Plcd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plcd1
Entrez Gene ID	24655
Full Name	phospholipase C, delta 1
Synonyms	Plc1
General protein information	Preferred Names phospholipase C, delta 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 PLC-III PLC-delta-1 Phospholipase C-delta1 phosphoinositide phospholipase C phosphoinositide phospholipase C-delta-1 phospholipase C delta 1 long form phospholipase C-III
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32

mRNA and Protein(s)

mRNA	Protein	Name
NM_017035.1	NP_058731.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1
XM_006244063.3	XP_006244125.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Plcd1	NP_062650.1
Oryza sativa (rice)	Os05g0127200	NP_001054531.1
Homo sapiens (human)	PLCD1	NP_001124436.1
Macaca mulatta (Rhesus monkey)	PLCD1	XP_001089399.1
Pan troglodytes (chimpanzee)	PLCD1	XP_003309769.2
Rattus norvegicus (Norway rat)	Plcd1	NP_058731.1
Canis lupus familiaris (dog)	PLCD1	XP_859460.2
Xenopus tropicalis (tropical clawed frog)	plcd1	XP_004915652.1
Bos taurus (cattle)	PLCD1	NP_001075045.1
Gallus gallus (chicken)	PLCD1	XP_418522.2

Gene Ontology
Bibliography

Related articles in PubMed

Simultaneous loss of phospholipase Cδ1 and phospholipase Cδ3 causes cardiomyocyte apoptosis and cardiomyopathy.
Nakamura Y, Kanemaru K, Kojima R, Hashimoto Y, Marunouchi T, Oka N, Ogura T, Tanonaka K, Fukami K
Cell death & disease5e1215(2014 May)

Ca2+-independent binding of anionic phospholipids by phospholipase C δ1 EF-hand domain.
Cai J, Guo S, Lomasney JW, Roberts MF
The Journal of biological chemistry288(52)37277-88(2013 Dec)

Phospholipase C-δ(1) regulates interleukin-1β and tumor necrosis factor-α mRNA expression.
Chung E, Jakinovich P, Bae A, Rebecchi M
Experimental cell research318(16)1987-93(2012 Oct)

Phospholipase C-delta gene of the spontaneously hypertensive rat harbors point mutations causing amino acid substitutions in a catalytic domain.
Yagisawa H, Tanase H, Nojima H
Journal of hypertension9(11)997-1004(1991 Nov)

Cloning and sequence of multiple forms of phospholipase C.
Suh PG, Ryu SH, Moon KH, Suh HW, Rhee SG
Cell54(2)161-9(1988 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Plcd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plcd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02576	NM_017035.1 Latest version!	Rattus norvegicus phospholipase C, delta 1 (Plcd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa31744	XM_006244063.3 Latest version!	Rattus norvegicus phospholipase C, delta 1 (Plcd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plcd1 ( NM_017035.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058731.1 Rattus norvegicus phospholipase C, delta 1 (Plcd1), mRNA.

