Hspa5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hspa5
Entrez Gene ID	25617
Full Name	heat shock protein family A member 5
Synonyms	BIP,GRP 78,GRP78
General protein information	Preferred Names heat shock protein family A member 5 Names 78 kDa glucose-regulated protein GRP-78 heat shock 70 kDa protein 5 heat shock 70kD protein 5 heat shock 70kDa protein 5 (glucose-regulated protein) heat shock protein 5 immunoglobulin heavy chain-binding protein steroidogenesis-activator polypeptide
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p11
Summary	secreted protein of the endoplasmic reticulum; may be involved in the assembly of secreted and membrane-bound proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013083.2	NP_037215.1	endoplasmic reticulum chaperone BiP precursor

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	bip1	NP_593245.1
Caenorhabditis elegans (roundworm)	hsp-4	NP_495536.1
Arabidopsis thaliana (thale cress)	BIP1	NP_198206.1
Homo sapiens (human)	HSPA5	NP_005338.1
Rattus norvegicus (Norway rat)	Hspa5	NP_037215.1
Danio rerio (zebrafish)	hspa5	NP_998223.1
Neurospora crassa	NCU03982	XP_956567.1
Xenopus tropicalis (tropical clawed frog)	LOC100492570	XP_002941690.1
Macaca mulatta (Rhesus monkey)	HSPA5	XP_001099110.1
Canis lupus familiaris (dog)	HSPA5	XP_863385.2
Bos taurus (cattle)	HSPA5	NP_001068616.1
Eremothecium gossypii	AGOS_ACR038W	NP_983441.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004192	XP_313085.3
Kluyveromyces lactis	KLLA0D09559g	XP_453488.1
Caenorhabditis elegans (roundworm)	hsp-3	NP_509019.1
Saccharomyces cerevisiae (baker's yeast)	KAR2	NP_012500.3
Arabidopsis thaliana (thale cress)	BIP2	NP_851119.1
Magnaporthe oryzae (rice blast fungus)	MGG_02503	XP_003709308.1
Pan troglodytes (chimpanzee)	HSPA5	XP_520257.2
Mus musculus (house mouse)	Hspa5	NP_001156906.1
Gallus gallus (chicken)	HSPA5	NP_990822.1
Drosophila melanogaster (fruit fly)	Hsc70-3	NP_727564.1
Oryza sativa (rice)	Os02g0115900	NP_001045675.1

Gene Ontology
Bibliography

Related articles in PubMed

Myocardial Infarction-Related Transcripts (MIAT) Participate in Diabetic Optic Nerve Injury by Regulating Heart Shock Protein 5 (HSPA5) via Competitively Binding to MicroRNA-379.
Xu Y, Wang X, Zhang Y
Medical science monitor : international medical journal of experimental and clinical research252096-2103(2019 Mar)

miR-181b-5p suppresses starvation-induced cardiomyocyte autophagy by targeting Hspa5.
Chang L, Chai X, Chen P, Cao J, Xie H, Zhu J
International journal of molecular medicine43(1)143-154(2019 Jan)

Glucose-regulated protein 78 in lipid rafts elevates vascular smooth muscle cell proliferation of spontaneously hypertensive rats by controlling platelet-derived growth factor receptor signaling.
Kim DY, Hwang DI, Park SM, Jung SH, Kim B, Won KJ, Lee HM
Pflugers Archiv : European journal of physiology470(12)1831-1843(2018 12)

Chlorogenic acid against palmitic acid in endoplasmic reticulum stress-mediated apoptosis resulting in protective effect of primary rat hepatocytes.
Zhang Y, Miao L, Zhang H, Wu G, Zhang Z, Lv J
Lipids in health and disease17(1)270(2018 Nov)

Sevoflurane relieves hepatic ischemia-reperfusion injury by inhibiting the expression of Grp78.
Liu D, Jin X, Zhang C, Shang Y
Bioscience reports38(5)(2018 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hspa5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hspa5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12699	NM_013083.2 Latest version!	Rattus norvegicus heat shock protein family A (Hsp70) member 5 (Hspa5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hspa5 ( NM_013083.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037215.1 Rattus norvegicus heat shock protein family A (Hsp70) member 5 (Hspa5), mRNA.

