Psap cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Psap
Entrez Gene ID	25524
Full Name	prosaposin
Synonyms	SGP-1,SGP1A
General protein information	Preferred Names prosaposin Names prosaposin prosaposin (sulfated glycoprotein, sphingolipid hydrolase activator) sulfated glycoprotein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q11
Summary	may play a role in glycolipid transport during spermatogenesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001190238.1	NP_001177167.1	prosaposin isoform D preproprotein
NM_001190236.1	NP_001177165.1	prosaposin isoform B preproprotein
NM_001190237.1	NP_001177166.1	prosaposin isoform C preproprotein
XM_006256403.1	XP_006256465.1	prosaposin isoform X1
NM_013013.2	NP_037145.2	prosaposin isoform A preproprotein

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	PSAP	XP_001107102.1
Mus musculus (house mouse)	Psap	NP_001139593.1
Gallus gallus (chicken)	PSAP	NP_990142.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001082	XP_003437209.1
Drosophila melanogaster (fruit fly)	Sap-r	NP_524597.1
Xenopus tropicalis (tropical clawed frog)	psap	NP_001017285.1
Canis lupus familiaris (dog)	PSAP	XP_005619049.1
Bos taurus (cattle)	PSAP	NP_776586.1
Rattus norvegicus (Norway rat)	Psap	NP_001177166.1
Danio rerio (zebrafish)	psap	NP_571958.1
Homo sapiens (human)	PSAP	NP_001035931.1
Pan troglodytes (chimpanzee)	PSAP	XP_507840.2

Gene Ontology
Bibliography

Related articles in PubMed

Prosaposin and its receptors are differentially expressed in the salivary glands of male and female rats.
Islam F, Khan MSI, Nabeka H, Saito S, Li X, Shimokawa T, Yamamiya K, Kobayashi N, Matsuda S
Cell and tissue research373(2)439-457(2018 08)

Lysosomal processing of progranulin.
Zhou X, Paushter DH, Feng T, Sun L, Reinheckel T, Hu F
Molecular neurodegeneration12(1)62(2017 08)

Impaired prosaposin lysosomal trafficking in frontotemporal lobar degeneration due to progranulin mutations.
Zhou X, Sun L, Bracko O, Choi JW, Jia Y, Nana AL, Brady OA, Hernandez JCC, Nishimura N, Seeley WW, Hu F
Nature communications815277(2017 05)

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Psap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Psap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01646	NM_013013.2 Latest version!	Rattus norvegicus prosaposin (Psap), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa06763	NM_001190236.1 Latest version!	Rattus norvegicus prosaposin (Psap), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa06764	NM_001190237.1 Latest version!	Rattus norvegicus prosaposin (Psap), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa06766	NM_001190238.1 Latest version!	Rattus norvegicus prosaposin (Psap), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa39432	XM_006256403.1 Latest version!	Rattus norvegicus prosaposin (Psap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Psap ( NM_013013.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037145.2 Rattus norvegicus prosaposin (Psap), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa01646
Clone ID Related Accession (Same CDS sequence)	NM_013013.2
Accession Version	NM_013013.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	prosaposin isoform A preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK365248.1, M19936.1, DY313840.1, BC061759.1 and CO558861.1. On Jun 13, 2010 this sequence version replaced NM_013013.1. Transcript Variant: This variant (1) lacks an exon in the coding region, compared to variant 2, resulting in a shorter protein (isoform A), compared to isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ217536.1, M19936.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATGCTC TCGCTCTCCT CGCCAGCCTT CTGGTCACCG CTCTGACCAG CCCTGTCCAG 
GACCCGAAGA TCTGCTCTGG GGGCTCAGCA GTAGTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTAGGG CTGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCAAAATCC 
CTTCCTTGTG ACATATGCAA AACGGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACTGAGG AGGAGATCCT CCATTACCTG GAGAAGACCT GTGCGTGGAT TCATGACTCC 
AGCCTGTCAG CCTCTTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGGG AGATGAGCAA CCCCGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AGACAGCTGG AGTCCAACAA GATCCCGGAG 
GTGGACCTGG CCCGCGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCGGC CTCGCAGCCA GCCGCAGCCC AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGTTGG TGACTGACAT CCAGACTGCT GTGAGGACCA ACTCCAGCTT TGTCCAGGGC 
TTGGTGGACC ACGTCAAGGA GGATTGTGAC CGCCTCGGGC CAGGCGTGTC TGACATATGC 
AAGAACTATG TTGACCAGTA TTCTGAGGTC GCCGTCCAGA TGATGATGCA CATGCAACCC 
AAGGAAATCT GTGTGATGGT TGGCTTCTGT GATGAGGTCA AGAGAGTGCC AATGAGGACT 
CTGGTCCCCG CCACTGAGGC CATCAAGAAT ATCCTCCCTG CCCTGGAGCT GACGGACCCC 
TATGAGCAGG ATGTGATCCA GGCCCAAAAT GTGATTTTCT GCCAAGTTTG TCAGCTTGTG 
ATGCGCAAGT TGTCTGAACT GATTATCAAC AATGCCACTG AGGAACTTCT AATTAAAGGT 
TTGAGTAAAG CCTGCTCCCT GCTCCCTGCT CCTGCTTCAA CCAAGTGCCA GGAAGTGCTG 
GTAACATTTG GCCCCTCTCT GTTGGACGTC CTCATGCATG AGGTGAACCC GAACTTTCTG 
TGCGGTGTGA TCAGCCTCTG TTCTGCCAAC CCGAATTTGG TGGGGACCCT TGAACAACCT 
GCAGCAGCCA TTGTATCTGC ACTGCCCAAA GAGCCTGCAC CGCCAAAACA GCCTGAAGAG 
CCCAAGCAGT CTGCATTGCG CGCCCATGTG CCTCCTCAGA AGAACGGGGG GTTCTGTGAG 
GTGTGCAAGA AGCTGGTCAT CTATTTGGAA CATAACCTGG AGAAAAACAG CACCAAGGAG 
GAGATCCTGG CTGCACTTGA GAAGGGCTGC AGCTTCCTGC CAGACCCTTA CCAGAAGCAG 
TGTGATGAAT TTGTGGCTGA GTATGAGCCC TTACTGCTGG AAATCCTTGT GGAGGTGATG 
GATCCTTCCT TTGTGTGCTC GAAAATTGGA GTCTGCCCTT CTGCCTATAA GCTGCTGCTG 
GGAACCGAGA AGTGTGTCTG GGGCCCAGGC TACTGGTGTC AGAACATGGA GACTGCTGCC 
CGATGCAATG CTGTCGATCA TTGCAAACGC CATGTGTGGA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037145.2
CDS	78..1742
Translation	MYALALLASLLVTALTSPVQDPKICSGGSAVVCRDVKTAVDCRAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATEEEILHYLEKTCAWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQRQLESNKIPEVDLARVVAPFMSNIPLLLYPQDRPRSQPQPKANEDVCQDCMKLVTDIQTAVRTNSSFVQGLVDHVKEDCDRLGPGVSDICKNYVDQYSEVAVQMMMHMQPKEICVMVGFCDEVKRVPMRTLVPATEAIKNILPALELTDPYEQDVIQAQNVIFCQVCQLVMRKLSELIINNATEELLIKGLSKACSLLPAPASTKCQEVLVTFGPSLLDVLMHEVNPNFLCGVISLCSANPNLVGTLEQPAAAIVSALPKEPAPPKQPEEPKQSALRAHVPPQKNGGFCEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVEVMDPSFVCSKIGVCPSAYKLLLGTEKCVWGPGYWCQNMETAARCNAVDHCKRHVWN

