Bckdha cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bckdha
Entrez Gene ID	25244
Full Name	branched chain ketoacid dehydrogenase E1, alpha polypeptide
Synonyms	BCKDA,E1a
General protein information	Preferred Names branched chain ketoacid dehydrogenase E1, alpha polypeptide Names 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial BCKDE1A BCKDH E1-alpha branched chain alpha-ketoacid dehydrogenase subunit E1 alpha branched chain keto acid dehydrogenase subunit E1, alpha polypeptide branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	plays a role in the conversion of 3-methyl-2-oxobutanoate and lipoamide to S-(2-methylpropanoyl)dihydrolipoamide and CO2 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017588814.1	XP_017444303.1	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial isoform X1
NM_012782.1	NP_036914.1	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	BCKDHA	XP_001101959.2
Danio rerio (zebrafish)	bckdha	NP_001019590.1
Mus musculus (house mouse)	Bckdha	NP_031559.3
Pan troglodytes (chimpanzee)	BCKDHA	NP_001092034.1
Canis lupus familiaris (dog)	BCKDHA	XP_866392.1
Rattus norvegicus (Norway rat)	Bckdha	NP_036914.1
Drosophila melanogaster (fruit fly)	CG8199	NP_649905.1
Arabidopsis thaliana (thale cress)	AT5G09300	NP_568209.1
Neurospora crassa	NCU09864	XP_960181.2
Oryza sativa (rice)	Os12g0183100	NP_001066324.1
Magnaporthe oryzae (rice blast fungus)	MGG_03840	XP_003720051.1
Homo sapiens (human)	BCKDHA	NP_000700.1
Bos taurus (cattle)	BCKDHA	NP_776931.1
Caenorhabditis elegans (roundworm)	Y39E4A.3	NP_001033376.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003136	XP_312823.5

Gene Ontology
Bibliography

Related articles in PubMed

[Antiapoptotic gene bcl-2 prevents cellular senescence program reactivation induced by histone deacetylase inhibitor sodium butyrate in E1A and cHa-ras transformed rat fibroblasts].
Gordeev SA, Bykova TV, Zubova SG, Aksenov ND, Pospelova TV
Tsitologiia57(2)135-43(2015)

Simvastatin increases liver branched-chain α-ketoacid dehydrogenase activity in rats fed with low protein diet.
Knapik-Czajka M
Toxicology325107-14(2014 Nov)

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, León IR, Bak S, Mogensen M, Wrzesinski K, Højlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS.
Moser K, White FM
Journal of proteome research5(1)98-104(2006 Jan)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Bckdha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bckdha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12488	NM_012782.1 Latest version!	Rattus norvegicus branched chain keto acid dehydrogenase E1 subunit alpha (Bckdha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa56403	XM_017588814.1 Latest version!	Rattus norvegicus branched chain ketoacid dehydrogenase E1, alpha polypeptide (Bckdha), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bckdha ( NM_012782.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036914.1 Rattus norvegicus branched chain keto acid dehydrogenase E1 subunit alpha (Bckdha), mRNA.