Price & Availability↑

CloneID	ORa12699
Clone ID Related Accession (Same CDS sequence)	NM_013083.2
Accession Version	NM_013083.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Rattus norvegicus(Norway rat)
Product	endoplasmic reticulum chaperone BiP precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB578015.1, BC062017.1, M14050.1 and CB327067.1. On Mar 25, 2010 this sequence version replaced NM_013083.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062017.1, CF111168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTTCA CTGTGGTGGC GGCGGCGCTG CTGCTGCTGT GTGCGGTGCG GGCGGAGGAG 
GAGGACAAGA AGGAGGATGT AGGCACGGTG GTTGGCATCG ACTTGGGGAC CACCTATTCC 
TGCGTCGGTG TATTCAAGAA CGGCCGCGTG GAGATCATAG CCAACGATCA GGGCAACCGC 
ATCACACCGT CGTATGTGGC CTTCACTCCT GAAGGGGAGC GTCTGATTGG CGATGCGGCC 
AAGAACCAAC TCACGTCCAA CCCGGAGAAC ACCGTCTTCG ACGCCAAGCG CCTCATCGGA 
CGCACTTGGA ATGACCCTTC CGTGCAGCAG GACATCAAGT TCTTGCCATT CAAGGTGGTT 
GAAAAGAAAA CTAAACCATA TATTCAAGTT GATATCGGAG GTGGGCAAAC CAAGACATTT 
GCCCCAGAAG AAATTTCTGC TATGGTTCTC ACTAAAATGA AGGAAACTGC CGAGGCGTAT 
TTGGGAAAGA AGGTCACCCA TGCAGTTGTG ACTGTACCAG CTTACTTCAA TGATGCACAG 
CGGCAAGCAA CCAAGGATGC TGGCACTATT GCTGGACTGA ATGTCATGAG GATCATCAAT 
GAGCCAACAG CAGCTGCTAT TGCATATGGC CTGGATAAGA GAGAGGGAGA GAAGAACATC 
CTCGTGTTTG ACCTGGGTGG TGGAACCTTC GATGTGTCTC TTCTCACCAT TGACAATGGT 
GTCTTTGAAG TGGTGGCCAC TAATGGAGAC ACTCATCTGG GTGGGGAAGA CTTTGATCAG 
CGGGTCATGG AGCACTTCAT CAAGCTGTAC AAAAAGAAGA CTGGGAAAGA TGTCAGAAAG 
GACAACAGAG CTGTGCAGAA ACTCCGGCGT GAGGTAGAAA AGGCCAAGAG AGCCCTGTCT 
TCTCAGCATC AAGCGAGGAT TGAAATTGAG TCCTTCTTCG AAGGAGAAGA CTTCTCAGAG 
ACCCTTACTC GGGCCAAATT TGAAGAGCTG AACATGGACC TGTTCCGCTC TACCATGAAA 
CCTGTCCAGA AAGTGTTGGA AGACTCTGAT CTGAAGAAAT CTGATATTGA TGAGATTGTT 
CTGGTTGGCG GATCTACTCG AATTCCAAAG ATTCAGCAGT TGGTGAAAGA GTTCTTCAAT 
GGCAAGGAGC CGTCCCGTGG CATCAACCCA GATGAGGCTG TAGCATATGG TGCCGCTGTC 
CAGGCTGGTG TCCTCTCTGG TGATCAAGAT ACAGGTGACC TGGTTCTGCT TGATGTGTGT 
CCTCTTACAC TTGGTATTGA AACTGTGGGA GGTGTCATGA CAAAACTGAT TCCGAGGAAC 
ACTGTGGTGC CCACCAAGAA GTCTCAGATC TTCTCCACAG CTTCTGATAA TCAGCCCACC 
GTAACAATCA AGGTCTACGA AGGTGAACGA CCCCTGACAA AAGACAATCA CCTTCTGGGT 
ACATTTGATC TGACTGGAAT CCCTCCTGCT CCCCGTGGGG TACCCCAGAT TGAAGTCACC 
TTTGAGATAG ATGTGAATGG TATTCTTCGA GTGACAGCTG AAGACAAAGG GACAGGAAAC 
AAAAACAAAA TCACCATCAC CAATGACCAA AACCGCCTGA CCCCTGAAGA AATTGAAAGG 
ATGGTCAATG ACGCCGAGAA GTTTGCTGAG GAAGACAAAA AGCTCAAAGA GCGCATTGAC 
ACGAGGAATG AATTGGAAAG CTATGCTTAC TCTCTTAAGA ACCAGATCGG AGATAAAGAG 
AAGCTGGGAG GTAAGCTGTC TCCTGAAGAT AAAGAGACCA TGGAGAAAGC TGTAGAGGAA 
AAGATCGAAT GGCTGGAAAG CCACCAGGAT GCAGACATTG AAGACTTTAA AGCTAAAAAG 
AAGGAACTAG AAGAAATTGT TCAGCCAATT ATCAGCAAAC TCTATGGAAG TGGAGGCCCT 
CCCCCAACTG GTGAAGAGGA TACATCAGAA AAAGATGAGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037215.1
CDS	182..2146
Translation	MKFTVVAAALLLLCAVRAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSPEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSGGPPPTGEEDTSEKDEL

Target ORF information:

RefSeq Version	NM_013083.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus heat shock protein family A (Hsp70) member 5 (Hspa5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013083.2

