Rnf34 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnf34
Entrez Gene ID	282845
Full Name	ring finger protein 34
Synonyms	Momo
General protein information	Preferred Names ring finger protein 34 Names E3 ubiquitin-protein ligase RNF34 RING finger protein MOMO RING-type E3 ubiquitin transferase RNF34 ring finger protein 34, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16
Summary	has ubiquitin ligase activity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006249361.3	XP_006249423.1	E3 ubiquitin-protein ligase RNF34 isoform X2
NM_001004075.1	NP_001004075.1	E3 ubiquitin-protein ligase RNF34
XM_008769214.2	XP_008767436.1	E3 ubiquitin-protein ligase RNF34 isoform X2
XM_006249360.3	XP_006249422.1	E3 ubiquitin-protein ligase RNF34 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RNF34	XP_522545.3
Danio rerio (zebrafish)	rnf34a	NP_001025239.1
Mus musculus (house mouse)	Rnf34	NP_085041.1
Danio rerio (zebrafish)	rnf34b	NP_001103505.1
Macaca mulatta (Rhesus monkey)	RNF34	XP_001095738.1
Canis lupus familiaris (dog)	RNF34	XP_534667.2
Gallus gallus (chicken)	RNF34	NP_001006188.1
Xenopus tropicalis (tropical clawed frog)	rnf34	NP_001072224.1
Homo sapiens (human)	RNF34	NP_079402.2
Bos taurus (cattle)	RNF34	NP_001014858.1
Rattus norvegicus (Norway rat)	Rnf34	NP_001004075.1

Gene Ontology
Bibliography

Related articles in PubMed

Altered Gene Expression of RNF34 and PACAP Possibly Involved in Mechanism of Exercise-Induced Analgesia for Neuropathic Pain in Rats.
Yamaoka S, Oshima Y, Horiuchi H, Morino T, Hino M, Miura H, Ogata T
International journal of molecular sciences18(9)(2017 Sep)

Ring finger protein 34 (RNF34) interacts with and promotes γ-aminobutyric acid type-A receptor degradation via ubiquitination of the γ2 subunit.
Jin H, Chiou TT, Serwanski DR, Miralles CP, Pinal N, De Blas AL
The Journal of biological chemistry289(42)29420-36(2014 Oct)

The E3 ligase RNF34 is a novel negative regulator of the NOD1 pathway.
Zhang R, Zhao J, Song Y, Wang X, Wang L, Xu J, Song C, Liu F
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology33(6)1954-62(2014)

RNF34 is a cold-regulated E3 ubiquitin ligase for PGC-1α and modulates brown fat cell metabolism.
Wei P, Pan D, Mao C, Wang YX
Molecular and cellular biology32(2)266-75(2012 Jan)

CARPs enhance p53 turnover by degrading 14-3-3sigma and stabilizing MDM2.
Yang W, Dicker DT, Chen J, El-Deiry WS
Cell cycle (Georgetown, Tex.)7(5)670-82(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rnf34 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnf34 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa09924	NM_001004075.1 Latest version!	Rattus norvegicus ring finger protein 34 (Rnf34), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa35037	XM_006249360.3 Latest version!	Rattus norvegicus ring finger protein 34 (Rnf34), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa35038	XM_006249361.3 Latest version!	Rattus norvegicus ring finger protein 34 (Rnf34), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa35038	XM_008769214.2 Latest version!	Rattus norvegicus ring finger protein 34 (Rnf34), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnf34 ( NM_001004075.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001004075.1 Rattus norvegicus ring finger protein 34 (Rnf34), mRNA.

