Chrm4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chrm4
Entrez Gene ID	25111
Full Name	cholinergic receptor, muscarinic 4
Synonyms	M4
General protein information	Preferred Names cholinergic receptor, muscarinic 4 Names muscarinic acetylcholine receptor M4 cholinergic receptor, muscarin 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q24
Summary	a G-protein coupled receptor for acetylcholine; important for mediating the effects of acetylcholine in the nervous system, exocrine glands and smooth muscle [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008762022.2	XP_008760244.1	muscarinic acetylcholine receptor M4 isoform X1
NM_031547.1	NP_113735.1	muscarinic acetylcholine receptor M4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CHRM4	XP_005631350.1
Rattus norvegicus (Norway rat)	Chrm4	NP_113735.1
Gallus gallus (chicken)	CHRM4	NP_001026362.1
Homo sapiens (human)	CHRM4	NP_000732.2
Mus musculus (house mouse)	Chrm4	NP_031725.1
Danio rerio (zebrafish)	chrm4a	XP_001922442.1
Macaca mulatta (Rhesus monkey)	CHRM4	XP_001112370.1
Xenopus tropicalis (tropical clawed frog)	chrm4	NP_001106514.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of basal and amphetamine-stimulated extracellular signal-regulated kinase (ERK) phosphorylation in the rat forebrain by muscarinic acetylcholine M4 receptors.
He N, Mao LM, Sturich AW, Jin DZ, Wang JQ
Brain research1688103-112(2018 06)

Muscarinic signaling in the brain.
Thiele A
Annual review of neuroscience36271-94(2013 Jul)

Muscarinic receptor subtypes as potential targets to modulate oligodendrocyte progenitor survival, proliferation, and differentiation.
De Angelis F, Bernardo A, Magnaghi V, Minghetti L, Tata AM
Developmental neurobiology72(5)713-28(2012 May)

Role of various subtypes of muscarinic cholinergic receptors in the development of posthemorrhagic abnormalities in systemic and portal circulation in rats.
Kovalenko NY, Matsievskii DD, Reshetnyak VK
Bulletin of experimental biology and medicine152(3)293-7(2012 Jan)

Diabetic neuropathy enhances voltage-activated Ca2+ channel activity and its control by M4 muscarinic receptors in primary sensory neurons.
Cao XH, Byun HS, Chen SR, Pan HL
Journal of neurochemistry119(3)594-603(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Chrm4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chrm4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08062	NM_031547.1 Latest version!	Rattus norvegicus cholinergic receptor, muscarinic 4 (Chrm4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa08062	XM_008762022.2 Latest version!	Rattus norvegicus cholinergic receptor, muscarinic 4 (Chrm4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chrm4 ( NM_031547.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113735.1 Rattus norvegicus cholinergic receptor, muscarinic 4 (Chrm4), mRNA.

