Magi3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Magi3
Entrez Gene ID	245903
Full Name	membrane associated guanylate kinase, WW and PDZ domain containing 3
Synonyms	Slipr
General protein information	Preferred Names membrane associated guanylate kinase, WW and PDZ domain containing 3 Names membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 MAGI-3 membrane-associated guanylate kinase inverted 3 membrane-associated guanylate kinase-related (MAGI-3) scaffolding protein SLIPR scaffolding-like protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	associates with tyrosine-phosphorylated proteins to link the receptor with its substrate in the plasma membrane [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006233058.2	XP_006233120.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform X1
XM_008761367.2	XP_008759589.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform X1
NM_139084.2	NP_620784.2	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Magi3	NP_620784.2
Canis lupus familiaris (dog)	MAGI3	XP_005630899.1
Gallus gallus (chicken)	MAGI3	NP_001012715.1
Macaca mulatta (Rhesus monkey)	LOC709341	XP_001097865.1
Bos taurus (cattle)	MAGI3	NP_001179675.1
Danio rerio (zebrafish)	LOC564220	XP_692662.4
Xenopus tropicalis (tropical clawed frog)	magi3	NP_001090712.1
Homo sapiens (human)	MAGI3	NP_001136254.1
Mus musculus (house mouse)	Magi3	NP_001152826.1
Danio rerio (zebrafish)	magi3	XP_690190.6

Gene Ontology
Bibliography

Related articles in PubMed

Proteomic analysis of beta1-adrenergic receptor interactions with PDZ scaffold proteins.
He J, Bellini M, Inuzuka H, Xu J, Xiong Y, Yang X, Castleberry AM, Hall RA
The Journal of biological chemistry281(5)2820-7(2006 Feb)

Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases.
Valiente M, Andrés-Pons A, Gomar B, Torres J, Gil A, Tapparel C, Antonarakis SE, Pulido R
The Journal of biological chemistry280(32)28936-43(2005 Aug)

Combined genealogical, mapping, and expression approaches to identify spontaneously hypertensive rat hypertension candidate genes.
Hinojos CA, Boerwinkle E, Fornage M, Doris PA
Hypertension (Dallas, Tex. : 1979)45(4)698-704(2005 Apr)

The complexity of PDZ domain-mediated interactions at glutamatergic synapses: a case study on neuroligin.
Meyer G, Varoqueaux F, Neeb A, Oschlies M, Brose N
Neuropharmacology47(5)724-33(2004 Oct)

MAGI-3 is involved in the regulation of the JNK signaling pathway as a scaffold protein for frizzled and Ltap.
Yao R, Natsume Y, Noda T
Oncogene23(36)6023-30(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Magi3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Magi3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13433	NM_139084.2 Latest version!	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa25124	XM_006233058.2 Latest version!	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa25124	XM_008761367.2 Latest version!	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Magi3 ( NM_139084.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620784.2 Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), mRNA.