Price & Availability↑

CloneID	ORa09924
Clone ID Related Accession (Same CDS sequence)	NM_001004075.1
Accession Version	NM_001004075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571328000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RNF34
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC079044.1. On Sep 3, 2004 this sequence version replaced XM_341082.1. ##Evidence-Data-START## Transcript exon combination :: BC079044.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAAGGCGG GTGCTACCTC TATGTGGGCT TCATGCTGTG GACTGCTGAA TGAAGTCATG 
GGAACTGGAG CTGTCAGAGG CCAGCAGGCA GGATTCCCAG GAAGCACTGG TCCATTCAGG 
TTTACACCGA GTTCTGACTT TCCTACCTAC CCACCAGCAG CTACAGAAGG ACCCAATATA 
GTTTGCAAAG CCTGTGGACT TTCATTTTCA GTCTTTAGAA AGAAGCACGT CTGCTGTGAC 
TGCAAGAAGG ACTTTTGCTC CCTTTGTTCG GTCTCACAAG AAAACCTCCG CAGATGCTCT 
ACATGTCACC TACTCCAAGA GACAGCCTTC CAGCGCCCTC AGTTGATGAG ACTAAAAGTG 
AAGGACCTGC GGCAGTATCT GCTCCTCAGA AACGTACCGA CCGACACTTG TCGCGAGAAG 
GAGGACTTAG TGGATCTAGT GCTGTGCCAT CGTGGACTGG GCTCGGGGGA TGGCCTGGAC 
TCAAGGAGTC TCAGTTCCTC AAGATCCCAG ACTTCTAGTT TTTTTACACA ATCCTATTTT 
TCAAATTATA CACCCCCCTC TGCTACTGTG TCCTCCTTCC AGGGAGAGCT GATGGACAGA 
GAGGGCACCT TCAGGTCAGA AGTTCTCACA CAGGTACAAA GTGAATTAGC TTCAGCAAAC 
ACGGATGATG AGGATGGGGA GGAAGATGAC GACGATGATG ACGATGACGA CGACGAGGAT 
GACGATGAAC AAGAAGAAAA CTTAGAGGAA CAGAACCCGG GGCTCTCTAA GAAGAAGGCG 
AGAGCTTCCC TGTCCGACCT GTCAAGCCTT GAAGAGGTGG AAGGGATGAG CGTGCGCCAG 
CTGAAGGAAA TCCTGGCTCG GAATTTTGTC AACTATTCTG GCTGCTGTGA AAAGTGGGAG 
CTGGTGGAGA AAGTCAACCG GCTGTACAAG GAGAATGAGG AAAACCAAAA GTCATATGGT 
GAGCGGATGC AACTTCAGGA TGAGGAAGAC GACAGCCTGT GCCGAATCTG CATGGATGCG 
GTCATTGACT GTGTCCTGCT GGAGTGTGGG CACATGGTCA CCTGCACCAA GTGCGGCAAG 
CGCATGAGTG AGTGTCCCAT CTGCAGGCAG TATGTGGTTC GAGCTGTGCA TGTTTTCAAG 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004075.1
CDS	96..1241
Translation	MKAGATSMWASCCGLLNEVMGTGAVRGQQAGFPGSTGPFRFTPSSDFPTYPPAATEGPNIVCKACGLSFSVFRKKHVCCDCKKDFCSLCSVSQENLRRCSTCHLLQETAFQRPQLMRLKVKDLRQYLLLRNVPTDTCREKEDLVDLVLCHRGLGSGDGLDSRSLSSSRSQTSSFFTQSYFSNYTPPSATVSSFQGELMDREGTFRSEVLTQVQSELASANTDDEDGEEDDDDDDDDDDEDDDEQEENLEEQNPGLSKKKARASLSDLSSLEEVEGMSVRQLKEILARNFVNYSGCCEKWELVEKVNRLYKENEENQKSYGERMQLQDEEDDSLCRICMDAVIDCVLLECGHMVTCTKCGKRMSECPICRQYVVRAVHVFKS

Target ORF information:

RefSeq Version	NM_001004075.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger protein 34 (Rnf34), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004075.1

ORF Insert Sequence:

ATGAAGGCGG GTGCTACCTC TATGTGGGCT TCATGCTGTG GACTGCTGAA TGAAGTCATG 
GGAACTGGAG CTGTCAGAGG CCAGCAGGCA GGATTCCCAG GAAGCACTGG TCCATTCAGG 
TTTACACCGA GTTCTGACTT TCCTACCTAC CCACCAGCAG CTACAGAAGG ACCCAATATA 
GTTTGCAAAG CCTGTGGACT TTCATTTTCA GTCTTTAGAA AGAAGCACGT CTGCTGTGAC 
TGCAAGAAGG ACTTTTGCTC CCTTTGTTCG GTCTCACAAG AAAACCTCCG CAGATGCTCT 
ACATGTCACC TACTCCAAGA GACAGCCTTC CAGCGCCCTC AGTTGATGAG ACTAAAAGTG 
AAGGACCTGC GGCAGTATCT GCTCCTCAGA AACGTACCGA CCGACACTTG TCGCGAGAAG 
GAGGACTTAG TGGATCTAGT GCTGTGCCAT CGTGGACTGG GCTCGGGGGA TGGCCTGGAC 
TCAAGGAGTC TCAGTTCCTC AAGATCCCAG ACTTCTAGTT TTTTTACACA ATCCTATTTT 
TCAAATTATA CACCCCCCTC TGCTACTGTG TCCTCCTTCC AGGGAGAGCT GATGGACAGA 
GAGGGCACCT TCAGGTCAGA AGTTCTCACA CAGGTACAAA GTGAATTAGC TTCAGCAAAC 
ACGGATGATG AGGATGGGGA GGAAGATGAC GACGATGATG ACGATGACGA CGACGAGGAT 
GACGATGAAC AAGAAGAAAA CTTAGAGGAA CAGAACCCGG GGCTCTCTAA GAAGAAGGCG 
AGAGCTTCCC TGTCCGACCT GTCAAGCCTT GAAGAGGTGG AAGGGATGAG CGTGCGCCAG 
CTGAAGGAAA TCCTGGCTCG GAATTTTGTC AACTATTCTG GCTGCTGTGA AAAGTGGGAG 
CTGGTGGAGA AAGTCAACCG GCTGTACAAG GAGAATGAGG AAAACCAAAA GTCATATGGT 
GAGCGGATGC AACTTCAGGA TGAGGAAGAC GACAGCCTGT GCCGAATCTG CATGGATGCG 
GTCATTGACT GTGTCCTGCT GGAGTGTGGG CACATGGTCA CCTGCACCAA GTGCGGCAAG 
CGCATGAGTG AGTGTCCCAT CTGCAGGCAG TATGTGGTTC GAGCTGTGCA TGTTTTCAAG 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf34 ( XM_006249360.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249422.1 Rattus norvegicus ring finger protein 34 (Rnf34), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35037
Clone ID Related Accession (Same CDS sequence)	XM_006249360.3
Accession Version	XM_006249360.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RNF34 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAAC TGAGCTGGAC ACAAAGGAGA GGGGATGGAA GGCCAGAAAA CCGGATGCAT 