Price & Availability↑

CloneID	ORa12488
Clone ID Related Accession (Same CDS sequence)	NM_012782.1
Accession Version	NM_012782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573660800000
Organism	Rattus norvegicus(Norway rat)
Product	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB579195.1, J02827.1 and BC089915.1. On Oct 17, 2005 this sequence version replaced XM_341805.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC161819.1, J02827.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGTAG CGATGTCCGC GGCCAAGATC TGGAGGCCGA GCCGTGGCCT GCGCCAGGCT 
GCCCTCTTGC TGTTGGGGCG ACCTGGGGCT CGGGGCTTGG CTAGATTCCA CCCCAGCAGG 
CAGCAGCAAC AGCAGTTCCC ATCCCTGGAT GACAAGCCCC AGTTCCCAGG GGCCTCTGCA 
GAGTTTGTAG ACAAGCTTGA GTTCATCCAG CCCAATGTCA TCTCCGGCAT CCCCATCTAC 
CGCGTCATGG ACCGACAGGG CCAGATCATC AACCCCAGTG AAGATCCCCA CCTGCCCCAG 
GAGGAGGTCC TGAAGTTGTA CAGGAGCATG ACGCTGCTCA ACACCATGGA CCGCATTCTC 
TATGAGTCCC AGCGACAGGG CCGGATCTCC TTCTACATGA CCAACTATGG CGAGGAGGGG 
ACACATGTGG GGAGTGCCGC TGCCCTGGAG CGAACGGACC TGGTGTTTGG CCAGTACCGG 
GAGGCAGGTG TGCTCATGTA CCGGGACTAC CCGCTGGAGC TGTTCATGGC CCAGTGCTAC 
GGCAACGTGA GTGACCCAGG CAAGGGACGC CAGATGCCCG TTCACTACGG CTGCAAGGAG 
CGTCACTTCG TCACCATTTC CTCTCCACTG GCTACGCAGA TCCCTCAGGC GGTAGGGGCA 
GCCTATGCTG CCAAGCGGGC CAATGCCAAT CAGATCGTGA TCTGTTACTT TGGCGAGGGG 
GCAGCCAGTG AAGGGGATGC CCACGCCGGT TTCAATTTTG CTGCCACCTT GGAGTGTCCT 
ATCATCTTCT TCTGCCGGAA CAATGGCTAT GCCATCTCCA CACCAACCTC TGAGCAGTAC 
CGTGGGGATG GCATAGCGGC TCGGGGCCCT GGGTATGGCA TCATGTCAAT CCGTGTGGAC 
GGCAACGATG TGTTTGCGGT GTACAATGCC ACTAAGGAGG CCCGACGGCG GGCTGTGGCT 
GAGAACCAGC CCTTCCTCAT TGAGGCCATG ACCTACAGGA TCGGGCACCA CAGCACCAGT 
GACGACAGCT CTGCGTACCG TTCGGTGGAT GAGGTCAATT ACTGGGACAA GCAGGACCAC 
CCGATCTCGA GACTGAGGCA GTACCTGCTG AACCAGGGTT GGTGGGATGA GGAACAGGAG 
AAGGCCTGGC GGAAGCAGTC ACGAAAGAAG GTGATGGAGG CCTTTGAACA GGCTGAGCGG 
AAGCTAAAGC CCAACCCAAG CCTCCTCTTC TCCGATGTGT ACCAGGAGAT GCCTGCCCAG 
CTGCGCAGGC AGCAGGAGTC CCTGGCTCGG CACCTGCAGA CCTACGGGGA GCACTACCCC 
CTGGACCACT TTGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036914.1
CDS	2..1342
Translation	MAVAMSAAKIWRPSRGLRQAALLLLGRPGARGLARFHPSRQQQQQFPSLDDKPQFPGASAEFVDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPQEEVLKLYRSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALERTDLVFGQYREAGVLMYRDYPLELFMAQCYGNVSDPGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANANQIVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHPISRLRQYLLNQGWWDEEQEKAWRKQSRKKVMEAFEQAERKLKPNPSLLFSDVYQEMPAQLRRQQESLARHLQTYGEHYPLDHFDK

Target ORF information:

RefSeq Version	NM_012782.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus branched chain keto acid dehydrogenase E1 subunit alpha (Bckdha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012782.1

ORF Insert Sequence:

ATGGCGGTAG CGATGTCCGC GGCCAAGATC TGGAGGCCGA GCCGTGGCCT GCGCCAGGCT 
GCCCTCTTGC TGTTGGGGCG ACCTGGGGCT CGGGGCTTGG CTAGATTCCA CCCCAGCAGG 
CAGCAGCAAC AGCAGTTCCC ATCCCTGGAT GACAAGCCCC AGTTCCCAGG GGCCTCTGCA 
GAGTTTGTAG ACAAGCTTGA GTTCATCCAG CCCAATGTCA TCTCCGGCAT CCCCATCTAC 
CGCGTCATGG ACCGACAGGG CCAGATCATC AACCCCAGTG AAGATCCCCA CCTGCCCCAG 
GAGGAGGTCC TGAAGTTGTA CAGGAGCATG ACGCTGCTCA ACACCATGGA CCGCATTCTC 
TATGAGTCCC AGCGACAGGG CCGGATCTCC TTCTACATGA CCAACTATGG CGAGGAGGGG 
ACACATGTGG GGAGTGCCGC TGCCCTGGAG CGAACGGACC TGGTGTTTGG CCAGTACCGG 
GAGGCAGGTG TGCTCATGTA CCGGGACTAC CCGCTGGAGC TGTTCATGGC CCAGTGCTAC 
GGCAACGTGA GTGACCCAGG CAAGGGACGC CAGATGCCCG TTCACTACGG CTGCAAGGAG 
CGTCACTTCG TCACCATTTC CTCTCCACTG GCTACGCAGA TCCCTCAGGC GGTAGGGGCA 
GCCTATGCTG CCAAGCGGGC CAATGCCAAT CAGATCGTGA TCTGTTACTT TGGCGAGGGG 
GCAGCCAGTG AAGGGGATGC CCACGCCGGT TTCAATTTTG CTGCCACCTT GGAGTGTCCT 
ATCATCTTCT TCTGCCGGAA CAATGGCTAT GCCATCTCCA CACCAACCTC TGAGCAGTAC 
CGTGGGGATG GCATAGCGGC TCGGGGCCCT GGGTATGGCA TCATGTCAAT CCGTGTGGAC 
GGCAACGATG TGTTTGCGGT GTACAATGCC ACTAAGGAGG CCCGACGGCG GGCTGTGGCT 
GAGAACCAGC CCTTCCTCAT TGAGGCCATG ACCTACAGGA TCGGGCACCA CAGCACCAGT 
GACGACAGCT CTGCGTACCG TTCGGTGGAT GAGGTCAATT ACTGGGACAA GCAGGACCAC 
CCGATCTCGA GACTGAGGCA GTACCTGCTG AACCAGGGTT GGTGGGATGA GGAACAGGAG 
AAGGCCTGGC GGAAGCAGTC ACGAAAGAAG GTGATGGAGG CCTTTGAACA GGCTGAGCGG 
AAGCTAAAGC CCAACCCAAG CCTCCTCTTC TCCGATGTGT ACCAGGAGAT GCCTGCCCAG 
CTGCGCAGGC AGCAGGAGTC CCTGGCTCGG CACCTGCAGA CCTACGGGGA GCACTACCCC 
CTGGACCACT TTGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bckdha ( XM_017588814.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444303.1 Rattus norvegicus branched chain ketoacid dehydrogenase E1, alpha polypeptide (Bckdha), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56403
Clone ID Related Accession (Same CDS sequence)	XM_017588814.1
Accession Version	XM_017588814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTAG CGATGTCCGC GGCCAAGATC TGGAGGCCGA GCCGTGGCCT GCGCCAGGCT 
GCCCTCTTGC TGTTGGGGCG ACCTGGGGCT CGGGGCTTGG CTAGATTCCT GCCCCAGGAG 
GAGGTCCTGA AGTTGTACAG GAGCATGACG CTGCTCAACA CCATGGACCG CATTCTCTAT 
GAGTCCCAGC GACAGGGCCG GATCTCCTTC TACATGACCA ACTATGGCGA GGAGGGGACA 
CATGTGGGGA GTGCCGCTGC CCTGGAGCGA ACGGACCTGG TGTTTGGCCA GTACCGGGAG 
GCAGGTGTGC TCATGTACCG GGACTACCCG CTGGAGCTGT TCATGGCCCA GTGCTACGGC 
AACGTGAGTG ACCCAGGCAA GGGACGCCAG ATGCCCGTTC ACTACGGCTG CAAGGAGCGT 
CACTTCGTCA CCATTTCCTC TCCACTGGCT ACGCAGATCC CTCAGGCGGT AGGGGCAGCC 
TATGCTGCCA AGCGGGCCAA TGCCAATCAG ATCGTGATCT GTTACTTTGG CGAGGGGGCA 
GCCAGTGAAG GGGATGCCCA CGCCGGTTTC AATTTTGCTG CCACCTTGGA GTGTCCTATC 
ATCTTCTTCT GCCGGAACAA TGGCTATGCC ATCTCCACAC CAACCTCTGA GCAGTACCGT 
GGGGATGGCA TAGCGGCTCG GGGCCCTGGG TATGGCATCA TGTCAATCCG TGTGGACGGC 
AACGATGTGT TTGCGGTGTA CAATGCCACT AAGGAGGCCC GACGGCGGGC TGTGGCTGAG 
AACCAGCCCT TCCTCATTGA GGCCATGACC TACAGGATCG GGCACCACAG CACCAGTGAC 
GACAGCTCTG CGTACCGTTC GGTGGATGAG GTCAATTACT GGGACAAGCA GGACCACCCG 
ATCTCGAGAC TGAGGCAGTA CCTGCTGAAC CAGGGTTGGT GGGATGAGGA ACAGGAGAAG 
GCCTGGCGGA AGCAGTCACG AAAGAAGGTG ATGGAGGCCT TTGAACAGGC TGAGCGGAAG 
CTAAAGCCCA ACCCAAGCCT CCTCTTCTCC GATGTGTACC AGGAGATGCC TGCCCAGCTG 
CGCAGGCAGC AGGAGTCCCT GGCTCGGCAC CTGCAGACCT ACGGGGAGCA CTACCCCCTG 
GACCACTTTG ACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444303.1
CDS	39..1196
Translation	MAVAMSAAKIWRPSRGLRQAALLLLGRPGARGLARFLPQEEVLKLYRSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALERTDLVFGQYREAGVLMYRDYPLELFMAQCYGNVSDPGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANANQIVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHPISRLRQYLLNQGWWDEEQEKAWRKQSRKKVMEAFEQAERKLKPNPSLLFSDVYQEMPAQLRRQQESLARHLQTYGEHYPLDHFDK

