Chtf18 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chtf18
Entrez Gene ID	287146
Full Name	chromosome transmission fidelity factor 18
General protein information	Preferred Names chromosome transmission fidelity factor 18 Names chromosome transmission fidelity protein 18 homolog CTF18, chromosome transmission fidelity factor 18 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_006245936.3	XP_006245998.1	chromosome transmission fidelity protein 18 homolog isoform X1
NM_001105773.1	NP_001099243.1	chromosome transmission fidelity protein 18 homolog
XM_017597066.1	XP_017452555.1	chromosome transmission fidelity protein 18 homolog isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Chtf18	NP_001099243.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008869	XP_319609.4
Oryza sativa (rice)	Os03g0264800	NP_001049644.2
Macaca mulatta (Rhesus monkey)	CHTF18	XP_001087813.1
Bos taurus (cattle)	CHTF18	NP_001179389.1
Mus musculus (house mouse)	Chtf18	NP_663384.2
Pan troglodytes (chimpanzee)	CHTF18	XP_003952800.1
Canis lupus familiaris (dog)	CHTF18	XP_547205.4
Gallus gallus (chicken)	CHTF18	NP_001264989.1
Danio rerio (zebrafish)	chtf18	NP_001103572.2
Arabidopsis thaliana (thale cress)	CTF18	NP_171966.2
Homo sapiens (human)	CHTF18	NP_071375.1
Drosophila melanogaster (fruit fly)	cutlet	NP_787969.1
Xenopus tropicalis (tropical clawed frog)	chtf18	XP_002935905.2

Gene Ontology
Bibliography

Related articles in PubMed

Disruption of CHTF18 causes defective meiotic recombination in male mice.
Berkowitz KM, Sowash AR, Koenig LR, Urcuyo D, Khan F, Yang F, Wang PJ, Jongens TA, Kaestner KH
PLoS genetics8(11)e1002996(2012)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Germline expression of mammalian CTF18, an evolutionarily conserved protein required for germ cell proliferation in the fly and sister chromatid cohesion in yeast.
Berkowitz KM, Kaestner KH, Jongens TA
Molecular human reproduction14(3)143-50(2008 Mar)

The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA.
Bermudez VP, Maniwa Y, Tappin I, Ozato K, Yokomori K, Hurwitz J
Proceedings of the National Academy of Sciences of the United States of America100(18)10237-42(2003 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Chtf18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chtf18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11382	NM_001105773.1 Latest version!	Rattus norvegicus chromosome transmission fidelity factor 18 (Chtf18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa32854	XM_006245936.3 Latest version!	Rattus norvegicus chromosome transmission fidelity factor 18 (Chtf18), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa57208	XM_017597066.1 Latest version!	Rattus norvegicus chromosome transmission fidelity factor 18 (Chtf18), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chtf18 ( NM_001105773.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099243.1 Rattus norvegicus chromosome transmission fidelity factor 18 (Chtf18), mRNA.