Target ORF information:

RefSeq Version	NM_013013.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prosaposin (Psap), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013013.2

ORF Insert Sequence:

ATGTATGCTC TCGCTCTCCT CGCCAGCCTT CTGGTCACCG CTCTGACCAG CCCTGTCCAG 
GACCCGAAGA TCTGCTCTGG GGGCTCAGCA GTAGTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTAGGG CTGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCAAAATCC 
CTTCCTTGTG ACATATGCAA AACGGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACTGAGG AGGAGATCCT CCATTACCTG GAGAAGACCT GTGCGTGGAT TCATGACTCC 
AGCCTGTCAG CCTCTTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGGG AGATGAGCAA CCCCGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AGACAGCTGG AGTCCAACAA GATCCCGGAG 
GTGGACCTGG CCCGCGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCGGC CTCGCAGCCA GCCGCAGCCC AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGTTGG TGACTGACAT CCAGACTGCT GTGAGGACCA ACTCCAGCTT TGTCCAGGGC 
TTGGTGGACC ACGTCAAGGA GGATTGTGAC CGCCTCGGGC CAGGCGTGTC TGACATATGC 
AAGAACTATG TTGACCAGTA TTCTGAGGTC GCCGTCCAGA TGATGATGCA CATGCAACCC 
AAGGAAATCT GTGTGATGGT TGGCTTCTGT GATGAGGTCA AGAGAGTGCC AATGAGGACT 
CTGGTCCCCG CCACTGAGGC CATCAAGAAT ATCCTCCCTG CCCTGGAGCT GACGGACCCC 
TATGAGCAGG ATGTGATCCA GGCCCAAAAT GTGATTTTCT GCCAAGTTTG TCAGCTTGTG 
ATGCGCAAGT TGTCTGAACT GATTATCAAC AATGCCACTG AGGAACTTCT AATTAAAGGT 
TTGAGTAAAG CCTGCTCCCT GCTCCCTGCT CCTGCTTCAA CCAAGTGCCA GGAAGTGCTG 
GTAACATTTG GCCCCTCTCT GTTGGACGTC CTCATGCATG AGGTGAACCC GAACTTTCTG 
TGCGGTGTGA TCAGCCTCTG TTCTGCCAAC CCGAATTTGG TGGGGACCCT TGAACAACCT 
GCAGCAGCCA TTGTATCTGC ACTGCCCAAA GAGCCTGCAC CGCCAAAACA GCCTGAAGAG 
CCCAAGCAGT CTGCATTGCG CGCCCATGTG CCTCCTCAGA AGAACGGGGG GTTCTGTGAG 
GTGTGCAAGA AGCTGGTCAT CTATTTGGAA CATAACCTGG AGAAAAACAG CACCAAGGAG 
GAGATCCTGG CTGCACTTGA GAAGGGCTGC AGCTTCCTGC CAGACCCTTA CCAGAAGCAG 
TGTGATGAAT TTGTGGCTGA GTATGAGCCC TTACTGCTGG AAATCCTTGT GGAGGTGATG 
GATCCTTCCT TTGTGTGCTC GAAAATTGGA GTCTGCCCTT CTGCCTATAA GCTGCTGCTG 
GGAACCGAGA AGTGTGTCTG GGGCCCAGGC TACTGGTGTC AGAACATGGA GACTGCTGCC 
CGATGCAATG CTGTCGATCA TTGCAAACGC CATGTGTGGA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psap ( NM_001190236.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001177165.1 Rattus norvegicus prosaposin (Psap), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa06763
Clone ID Related Accession (Same CDS sequence)	NM_001190236.1
Accession Version	NM_001190236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	prosaposin isoform B preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK365248.1, CO403947.1, M19936.1, DY313840.1, BC061759.1 and CO558861.1. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (B). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATGCTC TCGCTCTCCT CGCCAGCCTT CTGGTCACCG CTCTGACCAG CCCTGTCCAG 
