Mpg cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mpg
Entrez Gene ID	24561
Full Name	N-methylpurine-DNA glycosylase
General protein information	Preferred Names N-methylpurine-DNA glycosylase Names DNA-3-methyladenine glycosylase 3-alkyladenine DNA glycosylase 3-methyladenine DNA glycosidase ADPG N-methylpurine-DNA glycocylase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12
Summary	enzyme that cleaves 3-methyladenine and 7-methylguanine residues from DNA [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012601.2	NP_036733.2	DNA-3-methyladenine glycosylase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MPG	XP_005621833.1
Rattus norvegicus (Norway rat)	Mpg	NP_036733.2
Gallus gallus (chicken)	MPG	XP_414945.3
Danio rerio (zebrafish)	mpg	NP_001076319.1
Homo sapiens (human)	MPG	NP_001015052.1
Macaca mulatta (Rhesus monkey)	MPG	XP_001118427.2
Bos taurus (cattle)	MPG	XP_002683855.2
Mus musculus (house mouse)	Mpg	NP_034952.2
Xenopus tropicalis (tropical clawed frog)	LOC549932	NP_001017178.1

Gene Ontology
Bibliography

Related articles in PubMed

High-density rat radiation hybrid maps containing over 24,000 SSLPs, genes, and ESTs provide a direct link to the rat genome sequence.
Kwitek AE, Gullings-Handley J, Yu J, Carlos DC, Orlebeke K, Nie J, Eckert J, Lemke A, Andrae JW, Bromberg S, Pasko D, Chen D, Scheetz TE, Casavant TL, Soares MB, Sheffield VC, Tonellato PJ, Jacob HJ
Genome research14(4)750-7(2004 Apr)

New immunoaffinity-LC-MS/MS methodology reveals that Aag null mice are deficient in their ability to clear 1,N6-etheno-deoxyadenosine DNA lesions from lung and liver in vivo.
Ham AJ, Engelward BP, Koc H, Sangaiah R, Meira LB, Samson LD, Swenberg JA
DNA repair3(3)257-65(2004 Mar)

Targeted deletion of alkylpurine-DNA-N-glycosylase in mice eliminates repair of 1,N6-ethenoadenine and hypoxanthine but not of 3,N4-ethenocytosine or 8-oxoguanine.
Hang B, Singer B, Margison GP, Elder RH
Proceedings of the National Academy of Sciences of the United States of America94(24)12869-74(1997 Nov)

Cloning and characterization of a mouse 3-methyladenine/7-methyl-guanine/3-methylguanine DNA glycosylase cDNA whose gene maps to chromosome 11.
Engelward BP, Boosalis MS, Chen BJ, Deng Z, Siciliano MJ, Samson LD
Carcinogenesis14(2)175-81(1993 Feb)

Isolation and structure of a cDNA expressing a mammalian 3-methyladenine-DNA glycosylase.
O'Connor TR, Laval F
The EMBO journal9(10)3337-42(1990 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mpg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mpg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00428	NM_012601.2 Latest version!	Rattus norvegicus N-methylpurine-DNA glycosylase (Mpg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mpg ( NM_012601.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036733.2 Rattus norvegicus N-methylpurine-DNA glycosylase (Mpg), mRNA.

Price & Availability↑

CloneID	ORa00428
Clone ID Related Accession (Same CDS sequence)	NM_012601.2
Accession Version	NM_012601.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Rattus norvegicus(Norway rat)
Product	DNA-3-methyladenine glycosylase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC096051.7, AW435341.1, CB765983.1 and AA899760.1. On Oct 17, 2007 this sequence version replaced NM_012601.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## Transcript exon combination :: X56420.1, FN804928.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCGGCC GCGGTGGTAC TGCGCGCCTG GGCAGAGGAT CCCTAAAACC GGTGTCCGTG 
GTGCTCCCCG ACACCGAGCA CCCTGCATTT CCTGGACGAA CCCGCCGCCC AGGGAATGCC 
AGAGCAGGAT CCCAAGTGAC CGGATCTCGT GAGGTCGGCC AGATGCCAGC GCCGCTTTCA 
CGAAAGATCG GGCAAAAAAA GCAGCAACTG GCACAGTCAG AGCAGCAGCA GACCCCTAAG 
GAGAGACTCT CTTCGACCCC TGGCCTCCTG CGAAGTATCT ACTTCTCGAG CCCAGAGGAC 
CGCCCTGCCC GGCTAGGACC GGAGTATTTT GACCAGCCCG CAGTCACCCT AGCCCGTGCA 
TTTCTGGGAC AGGTCCTTGT CCGGCGACTT GCTGATGGAA CAGAACTCCG TGGGCGCATT 
GTAGAGACTG AGGCATACTT GGGGCCAGAA GACGAAGCTG CCCACTCAAG GGGTGGCCGG 
CAGACCCCCC GTAACCGTGG CATGTTCATG AAACCTGGAA CTCTGTACGT GTACCTCATC 
TATGGCATGT ACTTCTGCTT GAATGTCTCC AGTCAAGGGG CTGGGGCTTG TGTCTTGCTA 
AGAGCACTAG AGCCATTGGA GGGCCTAGAG ACCATGCGGC AGCTTCGAAA CTCCCTCCGG 
AAAAGCACTG TCGGCCGTTC CCTCAAGGAC CGCGAGCTCT GTAATGGTCC CTCCAAACTG 
TGCCAGGCCC TAGCCATTGA TAAGAGCTTT GACCAGCGAG ACCTGGCCCA AGATGAGGCT 
GTGTGGCTGG AGCATGGCCC TCTGGAGTCC AGCAGCCCAG CTGTGGTGGC GGCAGCCCGC 
ATAGGTATTG GCCATGCAGG GGAATGGACA CAGAAGCCCT TGCGCTTCTA TGTCCAGGGC 
AGCCCATGGG TCAGTGTGGT AGATAGAGTG GCTGAACAGA TGTATCAGCC TCAGCAAACA 
GCGTGCTCTG ATTGTTCAAA AGTAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036733.2
CDS	1..990
Translation	MRGRGGTARLGRGSLKPVSVVLPDTEHPAFPGRTRRPGNARAGSQVTGSREVGQMPAPLSRKIGQKKQQLAQSEQQQTPKERLSSTPGLLRSIYFSSPEDRPARLGPEYFDQPAVTLARAFLGQVLVRRLADGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYLIYGMYFCLNVSSQGAGACVLLRALEPLEGLETMRQLRNSLRKSTVGRSLKDRELCNGPSKLCQALAIDKSFDQRDLAQDEAVWLEHGPLESSSPAVVAAARIGIGHAGEWTQKPLRFYVQGSPWVSVVDRVAEQMYQPQQTACSDCSKVK

