Gart cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gart
Entrez Gene ID	288259
Full Name	phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
General protein information	Preferred Names phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase Names trifunctional purine biosynthetic protein adenosine-3 phosphoribosylglycinamide formyltransferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q11

mRNA and Protein(s)

mRNA	Protein	Name
XM_017597941.1	XP_017453430.1	trifunctional purine biosynthetic protein adenosine-3 isoform X1
NM_001011899.2	NP_001011899.2	trifunctional purine biosynthetic protein adenosine-3

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0A00957g	XP_451041.1
Pan troglodytes (chimpanzee)	GART	XP_003319102.1
Canis lupus familiaris (dog)	LOC487740	XP_544864.3
Gallus gallus (chicken)	GART	NP_001001469.1
Caenorhabditis elegans (roundworm)	F38B6.4	NP_509122.2
Schizosaccharomyces pombe (fission yeast)	ade1	NP_596304.1
Homo sapiens (human)	GART	NP_000810.1
Bos taurus (cattle)	GART	NP_001035563.1
Mus musculus (house mouse)	Gart	NP_034386.2
Drosophila melanogaster (fruit fly)	ade3	NP_523497.2
Oryza sativa (rice)	Os08g0191200	NP_001061170.1
Neurospora crassa	NCU00177	XP_956400.1
Canis lupus familiaris (dog)	LOC100856570	XP_005638901.1
Eremothecium gossypii	AGOS_AFR254C	NP_985801.2
Magnaporthe oryzae (rice blast fungus)	MGG_11343	XP_003713782.1
Oryza sativa (rice)	Os12g0197100	NP_001066357.1
Macaca mulatta (Rhesus monkey)	GART	XP_001093303.2
Danio rerio (zebrafish)	gart	NP_571692.1
Arabidopsis thaliana (thale cress)	AT1G09830	NP_172454.1
Xenopus tropicalis (tropical clawed frog)	gart	NP_001011441.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009786	XP_318881.4
Rattus norvegicus (Norway rat)	Gart	NP_001011899.2
Saccharomyces cerevisiae (baker's yeast)	ADE5,7	NP_011280.1

Gene Ontology
Bibliography

Related articles in PubMed

GART expression in rat spinal cord after injury and its role in inflammation.
Zhang D, Yue Y, Jiang S, Li A, Guo A, Wu X, Xia X, Cheng H, Zhang J, Tao T, Gu X
Brain research156441-51(2014 May)

Trafficking of intracellular folates.
Stover PJ, Field MS
Advances in nutrition (Bethesda, Md.)2(4)325-31(2011 Jul)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

Crystal structures of human GAR Tfase at low and high pH and with substrate beta-GAR.
Zhang Y, Desharnais J, Greasley SE, Beardsley GP, Boger DL, Wilson IA
Biochemistry41(48)14206-15(2002 Dec)

The human GARS-AIRS-GART gene encodes two proteins which are differentially expressed during human brain development and temporally overexpressed in cerebellum of individuals with Down syndrome.
Brodsky G, Barnes T, Bleskan J, Becker L, Cox M, Patterson D
Human molecular genetics6(12)2043-50(1997 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gart gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gart cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01467	NM_001011899.2 Latest version!	Rattus norvegicus phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (Gart), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa01467	XM_017597941.1 Latest version!	Rattus norvegicus phosphoribosylglycinamide formyltransferase (Gart), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gart ( NM_001011899.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001011899.2 Rattus norvegicus phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (Gart), mRNA.