Price & Availability↑

CloneID	ORa11382
Clone ID Related Accession (Same CDS sequence)	NM_001105773.1
Accession Version	NM_001105773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Rattus norvegicus(Norway rat)
Product	chromosome transmission fidelity protein 18 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473948.1. On or before Oct 3, 2007 this sequence version replaced XM_213250.4, XM_001060640.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGACT ACGAGGAAGA TCTGTATGGT GTGGAGGATG ACTTCCAAAA TCAGTTCGCG 
GCCGAACTCG AAGTGCTGGC GGAGCTGGAA GGAACAGGGG ACCTGGCGCC TTCTGAGACC 
CTCCAGACTC CGGCGAGCCG CCCCCCGCTG ACCTTCGAAG AGGCCATCGC TGGTGGGGAC 
ATTGTCCCAC GTCCTTGCCC TGCAGGGTCC TCAGGAAATG ACAGCCGCAA TACCAGAAAG 
AATGTTCGTA GGGACCAGCC CGCGCCCTCC AGCCCCATGG TCAAACGGCC CAGGTTGGAC 
GTGGTGAAGA GGCTGAACTT CGAGCCAGAT AAAGAGGAGC TCTTGTACCC CGATTCTCCT 
CCTGGGGACA TCACCCCCCC ACCGAGTCCA GAGGTCTTCC CTGAGATGTG GGATGCTGGG 
CCCTCAGATG CTGGTGCTGA CAATGGCCTC ATGCAGGCAT TGCCAAGTCC CCGCAATCCC 
GTTCTGAGAC GGCCCCCTGT CTTAGAGGAT TACATCAATG TGACGTCCAC GTGTGGAGAG 
CGGGCCTTTT TGGTGCTTCG GGCTGACCTC ACAGGCACTG GGGTGCAGAA CCATCTTCCT 
GATGTCCAGT GGCGAGGCCG TGGCCAGCTG GACCTTCTGG GTGTCCCCTT CACCTCCCTG 
AAGGAGCAAG TTGACAACAA GAGACGGCAG CAACTGCTTG AGGAGGCCCA ACAGCTTTCA 
GACACTCTAC ATAGCCTCAG GTCTGAAGGG GAAGAGGCCA TGCTTGAGGG GCCCCCTGAG 
GAGGAGCCGG GCCCTGGCCA GGAGACTGCT CAGCACTGCC TCTGGGTGGA CGAGTTTGCA 
CCCCAGCACT ACACAGAACT GCTCAGTGAT GACTTTACCA ACCGCTGCCT TCTCAAATGG 
CTGAAGCTAT GGGACCTTGT GGTGTTTGGT CATGAGAGGC CTGCCCGGAA GCCCAGACCT 
GGTGTAGAGC CCACCCGGGT TGGGAAGGAG GCCCCAGCCC CTGGCAAGTG GAAGAGTCAT 
GAGCAGGTAC TGGAGGAAAT GCTAGAAGCT GAGCTGGACC CGAGCCGGAG GCCTCGGCAG 
AAGGTGGCAC TGCTGTGTGG CCCTCCAGGA TTAGGCAAGA CCACGCTGGC TCATGTGGTC 
GCAAGGCATG CTGGGTATTG CGTGGTAGAG ATGAATGCAA GTGATGACCG TAGCCCCGAG 
GCCTTCCGTA CACGCATTGA AGCAGCTACA CAAATGGAGT CGGTGCTAGG TGCCGGTGGG 
AGGCCCAACT GCTTGGTTAT CGATGAGATT GATGGAGCCC CCACGGCGGC GATCAACGTT 
CTCTTGAGCA TCTTGAATCG CAAGGGCCCG CAGGAGGCTG AGCAAGGGGG CACAGCTGTG 
GCTGCGGCAG GGGGACGGCG ACGCAGGGCT GAGGGGGGCC TCCTGACCAG GCCTATCATC 
TGCATCTGCA ATGATCAGTT CACACCTTCT TTGAGGCAGC TGAAGCAGCA GGCTCTTCTG 
CTCCACGTCC CACCAACTCT GCCCTCCAGG CTGGTGCAGC GGCTCCAGGA GATCTCCCTG 
CAGCACGGCA TGCGGTCTGA CCCGGGTGCA CTGGCTGCCC TCTGTGAGAA GACAGACAAT 
GACATCCGTG CCTGCATCAA CACACTACAG TTTCTGTATG GGAGGGGCCA GCGGGAGCTG 
AGCGTGAAGG ATGTGCAGAC CACCCATGTC GGCCTGAAAG ACCAGCGCAA GGGGCTATTC 
TCTGTGTGGC AAGAGGTCTT CCAGTTGCCC AGGGCTCAAA GGCGACTTGT GGGCCAGGAC 
CTGATGCTAC CAACCCATGC TCTCCTGCTC AATGACAGTG ACAAGGGCTC TCTGACCTTG 
GCTTCCCAGC GCTTCTACCA CATCCTGCGT GTGACCACTT CTGCAGGCGA GCATGAGAAG 
GTTGTGCAGG GCCTGTTTGA CAACTTTCTA CGCCTGCGGC TTCGGGACTC CAGCCTGGGC 
ACCGTGTGCT GTGCCCTTGA CTGGCTGGCC TTCGATGACC TGCTGGAACA GGCGGCCCAC 
CACGGCCAGA GCTTTCAGCT GCTGCGTTAC TTGCCCTTCC TGCCCGCGGC CTTCCACGTG 
CTCTTCGCCT CCAGCCACGT GCCGCGCATC ACTTTCCCCA GCAGCCAGCA GGAGGCCCAG 
ACCCGGTTGA GCCAGACAAG GAACCACATA CAGACTCTGG TGTCAGGAAT GGCACCAGCC 
ACTCGCAGCC GGGCCACCCC GCAGGCTCTT GTTCTAGATA CTCTCTGCCT GCTCCTGGAT 
GTCCTCTCGC CTAAGCTGCG CCCCGTGAGC ACACAGCTGT ACAGTGCCCG TGAGAAGCAG 
CAGTTGTCGA GCCTTGTGGG CACCATGCTT GCCTATAGCC TCACCTACCA CCAGGAGCGC 
ACACCTGATG GGCAGTACCT CTACAGGCTG GAGCCGAATG TGGAGGAGGT CTGCCGCTTC 
CCAGAACTAC CTGCCCGCAA GCCCCTCACC TACCAGGCTA AGCAGCTTAT TGCCCGGGAG 
ATTGAAATGG AGAAGATGTG GAGGGCAGAG GCTTTGGCCT GGACTGGAGG TGGCCCTCAG 
GTGGACCAGG GTCCCTCAGG GCCTGCAAAT CTGCGGACGG ACAGTGGGGA AAAGGGGACG 
AGGCAGCCTG CCCCCCGTAA CCATGAGCAG CGGCTAGAAC ACATCATGAA GAGAGCAGCC 
GTGCAGGAGC AGCCTGAGAG GGACTTCTTT GGACGTGTGG TCATCAGGAA AGTGGCAGTC 
CCAAGCAAAG AGGTTGAGGC CCCCCAGAAG GACACGGACG AGTGGCGCAT GGGTGTGGCA 
GTGGGCAGGA GTGAGGTGTG GTTCCGGTTC AACGAGGGTG TCTCCAATGC TGTTCGGCGC 
AGCCTGTACA TCAGGGACCT GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099243.1
CDS	76..2982
Translation	MEDYEEDLYGVEDDFQNQFAAELEVLAELEGTGDLAPSETLQTPASRPPLTFEEAIAGGDIVPRPCPAGSSGNDSRNTRKNVRRDQPAPSSPMVKRPRLDVVKRLNFEPDKEELLYPDSPPGDITPPPSPEVFPEMWDAGPSDAGADNGLMQALPSPRNPVLRRPPVLEDYINVTSTCGERAFLVLRADLTGTGVQNHLPDVQWRGRGQLDLLGVPFTSLKEQVDNKRRQQLLEEAQQLSDTLHSLRSEGEEAMLEGPPEEEPGPGQETAQHCLWVDEFAPQHYTELLSDDFTNRCLLKWLKLWDLVVFGHERPARKPRPGVEPTRVGKEAPAPGKWKSHEQVLEEMLEAELDPSRRPRQKVALLCGPPGLGKTTLAHVVARHAGYCVVEMNASDDRSPEAFRTRIEAATQMESVLGAGGRPNCLVIDEIDGAPTAAINVLLSILNRKGPQEAEQGGTAVAAAGGRRRRAEGGLLTRPIICICNDQFTPSLRQLKQQALLLHVPPTLPSRLVQRLQEISLQHGMRSDPGALAALCEKTDNDIRACINTLQFLYGRGQRELSVKDVQTTHVGLKDQRKGLFSVWQEVFQLPRAQRRLVGQDLMLPTHALLLNDSDKGSLTLASQRFYHILRVTTSAGEHEKVVQGLFDNFLRLRLRDSSLGTVCCALDWLAFDDLLEQAAHHGQSFQLLRYLPFLPAAFHVLFASSHVPRITFPSSQQEAQTRLSQTRNHIQTLVSGMAPATRSRATPQALVLDTLCLLLDVLSPKLRPVSTQLYSAREKQQLSSLVGTMLAYSLTYHQERTPDGQYLYRLEPNVEEVCRFPELPARKPLTYQAKQLIAREIEMEKMWRAEALAWTGGGPQVDQGPSGPANLRTDSGEKGTRQPAPRNHEQRLEHIMKRAAVQEQPERDFFGRVVIRKVAVPSKEVEAPQKDTDEWRMGVAVGRSEVWFRFNEGVSNAVRRSLYIRDLL

Target ORF information:

RefSeq Version	NM_001105773.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chromosome transmission fidelity factor 18 (Chtf18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105773.1

ORF Insert Sequence:

ATGGAAGACT ACGAGGAAGA TCTGTATGGT GTGGAGGATG ACTTCCAAAA TCAGTTCGCG 
GCCGAACTCG AAGTGCTGGC GGAGCTGGAA GGAACAGGGG ACCTGGCGCC TTCTGAGACC 
CTCCAGACTC CGGCGAGCCG CCCCCCGCTG ACCTTCGAAG AGGCCATCGC TGGTGGGGAC 
ATTGTCCCAC GTCCTTGCCC TGCAGGGTCC TCAGGAAATG ACAGCCGCAA TACCAGAAAG 
AATGTTCGTA GGGACCAGCC CGCGCCCTCC AGCCCCATGG TCAAACGGCC CAGGTTGGAC 
GTGGTGAAGA GGCTGAACTT CGAGCCAGAT AAAGAGGAGC TCTTGTACCC CGATTCTCCT 
CCTGGGGACA TCACCCCCCC ACCGAGTCCA GAGGTCTTCC CTGAGATGTG GGATGCTGGG 
CCCTCAGATG CTGGTGCTGA CAATGGCCTC ATGCAGGCAT TGCCAAGTCC CCGCAATCCC 
GTTCTGAGAC GGCCCCCTGT CTTAGAGGAT TACATCAATG TGACGTCCAC GTGTGGAGAG 
CGGGCCTTTT TGGTGCTTCG GGCTGACCTC ACAGGCACTG GGGTGCAGAA CCATCTTCCT 
GATGTCCAGT GGCGAGGCCG TGGCCAGCTG GACCTTCTGG GTGTCCCCTT CACCTCCCTG 
AAGGAGCAAG TTGACAACAA GAGACGGCAG CAACTGCTTG AGGAGGCCCA ACAGCTTTCA 
GACACTCTAC ATAGCCTCAG GTCTGAAGGG GAAGAGGCCA TGCTTGAGGG GCCCCCTGAG 
GAGGAGCCGG GCCCTGGCCA GGAGACTGCT CAGCACTGCC TCTGGGTGGA CGAGTTTGCA 
CCCCAGCACT ACACAGAACT GCTCAGTGAT GACTTTACCA ACCGCTGCCT TCTCAAATGG 
CTGAAGCTAT GGGACCTTGT GGTGTTTGGT CATGAGAGGC CTGCCCGGAA GCCCAGACCT 
GGTGTAGAGC CCACCCGGGT TGGGAAGGAG GCCCCAGCCC CTGGCAAGTG GAAGAGTCAT 
GAGCAGGTAC TGGAGGAAAT GCTAGAAGCT GAGCTGGACC CGAGCCGGAG GCCTCGGCAG 
AAGGTGGCAC TGCTGTGTGG CCCTCCAGGA TTAGGCAAGA CCACGCTGGC TCATGTGGTC 
GCAAGGCATG CTGGGTATTG CGTGGTAGAG ATGAATGCAA GTGATGACCG TAGCCCCGAG 
GCCTTCCGTA CACGCATTGA AGCAGCTACA CAAATGGAGT CGGTGCTAGG TGCCGGTGGG 
AGGCCCAACT GCTTGGTTAT CGATGAGATT GATGGAGCCC CCACGGCGGC GATCAACGTT 
CTCTTGAGCA TCTTGAATCG CAAGGGCCCG CAGGAGGCTG AGCAAGGGGG CACAGCTGTG 
GCTGCGGCAG GGGGACGGCG ACGCAGGGCT GAGGGGGGCC TCCTGACCAG GCCTATCATC 
TGCATCTGCA ATGATCAGTT CACACCTTCT TTGAGGCAGC TGAAGCAGCA GGCTCTTCTG 
CTCCACGTCC CACCAACTCT GCCCTCCAGG CTGGTGCAGC GGCTCCAGGA GATCTCCCTG 
CAGCACGGCA TGCGGTCTGA CCCGGGTGCA CTGGCTGCCC TCTGTGAGAA GACAGACAAT 
GACATCCGTG CCTGCATCAA CACACTACAG TTTCTGTATG GGAGGGGCCA GCGGGAGCTG 
AGCGTGAAGG ATGTGCAGAC CACCCATGTC GGCCTGAAAG ACCAGCGCAA GGGGCTATTC 
TCTGTGTGGC AAGAGGTCTT CCAGTTGCCC AGGGCTCAAA GGCGACTTGT GGGCCAGGAC 
CTGATGCTAC CAACCCATGC TCTCCTGCTC AATGACAGTG ACAAGGGCTC TCTGACCTTG 
GCTTCCCAGC GCTTCTACCA CATCCTGCGT GTGACCACTT CTGCAGGCGA GCATGAGAAG 
GTTGTGCAGG GCCTGTTTGA CAACTTTCTA CGCCTGCGGC TTCGGGACTC CAGCCTGGGC 
ACCGTGTGCT GTGCCCTTGA CTGGCTGGCC TTCGATGACC TGCTGGAACA GGCGGCCCAC 
CACGGCCAGA GCTTTCAGCT GCTGCGTTAC TTGCCCTTCC TGCCCGCGGC CTTCCACGTG 
CTCTTCGCCT CCAGCCACGT GCCGCGCATC ACTTTCCCCA GCAGCCAGCA GGAGGCCCAG 
ACCCGGTTGA GCCAGACAAG GAACCACATA CAGACTCTGG TGTCAGGAAT GGCACCAGCC 
ACTCGCAGCC GGGCCACCCC GCAGGCTCTT GTTCTAGATA CTCTCTGCCT GCTCCTGGAT 
GTCCTCTCGC CTAAGCTGCG CCCCGTGAGC ACACAGCTGT ACAGTGCCCG TGAGAAGCAG 
CAGTTGTCGA GCCTTGTGGG CACCATGCTT GCCTATAGCC TCACCTACCA CCAGGAGCGC 