CAGCAAAGAG GCTCACAGGC GGGTGCTACC TCTATGTGGG CTTCATGCTG TGGACTGCTG 
AATGAAGTCA TGGGAACTGG AGCTGTCAGA GGCCAGCAGG CAGGATTCCC AGGAAGCACT 
GGTCCATTCA GGTTTACACC GAGTTCTGAC TTTCCTACCT ACCCACCAGC AGCTACAGAA 
GGACCCAATA TAGTTTGCAA AGCCTGTGGA CTTTCATTTT CAGTCTTTAG AAAGAAGCAC 
GTCTGCTGTG ACTGCAAGAA GGACTTTTGC TCCCTTTGTT CGGTCTCACA AGAAAACCTC 
CGCAGATGCT CTACATGTCA CCTACTCCAA GAGACAGCCT TCCAGCGCCC TCAGTTGATG 
AGACTAAAAG TGAAGGACCT GCGGCAGTAT CTGCTCCTCA GAAACGTACC GACCGACACT 
TGTCGCGAGA AGGAGGACTT AGTGGATCTA GTGCTGTGCC ATCGTGGACT GGGCTCGGGG 
GATGGCCTGG ACTCAAGGAG TCTCAGTTCC TCAAGATCCC AGACTTCTAG TTTTTTTACA 
CAATCCTATT TTTCAAATTA TACACCCCCC TCTGCTACTG TGTCCTCCTT CCAGGGAGAG 
CTGATGGACA GAGAGGGCAC CTTCAGGTCA GAAGTTCTCA CACAGGTACA AAGTGAATTA 
GCTTCAGCAA ACACGGATGA TGAGGATGGG GAGGAAGATG ACGACGATGA TGACGATGAC 
GACGACGAGG ATGACGATGA ACAAGAAGAA AACTTAGAGG AACAGAACCC GGGGCTCTCT 
AAGAAGAAGG CGAGAGCTTC CCTGTCCGAC CTGTCAAGCC TTGAAGAGGT GGAAGGGATG 
AGCGTGCGCC AGCTGAAGGA AATCCTGGCT CGGAATTTTG TCAACTATTC TGGCTGCTGT 
GAAAAGTGGG AGCTGGTGGA GAAAGTCAAC CGGCTGTACA AGGAGAATGA GGAAAACCAA 
AAGTCATATG GTGAGCGGAT GCAACTTCAG GATGAGGAAG ACGACAGCCT GTGCCGAATC 
TGCATGGATG CGGTCATTGA CTGTGTCCTG CTGGAGTGTG GGCACATGGT CACCTGCACC 
AAGTGCGGCA AGCGCATGAG TGAGTGTCCC ATCTGCAGGC AGTATGTGGT TCGAGCTGTG 
CATGTTTTCA AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249422.1
CDS	103..1320
Translation	MTKLSWTQRRGDGRPENRMHQQRGSQAGATSMWASCCGLLNEVMGTGAVRGQQAGFPGSTGPFRFTPSSDFPTYPPAATEGPNIVCKACGLSFSVFRKKHVCCDCKKDFCSLCSVSQENLRRCSTCHLLQETAFQRPQLMRLKVKDLRQYLLLRNVPTDTCREKEDLVDLVLCHRGLGSGDGLDSRSLSSSRSQTSSFFTQSYFSNYTPPSATVSSFQGELMDREGTFRSEVLTQVQSELASANTDDEDGEEDDDDDDDDDDEDDDEQEENLEEQNPGLSKKKARASLSDLSSLEEVEGMSVRQLKEILARNFVNYSGCCEKWELVEKVNRLYKENEENQKSYGERMQLQDEEDDSLCRICMDAVIDCVLLECGHMVTCTKCGKRMSECPICRQYVVRAVHVFKS