Price & Availability↑

CloneID	ORa01467
Clone ID Related Accession (Same CDS sequence)	XM_017597941.1 , NM_001011899.2
Accession Version	NM_001011899.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527350400000
Organism	Rattus norvegicus(Norway rat)
Product	trifunctional purine biosynthetic protein adenosine-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473989.1. On Sep 4, 2008 this sequence version replaced NM_001011899.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGCCC GAGTTCTTGT AATCGGTAGT GGAGGAAGGG AACACACCCT GGCCTGGAAA 
CTCGCACAAT CCCCTCATGT CAAACAAGTG CTGGTTGCCC CAGGAAATGC AGGCACAGCT 
TCTGCTGGGA AGATCTCCAA TGCTGCTGTC TCCATCAATG ATCACACTGC TCTCGCTCGG 
TTCTGCAAAG ATGAAAAGAT TGAACTCGTA GTTGTTGGAC CAGAGGCCCC TCTGGCTGCA 
GGGATTGTTG GGGACCTGAC TTCTGCAGGA GTGCGATGCT TTGGCCCCAC GGCACAAGCA 
GCTCTACTGG AGTCCAGTAA AAAGTTTGCC AAAGAGTTTA TGGACCGACA TGGCGTCCCA 
ACTGCACAGT GGCGGGCATT CACCAGCCCT GAAGACGCCT GCAGCTTTAT TATGAGCGCG 
GACTTTCCTG CTTTGGTTGT GAAGGCCAGT GGCCTTGCAG CAGGGAAGGG GGTGATTGTG 
GCGAAGAGCA AAGCTGAAGC CTGCGAAGCT GTTCAGGAGA TCATGCAAGA AAAGTCATTT 
GGAGCAGCCG GTGAAACTGT TGTCGTGGAG GAGCTTCTGG AAGGGGAAGA AGTGTCTTGC 
CTGTGCTTCA CTGATGGGAA GACAGTGGCC CCAATGCCCC CAGCACAAGA CCATAAGCGA 
TTGCTGGATG GAGATCGGGG ACCCAACACT GGAGGAATGG GAGCCTACTG CCCTGCACCT 
CAGGTTCCTA AGGATTTGTT AGTAAAAATT AAAAACACAA TTCTTCAGAG AACAGTAGAT 
GGCATGCAGC AGGAGGGGGT GCCGTACACA GGAATTCTTT ATGCTGGTAT AATGCTGACC 
AAAGATGGCC CAAAAGTTTT GGAGTTTAAT TGCCGTTTTG GTGATCCAGA ATGCCAGGTC 
ATCCTCCCAC TTCTTAAAAG TGACCTTTAT GAAGTGATTC AGTCCACCTT TGATGGCCTG 
CTGAGCGAGT CTCCGCCTGT TTGGCTAGAA AACCACAGTG CCGTAACTGT TGTCATGGCG 
AGTGGAGGGT ATCCTGGAGC CTACACCAAG GGTGTGGAGA TTACAGGGTT TCCTGAGGCC 
CAAGCTTTAG GACTGCAGGT CTTTCATGCA GGCACTGCTC TTAAAGATGC CAAAGTGGTG 
ACCAGCGGGG GCAGAGTCCT CACAGTGACA GCTGTCCGGG AAAATCTCAT GTCAGCCCTT 
GATGAGGCCA GGAAGGGACT CGCTGCTCTA AAGTTTGAGG GAGCAGTTTA CCGAAAGGAC 
ATTGGCTTCC GTGCTGTGGC CTTCCTCCAG CGGCCCAGGG GCCTGACCTA CAAGGACTCT 
GGAGTAGACA TTGCAGCTGG AAATATGCTG GTTAAGAAAA TTCAGCCTTT AGCCAAAGCT 
ACCTCCAGAC CAGGCTGCAG TGTTGACCTT GGGGGCTTTG CTGGTCTTTT TGATTTGAAA 
GCAGCTGGTT TCAAAGATCC TCTTCTGGCC TCTGGAACAG ACGGTGTTGG AACTAAACTA 
AAGATTGCCC AGCTGTGCAA TAAGCATGAC TCCATCGGTC AGGATCTGGT TGCGATGTGC 
GTGAACGACA TCCTCGCACA AGGAGCAGAG CCCCTCTTCT TCCTTGACTA CTTTTCCTGT 
GGAAAACTTG ACCTTAGTAC AACTGAGGCT GTGGTTGCTG GAATTGCTGC AGCTTGCCGA 
CAGGCTGGGT GTGCTCTCCT AGGTGGGGAG ACAGCAGAGA TGCCCGACAT GTACCCCCCT 
GGAGAGTATG ATCTTGCCGG ATTTGCTGTT GGTGCTATGG AGAGACACCA GAAGCTCCCT 