GACCCGAAGA TCTGCTCTGG GGGCTCAGCA GTAGTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTAGGG CTGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCAAAATCC 
CTTCCTTGTG ACATATGCAA AACGGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACTGAGG AGGAGATCCT CCATTACCTG GAGAAGACCT GTGCGTGGAT TCATGACTCC 
AGCCTGTCAG CCTCTTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGGG AGATGAGCAA CCCCGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AGACAGCTGG AGTCCAACAA GATCCCGGAG 
GTGGACCTGG CCCGCGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCGGC CTCGCAGCCA GCCGCAGCCC AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGTTGG TGACTGACAT CCAGACTGCT GTGAGGACCA ACTCCAGCTT TGTCCAGGGC 
TTGGTGGACC ACGTCAAGGA GGATTGTGAC CGCCTCGGGC CAGGCGTGTC TGACATATGC 
AAGAACTATG TTGACCAGTA TTCTGAGGTC GCCGTCCAGA TGATGATGCA CATGCAGGAT 
CAGCAACCCA AGGAAATCTG TGTGATGGTT GGCTTCTGTG ATGAGGTCAA GAGAGTGCCA 
ATGAGGACTC TGGTCCCCGC CACTGAGGCC ATCAAGAATA TCCTCCCTGC CCTGGAGCTG 
ACGGACCCCT ATGAGCAGGA TGTGATCCAG GCCCAAAATG TGATTTTCTG CCAAGTTTGT 
CAGCTTGTGA TGCGCAAGTT GTCTGAACTG ATTATCAACA ATGCCACTGA GGAACTTCTA 
ATTAAAGGTT TGAGTAAAGC CTGCTCCCTG CTCCCTGCTC CTGCTTCAAC CAAGTGCCAG 
GAAGTGCTGG TAACATTTGG CCCCTCTCTG TTGGACGTCC TCATGCATGA GGTGAACCCG 
AACTTTCTGT GCGGTGTGAT CAGCCTCTGT TCTGCCAACC CGAATTTGGT GGGGACCCTT 
GAACAACCTG CAGCAGCCAT TGTATCTGCA CTGCCCAAAG AGCCTGCACC GCCAAAACAG 
CCTGAAGAGC CCAAGCAGTC TGCATTGCGC GCCCATGTGC CTCCTCAGAA GAACGGGGGG 
TTCTGTGAGG TGTGCAAGAA GCTGGTCATC TATTTGGAAC ATAACCTGGA GAAAAACAGC 
ACCAAGGAGG AGATCCTGGC TGCACTTGAG AAGGGCTGCA GCTTCCTGCC AGACCCTTAC 
CAGAAGCAGT GTGATGAATT TGTGGCTGAG TATGAGCCCT TACTGCTGGA AATCCTTGTG 
GAGGTGATGG ATCCTTCCTT TGTGTGCTCG AAAATTGGAG TCTGCCCTTC TGCCTATAAG 
CTGCTGCTGG GAACCGAGAA GTGTGTCTGG GGCCCAGGCT ACTGGTGTCA GAACATGGAG 
ACTGCTGCCC GATGCAATGC TGTCGATCAT TGCAAACGCC ATGTGTGGAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177165.1
CDS	78..1751
Translation	MYALALLASLLVTALTSPVQDPKICSGGSAVVCRDVKTAVDCRAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATEEEILHYLEKTCAWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQRQLESNKIPEVDLARVVAPFMSNIPLLLYPQDRPRSQPQPKANEDVCQDCMKLVTDIQTAVRTNSSFVQGLVDHVKEDCDRLGPGVSDICKNYVDQYSEVAVQMMMHMQDQQPKEICVMVGFCDEVKRVPMRTLVPATEAIKNILPALELTDPYEQDVIQAQNVIFCQVCQLVMRKLSELIINNATEELLIKGLSKACSLLPAPASTKCQEVLVTFGPSLLDVLMHEVNPNFLCGVISLCSANPNLVGTLEQPAAAIVSALPKEPAPPKQPEEPKQSALRAHVPPQKNGGFCEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVEVMDPSFVCSKIGVCPSAYKLLLGTEKCVWGPGYWCQNMETAARCNAVDHCKRHVWN

Target ORF information:

RefSeq Version	NM_001190236.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prosaposin (Psap), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190236.1

ORF Insert Sequence:

ATGTATGCTC TCGCTCTCCT CGCCAGCCTT CTGGTCACCG CTCTGACCAG CCCTGTCCAG 
GACCCGAAGA TCTGCTCTGG GGGCTCAGCA GTAGTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTAGGG CTGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCAAAATCC 
CTTCCTTGTG ACATATGCAA AACGGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACTGAGG AGGAGATCCT CCATTACCTG GAGAAGACCT GTGCGTGGAT TCATGACTCC 
AGCCTGTCAG CCTCTTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGGG AGATGAGCAA CCCCGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AGACAGCTGG AGTCCAACAA GATCCCGGAG 
GTGGACCTGG CCCGCGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCGGC CTCGCAGCCA GCCGCAGCCC AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGTTGG TGACTGACAT CCAGACTGCT GTGAGGACCA ACTCCAGCTT TGTCCAGGGC 
TTGGTGGACC ACGTCAAGGA GGATTGTGAC CGCCTCGGGC CAGGCGTGTC TGACATATGC 
AAGAACTATG TTGACCAGTA TTCTGAGGTC GCCGTCCAGA TGATGATGCA CATGCAGGAT 
CAGCAACCCA AGGAAATCTG TGTGATGGTT GGCTTCTGTG ATGAGGTCAA GAGAGTGCCA 
ATGAGGACTC TGGTCCCCGC CACTGAGGCC ATCAAGAATA TCCTCCCTGC CCTGGAGCTG 
ACGGACCCCT ATGAGCAGGA TGTGATCCAG GCCCAAAATG TGATTTTCTG CCAAGTTTGT 
CAGCTTGTGA TGCGCAAGTT GTCTGAACTG ATTATCAACA ATGCCACTGA GGAACTTCTA 
ATTAAAGGTT TGAGTAAAGC CTGCTCCCTG CTCCCTGCTC CTGCTTCAAC CAAGTGCCAG 
GAAGTGCTGG TAACATTTGG CCCCTCTCTG TTGGACGTCC TCATGCATGA GGTGAACCCG 
AACTTTCTGT GCGGTGTGAT CAGCCTCTGT TCTGCCAACC CGAATTTGGT GGGGACCCTT 
GAACAACCTG CAGCAGCCAT TGTATCTGCA CTGCCCAAAG AGCCTGCACC GCCAAAACAG 
CCTGAAGAGC CCAAGCAGTC TGCATTGCGC GCCCATGTGC CTCCTCAGAA GAACGGGGGG 
TTCTGTGAGG TGTGCAAGAA GCTGGTCATC TATTTGGAAC ATAACCTGGA GAAAAACAGC 
ACCAAGGAGG AGATCCTGGC TGCACTTGAG AAGGGCTGCA GCTTCCTGCC AGACCCTTAC 
CAGAAGCAGT GTGATGAATT TGTGGCTGAG TATGAGCCCT TACTGCTGGA AATCCTTGTG 
GAGGTGATGG ATCCTTCCTT TGTGTGCTCG AAAATTGGAG TCTGCCCTTC TGCCTATAAG 
CTGCTGCTGG GAACCGAGAA GTGTGTCTGG GGCCCAGGCT ACTGGTGTCA GAACATGGAG 
ACTGCTGCCC GATGCAATGC TGTCGATCAT TGCAAACGCC ATGTGTGGAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psap ( NM_001190237.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001177166.1 Rattus norvegicus prosaposin (Psap), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa06764
Clone ID Related Accession (Same CDS sequence)	NM_001190237.1
Accession Version	NM_001190237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	prosaposin isoform C preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK365248.1, BC061759.1, CB717025.1, DY313840.1 and CO558861.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region, compared to variant 2, resulting in a shorter protein (isoform C), compared to isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATGCTC TCGCTCTCCT CGCCAGCCTT CTGGTCACCG CTCTGACCAG CCCTGTCCAG 
GACCCGAAGA TCTGCTCTGG GGGCTCAGCA GTAGTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTAGGG CTGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCAAAATCC 
CTTCCTTGTG ACATATGCAA AACGGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACTGAGG AGGAGATCCT CCATTACCTG GAGAAGACCT GTGCGTGGAT TCATGACTCC 
AGCCTGTCAG CCTCTTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGGG AGATGAGCAA CCCCGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AGACAGCTGG AGTCCAACAA GATCCCGGAG 
GTGGACCTGG CCCGCGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCGGC CTCGCAGCCA GCCGCAGCCC AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGTTGG TGACTGACAT CCAGACTGCT GTGAGGACCA ACTCCAGCTT TGTCCAGGGC 
TTGGTGGACC ACGTCAAGGA GGATTGTGAC CGCCTCGGGC CAGGCGTGTC TGACATATGC 
AAGAACTATG TTGACCAGTA TTCTGAGGTC GCCGTCCAGA TGATGATGCA CATGCAGGAT 
CAGCAACCCA AGGAAATCTG TGTGATGGTT GGCTTCTGTG ATGAGGTCAA GAGAGTGCCA 
ATGAGGACTC TGGTCCCCGC CACTGAGGCC ATCAAGAATA TCCTCCCTGC CCTGGAGCTG 
ACGGACCCCT ATGAGGATGT GATCCAGGCC CAAAATGTGA TTTTCTGCCA AGTTTGTCAG 
CTTGTGATGC GCAAGTTGTC TGAACTGATT ATCAACAATG CCACTGAGGA ACTTCTAATT 
AAAGGTTTGA GTAAAGCCTG CTCCCTGCTC CCTGCTCCTG CTTCAACCAA GTGCCAGGAA 
GTGCTGGTAA CATTTGGCCC CTCTCTGTTG GACGTCCTCA TGCATGAGGT GAACCCGAAC 
TTTCTGTGCG GTGTGATCAG CCTCTGTTCT GCCAACCCGA ATTTGGTGGG GACCCTTGAA 
CAACCTGCAG CAGCCATTGT ATCTGCACTG CCCAAAGAGC CTGCACCGCC AAAACAGCCT 
GAAGAGCCCA AGCAGTCTGC ATTGCGCGCC CATGTGCCTC CTCAGAAGAA CGGGGGGTTC 
TGTGAGGTGT GCAAGAAGCT GGTCATCTAT TTGGAACATA ACCTGGAGAA AAACAGCACC 
AAGGAGGAGA TCCTGGCTGC ACTTGAGAAG GGCTGCAGCT TCCTGCCAGA CCCTTACCAG 
AAGCAGTGTG ATGAATTTGT GGCTGAGTAT GAGCCCTTAC TGCTGGAAAT CCTTGTGGAG 
GTGATGGATC CTTCCTTTGT GTGCTCGAAA ATTGGAGTCT GCCCTTCTGC CTATAAGCTG 
CTGCTGGGAA CCGAGAAGTG TGTCTGGGGC CCAGGCTACT GGTGTCAGAA CATGGAGACT 
GCTGCCCGAT GCAATGCTGT CGATCATTGC AAACGCCATG TGTGGAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177166.1
CDS	78..1748
Translation	MYALALLASLLVTALTSPVQDPKICSGGSAVVCRDVKTAVDCRAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATEEEILHYLEKTCAWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQRQLESNKIPEVDLARVVAPFMSNIPLLLYPQDRPRSQPQPKANEDVCQDCMKLVTDIQTAVRTNSSFVQGLVDHVKEDCDRLGPGVSDICKNYVDQYSEVAVQMMMHMQDQQPKEICVMVGFCDEVKRVPMRTLVPATEAIKNILPALELTDPYEDVIQAQNVIFCQVCQLVMRKLSELIINNATEELLIKGLSKACSLLPAPASTKCQEVLVTFGPSLLDVLMHEVNPNFLCGVISLCSANPNLVGTLEQPAAAIVSALPKEPAPPKQPEEPKQSALRAHVPPQKNGGFCEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVEVMDPSFVCSKIGVCPSAYKLLLGTEKCVWGPGYWCQNMETAARCNAVDHCKRHVWN