Price & Availability↑

CloneID	ORa02576
Clone ID Related Accession (Same CDS sequence)	NM_017035.1
Accession Version	NM_017035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M20637.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M20637.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGGACTCGG GTAGGGACTT CCTGACCCTG CACGGGCTCC AGGATGACCC GGACCTTCAG 
GCCCTTCTGA AGGGCAGCCA GCTTCTGAAG GTGAAGTCCA GCTCGTGGCG TAGGGAACGC 
TTCTACAAGC TACAGGAGGA CTGCAAGACC ATCTGGCAGG AATCTCGAAA GGTCATGAGG 
TCCCCGGAGT CGCAGCTGTT CTCCATCGAG GACATTCAGG AGGTACGGAT GGGACACCGC 
ACAGAAGGCC TGGAGAAGTT TGCCCGAGAC ATCCCCGAGG ATCGATGCTT CTCCATTGTC 
TTCAAGGACC AGCGCAACAC CCTAGACCTC ATTGCCCCAT CACCAGCTGA CGCTCAGCAC 
TGGGTGCAGG GCCTGCGCAA GATCATCCAC CACTCCGGCT CCATGGACCA GCGGCAGAAG 
CTGCAGCACT GGATTCACTC CTGCTTGCGA AAGGCTGATA AAAACAAGGA CAACAAGATG 
AACTTCAAGG AGCTGAAGGA CTTCCTGAAG GAGCTCAACA TCCAGGTGGA TGACGGCTAC 
GCCAGGAAGA TCTTCAGGGA ATGTGACCAC TCCCAGACAG ACTCGCTGGA GGATGAGGAG 
ATTGAGACCT TCTACAAGAT GCTGACCCAG CGCGCAGAGA TCGACCGTGC TTTTGAGGAG 
GCCGCAGGAT CCGCGGAGAC CCTGTCGGTG GAGAGGCTAG TGACGTTTCT GCAGCACCAG 
CAGCGGGAGG AGGAGGCAGG GCCAGCCTTG GCCCTCTCTC TCATTGAGCG CTACGAGCCC 
AGTGAGACTG CCAAGGCCCA GCGGCAGATG ACCAAGGATG GCTTCCTCAT GTACTTGCTG 
TCGGCTGACG GTAACGCCTT CAGCCTGGCA CACCGCCGTG TCTACCAGGA CATGGACCAG 
CCACTGAGTC ACTACTTAGT GTCTTCTTCC CACAACACCT ACCTGCTGGA AGACCAGCTC 
ACAGGGCCCA GCAGCACGGA GGCCTACATC CGGGCTCTGT GCAAAGGCTG CCGGTGCTTG 
GAGCTCGACT GCTGGGATGG TCCCAACCAG GAACCCATCA TCTACCACGG CTACACTTTT 
ACCTCTAAGA TACTTTTCTG TGACGTGCTC AGGGCCATCA GGGACTATGC CTTCAAGGCA 
TCCCCCTACC CAGTCATCCT GTCCCTGGAG AACCACTGTA GCCTGGAGCA ACAACGAGTC 
ATGGCCCGTC ACCTGAGGGC TATCCTGGGG CCCATACTGT TGGACCAGCC ACTGGATGGG 
GTTACCACGA GCCTGCCTTC ACCTGAGCAA CTGAAGGGCA AGATCCTGTT GAAAGGGAAG 
AAGCTGGGAG GGCTGCTGCC TGCTGGAGGG GAGAATGGGT CCGAAGCCAC TGACGTGTCT 
GACGAGGTAG AGGCTGCTGA AATGGAGGAC GAGGCTGTGC GCAGCCAAGT GCAACACAAG 
CCCAAGGAGG ATAAACTAAA GCTGGTGCCG GAGCTCTCCG ACATGATCAT TTACTGCAAG 
AGCGTCCACT TTGGTGGCTT CTCCAGTCCT GGCACCTCTG GGCAGGCATT CTATGAGATG 
GCTTCCTTCT CTGAGAGCCG TGCCCTCCGG TTGCTGCAAG AATCAGGAAA TGGTTTTGTC 
CGTCATAACG TGAGCTGTCT GAGCAGGATC TACCCGGCTG GGTGGAGAAC AGATTCCTCC 
AACTACAGTC CTGTGGAGAT GTGGAACGGG GGCTGCCAGA TTGTTGCTCT GAACTTCCAA 
ACCCCTGGGC CAGAAATGGA TGTTTACCTT GGCTGCTTCC AGGACAATGG AGGCTGTGGG 
TATGTGCTGA AACCCGCCTT TCTGAGAGAC CCCAACACCA CCTTCAACTC ACGTGCCCTG 
ACTCAGGGGC CCTGGTGGCG TCCAGAGAGG CTCCGTGTCC GGATCATCTC TGGACAGCAG 
CTGCCAAAGG TCAATAAGAA TAAGAATTCT ATCGTGGACC CCAAGGTGAT CGTGGAGATC 
CATGGCGTGG GCCGGGACAC CGGTAGCCGT CAGACAGCAG TTATTACCAA TAATGGTTTC 
AACCCACGGT GGGACATGGA GTTTGAGTTT GAGGTAACAG TGCCTGACCT TGCCCTCGTA 
CGCTTCATGG TAGAGGATTA TGATTCCTCT TCTAAGAACG ACTTTATTGG CCAGAGTACT 
ATCCCCTGGA ACAGCCTCAA GCAGGGGTAC CGCCATGTCC ACCTCTTGTC TAAGAATGGA 
GACCAGCATC CATCAGCCAC ACTTTTTGTG AAGATCTCCA TCCAGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058731.1
CDS	223..2493
Translation	MDSGRDFLTLHGLQDDPDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRSPESQLFSIEDIQEVRMGHRTEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPSPADAQHWVQGLRKIIHHSGSMDQRQKLQHWIHSCLRKADKNKDNKMNFKELKDFLKELNIQVDDGYARKIFRECDHSQTDSLEDEEIETFYKMLTQRAEIDRAFEEAAGSAETLSVERLVTFLQHQQREEEAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGNAFSLAHRRVYQDMDQPLSHYLVSSSHNTYLLEDQLTGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRVMARHLRAILGPILLDQPLDGVTTSLPSPEQLKGKILLKGKKLGGLLPAGGENGSEATDVSDEVEAAEMEDEAVRSQVQHKPKEDKLKLVPELSDMIIYCKSVHFGGFSSPGTSGQAFYEMASFSESRALRLLQESGNGFVRHNVSCLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLKPAFLRDPNTTFNSRALTQGPWWRPERLRVRIISGQQLPKVNKNKNSIVDPKVIVEIHGVGRDTGSRQTAVITNNGFNPRWDMEFEFEVTVPDLALVRFMVEDYDSSSKNDFIGQSTIPWNSLKQGYRHVHLLSKNGDQHPSATLFVKISIQD