Target ORF information:

RefSeq Version	XM_006249360.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger protein 34 (Rnf34), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249360.3

ORF Insert Sequence:

ATGACCAAAC TGAGCTGGAC ACAAAGGAGA GGGGATGGAA GGCCAGAAAA CCGGATGCAT 
CAGCAAAGAG GCTCACAGGC GGGTGCTACC TCTATGTGGG CTTCATGCTG TGGACTGCTG 
AATGAAGTCA TGGGAACTGG AGCTGTCAGA GGCCAGCAGG CAGGATTCCC AGGAAGCACT 
GGTCCATTCA GGTTTACACC GAGTTCTGAC TTTCCTACCT ACCCACCAGC AGCTACAGAA 
GGACCCAATA TAGTTTGCAA AGCCTGTGGA CTTTCATTTT CAGTCTTTAG AAAGAAGCAC 
GTCTGCTGTG ACTGCAAGAA GGACTTTTGC TCCCTTTGTT CGGTCTCACA AGAAAACCTC 
CGCAGATGCT CTACATGTCA CCTACTCCAA GAGACAGCCT TCCAGCGCCC TCAGTTGATG 
AGACTAAAAG TGAAGGACCT GCGGCAGTAT CTGCTCCTCA GAAACGTACC GACCGACACT 
TGTCGCGAGA AGGAGGACTT AGTGGATCTA GTGCTGTGCC ATCGTGGACT GGGCTCGGGG 
GATGGCCTGG ACTCAAGGAG TCTCAGTTCC TCAAGATCCC AGACTTCTAG TTTTTTTACA 
CAATCCTATT TTTCAAATTA TACACCCCCC TCTGCTACTG TGTCCTCCTT CCAGGGAGAG 
CTGATGGACA GAGAGGGCAC CTTCAGGTCA GAAGTTCTCA CACAGGTACA AAGTGAATTA 
GCTTCAGCAA ACACGGATGA TGAGGATGGG GAGGAAGATG ACGACGATGA TGACGATGAC 
GACGACGAGG ATGACGATGA ACAAGAAGAA AACTTAGAGG AACAGAACCC GGGGCTCTCT 
AAGAAGAAGG CGAGAGCTTC CCTGTCCGAC CTGTCAAGCC TTGAAGAGGT GGAAGGGATG 
AGCGTGCGCC AGCTGAAGGA AATCCTGGCT CGGAATTTTG TCAACTATTC TGGCTGCTGT 
GAAAAGTGGG AGCTGGTGGA GAAAGTCAAC CGGCTGTACA AGGAGAATGA GGAAAACCAA 
AAGTCATATG GTGAGCGGAT GCAACTTCAG GATGAGGAAG ACGACAGCCT GTGCCGAATC 
TGCATGGATG CGGTCATTGA CTGTGTCCTG CTGGAGTGTG GGCACATGGT CACCTGCACC 
AAGTGCGGCA AGCGCATGAG TGAGTGTCCC ATCTGCAGGC AGTATGTGGT TCGAGCTGTG 
CATGTTTTCA AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf34 ( XM_006249361.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249423.1 Rattus norvegicus ring finger protein 34 (Rnf34), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa35038
Clone ID Related Accession (Same CDS sequence)	XM_006249361.3 , XM_008769214.2