Target ORF information:

RefSeq Version	NM_001190237.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prosaposin (Psap), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190237.1

ORF Insert Sequence:

ATGTATGCTC TCGCTCTCCT CGCCAGCCTT CTGGTCACCG CTCTGACCAG CCCTGTCCAG 
GACCCGAAGA TCTGCTCTGG GGGCTCAGCA GTAGTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTAGGG CTGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCAAAATCC 
CTTCCTTGTG ACATATGCAA AACGGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACTGAGG AGGAGATCCT CCATTACCTG GAGAAGACCT GTGCGTGGAT TCATGACTCC 
AGCCTGTCAG CCTCTTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGGG AGATGAGCAA CCCCGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AGACAGCTGG AGTCCAACAA GATCCCGGAG 
GTGGACCTGG CCCGCGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCGGC CTCGCAGCCA GCCGCAGCCC AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGTTGG TGACTGACAT CCAGACTGCT GTGAGGACCA ACTCCAGCTT TGTCCAGGGC 
TTGGTGGACC ACGTCAAGGA GGATTGTGAC CGCCTCGGGC CAGGCGTGTC TGACATATGC 
AAGAACTATG TTGACCAGTA TTCTGAGGTC GCCGTCCAGA TGATGATGCA CATGCAGGAT 
CAGCAACCCA AGGAAATCTG TGTGATGGTT GGCTTCTGTG ATGAGGTCAA GAGAGTGCCA 
ATGAGGACTC TGGTCCCCGC CACTGAGGCC ATCAAGAATA TCCTCCCTGC CCTGGAGCTG 
ACGGACCCCT ATGAGGATGT GATCCAGGCC CAAAATGTGA TTTTCTGCCA AGTTTGTCAG 
CTTGTGATGC GCAAGTTGTC TGAACTGATT ATCAACAATG CCACTGAGGA ACTTCTAATT 
AAAGGTTTGA GTAAAGCCTG CTCCCTGCTC CCTGCTCCTG CTTCAACCAA GTGCCAGGAA 
GTGCTGGTAA CATTTGGCCC CTCTCTGTTG GACGTCCTCA TGCATGAGGT GAACCCGAAC 
TTTCTGTGCG GTGTGATCAG CCTCTGTTCT GCCAACCCGA ATTTGGTGGG GACCCTTGAA 
CAACCTGCAG CAGCCATTGT ATCTGCACTG CCCAAAGAGC CTGCACCGCC AAAACAGCCT 
GAAGAGCCCA AGCAGTCTGC ATTGCGCGCC CATGTGCCTC CTCAGAAGAA CGGGGGGTTC 
TGTGAGGTGT GCAAGAAGCT GGTCATCTAT TTGGAACATA ACCTGGAGAA AAACAGCACC 
AAGGAGGAGA TCCTGGCTGC ACTTGAGAAG GGCTGCAGCT TCCTGCCAGA CCCTTACCAG 
AAGCAGTGTG ATGAATTTGT GGCTGAGTAT GAGCCCTTAC TGCTGGAAAT CCTTGTGGAG 
GTGATGGATC CTTCCTTTGT GTGCTCGAAA ATTGGAGTCT GCCCTTCTGC CTATAAGCTG 
CTGCTGGGAA CCGAGAAGTG TGTCTGGGGC CCAGGCTACT GGTGTCAGAA CATGGAGACT 
GCTGCCCGAT GCAATGCTGT CGATCATTGC AAACGCCATG TGTGGAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psap ( NM_001190238.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001177167.1 Rattus norvegicus prosaposin (Psap), transcript variant 4, mRNA.