Price & Availability↑

CloneID	ORa08062
Clone ID Related Accession (Same CDS sequence)	XM_008762022.2 , NM_031547.1
Accession Version	NM_031547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	muscarinic acetylcholine receptor M4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV213670.1, M16409.1 and CR472483.1. On or before Oct 13, 2007 this sequence version replaced XM_345403.3, XM_001078223.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DV213670.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAACT TCACGCCTGT CAATGGCAGC TCAGCCAATC AGTCTGTGCG CCTGGTCACA 
GCAGCCCACA ACCACCTGGA GACAGTGGAG ATGGTGTTCA TTGCTACAGT GACTGGCTCC 
CTGAGCCTGG TGACTGTTGT GGGTAACATC CTGGTGATGC TGTCCATCAA GGTCAACAGG 
CAGTTGCAGA CGGTCAACAA CTACTTCCTC TTCAGCCTGG CGTGTGCGGA TCTCATCATA 
GGGGCATTCT CTATGAACCT CTACACTTTG TATATCATCA AGGGCTACTG GCCCCTGGGT 
GCCGTGGTCT GTGACTTGTG GCTGGCCCTG GACTATGTGG TGAGCAATGC CTCTGTCATG 
AACCTTCTTA TCATCAGCTT CGATCGTTAC TTCTGCGTCA CCAAACCCCT CACCTATCCC 
GCCCGCCGCA CTACTAAGAT GGCAGGTCTC ATGATCGCAG CTGCCTGGGT CTTGTCCTTT 
GTGCTCTGGG CTCCTGCCAT CTTGTTCTGG CAGTTTGTGG TGGGCAAGAG GACGGTGCCT 
GATAACCAGT GCTTCATCCA GTTCTTGTCC AACCCGGCAG TGACCTTTGG CACCGCCATT 
GCTGCCTTCT ACCTGCCTGT GGTCATCATG ACGGTACTGT ATATTCACAT CTCACTGGCC 
AGCCGCAGCC GCGTTCACAA GCATCGACCT GAGGGCCCCA AGGAGAAGAA GGCCAAGACT 
CTGGCTTTCC TCAAGAGCCC TCTGATGAAG CCGAGTATCA AGAAACCTCC ACCAGGGGGC 
GCTTCTCGAG AGGAACTGCG CAATGGGAAG CTGGAAGAGG CTCCTCCGCC AGCCCTGCCC 
CCGCCTCCAC GCCCAGTGCC TGACAAGGAC ACTTCCAATG AGTCCAGCTC AGGCAGTGCC 
ACCCAGAACA CCAAGGAACG GCCACCCACA GAGCTGTCCA CCGCAGAGGC CACCACTCCA 
GCGCTGCCTG CCCCTACCCT GCAGCCACGA ACCCTCAACC CAGCCTCCAA ATGGTCCAAG 
ATTCAAATTG TAACAAAGCA GACAGGCAAT GAATGTGTGA CGGCCATCGA GATTGTACCT 
GCCACTCCAG CTGGTATGCG CCCAGCAGCC AATGTGGCCC GAAAGTTTGC CAGCATTGCT 
CGCAACCAGG TGCGCAAGAA GCGGCAGATG GCGGCCCGGG AGCGCAAAGT GACTCGGACA 
ATCTTTGCCA TTCTGCTGGC CTTCATCCTC ACCTGGACAC CCTACAATGT CATGGTCCTG 
GTGAACACCT TTTGCCAGAG CTGTATCCCC GAGAGGGTGT GGTCCATCGG CTACTGGCTC 
TGCTACGTCA ACAGCACCAT CAACCCCGCC TGCTATGCAC TCTGCAATGC CACTTTCAAA 
AAGACCTTCC GGCACCTTTT GCTGTGCCAG TATCGGAACA TCGGCACAGC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113735.1
CDS	175..1611
Translation	MANFTPVNGSSANQSVRLVTAAHNHLETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTLYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKTLAFLKSPLMKPSIKKPPPGGASREELRNGKLEEAPPPALPPPPRPVPDKDTSNESSSGSATQNTKERPPTELSTAEATTPALPAPTLQPRTLNPASKWSKIQIVTKQTGNECVTAIEIVPATPAGMRPAANVARKFASIARNQVRKKRQMAARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPERVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYRNIGTAR

Target ORF information:

RefSeq Version	NM_031547.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor, muscarinic 4 (Chrm4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031547.1

ORF Insert Sequence:

ATGGCCAACT TCACGCCTGT CAATGGCAGC TCAGCCAATC AGTCTGTGCG CCTGGTCACA 
GCAGCCCACA ACCACCTGGA GACAGTGGAG ATGGTGTTCA TTGCTACAGT GACTGGCTCC 
CTGAGCCTGG TGACTGTTGT GGGTAACATC CTGGTGATGC TGTCCATCAA GGTCAACAGG 
CAGTTGCAGA CGGTCAACAA CTACTTCCTC TTCAGCCTGG CGTGTGCGGA TCTCATCATA 
GGGGCATTCT CTATGAACCT CTACACTTTG TATATCATCA AGGGCTACTG GCCCCTGGGT 
GCCGTGGTCT GTGACTTGTG GCTGGCCCTG GACTATGTGG TGAGCAATGC CTCTGTCATG 
AACCTTCTTA TCATCAGCTT CGATCGTTAC TTCTGCGTCA CCAAACCCCT CACCTATCCC 
GCCCGCCGCA CTACTAAGAT GGCAGGTCTC ATGATCGCAG CTGCCTGGGT CTTGTCCTTT 
GTGCTCTGGG CTCCTGCCAT CTTGTTCTGG CAGTTTGTGG TGGGCAAGAG GACGGTGCCT 
GATAACCAGT GCTTCATCCA GTTCTTGTCC AACCCGGCAG TGACCTTTGG CACCGCCATT 
GCTGCCTTCT ACCTGCCTGT GGTCATCATG ACGGTACTGT ATATTCACAT CTCACTGGCC 
AGCCGCAGCC GCGTTCACAA GCATCGACCT GAGGGCCCCA AGGAGAAGAA GGCCAAGACT 
CTGGCTTTCC TCAAGAGCCC TCTGATGAAG CCGAGTATCA AGAAACCTCC ACCAGGGGGC 
GCTTCTCGAG AGGAACTGCG CAATGGGAAG CTGGAAGAGG CTCCTCCGCC AGCCCTGCCC 
CCGCCTCCAC GCCCAGTGCC TGACAAGGAC ACTTCCAATG AGTCCAGCTC AGGCAGTGCC 
ACCCAGAACA CCAAGGAACG GCCACCCACA GAGCTGTCCA CCGCAGAGGC CACCACTCCA 
GCGCTGCCTG CCCCTACCCT GCAGCCACGA ACCCTCAACC CAGCCTCCAA ATGGTCCAAG 
ATTCAAATTG TAACAAAGCA GACAGGCAAT GAATGTGTGA CGGCCATCGA GATTGTACCT 
GCCACTCCAG CTGGTATGCG CCCAGCAGCC AATGTGGCCC GAAAGTTTGC CAGCATTGCT 
CGCAACCAGG TGCGCAAGAA GCGGCAGATG GCGGCCCGGG AGCGCAAAGT GACTCGGACA 
ATCTTTGCCA TTCTGCTGGC CTTCATCCTC ACCTGGACAC CCTACAATGT CATGGTCCTG 
GTGAACACCT TTTGCCAGAG CTGTATCCCC GAGAGGGTGT GGTCCATCGG CTACTGGCTC 
TGCTACGTCA ACAGCACCAT CAACCCCGCC TGCTATGCAC TCTGCAATGC CACTTTCAAA 
AAGACCTTCC GGCACCTTTT GCTGTGCCAG TATCGGAACA TCGGCACAGC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrm4 ( XM_008762022.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760244.1 Rattus norvegicus cholinergic receptor, muscarinic 4 (Chrm4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa08062
Clone ID Related Accession (Same CDS sequence)	XM_008762022.2 , NM_031547.1
Accession Version	XM_008762022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	muscarinic acetylcholine receptor M4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAACT TCACGCCTGT CAATGGCAGC TCAGCCAATC AGTCTGTGCG CCTGGTCACA 
GCAGCCCACA ACCACCTGGA GACAGTGGAG ATGGTGTTCA TTGCTACAGT GACTGGCTCC 
CTGAGCCTGG TGACTGTTGT GGGTAACATC CTGGTGATGC TGTCCATCAA GGTCAACAGG 
CAGTTGCAGA CGGTCAACAA CTACTTCCTC TTCAGCCTGG CGTGTGCGGA TCTCATCATA 
GGGGCATTCT CTATGAACCT CTACACTTTG TATATCATCA AGGGCTACTG GCCCCTGGGT 
GCCGTGGTCT GTGACTTGTG GCTGGCCCTG GACTATGTGG TGAGCAATGC CTCTGTCATG 
AACCTTCTTA TCATCAGCTT CGATCGTTAC TTCTGCGTCA CCAAACCCCT CACCTATCCC 
GCCCGCCGCA CTACTAAGAT GGCAGGTCTC ATGATCGCAG CTGCCTGGGT CTTGTCCTTT 
GTGCTCTGGG CTCCTGCCAT CTTGTTCTGG CAGTTTGTGG TGGGCAAGAG GACGGTGCCT 
GATAACCAGT GCTTCATCCA GTTCTTGTCC AACCCGGCAG TGACCTTTGG CACCGCCATT 
GCTGCCTTCT ACCTGCCTGT GGTCATCATG ACGGTACTGT ATATTCACAT CTCACTGGCC 
AGCCGCAGCC GCGTTCACAA GCATCGACCT GAGGGCCCCA AGGAGAAGAA GGCCAAGACT 
CTGGCTTTCC TCAAGAGCCC TCTGATGAAG CCGAGTATCA AGAAACCTCC ACCAGGGGGC 
GCTTCTCGAG AGGAACTGCG CAATGGGAAG CTGGAAGAGG CTCCTCCGCC AGCCCTGCCC 
CCGCCTCCAC GCCCAGTGCC TGACAAGGAC ACTTCCAATG AGTCCAGCTC AGGCAGTGCC 
ACCCAGAACA CCAAGGAACG GCCACCCACA GAGCTGTCCA CCGCAGAGGC CACCACTCCA 
GCGCTGCCTG CCCCTACCCT GCAGCCACGA ACCCTCAACC CAGCCTCCAA ATGGTCCAAG 
ATTCAAATTG TAACAAAGCA GACAGGCAAT GAATGTGTGA CGGCCATCGA GATTGTACCT 
GCCACTCCAG CTGGTATGCG CCCAGCAGCC AATGTGGCCC GAAAGTTTGC CAGCATTGCT 
CGCAACCAGG TGCGCAAGAA GCGGCAGATG GCGGCCCGGG AGCGCAAAGT GACTCGGACA 
ATCTTTGCCA TTCTGCTGGC CTTCATCCTC ACCTGGACAC CCTACAATGT CATGGTCCTG 
GTGAACACCT TTTGCCAGAG CTGTATCCCC GAGAGGGTGT GGTCCATCGG CTACTGGCTC 
TGCTACGTCA ACAGCACCAT CAACCCCGCC TGCTATGCAC TCTGCAATGC CACTTTCAAA 
AAGACCTTCC GGCACCTTTT GCTGTGCCAG TATCGGAACA TCGGCACAGC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760244.1
CDS	959..2395
Translation	MANFTPVNGSSANQSVRLVTAAHNHLETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTLYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKTLAFLKSPLMKPSIKKPPPGGASREELRNGKLEEAPPPALPPPPRPVPDKDTSNESSSGSATQNTKERPPTELSTAEATTPALPAPTLQPRTLNPASKWSKIQIVTKQTGNECVTAIEIVPATPAGMRPAANVARKFASIARNQVRKKRQMAARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPERVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYRNIGTAR

Target ORF information:

RefSeq Version	XM_008762022.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor, muscarinic 4 (Chrm4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762022.2

ORF Insert Sequence:

ATGGCCAACT TCACGCCTGT CAATGGCAGC TCAGCCAATC AGTCTGTGCG CCTGGTCACA 
GCAGCCCACA ACCACCTGGA GACAGTGGAG ATGGTGTTCA TTGCTACAGT GACTGGCTCC 
CTGAGCCTGG TGACTGTTGT GGGTAACATC CTGGTGATGC TGTCCATCAA GGTCAACAGG 
CAGTTGCAGA CGGTCAACAA CTACTTCCTC TTCAGCCTGG CGTGTGCGGA TCTCATCATA 
GGGGCATTCT CTATGAACCT CTACACTTTG TATATCATCA AGGGCTACTG GCCCCTGGGT 
GCCGTGGTCT GTGACTTGTG GCTGGCCCTG GACTATGTGG TGAGCAATGC CTCTGTCATG 
AACCTTCTTA TCATCAGCTT CGATCGTTAC TTCTGCGTCA CCAAACCCCT CACCTATCCC 
GCCCGCCGCA CTACTAAGAT GGCAGGTCTC ATGATCGCAG CTGCCTGGGT CTTGTCCTTT 
GTGCTCTGGG CTCCTGCCAT CTTGTTCTGG CAGTTTGTGG TGGGCAAGAG GACGGTGCCT 
GATAACCAGT GCTTCATCCA GTTCTTGTCC AACCCGGCAG TGACCTTTGG CACCGCCATT 
GCTGCCTTCT ACCTGCCTGT GGTCATCATG ACGGTACTGT ATATTCACAT CTCACTGGCC 
AGCCGCAGCC GCGTTCACAA GCATCGACCT GAGGGCCCCA AGGAGAAGAA GGCCAAGACT 
CTGGCTTTCC TCAAGAGCCC TCTGATGAAG CCGAGTATCA AGAAACCTCC ACCAGGGGGC 
GCTTCTCGAG AGGAACTGCG CAATGGGAAG CTGGAAGAGG CTCCTCCGCC AGCCCTGCCC 
CCGCCTCCAC GCCCAGTGCC TGACAAGGAC ACTTCCAATG AGTCCAGCTC AGGCAGTGCC 
ACCCAGAACA CCAAGGAACG GCCACCCACA GAGCTGTCCA CCGCAGAGGC CACCACTCCA 
GCGCTGCCTG CCCCTACCCT GCAGCCACGA ACCCTCAACC CAGCCTCCAA ATGGTCCAAG 
ATTCAAATTG TAACAAAGCA GACAGGCAAT GAATGTGTGA CGGCCATCGA GATTGTACCT 
GCCACTCCAG CTGGTATGCG CCCAGCAGCC AATGTGGCCC GAAAGTTTGC CAGCATTGCT 
CGCAACCAGG TGCGCAAGAA GCGGCAGATG GCGGCCCGGG AGCGCAAAGT GACTCGGACA 
ATCTTTGCCA TTCTGCTGGC CTTCATCCTC ACCTGGACAC CCTACAATGT CATGGTCCTG 
GTGAACACCT TTTGCCAGAG CTGTATCCCC GAGAGGGTGT GGTCCATCGG CTACTGGCTC 
TGCTACGTCA ACAGCACCAT CAACCCCGCC TGCTATGCAC TCTGCAATGC CACTTTCAAA 
AAGACCTTCC GGCACCTTTT GCTGTGCCAG TATCGGAACA TCGGCACAGC CAGGTAG