Cnp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cnp
Entrez Gene ID	25275
Full Name	2',3'-cyclic nucleotide 3' phosphodiesterase
Synonyms	CNPF,CNPI,CNPII,Cnp1
General protein information	Preferred Names 2',3'-cyclic nucleotide 3' phosphodiesterase Names 2',3'-cyclic-nucleotide 3'-phosphodiesterase CNPase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q31
Summary	microtubule-binding protein that promotes microtubule assembly and may regulate cellular distribution of microtubules [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006247260.3	XP_006247322.1	2',3'-cyclic-nucleotide 3'-phosphodiesterase isoform X1
XM_006247261.3	XP_006247323.1	2',3'-cyclic-nucleotide 3'-phosphodiesterase isoform X1
NM_012809.2	NP_036941.1	2',3'-cyclic-nucleotide 3'-phosphodiesterase

Pathways from BioSystems

Homologies

Homo sapiens (human)	CNP	NP_149124.3
Pan troglodytes (chimpanzee)	CNP	XP_511496.3
Mus musculus (house mouse)	Cnp	NP_034053.2
Gallus gallus (chicken)	CNP	NP_990381.1
Macaca mulatta (Rhesus monkey)	CNP	NP_001030605.1
Xenopus tropicalis (tropical clawed frog)	cnp	XP_004918763.1
Bos taurus (cattle)	CNP	NP_851336.1
Rattus norvegicus (Norway rat)	Cnp	NP_036941.1
Canis lupus familiaris (dog)	CNP	XP_005624533.1

Gene Ontology
Bibliography

Related articles in PubMed

Possible Involvement of 2',3'-Cyclic Nucleotide-3'-Phosphodiesterase in the Protein Phosphorylation-Mediated Regulation of the Permeability Transition Pore.
Baburina Y, Odinokova I, Azarashvili T, Akatov V, Sotnikova L, Krestinina O
International journal of molecular sciences19(11)(2018 Nov)

The cystine-glutamate exchanger (xCT, Slc7a11) is expressed in significant concentrations in a subpopulation of astrocytes in the mouse brain.
Ottestad-Hansen S, Hu QX, Follin-Arbelet VV, Bentea E, Sato H, Massie A, Zhou Y, Danbolt NC
Glia66(5)951-970(2018 05)

Effects of gene knockdown of CNP on ventricular remodeling after myocardial ischemia-reperfusion injury through NPRB/Cgmp signaling pathway in rats.
Wu LH, Zhang Q, Zhang S, Meng LY, Wang YC, Sheng CJ
Journal of cellular biochemistry119(2)1804-1818(2018 02)

The balance between cathepsin C and cystatin F controls remyelination in the brain of Plp1-overexpressing mouse, a chronic demyelinating disease model.
Shimizu T, Wisessmith W, Li J, Abe M, Sakimura K, Chetsawang B, Sahara Y, Tohyama K, Tanaka KF, Ikenaka K
Glia65(6)917-930(2017 06)

Perinatal methylmercury exposure perturbs the expression of Plp1 and Cnp splice variants in cerebellum of rat pups.
Padhi BK, Rosales M, Pelletier G
Neurotoxicology48223-30(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cnp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cnp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00998	NM_012809.2 Latest version!	Rattus norvegicus 2',3'-cyclic nucleotide 3' phosphodiesterase (Cnp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa33650	XM_006247261.3 Latest version!	Rattus norvegicus 2',3'-cyclic nucleotide 3' phosphodiesterase (Cnp), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa33650	XM_006247260.3 Latest version!	Rattus norvegicus 2',3'-cyclic nucleotide 3' phosphodiesterase (Cnp), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cnp ( NM_012809.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036941.1 Rattus norvegicus 2',3'-cyclic nucleotide 3' phosphodiesterase (Cnp), mRNA.