ACACCTGATG GGCAGTACCT CTACAGGCTG GAGCCGAATG TGGAGGAGGT CTGCCGCTTC 
CCAGAACTAC CTGCCCGCAA GCCCCTCACC TACCAGGCTA AGCAGCTTAT TGCCCGGGAG 
ATTGAAATGG AGAAGATGTG GAGGGCAGAG GCTTTGGCCT GGACTGGAGG TGGCCCTCAG 
GTGGACCAGG GTCCCTCAGG GCCTGCAAAT CTGCGGACGG ACAGTGGGGA AAAGGGGACG 
AGGCAGCCTG CCCCCCGTAA CCATGAGCAG CGGCTAGAAC ACATCATGAA GAGAGCAGCC 
GTGCAGGAGC AGCCTGAGAG GGACTTCTTT GGACGTGTGG TCATCAGGAA AGTGGCAGTC 
CCAAGCAAAG AGGTTGAGGC CCCCCAGAAG GACACGGACG AGTGGCGCAT GGGTGTGGCA 
GTGGGCAGGA GTGAGGTGTG GTTCCGGTTC AACGAGGGTG TCTCCAATGC TGTTCGGCGC 
AGCCTGTACA TCAGGGACCT GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chtf18 ( XM_006245936.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245998.1 Rattus norvegicus chromosome transmission fidelity factor 18 (Chtf18), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32854
Clone ID Related Accession (Same CDS sequence)	XM_006245936.3
Accession Version	XM_006245936.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	chromosome transmission fidelity protein 18 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245936.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACT ACGAGGAAGA TCTGTATGGT GTGGAGGATG ACTTCCAAAA TCAGTTCGCG 
GCCGAACTCG AAGTGCTGGC GGAGCTGGAA GCAGGAACAG GGGACCTGGC GCCTTCTGAG 
ACCCTCCAGA CTCCGGCGAG CCGCCCCCCG CTGACCTTCG AAGAGGCCAT CGCTGGTGGG 
GACATTGTCC CACGTCCTTG CCCTGCAGGG TCCTCAGGAA ATGACAGCCG CAATACCAGA 
AAGAATGTTC GTAGGGACCA GCCCGCGCCC TCCAGCCCCA TGGTCAAACG GCCCAGGTTG 
GACGTGGTGA AGAGGCTGAA CTTCGAGCCA GATAAAGAGG AGCTCTTGTA CCCCGATTCT 
CCTCCTGGGG ACATCACCCC CCCACCGAGT CCAGAGGTCT TCCCTGAGAT GTGGGATGCT 
GGGCCCTCAG ATGCTGGTGC TGACAATGGC CTCATGCAGG CATTGCCAAG TCCCCGCAAT 
CCCGTTCTGA GACGGCCCCC TGTCTTAGAG GATTACATCA ATGTGACGTC CACGTGTGGA 
GAGCGGGCCT TTTTGGTGCT TCGGGCTGAC CTCACAGGCA CTGGGGTGCA GAACCATCTT 
CCTGATGTCC AGTGGCGAGG CCGTGGCCAG CTGGACCTTC TGGGTGTCCC CTTCACCTCC 
CTGAAGGAGC AAGTTGACAA CAAGAGACGG CAGCAACTGC TTGAGGAGGC CCAACAGCTT 
TCAGACACTC TACATAGCCT CAGGTCTGAA GGGGAAGAGG CCATGCTTGA GGGGCCCCCT 
GAGGAGGAGC CGGGCCCTGG CCAGGAGACT GCTCAGCACT GCCTCTGGGT GGACGAGTTT 
GCACCCCAGC ACTACACAGA ACTGCTCAGT GATGACTTTA CCAACCGCTG CCTTCTCAAA 
TGGCTGAAGC TATGGGACCT TGTGGTGTTT GGTCATGAGA GGCCTGCCCG GAAGCCCAGA 
CCTGGTGTAG AGCCCACCCG GGTTGGGAAG GAGGCCCCAG CCCCTGGCAA GTGGAAGAGT 
CATGAGCAGG TACTGGAGGA AATGCTAGAA GCTGAGCTGG ACCCGAGCCG GAGGCCTCGG 
CAGAAGGTGG CACTGCTGTG TGGCCCTCCA GGATTAGGCA AGACCACGCT GGCTCATGTG 
GTCGCAAGGC ATGCTGGGTA TTGCGTGGTA GAGATGAATG CAAGTGATGA CCGTAGCCCC 
GAGGCCTTCC GTACACGCAT TGAAGCAGCT ACACAAATGG AGTCGGTGCT AGGTGCCGGT 
GGGAGGCCCA ACTGCTTGGT TATCGATGAG ATTGATGGAG CCCCCACGGC GGCGATCAAC 
GTTCTCTTGA GCATCTTGAA TCGCAAGGGC CCGCAGGAGG CTGAGCAAGG GGGCACAGCT 
GTGGCTGCGG CAGGGGGACG GCGACGCAGG GCTGAGGGGG GCCTCCTGAC CAGGCCTATC 