CAGCTAGAAA GGATCGCCGA AGGAGATGCC GTTATTGGAG TGGCTTCATC TGGTCTTCAC 
AGCAATGGCT TTAGCCTTGT GAGGAAAATT GTGGAGAGGT CTTCACTCCA GTACTCCTCT 
CCAGCACCGG GTGGTTGTGG AGATCAGACT TTAGGGGACT TACTTCTAAC TCCAACCAGG 
ATCTACAGCC ATTCACTGCT GCCTATCATA CGTTCAGGAC GTGTCAAAGC TTTTGCTCAT 
ATTACTGGAG GTGGATTGCT GGAAAACATC CCCAGAGTCC TCCCTCGGAA GCTTGGAGTA 
GATTTAGATG CCTATACCTG GAGGGTCCCC AAGGTCTTCT CATGGCTACA GCAAGAAGGA 
CAGCTCTCTG AAGAAGAAAT GGCCAGGACA TTCAACTGTG GGGTTGGAGC TGCCCTGGTG 
GTATCAAAGG ACCAAACAGA GCAGATTCTC CATGACATTC GGCAGCACCA GGAAGACGCC 
TGGGTGATAG GCAGTGTCGT TGAGTGTCCC GAAGATTCCC CTCGTGTCAG AGTTAAGAAT 
CTGATTGAAA CCATGCAAAC AAAGGGGTCA GTGGTGACAA ACGGCTTCCT GAGAAGTAAT 
TTCCCTAGCC AGCAAAAGAA AGCAAGAGTG GCTGTCCTAA TATCTGGAAC AGGGTCGAAC 
CTCCAGGCAC TCATAGACAG CACTCGGGAT CCAAAAAGCT CCTCACACAT CGTTCTTGTC 
ATCTCCAACA AAGCTGCAGT TGCTGGCTTA GACCGAGCAG AGCGAGCTGG CATTCCCACC 
AGGGTGATTA ATCATAAGTT ATATAAAAGT CGTGTCGAGT TTGACAACGC AGTTGACCAT 
GTCCTGGAAG AGTTCTCAGT AGACATAGTC TGTCTCGCAG GATTCATGAG GATCCTCTCT 
GGCCCGTTTG TCAGAAAATG GGATGGGAAA ATGCTCAACA TCCACCCGTC CTTGCTCCCT 
TCTTTTAAGG GTTCAAATGC TCACGAGCAG GTTCTGGAGG CTGGAGTCAC CATTACTGGG 
TGCACCGTAC ACTTTGTAGC TGAAGACGTA GATGCCGGGC AAATCATCCT ACAGGAAGCT 
GTCCCTGTGC AGAGGGACGA CACTGTTGCA ACACTGTCTG AACGCGTCAA GGCAGCAGAA 
CATAAGATAT TTCCTGCAGC CCTCCAACTA GTGGCCAGTG GGGCTGTGCA GCTGGGAGAA 
GACGGCAAGA TCCACTGGGC CAGAGAGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011899.2
CDS	110..3142
Translation	MAARVLVIGSGGREHTLAWKLAQSPHVKQVLVAPGNAGTASAGKISNAAVSINDHTALARFCKDEKIELVVVGPEAPLAAGIVGDLTSAGVRCFGPTAQAALLESSKKFAKEFMDRHGVPTAQWRAFTSPEDACSFIMSADFPALVVKASGLAAGKGVIVAKSKAEACEAVQEIMQEKSFGAAGETVVVEELLEGEEVSCLCFTDGKTVAPMPPAQDHKRLLDGDRGPNTGGMGAYCPAPQVPKDLLVKIKNTILQRTVDGMQQEGVPYTGILYAGIMLTKDGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTFDGLLSESPPVWLENHSAVTVVMASGGYPGAYTKGVEITGFPEAQALGLQVFHAGTALKDAKVVTSGGRVLTVTAVRENLMSALDEARKGLAALKFEGAVYRKDIGFRAVAFLQRPRGLTYKDSGVDIAAGNMLVKKIQPLAKATSRPGCSVDLGGFAGLFDLKAAGFKDPLLASGTDGVGTKLKIAQLCNKHDSIGQDLVAMCVNDILAQGAEPLFFLDYFSCGKLDLSTTEAVVAGIAAACRQAGCALLGGETAEMPDMYPPGEYDLAGFAVGAMERHQKLPQLERIAEGDAVIGVASSGLHSNGFSLVRKIVERSSLQYSSPAPGGCGDQTLGDLLLTPTRIYSHSLLPIIRSGRVKAFAHITGGGLLENIPRVLPRKLGVDLDAYTWRVPKVFSWLQQEGQLSEEEMARTFNCGVGAALVVSKDQTEQILHDIRQHQEDAWVIGSVVECPEDSPRVRVKNLIETMQTKGSVVTNGFLRSNFPSQQKKARVAVLISGTGSNLQALIDSTRDPKSSSHIVLVISNKAAVAGLDRAERAGIPTRVINHKLYKSRVEFDNAVDHVLEEFSVDIVCLAGFMRILSGPFVRKWDGKMLNIHPSLLPSFKGSNAHEQVLEAGVTITGCTVHFVAEDVDAGQIILQEAVPVQRDDTVATLSERVKAAEHKIFPAALQLVASGAVQLGEDGKIHWAREQ

