Pcyt1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcyt1b
Entrez Gene ID	286936
Full Name	phosphate cytidylyltransferase 1, choline, beta
Synonyms	CCT-beta,CTB,Cctbeta
General protein information	Preferred Names phosphate cytidylyltransferase 1, choline, beta Names choline-phosphate cytidylyltransferase B CCT B CT B CTP:phosphocholine cytidylyltransferase B choline phosphate cytidylyltransferase 1 beta phosphate cytidylyltransferase 1, choline, beta isoform phosphorylcholine transferase B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq22

mRNA and Protein(s)

mRNA	Protein	Name
XM_008773254.2	XP_008771476.1	choline-phosphate cytidylyltransferase B isoform X3
XM_006257016.3	XP_006257078.1	choline-phosphate cytidylyltransferase B isoform X1
NM_173151.1	NP_775174.1	choline-phosphate cytidylyltransferase B
XM_006257017.3	XP_006257079.1	choline-phosphate cytidylyltransferase B isoform X2

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os10g0578900	NP_001065503.1
Bos taurus (cattle)	PCYT1B	NP_001179980.1
Xenopus tropicalis (tropical clawed frog)	pcyt1b	XP_004911814.1
Homo sapiens (human)	PCYT1B	NP_004836.2
Canis lupus familiaris (dog)	PCYT1B	XP_548900.2
Rattus norvegicus (Norway rat)	Pcyt1b	NP_775174.1
Danio rerio (zebrafish)	pcyt1ba	NP_001076415.1
Oryza sativa (rice)	Os08g0161800	NP_001061052.1
Mus musculus (house mouse)	Pcyt1b	NP_997593.1
Macaca mulatta (Rhesus monkey)	PCYT1B	XP_001090055.2
Gallus gallus (chicken)	PCYT1B	XP_416793.4

Gene Ontology
Bibliography

Related articles in PubMed

Functions of membrane binding domain of CTP:phosphocholine cytidylyltransferase in alveolar type II cells.
Ridsdale R, Tseu I, Wang J, Post M
American journal of respiratory cell and molecular biology43(1)74-87(2010 Jul)

Crystal structure of a mammalian CTP: phosphocholine cytidylyltransferase catalytic domain reveals novel active site residues within a highly conserved nucleotidyltransferase fold.
Lee J, Johnson J, Ding Z, Paetzel M, Cornell RB
The Journal of biological chemistry284(48)33535-48(2009 Nov)

Phosphatidylcholine biosynthesis via CTP:phosphocholine cytidylyltransferase 2 facilitates neurite outgrowth and branching.
Carter JM, Demizieux L, Campenot RB, Vance DE, Vance JE
The Journal of biological chemistry283(1)202-12(2008 Jan)

Disruption of CCTbeta2 expression leads to gonadal dysfunction.
Jackowski S, Rehg JE, Zhang YM, Wang J, Miller K, Jackson P, Karim MA
Molecular and cellular biology24(11)4720-33(2004 Jun)

Gene structure, expression and identification of a new CTP:phosphocholine cytidylyltransferase beta isoform.
Karim M, Jackson P, Jackowski S
Biochimica et biophysica acta1633(1)1-12(2003 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pcyt1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcyt1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03574	NM_173151.1 Latest version!	Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa39772	XM_006257016.3 Latest version!	Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa39773	XM_006257017.3 Latest version!	Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa44551	XM_008773254.2 Latest version!	Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcyt1b ( NM_173151.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_775174.1 Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), mRNA.

