Tpcn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tpcn1
Entrez Gene ID	246215
Full Name	two pore segment channel 1
General protein information	Preferred Names two pore segment channel 1 Names two pore calcium channel protein 1 two pore channel 1 voltage-dependent calcium channel protein TPC1 voltage-gated Ca channel
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16
Summary	two-pore voltage-gated sodium and calcium channel [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_139332.3	NP_647548.2	two pore calcium channel protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TPCN1	XP_003313998.1
Canis lupus familiaris (dog)	TPCN1	XP_534690.2
Bos taurus (cattle)	TPCN1	XP_002694563.1
Gallus gallus (chicken)	TPCN1	XP_004934547.1
Homo sapiens (human)	TPCN1	NP_001137291.1
Rattus norvegicus (Norway rat)	Tpcn1	NP_647548.2
Danio rerio (zebrafish)	tpcn1	XP_005165277.1
Xenopus tropicalis (tropical clawed frog)	tpcn1	XP_002934333.2
Macaca mulatta (Rhesus monkey)	TPCN1	XP_002798827.1
Mus musculus (house mouse)	Tpcn1	NP_665852.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural insights into the voltage and phospholipid activation of the mammalian TPC1 channel.
She J, Guo J, Chen Q, Zeng W, Jiang Y, Bai XC
Nature556(7699)130-134(2018 04)

Leucine-rich repeat kinase 2 regulates autophagy through a calcium-dependent pathway involving NAADP.
Gómez-Suaga P, Luzón-Toro B, Churamani D, Zhang L, Bloor-Young D, Patel S, Woodman PG, Churchill GC, Hilfiker S
Human molecular genetics21(3)511-25(2012 Feb)

Two-pore channels form homo- and heterodimers.
Rietdorf K, Funnell TM, Ruas M, Heinemann J, Parrington J, Galione A
The Journal of biological chemistry286(43)37058-62(2011 Oct)

Calcium signaling via two-pore channels: local or global, that is the question.
Zhu MX, Ma J, Parrington J, Calcraft PJ, Galione A, Evans AM
American journal of physiology. Cell physiology298(3)C430-41(2010 Mar)

Essential requirement for two-pore channel 1 in NAADP-mediated calcium signaling.
Brailoiu E, Churamani D, Cai X, Schrlau MG, Brailoiu GC, Gao X, Hooper R, Boulware MJ, Dun NJ, Marchant JS, Patel S
The Journal of cell biology186(2)201-9(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tpcn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tpcn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa39878	NM_139332.3 Latest version!	Rattus norvegicus two pore segment channel 1 (Tpcn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tpcn1 ( NM_139332.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_647548.2 Rattus norvegicus two pore segment channel 1 (Tpcn1), mRNA.