Target ORF information:

RefSeq Version	NM_001011899.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (Gart), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011899.2

ORF Insert Sequence:

ATGGCAGCCC GAGTTCTTGT AATCGGTAGT GGAGGAAGGG AACACACCCT GGCCTGGAAA 
CTCGCACAAT CCCCTCATGT CAAACAAGTG CTGGTTGCCC CAGGAAATGC AGGCACAGCT 
TCTGCTGGGA AGATCTCCAA TGCTGCTGTC TCCATCAATG ATCACACTGC TCTCGCTCGG 
TTCTGCAAAG ATGAAAAGAT TGAACTCGTA GTTGTTGGAC CAGAGGCCCC TCTGGCTGCA 
GGGATTGTTG GGGACCTGAC TTCTGCAGGA GTGCGATGCT TTGGCCCCAC GGCACAAGCA 
GCTCTACTGG AGTCCAGTAA AAAGTTTGCC AAAGAGTTTA TGGACCGACA TGGCGTCCCA 
ACTGCACAGT GGCGGGCATT CACCAGCCCT GAAGACGCCT GCAGCTTTAT TATGAGCGCG 
GACTTTCCTG CTTTGGTTGT GAAGGCCAGT GGCCTTGCAG CAGGGAAGGG GGTGATTGTG 
GCGAAGAGCA AAGCTGAAGC CTGCGAAGCT GTTCAGGAGA TCATGCAAGA AAAGTCATTT 
GGAGCAGCCG GTGAAACTGT TGTCGTGGAG GAGCTTCTGG AAGGGGAAGA AGTGTCTTGC 
CTGTGCTTCA CTGATGGGAA GACAGTGGCC CCAATGCCCC CAGCACAAGA CCATAAGCGA 
TTGCTGGATG GAGATCGGGG ACCCAACACT GGAGGAATGG GAGCCTACTG CCCTGCACCT 
CAGGTTCCTA AGGATTTGTT AGTAAAAATT AAAAACACAA TTCTTCAGAG AACAGTAGAT 
GGCATGCAGC AGGAGGGGGT GCCGTACACA GGAATTCTTT ATGCTGGTAT AATGCTGACC 
AAAGATGGCC CAAAAGTTTT GGAGTTTAAT TGCCGTTTTG GTGATCCAGA ATGCCAGGTC 
ATCCTCCCAC TTCTTAAAAG TGACCTTTAT GAAGTGATTC AGTCCACCTT TGATGGCCTG 
CTGAGCGAGT CTCCGCCTGT TTGGCTAGAA AACCACAGTG CCGTAACTGT TGTCATGGCG 
AGTGGAGGGT ATCCTGGAGC CTACACCAAG GGTGTGGAGA TTACAGGGTT TCCTGAGGCC 
CAAGCTTTAG GACTGCAGGT CTTTCATGCA GGCACTGCTC TTAAAGATGC CAAAGTGGTG 
ACCAGCGGGG GCAGAGTCCT CACAGTGACA GCTGTCCGGG AAAATCTCAT GTCAGCCCTT 
GATGAGGCCA GGAAGGGACT CGCTGCTCTA AAGTTTGAGG GAGCAGTTTA CCGAAAGGAC 
ATTGGCTTCC GTGCTGTGGC CTTCCTCCAG CGGCCCAGGG GCCTGACCTA CAAGGACTCT 
GGAGTAGACA TTGCAGCTGG AAATATGCTG GTTAAGAAAA TTCAGCCTTT AGCCAAAGCT 
ACCTCCAGAC CAGGCTGCAG TGTTGACCTT GGGGGCTTTG CTGGTCTTTT TGATTTGAAA 
GCAGCTGGTT TCAAAGATCC TCTTCTGGCC TCTGGAACAG ACGGTGTTGG AACTAAACTA 
AAGATTGCCC AGCTGTGCAA TAAGCATGAC TCCATCGGTC AGGATCTGGT TGCGATGTGC 
GTGAACGACA TCCTCGCACA AGGAGCAGAG CCCCTCTTCT TCCTTGACTA CTTTTCCTGT 
GGAAAACTTG ACCTTAGTAC AACTGAGGCT GTGGTTGCTG GAATTGCTGC AGCTTGCCGA 
CAGGCTGGGT GTGCTCTCCT AGGTGGGGAG ACAGCAGAGA TGCCCGACAT GTACCCCCCT 
GGAGAGTATG ATCTTGCCGG ATTTGCTGTT GGTGCTATGG AGAGACACCA GAAGCTCCCT 
CAGCTAGAAA GGATCGCCGA AGGAGATGCC GTTATTGGAG TGGCTTCATC TGGTCTTCAC 
AGCAATGGCT TTAGCCTTGT GAGGAAAATT GTGGAGAGGT CTTCACTCCA GTACTCCTCT 
CCAGCACCGG GTGGTTGTGG AGATCAGACT TTAGGGGACT TACTTCTAAC TCCAACCAGG 
ATCTACAGCC ATTCACTGCT GCCTATCATA CGTTCAGGAC GTGTCAAAGC TTTTGCTCAT 
ATTACTGGAG GTGGATTGCT GGAAAACATC CCCAGAGTCC TCCCTCGGAA GCTTGGAGTA 
GATTTAGATG CCTATACCTG GAGGGTCCCC AAGGTCTTCT CATGGCTACA GCAAGAAGGA 
CAGCTCTCTG AAGAAGAAAT GGCCAGGACA TTCAACTGTG GGGTTGGAGC TGCCCTGGTG 
GTATCAAAGG ACCAAACAGA GCAGATTCTC CATGACATTC GGCAGCACCA GGAAGACGCC 
TGGGTGATAG GCAGTGTCGT TGAGTGTCCC GAAGATTCCC CTCGTGTCAG AGTTAAGAAT 
CTGATTGAAA CCATGCAAAC AAAGGGGTCA GTGGTGACAA ACGGCTTCCT GAGAAGTAAT 