Price & Availability↑

CloneID	ORa03574
Clone ID Related Accession (Same CDS sequence)	NM_173151.1
Accession Version	NM_173151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Rattus norvegicus(Norway rat)
Product	choline-phosphate cytidylyltransferase B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF190256.1. ##Evidence-Data-START## Transcript exon combination :: AF190256.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCAGTAG TTACCACTGA TGCTGAGTCA GAAACAGGTA TCCCAAAATC CCTTTCCAAT 
GAGCCTCCCT CAGAAACCAT GGAGGAAATA GAGCACACAT GCCCACAGCC TCGACTGACC 
CTGACTGCAC CAGCCCCATT TGCCGATGAG TCCAGCTGCC AGTGCCAAGC GCCTCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACTCCAGTTG ACAGGCCTGT TAGAGTATAT 
GCAGACGGAA TATTCGACCT CTTCCACTCA GGTCATGCAA GGGCACTTAT GCAAGCAAAA 
ACCCTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGACCT AACTCACAAA 
TTCAAAGGTT TTACTGTGAT GAATGAGGCA GAGAGATATG AAGCTCTCAG ACACTGTCGC 
TACGTGGACG AAGTCATCAG AGATGCACCA TGGACTCTTA CTCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCACGATGAC ATTCCGTACT CATCTGCCGG TTCTGATGAC 
GTTTATAAGC ATATCAAGGA AGCAGGCATG TTTGTTCCAA CACAAAGAAC AGAAGGCATC 
TCAACATCAG ACATCATCAC CAGAATTGTC CGTGACTATG ACGTATATGC CCGGCGTAAT 
CTCCAGAGAG GGTACACAGC CAAGGAGCTG AATGTCAGCT TTATAAACGA GAAGAAGTAC 
CGTTTCCAGA ACCAGGTGGA CAAGATGAAA GAAAAGGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TTAACAGAGT AGAAGAAAAG AGTCACGACC TAATTCAAAA GTGGGAAGAA 
AAATCAAGAG AATTCATTGG CAACTTCCTA GAATTGTTTG GGCCAGATGG TGCATGGAAG 
CAGATGTTCC AGGAGAGGAG TAGCCGGATG CTACAGGCCT TATCTCCAAA GCAGAGTCCT 
GTGAGCAGCC CGACCCGGAG CCGGTCCCCT TCCCGCTCTC CATCTCCTAC CTTCTCCTGG 
CTCCCAAACA AGACCTCTCC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC CATCAGCAGC 
ATGAGTGAGG GGGATGAGGA TGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775174.1
CDS	11..1120
Translation	MPVVTTDAESETGIPKSLSNEPPSETMEEIEHTCPQPRLTLTAPAPFADESSCQCQAPHEKLTIAQARLGTPVDRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNEAERYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPYSSAGSDDVYKHIKEAGMFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKKYRFQNQVDKMKEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELFGPDGAWKQMFQERSSRMLQALSPKQSPVSSPTRSRSPSRSPSPTFSWLPNKTSPPSSPKAASASISSMSEGDEDEK

Target ORF information:

RefSeq Version	NM_173151.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173151.1

ORF Insert Sequence:

ATGCCAGTAG TTACCACTGA TGCTGAGTCA GAAACAGGTA TCCCAAAATC CCTTTCCAAT 
GAGCCTCCCT CAGAAACCAT GGAGGAAATA GAGCACACAT GCCCACAGCC TCGACTGACC 
CTGACTGCAC CAGCCCCATT TGCCGATGAG TCCAGCTGCC AGTGCCAAGC GCCTCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACTCCAGTTG ACAGGCCTGT TAGAGTATAT 
GCAGACGGAA TATTCGACCT CTTCCACTCA GGTCATGCAA GGGCACTTAT GCAAGCAAAA 
ACCCTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGACCT AACTCACAAA 
TTCAAAGGTT TTACTGTGAT GAATGAGGCA GAGAGATATG AAGCTCTCAG ACACTGTCGC 
TACGTGGACG AAGTCATCAG AGATGCACCA TGGACTCTTA CTCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCACGATGAC ATTCCGTACT CATCTGCCGG TTCTGATGAC 
GTTTATAAGC ATATCAAGGA AGCAGGCATG TTTGTTCCAA CACAAAGAAC AGAAGGCATC 
TCAACATCAG ACATCATCAC CAGAATTGTC CGTGACTATG ACGTATATGC CCGGCGTAAT 
CTCCAGAGAG GGTACACAGC CAAGGAGCTG AATGTCAGCT TTATAAACGA GAAGAAGTAC 
CGTTTCCAGA ACCAGGTGGA CAAGATGAAA GAAAAGGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TTAACAGAGT AGAAGAAAAG AGTCACGACC TAATTCAAAA GTGGGAAGAA 
AAATCAAGAG AATTCATTGG CAACTTCCTA GAATTGTTTG GGCCAGATGG TGCATGGAAG 
CAGATGTTCC AGGAGAGGAG TAGCCGGATG CTACAGGCCT TATCTCCAAA GCAGAGTCCT 
GTGAGCAGCC CGACCCGGAG CCGGTCCCCT TCCCGCTCTC CATCTCCTAC CTTCTCCTGG 
CTCCCAAACA AGACCTCTCC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC CATCAGCAGC 
ATGAGTGAGG GGGATGAGGA TGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcyt1b ( XM_006257016.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257078.1 Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39772
Clone ID Related Accession (Same CDS sequence)	XM_006257016.3
Accession Version	XM_006257016.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	choline-phosphate cytidylyltransferase B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257016.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTGCC ACGCTTCTGA ATATGAGGTT CCTTTCAGCA GAGTGTCCCG GGTGTGGACC 
CTGACTGCAC CAGCCCCATT TGCCGATGAG TCCAGCTGCC AGTGCCAAGC GCCTCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACTCCAGTTG ACAGGCCTGT TAGAGTATAT 
GCAGACGGAA TATTCGACCT CTTCCACTCA GGTCATGCAA GGGCACTTAT GCAAGCAAAA 
ACCCTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGACCT AACTCACAAA 
TTCAAAGGTT TTACTGTGAT GAATGAGGCA GAGAGATATG AAGCTCTCAG ACACTGTCGC 
TACGTGGACG AAGTCATCAG AGATGCACCA TGGACTCTTA CTCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCACGATGAC ATTCCGTACT CATCTGCCGG TTCTGATGAC 
GTTTATAAGC ATATCAAGGA AGCAGGCATG TTTGTTCCAA CACAAAGAAC AGAAGGCATC 
TCAACATCAG ACATCATCAC CAGAATTGTC CGTGACTATG ACGTATATGC CCGGCGTAAT 
CTCCAGAGAG GGTACACAGC CAAGGAGCTG AATGTCAGCT TTATAAACGA GAAGAAGTAC 
CGTTTCCAGA ACCAGGTGGA CAAGATGAAA GAAAAGGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TTAACAGAGT AGAAGAAAAG AGTCACGACC TAATTCAAAA GTGGGAAGAA 
AAATCAAGAG AATTCATTGG CAACTTCCTA GAATTGTTTG GGCCAGATGG TGCATGGAAG 
CAGATGTTCC AGGAGAGGAG TAGCCGGATG CTACAGGCCT TATCTCCAAA GCAGAGTCCT 
GTGAGCAGCC CGACCCGGAG CCGGTCCCCT TCCCGCTCTC CATCTCCTAC CTTCTCCTGG 
CTCCCAAACA AGACCTCTCC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC CATCAGCAGC 
ATGAGTGAGG GGGATGAGGA TGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257078.1
CDS	201..1250
Translation	MSCHASEYEVPFSRVSRVWTLTAPAPFADESSCQCQAPHEKLTIAQARLGTPVDRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNEAERYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPYSSAGSDDVYKHIKEAGMFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKKYRFQNQVDKMKEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELFGPDGAWKQMFQERSSRMLQALSPKQSPVSSPTRSRSPSRSPSPTFSWLPNKTSPPSSPKAASASISSMSEGDEDEK

Target ORF information:

RefSeq Version	XM_006257016.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257016.3

ORF Insert Sequence:

ATGTCGTGCC ACGCTTCTGA ATATGAGGTT CCTTTCAGCA GAGTGTCCCG GGTGTGGACC 
CTGACTGCAC CAGCCCCATT TGCCGATGAG TCCAGCTGCC AGTGCCAAGC GCCTCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACTCCAGTTG ACAGGCCTGT TAGAGTATAT 
GCAGACGGAA TATTCGACCT CTTCCACTCA GGTCATGCAA GGGCACTTAT GCAAGCAAAA 
ACCCTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGACCT AACTCACAAA 
TTCAAAGGTT TTACTGTGAT GAATGAGGCA GAGAGATATG AAGCTCTCAG ACACTGTCGC 
TACGTGGACG AAGTCATCAG AGATGCACCA TGGACTCTTA CTCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCACGATGAC ATTCCGTACT CATCTGCCGG TTCTGATGAC 
GTTTATAAGC ATATCAAGGA AGCAGGCATG TTTGTTCCAA CACAAAGAAC AGAAGGCATC 
TCAACATCAG ACATCATCAC CAGAATTGTC CGTGACTATG ACGTATATGC CCGGCGTAAT 
CTCCAGAGAG GGTACACAGC CAAGGAGCTG AATGTCAGCT TTATAAACGA GAAGAAGTAC 
CGTTTCCAGA ACCAGGTGGA CAAGATGAAA GAAAAGGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TTAACAGAGT AGAAGAAAAG AGTCACGACC TAATTCAAAA GTGGGAAGAA 
AAATCAAGAG AATTCATTGG CAACTTCCTA GAATTGTTTG GGCCAGATGG TGCATGGAAG 
CAGATGTTCC AGGAGAGGAG TAGCCGGATG CTACAGGCCT TATCTCCAAA GCAGAGTCCT 
GTGAGCAGCC CGACCCGGAG CCGGTCCCCT TCCCGCTCTC CATCTCCTAC CTTCTCCTGG 
CTCCCAAACA AGACCTCTCC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC CATCAGCAGC 
ATGAGTGAGG GGGATGAGGA TGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcyt1b ( XM_006257017.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257079.1 Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa39773
Clone ID Related Accession (Same CDS sequence)	XM_006257017.3
Accession Version	XM_006257017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	choline-phosphate cytidylyltransferase B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTAG TGGTACACCA GAAATGCACC ATGGACAAAG ACAAATTCTC ACGCAAGACC 
CTGACTGCAC CAGCCCCATT TGCCGATGAG TCCAGCTGCC AGTGCCAAGC GCCTCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACTCCAGTTG ACAGGCCTGT TAGAGTATAT 
GCAGACGGAA TATTCGACCT CTTCCACTCA GGTCATGCAA GGGCACTTAT GCAAGCAAAA 
ACCCTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGACCT AACTCACAAA 
TTCAAAGGTT TTACTGTGAT GAATGAGGCA GAGAGATATG AAGCTCTCAG ACACTGTCGC 
TACGTGGACG AAGTCATCAG AGATGCACCA TGGACTCTTA CTCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCACGATGAC ATTCCGTACT CATCTGCCGG TTCTGATGAC 
GTTTATAAGC ATATCAAGGA AGCAGGCATG TTTGTTCCAA CACAAAGAAC AGAAGGCATC 
TCAACATCAG ACATCATCAC CAGAATTGTC CGTGACTATG ACGTATATGC CCGGCGTAAT 
CTCCAGAGAG GGTACACAGC CAAGGAGCTG AATGTCAGCT TTATAAACGA GAAGAAGTAC 
CGTTTCCAGA ACCAGGTGGA CAAGATGAAA GAAAAGGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TTAACAGAGT AGAAGAAAAG AGTCACGACC TAATTCAAAA GTGGGAAGAA 
AAATCAAGAG AATTCATTGG CAACTTCCTA GAATTGTTTG GGCCAGATGG TGCATGGAAG 
CAGATGTTCC AGGAGAGGAG TAGCCGGATG CTACAGGCCT TATCTCCAAA GCAGAGTCCT 
GTGAGCAGCC CGACCCGGAG CCGGTCCCCT TCCCGCTCTC CATCTCCTAC CTTCTCCTGG 
CTCCCAAACA AGACCTCTCC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC CATCAGCAGC 
ATGAGTGAGG GGGATGAGGA TGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257079.1
CDS	365..1414
Translation	MNLVVHQKCTMDKDKFSRKTLTAPAPFADESSCQCQAPHEKLTIAQARLGTPVDRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNEAERYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPYSSAGSDDVYKHIKEAGMFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKKYRFQNQVDKMKEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELFGPDGAWKQMFQERSSRMLQALSPKQSPVSSPTRSRSPSRSPSPTFSWLPNKTSPPSSPKAASASISSMSEGDEDEK

Target ORF information:

RefSeq Version	XM_006257017.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257017.3

ORF Insert Sequence:

ATGAACCTAG TGGTACACCA GAAATGCACC ATGGACAAAG ACAAATTCTC ACGCAAGACC 
CTGACTGCAC CAGCCCCATT TGCCGATGAG TCCAGCTGCC AGTGCCAAGC GCCTCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACTCCAGTTG ACAGGCCTGT TAGAGTATAT 
GCAGACGGAA TATTCGACCT CTTCCACTCA GGTCATGCAA GGGCACTTAT GCAAGCAAAA 
ACCCTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGACCT AACTCACAAA 
TTCAAAGGTT TTACTGTGAT GAATGAGGCA GAGAGATATG AAGCTCTCAG ACACTGTCGC 
TACGTGGACG AAGTCATCAG AGATGCACCA TGGACTCTTA CTCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCACGATGAC ATTCCGTACT CATCTGCCGG TTCTGATGAC 
GTTTATAAGC ATATCAAGGA AGCAGGCATG TTTGTTCCAA CACAAAGAAC AGAAGGCATC 
TCAACATCAG ACATCATCAC CAGAATTGTC CGTGACTATG ACGTATATGC CCGGCGTAAT 
CTCCAGAGAG GGTACACAGC CAAGGAGCTG AATGTCAGCT TTATAAACGA GAAGAAGTAC 
CGTTTCCAGA ACCAGGTGGA CAAGATGAAA GAAAAGGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TTAACAGAGT AGAAGAAAAG AGTCACGACC TAATTCAAAA GTGGGAAGAA 
AAATCAAGAG AATTCATTGG CAACTTCCTA GAATTGTTTG GGCCAGATGG TGCATGGAAG 
CAGATGTTCC AGGAGAGGAG TAGCCGGATG CTACAGGCCT TATCTCCAAA GCAGAGTCCT 
GTGAGCAGCC CGACCCGGAG CCGGTCCCCT TCCCGCTCTC CATCTCCTAC CTTCTCCTGG 
CTCCCAAACA AGACCTCTCC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC CATCAGCAGC 
ATGAGTGAGG GGGATGAGGA TGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcyt1b ( XM_008773254.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771476.1 Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa44551
Clone ID Related Accession (Same CDS sequence)	XM_008773254.2
Accession Version	XM_008773254.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	choline-phosphate cytidylyltransferase B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008773254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTGA CTGCACCAGC CCCATTTGCC GATGAGTCCA GCTGCCAGTG CCAAGCGCCT 
CATGAAAAAC TGACCATTGC TCAGGCCCGC TTAGGAACTC CAGTTGACAG GCCTGTTAGA 
GTATATGCAG ACGGAATATT CGACCTCTTC CACTCAGGTC ATGCAAGGGC ACTTATGCAA 
GCAAAAACCC TGTTTCCCAA CAGCTACTTG TTGGTAGGAG TTTGCAGTGA TGACCTAACT 
CACAAATTCA AAGGTTTTAC TGTGATGAAT GAGGCAGAGA GATATGAAGC TCTCAGACAC 
TGTCGCTACG TGGACGAAGT CATCAGAGAT GCACCATGGA CTCTTACTCC AGAGTTTCTG 
GAAAAACACA AGATTGACTT TGTGGCTCAC GATGACATTC CGTACTCATC TGCCGGTTCT 
GATGACGTTT ATAAGCATAT CAAGGAAGCA GGCATGTTTG TTCCAACACA AAGAACAGAA 
GGCATCTCAA CATCAGACAT CATCACCAGA ATTGTCCGTG ACTATGACGT ATATGCCCGG 
CGTAATCTCC AGAGAGGGTA CACAGCCAAG GAGCTGAATG TCAGCTTTAT AAACGAGAAG 
AAGTACCGTT TCCAGAACCA GGTGGACAAG ATGAAAGAAA AGGTCAAGAA TGTGGAGGAA 
AGATCAAAGG AATTTGTTAA CAGAGTAGAA GAAAAGAGTC ACGACCTAAT TCAAAAGTGG 
GAAGAAAAAT CAAGAGAATT CATTGGCAAC TTCCTAGAAT TGTTTGGGCC AGATGGTGCA 
TGGAAGCAGA TGTTCCAGGA GAGGAGTAGC CGGATGCTAC AGGCCTTATC TCCAAAGCAG 
AGTCCTGTGA GCAGCCCGAC CCGGAGCCGG TCCCCTTCCC GCTCTCCATC TCCTACCTTC 
TCCTGGCTCC CAAACAAGAC CTCTCCCCCT TCCTCACCCA AAGCAGCCTC AGCCTCCATC 
AGCAGCATGA GTGAGGGGGA TGAGGATGAG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771476.1
CDS	339..1334
Translation	MTLTAPAPFADESSCQCQAPHEKLTIAQARLGTPVDRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNEAERYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPYSSAGSDDVYKHIKEAGMFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKKYRFQNQVDKMKEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELFGPDGAWKQMFQERSSRMLQALSPKQSPVSSPTRSRSPSRSPSPTFSWLPNKTSPPSSPKAASASISSMSEGDEDEK

