG 
GTGATTCGAC CCAATGGTCG TGTTGCACTC AGGACCCTTG GGGACACAGG GTTCATGCCC 
CCAGACAAGA TTACTCGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249596.1
CDS	67..930
Translation	MAFRQALQLAACGLAGGSAAVLFSAVAVGKPRAGGDADTRTTEPLAWTGARPGHGVWDSNWDRREPLSLINLKKRNVESGEDELASRLDHYKAKATRHIFLIRHSQYNVDGSMEKDRTLTPLGREQAELTGIRLASLGLKFNKIVHSSMTRAVETTDIISKHLPGVCRVSTDLLREGAPIEPDPPVSHWKPEAVYYEDGARIEAAFRNYIHRADAKQEEDSYEIFICHANVIRYIVCRALQFPPEGWLRLSLNNGSITHLVIRPNGRVALRTLGDTGFMPPDKITRS

Target ORF information:

RefSeq Version	XM_006249534.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoglycerate mutase family member 5 (Pgam5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249534.3

ORF Insert Sequence:

ATGGCTTTCC GGCAGGCTCT TCAGCTGGCG GCCTGTGGTC TGGCGGGTGG CTCGGCCGCT 
GTTCTCTTCT CGGCGGTAGC GGTAGGGAAG CCGAGAGCTG GCGGGGATGC AGACACGCGC 
ACGACAGAGC CGCTGGCCTG GACCGGAGCG CGCCCCGGGC ACGGCGTCTG GGACTCCAAC 
TGGGACAGGC GAGAGCCACT GTCACTCATT AACCTGAAGA AAAGGAACGT GGAATCTGGA 
GAAGATGAAC TGGCTTCCAG GCTGGACCAC TATAAGGCAA AGGCTACAAG ACACATATTC 
CTCATCCGGC ATTCCCAGTA CAATGTGGAT GGCTCGATGG AGAAGGACCG CACCCTGACC 
CCATTAGGTC GGGAACAGGC TGAACTCACG GGGATCCGAC TTGCAAGCCT GGGACTGAAG 
TTTAATAAAA TCGTCCATTC CTCTATGACC CGTGCAGTAG AGACCACAGA CATCATCAGT 
AAGCACCTGC CAGGTGTCTG CAGAGTCAGC ACAGACTTGC TACGGGAAGG TGCCCCCATT 
GAACCGGATC CACCTGTGTC TCACTGGAAG CCAGAGGCTG TGTATTATGA AGATGGAGCC 
CGGATTGAGG CTGCCTTCAG GAACTACATC CACCGAGCTG ATGCCAAGCA GGAGGAGGAC 
AGCTACGAGA TCTTCATATG TCACGCCAAT GTCATCCGTT ATATTGTTTG TAGGGCACTG 
CAGTTTCCCC CAGAAGGTTG GCTCCGCCTG TCCCTCAACA ATGGTAGTAT CACCCACCTG 
GTGATTCGAC CCAATGGTCG TGTTGCACTC AGGACCCTTG GGGACACAGG GTTCATGCCC 
CCAGACAAGA TTACTCGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondrial phosphatase PGAM5 regulates Keap1-mediated Bcl-xL degradation and controls cardiomyocyte apoptosis driven by myocardial ischemia/reperfusion injury.
In vitro cellular & developmental biology. Animal53(3)248-257(2017 Mar)
Yang C,Liu X,Yang F,Zhang W,Chen Z,Yan D,You Q,Wu X

The Ablation of Mitochondrial Protein Phosphatase Pgam5 Confers Resistance Against Metabolic Stress.
EBioMedicine582-92(2016 Mar)
Sekine S,Yao A,Hattori K,Sugawara S,Naguro I,Koike M,Uchiyama Y,Takeda K,Ichijo H

Selective removal of mitochondria via mitophagy: distinct pathways for different mitochondrial stresses.
Biochimica et biophysica acta1853(10 Pt B)2784-90(2015 Oct)
Wei H,Liu L,Chen Q

The Nrf2 regulatory network provides an interface between redox and intermediary metabolism.
Trends in biochemical sciences39(4)199-218(2014 Apr)
Hayes JD,Dinkova-Kostova AT

The mitochondrial phosphatase PGAM5 functions at the convergence point of multiple necrotic death pathways.
Cell148(1-2)228-43(2012 Jan)
Wang Z,Jiang H,Chen S,Du F,Wang X