TTCCCTAGCC AGCAAAAGAA AGCAAGAGTG GCTGTCCTAA TATCTGGAAC AGGGTCGAAC 
CTCCAGGCAC TCATAGACAG CACTCGGGAT CCAAAAAGCT CCTCACACAT CGTTCTTGTC 
ATCTCCAACA AAGCTGCAGT TGCTGGCTTA GACCGAGCAG AGCGAGCTGG CATTCCCACC 
AGGGTGATTA ATCATAAGTT ATATAAAAGT CGTGTCGAGT TTGACAACGC AGTTGACCAT 
GTCCTGGAAG AGTTCTCAGT AGACATAGTC TGTCTCGCAG GATTCATGAG GATCCTCTCT 
GGCCCGTTTG TCAGAAAATG GGATGGGAAA ATGCTCAACA TCCACCCGTC CTTGCTCCCT 
TCTTTTAAGG GTTCAAATGC TCACGAGCAG GTTCTGGAGG CTGGAGTCAC CATTACTGGG 
TGCACCGTAC ACTTTGTAGC TGAAGACGTA GATGCCGGGC AAATCATCCT ACAGGAAGCT 
GTCCCTGTGC AGAGGGACGA CACTGTTGCA ACACTGTCTG AACGCGTCAA GGCAGCAGAA 
CATAAGATAT TTCCTGCAGC CCTCCAACTA GTGGCCAGTG GGGCTGTGCA GCTGGGAGAA 
GACGGCAAGA TCCACTGGGC CAGAGAGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gart ( XM_017597941.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453430.1 Rattus norvegicus phosphoribosylglycinamide formyltransferase (Gart), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa01467
Clone ID Related Accession (Same CDS sequence)	XM_017597941.1 , NM_001011899.2
Accession Version	XM_017597941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	trifunctional purine biosynthetic protein adenosine-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCC GAGTTCTTGT AATCGGTAGT GGAGGAAGGG AACACACCCT GGCCTGGAAA 
CTCGCACAAT CCCCTCATGT CAAACAAGTG CTGGTTGCCC CAGGAAATGC AGGCACAGCT 
TCTGCTGGGA AGATCTCCAA TGCTGCTGTC TCCATCAATG ATCACACTGC TCTCGCTCGG 
TTCTGCAAAG ATGAAAAGAT TGAACTCGTA GTTGTTGGAC CAGAGGCCCC TCTGGCTGCA 
GGGATTGTTG GGGACCTGAC TTCTGCAGGA GTGCGATGCT TTGGCCCCAC GGCACAAGCA 
GCTCTACTGG AGTCCAGTAA AAAGTTTGCC AAAGAGTTTA TGGACCGACA TGGCGTCCCA 
ACTGCACAGT GGCGGGCATT CACCAGCCCT GAAGACGCCT GCAGCTTTAT TATGAGCGCG 
GACTTTCCTG CTTTGGTTGT GAAGGCCAGT GGCCTTGCAG CAGGGAAGGG GGTGATTGTG 
GCGAAGAGCA AAGCTGAAGC CTGCGAAGCT GTTCAGGAGA TCATGCAAGA AAAGTCATTT 
GGAGCAGCCG GTGAAACTGT TGTCGTGGAG GAGCTTCTGG AAGGGGAAGA AGTGTCTTGC 
CTGTGCTTCA CTGATGGGAA GACAGTGGCC CCAATGCCCC CAGCACAAGA CCATAAGCGA 
TTGCTGGATG GAGATCGGGG ACCCAACACT GGAGGAATGG GAGCCTACTG CCCTGCACCT 
CAGGTTCCTA AGGATTTGTT AGTAAAAATT AAAAACACAA TTCTTCAGAG AACAGTAGAT 
GGCATGCAGC AGGAGGGGGT GCCGTACACA GGAATTCTTT ATGCTGGTAT AATGCTGACC 
AAAGATGGCC CAAAAGTTTT GGAGTTTAAT TGCCGTTTTG GTGATCCAGA ATGCCAGGTC 
ATCCTCCCAC TTCTTAAAAG TGACCTTTAT GAAGTGATTC AGTCCACCTT TGATGGCCTG 
CTGAGCGAGT CTCCGCCTGT TTGGCTAGAA AACCACAGTG CCGTAACTGT TGTCATGGCG 
AGTGGAGGGT ATCCTGGAGC CTACACCAAG GGTGTGGAGA TTACAGGGTT TCCTGAGGCC 
CAAGCTTTAG GACTGCAGGT CTTTCATGCA GGCACTGCTC TTAAAGATGC CAAAGTGGTG 
ACCAGCGGGG GCAGAGTCCT CACAGTGACA GCTGTCCGGG AAAATCTCAT GTCAGCCCTT 
GATGAGGCCA GGAAGGGACT CGCTGCTCTA AAGTTTGAGG GAGCAGTTTA CCGAAAGGAC 
ATTGGCTTCC GTGCTGTGGC CTTCCTCCAG CGGCCCAGGG GCCTGACCTA CAAGGACTCT 
GGAGTAGACA TTGCAGCTGG AAATATGCTG GTTAAGAAAA TTCAGCCTTT AGCCAAAGCT 
ACCTCCAGAC CAGGCTGCAG TGTTGACCTT GGGGGCTTTG CTGGTCTTTT TGATTTGAAA 