ATCTGCATCT GCAATGATCA GTTCACACCT TCTTTGAGGC AGCTGAAGCA GCAGGCTCTT 
CTGCTCCACG TCCCACCAAC TCTGCCCTCC AGGCTGGTGC AGCGGCTCCA GGAGATCTCC 
CTGCAGCACG GCATGCGGTC TGACCCGGGT GCACTGGCTG CCCTCTGTGA GAAGACAGAC 
AATGACATCC GTGCCTGCAT CAACACACTA CAGTTTCTGT ATGGGAGGGG CCAGCGGGAG 
CTGAGCGTGA AGGATGTGCA GACCACCCAT GTCGGCCTGA AAGACCAGCG CAAGGGGCTA 
TTCTCTGTGT GGCAAGAGGT CTTCCAGTTG CCCAGGGCTC AAAGGCGACT TGTGGGCCAG 
GACCTGATGC TACCAACCCA TGCTCTCCTG CTCAATGACA GTGACAAGGG CTCTCTGACC 
TTGGCTTCCC AGCGCTTCTA CCACATCCTG CGTGTGACCA CTTCTGCAGG CGAGCATGAG 
AAGGTTGTGC AGGGCCTGTT TGACAACTTT CTACGCCTGC GGCTTCGGGA CTCCAGCCTG 
GGCACCGTGT GCTGTGCCCT TGACTGGCTG GCCTTCGATG ACCTGCTGGA ACAGGCGGCC 
CACCACGGCC AGAGCTTTCA GCTGCTGCGT TACTTGCCCT TCCTGCCCGC GGCCTTCCAC 
GTGCTCTTCG CCTCCAGCCA CGTGCCGCGC ATCACTTTCC CCAGCAGCCA GCAGGAGGCC 
CAGACCCGGT TGAGCCAGAC AAGGAACCAC ATACAGACTC TGGTGTCAGG AATGGCACCA 
GCCACTCGCA GCCGGGCCAC CCCGCAGGCT CTTGTTCTAG ATACTCTCTG CCTGCTCCTG 
GATGTCCTCT CGCCTAAGCT GCGCCCCGTG AGCACACAGC TGTACAGTGC CCGTGAGAAG 
CAGCAGTTGT CGAGCCTTGT GGGCACCATG CTTGCCTATA GCCTCACCTA CCACCAGGAG 
CGCACACCTG ATGGGCAGTA CCTCTACAGG CTGGAGCCGA ATGTGGAGGA GGTCTGCCGC 
TTCCCAGAAC TACCTGCCCG CAAGCCCCTC ACCTACCAGG CTAAGCAGCT TATTGCCCGG 
GAGATTGAAA TGGAGAAGAT GTGGAGGGCA GAGGCTTTGG CCTGGACTGG AGGTGGCCCT 
CAGGTGGACC AGGGTCCCTC AGGGCCTGCA AATCTGCGGA CGGACAGTGG GGAAAAGGGG 
ACGAGGCAGC CTGCCCCCCG TAACCATGAG CAGCGGCTAG AACACATCAT GAAGAGAGCA 
GCCGTGCAGG AGCAGCCTGA GAGGGACTTC TTTGGACGTG TGGTCATCAG GAAAGTGGCA 
GTCCCAAGCA AAGAGGTTGA GGCCCCCCAG AAGGACACGG ACGAGTGGCG CATGGGTGTG 
GCAGTGGGCA GGAGTGAGGT GTGGTTCCGG TTCAACGAGG GTGTCTCCAA TGCTGTTCGG 
CGCAGCCTGT ACATCAGGGA CCTGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245998.1
CDS	102..3011
Translation	MEDYEEDLYGVEDDFQNQFAAELEVLAELEAGTGDLAPSETLQTPASRPPLTFEEAIAGGDIVPRPCPAGSSGNDSRNTRKNVRRDQPAPSSPMVKRPRLDVVKRLNFEPDKEELLYPDSPPGDITPPPSPEVFPEMWDAGPSDAGADNGLMQALPSPRNPVLRRPPVLEDYINVTSTCGERAFLVLRADLTGTGVQNHLPDVQWRGRGQLDLLGVPFTSLKEQVDNKRRQQLLEEAQQLSDTLHSLRSEGEEAMLEGPPEEEPGPGQETAQHCLWVDEFAPQHYTELLSDDFTNRCLLKWLKLWDLVVFGHERPARKPRPGVEPTRVGKEAPAPGKWKSHEQVLEEMLEAELDPSRRPRQKVALLCGPPGLGKTTLAHVVARHAGYCVVEMNASDDRSPEAFRTRIEAATQMESVLGAGGRPNCLVIDEIDGAPTAAINVLLSILNRKGPQEAEQGGTAVAAAGGRRRRAEGGLLTRPIICICNDQFTPSLRQLKQQALLLHVPPTLPSRLVQRLQEISLQHGMRSDPGALAALCEKTDNDIRACINTLQFLYGRGQRELSVKDVQTTHVGLKDQRKGLFSVWQEVFQLPRAQRRLVGQDLMLPTHALLLNDSDKGSLTLASQRFYHILRVTTSAGEHEKVVQGLFDNFLRLRLRDSSLGTVCCALDWLAFDDLLEQAAHHGQSFQLLRYLPFLPAAFHVLFASSHVPRITFPSSQQEAQTRLSQTRNHIQTLVSGMAPATRSRATPQALVLDTLCLLLDVLSPKLRPVSTQLYSAREKQQLSSLVGTMLAYSLTYHQERTPDGQYLYRLEPNVEEVCRFPELPARKPLTYQAKQLIAREIEMEKMWRAEALAWTGGGPQVDQGPSGPANLRTDSGEKGTRQPAPRNHEQRLEHIMKRAAVQEQPERDFFGRVVIRKVAVPSKEVEAPQKDTDEWRMGVAVGRSEVWFRFNEGVSNAVRRSLYIRDLL