Target ORF information:

RefSeq Version	NM_017035.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C, delta 1 (Plcd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017035.1

ORF Insert Sequence:

ATGGACTCGG GTAGGGACTT CCTGACCCTG CACGGGCTCC AGGATGACCC GGACCTTCAG 
GCCCTTCTGA AGGGCAGCCA GCTTCTGAAG GTGAAGTCCA GCTCGTGGCG TAGGGAACGC 
TTCTACAAGC TACAGGAGGA CTGCAAGACC ATCTGGCAGG AATCTCGAAA GGTCATGAGG 
TCCCCGGAGT CGCAGCTGTT CTCCATCGAG GACATTCAGG AGGTACGGAT GGGACACCGC 
ACAGAAGGCC TGGAGAAGTT TGCCCGAGAC ATCCCCGAGG ATCGATGCTT CTCCATTGTC 
TTCAAGGACC AGCGCAACAC CCTAGACCTC ATTGCCCCAT CACCAGCTGA CGCTCAGCAC 
TGGGTGCAGG GCCTGCGCAA GATCATCCAC CACTCCGGCT CCATGGACCA GCGGCAGAAG 
CTGCAGCACT GGATTCACTC CTGCTTGCGA AAGGCTGATA AAAACAAGGA CAACAAGATG 
AACTTCAAGG AGCTGAAGGA CTTCCTGAAG GAGCTCAACA TCCAGGTGGA TGACGGCTAC 
GCCAGGAAGA TCTTCAGGGA ATGTGACCAC TCCCAGACAG ACTCGCTGGA GGATGAGGAG 
ATTGAGACCT TCTACAAGAT GCTGACCCAG CGCGCAGAGA TCGACCGTGC TTTTGAGGAG 
GCCGCAGGAT CCGCGGAGAC CCTGTCGGTG GAGAGGCTAG TGACGTTTCT GCAGCACCAG 
CAGCGGGAGG AGGAGGCAGG GCCAGCCTTG GCCCTCTCTC TCATTGAGCG CTACGAGCCC 
AGTGAGACTG CCAAGGCCCA GCGGCAGATG ACCAAGGATG GCTTCCTCAT GTACTTGCTG 
TCGGCTGACG GTAACGCCTT CAGCCTGGCA CACCGCCGTG TCTACCAGGA CATGGACCAG 
CCACTGAGTC ACTACTTAGT GTCTTCTTCC CACAACACCT ACCTGCTGGA AGACCAGCTC 
ACAGGGCCCA GCAGCACGGA GGCCTACATC CGGGCTCTGT GCAAAGGCTG CCGGTGCTTG 
GAGCTCGACT GCTGGGATGG TCCCAACCAG GAACCCATCA TCTACCACGG CTACACTTTT 
ACCTCTAAGA TACTTTTCTG TGACGTGCTC AGGGCCATCA GGGACTATGC CTTCAAGGCA 
TCCCCCTACC CAGTCATCCT GTCCCTGGAG AACCACTGTA GCCTGGAGCA ACAACGAGTC 
ATGGCCCGTC ACCTGAGGGC TATCCTGGGG CCCATACTGT TGGACCAGCC ACTGGATGGG 
GTTACCACGA GCCTGCCTTC ACCTGAGCAA CTGAAGGGCA AGATCCTGTT GAAAGGGAAG 
AAGCTGGGAG GGCTGCTGCC TGCTGGAGGG GAGAATGGGT CCGAAGCCAC TGACGTGTCT 
GACGAGGTAG AGGCTGCTGA AATGGAGGAC GAGGCTGTGC GCAGCCAAGT GCAACACAAG 
CCCAAGGAGG ATAAACTAAA GCTGGTGCCG GAGCTCTCCG ACATGATCAT TTACTGCAAG 
AGCGTCCACT TTGGTGGCTT CTCCAGTCCT GGCACCTCTG GGCAGGCATT CTATGAGATG 
GCTTCCTTCT CTGAGAGCCG TGCCCTCCGG TTGCTGCAAG AATCAGGAAA TGGTTTTGTC 
CGTCATAACG TGAGCTGTCT GAGCAGGATC TACCCGGCTG GGTGGAGAAC AGATTCCTCC 
AACTACAGTC CTGTGGAGAT GTGGAACGGG GGCTGCCAGA TTGTTGCTCT GAACTTCCAA 
ACCCCTGGGC CAGAAATGGA TGTTTACCTT GGCTGCTTCC AGGACAATGG AGGCTGTGGG 