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CloneID	ORa13433
Clone ID Related Accession (Same CDS sequence)	NM_139084.2
Accession Version	NM_139084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562256000000
Organism	Rattus norvegicus(Norway rat)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF255614.1 and CV112231.1. On Dec 19, 2008 this sequence version replaced NM_139084.1. ##Evidence-Data-START## Transcript exon combination :: AF255614.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCGAAGA CGTTGAAGAA GAAGAAGCAT TGGCTCAGCA AGGTGCAGGA GTGTGCGGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTGGGC GCTGAGATCC GCGGCGGCGC GGAGCGCGGC 
GAGTTCCCTT ACCTGGGGCG GCTCCGTGAC GAGCCTGGCG GCGGCGGAGG CACCTGCTGC 
GTGGTCTCGG GCAAGGCGCC CAGTCCTGGC GATGTGCTGT TGGAGGTGAA CGGGACGCCC 
GTCAGTGGGC TCACCAACCG GGACACCCTG GCTGTCATCC GCCACTTCCG CGAGCCAATC 
CGTCTGAAGA CCGTGAAGCC AGGCAAAGTC ATAAATAAAG ATTTGCGACA TTACCTGAGT 
CTGCAGTTTC AGAAAGGATC GATTGACCAC AAACTACAGC AGGTCATCAG AGATAACCTC 
TACTTGATAA CCATCCCATG CACTACAAGG GCTCCCAGGG ATGGGGAAGT TCCAGGGGTG 
GACTATAATT TCATTTCTGT TGAACAATTC AAGGCACTGG AAGAAAGCGG AGCATTGTTA 
GAAAGTGGAA CGTATGATGG AAATTTCTAT GGCACGCCCA AGCCTCCAGC AGAGCCTAGT 
CCTTTCCAGC CCGATCCAGT TGATCAGGTC CTCTTTGACA ATGAGTTTGA CACAGAATCC 
CAAAGAAAAC GAACTACATC TGTCAGCAAG ATGGAAAGGA TGGACAGCTC TCTCCCTGAG 
GAAGAGGAAG ATGAGGACAA AGAAGCTGTC AATGGCAGCG GAAGCATGGA AACTAGAGAG 
ATGCATTCTG AGTCGTCTGA CTGCTGGATG AAGACTGTTC CAAGTTATAA CCAAACAAAT 
CGCTCCATGG ACTTTAGAAA TTATATGATG AGAGACGAGA ATCTGGAGCC GCTGCCCAAA 
AACTGGGAAA TGGCCTACAC TGACACAGGA ACGATCTACT TCATTGATCA CAATACGAAG 
ACAACCACCT GGCTGGATCC TCGTCTCTGC AAGAAAGCCA AAGCCCCTGA AGACTGTGAG 
GATGGAGAGC TTCCATATGG CTGGGAGAAA ATAGAGGACC CTCAGTATGG AACATACTAT 
GTTGATCACC TCAACCAGAA AACTCAGTTT GAAAATCCAG TGGAGGAAGC GAAAAGGAAA 
AAGCAGATAG GACAGGCTGA AACTCATTCC GCAAAAACAG ATGTGGAAAG AGCACACTTT 
ACCCGGGACC CATCCCAACT TAAAGGTGTC CTTGTTCGAG CATCACTGAA AAAAAGCACA 
ATGGGCTTTG GTTTCACCAT TATAGGTGGA GATCGGCCTG ATGAATTTCT ACAAGTAAAA 
AATGTGCTCA AAGACGGTCC CGCTGCTCAG GATGGGAAAA TGGCACCAGG TGATGTTATT 
GTAGACATCA ATGGCAACTG TGTCCTTGGT CACACCCATG CAGATGTTGT CCAGATGTTT 
CAATTGGTAC CTGTCAACCA GTATGTCAAC CTTACTTTAT GCCGTGGTTA TGCACTTCCT 
GACGACAGTG AAGACCCTGT TGTGGACATT GTTGCTGCTA CCCCTGTTAT CAATGGACAA 
TCATTAGCAA AGGGAGAGGC GTGCATGAGT ACTCAGGATT TTAAACTGGG AGCAATGGTT 
TTGGATCAGA ATGGAAAATC AGGAAAACTT TTGTCCAGTG ATCGTCTCAA TGGCCCATCA 
GATTCAAATG AGCAAAGAGC ATCCCTGGCT TCATCAGGCA GCTCCCAGCC TGAACTAGTG 
ACTATCCCGC TGGTTAAGGG TCCCAAAGGC TTCGGGTTTG CAATTGCCGA TAGCCCAACT 
GGGCAGAAGG TGAAAATGAT ACTGGATAGC CAGTGGTGTC AAGGCCTTCA AAAAGGGGAT 
ATCATCAAAG AAATTTACCA TCAGAATGTG CAGAATTTAA CACATCTCCA AGTCGTAGAA 
GTACTGAAAC AGTTCCCAGT AGGTGCAGAT GTCCCGTTGC TTATCTTAAG AGGAGGTCCT 
TGTTCACCAA CTAAAACTGC CAAAATGAAA ACAGATACAA AAGAAACTTC AGGAAGTTTG 
GAGACTATAA ATGAGCCTAC TCCCCAGCCT ATGCCTTTTC CACCCAGCAT AATCAGATCA 
GGATCCCCGA AATTGGATCC TTCTGAAGTC TACCTGAAAT CTAAGACTTT ATATGAAGAT 
AAACCACCCA ACACCAAAGA CCTAGATGTC TTTCTTCGGA AACAAGAATC GGGGTTTGGC 
TTCAGGGTGC TGGGAGGAGA TGGACCTGAC CAGTCTATAT ATATTGGGGC CATCATTCCC 
CTGGGAGCAG CGGAAAAGGA TGGTAGGCTC CGTGCTGCTG ATGAATTGAT GTGTATTGAT 
GGGATTCCTG TTAAAGGGAA ATCACATAAG CAAGTCTTGG ACTTAATGAC CACTGCTGCT 
CGAAATGGCC ACGTGTTACT CACTGTGAGG AGGAAGATCT TCTATGGAGA GAAACAACCC 
GAGGATGAAA GCCCTCAAGC CTTCTCACAG AGTGGATCTC CTCGCCTAAA TCGGACAGAG 
CTTCCAACCA GGTCTGCCCC ACAGGAGTCC TATGATGTTA TCTTACAGAG GAAAGAAAAT 