Target ORF information:

RefSeq Version	XM_008773254.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773254.2

ORF Insert Sequence:

ATGACCCTGA CTGCACCAGC CCCATTTGCC GATGAGTCCA GCTGCCAGTG CCAAGCGCCT 
CATGAAAAAC TGACCATTGC TCAGGCCCGC TTAGGAACTC CAGTTGACAG GCCTGTTAGA 
GTATATGCAG ACGGAATATT CGACCTCTTC CACTCAGGTC ATGCAAGGGC ACTTATGCAA 
GCAAAAACCC TGTTTCCCAA CAGCTACTTG TTGGTAGGAG TTTGCAGTGA TGACCTAACT 
CACAAATTCA AAGGTTTTAC TGTGATGAAT GAGGCAGAGA GATATGAAGC TCTCAGACAC 
TGTCGCTACG TGGACGAAGT CATCAGAGAT GCACCATGGA CTCTTACTCC AGAGTTTCTG 
GAAAAACACA AGATTGACTT TGTGGCTCAC GATGACATTC CGTACTCATC TGCCGGTTCT 
GATGACGTTT ATAAGCATAT CAAGGAAGCA GGCATGTTTG TTCCAACACA AAGAACAGAA 
GGCATCTCAA CATCAGACAT CATCACCAGA ATTGTCCGTG ACTATGACGT ATATGCCCGG 
CGTAATCTCC AGAGAGGGTA CACAGCCAAG GAGCTGAATG TCAGCTTTAT AAACGAGAAG 
AAGTACCGTT TCCAGAACCA GGTGGACAAG ATGAAAGAAA AGGTCAAGAA TGTGGAGGAA 
AGATCAAAGG AATTTGTTAA CAGAGTAGAA GAAAAGAGTC ACGACCTAAT TCAAAAGTGG 
GAAGAAAAAT CAAGAGAATT CATTGGCAAC TTCCTAGAAT TGTTTGGGCC AGATGGTGCA 
TGGAAGCAGA TGTTCCAGGA GAGGAGTAGC CGGATGCTAC AGGCCTTATC TCCAAAGCAG 
AGTCCTGTGA GCAGCCCGAC CCGGAGCCGG TCCCCTTCCC GCTCTCCATC TCCTACCTTC 
TCCTGGCTCC CAAACAAGAC CTCTCCCCCT TCCTCACCCA AAGCAGCCTC AGCCTCCATC 
AGCAGCATGA GTGAGGGGGA TGAGGATGAG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functions of membrane binding domain of CTP:phosphocholine cytidylyltransferase in alveolar type II cells.
American journal of respiratory cell and molecular biology43(1)74-87(2010 Jul)
Ridsdale R,Tseu I,Wang J,Post M

Crystal structure of a mammalian CTP: phosphocholine cytidylyltransferase catalytic domain reveals novel active site residues within a highly conserved nucleotidyltransferase fold.
The Journal of biological chemistry284(48)33535-48(2009 Nov)
Lee J,Johnson J,Ding Z,Paetzel M,Cornell RB

Phosphatidylcholine biosynthesis via CTP:phosphocholine cytidylyltransferase 2 facilitates neurite outgrowth and branching.
The Journal of biological chemistry283(1)202-12(2008 Jan)
Carter JM,Demizieux L,Campenot RB,Vance DE,Vance JE

Disruption of CCTbeta2 expression leads to gonadal dysfunction.
Molecular and cellular biology24(11)4720-33(2004 Jun)
Jackowski S,Rehg JE,Zhang YM,Wang J,Miller K,Jackson P,Karim MA

Gene structure, expression and identification of a new CTP:phosphocholine cytidylyltransferase beta isoform.
Biochimica et biophysica acta1633(1)1-12(2003 Jul)
Karim M,Jackson P,Jackowski S

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Pcyt1b cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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