TATGTGCTGA AACCCGCCTT TCTGAGAGAC CCCAACACCA CCTTCAACTC ACGTGCCCTG 
ACTCAGGGGC CCTGGTGGCG TCCAGAGAGG CTCCGTGTCC GGATCATCTC TGGACAGCAG 
CTGCCAAAGG TCAATAAGAA TAAGAATTCT ATCGTGGACC CCAAGGTGAT CGTGGAGATC 
CATGGCGTGG GCCGGGACAC CGGTAGCCGT CAGACAGCAG TTATTACCAA TAATGGTTTC 
AACCCACGGT GGGACATGGA GTTTGAGTTT GAGGTAACAG TGCCTGACCT TGCCCTCGTA 
CGCTTCATGG TAGAGGATTA TGATTCCTCT TCTAAGAACG ACTTTATTGG CCAGAGTACT 
ATCCCCTGGA ACAGCCTCAA GCAGGGGTAC CGCCATGTCC ACCTCTTGTC TAAGAATGGA 
GACCAGCATC CATCAGCCAC ACTTTTTGTG AAGATCTCCA TCCAGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcd1 ( XM_006244063.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006244125.1 Rattus norvegicus phospholipase C, delta 1 (Plcd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa31744
Clone ID Related Accession (Same CDS sequence)	XM_006244063.3
Accession Version	XM_006244063.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244063.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCGG GTAGGGACTT CCTGACCCTG CACGGGCTCC AGGATGACCC GGACCTTCAG 
GCCCTTCTGA AGGGCAGCCA GCTTCTGAAG GTGAAGTCCA GCTCGTGGCG TAGGGAACGC 
TTCTACAAGC TACAGGAGGA CTGCAAGACC ATCTGGCAGG AATCTCGAAA GGTCATGAGG 
TCCCCGGAGT CGCAGCTGTT CTCCATCGAG GACATTCAGG AGGTACGGAT GGGACACCGC 
ACAGAAGGCC TGGAGAAGTT TGCCCGAGAC ATCCCCGAGG ATCGATGCTT CTCCATTGTC 
TTCAAGGACC AGCGCAACAC CCTAGACCTC ATTGCCCCAT CACCAGCTGA CGCTCAGCAC 
TGGGTGCAGG GCCTGCGCAA GATCATCCAC CACTCCGGCT CCATGGACCA GCGGCAGAAG 
CTGCAGCACT GGATTCACTC CTGCTTGCGA AAGGCTGATA AAAACAAGGA CAACAAGATG 
AACTTCAAGG AGCTGAAGGA CTTCCTGAAG GAGCTCAACA TCCAGGTGGA TGACGGCTAC 
GCCAGGAAGA TCTTCAGGGA ATGTGACCAC TCCCAGACAG ACTCGCTGGA GGATGAGGAG 
ATTGAGACCT TCTACAAGAT GCTGACCCAG CGCGCAGAGA TCGACCGTGC TTTTGAGGAG 
GCCGCAGGAT CCGCGGAGAC CCTGTCGGTG GAGAGGCTAG TGACGTTTCT GCAGCACCAG 
CAGCGGGAGG AGGAGGCAGG GCCAGCCTTG GCCCTCTCTC TCATTGAGCG CTACGAGCCC 
AGTGAGACTG CCAAGGCCCA GCGGCAGATG ACCAAGGATG GCTTCCTCAT GTACTTGCTG 
TCGGCTGACG GTAACGCCTT CAGCCTGGCA CACCGCCGTG TCTACCAGGA CATGGACCAG 
CCACTGAGTC ACTACTTAGT GTCTTCTTCC CACAACACCT ACCTGCTGGA AGACCAGCTC 
ACAGGGCCCA GCAGCACGGA GGCCTACATC CGGGCTCTGT GCAAAGGCTG CCGGTGCTTG 
GAGCTCGACT GCTGGGATGG TCCCAACCAG GAACCCATCA TCTACCACGG CTACACTTTT 
ACCTCTAAGA TACTTTTCTG TGACGTGCTC AGGGCCATCA GGGACTATGC CTTCAAGGCA 