Target ORF information:

RefSeq Version	NM_012601.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N-methylpurine-DNA glycosylase (Mpg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012601.2

ORF Insert Sequence:

ATGCGCGGCC GCGGTGGTAC TGCGCGCCTG GGCAGAGGAT CCCTAAAACC GGTGTCCGTG 
GTGCTCCCCG ACACCGAGCA CCCTGCATTT CCTGGACGAA CCCGCCGCCC AGGGAATGCC 
AGAGCAGGAT CCCAAGTGAC CGGATCTCGT GAGGTCGGCC AGATGCCAGC GCCGCTTTCA 
CGAAAGATCG GGCAAAAAAA GCAGCAACTG GCACAGTCAG AGCAGCAGCA GACCCCTAAG 
GAGAGACTCT CTTCGACCCC TGGCCTCCTG CGAAGTATCT ACTTCTCGAG CCCAGAGGAC 
CGCCCTGCCC GGCTAGGACC GGAGTATTTT GACCAGCCCG CAGTCACCCT AGCCCGTGCA 
TTTCTGGGAC AGGTCCTTGT CCGGCGACTT GCTGATGGAA CAGAACTCCG TGGGCGCATT 
GTAGAGACTG AGGCATACTT GGGGCCAGAA GACGAAGCTG CCCACTCAAG GGGTGGCCGG 
CAGACCCCCC GTAACCGTGG CATGTTCATG AAACCTGGAA CTCTGTACGT GTACCTCATC 
TATGGCATGT ACTTCTGCTT GAATGTCTCC AGTCAAGGGG CTGGGGCTTG TGTCTTGCTA 
AGAGCACTAG AGCCATTGGA GGGCCTAGAG ACCATGCGGC AGCTTCGAAA CTCCCTCCGG 
AAAAGCACTG TCGGCCGTTC CCTCAAGGAC CGCGAGCTCT GTAATGGTCC CTCCAAACTG 
TGCCAGGCCC TAGCCATTGA TAAGAGCTTT GACCAGCGAG ACCTGGCCCA AGATGAGGCT 
GTGTGGCTGG AGCATGGCCC TCTGGAGTCC AGCAGCCCAG CTGTGGTGGC GGCAGCCCGC 
ATAGGTATTG GCCATGCAGG GGAATGGACA CAGAAGCCCT TGCGCTTCTA TGTCCAGGGC 
AGCCCATGGG TCAGTGTGGT AGATAGAGTG GCTGAACAGA TGTATCAGCC TCAGCAAACA 
GCGTGCTCTG ATTGTTCAAA AGTAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-density rat radiation hybrid maps containing over 24,000 SSLPs, genes, and ESTs provide a direct link to the rat genome sequence.
Genome research14(4)750-7(2004 Apr)
Kwitek AE,Gullings-Handley J,Yu J,Carlos DC,Orlebeke K,Nie J,Eckert J,Lemke A,Andrae JW,Bromberg S,Pasko D,Chen D,Scheetz TE,Casavant TL,Soares MB,Sheffield VC,Tonellato PJ,Jacob HJ

New immunoaffinity-LC-MS/MS methodology reveals that Aag null mice are deficient in their ability to clear 1,N6-etheno-deoxyadenosine DNA lesions from lung and liver in vivo.
DNA repair3(3)257-65(2004 Mar)
Ham AJ,Engelward BP,Koc H,Sangaiah R,Meira LB,Samson LD,Swenberg JA

Targeted deletion of alkylpurine-DNA-N-glycosylase in mice eliminates repair of 1,N6-ethenoadenine and hypoxanthine but not of 3,N4-ethenocytosine or 8-oxoguanine.
Proceedings of the National Academy of Sciences of the United States of America94(24)12869-74(1997 Nov)
Hang B,Singer B,Margison GP,Elder RH

Cloning and characterization of a mouse 3-methyladenine/7-methyl-guanine/3-methylguanine DNA glycosylase cDNA whose gene maps to chromosome 11.
Carcinogenesis14(2)175-81(1993 Feb)
Engelward BP,Boosalis MS,Chen BJ,Deng Z,Siciliano MJ,Samson LD

Isolation and structure of a cDNA expressing a mammalian 3-methyladenine-DNA glycosylase.
The EMBO journal9(10)3337-42(1990 Oct)
O'Connor TR,Laval F