Hsd3b7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsd3b7
Entrez Gene ID	246211
Full Name	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
Synonyms	Cca2
General protein information	Preferred Names hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 Names 3 beta-hydroxysteroid dehydrogenase type 7 3 beta-hydroxysteroid dehydrogenase type VII 3-beta-HSD VII 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase c(27) 3-beta-HSD cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase confluent 3Y1 cell-associated 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q37
Summary	protein that is expressed in under growth-arrest conditions [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_139329.1	NP_647545.1	3 beta-hydroxysteroid dehydrogenase type 7
XM_006230212.3	XP_006230274.1	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HSD3B7	NP_079469.2
Rattus norvegicus (Norway rat)	Hsd3b7	NP_647545.1
Xenopus tropicalis (tropical clawed frog)	hsd3b7	NP_001120501.1
Bos taurus (cattle)	HSD3B7	NP_001029868.1
Danio rerio (zebrafish)	hsd3b7	NP_956103.1
Pan troglodytes (chimpanzee)	HSD3B7	XP_001155219.1
Canis lupus familiaris (dog)	HSD3B7	XP_547037.1
Mus musculus (house mouse)	Hsd3b7	NP_598704.2

Gene Ontology
Bibliography

Related articles in PubMed

Oxysterol gradient generation by lymphoid stromal cells guides activated B cell movement during humoral responses.
Yi T, Wang X, Kelly LM, An J, Xu Y, Sailer AW, Gustafsson JA, Russell DW, Cyster JG
Immunity37(3)535-48(2012 Sep)

Bile acid biosynthesis during liver regeneration: enzyme activities of cholesterol 7alpha-hydroxylase and 3beta-hydroxy-delta5-C27-steroid dehydrogenase in rats.
Maeda Y, Nagatomo H, Kuroki N, Nagatomo J, Uchiyama F, Shimozono K, Kohno Y, Chijiiwa K
Annals of clinical and laboratory science35(3)323-8(2005)

Molecular genetics of 3beta-hydroxy-Delta5-C27-steroid oxidoreductase deficiency in 16 patients with loss of bile acid synthesis and liver disease.
Cheng JB, Jacquemin E, Gerhardt M, Nazer H, Cresteil D, Heubi JE, Setchell KD, Russell DW
The Journal of clinical endocrinology and metabolism88(4)1833-41(2003 Apr)

The bile acid synthetic gene 3beta-hydroxy-Delta(5)-C(27)-steroid oxidoreductase is mutated in progressive intrahepatic cholestasis.
Schwarz M, Wright AC, Davis DL, Nazer H, Björkhem I, Russell DW
The Journal of clinical investigation106(9)1175-84(2000 Nov)

Isolation of a novel cDNA whose corresponding mRNA is accumulated in growth-arrested confluent but not in growing sub-confluent rat 3Y1 cells.
Hayashi Y, Kiyono T, Fujita M, Ishibashi M
Biochimica et biophysica acta1352(2)145-50(1997 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hsd3b7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsd3b7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02360	NM_139329.1 Latest version!	Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa23402	XM_006230212.3 Latest version!	Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsd3b7 ( NM_139329.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_647545.1 Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), mRNA.