TCCCCCTACC CAGTCATCCT GTCCCTGGAG AACCACTGTA GCCTGGAGCA ACAACGAGTC 
ATGGCCCGTC ACCTGAGGGC TATCCTGGGG CCCATGCTGT TGGACCAGCC ACTGGATGGG 
GTTACCTCGA GCCTGCCTTC ACCTGAGCAA CTGAAGGGCA AGATCCTGTT GAAAGGGAAG 
AAGCTGGGAG GGCTGCTGCC TGCTGGAGGG GAGAATGGGT CCGAAGCCAC TGACGTGTCT 
GACGAGGATG AGGCTGCTGA AATGGAGGAC GAGGCTGTGC GCAGCCAAGT GCAACACAAG 
CCCAAGGAGG ATAAACTAAA GCTGGTGCCG GAGCTCTCCG ACATGATCAT TTACTGCAAG 
AGCGTCCACT TTGGTGGCTT CTCCAGTCCT GGCACCTCTG GGCAGGCATT CTATGAGATG 
GCTTCCTTTT CTGAGAGCCG TGCCCTCCGG TTGCTGCAAG AATCAGGAAA TGGTTTTGTC 
CGTCATAACG TGAGCTGTCT GAGCAGGATC TACCCGGCTG GGTGGAGAAC AGATTCCTCC 
AACTACAGTC CTGTGGAGAT GTGGAACGGG GGCTGCCAGA TTGTGGCTCT GAACTTCCAA 
ACCCCTGGGC CAGAAATGGA TGTTTACCTT GGCTGCTTCC AGGACAATGG AGGCTGTGGG 
TATGTGCTGA AACCCGCCTT TCTGAGAGAC CCCAACACCA CCTTCAACTC ACGTGCCCTG 
ACTCAGGGGC CCTGGTGGGC TCCGAAGAGG CTCCGTGTCC GGATCATCTC TGGACAGCAG 
CTGCCAAAGG TCAATAAGAA TAAGAATTCT ATCGTGGACC CCAAGGTGAT CGTGGAGATC 
CATGGCGTGG GCCGGGACAC GGCTAGCCGT CAGACAGCAG TTATTACCAA TAATGGTATA 
GTATGCGTTG GGTCGGCGTT GGGCCAGGCT GCTGGTACCG TTGGCCCCGC TGTTGATATG 
GTTTCTGACC TTCTAGGTTT CAACCCACGG TGGGACATGG AGTTTGAGTT TGAGGTAACA 
GTGCCTGACC TTGCCCTCGT ACGCTTCATG GTAGAGGATT ATGATTCCTC TTCTAAGAAC 
GACTTTATTG GCCAGAGTAC TATCCCCTGG AACAGCCTCA AGCAGGGGTA CCGCCATGTC 
CACCTCTTGT CTAAGAATGG AGACCAGCAT CCATCAGCCA CACTTTTTGT GAAGATCTCC 
ATCCAGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006244125.1
CDS	120..2471
Translation	MDSGRDFLTLHGLQDDPDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRSPESQLFSIEDIQEVRMGHRTEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPSPADAQHWVQGLRKIIHHSGSMDQRQKLQHWIHSCLRKADKNKDNKMNFKELKDFLKELNIQVDDGYARKIFRECDHSQTDSLEDEEIETFYKMLTQRAEIDRAFEEAAGSAETLSVERLVTFLQHQQREEEAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGNAFSLAHRRVYQDMDQPLSHYLVSSSHNTYLLEDQLTGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRVMARHLRAILGPMLLDQPLDGVTSSLPSPEQLKGKILLKGKKLGGLLPAGGENGSEATDVSDEDEAAEMEDEAVRSQVQHKPKEDKLKLVPELSDMIIYCKSVHFGGFSSPGTSGQAFYEMASFSESRALRLLQESGNGFVRHNVSCLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLKPAFLRDPNTTFNSRALTQGPWWAPKRLRVRIISGQQLPKVNKNKNSIVDPKVIVEIHGVGRDTASRQTAVITNNGIVCVGSALGQAAGTVGPAVDMVSDLLGFNPRWDMEFEFEVTVPDLALVRFMVEDYDSSSKNDFIGQSTIPWNSLKQGYRHVHLLSKNGDQHPSATLFVKISIQD

