Nedd8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nedd8
Entrez Gene ID	25490
Full Name	neural precursor cell expressed, developmentally down-regulated 8
Synonyms	CDK8
General protein information	Preferred Names neural precursor cell expressed, developmentally down-regulated 8 Names NEDD8 neddylin neural precursor cell expressed, developmentally down-regulated gene 8 ubiquitin-like protein Nedd8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p13
Summary	displays differential expression after induction by nerve growth factor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138878.2	NP_620233.1	NEDD8 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NEDD8	XP_537389.2
Rattus norvegicus (Norway rat)	Nedd8	NP_620233.1
Drosophila melanogaster (fruit fly)	Nedd8	NP_609919.1
Saccharomyces cerevisiae (baker's yeast)	RUB1	NP_010423.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007913	XP_317573.3
Schizosaccharomyces pombe (fission yeast)	ned8	NP_595955.1
Magnaporthe oryzae (rice blast fungus)	MGG_09887	XP_003709980.1
Macaca mulatta (Rhesus monkey)	NEDD8	XP_001113390.1
Mus musculus (house mouse)	Nedd8	NP_032709.1
Kluyveromyces lactis	KLLA0F17974g	XP_455886.1
Eremothecium gossypii	AGOS_AGL185W	NP_986482.2
Homo sapiens (human)	NEDD8	NP_006147.1
Caenorhabditis elegans (roundworm)	ned-8	NP_492717.1
Pan troglodytes (chimpanzee)	NEDD8	XP_003952499.1
Bos taurus (cattle)	NEDD8	NP_777189.1
Danio rerio (zebrafish)	nedd8l	NP_001002557.1
Danio rerio (zebrafish)	nedd8	NP_958478.2
Xenopus tropicalis (tropical clawed frog)	nedd8	NP_001016973.1
Neurospora crassa	NCU03309	XP_964486.1

Gene Ontology
Bibliography

Related articles in PubMed

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

Expression of VACM-1/cul5 mutant in endothelial cells induces MAPK phosphorylation and maspin degradation and converts cells to the angiogenic phenotype.
Buchwalter A, Van Dort C, Schultz S, Smith R, Le IP, Abbott JL, Oosterhouse E, Johnson AE, Hansen-Smith F, Burnatowska-Hledin M
Microvascular research75(2)155-68(2008 Mar)

Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1.
Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA
Molecular cell17(3)341-50(2005 Feb)

Cullin-based ubiquitin ligase and its control by NEDD8-conjugating system.
Chiba T, Tanaka K
Current protein & peptide science5(3)177-84(2004 Jun)

APP-BP1 mediates APP-induced apoptosis and DNA synthesis and is increased in Alzheimer's disease brain.
Chen Y, Liu W, McPhie DL, Hassinger L, Neve RL
The Journal of cell biology163(1)27-33(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nedd8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nedd8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01849	NM_138878.2 Latest version!	Rattus norvegicus NEDD8 ubiquitin like modifier (Nedd8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nedd8 ( NM_138878.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620233.1 Rattus norvegicus NEDD8 ubiquitin like modifier (Nedd8), mRNA.

Price & Availability↑

CloneID	ORa01849
Clone ID Related Accession (Same CDS sequence)	NM_138878.2
Accession Version	NM_138878.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Rattus norvegicus(Norway rat)
Product	NEDD8 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB581170.1 and BC084728.1. On Oct 20, 2007 this sequence version replaced NM_138878.1. ##Evidence-Data-START## Transcript exon combination :: BC084728.1, CB576942.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTAATTA AAGTGAAGAC GCTGACTGGA AAGGAGATTG AGATTGACAT CGAACCCACA 
GACAAGGTGG AACGAATCAA GGAGCGTGTG GAAGAAAAAG AAGGAATTCC CCCACAACAG 
CAGCGGCTCA TCTACAGTGG CAAACAAATG AATGATGAGA AGACAGCAGC TGATTACAAG 
ATTCTAGGTG GTTCCGTCCT CCACCTGGTG TTGGCTCTTA GAGGAGGTGG TGGTCTTGGG 
CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620233.1
CDS	260..505
Translation	MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGGLGQ

Target ORF information:

RefSeq Version	NM_138878.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NEDD8 ubiquitin like modifier (Nedd8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138878.2

ORF Insert Sequence:

ATGCTAATTA AAGTGAAGAC GCTGACTGGA AAGGAGATTG AGATTGACAT CGAACCCACA 
GACAAGGTGG AACGAATCAA GGAGCGTGTG GAAGAAAAAG AAGGAATTCC CCCACAACAG 
CAGCGGCTCA TCTACAGTGG CAAACAAATG AATGATGAGA AGACAGCAGC TGATTACAAG 
ATTCTAGGTG GTTCCGTCCT CCACCTGGTG TTGGCTCTTA GAGGAGGTGG TGGTCTTGGG 
CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

Expression of VACM-1/cul5 mutant in endothelial cells induces MAPK phosphorylation and maspin degradation and converts cells to the angiogenic phenotype.
Microvascular research75(2)155-68(2008 Mar)
Buchwalter A,Van Dort C,Schultz S,Smith R,Le IP,Abbott JL,Oosterhouse E,Johnson AE,Hansen-Smith F,Burnatowska-Hledin M

Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1.
Molecular cell17(3)341-50(2005 Feb)
Huang DT,Paydar A,Zhuang M,Waddell MB,Holton JM,Schulman BA

Cullin-based ubiquitin ligase and its control by NEDD8-conjugating system.
Current protein & peptide science5(3)177-84(2004 Jun)
Chiba T,Tanaka K

APP-BP1 mediates APP-induced apoptosis and DNA synthesis and is increased in Alzheimer's disease brain.
The Journal of cell biology163(1)27-33(2003 Oct)
Chen Y,Liu W,McPhie DL,Hassinger L,Neve RL

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Nedd8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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