GCAGCTGGTT TCAAAGATCC TCTTCTGGCC TCTGGAACAG ACGGTGTTGG AACTAAACTA 
AAGATTGCCC AGCTGTGCAA TAAGCATGAC TCCATCGGTC AGGATCTGGT TGCGATGTGC 
GTGAACGACA TCCTCGCACA AGGAGCAGAG CCCCTCTTCT TCCTTGACTA CTTTTCCTGT 
GGAAAACTTG ACCTTAGTAC AACTGAGGCT GTGGTTGCTG GAATTGCTGC AGCTTGCCGA 
CAGGCTGGGT GTGCTCTCCT AGGTGGGGAG ACAGCAGAGA TGCCCGACAT GTACCCCCCT 
GGAGAGTATG ATCTTGCCGG ATTTGCTGTT GGTGCTATGG AGAGACACCA GAAGCTCCCT 
CAGCTAGAAA GGATCGCCGA AGGAGATGCC GTTATTGGAG TGGCTTCATC TGGTCTTCAC 
AGCAATGGCT TTAGCCTTGT GAGGAAAATT GTGGAGAGGT CTTCACTCCA GTACTCCTCT 
CCAGCACCGG GTGGTTGTGG AGATCAGACT TTAGGGGACT TACTTCTAAC TCCAACCAGG 
ATCTACAGCC ATTCACTGCT GCCTATCATA CGTTCAGGAC GTGTCAAAGC TTTTGCTCAT 
ATTACTGGAG GTGGATTGCT GGAAAACATC CCCAGAGTCC TCCCTCGGAA GCTTGGAGTA 
GATTTAGATG CCTATACCTG GAGGGTCCCC AAGGTCTTCT CATGGCTACA GCAAGAAGGA 
CAGCTCTCTG AAGAAGAAAT GGCCAGGACA TTCAACTGTG GGGTTGGAGC TGCCCTGGTG 
GTATCAAAGG ACCAAACAGA GCAGATTCTC CATGACATTC GGCAGCACCA GGAAGACGCC 
TGGGTGATAG GCAGTGTCGT TGAGTGTCCC GAAGATTCCC CTCGTGTCAG AGTTAAGAAT 
CTGATTGAAA CCATGCAAAC AAAGGGGTCA GTGGTGACAA ACGGCTTCCT GAGAAGTAAT 
TTCCCTAGCC AGCAAAAGAA AGCAAGAGTG GCTGTCCTAA TATCTGGAAC AGGGTCGAAC 
CTCCAGGCAC TCATAGACAG CACTCGGGAT CCAAAAAGCT CCTCACACAT CGTTCTTGTC 
ATCTCCAACA AAGCTGCAGT TGCTGGCTTA GACCGAGCAG AGCGAGCTGG CATTCCCACC 
AGGGTGATTA ATCATAAGTT ATATAAAAGT CGTGTCGAGT TTGACAACGC AGTTGACCAT 
GTCCTGGAAG AGTTCTCAGT AGACATAGTC TGTCTCGCAG GATTCATGAG GATCCTCTCT 
GGCCCGTTTG TCAGAAAATG GGATGGGAAA ATGCTCAACA TCCACCCGTC CTTGCTCCCT 
TCTTTTAAGG GTTCAAATGC TCACGAGCAG GTTCTGGAGG CTGGAGTCAC CATTACTGGG 
TGCACCGTAC ACTTTGTAGC TGAAGACGTA GATGCCGGGC AAATCATCCT ACAGGAAGCT 
GTCCCTGTGC AGAGGGACGA CACTGTTGCA ACACTGTCTG AACGCGTCAA GGCAGCAGAA 
CATAAGATAT TTCCTGCAGC CCTCCAACTA GTGGCCAGTG GGGCTGTGCA GCTGGGAGAA 
GACGGCAAGA TCCACTGGGC CAGAGAGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453430.1
CDS	164..3196
Translation	MAARVLVIGSGGREHTLAWKLAQSPHVKQVLVAPGNAGTASAGKISNAAVSINDHTALARFCKDEKIELVVVGPEAPLAAGIVGDLTSAGVRCFGPTAQAALLESSKKFAKEFMDRHGVPTAQWRAFTSPEDACSFIMSADFPALVVKASGLAAGKGVIVAKSKAEACEAVQEIMQEKSFGAAGETVVVEELLEGEEVSCLCFTDGKTVAPMPPAQDHKRLLDGDRGPNTGGMGAYCPAPQVPKDLLVKIKNTILQRTVDGMQQEGVPYTGILYAGIMLTKDGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTFDGLLSESPPVWLENHSAVTVVMASGGYPGAYTKGVEITGFPEAQALGLQVFHAGTALKDAKVVTSGGRVLTVTAVRENLMSALDEARKGLAALKFEGAVYRKDIGFRAVAFLQRPRGLTYKDSGVDIAAGNMLVKKIQPLAKATSRPGCSVDLGGFAGLFDLKAAGFKDPLLASGTDGVGTKLKIAQLCNKHDSIGQDLVAMCVNDILAQGAEPLFFLDYFSCGKLDLSTTEAVVAGIAAACRQAGCALLGGETAEMPDMYPPGEYDLAGFAVGAMERHQKLPQLERIAEGDAVIGVASSGLHSNGFSLVRKIVERSSLQYSSPAPGGCGDQTLGDLLLTPTRIYSHSLLPIIRSGRVKAFAHITGGGLLENIPRVLPRKLGVDLDAYTWRVPKVFSWLQQEGQLSEEEMARTFNCGVGAALVVSKDQTEQILHDIRQHQEDAWVIGSVVECPEDSPRVRVKNLIETMQTKGSVVTNGFLRSNFPSQQKKARVAVLISGTGSNLQALIDSTRDPKSSSHIVLVISNKAAVAGLDRAERAGIPTRVINHKLYKSRVEFDNAVDHVLEEFSVDIVCLAGFMRILSGPFVRKWDGKMLNIHPSLLPSFKGSNAHEQVLEAGVTITGCTVHFVAEDVDAGQIILQEAVPVQRDDTVATLSERVKAAEHKIFPAALQLVASGAVQLGEDGKIHWAREQ