Target ORF information:

RefSeq Version	XM_006244063.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C, delta 1 (Plcd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244063.3

ORF Insert Sequence:

ATGGACTCGG GTAGGGACTT CCTGACCCTG CACGGGCTCC AGGATGACCC GGACCTTCAG 
GCCCTTCTGA AGGGCAGCCA GCTTCTGAAG GTGAAGTCCA GCTCGTGGCG TAGGGAACGC 
TTCTACAAGC TACAGGAGGA CTGCAAGACC ATCTGGCAGG AATCTCGAAA GGTCATGAGG 
TCCCCGGAGT CGCAGCTGTT CTCCATCGAG GACATTCAGG AGGTACGGAT GGGACACCGC 
ACAGAAGGCC TGGAGAAGTT TGCCCGAGAC ATCCCCGAGG ATCGATGCTT CTCCATTGTC 
TTCAAGGACC AGCGCAACAC CCTAGACCTC ATTGCCCCAT CACCAGCTGA CGCTCAGCAC 
TGGGTGCAGG GCCTGCGCAA GATCATCCAC CACTCCGGCT CCATGGACCA GCGGCAGAAG 
CTGCAGCACT GGATTCACTC CTGCTTGCGA AAGGCTGATA AAAACAAGGA CAACAAGATG 
AACTTCAAGG AGCTGAAGGA CTTCCTGAAG GAGCTCAACA TCCAGGTGGA TGACGGCTAC 
GCCAGGAAGA TCTTCAGGGA ATGTGACCAC TCCCAGACAG ACTCGCTGGA GGATGAGGAG 
ATTGAGACCT TCTACAAGAT GCTGACCCAG CGCGCAGAGA TCGACCGTGC TTTTGAGGAG 
GCCGCAGGAT CCGCGGAGAC CCTGTCGGTG GAGAGGCTAG TGACGTTTCT GCAGCACCAG 
CAGCGGGAGG AGGAGGCAGG GCCAGCCTTG GCCCTCTCTC TCATTGAGCG CTACGAGCCC 
AGTGAGACTG CCAAGGCCCA GCGGCAGATG ACCAAGGATG GCTTCCTCAT GTACTTGCTG 
TCGGCTGACG GTAACGCCTT CAGCCTGGCA CACCGCCGTG TCTACCAGGA CATGGACCAG 
CCACTGAGTC ACTACTTAGT GTCTTCTTCC CACAACACCT ACCTGCTGGA AGACCAGCTC 
ACAGGGCCCA GCAGCACGGA GGCCTACATC CGGGCTCTGT GCAAAGGCTG CCGGTGCTTG 
GAGCTCGACT GCTGGGATGG TCCCAACCAG GAACCCATCA TCTACCACGG CTACACTTTT 
ACCTCTAAGA TACTTTTCTG TGACGTGCTC AGGGCCATCA GGGACTATGC CTTCAAGGCA 
TCCCCCTACC CAGTCATCCT GTCCCTGGAG AACCACTGTA GCCTGGAGCA ACAACGAGTC 
ATGGCCCGTC ACCTGAGGGC TATCCTGGGG CCCATGCTGT TGGACCAGCC ACTGGATGGG 
GTTACCTCGA GCCTGCCTTC ACCTGAGCAA CTGAAGGGCA AGATCCTGTT GAAAGGGAAG 
AAGCTGGGAG GGCTGCTGCC TGCTGGAGGG GAGAATGGGT CCGAAGCCAC TGACGTGTCT 
GACGAGGATG AGGCTGCTGA AATGGAGGAC GAGGCTGTGC GCAGCCAAGT GCAACACAAG 
CCCAAGGAGG ATAAACTAAA GCTGGTGCCG GAGCTCTCCG ACATGATCAT TTACTGCAAG 
AGCGTCCACT TTGGTGGCTT CTCCAGTCCT GGCACCTCTG GGCAGGCATT CTATGAGATG 
GCTTCCTTTT CTGAGAGCCG TGCCCTCCGG TTGCTGCAAG AATCAGGAAA TGGTTTTGTC 