GAAGGGTTTG GTTTTGTCAT CCTCACCTCT AAAAGCAAGC CGCCCCCAGG AGTTATTCCT 
CATAAAATTG GCCGGGTCAT AGATGGAAGT CCAGCTGATC GTTGTGGAAG ACTGAAAGTT 
GGAGACCACA TCTCTGCGGT GAATGGGCAG TCCATTGTTG ACCTGTCCCA TGATAATATT 
GTTCAGCTCA TCAAAGATGC TGGGGTCACC GTCACGCTGA CAGTGGTTGC TGAAGAAGAG 
CATCATGGTC CACCATCAGG AACAAACTCA GCCAGGCAGA GCCCAGCACT GCAGCACAGA 
CCCATGGGAC AGGCGCAGGC CACCCACATA CCTGGGGACA GAACTGCCCT AGAAGGTGAA 
GTTGGGAAAG ATGTCTGCAG CTCTTACAGA CACTCCTGGT CTGACCACAA GCACCTTGCA 
CAGCCGGACA CCGCGGTGAT TTCCGTGGTG GGCAGTCGGC ACAGTCAGAG CCTTGGGTGT 
TACCCTGTGG AGCTGGAGAG AGGCCCTCGA GGCTTTGGGT TCAGCCTCCG AGGAGGGAAG 
GAGTACAACA TGGGGCTGTT CATCCTTCGC CTTGCTGAGG ACGGGCCTGC CATCAAAGAC 
GGCAGAATTC ACGTTGGTGA CCAGATCGTG GAAATCAATG GGGAACCTAC ACAAGGCATC 
ACACACACGC GAGCCATTGA GCTCATTCAG GCTGGCGGGA ATAAAGTCCT CCTCCTTCTG 
AGGCCAGGAA CTGGCTTGAT ACCTGATCAT GGTGATTGGG ATATTTATAG TCCTTCATCT 
TCCAATGTCA TTTATGATGA ACAGCCACCA CCTCTCCCCT CTTCACATTC TGCTGCCACA 
TTTGAAGAGT CTCATGTGCC AGTAACTGAA GACTCTCTGA TTAGAGTTCA GACATGTGAA 
AAGGCAGAAG AATTAAAGGA CACTGTACAA GAAAAGAAAA GCACTTTAAA TGGAAGCCAG 
CCTGAGATGA AGTATCAGTC TGTCCAGAAA AATGTGAGTA AGAAGGATCC ATCCAGAAGT 
CATGGGCATG GTGACAAGAA TCTACCTAAA GGAGAAAATG GTGTTACACG GAGAGGTAGA 
TCTGCTAGTC CCAAAAAGTC AGTTAATCGA CATTCAGAGG AACATCTGGA AAAGATTCCC 
AGGCCCCTTA GAAGTGACCC CAAAGGAAAA TCTAGAGACC GGTCACTCAG CCCTAGGAAA 
GGAGAAAATA AAGGTCAGGT CACCATTAAG GCGGGCTCTG GACAAGATCC CTGTAGAAAA 
GACAGAGGAC GGTCTTCTAG CCCCAGGAAG CAGCAAAAGA TTGGAGGCAA CAGCCTGTCA 
AACACTGAAG GCAAATTATC AGAGGCTGGG AGCCGAAGAG CAGCAGGCCT CTCTAGTGAC 
AGCCCTGAAC AGCTCCCAGA GGGGAAAGAA AAGTCAGGGG TCAGCAGGAA AGATCTGAAG 
CTCAGTCAGC TGGGGAAAAA CAGAACAAGG TCTCCAGAGA AAAGGAGCAG CAAAGTTGAT 
GAAGCGTCTC TCCCATCCAA AAAGACTAGT GACACTGCTA GTAGGGTAGT GTCTGAAAAG 
GAGAAAGGAA GGAAGCCAGG CACAGGAGAA AGAAGTAGAG ACAAAACAGG AGAAAGCGTC 
CAGACCTCAG CAAAGCCGCT CACGCAGGAA GCGGGAGAGA AGATGGCATT AAGCAAAGCA 
TCAGAGGTCA CTGACAGAGG CAAGGAGCGT GCAGGGGGCG CACCTGAGAG TAGCTCTCCA 
GTCAAGAAAG CACCCATCAC TCCAGGACCC TGGAGGGTGC CACGTGCAAA TAAAGTCACA 
GGCACTGCTG GCATGGCTGA CAAACAGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620784.2
CDS	128..4540
Translation	MSKTLKKKKHWLSKVQECAVSWAGPPGDLGAEIRGGAERGEFPYLGRLRDEPGGGGGTCCVVSGKAPSPGDVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNLYLITIPCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDNEFDTESQRKRTTSVSKMERMDSSLPEEEEDEDKEAVNGSGSMETREMHSESSDCWMKTVPSYNQTNRSMDFRNYMMRDENLEPLPKNWEMAYTDTGTIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQIGQAETHSAKTDVERAHFTRDPSQLKGVLVRASLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKMAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYALPDDSEDPVVDIVAATPVINGQSLAKGEACMSTQDFKLGAMVLDQNGKSGKLLSSDRLNGPSDSNEQRASLASSGSSQPELVTIPLVKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPCSPTKTAKMKTDTKETSGSLETINEPTPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDESPQAFSQSGSPRLNRTELPTRSAPQESYDVILQRKENEGFGFVILTSKSKPPPGVIPHKIGRVIDGSPADRCGRLKVGDHISAVNGQSIVDLSHDNIVQLIKDAGVTVTLTVVAEEEHHGPPSGTNSARQSPALQHRPMGQAQATHIPGDRTALEGEVGKDVCSSYRHSWSDHKHLAQPDTAVISVVGSRHSQSLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGDWDIYSPSSSNVIYDEQPPPLPSSHSAATFEESHVPVTEDSLIRVQTCEKAEELKDTVQEKKSTLNGSQPEMKYQSVQKNVSKKDPSRSHGHGDKNLPKGENGVTRRGRSASPKKSVNRHSEEHLEKIPRPLRSDPKGKSRDRSLSPRKGENKGQVTIKAGSGQDPCRKDRGRSSSPRKQQKIGGNSLSNTEGKLSEAGSRRAAGLSSDSPEQLPEGKEKSGVSRKDLKLSQLGKNRTRSPEKRSSKVDEASLPSKKTSDTASRVVSEKEKGRKPGTGERSRDKTGESVQTSAKPLTQEAGEKMALSKASEVTDRGKERAGGAPESSSPVKKAPITPGPWRVPRANKVTGTAGMADKQL