Price & Availability↑

CloneID	ORa00998
Clone ID Related Accession (Same CDS sequence)	NM_012809.2
Accession Version	NM_012809.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Rattus norvegicus(Norway rat)
Product	2',3'-cyclic-nucleotide 3'-phosphodiesterase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC098066.1. On Aug 18, 2012 this sequence version replaced NM_012809.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098066.1, L16532.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGCACAA GCTTTGCCCG AAAAAGCCAC ACATTCCTGC CCAAGATCTT CTTCAGAAAA 
ATGTCATCCT CAGGAGCTAA GGACAAGCCA GAGCTGCAGT TCCCTTTCCT TCAAGATGAG 
GACACGGTGG CTACGCTGCA CGAGTGCAAG ACGCTGTTCA TCCTGCGTGG CCTGCCGGGC 
AGCGGCAAGT CCACTCTGGC TCGGCTCATC GTGGAGAAGT ACCACAACGG CACCAAGATG 
GTGTCTGCTG ATGCTTACAA GATCATTCCT GGCTCTCGGG CAGACTTCTC CGAGGAGTAC 
AAGCGTCTGG ACGAGGACCT GGCTGGCTAC TGCCGCCGGG ACATCAGGGT TCTTGTGCTC 
GATGACACCA ACCATGAGCG GGAACGGCTG GACCAGCTTT TTGAAATGGC AGACCAGTAC 
CAGTACCAGG TGGTGCTGGT GGAGCCCAAG ACGGCGTGGC GACTAGACTG TGCCCAGCTC 
AAGGAGAAGA ACCAATGGCA GCTGTCGCTC GATGACCTGA AGAAGCTGAA GCCCGGGCTG 
GAGAAGGACT TCCTGCCACT TTACTTTGGC TGGTTCCTGA CCAAAAAGAG TTCCGAGACC 
CTCCGAAAAG CCGGCCAGGT CTTTCTGGAG GAGCTGGGAA ATCACAAGGC TTTCAAGAAA 
GAGCTTCGAC ACTTCATTTC TGGGGATGAA CCCAAGGAGA AGCTTGACCT GGTCAGCTAT 
TTTGGCAAGA GACCTCCAGG CGTGCTGCAC TGTACAACCA AATTCTGTGA CTACGGGAAG 
GCCACCGGGG CAGAAGAATA TGCCCAACAG GATGTGGTGA GGAGATCTTA CGGCAAGGCC 
TTCAAACTGT CCATCTCTGC CCTCTTTGTG ACACCCAAGA CAGCTGGGGC CCAGGTGGTG 
CTTAATGAGC AGGAGCTGCA GTTGTGGCCA AGTGACCTGG ACAAGCCCTC ATCCTCCGAG 
AGCCTGCCCC CAGGGAGCCG AGCTCACGTC ACCCTAGGCT GTGCAGCCGA CGTGCAGCCA 
GTGCAGACAG GCCTTGACCT TTTAGAGATT TTACAGCAGG TGAAGGGGGG CAGCCAAGGT 
GAGGAGGTGG GTGAGCTCCC CCGGGGCAAG CTCTATTCCT TGGGCAAAGG GCGTTGGATG 
CTGAGCTTGG CGAAGAAGAT GGAAGTCAAG GCCATCTTCA CGGGGTACTA TGGGAAGGGC 
AAACCTGTAC CCGTACACGG CAGCCGGAAG GGGGGTGCCA TGCAGATCTG CACCATCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036941.1
CDS	81..1343
Translation	MSTSFARKSHTFLPKIFFRKMSSSGAKDKPELQFPFLQDEDTVATLHECKTLFILRGLPGSGKSTLARLIVEKYHNGTKMVSADAYKIIPGSRADFSEEYKRLDEDLAGYCRRDIRVLVLDDTNHERERLDQLFEMADQYQYQVVLVEPKTAWRLDCAQLKEKNQWQLSLDDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRHFISGDEPKEKLDLVSYFGKRPPGVLHCTTKFCDYGKATGAEEYAQQDVVRRSYGKAFKLSISALFVTPKTAGAQVVLNEQELQLWPSDLDKPSSSESLPPGSRAHVTLGCAADVQPVQTGLDLLEILQQVKGGSQGEEVGELPRGKLYSLGKGRWMLSLAKKMEVKAIFTGYYGKGKPVPVHGSRKGGAMQICTII

Target ORF information:

RefSeq Version	NM_012809.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 2',3'-cyclic nucleotide 3' phosphodiesterase (Cnp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012809.2

ORF Insert Sequence:

ATGAGCACAA GCTTTGCCCG AAAAAGCCAC ACATTCCTGC CCAAGATCTT CTTCAGAAAA 
ATGTCATCCT CAGGAGCTAA GGACAAGCCA GAGCTGCAGT TCCCTTTCCT TCAAGATGAG 
GACACGGTGG CTACGCTGCA CGAGTGCAAG ACGCTGTTCA TCCTGCGTGG CCTGCCGGGC 
AGCGGCAAGT CCACTCTGGC TCGGCTCATC GTGGAGAAGT ACCACAACGG CACCAAGATG 
GTGTCTGCTG ATGCTTACAA GATCATTCCT GGCTCTCGGG CAGACTTCTC CGAGGAGTAC 
AAGCGTCTGG ACGAGGACCT GGCTGGCTAC TGCCGCCGGG ACATCAGGGT TCTTGTGCTC 
GATGACACCA ACCATGAGCG GGAACGGCTG GACCAGCTTT TTGAAATGGC AGACCAGTAC 
CAGTACCAGG TGGTGCTGGT GGAGCCCAAG ACGGCGTGGC GACTAGACTG TGCCCAGCTC 
AAGGAGAAGA ACCAATGGCA GCTGTCGCTC GATGACCTGA AGAAGCTGAA GCCCGGGCTG 
GAGAAGGACT TCCTGCCACT TTACTTTGGC TGGTTCCTGA CCAAAAAGAG TTCCGAGACC 
CTCCGAAAAG CCGGCCAGGT CTTTCTGGAG GAGCTGGGAA ATCACAAGGC TTTCAAGAAA 
GAGCTTCGAC ACTTCATTTC TGGGGATGAA CCCAAGGAGA AGCTTGACCT GGTCAGCTAT 
TTTGGCAAGA GACCTCCAGG CGTGCTGCAC TGTACAACCA AATTCTGTGA CTACGGGAAG 
GCCACCGGGG CAGAAGAATA TGCCCAACAG GATGTGGTGA GGAGATCTTA CGGCAAGGCC 
TTCAAACTGT CCATCTCTGC CCTCTTTGTG ACACCCAAGA CAGCTGGGGC CCAGGTGGTG 
CTTAATGAGC AGGAGCTGCA GTTGTGGCCA AGTGACCTGG ACAAGCCCTC ATCCTCCGAG 
AGCCTGCCCC CAGGGAGCCG AGCTCACGTC ACCCTAGGCT GTGCAGCCGA CGTGCAGCCA 
GTGCAGACAG GCCTTGACCT TTTAGAGATT TTACAGCAGG TGAAGGGGGG CAGCCAAGGT 
GAGGAGGTGG GTGAGCTCCC CCGGGGCAAG CTCTATTCCT TGGGCAAAGG GCGTTGGATG 
CTGAGCTTGG CGAAGAAGAT GGAAGTCAAG GCCATCTTCA CGGGGTACTA TGGGAAGGGC 
AAACCTGTAC CCGTACACGG CAGCCGGAAG GGGGGTGCCA TGCAGATCTG CACCATCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cnp ( XM_006247260.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247322.1 Rattus norvegicus 2',3'-cyclic nucleotide 3' phosphodiesterase (Cnp), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33650
Clone ID Related Accession (Same CDS sequence)	XM_006247260.3 , XM_006247261.3
Accession Version	XM_006247260.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	2',3'-cyclic-nucleotide 3'-phosphodiesterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247260.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCCT CAGGAGCTAA GGACAAGCCA GAGCTGCAGT TCCCTTTCCT TCAAGATGAG 
GACACGGTGG CTACGCTGCA CGAGTGCAAG ACGCTGTTCA TCCTGCGTGG CCTGCCGGGC 
AGCGGCAAGT CCACTCTGGC TCGGCTCATC GTGGAGAAGT ACCACAACGG CACCAAGATG 
GTGTCTGCTG ATGCTTACAA GATCATTCCT GGCTCTCGGG CAGACTTCTC CGAGGAGTAC 
AAGCGTCTGG ACGAGGACCT GGCTGGCTAC TGCCGCCGGG ACATCAGGGT TCTTGTGCTC 
GATGACACCA ACCATGAGCG GGAACGGCTG GACCAGCTTT TTGAAATGGC AGACCAGTAC 
CAGTACCAGG TGGTGCTGGT GGAGCCCAAG ACGGCGTGGC GACTAGACTG TGCCCAGCTC 
AAGGAGAAGA ACCAATGGCA GCTGTCGCTC GATGACCTGA AGAAGCTGAA GCCCGGGCTG 
GAGAAGGACT TCCTGCCACT TTACTTTGGC TGGTTCCTGA CCAAAAAGAG TTCCGAGACC 
CTCCGAAAAG CCGGCCAGGT CTTTCTGGAG GAGCTGGGAA ATCACAAGGC TTTCAAGAAA 
GAGCTTCGAC ACTTCATTTC TGGGGATGAA CCCAAGGAGA AGCTTGACCT GGTCAGCTAT 
TTTGGCAAGA GACCTCCAGG CGTGCTGCAC TGTACAACCA AATTCTGTGA CTACGGGAAG 
GCCACCGGGG CAGAAGAATA TGCCCAACAG GATGTGGTGA GGAGATCTTA CGGCAAGGCC 
TTCAAACTGT CCATCTCTGC CCTCTTTGTG ACACCCAAGA CAGCTGGGGC CCAGGTGGTG 
CTTAATGAGC AGGAGCTGCA GTTGTGGCCA AGTGACCTGG ACAAGCCCTC ATCCTCCGAG 
AGCCTGCCCC CAGGGAGCCG AGCTCACGTC ACCCTAGGCT GTGCAGCCGA CGTGCAGCCA 
GTGCAGACAG GCCTTGACCT TTTAGAGATT TTACAGCAGG TGAAGGGGGG CAGCCAAGGT 
GAGGAGGTGG GTGAGCTCCC CCGGGGCAAG CTCTATTCCT TGGGCAAAGG GCGTTGGATG 
CTGAGCTTGG CGAAGAAGAT GGAAGTCAAG GCCATCTTCA CGGGGTACTA TGGGAAGGGC 
AAACCTGTAC CCGTACACGG CAGCCGGAAG GGGGGTGCCA TGCAGATCTG CACCATCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247322.1
CDS	167..1369
Translation	MSSSGAKDKPELQFPFLQDEDTVATLHECKTLFILRGLPGSGKSTLARLIVEKYHNGTKMVSADAYKIIPGSRADFSEEYKRLDEDLAGYCRRDIRVLVLDDTNHERERLDQLFEMADQYQYQVVLVEPKTAWRLDCAQLKEKNQWQLSLDDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRHFISGDEPKEKLDLVSYFGKRPPGVLHCTTKFCDYGKATGAEEYAQQDVVRRSYGKAFKLSISALFVTPKTAGAQVVLNEQELQLWPSDLDKPSSSESLPPGSRAHVTLGCAADVQPVQTGLDLLEILQQVKGGSQGEEVGELPRGKLYSLGKGRWMLSLAKKMEVKAIFTGYYGKGKPVPVHGSRKGGAMQICTII