ORF Insert Sequence:

ATGAAGTTCA CTGTGGTGGC GGCGGCGCTG CTGCTGCTGT GTGCGGTGCG GGCGGAGGAG 
GAGGACAAGA AGGAGGATGT AGGCACGGTG GTTGGCATCG ACTTGGGGAC CACCTATTCC 
TGCGTCGGTG TATTCAAGAA CGGCCGCGTG GAGATCATAG CCAACGATCA GGGCAACCGC 
ATCACACCGT CGTATGTGGC CTTCACTCCT GAAGGGGAGC GTCTGATTGG CGATGCGGCC 
AAGAACCAAC TCACGTCCAA CCCGGAGAAC ACCGTCTTCG ACGCCAAGCG CCTCATCGGA 
CGCACTTGGA ATGACCCTTC CGTGCAGCAG GACATCAAGT TCTTGCCATT CAAGGTGGTT 
GAAAAGAAAA CTAAACCATA TATTCAAGTT GATATCGGAG GTGGGCAAAC CAAGACATTT 
GCCCCAGAAG AAATTTCTGC TATGGTTCTC ACTAAAATGA AGGAAACTGC CGAGGCGTAT 
TTGGGAAAGA AGGTCACCCA TGCAGTTGTG ACTGTACCAG CTTACTTCAA TGATGCACAG 
CGGCAAGCAA CCAAGGATGC TGGCACTATT GCTGGACTGA ATGTCATGAG GATCATCAAT 
GAGCCAACAG CAGCTGCTAT TGCATATGGC CTGGATAAGA GAGAGGGAGA GAAGAACATC 
CTCGTGTTTG ACCTGGGTGG TGGAACCTTC GATGTGTCTC TTCTCACCAT TGACAATGGT 
GTCTTTGAAG TGGTGGCCAC TAATGGAGAC ACTCATCTGG GTGGGGAAGA CTTTGATCAG 
CGGGTCATGG AGCACTTCAT CAAGCTGTAC AAAAAGAAGA CTGGGAAAGA TGTCAGAAAG 
GACAACAGAG CTGTGCAGAA ACTCCGGCGT GAGGTAGAAA AGGCCAAGAG AGCCCTGTCT 
TCTCAGCATC AAGCGAGGAT TGAAATTGAG TCCTTCTTCG AAGGAGAAGA CTTCTCAGAG 
ACCCTTACTC GGGCCAAATT TGAAGAGCTG AACATGGACC TGTTCCGCTC TACCATGAAA 
CCTGTCCAGA AAGTGTTGGA AGACTCTGAT CTGAAGAAAT CTGATATTGA TGAGATTGTT 
CTGGTTGGCG GATCTACTCG AATTCCAAAG ATTCAGCAGT TGGTGAAAGA GTTCTTCAAT 
GGCAAGGAGC CGTCCCGTGG CATCAACCCA GATGAGGCTG TAGCATATGG TGCCGCTGTC 
CAGGCTGGTG TCCTCTCTGG TGATCAAGAT ACAGGTGACC TGGTTCTGCT TGATGTGTGT 
CCTCTTACAC TTGGTATTGA AACTGTGGGA GGTGTCATGA CAAAACTGAT TCCGAGGAAC 
ACTGTGGTGC CCACCAAGAA GTCTCAGATC TTCTCCACAG CTTCTGATAA TCAGCCCACC 
GTAACAATCA AGGTCTACGA AGGTGAACGA CCCCTGACAA AAGACAATCA CCTTCTGGGT 
ACATTTGATC TGACTGGAAT CCCTCCTGCT CCCCGTGGGG TACCCCAGAT TGAAGTCACC 
TTTGAGATAG ATGTGAATGG TATTCTTCGA GTGACAGCTG AAGACAAAGG GACAGGAAAC 
AAAAACAAAA TCACCATCAC CAATGACCAA AACCGCCTGA CCCCTGAAGA AATTGAAAGG 
ATGGTCAATG ACGCCGAGAA GTTTGCTGAG GAAGACAAAA AGCTCAAAGA GCGCATTGAC 
ACGAGGAATG AATTGGAAAG CTATGCTTAC TCTCTTAAGA ACCAGATCGG AGATAAAGAG 
AAGCTGGGAG GTAAGCTGTC TCCTGAAGAT AAAGAGACCA TGGAGAAAGC TGTAGAGGAA 
AAGATCGAAT GGCTGGAAAG CCACCAGGAT GCAGACATTG AAGACTTTAA AGCTAAAAAG 
AAGGAACTAG AAGAAATTGT TCAGCCAATT ATCAGCAAAC TCTATGGAAG TGGAGGCCCT 
CCCCCAACTG GTGAAGAGGA TACATCAGAA AAAGATGAGT TGTAG