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CloneID	ORa06766
Clone ID Related Accession (Same CDS sequence)	NM_001190238.1
Accession Version	NM_001190238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	prosaposin isoform D preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK365248.1, BC061759.1, DY313840.1 and CO558861.1. Transcript Variant: This variant (4) lacks an exon in the coding region and uses an alternate in-frame splice site, compared to variant 2, resulting in a shorter protein (isoform D), compared to isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061759.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATGCTC TCGCTCTCCT CGCCAGCCTT CTGGTCACCG CTCTGACCAG CCCTGTCCAG 
GACCCGAAGA TCTGCTCTGG GGGCTCAGCA GTAGTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTAGGG CTGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCAAAATCC 
CTTCCTTGTG ACATATGCAA AACGGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACTGAGG AGGAGATCCT CCATTACCTG GAGAAGACCT GTGCGTGGAT TCATGACTCC 
AGCCTGTCAG CCTCTTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGGG AGATGAGCAA CCCCGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AGACAGCTGG AGTCCAACAA GATCCCGGAG 
GTGGACCTGG CCCGCGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCGGC CTCGCAGCCA GCCGCAGCCC AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGTTGG TGACTGACAT CCAGACTGCT GTGAGGACCA ACTCCAGCTT TGTCCAGGGC 
TTGGTGGACC ACGTCAAGGA GGATTGTGAC CGCCTCGGGC CAGGCGTGTC TGACATATGC 
AAGAACTATG TTGACCAGTA TTCTGAGGTC GCCGTCCAGA TGATGATGCA CATGCAACCC 
AAGGAAATCT GTGTGATGGT TGGCTTCTGT GATGAGGTCA AGAGAGTGCC AATGAGGACT 
CTGGTCCCCG CCACTGAGGC CATCAAGAAT ATCCTCCCTG CCCTGGAGCT GACGGACCCC 
TATGAGGATG TGATCCAGGC CCAAAATGTG ATTTTCTGCC AAGTTTGTCA GCTTGTGATG 
CGCAAGTTGT CTGAACTGAT TATCAACAAT GCCACTGAGG AACTTCTAAT TAAAGGTTTG 
AGTAAAGCCT GCTCCCTGCT CCCTGCTCCT GCTTCAACCA AGTGCCAGGA AGTGCTGGTA 
ACATTTGGCC CCTCTCTGTT GGACGTCCTC ATGCATGAGG TGAACCCGAA CTTTCTGTGC 
GGTGTGATCA GCCTCTGTTC TGCCAACCCG AATTTGGTGG GGACCCTTGA ACAACCTGCA 
GCAGCCATTG TATCTGCACT GCCCAAAGAG CCTGCACCGC CAAAACAGCC TGAAGAGCCC 
AAGCAGTCTG CATTGCGCGC CCATGTGCCT CCTCAGAAGA ACGGGGGGTT CTGTGAGGTG 
TGCAAGAAGC TGGTCATCTA TTTGGAACAT AACCTGGAGA AAAACAGCAC CAAGGAGGAG 
ATCCTGGCTG CACTTGAGAA GGGCTGCAGC TTCCTGCCAG ACCCTTACCA GAAGCAGTGT 
GATGAATTTG TGGCTGAGTA TGAGCCCTTA CTGCTGGAAA TCCTTGTGGA GGTGATGGAT 
CCTTCCTTTG TGTGCTCGAA AATTGGAGTC TGCCCTTCTG CCTATAAGCT GCTGCTGGGA 
ACCGAGAAGT GTGTCTGGGG CCCAGGCTAC TGGTGTCAGA ACATGGAGAC TGCTGCCCGA 
TGCAATGCTG TCGATCATTG CAAACGCCAT GTGTGGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177167.1
CDS	78..1739
Translation	MYALALLASLLVTALTSPVQDPKICSGGSAVVCRDVKTAVDCRAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATEEEILHYLEKTCAWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQRQLESNKIPEVDLARVVAPFMSNIPLLLYPQDRPRSQPQPKANEDVCQDCMKLVTDIQTAVRTNSSFVQGLVDHVKEDCDRLGPGVSDICKNYVDQYSEVAVQMMMHMQPKEICVMVGFCDEVKRVPMRTLVPATEAIKNILPALELTDPYEDVIQAQNVIFCQVCQLVMRKLSELIINNATEELLIKGLSKACSLLPAPASTKCQEVLVTFGPSLLDVLMHEVNPNFLCGVISLCSANPNLVGTLEQPAAAIVSALPKEPAPPKQPEEPKQSALRAHVPPQKNGGFCEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVEVMDPSFVCSKIGVCPSAYKLLLGTEKCVWGPGYWCQNMETAARCNAVDHCKRHVWN

Target ORF information:

RefSeq Version	NM_001190238.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prosaposin (Psap), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190238.1

ORF Insert Sequence:

ATGTATGCTC TCGCTCTCCT CGCCAGCCTT CTGGTCACCG CTCTGACCAG CCCTGTCCAG 
GACCCGAAGA TCTGCTCTGG GGGCTCAGCA GTAGTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTAGGG CTGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCAAAATCC 
CTTCCTTGTG ACATATGCAA AACGGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACTGAGG AGGAGATCCT CCATTACCTG GAGAAGACCT GTGCGTGGAT TCATGACTCC 
AGCCTGTCAG CCTCTTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGGG AGATGAGCAA CCCCGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AGACAGCTGG AGTCCAACAA GATCCCGGAG 
GTGGACCTGG CCCGCGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCGGC CTCGCAGCCA GCCGCAGCCC AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGTTGG TGACTGACAT CCAGACTGCT GTGAGGACCA ACTCCAGCTT TGTCCAGGGC 
TTGGTGGACC ACGTCAAGGA GGATTGTGAC CGCCTCGGGC CAGGCGTGTC TGACATATGC 
AAGAACTATG TTGACCAGTA TTCTGAGGTC GCCGTCCAGA TGATGATGCA CATGCAACCC 
AAGGAAATCT GTGTGATGGT TGGCTTCTGT GATGAGGTCA AGAGAGTGCC AATGAGGACT 
CTGGTCCCCG CCACTGAGGC CATCAAGAAT ATCCTCCCTG CCCTGGAGCT GACGGACCCC 
TATGAGGATG TGATCCAGGC CCAAAATGTG ATTTTCTGCC AAGTTTGTCA GCTTGTGATG 
CGCAAGTTGT CTGAACTGAT TATCAACAAT GCCACTGAGG AACTTCTAAT TAAAGGTTTG 
AGTAAAGCCT GCTCCCTGCT CCCTGCTCCT GCTTCAACCA AGTGCCAGGA AGTGCTGGTA 
ACATTTGGCC CCTCTCTGTT GGACGTCCTC ATGCATGAGG TGAACCCGAA CTTTCTGTGC 
GGTGTGATCA GCCTCTGTTC TGCCAACCCG AATTTGGTGG GGACCCTTGA ACAACCTGCA 
GCAGCCATTG TATCTGCACT GCCCAAAGAG CCTGCACCGC CAAAACAGCC TGAAGAGCCC 
AAGCAGTCTG CATTGCGCGC CCATGTGCCT CCTCAGAAGA ACGGGGGGTT CTGTGAGGTG 
TGCAAGAAGC TGGTCATCTA TTTGGAACAT AACCTGGAGA AAAACAGCAC CAAGGAGGAG 
ATCCTGGCTG CACTTGAGAA GGGCTGCAGC TTCCTGCCAG ACCCTTACCA GAAGCAGTGT 
GATGAATTTG TGGCTGAGTA TGAGCCCTTA CTGCTGGAAA TCCTTGTGGA GGTGATGGAT 
CCTTCCTTTG TGTGCTCGAA AATTGGAGTC TGCCCTTCTG CCTATAAGCT GCTGCTGGGA 
ACCGAGAAGT GTGTCTGGGG CCCAGGCTAC TGGTGTCAGA ACATGGAGAC TGCTGCCCGA 
TGCAATGCTG TCGATCATTG CAAACGCCAT GTGTGGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psap ( XM_006256403.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256465.1 Rattus norvegicus prosaposin (Psap), transcript variant X1, mRNA.

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CloneID	ORa39432
Clone ID Related Accession (Same CDS sequence)	XM_006256403.1
Accession Version	XM_006256403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	prosaposin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGCTC TCGCTCTCCT CGCCAGCCTT CTGGTCACCG CTCTGACCAG CCCTGTCCAG 
GACCCGAAGA TCTGCTCTGG GGGCTCAGCA GTAGTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTAGGG CTGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCAAAATCC 
CTTCCTTGTG ACATATGCAA AACGGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACTGAGG AGGAGATCCT CCATTACCTG GAGAAGACCT GTGCGTGGAT TCATGACTCC 
AGCCTGTCAG CCTCTTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGGG AGATGAGCAA CCCCGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AGACAGCTGG AGTCCAACAA GATCCCGGAG 
GTGGACCTGG CCCGCGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCGGC CTCGCAGCCA GCCGCAGCCC AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGTTGG TGACTGACAT CCAGACTGCT GTGAGGACCA ACTCCAGCTT TGTCCAGGGC 
TTGGTGGACC ACGTCAAGGA GGATTGTGAC CGCCTCGGGC CAGGCGTGTC TGACATATGC 
AAGAACTATG TTGACCAGTA TTCTGAGGTC GCCGTCCAGA TGATGATGCA CATGGATCAG 
CAACCCAAGG AAATCTGTGT GATGGTTGGC TTCTGTGATG AGGTCAAGAG AGTGCCAATG 
AGGACTCTGG TCCCCGCCAC TGAGGCCATC AAGAATATCC TCCCTGCCCT GGAGCTGACG 
GACCCCTATG AGCAGGATGT GATCCAGGCC CAAAATGTGA TTTTCTGCCA AGTTTGTCAG 
CTTGTGATGC GCAAGTTGTC TGAACTGATT ATCAACAATG CCACTGAGGA ACTTCTAATT 
AAAGGTTTGA GTAAAGCCTG CTCCCTGCTC CCTGCTCCTG CTTCAACCAA GTGCCAGGAA 
GTGCTGGTAA CATTTGGCCC CTCTCTGTTG GACGTCCTCA TGCATGAGGT GAACCCGAAC 
TTTCTGTGCG GTGTGATCAG CCTCTGTTCT GCCAACCCGA ATTTGGTGGG GACCCTTGAA 
CAACCTGCAG CAGCCATTGT ATCTGCACTG CCCAAAGAGC CTGCACCGCC AAAACAGCCT 
GAAGAGCCCA AGCAGTCTGC ATTGCGCGCC CATGTGCCTC CTCAGAAGAA CGGGGGGTTC 
TGTGAGGTGT GCAAGAAGCT GGTCATCTAT TTGGAACATA ACCTGGAGAA AAACAGCACC 
AAGGAGGAGA TCCTGGCTGC ACTTGAGAAG GGCTGCAGCT TCCTGCCAGA CCCTTACCAG 
AAGCAGTGTG ATGAATTTGT GGCTGAGTAT GAGCCCTTAC TGCTGGAAAT CCTTGTGGAG 
GTGATGGATC CTTCCTTTGT GTGCTCGAAA ATTGGAGTCT GCCCTTCTGC CTATAAGCTG 
CTGCTGGGAA CCGAGAAGTG TGTCTGGGGC CCAGGCTACT GGTGTCAGAA CATGGAGACT 
GCTGCCCGAT GCAATGCTGT CGATCATTGC AAACGCCATG TGTGGAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256465.1
CDS	90..1760
Translation	MYALALLASLLVTALTSPVQDPKICSGGSAVVCRDVKTAVDCRAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATEEEILHYLEKTCAWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQRQLESNKIPEVDLARVVAPFMSNIPLLLYPQDRPRSQPQPKANEDVCQDCMKLVTDIQTAVRTNSSFVQGLVDHVKEDCDRLGPGVSDICKNYVDQYSEVAVQMMMHMDQQPKEICVMVGFCDEVKRVPMRTLVPATEAIKNILPALELTDPYEQDVIQAQNVIFCQVCQLVMRKLSELIINNATEELLIKGLSKACSLLPAPASTKCQEVLVTFGPSLLDVLMHEVNPNFLCGVISLCSANPNLVGTLEQPAAAIVSALPKEPAPPKQPEEPKQSALRAHVPPQKNGGFCEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVEVMDPSFVCSKIGVCPSAYKLLLGTEKCVWGPGYWCQNMETAARCNAVDHCKRHVWN