Target ORF information:

RefSeq Version	XM_017597941.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoribosylglycinamide formyltransferase (Gart), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597941.1

ORF Insert Sequence:

ATGGCAGCCC GAGTTCTTGT AATCGGTAGT GGAGGAAGGG AACACACCCT GGCCTGGAAA 
CTCGCACAAT CCCCTCATGT CAAACAAGTG CTGGTTGCCC CAGGAAATGC AGGCACAGCT 
TCTGCTGGGA AGATCTCCAA TGCTGCTGTC TCCATCAATG ATCACACTGC TCTCGCTCGG 
TTCTGCAAAG ATGAAAAGAT TGAACTCGTA GTTGTTGGAC CAGAGGCCCC TCTGGCTGCA 
GGGATTGTTG GGGACCTGAC TTCTGCAGGA GTGCGATGCT TTGGCCCCAC GGCACAAGCA 
GCTCTACTGG AGTCCAGTAA AAAGTTTGCC AAAGAGTTTA TGGACCGACA TGGCGTCCCA 
ACTGCACAGT GGCGGGCATT CACCAGCCCT GAAGACGCCT GCAGCTTTAT TATGAGCGCG 
GACTTTCCTG CTTTGGTTGT GAAGGCCAGT GGCCTTGCAG CAGGGAAGGG GGTGATTGTG 
GCGAAGAGCA AAGCTGAAGC CTGCGAAGCT GTTCAGGAGA TCATGCAAGA AAAGTCATTT 
GGAGCAGCCG GTGAAACTGT TGTCGTGGAG GAGCTTCTGG AAGGGGAAGA AGTGTCTTGC 
CTGTGCTTCA CTGATGGGAA GACAGTGGCC CCAATGCCCC CAGCACAAGA CCATAAGCGA 
TTGCTGGATG GAGATCGGGG ACCCAACACT GGAGGAATGG GAGCCTACTG CCCTGCACCT 
CAGGTTCCTA AGGATTTGTT AGTAAAAATT AAAAACACAA TTCTTCAGAG AACAGTAGAT 
GGCATGCAGC AGGAGGGGGT GCCGTACACA GGAATTCTTT ATGCTGGTAT AATGCTGACC 
AAAGATGGCC CAAAAGTTTT GGAGTTTAAT TGCCGTTTTG GTGATCCAGA ATGCCAGGTC 
ATCCTCCCAC TTCTTAAAAG TGACCTTTAT GAAGTGATTC AGTCCACCTT TGATGGCCTG 
CTGAGCGAGT CTCCGCCTGT TTGGCTAGAA AACCACAGTG CCGTAACTGT TGTCATGGCG 
AGTGGAGGGT ATCCTGGAGC CTACACCAAG GGTGTGGAGA TTACAGGGTT TCCTGAGGCC 
CAAGCTTTAG GACTGCAGGT CTTTCATGCA GGCACTGCTC TTAAAGATGC CAAAGTGGTG 
ACCAGCGGGG GCAGAGTCCT CACAGTGACA GCTGTCCGGG AAAATCTCAT GTCAGCCCTT 
GATGAGGCCA GGAAGGGACT CGCTGCTCTA AAGTTTGAGG GAGCAGTTTA CCGAAAGGAC 
ATTGGCTTCC GTGCTGTGGC CTTCCTCCAG CGGCCCAGGG GCCTGACCTA CAAGGACTCT 
GGAGTAGACA TTGCAGCTGG AAATATGCTG GTTAAGAAAA TTCAGCCTTT AGCCAAAGCT 
ACCTCCAGAC CAGGCTGCAG TGTTGACCTT GGGGGCTTTG CTGGTCTTTT TGATTTGAAA 
GCAGCTGGTT TCAAAGATCC TCTTCTGGCC TCTGGAACAG ACGGTGTTGG AACTAAACTA 
AAGATTGCCC AGCTGTGCAA TAAGCATGAC TCCATCGGTC AGGATCTGGT TGCGATGTGC 
GTGAACGACA TCCTCGCACA AGGAGCAGAG CCCCTCTTCT TCCTTGACTA CTTTTCCTGT 
GGAAAACTTG ACCTTAGTAC AACTGAGGCT GTGGTTGCTG GAATTGCTGC AGCTTGCCGA 
CAGGCTGGGT GTGCTCTCCT AGGTGGGGAG ACAGCAGAGA TGCCCGACAT GTACCCCCCT 
GGAGAGTATG ATCTTGCCGG ATTTGCTGTT GGTGCTATGG AGAGACACCA GAAGCTCCCT 
CAGCTAGAAA GGATCGCCGA AGGAGATGCC GTTATTGGAG TGGCTTCATC TGGTCTTCAC 