Accession Version	XM_006249361.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RNF34 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249361.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTT CATGCTGTGG ACTGCTGAAT GAAGTCATGG GAACTGGAGC TGTCAGAGGC 
CAGCAGGCAG GATTCCCAGG AAGCACTGGT CCATTCAGGT TTACACCGAG TTCTGACTTT 
CCTACCTACC CACCAGCAGC TACAGAAGGA CCCAATATAG TTTGCAAAGC CTGTGGACTT 
TCATTTTCAG TCTTTAGAAA GAAGCACGTC TGCTGTGACT GCAAGAAGGA CTTTTGCTCC 
CTTTGTTCGG TCTCACAAGA AAACCTCCGC AGATGCTCTA CATGTCACCT ACTCCAAGAG 
ACAGCCTTCC AGCGCCCTCA GTTGATGAGA CTAAAAGTGA AGGACCTGCG GCAGTATCTG 
CTCCTCAGAA ACGTACCGAC CGACACTTGT CGCGAGAAGG AGGACTTAGT GGATCTAGTG 
CTGTGCCATC GTGGACTGGG CTCGGGGGAT GGCCTGGACT CAAGGAGTCT CAGTTCCTCA 
AGATCCCAGA CTTCTAGTTT TTTTACACAA TCCTATTTTT CAAATTATAC ACCCCCCTCT 
GCTACTGTGT CCTCCTTCCA GGGAGAGCTG ATGGACAGAG AGGGCACCTT CAGGTCAGAA 
GTTCTCACAC AGGTACAAAG TGAATTAGCT TCAGCAAACA CGGATGATGA GGATGGGGAG 
GAAGATGACG ACGATGATGA CGATGACGAC GACGAGGATG ACGATGAACA AGAAGAAAAC 
TTAGAGGAAC AGAACCCGGG GCTCTCTAAG AAGAAGGCGA GAGCTTCCCT GTCCGACCTG 
TCAAGCCTTG AAGAGGTGGA AGGGATGAGC GTGCGCCAGC TGAAGGAAAT CCTGGCTCGG 
AATTTTGTCA ACTATTCTGG CTGCTGTGAA AAGTGGGAGC TGGTGGAGAA AGTCAACCGG 
CTGTACAAGG AGAATGAGGA AAACCAAAAG TCATATGGTG AGCGGATGCA ACTTCAGGAT 
GAGGAAGACG ACAGCCTGTG CCGAATCTGC ATGGATGCGG TCATTGACTG TGTCCTGCTG 
GAGTGTGGGC ACATGGTCAC CTGCACCAAG TGCGGCAAGC GCATGAGTGA GTGTCCCATC 
TGCAGGCAGT ATGTGGTTCG AGCTGTGCAT GTTTTCAAGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249423.1
CDS	324..1448
Translation	MWASCCGLLNEVMGTGAVRGQQAGFPGSTGPFRFTPSSDFPTYPPAATEGPNIVCKACGLSFSVFRKKHVCCDCKKDFCSLCSVSQENLRRCSTCHLLQETAFQRPQLMRLKVKDLRQYLLLRNVPTDTCREKEDLVDLVLCHRGLGSGDGLDSRSLSSSRSQTSSFFTQSYFSNYTPPSATVSSFQGELMDREGTFRSEVLTQVQSELASANTDDEDGEEDDDDDDDDDDEDDDEQEENLEEQNPGLSKKKARASLSDLSSLEEVEGMSVRQLKEILARNFVNYSGCCEKWELVEKVNRLYKENEENQKSYGERMQLQDEEDDSLCRICMDAVIDCVLLECGHMVTCTKCGKRMSECPICRQYVVRAVHVFKS