Target ORF information:

RefSeq Version	XM_006245936.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chromosome transmission fidelity factor 18 (Chtf18), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245936.3

ORF Insert Sequence:

ATGGAAGACT ACGAGGAAGA TCTGTATGGT GTGGAGGATG ACTTCCAAAA TCAGTTCGCG 
GCCGAACTCG AAGTGCTGGC GGAGCTGGAA GCAGGAACAG GGGACCTGGC GCCTTCTGAG 
ACCCTCCAGA CTCCGGCGAG CCGCCCCCCG CTGACCTTCG AAGAGGCCAT CGCTGGTGGG 
GACATTGTCC CACGTCCTTG CCCTGCAGGG TCCTCAGGAA ATGACAGCCG CAATACCAGA 
AAGAATGTTC GTAGGGACCA GCCCGCGCCC TCCAGCCCCA TGGTCAAACG GCCCAGGTTG 
GACGTGGTGA AGAGGCTGAA CTTCGAGCCA GATAAAGAGG AGCTCTTGTA CCCCGATTCT 
CCTCCTGGGG ACATCACCCC CCCACCGAGT CCAGAGGTCT TCCCTGAGAT GTGGGATGCT 
GGGCCCTCAG ATGCTGGTGC TGACAATGGC CTCATGCAGG CATTGCCAAG TCCCCGCAAT 
CCCGTTCTGA GACGGCCCCC TGTCTTAGAG GATTACATCA ATGTGACGTC CACGTGTGGA 
GAGCGGGCCT TTTTGGTGCT TCGGGCTGAC CTCACAGGCA CTGGGGTGCA GAACCATCTT 
CCTGATGTCC AGTGGCGAGG CCGTGGCCAG CTGGACCTTC TGGGTGTCCC CTTCACCTCC 
CTGAAGGAGC AAGTTGACAA CAAGAGACGG CAGCAACTGC TTGAGGAGGC CCAACAGCTT 
TCAGACACTC TACATAGCCT CAGGTCTGAA GGGGAAGAGG CCATGCTTGA GGGGCCCCCT 
GAGGAGGAGC CGGGCCCTGG CCAGGAGACT GCTCAGCACT GCCTCTGGGT GGACGAGTTT 
GCACCCCAGC ACTACACAGA ACTGCTCAGT GATGACTTTA CCAACCGCTG CCTTCTCAAA 
TGGCTGAAGC TATGGGACCT TGTGGTGTTT GGTCATGAGA GGCCTGCCCG GAAGCCCAGA 
CCTGGTGTAG AGCCCACCCG GGTTGGGAAG GAGGCCCCAG CCCCTGGCAA GTGGAAGAGT 
CATGAGCAGG TACTGGAGGA AATGCTAGAA GCTGAGCTGG ACCCGAGCCG GAGGCCTCGG 
CAGAAGGTGG CACTGCTGTG TGGCCCTCCA GGATTAGGCA AGACCACGCT GGCTCATGTG 
GTCGCAAGGC ATGCTGGGTA TTGCGTGGTA GAGATGAATG CAAGTGATGA CCGTAGCCCC 
GAGGCCTTCC GTACACGCAT TGAAGCAGCT ACACAAATGG AGTCGGTGCT AGGTGCCGGT 
GGGAGGCCCA ACTGCTTGGT TATCGATGAG ATTGATGGAG CCCCCACGGC GGCGATCAAC 
GTTCTCTTGA GCATCTTGAA TCGCAAGGGC CCGCAGGAGG CTGAGCAAGG GGGCACAGCT 
GTGGCTGCGG CAGGGGGACG GCGACGCAGG GCTGAGGGGG GCCTCCTGAC CAGGCCTATC 
ATCTGCATCT GCAATGATCA GTTCACACCT TCTTTGAGGC AGCTGAAGCA GCAGGCTCTT 
CTGCTCCACG TCCCACCAAC TCTGCCCTCC AGGCTGGTGC AGCGGCTCCA GGAGATCTCC 
CTGCAGCACG GCATGCGGTC TGACCCGGGT GCACTGGCTG CCCTCTGTGA GAAGACAGAC 
AATGACATCC GTGCCTGCAT CAACACACTA CAGTTTCTGT ATGGGAGGGG CCAGCGGGAG 
CTGAGCGTGA AGGATGTGCA GACCACCCAT GTCGGCCTGA AAGACCAGCG CAAGGGGCTA 
TTCTCTGTGT GGCAAGAGGT CTTCCAGTTG CCCAGGGCTC AAAGGCGACT TGTGGGCCAG 
GACCTGATGC TACCAACCCA TGCTCTCCTG CTCAATGACA GTGACAAGGG CTCTCTGACC 
TTGGCTTCCC AGCGCTTCTA CCACATCCTG CGTGTGACCA CTTCTGCAGG CGAGCATGAG 
AAGGTTGTGC AGGGCCTGTT TGACAACTTT CTACGCCTGC GGCTTCGGGA CTCCAGCCTG 
GGCACCGTGT GCTGTGCCCT TGACTGGCTG GCCTTCGATG ACCTGCTGGA ACAGGCGGCC 
CACCACGGCC AGAGCTTTCA GCTGCTGCGT TACTTGCCCT TCCTGCCCGC GGCCTTCCAC 
GTGCTCTTCG CCTCCAGCCA CGTGCCGCGC ATCACTTTCC CCAGCAGCCA GCAGGAGGCC 
CAGACCCGGT TGAGCCAGAC AAGGAACCAC ATACAGACTC TGGTGTCAGG AATGGCACCA 
GCCACTCGCA GCCGGGCCAC CCCGCAGGCT CTTGTTCTAG ATACTCTCTG CCTGCTCCTG 
GATGTCCTCT CGCCTAAGCT GCGCCCCGTG AGCACACAGC TGTACAGTGC CCGTGAGAAG 
CAGCAGTTGT CGAGCCTTGT GGGCACCATG CTTGCCTATA GCCTCACCTA CCACCAGGAG 
CGCACACCTG ATGGGCAGTA CCTCTACAGG CTGGAGCCGA ATGTGGAGGA GGTCTGCCGC 
TTCCCAGAAC TACCTGCCCG CAAGCCCCTC ACCTACCAGG CTAAGCAGCT TATTGCCCGG 
GAGATTGAAA TGGAGAAGAT GTGGAGGGCA GAGGCTTTGG CCTGGACTGG AGGTGGCCCT 
CAGGTGGACC AGGGTCCCTC AGGGCCTGCA AATCTGCGGA CGGACAGTGG GGAAAAGGGG 
ACGAGGCAGC CTGCCCCCCG TAACCATGAG CAGCGGCTAG AACACATCAT GAAGAGAGCA 
GCCGTGCAGG AGCAGCCTGA GAGGGACTTC TTTGGACGTG TGGTCATCAG GAAAGTGGCA 
GTCCCAAGCA AAGAGGTTGA GGCCCCCCAG AAGGACACGG ACGAGTGGCG CATGGGTGTG 
GCAGTGGGCA GGAGTGAGGT GTGGTTCCGG TTCAACGAGG GTGTCTCCAA TGCTGTTCGG 
CGCAGCCTGT ACATCAGGGA CCTGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chtf18 ( XM_017597066.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452555.1 Rattus norvegicus chromosome transmission fidelity factor 18 (Chtf18), transcript variant X2, mRNA.