Target ORF information:

RefSeq Version	XM_017588814.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus branched chain ketoacid dehydrogenase E1, alpha polypeptide (Bckdha), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588814.1

ORF Insert Sequence:

ATGGCGGTAG CGATGTCCGC GGCCAAGATC TGGAGGCCGA GCCGTGGCCT GCGCCAGGCT 
GCCCTCTTGC TGTTGGGGCG ACCTGGGGCT CGGGGCTTGG CTAGATTCCT GCCCCAGGAG 
GAGGTCCTGA AGTTGTACAG GAGCATGACG CTGCTCAACA CCATGGACCG CATTCTCTAT 
GAGTCCCAGC GACAGGGCCG GATCTCCTTC TACATGACCA ACTATGGCGA GGAGGGGACA 
CATGTGGGGA GTGCCGCTGC CCTGGAGCGA ACGGACCTGG TGTTTGGCCA GTACCGGGAG 
GCAGGTGTGC TCATGTACCG GGACTACCCG CTGGAGCTGT TCATGGCCCA GTGCTACGGC 
AACGTGAGTG ACCCAGGCAA GGGACGCCAG ATGCCCGTTC ACTACGGCTG CAAGGAGCGT 
CACTTCGTCA CCATTTCCTC TCCACTGGCT ACGCAGATCC CTCAGGCGGT AGGGGCAGCC 
TATGCTGCCA AGCGGGCCAA TGCCAATCAG ATCGTGATCT GTTACTTTGG CGAGGGGGCA 
GCCAGTGAAG GGGATGCCCA CGCCGGTTTC AATTTTGCTG CCACCTTGGA GTGTCCTATC 
ATCTTCTTCT GCCGGAACAA TGGCTATGCC ATCTCCACAC CAACCTCTGA GCAGTACCGT 
GGGGATGGCA TAGCGGCTCG GGGCCCTGGG TATGGCATCA TGTCAATCCG TGTGGACGGC 
AACGATGTGT TTGCGGTGTA CAATGCCACT AAGGAGGCCC GACGGCGGGC TGTGGCTGAG 
AACCAGCCCT TCCTCATTGA GGCCATGACC TACAGGATCG GGCACCACAG CACCAGTGAC 
GACAGCTCTG CGTACCGTTC GGTGGATGAG GTCAATTACT GGGACAAGCA GGACCACCCG 
ATCTCGAGAC TGAGGCAGTA CCTGCTGAAC CAGGGTTGGT GGGATGAGGA ACAGGAGAAG 
GCCTGGCGGA AGCAGTCACG AAAGAAGGTG ATGGAGGCCT TTGAACAGGC TGAGCGGAAG 
CTAAAGCCCA ACCCAAGCCT CCTCTTCTCC GATGTGTACC AGGAGATGCC TGCCCAGCTG 
CGCAGGCAGC AGGAGTCCCT GGCTCGGCAC CTGCAGACCT ACGGGGAGCA CTACCCCCTG 
GACCACTTTG ACAAGTGA