Target ORF information:

RefSeq Version	NM_139084.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139084.2

ORF Insert Sequence:

ATGTCGAAGA CGTTGAAGAA GAAGAAGCAT TGGCTCAGCA AGGTGCAGGA GTGTGCGGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTGGGC GCTGAGATCC GCGGCGGCGC GGAGCGCGGC 
GAGTTCCCTT ACCTGGGGCG GCTCCGTGAC GAGCCTGGCG GCGGCGGAGG CACCTGCTGC 
GTGGTCTCGG GCAAGGCGCC CAGTCCTGGC GATGTGCTGT TGGAGGTGAA CGGGACGCCC 
GTCAGTGGGC TCACCAACCG GGACACCCTG GCTGTCATCC GCCACTTCCG CGAGCCAATC 
CGTCTGAAGA CCGTGAAGCC AGGCAAAGTC ATAAATAAAG ATTTGCGACA TTACCTGAGT 
CTGCAGTTTC AGAAAGGATC GATTGACCAC AAACTACAGC AGGTCATCAG AGATAACCTC 
TACTTGATAA CCATCCCATG CACTACAAGG GCTCCCAGGG ATGGGGAAGT TCCAGGGGTG 
GACTATAATT TCATTTCTGT TGAACAATTC AAGGCACTGG AAGAAAGCGG AGCATTGTTA 
GAAAGTGGAA CGTATGATGG AAATTTCTAT GGCACGCCCA AGCCTCCAGC AGAGCCTAGT 
CCTTTCCAGC CCGATCCAGT TGATCAGGTC CTCTTTGACA ATGAGTTTGA CACAGAATCC 
CAAAGAAAAC GAACTACATC TGTCAGCAAG ATGGAAAGGA TGGACAGCTC TCTCCCTGAG 
GAAGAGGAAG ATGAGGACAA AGAAGCTGTC AATGGCAGCG GAAGCATGGA AACTAGAGAG 
ATGCATTCTG AGTCGTCTGA CTGCTGGATG AAGACTGTTC CAAGTTATAA CCAAACAAAT 
CGCTCCATGG ACTTTAGAAA TTATATGATG AGAGACGAGA ATCTGGAGCC GCTGCCCAAA 
AACTGGGAAA TGGCCTACAC TGACACAGGA ACGATCTACT TCATTGATCA CAATACGAAG 
ACAACCACCT GGCTGGATCC TCGTCTCTGC AAGAAAGCCA AAGCCCCTGA AGACTGTGAG 
GATGGAGAGC TTCCATATGG CTGGGAGAAA ATAGAGGACC CTCAGTATGG AACATACTAT 
GTTGATCACC TCAACCAGAA AACTCAGTTT GAAAATCCAG TGGAGGAAGC GAAAAGGAAA 
AAGCAGATAG GACAGGCTGA AACTCATTCC GCAAAAACAG ATGTGGAAAG AGCACACTTT 
ACCCGGGACC CATCCCAACT TAAAGGTGTC CTTGTTCGAG CATCACTGAA AAAAAGCACA 
ATGGGCTTTG GTTTCACCAT TATAGGTGGA GATCGGCCTG ATGAATTTCT ACAAGTAAAA 
AATGTGCTCA AAGACGGTCC CGCTGCTCAG GATGGGAAAA TGGCACCAGG TGATGTTATT 
GTAGACATCA ATGGCAACTG TGTCCTTGGT CACACCCATG CAGATGTTGT CCAGATGTTT 
CAATTGGTAC CTGTCAACCA GTATGTCAAC CTTACTTTAT GCCGTGGTTA TGCACTTCCT 
GACGACAGTG AAGACCCTGT TGTGGACATT GTTGCTGCTA CCCCTGTTAT CAATGGACAA 
TCATTAGCAA AGGGAGAGGC GTGCATGAGT ACTCAGGATT TTAAACTGGG AGCAATGGTT 
TTGGATCAGA ATGGAAAATC AGGAAAACTT TTGTCCAGTG ATCGTCTCAA TGGCCCATCA 
GATTCAAATG AGCAAAGAGC ATCCCTGGCT TCATCAGGCA GCTCCCAGCC TGAACTAGTG 
ACTATCCCGC TGGTTAAGGG TCCCAAAGGC TTCGGGTTTG CAATTGCCGA TAGCCCAACT 
GGGCAGAAGG TGAAAATGAT ACTGGATAGC CAGTGGTGTC AAGGCCTTCA AAAAGGGGAT 
ATCATCAAAG AAATTTACCA TCAGAATGTG CAGAATTTAA CACATCTCCA AGTCGTAGAA 
GTACTGAAAC AGTTCCCAGT AGGTGCAGAT GTCCCGTTGC TTATCTTAAG AGGAGGTCCT 
TGTTCACCAA CTAAAACTGC CAAAATGAAA ACAGATACAA AAGAAACTTC AGGAAGTTTG 
GAGACTATAA ATGAGCCTAC TCCCCAGCCT ATGCCTTTTC CACCCAGCAT AATCAGATCA 
GGATCCCCGA AATTGGATCC TTCTGAAGTC TACCTGAAAT CTAAGACTTT ATATGAAGAT 
AAACCACCCA ACACCAAAGA CCTAGATGTC TTTCTTCGGA AACAAGAATC GGGGTTTGGC 
TTCAGGGTGC TGGGAGGAGA TGGACCTGAC CAGTCTATAT ATATTGGGGC CATCATTCCC 
CTGGGAGCAG CGGAAAAGGA TGGTAGGCTC CGTGCTGCTG ATGAATTGAT GTGTATTGAT 
GGGATTCCTG TTAAAGGGAA ATCACATAAG CAAGTCTTGG ACTTAATGAC CACTGCTGCT 
CGAAATGGCC ACGTGTTACT CACTGTGAGG AGGAAGATCT TCTATGGAGA GAAACAACCC 
GAGGATGAAA GCCCTCAAGC CTTCTCACAG AGTGGATCTC CTCGCCTAAA TCGGACAGAG 
CTTCCAACCA GGTCTGCCCC ACAGGAGTCC TATGATGTTA TCTTACAGAG GAAAGAAAAT 
GAAGGGTTTG GTTTTGTCAT CCTCACCTCT AAAAGCAAGC CGCCCCCAGG AGTTATTCCT 
CATAAAATTG GCCGGGTCAT AGATGGAAGT CCAGCTGATC GTTGTGGAAG ACTGAAAGTT 
GGAGACCACA TCTCTGCGGT GAATGGGCAG TCCATTGTTG ACCTGTCCCA TGATAATATT 
GTTCAGCTCA TCAAAGATGC TGGGGTCACC GTCACGCTGA CAGTGGTTGC TGAAGAAGAG 
CATCATGGTC CACCATCAGG AACAAACTCA GCCAGGCAGA GCCCAGCACT GCAGCACAGA 
CCCATGGGAC AGGCGCAGGC CACCCACATA CCTGGGGACA GAACTGCCCT AGAAGGTGAA 
GTTGGGAAAG ATGTCTGCAG CTCTTACAGA CACTCCTGGT CTGACCACAA GCACCTTGCA 
CAGCCGGACA CCGCGGTGAT TTCCGTGGTG GGCAGTCGGC ACAGTCAGAG CCTTGGGTGT 
TACCCTGTGG AGCTGGAGAG AGGCCCTCGA GGCTTTGGGT TCAGCCTCCG AGGAGGGAAG 
GAGTACAACA TGGGGCTGTT CATCCTTCGC CTTGCTGAGG ACGGGCCTGC CATCAAAGAC 
GGCAGAATTC ACGTTGGTGA CCAGATCGTG GAAATCAATG GGGAACCTAC ACAAGGCATC 
ACACACACGC GAGCCATTGA GCTCATTCAG GCTGGCGGGA ATAAAGTCCT CCTCCTTCTG 
AGGCCAGGAA CTGGCTTGAT ACCTGATCAT GGTGATTGGG ATATTTATAG TCCTTCATCT 
TCCAATGTCA TTTATGATGA ACAGCCACCA CCTCTCCCCT CTTCACATTC TGCTGCCACA 
TTTGAAGAGT CTCATGTGCC AGTAACTGAA GACTCTCTGA TTAGAGTTCA GACATGTGAA 
AAGGCAGAAG AATTAAAGGA CACTGTACAA GAAAAGAAAA GCACTTTAAA TGGAAGCCAG 
CCTGAGATGA AGTATCAGTC TGTCCAGAAA AATGTGAGTA AGAAGGATCC ATCCAGAAGT 
CATGGGCATG GTGACAAGAA TCTACCTAAA GGAGAAAATG GTGTTACACG GAGAGGTAGA 
TCTGCTAGTC CCAAAAAGTC AGTTAATCGA CATTCAGAGG AACATCTGGA AAAGATTCCC 
AGGCCCCTTA GAAGTGACCC CAAAGGAAAA TCTAGAGACC GGTCACTCAG CCCTAGGAAA 
GGAGAAAATA AAGGTCAGGT CACCATTAAG GCGGGCTCTG GACAAGATCC CTGTAGAAAA 
GACAGAGGAC GGTCTTCTAG CCCCAGGAAG CAGCAAAAGA TTGGAGGCAA CAGCCTGTCA 
AACACTGAAG GCAAATTATC AGAGGCTGGG AGCCGAAGAG CAGCAGGCCT CTCTAGTGAC 
AGCCCTGAAC AGCTCCCAGA GGGGAAAGAA AAGTCAGGGG TCAGCAGGAA AGATCTGAAG 
CTCAGTCAGC TGGGGAAAAA CAGAACAAGG TCTCCAGAGA AAAGGAGCAG CAAAGTTGAT 
GAAGCGTCTC TCCCATCCAA AAAGACTAGT GACACTGCTA GTAGGGTAGT GTCTGAAAAG 
GAGAAAGGAA GGAAGCCAGG CACAGGAGAA AGAAGTAGAG ACAAAACAGG AGAAAGCGTC 
CAGACCTCAG CAAAGCCGCT CACGCAGGAA GCGGGAGAGA AGATGGCATT AAGCAAAGCA 
TCAGAGGTCA CTGACAGAGG CAAGGAGCGT GCAGGGGGCG CACCTGAGAG TAGCTCTCCA 
GTCAAGAAAG CACCCATCAC TCCAGGACCC TGGAGGGTGC CACGTGCAAA TAAAGTCACA 
GGCACTGCTG GCATGGCTGA CAAACAGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Magi3 ( XM_006233058.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233120.1 Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa25124