Target ORF information:

RefSeq Version	XM_006247260.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 2',3'-cyclic nucleotide 3' phosphodiesterase (Cnp), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247260.3

ORF Insert Sequence:

ATGTCATCCT CAGGAGCTAA GGACAAGCCA GAGCTGCAGT TCCCTTTCCT TCAAGATGAG 
GACACGGTGG CTACGCTGCA CGAGTGCAAG ACGCTGTTCA TCCTGCGTGG CCTGCCGGGC 
AGCGGCAAGT CCACTCTGGC TCGGCTCATC GTGGAGAAGT ACCACAACGG CACCAAGATG 
GTGTCTGCTG ATGCTTACAA GATCATTCCT GGCTCTCGGG CAGACTTCTC CGAGGAGTAC 
AAGCGTCTGG ACGAGGACCT GGCTGGCTAC TGCCGCCGGG ACATCAGGGT TCTTGTGCTC 
GATGACACCA ACCATGAGCG GGAACGGCTG GACCAGCTTT TTGAAATGGC AGACCAGTAC 
CAGTACCAGG TGGTGCTGGT GGAGCCCAAG ACGGCGTGGC GACTAGACTG TGCCCAGCTC 
AAGGAGAAGA ACCAATGGCA GCTGTCGCTC GATGACCTGA AGAAGCTGAA GCCCGGGCTG 
GAGAAGGACT TCCTGCCACT TTACTTTGGC TGGTTCCTGA CCAAAAAGAG TTCCGAGACC 
CTCCGAAAAG CCGGCCAGGT CTTTCTGGAG GAGCTGGGAA ATCACAAGGC TTTCAAGAAA 
GAGCTTCGAC ACTTCATTTC TGGGGATGAA CCCAAGGAGA AGCTTGACCT GGTCAGCTAT 
TTTGGCAAGA GACCTCCAGG CGTGCTGCAC TGTACAACCA AATTCTGTGA CTACGGGAAG 
GCCACCGGGG CAGAAGAATA TGCCCAACAG GATGTGGTGA GGAGATCTTA CGGCAAGGCC 
TTCAAACTGT CCATCTCTGC CCTCTTTGTG ACACCCAAGA CAGCTGGGGC CCAGGTGGTG 
CTTAATGAGC AGGAGCTGCA GTTGTGGCCA AGTGACCTGG ACAAGCCCTC ATCCTCCGAG 
AGCCTGCCCC CAGGGAGCCG AGCTCACGTC ACCCTAGGCT GTGCAGCCGA CGTGCAGCCA 
GTGCAGACAG GCCTTGACCT TTTAGAGATT TTACAGCAGG TGAAGGGGGG CAGCCAAGGT 
GAGGAGGTGG GTGAGCTCCC CCGGGGCAAG CTCTATTCCT TGGGCAAAGG GCGTTGGATG 
CTGAGCTTGG CGAAGAAGAT GGAAGTCAAG GCCATCTTCA CGGGGTACTA TGGGAAGGGC 
AAACCTGTAC CCGTACACGG CAGCCGGAAG GGGGGTGCCA TGCAGATCTG CACCATCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cnp ( XM_006247261.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247323.1 Rattus norvegicus 2',3'-cyclic nucleotide 3' phosphodiesterase (Cnp), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa33650
Clone ID Related Accession (Same CDS sequence)	XM_006247260.3 , XM_006247261.3