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CloneID	ORa57208
Clone ID Related Accession (Same CDS sequence)	XM_017597066.1
Accession Version	XM_017597066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	chromosome transmission fidelity protein 18 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACT ACGAGGAAGA TCTGTATGGT GTGGAGGATG ACTTCCAAAA TCAGTTCGCG 
GCCGAACTCG AAGTGCTGGC GGAGCTGGAA GCAGGAACAG GGGACCTGGC GCCTTCTGAG 
ACCCTCCAGA CTCCGGCGAG CCGCCCCCCG CTGACCTTCG AAGAGGCCAT CGCTGGTGGG 
GACATTGTCC CACGTCCTTG CCCTGCAGGG TCCTCAGGAA ATGACAGCCG CAATACCAGA 
AAGAATGTTC GTAGGGACCA GCCCGCGCCC TCCAGCCCCA TGGTCAAACG GCCCAGGTTG 
GACGTGGTGA AGAGGCTGAA CTTCGAGCCA GATAAAGAGG AGCTCTTGTA CCCCGATTCT 
CCTCCTGGGG ACATCACCCC CCCACCGAGT CCAGAGGTCT TCCCTGAGAT GTGGGATGCT 
GGGCCCTCAG ATGCTGGTGC TGACAATGGC CTCATGCAGG CATTGCCAAG TCCCCGCAAT 
CCCGTTCTGA GACGGCCCCC TGTCTTAGAG GATTACATCA ATGTGACGTC CACGTGTGGA 
GAGCGGGCCT TTTTGGTGCT TCGGGCTGAC CTCACAGGCA CTGGGGTGCA GAACCATCTT 
CCTGATGTCC AGTGGCGAGG CCGTGGCCAG CTGGACCTTC TGGGTGTCCC CTTCACCTCC 
CTGAAGGAGC AAGTTGACAA CAAGAGACGG CAGCAACTGC TTGAGGAGGC CCAACAGCTT 
TCAGACACTC TACATAGCCT CAGGTCTGAA GGGGAAGAGG CCATGCTTGA GGGGCCCCCT 
GAGGAGGAGC CGGGCCCTGG CCAGGAGACT GCTCAGCACT GCCTCTGGGT GGACGAGTTT 
GCACCCCAGC ACTACACAGA ACTGCTCAGT GATGACTTTA CCAACCGCTG CCTTCTCAAA 
TGGCTGAAGC TATGGGACCT TGTGGTGTTT GGTCATGAGA GGCCTGCCCG GAAGCCCAGA 
CCTGGTGTAG AGCCCACCCG GGTTGGGAAG GAGGCCCCAG CCCCTGGCAA GTGGAAGAGT 
CATGAGCAGG TACTGGAGGA AATGCTAGAA GCTGAGCTGG ACCCGAGCCG GAGGCCTCGG 
CAGAAGGTGG CACTGCTGTG TGGCCCTCCA GGATTAGGCA AGACCACGCT GGCTCATGTG 
GTCGCAAGGC ATGCTGGGTA TTGCGTGGTA GAGATGAATG CAAGTGATGA CCGTAGCCCC 
GAGGCCTTCC GTACACGCAT TGAAGCAGCT ACACAAATGG AGTCGGTGCT AGGTGCCGGT 
GGGAGGCCCA ACTGCTTGGT TATCGATGAG ATTGATGGAG CCCCCACGGC GGCGATCAAC 
GTTCTCTTGA GCATCTTGAA TCGCAAGGGC CCGCAGGAGG CTGAGCAAGG GGGCACAGCT 
GTGGCTGCGG CAGGGGGACG GCGACGCAGG GCTGAGGGGG GCCTCCTGAC CAGGCCTATC 
ATCTGCATCT GCAATGATCA GTTCACACCT TCTTTGAGGC AGCTGAAGCA GCAGGCTCTT 
CTGCTCCACG TCCCACCAAC TCTGCCCTCC AGGCTGGTGC AGCGGCTCCA GGAGATCTCC 
CTGCAGCACG GCATGCGGTC TGACCCGGGT GCACTGGCTG CCCTCTGTGA GAAGACAGAC 
AATGACATCC GTGCCTGCAT CAACACACTA CAGTTTCTGT ATGGGAGGGG CCAGCGGGAG 
CTGAGCGTGA AGGATGTGCA GACCACCCAT GTCGGCCTGA AAGACCAGCG CAAGGGGCTA 
TTCTCTGTGT GGCAAGAGGT CTTCCAGTTG CCCAGGGCTC AAAGGCGACT TGTGGGCCAG 
GACCTGATGC TACCAACCCA TGCTCTCCTG CTCAATGACA GTGACAAGGG CTCTCTGACC 
TTGGCTTCCC AGCGCTTCTA CCACATCCTG CGTGTGACCA CTTCTGCAGG CGAGCATGAG 
AAGGTTGTGC AGGGCCTGTT TGACAACTTT CTACGCCTGC GGCTTCGGGA CTCCAGCCTG 
GGCACCGTGT GCTGTGCCCT TGACTGGCTG GCCTTCGATG ACCTGCTGGA ACAGGCGGCC 
CACCACGGCC AGAGCTTTCA GCTGCTGCGT TACTTGCCCT TCCTGCCCGC GGCCTTCCAC 
GTGCTCTTCG CCTCCAGCCA CGTGCCGCGC ATCACTTTCC CCAGCAGCCA GCAGGAGGCC 
CAGACCCGGT TGAGCCAGAC AAGGAACCAC ATACAGACTC TGGTGTCAGG AATGGCACCA 
GCCACTCGCA GCCGGGCCAC CCCGCAGGCT CTTGTTCTAG ATACTCTCTG CCTGCTCCTG 
GATGTCCTCT CGCCTAAGCT GCGCCCCGTG AGCACACAGC TGTACAGTGC CCGTGAGAAG 
CAGCAGTTGT CGAGCCTTGT GGGCACCATG CTTGCCTATA GCCTCACCTA CCACCAGGAG 
CGCACACCTG ATGGGCAGTA CCTCTACAGG CTGGAGCCGA ATGTGGAGGA GGTCTGCCGC 
TTCCCAGAAC TACCTGCCCG CAAGCCCCTC ACCTACCAGG CTAAGCAGCT TATTGCCCGG 
GAGATTGAAA TGGAGAAGAT GTGGAGGGCA GAGGCTTTGG CCTGGACTGG AGGTGGCCCT 
CAGGTGGACC AGGGTCCCTC AGGGCCTGCA AATCTGCGGA CGGACAGTGG GGAAAAGGGG 
ACGAGGCAGC CTGCCCCCCG TAACCATGAG CAGCGGCTAG AACACATCAT GAAGAGAGCA 
GCCGTGCAGG AGCAGGCAAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452555.1
CDS	103..2826
Translation	MEDYEEDLYGVEDDFQNQFAAELEVLAELEAGTGDLAPSETLQTPASRPPLTFEEAIAGGDIVPRPCPAGSSGNDSRNTRKNVRRDQPAPSSPMVKRPRLDVVKRLNFEPDKEELLYPDSPPGDITPPPSPEVFPEMWDAGPSDAGADNGLMQALPSPRNPVLRRPPVLEDYINVTSTCGERAFLVLRADLTGTGVQNHLPDVQWRGRGQLDLLGVPFTSLKEQVDNKRRQQLLEEAQQLSDTLHSLRSEGEEAMLEGPPEEEPGPGQETAQHCLWVDEFAPQHYTELLSDDFTNRCLLKWLKLWDLVVFGHERPARKPRPGVEPTRVGKEAPAPGKWKSHEQVLEEMLEAELDPSRRPRQKVALLCGPPGLGKTTLAHVVARHAGYCVVEMNASDDRSPEAFRTRIEAATQMESVLGAGGRPNCLVIDEIDGAPTAAINVLLSILNRKGPQEAEQGGTAVAAAGGRRRRAEGGLLTRPIICICNDQFTPSLRQLKQQALLLHVPPTLPSRLVQRLQEISLQHGMRSDPGALAALCEKTDNDIRACINTLQFLYGRGQRELSVKDVQTTHVGLKDQRKGLFSVWQEVFQLPRAQRRLVGQDLMLPTHALLLNDSDKGSLTLASQRFYHILRVTTSAGEHEKVVQGLFDNFLRLRLRDSSLGTVCCALDWLAFDDLLEQAAHHGQSFQLLRYLPFLPAAFHVLFASSHVPRITFPSSQQEAQTRLSQTRNHIQTLVSGMAPATRSRATPQALVLDTLCLLLDVLSPKLRPVSTQLYSAREKQQLSSLVGTMLAYSLTYHQERTPDGQYLYRLEPNVEEVCRFPELPARKPLTYQAKQLIAREIEMEKMWRAEALAWTGGGPQVDQGPSGPANLRTDSGEKGTRQPAPRNHEQRLEHIMKRAAVQEQAT