Price & Availability↑

CloneID	ORa39878
Clone ID Related Accession (Same CDS sequence)	NM_139332.3
Accession Version	NM_139332.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570550400000
Organism	Rattus norvegicus(Norway rat)
Product	two pore calcium channel protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC062072.1, AB018253.2, CK473123.1, CA339864.1 and AABR07036390.1. On Sep 15, 2005 this sequence version replaced NM_139332.2. ##Evidence-Data-START## Transcript exon combination :: BC062072.1, AB018253.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGTAA TTTTAGACGA CGACGTGCTG CTCATCCTGA CCTTGGACGA GGAGCTGAGT 
GCGCCGCTGA CTCCTTCCAA CGGCCTGGGC CAAGAAGACC TGCCCAGCAA AAATGGGGGC 
GGCCAAAGCG GCCCCAACTC CCAAGTTCCC AGTCTGGTCT CTGGAGCCGA CAGTCCCCCC 
TCCAGTCCCC CCGGACACAA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG 
GAAGGCCAGA ACAATGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCTGCC 
TACCTCTTCG TACACAACCA CTTCTTTTAT ATGATGGAGC TGCTCACAGC CCTGCTTCTG 
CTGCTGCTGT CACTGTGCGA GTCACCCGCT GTCCCTGCGC TCAAGCTCCG AACTTACGTC 
CACGCCACCC TGGAGCTGTT CGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCCACAC ATTCGTCAGG CACAAACGCA CCATGGTCAA GACGTCTGTT 
TTGGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTGC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG CATTTTCCTG GTGGACTGTC GGTACTGTGG TGGCGTGCGG 
CGCAACCTGC GGCAGATCTT CCAGTCGCTG CCGCCTTTCA TGGACATCCT CCTGCTGCTG 
CTCTTCTTTA TGATCATCTT CGCCATCCTC GGTTTCTACT TATTCTCTAC GAACCCTTCC 
GACCCTTACT TCAACACCCT GGAGAACAGC ATCGTCAACC TGTTTGTTCT CCTGACCACA 
GCCAACTTTC CAGACGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGTGTCTTC 
TTTATCGTGT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT AGCCGTGGTG 
TTCGACACCT TCAACGACAT CGAAAAGCAC AAGTTCAAGT CCTTGCTGCT GCATAAGCGA 
ACAGCCATCC AGCACGCATA CCACCTGCTC GTCAGCCAAC GGAGGCCGGC CGGCATCTCC 
TACAGGCAGT TTGAAGGCTT GATGCGCTTC TACAAGCCCC GGATGAGCGC TAGGGAGCGC 
TTCCTGACTT TCAAGGCCCT GAATCAGAGC AACACACCTC TGCTTAGCCT GAAGGACTTC 
TATGATATTT ATGAAGTTGC TGCTCTGCAG TGGAAGGCAA AGAAGAACAG ACAGCACTGG 
TTTGACGAGC TTCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACCTG GTGGTGGCTG TCAACGGTGT CTGGATCCTA 
GTGGAGACAT TCATGTTGAA AGGTGGGAAT TTCATCTCAA AGCACGTGCC CTGGAGTTAC 
CTCGTGTTTC TTACCATCTA TGGAGTTGAG CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACT TGTCCTCTGG ATGGAACCTG TTCGATTTCT CAGTCACAGC ATTTGCCTTC 
CTGGGACTGC TCGCGCTGAC GCTCAACATG GAGCCCTTCT ACTTTATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTCAAGTTG AAGAAACGCT ACCGCAATGT GTTGGACACC 
ATGTTTGAGC TGCTGCCACG AATGGCCAGC CTCGGCCTCA CACTGCTCAC CTTCTACTAC 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AGTGGGCGAC TGTCCCCCAA CTGCTGCAAC 
TCCAGCACGG TGGCTGACGC CTATCGATTC ATCAATCACA CGGTGGGCAA TAAGACCAAG 
GTGGAGGAAG GCTACTACTA TCTCAATAAC TTTGACAACA TCCTCAACAG CTTTGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAACTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACTTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TCGTGACCAT GGTGGTGATG 
ACCATTATCG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGAAT CGGAAGTGGA CAGTGGCATC GTCATCGAGA AGGAGATGTC CAAGGAGGAG 
CTTCTGGCCA TCCTGGAGCT TCATCGAGAG GCGCGAGGCA CCTCCTCTGA CATCACCCGG 
CTGCTCGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ACTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGC CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGCAGC TCAGGGGCAG CGCGCCAAGC 
CCTGCAGCCC AGCAGACCCC CGGCAGTCGC CAGCGCTCCC AGACCGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647548.2
CDS	254..2707
Translation	MSVILDDDVLLILTLDEELSAPLTPSNGLGQEDLPSKNGGGQSGPNSQVPSLVSGADSPPSSPPGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPALKLRTYVHATLELFALMVVVFELCMKLRWLGFHTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFNTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYHLLVSQRRPAGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKKNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFISKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFSGRLSPNCCNSSTVADAYRFINHTVGNKTKVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQESEVDSGIVIEKEMSKEELLAILELHREARGTSSDITRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQQLRGSAPSPAAQQTPGSRQRSQTVT

Target ORF information:

RefSeq Version	NM_139332.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus two pore segment channel 1 (Tpcn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139332.3

ORF Insert Sequence:

ATGTCTGTAA TTTTAGACGA CGACGTGCTG CTCATCCTGA CCTTGGACGA GGAGCTGAGT 
GCGCCGCTGA CTCCTTCCAA CGGCCTGGGC CAAGAAGACC TGCCCAGCAA AAATGGGGGC 
GGCCAAAGCG GCCCCAACTC CCAAGTTCCC AGTCTGGTCT CTGGAGCCGA CAGTCCCCCC 
TCCAGTCCCC CCGGACACAA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG 
GAAGGCCAGA ACAATGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCTGCC 
TACCTCTTCG TACACAACCA CTTCTTTTAT ATGATGGAGC TGCTCACAGC CCTGCTTCTG 
CTGCTGCTGT CACTGTGCGA GTCACCCGCT GTCCCTGCGC TCAAGCTCCG AACTTACGTC 
CACGCCACCC TGGAGCTGTT CGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCCACAC ATTCGTCAGG CACAAACGCA CCATGGTCAA GACGTCTGTT 
TTGGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTGC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG CATTTTCCTG GTGGACTGTC GGTACTGTGG TGGCGTGCGG 
CGCAACCTGC GGCAGATCTT CCAGTCGCTG CCGCCTTTCA TGGACATCCT CCTGCTGCTG 
CTCTTCTTTA TGATCATCTT CGCCATCCTC GGTTTCTACT TATTCTCTAC GAACCCTTCC 
GACCCTTACT TCAACACCCT GGAGAACAGC ATCGTCAACC TGTTTGTTCT CCTGACCACA 
GCCAACTTTC CAGACGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGTGTCTTC 
TTTATCGTGT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT AGCCGTGGTG 
TTCGACACCT TCAACGACAT CGAAAAGCAC AAGTTCAAGT CCTTGCTGCT GCATAAGCGA 
ACAGCCATCC AGCACGCATA CCACCTGCTC GTCAGCCAAC GGAGGCCGGC CGGCATCTCC 
TACAGGCAGT TTGAAGGCTT GATGCGCTTC TACAAGCCCC GGATGAGCGC TAGGGAGCGC 
TTCCTGACTT TCAAGGCCCT GAATCAGAGC AACACACCTC TGCTTAGCCT GAAGGACTTC 
TATGATATTT ATGAAGTTGC TGCTCTGCAG TGGAAGGCAA AGAAGAACAG ACAGCACTGG 
TTTGACGAGC TTCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACCTG GTGGTGGCTG TCAACGGTGT CTGGATCCTA 
GTGGAGACAT TCATGTTGAA AGGTGGGAAT TTCATCTCAA AGCACGTGCC CTGGAGTTAC 
CTCGTGTTTC TTACCATCTA TGGAGTTGAG CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACT TGTCCTCTGG ATGGAACCTG TTCGATTTCT CAGTCACAGC ATTTGCCTTC 
CTGGGACTGC TCGCGCTGAC GCTCAACATG GAGCCCTTCT ACTTTATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTCAAGTTG AAGAAACGCT ACCGCAATGT GTTGGACACC 
ATGTTTGAGC TGCTGCCACG AATGGCCAGC CTCGGCCTCA CACTGCTCAC CTTCTACTAC 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AGTGGGCGAC TGTCCCCCAA CTGCTGCAAC 
TCCAGCACGG TGGCTGACGC CTATCGATTC ATCAATCACA CGGTGGGCAA TAAGACCAAG 
GTGGAGGAAG GCTACTACTA TCTCAATAAC TTTGACAACA TCCTCAACAG CTTTGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAACTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACTTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TCGTGACCAT GGTGGTGATG 
ACCATTATCG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGAAT CGGAAGTGGA CAGTGGCATC GTCATCGAGA AGGAGATGTC CAAGGAGGAG 
CTTCTGGCCA TCCTGGAGCT TCATCGAGAG GCGCGAGGCA CCTCCTCTGA CATCACCCGG 
CTGCTCGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ACTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGC CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGCAGC TCAGGGGCAG CGCGCCAAGC 
CCTGCAGCCC AGCAGACCCC CGGCAGTCGC CAGCGCTCCC AGACCGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural insights into the voltage and phospholipid activation of the mammalian TPC1 channel.
Nature556(7699)130-134(2018 04)
She J,Guo J,Chen Q,Zeng W,Jiang Y,Bai XC

Leucine-rich repeat kinase 2 regulates autophagy through a calcium-dependent pathway involving NAADP.
Human molecular genetics21(3)511-25(2012 Feb)
Gómez-Suaga P,Luzón-Toro B,Churamani D,Zhang L,Bloor-Young D,Patel S,Woodman PG,Churchill GC,Hilfiker S

Two-pore channels form homo- and heterodimers.
The Journal of biological chemistry286(43)37058-62(2011 Oct)
Rietdorf K,Funnell TM,Ruas M,Heinemann J,Parrington J,Galione A

Calcium signaling via two-pore channels: local or global, that is the question.
American journal of physiology. Cell physiology298(3)C430-41(2010 Mar)
Zhu MX,Ma J,Parrington J,Calcraft PJ,Galione A,Evans AM

Essential requirement for two-pore channel 1 in NAADP-mediated calcium signaling.
The Journal of cell biology186(2)201-9(2009 Jul)
Brailoiu E,Churamani D,Cai X,Schrlau MG,Brailoiu GC,Gao X,Hooper R,Boulware MJ,Dun NJ,Marchant JS,Patel S

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Tpcn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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