Target ORF information:

RefSeq Version	XM_006256403.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prosaposin (Psap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256403.1

ORF Insert Sequence:

ATGTATGCTC TCGCTCTCCT CGCCAGCCTT CTGGTCACCG CTCTGACCAG CCCTGTCCAG 
GACCCGAAGA TCTGCTCTGG GGGCTCAGCA GTAGTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTAGGG CTGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCAAAATCC 
CTTCCTTGTG ACATATGCAA AACGGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACTGAGG AGGAGATCCT CCATTACCTG GAGAAGACCT GTGCGTGGAT TCATGACTCC 
AGCCTGTCAG CCTCTTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGGG AGATGAGCAA CCCCGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AGACAGCTGG AGTCCAACAA GATCCCGGAG 
GTGGACCTGG CCCGCGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCGGC CTCGCAGCCA GCCGCAGCCC AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGTTGG TGACTGACAT CCAGACTGCT GTGAGGACCA ACTCCAGCTT TGTCCAGGGC 
TTGGTGGACC ACGTCAAGGA GGATTGTGAC CGCCTCGGGC CAGGCGTGTC TGACATATGC 
AAGAACTATG TTGACCAGTA TTCTGAGGTC GCCGTCCAGA TGATGATGCA CATGGATCAG 
CAACCCAAGG AAATCTGTGT GATGGTTGGC TTCTGTGATG AGGTCAAGAG AGTGCCAATG 
AGGACTCTGG TCCCCGCCAC TGAGGCCATC AAGAATATCC TCCCTGCCCT GGAGCTGACG 
GACCCCTATG AGCAGGATGT GATCCAGGCC CAAAATGTGA TTTTCTGCCA AGTTTGTCAG 
CTTGTGATGC GCAAGTTGTC TGAACTGATT ATCAACAATG CCACTGAGGA ACTTCTAATT 
AAAGGTTTGA GTAAAGCCTG CTCCCTGCTC CCTGCTCCTG CTTCAACCAA GTGCCAGGAA 
GTGCTGGTAA CATTTGGCCC CTCTCTGTTG GACGTCCTCA TGCATGAGGT GAACCCGAAC 
TTTCTGTGCG GTGTGATCAG CCTCTGTTCT GCCAACCCGA ATTTGGTGGG GACCCTTGAA 
CAACCTGCAG CAGCCATTGT ATCTGCACTG CCCAAAGAGC CTGCACCGCC AAAACAGCCT 
GAAGAGCCCA AGCAGTCTGC ATTGCGCGCC CATGTGCCTC CTCAGAAGAA CGGGGGGTTC 
TGTGAGGTGT GCAAGAAGCT GGTCATCTAT TTGGAACATA ACCTGGAGAA AAACAGCACC 
AAGGAGGAGA TCCTGGCTGC ACTTGAGAAG GGCTGCAGCT TCCTGCCAGA CCCTTACCAG 
AAGCAGTGTG ATGAATTTGT GGCTGAGTAT GAGCCCTTAC TGCTGGAAAT CCTTGTGGAG 
GTGATGGATC CTTCCTTTGT GTGCTCGAAA ATTGGAGTCT GCCCTTCTGC CTATAAGCTG 
CTGCTGGGAA CCGAGAAGTG TGTCTGGGGC CCAGGCTACT GGTGTCAGAA CATGGAGACT 
GCTGCCCGAT GCAATGCTGT CGATCATTGC AAACGCCATG TGTGGAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prosaposin and its receptors are differentially expressed in the salivary glands of male and female rats.
Cell and tissue research373(2)439-457(2018 08)
Islam F,Khan MSI,Nabeka H,Saito S,Li X,Shimokawa T,Yamamiya K,Kobayashi N,Matsuda S

Lysosomal processing of progranulin.
Molecular neurodegeneration12(1)62(2017 08)
Zhou X,Paushter DH,Feng T,Sun L,Reinheckel T,Hu F

Impaired prosaposin lysosomal trafficking in frontotemporal lobar degeneration due to progranulin mutations.
Nature communications815277(2017 05)
Zhou X,Sun L,Bracko O,Choi JW,Jia Y,Nana AL,Brady OA,Hernandez JCC,Nishimura N,Seeley WW,Hu F

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

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Psap cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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