CGTCATAACG TGAGCTGTCT GAGCAGGATC TACCCGGCTG GGTGGAGAAC AGATTCCTCC 
AACTACAGTC CTGTGGAGAT GTGGAACGGG GGCTGCCAGA TTGTGGCTCT GAACTTCCAA 
ACCCCTGGGC CAGAAATGGA TGTTTACCTT GGCTGCTTCC AGGACAATGG AGGCTGTGGG 
TATGTGCTGA AACCCGCCTT TCTGAGAGAC CCCAACACCA CCTTCAACTC ACGTGCCCTG 
ACTCAGGGGC CCTGGTGGGC TCCGAAGAGG CTCCGTGTCC GGATCATCTC TGGACAGCAG 
CTGCCAAAGG TCAATAAGAA TAAGAATTCT ATCGTGGACC CCAAGGTGAT CGTGGAGATC 
CATGGCGTGG GCCGGGACAC GGCTAGCCGT CAGACAGCAG TTATTACCAA TAATGGTATA 
GTATGCGTTG GGTCGGCGTT GGGCCAGGCT GCTGGTACCG TTGGCCCCGC TGTTGATATG 
GTTTCTGACC TTCTAGGTTT CAACCCACGG TGGGACATGG AGTTTGAGTT TGAGGTAACA 
GTGCCTGACC TTGCCCTCGT ACGCTTCATG GTAGAGGATT ATGATTCCTC TTCTAAGAAC 
GACTTTATTG GCCAGAGTAC TATCCCCTGG AACAGCCTCA AGCAGGGGTA CCGCCATGTC 
CACCTCTTGT CTAAGAATGG AGACCAGCAT CCATCAGCCA CACTTTTTGT GAAGATCTCC 
ATCCAGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Simultaneous loss of phospholipase Cδ1 and phospholipase Cδ3 causes cardiomyocyte apoptosis and cardiomyopathy.
Cell death & disease5e1215(2014 May)
Nakamura Y,Kanemaru K,Kojima R,Hashimoto Y,Marunouchi T,Oka N,Ogura T,Tanonaka K,Fukami K

Ca2+-independent binding of anionic phospholipids by phospholipase C δ1 EF-hand domain.
The Journal of biological chemistry288(52)37277-88(2013 Dec)
Cai J,Guo S,Lomasney JW,Roberts MF

Phospholipase C-δ(1) regulates interleukin-1β and tumor necrosis factor-α mRNA expression.
Experimental cell research318(16)1987-93(2012 Oct)
Chung E,Jakinovich P,Bae A,Rebecchi M

Phospholipase C-delta gene of the spontaneously hypertensive rat harbors point mutations causing amino acid substitutions in a catalytic domain.
Journal of hypertension9(11)997-1004(1991 Nov)
Yagisawa H,Tanase H,Nojima H

Cloning and sequence of multiple forms of phospholipase C.
Cell54(2)161-9(1988 Jul)
Suh PG,Ryu SH,Moon KH,Suh HW,Rhee SG

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Plcd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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