Price & Availability↑

CloneID	ORa02360
Clone ID Related Accession (Same CDS sequence)	NM_139329.1
Accession Version	NM_139329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Rattus norvegicus(Norway rat)
Product	3 beta-hydroxysteroid dehydrogenase type 7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB000199.1. ##Evidence-Data-START## Transcript exon combination :: AB000199.1, FM089357.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGACT CTGCACAGGT GCCAGCACTG GTATACCTGG TCACAGGTGG CTGTGGCTTC 
CTGGGGGAGC ATATTGTTCG AATGCTGCTG GAGTGGGAGC CCAGGCTCCG GGAGCTGCGT 
GTCTTTGACC TGCACCTGAG TTCTTGGCTG GAGGAGCTGA AAACAGGGCC TGTGCAGGTG 
ACTGCCATCC AGGGGGATGT GACTCAGGCC CACGAGGTGG CAGCAGCCAT GGCTGGATCT 
CATGTGGTCA TCCATACAGC TGGGCTGGTA GATGTGTTTG GGAAGGCCAG TCCAGAGACC 
ATCCACAAAG TCAATGTGCA GGGCACACAG AATGTGATTG ACGCTTGTGT GCAGACCGGG 
ACTCGGCTCC TGGTCTACAC GAGTAGCATG GAAGTGGTAG GGCCTAATGT CAAGGGCCAC 
CCCTTCTACA GGGGCAATGA AGACACCCCA TATGAGGCCA TCCACAGGCA TCCCTACCCA 
TGTAGCAAGG CCCTCGCTGA GCAGTTGGTC CTCGAGGCCA ATGGAAGGAA GGGACTGCGC 
TTTGGAGGTC GGCTGTTTCG GGCCATCCCA GCTTCCGTGG AGCATGGTCG GGTCTACGTT 
GGCAATGTGG CTTGGATGCA TATACTGGTG GCCCGAGAGC TGGAGCAGCG GGCAGCACTC 
ATGGGTGGCC AGGTGTATTT CTGCTATGAT AAGTCACCTT ATAAAAGCTA CGAGGACTTC 
AACATGGAGT TTCTGAGTCC CTGTGGACTT CGGCTGATAG GCACCCACCC ACTGCTGCCC 
TACTGGCTAC TGGTGCTGCT GACTGCCCTC AACGCTCTGC TGCAGTGGCT GCTTCGGCCG 
CTGGTGCTGT ACACACCCCT GCTGAATCCC TACACTCTGG CTGTGGCCAA CACCACCTTT 
ACTGTCAGCA CCAACAAGGC ACAGCGCCAT TTCGGCTACA AGCCCCTCTT CTCATGGGAA 
GAGAGCAGGG CCCGCACCAT TCACTGGGTG CAGGCCATGG AGGGTTCCGC CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647545.1
CDS	108..1124
Translation	MADSAQVPALVYLVTGGCGFLGEHIVRMLLEWEPRLRELRVFDLHLSSWLEELKTGPVQVTAIQGDVTQAHEVAAAMAGSHVVIHTAGLVDVFGKASPETIHKVNVQGTQNVIDACVQTGTRLLVYTSSMEVVGPNVKGHPFYRGNEDTPYEAIHRHPYPCSKALAEQLVLEANGRKGLRFGGRLFRAIPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLRLIGTHPLLPYWLLVLLTALNALLQWLLRPLVLYTPLLNPYTLAVANTTFTVSTNKAQRHFGYKPLFSWEESRARTIHWVQAMEGSAW

Target ORF information:

RefSeq Version	NM_139329.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139329.1

ORF Insert Sequence:

ATGGCAGACT CTGCACAGGT GCCAGCACTG GTATACCTGG TCACAGGTGG CTGTGGCTTC 
CTGGGGGAGC ATATTGTTCG AATGCTGCTG GAGTGGGAGC CCAGGCTCCG GGAGCTGCGT 
GTCTTTGACC TGCACCTGAG TTCTTGGCTG GAGGAGCTGA AAACAGGGCC TGTGCAGGTG 
ACTGCCATCC AGGGGGATGT GACTCAGGCC CACGAGGTGG CAGCAGCCAT GGCTGGATCT 
CATGTGGTCA TCCATACAGC TGGGCTGGTA GATGTGTTTG GGAAGGCCAG TCCAGAGACC 
ATCCACAAAG TCAATGTGCA GGGCACACAG AATGTGATTG ACGCTTGTGT GCAGACCGGG 
ACTCGGCTCC TGGTCTACAC GAGTAGCATG GAAGTGGTAG GGCCTAATGT CAAGGGCCAC 
CCCTTCTACA GGGGCAATGA AGACACCCCA TATGAGGCCA TCCACAGGCA TCCCTACCCA 
TGTAGCAAGG CCCTCGCTGA GCAGTTGGTC CTCGAGGCCA ATGGAAGGAA GGGACTGCGC 
TTTGGAGGTC GGCTGTTTCG GGCCATCCCA GCTTCCGTGG AGCATGGTCG GGTCTACGTT 
GGCAATGTGG CTTGGATGCA TATACTGGTG GCCCGAGAGC TGGAGCAGCG GGCAGCACTC 
ATGGGTGGCC AGGTGTATTT CTGCTATGAT AAGTCACCTT ATAAAAGCTA CGAGGACTTC 
AACATGGAGT TTCTGAGTCC CTGTGGACTT CGGCTGATAG GCACCCACCC ACTGCTGCCC 
TACTGGCTAC TGGTGCTGCT GACTGCCCTC AACGCTCTGC TGCAGTGGCT GCTTCGGCCG 
CTGGTGCTGT ACACACCCCT GCTGAATCCC TACACTCTGG CTGTGGCCAA CACCACCTTT 
ACTGTCAGCA CCAACAAGGC ACAGCGCCAT TTCGGCTACA AGCCCCTCTT CTCATGGGAA 
GAGAGCAGGG CCCGCACCAT TCACTGGGTG CAGGCCATGG AGGGTTCCGC CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsd3b7 ( XM_006230212.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230274.1 Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23402
Clone ID Related Accession (Same CDS sequence)	XM_006230212.3
Accession Version	XM_006230212.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230212.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACT CTGCACAGGT GCCAGCACTG GTATACCTGG TCACAGGTGG CTGTGGCTTC 
CTGGGGGAGC ATATTGTTCG AATGCTGCTG GAGTGGGAGC CCAGGCTCCG GGAGCTGCGT 
GTCTTTGACC TGCACCTGAG TTCTTGGCTG GAGGAGCTGA AAACAGGGCC TGTGCAGGTG 
ACTGCCATCC AGGGGGATGT GACTCAGGCC CACGAGGTGG CAGCAGCCAT GGCTGGATCT 
CATGTGGTCA TCCATACAGC TGGGCTGGTA GATGTGTTTG GGAAGGCCAG TCCAGAGACC 
ATCCACAAAG TCAATGTGCA GGGCACACAG AATGTGATTG ACGCTTGTGT GCAGACCGGG 
ACTCGGCTCC TGGTCTACAC GAGTAGCATG GAAGTGGTAG GGCCTAATGT CAAGGGCCAC 
CCCTTCTACA GGGGCAATGA AGACACCCCA TATGAGGCCA TCCACAGGCA TCCCTACCCA 
TGTAGCAAGG CCCTCGCTGA GCAGTTGGTC CTCGAGGCCA ATGGAAGGAA GGTCAATGGA 
GGGCTACCCC TGGTGACATG TGCCCTTCGA CCCACGGGCA TTTATGGTGA AGGTCATCAG 
GTCATGAGAG ACTTCTATCA CCAGGGACTG CGCTTTGGAG GTCGGCTGTT TCGGGCCATC 
CCAGCTTCCG TGGAGCATGG TCGGGTCTAC GTTGGCAATG TGGCTTGGAT GCATATACTG 
GTGGCCCGAG AGCTGGAGCA GCGGGCAGCA CTCATGGGTG GCCAGGTGTA TTTCTGCTAT 
GATAAGTCAC CTTATAAAAG CTACGAGGAC TTCAACATGG AGTTTCTGAG TCCCTGTGGA 
CTTCGGCTGA TAGGCACCCA CCCACTGCTG CCCTACTGGC TACTGGTGCT GCTGGCTGCC 
CTCAACGCTC TGCTGCAGTG GCTGCTTCGG CCGCTGGTGC TGTACACACC CCTGCTGAAT 
CCCTACACTC TGGCTGTGGC CAACACCACC TTTACTGTCA GCACCAACAA GGCACAGCGC 
CATTTCGGCT ACAAGCCCCT CTTCTCATGG GAAGAGAGCA GGGCCCGCAC CATTCACTGG 
GTGCAGGCCA TGGAGGGTTC CGCCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230274.1
CDS	494..1603
Translation	MADSAQVPALVYLVTGGCGFLGEHIVRMLLEWEPRLRELRVFDLHLSSWLEELKTGPVQVTAIQGDVTQAHEVAAAMAGSHVVIHTAGLVDVFGKASPETIHKVNVQGTQNVIDACVQTGTRLLVYTSSMEVVGPNVKGHPFYRGNEDTPYEAIHRHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYHQGLRFGGRLFRAIPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLRLIGTHPLLPYWLLVLLAALNALLQWLLRPLVLYTPLLNPYTLAVANTTFTVSTNKAQRHFGYKPLFSWEESRARTIHWVQAMEGSAW