Target ORF information:

RefSeq Version	XM_006249361.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger protein 34 (Rnf34), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249361.3

ORF Insert Sequence:

ATGTGGGCTT CATGCTGTGG ACTGCTGAAT GAAGTCATGG GAACTGGAGC TGTCAGAGGC 
CAGCAGGCAG GATTCCCAGG AAGCACTGGT CCATTCAGGT TTACACCGAG TTCTGACTTT 
CCTACCTACC CACCAGCAGC TACAGAAGGA CCCAATATAG TTTGCAAAGC CTGTGGACTT 
TCATTTTCAG TCTTTAGAAA GAAGCACGTC TGCTGTGACT GCAAGAAGGA CTTTTGCTCC 
CTTTGTTCGG TCTCACAAGA AAACCTCCGC AGATGCTCTA CATGTCACCT ACTCCAAGAG 
ACAGCCTTCC AGCGCCCTCA GTTGATGAGA CTAAAAGTGA AGGACCTGCG GCAGTATCTG 
CTCCTCAGAA ACGTACCGAC CGACACTTGT CGCGAGAAGG AGGACTTAGT GGATCTAGTG 
CTGTGCCATC GTGGACTGGG CTCGGGGGAT GGCCTGGACT CAAGGAGTCT CAGTTCCTCA 
AGATCCCAGA CTTCTAGTTT TTTTACACAA TCCTATTTTT CAAATTATAC ACCCCCCTCT 
GCTACTGTGT CCTCCTTCCA GGGAGAGCTG ATGGACAGAG AGGGCACCTT CAGGTCAGAA 
GTTCTCACAC AGGTACAAAG TGAATTAGCT TCAGCAAACA CGGATGATGA GGATGGGGAG 
GAAGATGACG ACGATGATGA CGATGACGAC GACGAGGATG ACGATGAACA AGAAGAAAAC 
TTAGAGGAAC AGAACCCGGG GCTCTCTAAG AAGAAGGCGA GAGCTTCCCT GTCCGACCTG 
TCAAGCCTTG AAGAGGTGGA AGGGATGAGC GTGCGCCAGC TGAAGGAAAT CCTGGCTCGG 
AATTTTGTCA ACTATTCTGG CTGCTGTGAA AAGTGGGAGC TGGTGGAGAA AGTCAACCGG 
CTGTACAAGG AGAATGAGGA AAACCAAAAG TCATATGGTG AGCGGATGCA ACTTCAGGAT 
GAGGAAGACG ACAGCCTGTG CCGAATCTGC ATGGATGCGG TCATTGACTG TGTCCTGCTG 
GAGTGTGGGC ACATGGTCAC CTGCACCAAG TGCGGCAAGC GCATGAGTGA GTGTCCCATC 
TGCAGGCAGT ATGTGGTTCG AGCTGTGCAT GTTTTCAAGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf34 ( XM_008769214.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767436.1 Rattus norvegicus ring finger protein 34 (Rnf34), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa35038
Clone ID Related Accession (Same CDS sequence)	XM_006249361.3 , XM_008769214.2
Accession Version	XM_008769214.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RNF34 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769214.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTT CATGCTGTGG ACTGCTGAAT GAAGTCATGG GAACTGGAGC TGTCAGAGGC 
CAGCAGGCAG GATTCCCAGG AAGCACTGGT CCATTCAGGT TTACACCGAG TTCTGACTTT 
CCTACCTACC CACCAGCAGC TACAGAAGGA CCCAATATAG TTTGCAAAGC CTGTGGACTT 
TCATTTTCAG TCTTTAGAAA GAAGCACGTC TGCTGTGACT GCAAGAAGGA CTTTTGCTCC 
CTTTGTTCGG TCTCACAAGA AAACCTCCGC AGATGCTCTA CATGTCACCT ACTCCAAGAG 
ACAGCCTTCC AGCGCCCTCA GTTGATGAGA CTAAAAGTGA AGGACCTGCG GCAGTATCTG 
CTCCTCAGAA ACGTACCGAC CGACACTTGT CGCGAGAAGG AGGACTTAGT GGATCTAGTG 
CTGTGCCATC GTGGACTGGG CTCGGGGGAT GGCCTGGACT CAAGGAGTCT CAGTTCCTCA 
AGATCCCAGA CTTCTAGTTT TTTTACACAA TCCTATTTTT CAAATTATAC ACCCCCCTCT 
GCTACTGTGT CCTCCTTCCA GGGAGAGCTG ATGGACAGAG AGGGCACCTT CAGGTCAGAA 
GTTCTCACAC AGGTACAAAG TGAATTAGCT TCAGCAAACA CGGATGATGA GGATGGGGAG 
GAAGATGACG ACGATGATGA CGATGACGAC GACGAGGATG ACGATGAACA AGAAGAAAAC 
TTAGAGGAAC AGAACCCGGG GCTCTCTAAG AAGAAGGCGA GAGCTTCCCT GTCCGACCTG 
TCAAGCCTTG AAGAGGTGGA AGGGATGAGC GTGCGCCAGC TGAAGGAAAT CCTGGCTCGG 
AATTTTGTCA ACTATTCTGG CTGCTGTGAA AAGTGGGAGC TGGTGGAGAA AGTCAACCGG 
CTGTACAAGG AGAATGAGGA AAACCAAAAG TCATATGGTG AGCGGATGCA ACTTCAGGAT 
GAGGAAGACG ACAGCCTGTG CCGAATCTGC ATGGATGCGG TCATTGACTG TGTCCTGCTG 
GAGTGTGGGC ACATGGTCAC CTGCACCAAG TGCGGCAAGC GCATGAGTGA GTGTCCCATC 
TGCAGGCAGT ATGTGGTTCG AGCTGTGCAT GTTTTCAAGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767436.1
CDS	36..1160
Translation	MWASCCGLLNEVMGTGAVRGQQAGFPGSTGPFRFTPSSDFPTYPPAATEGPNIVCKACGLSFSVFRKKHVCCDCKKDFCSLCSVSQENLRRCSTCHLLQETAFQRPQLMRLKVKDLRQYLLLRNVPTDTCREKEDLVDLVLCHRGLGSGDGLDSRSLSSSRSQTSSFFTQSYFSNYTPPSATVSSFQGELMDREGTFRSEVLTQVQSELASANTDDEDGEEDDDDDDDDDDEDDDEQEENLEEQNPGLSKKKARASLSDLSSLEEVEGMSVRQLKEILARNFVNYSGCCEKWELVEKVNRLYKENEENQKSYGERMQLQDEEDDSLCRICMDAVIDCVLLECGHMVTCTKCGKRMSECPICRQYVVRAVHVFKS

Target ORF information:

RefSeq Version	XM_008769214.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger protein 34 (Rnf34), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769214.2

ORF Insert Sequence:

ATGTGGGCTT CATGCTGTGG ACTGCTGAAT GAAGTCATGG GAACTGGAGC TGTCAGAGGC 
CAGCAGGCAG GATTCCCAGG AAGCACTGGT CCATTCAGGT TTACACCGAG TTCTGACTTT 
CCTACCTACC CACCAGCAGC TACAGAAGGA CCCAATATAG TTTGCAAAGC CTGTGGACTT 
TCATTTTCAG TCTTTAGAAA GAAGCACGTC TGCTGTGACT GCAAGAAGGA CTTTTGCTCC 
CTTTGTTCGG TCTCACAAGA AAACCTCCGC AGATGCTCTA CATGTCACCT ACTCCAAGAG 
ACAGCCTTCC AGCGCCCTCA GTTGATGAGA CTAAAAGTGA AGGACCTGCG GCAGTATCTG 
CTCCTCAGAA ACGTACCGAC CGACACTTGT CGCGAGAAGG AGGACTTAGT GGATCTAGTG 
CTGTGCCATC GTGGACTGGG CTCGGGGGAT GGCCTGGACT CAAGGAGTCT CAGTTCCTCA 
AGATCCCAGA CTTCTAGTTT TTTTACACAA TCCTATTTTT CAAATTATAC ACCCCCCTCT 
GCTACTGTGT CCTCCTTCCA GGGAGAGCTG ATGGACAGAG AGGGCACCTT CAGGTCAGAA 
GTTCTCACAC AGGTACAAAG TGAATTAGCT TCAGCAAACA CGGATGATGA GGATGGGGAG 
GAAGATGACG ACGATGATGA CGATGACGAC GACGAGGATG ACGATGAACA AGAAGAAAAC 
TTAGAGGAAC AGAACCCGGG GCTCTCTAAG AAGAAGGCGA GAGCTTCCCT GTCCGACCTG 
TCAAGCCTTG AAGAGGTGGA AGGGATGAGC GTGCGCCAGC TGAAGGAAAT CCTGGCTCGG 
AATTTTGTCA ACTATTCTGG CTGCTGTGAA AAGTGGGAGC TGGTGGAGAA AGTCAACCGG 
CTGTACAAGG AGAATGAGGA AAACCAAAAG TCATATGGTG AGCGGATGCA ACTTCAGGAT 
GAGGAAGACG ACAGCCTGTG CCGAATCTGC ATGGATGCGG TCATTGACTG TGTCCTGCTG 
GAGTGTGGGC ACATGGTCAC CTGCACCAAG TGCGGCAAGC GCATGAGTGA GTGTCCCATC 
TGCAGGCAGT ATGTGGTTCG AGCTGTGCAT GTTTTCAAGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Altered Gene Expression of RNF34 and PACAP Possibly Involved in Mechanism of Exercise-Induced Analgesia for Neuropathic Pain in Rats.
International journal of molecular sciences18(9)(2017 Sep)
Yamaoka S,Oshima Y,Horiuchi H,Morino T,Hino M,Miura H,Ogata T

Ring finger protein 34 (RNF34) interacts with and promotes γ-aminobutyric acid type-A receptor degradation via ubiquitination of the γ2 subunit.
The Journal of biological chemistry289(42)29420-36(2014 Oct)
Jin H,Chiou TT,Serwanski DR,Miralles CP,Pinal N,De Blas AL

The E3 ligase RNF34 is a novel negative regulator of the NOD1 pathway.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology33(6)1954-62(2014)
Zhang R,Zhao J,Song Y,Wang X,Wang L,Xu J,Song C,Liu F

RNF34 is a cold-regulated E3 ubiquitin ligase for PGC-1α and modulates brown fat cell metabolism.
Molecular and cellular biology32(2)266-75(2012 Jan)
Wei P,Pan D,Mao C,Wang YX

CARPs enhance p53 turnover by degrading 14-3-3sigma and stabilizing MDM2.
Cell cycle (Georgetown, Tex.)7(5)670-82(2008 Mar)
Yang W,Dicker DT,Chen J,El-Deiry WS

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Rnf34 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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