Clone ID Related Accession (Same CDS sequence)	XM_006233058.2 , XM_008761367.2
Accession Version	XM_006233058.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006233058.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAGA CGTTGAAGAA GAAGAAGCAT TGGCTCAGCA AGGTGCAGGA GTGTGCGGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTGGGC GCTGAGATCC GCGGCGGCGC GGAGCGCGGC 
GAGTTCCCTT ACCTGGGGCG GCTCCGTGAC GAGCCTGGCG GCGGCGGAGG CACCTGCTGC 
GTGGTCTCGG GCAAGGCGCC CAGTCCTGGC GATGTGCTGT TGGAGGTGAA CGGGACGCCC 
GTCAGTGGGC TCACCAACCG GGACACCCTG GCTGTCATCC GCCACTTCCG CGAGCCAATC 
CGTCTGAAGA CCGTGAAGCC AGGCAAAGTC ATAAATAAAG ATTTGCGACA TTACCTGAGT 
CTGCAGTTTC AGAAAGGATC GATTGACCAC AAACTACAGC AGGTCATCAG AGATAACCTC 
TACTTGAGAA CCATCCCATG CACTACAAGG GCTCCCAGGG ATGGGGAAGT TCCAGGGGTG 
GACTATAATT TCATTTCTGT TGAACAATTC AAGGCACTGG AAGAAAGCGG AGCATTGTTA 
GAAAGTGGAA CGTATGATGG AAATTTCTAT GGCACGCCCA AGCCTCCAGC AGAGCCTAGT 
CCTTTCCAGC CCGATCCAGT TGATCAGGTC CTCTTTGACA ATGAGTTTGA CACAGAATCC 
CAAAGAAAAC GAACTACATC TGTCAGCAAG ATGGAAAGGA TGGACAGCTC TCTCCCTGAG 
GAAGAGGAAG ATGAGGACAA AGAAGCTGTC AATGGCAGCG GAAGCATGGA AACTAGAGAG 
ATGCATTCTG AGTCGTCTGA CTGCTGGATG AAGACTGTTC CAAGTTATAA CCAAACAAAT 
CGCTCCATGG ACTTTAGAAA TTATATGATG AGAGACGAGA ATCTGGAGCC GCTGCCCAAA 
AACTGGGAAA TGGCCTACAC TGACACAGGA ACGATCTACT TCATTGATCA CAATACGAAG 
ACAACCACCT GGCTGGATCC TCGTCTCTGC AAGAAAGCCA AAGCCCCTGA AGACTGTGAG 
GATGGAGAGC TTCCATATGG CTGGGAGAAA ATAGAGGACC CTCAGTATGG AACATACTAT 
GTTGATCACC TCAACCAGAA AACTCAGTTT GAAAATCCAG TGGAGGAAGC GAAAAGGAAA 
AAGCAGATAG GACAGGCTGA AACTCATTCC GCAAAAACAG ATGTGGAAAG AGCACACTTT 
ACCCGGGACC CATCCCAACT TAAAGGTGTC CTTGTTCGAG CATCACTGAA AAAAAGCACA 
ATGGGCTTTG GTTTCACCAT TATAGGTGGA GATCGGCCTG ATGAATTTCT ACAAGTAAAA 
AATGTGCTCA AAGACGGTCC CGCTGCTCAG GATGGGAAAA TGGCACCAGG TGATGTTATT 
GTAGACATCA ATGGCAACTG TGTCCTTGGT CACACCCATG CAGATGTTGT CCAGATGTTT 
CAATTGGTAC CTGTCAACCA GTATGTCAAC CTTACTTTAT GCCGTGGTTA TGCACTTCCT 
GACGACAGTG AAGACCCTGT TGTGGACATT GTTGCTGCTA CCCCTGTTAT CAATGGACAA 
TCATTAGCAA AGGGAGAGGC GTGCATGAGT ACTCAGGATT TTAAACTGGG AGCAATGGTT 
TTGGATCAGA ATGGAAAATC AGGAAAACTT TTGTCCAGTG ATCATCTCAA TGGCCCATCA 
GATTCAAATG AGCAAAGAGC ATCCCTGGCT TCATCAGGCA GCTCCCAGCC TGAACTAGTG 
ACTATCCCGC TGGTTAAGGG TCCCAAAGGC TTCGGGTTTG CAATTGCCGA TAGCCCAACT 
GGGCAGAAGG TGAAAATGAT ACTGGATAGC CAGTGGTGTC AAGGCCTTCA AAAAGGGGAT 
ATCATCAAAG AAATTTACCA TCAGAATGTG CAGAATTTAA CACATCTCCA AGTCGTAGAA 
GTACTGAAAC AGTTCCCAGT AGGTGCAGAT GTCCCGTTGC TTATCTTAAG AGGAGGTCCT 
TGTTCACCAA CTAAAACTGC CAAAATGAAA ACAGATACAA AAGAAACTTC AGGAAGTTTG 
GAGACTATAA ATGAGCCTAC TCCCCAGCCT ATGCCTTTTC CACCCAGCAT AATCAGATCA 
GGATCCCCGA AATTGGATCC TTCTGAAGTC TACCTGAAAT CTAAGACTTT ATATGAAGAT 
AAACCACCCA ACACCAAAGA CCTAGATGTC TTTCTTCGGA AACAAGAATC GGGGTTTGGC 
TTCAGGGTGC TGGGAGGAGA TGGACCTGAC CAGTCTATAT ATATTGGGGC CATCATTCCC 
CTGGGAGCAG CGGAAAAGGA TGGTAGGCTC CGTGCTGCTG ATGAATTGAT GTGTATTGAT 
GGGATTCCTG TTAAAGGGAA ATCACATAAG CAAGTCTTGG ACTTAATGAC CACTGCTGCT 
CGAAATGGCC ACGTGTTACT CACTGTGAGG AGGAAGATCT TCTATGGAGA GAAACAACCC 
GAGGATGAAA GCCCTCAAGC CTTCTCACAG AGTGGATCTC CTCGCCTAAA TCGGACAGAG 
CTTCCAACCA GGTCTGCCCC ACAGGAGTCC TATGATGTTA TCTTACAGAG GAAAGAAAAT 
GAAGGGTTTG GTTTTGTCAT CCTCACCTCT AAAAGCAAGC CGCCCCCAGG AGTTATTCCT 
CATAAAATTG GCCGGGTCAT AGATGGAAGT CCAGCTGATC GTTGTGGAAG ACTGAAAGTT 
GGAGACCACA TCTCTGCGGT GAATGGGCAG TCCATTGTTG ACCTGTCCCA TGATAATATT 
GTTCAGCTCA TCAAAGATGC TGGGGTCACC GTCACGCTGA CAGTGGTTGC TGAAGAAGAG 
CATCATGGTC CACCATCAGG AACAAACTCA GCCAGGCAGA GCCCAGCACT GCAGCACAGA 
CCCATGGGAC AGGCGCAGGC CACCCACATA CCTGGGGACA GAACTGCCCT AGAAGGTGAA 
GTTGGGAAAG ATGTCTGCAG CTCTTACAGA CACTCCTGGT CTGACCACAA GCACCTTGCA 
CAGCCGGACA CCGCGGTGAT TTCCGTGGTG GGCAGTCGGC ACAGTCAGAG CCTTGGGTGT 
TACCCTGTGG AGCTGGAGAG AGGCCCTCGA GGCTTTGGGT TCAGCCTCCG AGGAGGGAAG 
GAGTACAACA TGGGGCTGTT CATCCTTCGC CTTGCTGAGG ACGGGCCTGC CATCAAAGAC 
GGCAGAATTC ACGTTGGTGA CCAGATCGTG GAAATCAATG GGGAACCTAC GCAAGGCATC 
ACACACACGC GAGCCATTGA GCTCATTCAG GCTGGCGGGA ATAAAGTCCT CCTCCTTCTG 
AGGCCAGGAA CTGGCTTGAT ACCTGATCAT GGTTTGGCTC CTTCCGGTCT GTGCTCCTTC 
GTGAAACCTG AGCAACATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233120.1
CDS	116..3496
Translation	MSKTLKKKKHWLSKVQECAVSWAGPPGDLGAEIRGGAERGEFPYLGRLRDEPGGGGGTCCVVSGKAPSPGDVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNLYLRTIPCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDNEFDTESQRKRTTSVSKMERMDSSLPEEEEDEDKEAVNGSGSMETREMHSESSDCWMKTVPSYNQTNRSMDFRNYMMRDENLEPLPKNWEMAYTDTGTIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQIGQAETHSAKTDVERAHFTRDPSQLKGVLVRASLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKMAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYALPDDSEDPVVDIVAATPVINGQSLAKGEACMSTQDFKLGAMVLDQNGKSGKLLSSDHLNGPSDSNEQRASLASSGSSQPELVTIPLVKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPCSPTKTAKMKTDTKETSGSLETINEPTPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDESPQAFSQSGSPRLNRTELPTRSAPQESYDVILQRKENEGFGFVILTSKSKPPPGVIPHKIGRVIDGSPADRCGRLKVGDHISAVNGQSIVDLSHDNIVQLIKDAGVTVTLTVVAEEEHHGPPSGTNSARQSPALQHRPMGQAQATHIPGDRTALEGEVGKDVCSSYRHSWSDHKHLAQPDTAVISVVGSRHSQSLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGLAPSGLCSFVKPEQH

Target ORF information:

RefSeq Version	XM_006233058.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233058.2

ORF Insert Sequence:

ATGTCGAAGA CGTTGAAGAA GAAGAAGCAT TGGCTCAGCA AGGTGCAGGA GTGTGCGGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTGGGC GCTGAGATCC GCGGCGGCGC GGAGCGCGGC 
GAGTTCCCTT ACCTGGGGCG GCTCCGTGAC GAGCCTGGCG GCGGCGGAGG CACCTGCTGC 
GTGGTCTCGG GCAAGGCGCC CAGTCCTGGC GATGTGCTGT TGGAGGTGAA CGGGACGCCC 
GTCAGTGGGC TCACCAACCG GGACACCCTG GCTGTCATCC GCCACTTCCG CGAGCCAATC 
CGTCTGAAGA CCGTGAAGCC AGGCAAAGTC ATAAATAAAG ATTTGCGACA TTACCTGAGT 
CTGCAGTTTC AGAAAGGATC GATTGACCAC AAACTACAGC AGGTCATCAG AGATAACCTC 
TACTTGAGAA CCATCCCATG CACTACAAGG GCTCCCAGGG ATGGGGAAGT TCCAGGGGTG 
GACTATAATT TCATTTCTGT TGAACAATTC AAGGCACTGG AAGAAAGCGG AGCATTGTTA 
GAAAGTGGAA CGTATGATGG AAATTTCTAT GGCACGCCCA AGCCTCCAGC AGAGCCTAGT 
CCTTTCCAGC CCGATCCAGT TGATCAGGTC CTCTTTGACA ATGAGTTTGA CACAGAATCC 
CAAAGAAAAC GAACTACATC TGTCAGCAAG ATGGAAAGGA TGGACAGCTC TCTCCCTGAG 
GAAGAGGAAG ATGAGGACAA AGAAGCTGTC AATGGCAGCG GAAGCATGGA AACTAGAGAG 
ATGCATTCTG AGTCGTCTGA CTGCTGGATG AAGACTGTTC CAAGTTATAA CCAAACAAAT 
CGCTCCATGG ACTTTAGAAA TTATATGATG AGAGACGAGA ATCTGGAGCC GCTGCCCAAA 
AACTGGGAAA TGGCCTACAC TGACACAGGA ACGATCTACT TCATTGATCA CAATACGAAG 
ACAACCACCT GGCTGGATCC TCGTCTCTGC AAGAAAGCCA AAGCCCCTGA AGACTGTGAG 
GATGGAGAGC TTCCATATGG CTGGGAGAAA ATAGAGGACC CTCAGTATGG AACATACTAT 
GTTGATCACC TCAACCAGAA AACTCAGTTT GAAAATCCAG TGGAGGAAGC GAAAAGGAAA 
AAGCAGATAG GACAGGCTGA AACTCATTCC GCAAAAACAG ATGTGGAAAG AGCACACTTT 
ACCCGGGACC CATCCCAACT TAAAGGTGTC CTTGTTCGAG CATCACTGAA AAAAAGCACA 
ATGGGCTTTG GTTTCACCAT TATAGGTGGA GATCGGCCTG ATGAATTTCT ACAAGTAAAA 
AATGTGCTCA AAGACGGTCC CGCTGCTCAG GATGGGAAAA TGGCACCAGG TGATGTTATT 
GTAGACATCA ATGGCAACTG TGTCCTTGGT CACACCCATG CAGATGTTGT CCAGATGTTT 
CAATTGGTAC CTGTCAACCA GTATGTCAAC CTTACTTTAT GCCGTGGTTA TGCACTTCCT 
GACGACAGTG AAGACCCTGT TGTGGACATT GTTGCTGCTA CCCCTGTTAT CAATGGACAA 
TCATTAGCAA AGGGAGAGGC GTGCATGAGT ACTCAGGATT TTAAACTGGG AGCAATGGTT 
TTGGATCAGA ATGGAAAATC AGGAAAACTT TTGTCCAGTG ATCATCTCAA TGGCCCATCA 
GATTCAAATG AGCAAAGAGC ATCCCTGGCT TCATCAGGCA GCTCCCAGCC TGAACTAGTG 
ACTATCCCGC TGGTTAAGGG TCCCAAAGGC TTCGGGTTTG CAATTGCCGA TAGCCCAACT 
GGGCAGAAGG TGAAAATGAT ACTGGATAGC CAGTGGTGTC AAGGCCTTCA AAAAGGGGAT 
ATCATCAAAG AAATTTACCA TCAGAATGTG CAGAATTTAA CACATCTCCA AGTCGTAGAA 
GTACTGAAAC AGTTCCCAGT AGGTGCAGAT GTCCCGTTGC TTATCTTAAG AGGAGGTCCT 
TGTTCACCAA CTAAAACTGC CAAAATGAAA ACAGATACAA AAGAAACTTC AGGAAGTTTG 
GAGACTATAA ATGAGCCTAC TCCCCAGCCT ATGCCTTTTC CACCCAGCAT AATCAGATCA 
GGATCCCCGA AATTGGATCC TTCTGAAGTC TACCTGAAAT CTAAGACTTT ATATGAAGAT 
AAACCACCCA ACACCAAAGA CCTAGATGTC TTTCTTCGGA AACAAGAATC GGGGTTTGGC 
TTCAGGGTGC TGGGAGGAGA TGGACCTGAC CAGTCTATAT ATATTGGGGC CATCATTCCC 
CTGGGAGCAG CGGAAAAGGA TGGTAGGCTC CGTGCTGCTG ATGAATTGAT GTGTATTGAT 
GGGATTCCTG TTAAAGGGAA ATCACATAAG CAAGTCTTGG ACTTAATGAC CACTGCTGCT 
CGAAATGGCC ACGTGTTACT CACTGTGAGG AGGAAGATCT TCTATGGAGA GAAACAACCC 
GAGGATGAAA GCCCTCAAGC CTTCTCACAG AGTGGATCTC CTCGCCTAAA TCGGACAGAG 
CTTCCAACCA GGTCTGCCCC ACAGGAGTCC TATGATGTTA TCTTACAGAG GAAAGAAAAT 
GAAGGGTTTG GTTTTGTCAT CCTCACCTCT AAAAGCAAGC CGCCCCCAGG AGTTATTCCT 
CATAAAATTG GCCGGGTCAT AGATGGAAGT CCAGCTGATC GTTGTGGAAG ACTGAAAGTT 
GGAGACCACA TCTCTGCGGT GAATGGGCAG TCCATTGTTG ACCTGTCCCA TGATAATATT 
GTTCAGCTCA TCAAAGATGC TGGGGTCACC GTCACGCTGA CAGTGGTTGC TGAAGAAGAG 
CATCATGGTC CACCATCAGG AACAAACTCA GCCAGGCAGA GCCCAGCACT GCAGCACAGA 
CCCATGGGAC AGGCGCAGGC CACCCACATA CCTGGGGACA GAACTGCCCT AGAAGGTGAA 
GTTGGGAAAG ATGTCTGCAG CTCTTACAGA CACTCCTGGT CTGACCACAA GCACCTTGCA 
CAGCCGGACA CCGCGGTGAT TTCCGTGGTG GGCAGTCGGC ACAGTCAGAG CCTTGGGTGT 
TACCCTGTGG AGCTGGAGAG AGGCCCTCGA GGCTTTGGGT TCAGCCTCCG AGGAGGGAAG 
GAGTACAACA TGGGGCTGTT CATCCTTCGC CTTGCTGAGG ACGGGCCTGC CATCAAAGAC 
GGCAGAATTC ACGTTGGTGA CCAGATCGTG GAAATCAATG GGGAACCTAC GCAAGGCATC 
ACACACACGC GAGCCATTGA GCTCATTCAG GCTGGCGGGA ATAAAGTCCT CCTCCTTCTG 
AGGCCAGGAA CTGGCTTGAT ACCTGATCAT GGTTTGGCTC CTTCCGGTCT GTGCTCCTTC 
GTGAAACCTG AGCAACATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Magi3 ( XM_008761367.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759589.1 Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), transcript variant X2, mRNA.