Target ORF information:

RefSeq Version	XM_017597066.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chromosome transmission fidelity factor 18 (Chtf18), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597066.1

ORF Insert Sequence:

ATGGAAGACT ACGAGGAAGA TCTGTATGGT GTGGAGGATG ACTTCCAAAA TCAGTTCGCG 
GCCGAACTCG AAGTGCTGGC GGAGCTGGAA GCAGGAACAG GGGACCTGGC GCCTTCTGAG 
ACCCTCCAGA CTCCGGCGAG CCGCCCCCCG CTGACCTTCG AAGAGGCCAT CGCTGGTGGG 
GACATTGTCC CACGTCCTTG CCCTGCAGGG TCCTCAGGAA ATGACAGCCG CAATACCAGA 
AAGAATGTTC GTAGGGACCA GCCCGCGCCC TCCAGCCCCA TGGTCAAACG GCCCAGGTTG 
GACGTGGTGA AGAGGCTGAA CTTCGAGCCA GATAAAGAGG AGCTCTTGTA CCCCGATTCT 
CCTCCTGGGG ACATCACCCC CCCACCGAGT CCAGAGGTCT TCCCTGAGAT GTGGGATGCT 
GGGCCCTCAG ATGCTGGTGC TGACAATGGC CTCATGCAGG CATTGCCAAG TCCCCGCAAT 
CCCGTTCTGA GACGGCCCCC TGTCTTAGAG GATTACATCA ATGTGACGTC CACGTGTGGA 
GAGCGGGCCT TTTTGGTGCT TCGGGCTGAC CTCACAGGCA CTGGGGTGCA GAACCATCTT 
CCTGATGTCC AGTGGCGAGG CCGTGGCCAG CTGGACCTTC TGGGTGTCCC CTTCACCTCC 
CTGAAGGAGC AAGTTGACAA CAAGAGACGG CAGCAACTGC TTGAGGAGGC CCAACAGCTT 
TCAGACACTC TACATAGCCT CAGGTCTGAA GGGGAAGAGG CCATGCTTGA GGGGCCCCCT 
GAGGAGGAGC CGGGCCCTGG CCAGGAGACT GCTCAGCACT GCCTCTGGGT GGACGAGTTT 
GCACCCCAGC ACTACACAGA ACTGCTCAGT GATGACTTTA CCAACCGCTG CCTTCTCAAA 
TGGCTGAAGC TATGGGACCT TGTGGTGTTT GGTCATGAGA GGCCTGCCCG GAAGCCCAGA 
CCTGGTGTAG AGCCCACCCG GGTTGGGAAG GAGGCCCCAG CCCCTGGCAA GTGGAAGAGT 
CATGAGCAGG TACTGGAGGA AATGCTAGAA GCTGAGCTGG ACCCGAGCCG GAGGCCTCGG 
CAGAAGGTGG CACTGCTGTG TGGCCCTCCA GGATTAGGCA AGACCACGCT GGCTCATGTG 
GTCGCAAGGC ATGCTGGGTA TTGCGTGGTA GAGATGAATG CAAGTGATGA CCGTAGCCCC 
GAGGCCTTCC GTACACGCAT TGAAGCAGCT ACACAAATGG AGTCGGTGCT AGGTGCCGGT 
GGGAGGCCCA ACTGCTTGGT TATCGATGAG ATTGATGGAG CCCCCACGGC GGCGATCAAC 
GTTCTCTTGA GCATCTTGAA TCGCAAGGGC CCGCAGGAGG CTGAGCAAGG GGGCACAGCT 
GTGGCTGCGG CAGGGGGACG GCGACGCAGG GCTGAGGGGG GCCTCCTGAC CAGGCCTATC 
ATCTGCATCT GCAATGATCA GTTCACACCT TCTTTGAGGC AGCTGAAGCA GCAGGCTCTT 
CTGCTCCACG TCCCACCAAC TCTGCCCTCC AGGCTGGTGC AGCGGCTCCA GGAGATCTCC 
CTGCAGCACG GCATGCGGTC TGACCCGGGT GCACTGGCTG CCCTCTGTGA GAAGACAGAC 
AATGACATCC GTGCCTGCAT CAACACACTA CAGTTTCTGT ATGGGAGGGG CCAGCGGGAG 
CTGAGCGTGA AGGATGTGCA GACCACCCAT GTCGGCCTGA AAGACCAGCG CAAGGGGCTA 
TTCTCTGTGT GGCAAGAGGT CTTCCAGTTG CCCAGGGCTC AAAGGCGACT TGTGGGCCAG 
GACCTGATGC TACCAACCCA TGCTCTCCTG CTCAATGACA GTGACAAGGG CTCTCTGACC 
TTGGCTTCCC AGCGCTTCTA CCACATCCTG CGTGTGACCA CTTCTGCAGG CGAGCATGAG 
AAGGTTGTGC AGGGCCTGTT TGACAACTTT CTACGCCTGC GGCTTCGGGA CTCCAGCCTG 
GGCACCGTGT GCTGTGCCCT TGACTGGCTG GCCTTCGATG ACCTGCTGGA ACAGGCGGCC 
CACCACGGCC AGAGCTTTCA GCTGCTGCGT TACTTGCCCT TCCTGCCCGC GGCCTTCCAC 
GTGCTCTTCG CCTCCAGCCA CGTGCCGCGC ATCACTTTCC CCAGCAGCCA GCAGGAGGCC 
CAGACCCGGT TGAGCCAGAC AAGGAACCAC ATACAGACTC TGGTGTCAGG AATGGCACCA 
GCCACTCGCA GCCGGGCCAC CCCGCAGGCT CTTGTTCTAG ATACTCTCTG CCTGCTCCTG 
GATGTCCTCT CGCCTAAGCT GCGCCCCGTG AGCACACAGC TGTACAGTGC CCGTGAGAAG 
CAGCAGTTGT CGAGCCTTGT GGGCACCATG CTTGCCTATA GCCTCACCTA CCACCAGGAG 
CGCACACCTG ATGGGCAGTA CCTCTACAGG CTGGAGCCGA ATGTGGAGGA GGTCTGCCGC 
TTCCCAGAAC TACCTGCCCG CAAGCCCCTC ACCTACCAGG CTAAGCAGCT TATTGCCCGG 
GAGATTGAAA TGGAGAAGAT GTGGAGGGCA GAGGCTTTGG CCTGGACTGG AGGTGGCCCT 
CAGGTGGACC AGGGTCCCTC AGGGCCTGCA AATCTGCGGA CGGACAGTGG GGAAAAGGGG 
ACGAGGCAGC CTGCCCCCCG TAACCATGAG CAGCGGCTAG AACACATCAT GAAGAGAGCA 
GCCGTGCAGG AGCAGGCAAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Disruption of CHTF18 causes defective meiotic recombination in male mice.
PLoS genetics8(11)e1002996(2012)
Berkowitz KM,Sowash AR,Koenig LR,Urcuyo D,Khan F,Yang F,Wang PJ,Jongens TA,Kaestner KH

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Germline expression of mammalian CTF18, an evolutionarily conserved protein required for germ cell proliferation in the fly and sister chromatid cohesion in yeast.
Molecular human reproduction14(3)143-50(2008 Mar)
Berkowitz KM,Kaestner KH,Jongens TA

The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA.
Proceedings of the National Academy of Sciences of the United States of America100(18)10237-42(2003 Sep)
Bermudez VP,Maniwa Y,Tappin I,Ozato K,Yokomori K,Hurwitz J

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Chtf18 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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