Accession Version	XM_006247261.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	2',3'-cyclic-nucleotide 3'-phosphodiesterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247261.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCCT CAGGAGCTAA GGACAAGCCA GAGCTGCAGT TCCCTTTCCT TCAAGATGAG 
GACACGGTGG CTACGCTGCA CGAGTGCAAG ACGCTGTTCA TCCTGCGTGG CCTGCCGGGC 
AGCGGCAAGT CCACTCTGGC TCGGCTCATC GTGGAGAAGT ACCACAACGG CACCAAGATG 
GTGTCTGCTG ATGCTTACAA GATCATTCCT GGCTCTCGGG CAGACTTCTC CGAGGAGTAC 
AAGCGTCTGG ACGAGGACCT GGCTGGCTAC TGCCGCCGGG ACATCAGGGT TCTTGTGCTC 
GATGACACCA ACCATGAGCG GGAACGGCTG GACCAGCTTT TTGAAATGGC AGACCAGTAC 
CAGTACCAGG TGGTGCTGGT GGAGCCCAAG ACGGCGTGGC GACTAGACTG TGCCCAGCTC 
AAGGAGAAGA ACCAATGGCA GCTGTCGCTC GATGACCTGA AGAAGCTGAA GCCCGGGCTG 
GAGAAGGACT TCCTGCCACT TTACTTTGGC TGGTTCCTGA CCAAAAAGAG TTCCGAGACC 
CTCCGAAAAG CCGGCCAGGT CTTTCTGGAG GAGCTGGGAA ATCACAAGGC TTTCAAGAAA 
GAGCTTCGAC ACTTCATTTC TGGGGATGAA CCCAAGGAGA AGCTTGACCT GGTCAGCTAT 
TTTGGCAAGA GACCTCCAGG CGTGCTGCAC TGTACAACCA AATTCTGTGA CTACGGGAAG 
GCCACCGGGG CAGAAGAATA TGCCCAACAG GATGTGGTGA GGAGATCTTA CGGCAAGGCC 
TTCAAACTGT CCATCTCTGC CCTCTTTGTG ACACCCAAGA CAGCTGGGGC CCAGGTGGTG 
CTTAATGAGC AGGAGCTGCA GTTGTGGCCA AGTGACCTGG ACAAGCCCTC ATCCTCCGAG 
AGCCTGCCCC CAGGGAGCCG AGCTCACGTC ACCCTAGGCT GTGCAGCCGA CGTGCAGCCA 
GTGCAGACAG GCCTTGACCT TTTAGAGATT TTACAGCAGG TGAAGGGGGG CAGCCAAGGT 
GAGGAGGTGG GTGAGCTCCC CCGGGGCAAG CTCTATTCCT TGGGCAAAGG GCGTTGGATG 
CTGAGCTTGG CGAAGAAGAT GGAAGTCAAG GCCATCTTCA CGGGGTACTA TGGGAAGGGC 
AAACCTGTAC CCGTACACGG CAGCCGGAAG GGGGGTGCCA TGCAGATCTG CACCATCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247323.1
CDS	155..1357
Translation	MSSSGAKDKPELQFPFLQDEDTVATLHECKTLFILRGLPGSGKSTLARLIVEKYHNGTKMVSADAYKIIPGSRADFSEEYKRLDEDLAGYCRRDIRVLVLDDTNHERERLDQLFEMADQYQYQVVLVEPKTAWRLDCAQLKEKNQWQLSLDDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRHFISGDEPKEKLDLVSYFGKRPPGVLHCTTKFCDYGKATGAEEYAQQDVVRRSYGKAFKLSISALFVTPKTAGAQVVLNEQELQLWPSDLDKPSSSESLPPGSRAHVTLGCAADVQPVQTGLDLLEILQQVKGGSQGEEVGELPRGKLYSLGKGRWMLSLAKKMEVKAIFTGYYGKGKPVPVHGSRKGGAMQICTII