AGCAATGGCT TTAGCCTTGT GAGGAAAATT GTGGAGAGGT CTTCACTCCA GTACTCCTCT 
CCAGCACCGG GTGGTTGTGG AGATCAGACT TTAGGGGACT TACTTCTAAC TCCAACCAGG 
ATCTACAGCC ATTCACTGCT GCCTATCATA CGTTCAGGAC GTGTCAAAGC TTTTGCTCAT 
ATTACTGGAG GTGGATTGCT GGAAAACATC CCCAGAGTCC TCCCTCGGAA GCTTGGAGTA 
GATTTAGATG CCTATACCTG GAGGGTCCCC AAGGTCTTCT CATGGCTACA GCAAGAAGGA 
CAGCTCTCTG AAGAAGAAAT GGCCAGGACA TTCAACTGTG GGGTTGGAGC TGCCCTGGTG 
GTATCAAAGG ACCAAACAGA GCAGATTCTC CATGACATTC GGCAGCACCA GGAAGACGCC 
TGGGTGATAG GCAGTGTCGT TGAGTGTCCC GAAGATTCCC CTCGTGTCAG AGTTAAGAAT 
CTGATTGAAA CCATGCAAAC AAAGGGGTCA GTGGTGACAA ACGGCTTCCT GAGAAGTAAT 
TTCCCTAGCC AGCAAAAGAA AGCAAGAGTG GCTGTCCTAA TATCTGGAAC AGGGTCGAAC 
CTCCAGGCAC TCATAGACAG CACTCGGGAT CCAAAAAGCT CCTCACACAT CGTTCTTGTC 
ATCTCCAACA AAGCTGCAGT TGCTGGCTTA GACCGAGCAG AGCGAGCTGG CATTCCCACC 
AGGGTGATTA ATCATAAGTT ATATAAAAGT CGTGTCGAGT TTGACAACGC AGTTGACCAT 
GTCCTGGAAG AGTTCTCAGT AGACATAGTC TGTCTCGCAG GATTCATGAG GATCCTCTCT 
GGCCCGTTTG TCAGAAAATG GGATGGGAAA ATGCTCAACA TCCACCCGTC CTTGCTCCCT 
TCTTTTAAGG GTTCAAATGC TCACGAGCAG GTTCTGGAGG CTGGAGTCAC CATTACTGGG 
TGCACCGTAC ACTTTGTAGC TGAAGACGTA GATGCCGGGC AAATCATCCT ACAGGAAGCT 
GTCCCTGTGC AGAGGGACGA CACTGTTGCA ACACTGTCTG AACGCGTCAA GGCAGCAGAA 
CATAAGATAT TTCCTGCAGC CCTCCAACTA GTGGCCAGTG GGGCTGTGCA GCTGGGAGAA 
GACGGCAAGA TCCACTGGGC CAGAGAGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GART expression in rat spinal cord after injury and its role in inflammation.
Brain research156441-51(2014 May)
Zhang D,Yue Y,Jiang S,Li A,Guo A,Wu X,Xia X,Cheng H,Zhang J,Tao T,Gu X

Trafficking of intracellular folates.
Advances in nutrition (Bethesda, Md.)2(4)325-31(2011 Jul)
Stover PJ,Field MS

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

Crystal structures of human GAR Tfase at low and high pH and with substrate beta-GAR.
Biochemistry41(48)14206-15(2002 Dec)
Zhang Y,Desharnais J,Greasley SE,Beardsley GP,Boger DL,Wilson IA

The human GARS-AIRS-GART gene encodes two proteins which are differentially expressed during human brain development and temporally overexpressed in cerebellum of individuals with Down syndrome.
Human molecular genetics6(12)2043-50(1997 Nov)
Brodsky G,Barnes T,Bleskan J,Becker L,Cox M,Patterson D

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Gart cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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