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CloneID	ORa25124
Clone ID Related Accession (Same CDS sequence)	XM_006233058.2 , XM_008761367.2
Accession Version	XM_008761367.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761367.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAGA CGTTGAAGAA GAAGAAGCAT TGGCTCAGCA AGGTGCAGGA GTGTGCGGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTGGGC GCTGAGATCC GCGGCGGCGC GGAGCGCGGC 
GAGTTCCCTT ACCTGGGGCG GCTCCGTGAC GAGCCTGGCG GCGGCGGAGG CACCTGCTGC 
GTGGTCTCGG GCAAGGCGCC CAGTCCTGGC GATGTGCTGT TGGAGGTGAA CGGGACGCCC 
GTCAGTGGGC TCACCAACCG GGACACCCTG GCTGTCATCC GCCACTTCCG CGAGCCAATC 
CGTCTGAAGA CCGTGAAGCC AGGCAAAGTC ATAAATAAAG ATTTGCGACA TTACCTGAGT 
CTGCAGTTTC AGAAAGGATC GATTGACCAC AAACTACAGC AGGTCATCAG AGATAACCTC 
TACTTGAGAA CCATCCCATG CACTACAAGG GCTCCCAGGG ATGGGGAAGT TCCAGGGGTG 
GACTATAATT TCATTTCTGT TGAACAATTC AAGGCACTGG AAGAAAGCGG AGCATTGTTA 
GAAAGTGGAA CGTATGATGG AAATTTCTAT GGCACGCCCA AGCCTCCAGC AGAGCCTAGT 
CCTTTCCAGC CCGATCCAGT TGATCAGGTC CTCTTTGACA ATGAGTTTGA CACAGAATCC 
CAAAGAAAAC GAACTACATC TGTCAGCAAG ATGGAAAGGA TGGACAGCTC TCTCCCTGAG 
GAAGAGGAAG ATGAGGACAA AGAAGCTGTC AATGGCAGCG GAAGCATGGA AACTAGAGAG 
ATGCATTCTG AGTCGTCTGA CTGCTGGATG AAGACTGTTC CAAGTTATAA CCAAACAAAT 
CGCTCCATGG ACTTTAGAAA TTATATGATG AGAGACGAGA ATCTGGAGCC GCTGCCCAAA 
AACTGGGAAA TGGCCTACAC TGACACAGGA ACGATCTACT TCATTGATCA CAATACGAAG 
ACAACCACCT GGCTGGATCC TCGTCTCTGC AAGAAAGCCA AAGCCCCTGA AGACTGTGAG 
GATGGAGAGC TTCCATATGG CTGGGAGAAA ATAGAGGACC CTCAGTATGG AACATACTAT 
GTTGATCACC TCAACCAGAA AACTCAGTTT GAAAATCCAG TGGAGGAAGC GAAAAGGAAA 
AAGCAGATAG GACAGGCTGA AACTCATTCC GCAAAAACAG ATGTGGAAAG AGCACACTTT 
ACCCGGGACC CATCCCAACT TAAAGGTGTC CTTGTTCGAG CATCACTGAA AAAAAGCACA 
ATGGGCTTTG GTTTCACCAT TATAGGTGGA GATCGGCCTG ATGAATTTCT ACAAGTAAAA 
AATGTGCTCA AAGACGGTCC CGCTGCTCAG GATGGGAAAA TGGCACCAGG TGATGTTATT 
GTAGACATCA ATGGCAACTG TGTCCTTGGT CACACCCATG CAGATGTTGT CCAGATGTTT 
CAATTGGTAC CTGTCAACCA GTATGTCAAC CTTACTTTAT GCCGTGGTTA TGCACTTCCT 
GACGACAGTG AAGACCCTGT TGTGGACATT GTTGCTGCTA CCCCTGTTAT CAATGGACAA 
TCATTAGCAA AGGGAGAGGC GTGCATGAGT ACTCAGGATT TTAAACTGGG AGCAATGGTT 
TTGGATCAGA ATGGAAAATC AGGAAAACTT TTGTCCAGTG ATCATCTCAA TGGCCCATCA 
GATTCAAATG AGCAAAGAGC ATCCCTGGCT TCATCAGGCA GCTCCCAGCC TGAACTAGTG 
ACTATCCCGC TGGTTAAGGG TCCCAAAGGC TTCGGGTTTG CAATTGCCGA TAGCCCAACT 
GGGCAGAAGG TGAAAATGAT ACTGGATAGC CAGTGGTGTC AAGGCCTTCA AAAAGGGGAT 
ATCATCAAAG AAATTTACCA TCAGAATGTG CAGAATTTAA CACATCTCCA AGTCGTAGAA 
GTACTGAAAC AGTTCCCAGT AGGTGCAGAT GTCCCGTTGC TTATCTTAAG AGGAGGTCCT 
TGTTCACCAA CTAAAACTGC CAAAATGAAA ACAGATACAA AAGAAACTTC AGGAAGTTTG 
GAGACTATAA ATGAGCCTAC TCCCCAGCCT ATGCCTTTTC CACCCAGCAT AATCAGATCA 
GGATCCCCGA AATTGGATCC TTCTGAAGTC TACCTGAAAT CTAAGACTTT ATATGAAGAT 
AAACCACCCA ACACCAAAGA CCTAGATGTC TTTCTTCGGA AACAAGAATC GGGGTTTGGC 
TTCAGGGTGC TGGGAGGAGA TGGACCTGAC CAGTCTATAT ATATTGGGGC CATCATTCCC 
CTGGGAGCAG CGGAAAAGGA TGGTAGGCTC CGTGCTGCTG ATGAATTGAT GTGTATTGAT 
GGGATTCCTG TTAAAGGGAA ATCACATAAG CAAGTCTTGG ACTTAATGAC CACTGCTGCT 
CGAAATGGCC ACGTGTTACT CACTGTGAGG AGGAAGATCT TCTATGGAGA GAAACAACCC 
GAGGATGAAA GCCCTCAAGC CTTCTCACAG AGTGGATCTC CTCGCCTAAA TCGGACAGAG 
CTTCCAACCA GGTCTGCCCC ACAGGAGTCC TATGATGTTA TCTTACAGAG GAAAGAAAAT 
GAAGGGTTTG GTTTTGTCAT CCTCACCTCT AAAAGCAAGC CGCCCCCAGG AGTTATTCCT 
CATAAAATTG GCCGGGTCAT AGATGGAAGT CCAGCTGATC GTTGTGGAAG ACTGAAAGTT 
GGAGACCACA TCTCTGCGGT GAATGGGCAG TCCATTGTTG ACCTGTCCCA TGATAATATT 
GTTCAGCTCA TCAAAGATGC TGGGGTCACC GTCACGCTGA CAGTGGTTGC TGAAGAAGAG 
CATCATGGTC CACCATCAGG AACAAACTCA GCCAGGCAGA GCCCAGCACT GCAGCACAGA 
CCCATGGGAC AGGCGCAGGC CACCCACATA CCTGGGGACA GAACTGCCCT AGAAGGTGAA 
GTTGGGAAAG ATGTCTGCAG CTCTTACAGA CACTCCTGGT CTGACCACAA GCACCTTGCA 
CAGCCGGACA CCGCGGTGAT TTCCGTGGTG GGCAGTCGGC ACAGTCAGAG CCTTGGGTGT 
TACCCTGTGG AGCTGGAGAG AGGCCCTCGA GGCTTTGGGT TCAGCCTCCG AGGAGGGAAG 
GAGTACAACA TGGGGCTGTT CATCCTTCGC CTTGCTGAGG ACGGGCCTGC CATCAAAGAC 
GGCAGAATTC ACGTTGGTGA CCAGATCGTG GAAATCAATG GGGAACCTAC GCAAGGCATC 
ACACACACGC GAGCCATTGA GCTCATTCAG GCTGGCGGGA ATAAAGTCCT CCTCCTTCTG 
AGGCCAGGAA CTGGCTTGAT ACCTGATCAT GGTTTGGCTC CTTCCGGTCT GTGCTCCTTC 
GTGAAACCTG AGCAACATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759589.1
CDS	115..3495
Translation	MSKTLKKKKHWLSKVQECAVSWAGPPGDLGAEIRGGAERGEFPYLGRLRDEPGGGGGTCCVVSGKAPSPGDVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNLYLRTIPCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDNEFDTESQRKRTTSVSKMERMDSSLPEEEEDEDKEAVNGSGSMETREMHSESSDCWMKTVPSYNQTNRSMDFRNYMMRDENLEPLPKNWEMAYTDTGTIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQIGQAETHSAKTDVERAHFTRDPSQLKGVLVRASLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKMAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYALPDDSEDPVVDIVAATPVINGQSLAKGEACMSTQDFKLGAMVLDQNGKSGKLLSSDHLNGPSDSNEQRASLASSGSSQPELVTIPLVKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPCSPTKTAKMKTDTKETSGSLETINEPTPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDESPQAFSQSGSPRLNRTELPTRSAPQESYDVILQRKENEGFGFVILTSKSKPPPGVIPHKIGRVIDGSPADRCGRLKVGDHISAVNGQSIVDLSHDNIVQLIKDAGVTVTLTVVAEEEHHGPPSGTNSARQSPALQHRPMGQAQATHIPGDRTALEGEVGKDVCSSYRHSWSDHKHLAQPDTAVISVVGSRHSQSLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGLAPSGLCSFVKPEQH

Target ORF information:

RefSeq Version	XM_008761367.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761367.2

ORF Insert Sequence:

ATGTCGAAGA CGTTGAAGAA GAAGAAGCAT TGGCTCAGCA AGGTGCAGGA GTGTGCGGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTGGGC GCTGAGATCC GCGGCGGCGC GGAGCGCGGC 
GAGTTCCCTT ACCTGGGGCG GCTCCGTGAC GAGCCTGGCG GCGGCGGAGG CACCTGCTGC 
GTGGTCTCGG GCAAGGCGCC CAGTCCTGGC GATGTGCTGT TGGAGGTGAA CGGGACGCCC 
GTCAGTGGGC TCACCAACCG GGACACCCTG GCTGTCATCC GCCACTTCCG CGAGCCAATC 
CGTCTGAAGA CCGTGAAGCC AGGCAAAGTC ATAAATAAAG ATTTGCGACA TTACCTGAGT 
CTGCAGTTTC AGAAAGGATC GATTGACCAC AAACTACAGC AGGTCATCAG AGATAACCTC 
TACTTGAGAA CCATCCCATG CACTACAAGG GCTCCCAGGG ATGGGGAAGT TCCAGGGGTG 
GACTATAATT TCATTTCTGT TGAACAATTC AAGGCACTGG AAGAAAGCGG AGCATTGTTA 
GAAAGTGGAA CGTATGATGG AAATTTCTAT GGCACGCCCA AGCCTCCAGC AGAGCCTAGT 
CCTTTCCAGC CCGATCCAGT TGATCAGGTC CTCTTTGACA ATGAGTTTGA CACAGAATCC 
CAAAGAAAAC GAACTACATC TGTCAGCAAG ATGGAAAGGA TGGACAGCTC TCTCCCTGAG 
GAAGAGGAAG ATGAGGACAA AGAAGCTGTC AATGGCAGCG GAAGCATGGA AACTAGAGAG 
ATGCATTCTG AGTCGTCTGA CTGCTGGATG AAGACTGTTC CAAGTTATAA CCAAACAAAT 
CGCTCCATGG ACTTTAGAAA TTATATGATG AGAGACGAGA ATCTGGAGCC GCTGCCCAAA 
AACTGGGAAA TGGCCTACAC TGACACAGGA ACGATCTACT TCATTGATCA CAATACGAAG 
ACAACCACCT GGCTGGATCC TCGTCTCTGC AAGAAAGCCA AAGCCCCTGA AGACTGTGAG 
GATGGAGAGC TTCCATATGG CTGGGAGAAA ATAGAGGACC CTCAGTATGG AACATACTAT 
GTTGATCACC TCAACCAGAA AACTCAGTTT GAAAATCCAG TGGAGGAAGC GAAAAGGAAA 
AAGCAGATAG GACAGGCTGA AACTCATTCC GCAAAAACAG ATGTGGAAAG AGCACACTTT 
ACCCGGGACC CATCCCAACT TAAAGGTGTC CTTGTTCGAG CATCACTGAA AAAAAGCACA 
ATGGGCTTTG GTTTCACCAT TATAGGTGGA GATCGGCCTG ATGAATTTCT ACAAGTAAAA 
AATGTGCTCA AAGACGGTCC CGCTGCTCAG GATGGGAAAA TGGCACCAGG TGATGTTATT 
GTAGACATCA ATGGCAACTG TGTCCTTGGT CACACCCATG CAGATGTTGT CCAGATGTTT 
CAATTGGTAC CTGTCAACCA GTATGTCAAC CTTACTTTAT GCCGTGGTTA TGCACTTCCT 
GACGACAGTG AAGACCCTGT TGTGGACATT GTTGCTGCTA CCCCTGTTAT CAATGGACAA 
TCATTAGCAA AGGGAGAGGC GTGCATGAGT ACTCAGGATT TTAAACTGGG AGCAATGGTT 
TTGGATCAGA ATGGAAAATC AGGAAAACTT TTGTCCAGTG ATCATCTCAA TGGCCCATCA 
GATTCAAATG AGCAAAGAGC ATCCCTGGCT TCATCAGGCA GCTCCCAGCC TGAACTAGTG 
ACTATCCCGC TGGTTAAGGG TCCCAAAGGC TTCGGGTTTG CAATTGCCGA TAGCCCAACT 
GGGCAGAAGG TGAAAATGAT ACTGGATAGC CAGTGGTGTC AAGGCCTTCA AAAAGGGGAT 
ATCATCAAAG AAATTTACCA TCAGAATGTG CAGAATTTAA CACATCTCCA AGTCGTAGAA 
GTACTGAAAC AGTTCCCAGT AGGTGCAGAT GTCCCGTTGC TTATCTTAAG AGGAGGTCCT 
TGTTCACCAA CTAAAACTGC CAAAATGAAA ACAGATACAA AAGAAACTTC AGGAAGTTTG 
GAGACTATAA ATGAGCCTAC TCCCCAGCCT ATGCCTTTTC CACCCAGCAT AATCAGATCA 
GGATCCCCGA AATTGGATCC TTCTGAAGTC TACCTGAAAT CTAAGACTTT ATATGAAGAT 
AAACCACCCA ACACCAAAGA CCTAGATGTC TTTCTTCGGA AACAAGAATC GGGGTTTGGC 
TTCAGGGTGC TGGGAGGAGA TGGACCTGAC CAGTCTATAT ATATTGGGGC CATCATTCCC 
CTGGGAGCAG CGGAAAAGGA TGGTAGGCTC CGTGCTGCTG ATGAATTGAT GTGTATTGAT 
GGGATTCCTG TTAAAGGGAA ATCACATAAG CAAGTCTTGG ACTTAATGAC CACTGCTGCT 
CGAAATGGCC ACGTGTTACT CACTGTGAGG AGGAAGATCT TCTATGGAGA GAAACAACCC 
GAGGATGAAA GCCCTCAAGC CTTCTCACAG AGTGGATCTC CTCGCCTAAA TCGGACAGAG 
CTTCCAACCA GGTCTGCCCC ACAGGAGTCC TATGATGTTA TCTTACAGAG GAAAGAAAAT 
GAAGGGTTTG GTTTTGTCAT CCTCACCTCT AAAAGCAAGC CGCCCCCAGG AGTTATTCCT 
CATAAAATTG GCCGGGTCAT AGATGGAAGT CCAGCTGATC GTTGTGGAAG ACTGAAAGTT 
GGAGACCACA TCTCTGCGGT GAATGGGCAG TCCATTGTTG ACCTGTCCCA TGATAATATT 
GTTCAGCTCA TCAAAGATGC TGGGGTCACC GTCACGCTGA CAGTGGTTGC TGAAGAAGAG 
CATCATGGTC CACCATCAGG AACAAACTCA GCCAGGCAGA GCCCAGCACT GCAGCACAGA 
CCCATGGGAC AGGCGCAGGC CACCCACATA CCTGGGGACA GAACTGCCCT AGAAGGTGAA 
GTTGGGAAAG ATGTCTGCAG CTCTTACAGA CACTCCTGGT CTGACCACAA GCACCTTGCA 
CAGCCGGACA CCGCGGTGAT TTCCGTGGTG GGCAGTCGGC ACAGTCAGAG CCTTGGGTGT 
TACCCTGTGG AGCTGGAGAG AGGCCCTCGA GGCTTTGGGT TCAGCCTCCG AGGAGGGAAG 
GAGTACAACA TGGGGCTGTT CATCCTTCGC CTTGCTGAGG ACGGGCCTGC CATCAAAGAC 
GGCAGAATTC ACGTTGGTGA CCAGATCGTG GAAATCAATG GGGAACCTAC GCAAGGCATC 
ACACACACGC GAGCCATTGA GCTCATTCAG GCTGGCGGGA ATAAAGTCCT CCTCCTTCTG 
AGGCCAGGAA CTGGCTTGAT ACCTGATCAT GGTTTGGCTC CTTCCGGTCT GTGCTCCTTC 
GTGAAACCTG AGCAACATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteomic analysis of beta1-adrenergic receptor interactions with PDZ scaffold proteins.
The Journal of biological chemistry281(5)2820-7(2006 Feb)
He J,Bellini M,Inuzuka H,Xu J,Xiong Y,Yang X,Castleberry AM,Hall RA

Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases.
The Journal of biological chemistry280(32)28936-43(2005 Aug)
Valiente M,Andrés-Pons A,Gomar B,Torres J,Gil A,Tapparel C,Antonarakis SE,Pulido R

Combined genealogical, mapping, and expression approaches to identify spontaneously hypertensive rat hypertension candidate genes.
Hypertension (Dallas, Tex. : 1979)45(4)698-704(2005 Apr)
Hinojos CA,Boerwinkle E,Fornage M,Doris PA

The complexity of PDZ domain-mediated interactions at glutamatergic synapses: a case study on neuroligin.
Neuropharmacology47(5)724-33(2004 Oct)
Meyer G,Varoqueaux F,Neeb A,Oschlies M,Brose N

MAGI-3 is involved in the regulation of the JNK signaling pathway as a scaffold protein for frizzled and Ltap.
Oncogene23(36)6023-30(2004 Aug)
Yao R,Natsume Y,Noda T

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Magi3 cDNA ORF clone, Rattus norvegicus(Norway rat)

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