Target ORF information:

RefSeq Version	XM_006230212.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230212.3

ORF Insert Sequence:

ATGGCAGACT CTGCACAGGT GCCAGCACTG GTATACCTGG TCACAGGTGG CTGTGGCTTC 
CTGGGGGAGC ATATTGTTCG AATGCTGCTG GAGTGGGAGC CCAGGCTCCG GGAGCTGCGT 
GTCTTTGACC TGCACCTGAG TTCTTGGCTG GAGGAGCTGA AAACAGGGCC TGTGCAGGTG 
ACTGCCATCC AGGGGGATGT GACTCAGGCC CACGAGGTGG CAGCAGCCAT GGCTGGATCT 
CATGTGGTCA TCCATACAGC TGGGCTGGTA GATGTGTTTG GGAAGGCCAG TCCAGAGACC 
ATCCACAAAG TCAATGTGCA GGGCACACAG AATGTGATTG ACGCTTGTGT GCAGACCGGG 
ACTCGGCTCC TGGTCTACAC GAGTAGCATG GAAGTGGTAG GGCCTAATGT CAAGGGCCAC 
CCCTTCTACA GGGGCAATGA AGACACCCCA TATGAGGCCA TCCACAGGCA TCCCTACCCA 
TGTAGCAAGG CCCTCGCTGA GCAGTTGGTC CTCGAGGCCA ATGGAAGGAA GGTCAATGGA 
GGGCTACCCC TGGTGACATG TGCCCTTCGA CCCACGGGCA TTTATGGTGA AGGTCATCAG 
GTCATGAGAG ACTTCTATCA CCAGGGACTG CGCTTTGGAG GTCGGCTGTT TCGGGCCATC 
CCAGCTTCCG TGGAGCATGG TCGGGTCTAC GTTGGCAATG TGGCTTGGAT GCATATACTG 
GTGGCCCGAG AGCTGGAGCA GCGGGCAGCA CTCATGGGTG GCCAGGTGTA TTTCTGCTAT 
GATAAGTCAC CTTATAAAAG CTACGAGGAC TTCAACATGG AGTTTCTGAG TCCCTGTGGA 
CTTCGGCTGA TAGGCACCCA CCCACTGCTG CCCTACTGGC TACTGGTGCT GCTGGCTGCC 
CTCAACGCTC TGCTGCAGTG GCTGCTTCGG CCGCTGGTGC TGTACACACC CCTGCTGAAT 
CCCTACACTC TGGCTGTGGC CAACACCACC TTTACTGTCA GCACCAACAA GGCACAGCGC 
CATTTCGGCT ACAAGCCCCT CTTCTCATGG GAAGAGAGCA GGGCCCGCAC CATTCACTGG 
GTGCAGGCCA TGGAGGGTTC CGCCTGGTGA