Target ORF information:

RefSeq Version	XM_006247261.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 2',3'-cyclic nucleotide 3' phosphodiesterase (Cnp), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247261.3

ORF Insert Sequence:

ATGTCATCCT CAGGAGCTAA GGACAAGCCA GAGCTGCAGT TCCCTTTCCT TCAAGATGAG 
GACACGGTGG CTACGCTGCA CGAGTGCAAG ACGCTGTTCA TCCTGCGTGG CCTGCCGGGC 
AGCGGCAAGT CCACTCTGGC TCGGCTCATC GTGGAGAAGT ACCACAACGG CACCAAGATG 
GTGTCTGCTG ATGCTTACAA GATCATTCCT GGCTCTCGGG CAGACTTCTC CGAGGAGTAC 
AAGCGTCTGG ACGAGGACCT GGCTGGCTAC TGCCGCCGGG ACATCAGGGT TCTTGTGCTC 
GATGACACCA ACCATGAGCG GGAACGGCTG GACCAGCTTT TTGAAATGGC AGACCAGTAC 
CAGTACCAGG TGGTGCTGGT GGAGCCCAAG ACGGCGTGGC GACTAGACTG TGCCCAGCTC 
AAGGAGAAGA ACCAATGGCA GCTGTCGCTC GATGACCTGA AGAAGCTGAA GCCCGGGCTG 
GAGAAGGACT TCCTGCCACT TTACTTTGGC TGGTTCCTGA CCAAAAAGAG TTCCGAGACC 
CTCCGAAAAG CCGGCCAGGT CTTTCTGGAG GAGCTGGGAA ATCACAAGGC TTTCAAGAAA 
GAGCTTCGAC ACTTCATTTC TGGGGATGAA CCCAAGGAGA AGCTTGACCT GGTCAGCTAT 
TTTGGCAAGA GACCTCCAGG CGTGCTGCAC TGTACAACCA AATTCTGTGA CTACGGGAAG 
GCCACCGGGG CAGAAGAATA TGCCCAACAG GATGTGGTGA GGAGATCTTA CGGCAAGGCC 
TTCAAACTGT CCATCTCTGC CCTCTTTGTG ACACCCAAGA CAGCTGGGGC CCAGGTGGTG 
CTTAATGAGC AGGAGCTGCA GTTGTGGCCA AGTGACCTGG ACAAGCCCTC ATCCTCCGAG 
AGCCTGCCCC CAGGGAGCCG AGCTCACGTC ACCCTAGGCT GTGCAGCCGA CGTGCAGCCA 
GTGCAGACAG GCCTTGACCT TTTAGAGATT TTACAGCAGG TGAAGGGGGG CAGCCAAGGT 
GAGGAGGTGG GTGAGCTCCC CCGGGGCAAG CTCTATTCCT TGGGCAAAGG GCGTTGGATG 
CTGAGCTTGG CGAAGAAGAT GGAAGTCAAG GCCATCTTCA CGGGGTACTA TGGGAAGGGC 
AAACCTGTAC CCGTACACGG CAGCCGGAAG GGGGGTGCCA TGCAGATCTG CACCATCATC 
TGA