Grm1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grm1
Entrez Gene ID	24414
Full Name	glutamate receptor, metabotropic 1
Synonyms	Gprc1a
General protein information	Preferred Names glutamate receptor, metabotropic 1 Names metabotropic glutamate receptor 1 G protein coupled receptor family C group 1 member A mGluR1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1p13
Summary	may play a role in synaptic transmission; may modulate perception of pain in arthritis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001114330.1	NP_001107802.1	metabotropic glutamate receptor 1 isoform beta precursor
NM_017011.1	NP_058707.1	metabotropic glutamate receptor 1 isoform alpha precursor
XM_017588777.1	XP_017444266.1	metabotropic glutamate receptor 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GRM1	NP_001158173.1
Macaca mulatta (Rhesus monkey)	GRM1	XP_001085942.1
Bos taurus (cattle)	GRM1	NP_001192933.1
Mus musculus (house mouse)	Grm1	NP_058672.1
Rattus norvegicus (Norway rat)	Grm1	NP_058707.1
Danio rerio (zebrafish)	grm1	XP_001921594.3
Canis lupus familiaris (dog)	GRM1	XP_005615547.1
Xenopus tropicalis (tropical clawed frog)	grm1	XP_002931565.2
Homo sapiens (human)	GRM1	NP_001264993.1
Gallus gallus (chicken)	GRM1	XP_004935642.1

Gene Ontology
Bibliography

Related articles in PubMed

Transient Switching of NMDA-Dependent Long-Term Synaptic Potentiation in CA3-CA1 Hippocampal Synapses to mGluR
Postnikova TY, Trofimova AM, Ergina JL, Zubareva OE, Kalemenev SV, Zaitsev AV
Cellular and molecular neurobiology39(2)287-300(2019 Mar)

Amphetamine-induced Conditioned Place Preference and Changes in mGlu1/5 Receptor Expression and Signaling in the Rat Medial Prefrontal Cortex.
Mao LM, Wang JQ
Neuroscience400110-119(2019 02)

The Probability of Neurotransmitter Release Governs AMPA Receptor Trafficking via Activity-Dependent Regulation of mGluR1 Surface Expression.
Sanderson TM, Bradley CA, Georgiou J, Hong YH, Ng AN, Lee Y, Kim HD, Kim D, Amici M, Son GH, Zhuo M, Kim K, Kaang BK, Kim SJ, Collingridge GL
Cell reports25(13)3631-3646.e3(2018 12)

In Vivo Monitoring for Regional Changes of Metabotropic Glutamate Receptor Subtype 1 (mGluR1) in Pilocarpine-Induced Epileptic Rat Brain by Small-Animal PET.
Yamasaki T, Fujinaga M, Mori W, Zhang Y, Wakizaka H, Nengaki N, Xie L, Hatori A, Zhang MR
Scientific reports7(1)14945(2017 11)

Effects of GABACR and mGluR1 antagonists on contrast response functions of Sprague-Dawley and P23H rat retinal ganglion cells.
Jensen R
PloS one12(12)e0189980(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Grm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13360	NM_001114330.1 Latest version!	Rattus norvegicus glutamate metabotropic receptor 1 (Grm1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa13373	NM_017011.1 Latest version!	Rattus norvegicus glutamate metabotropic receptor 1 (Grm1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa56381	XM_017588777.1 Latest version!	Rattus norvegicus glutamate receptor, metabotropic 1 (Grm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grm1 ( NM_001114330.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001107802.1 Rattus norvegicus glutamate metabotropic receptor 1 (Grm1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa13360
Clone ID Related Accession (Same CDS sequence)	NM_001114330.1
Accession Version	NM_001114330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575820800000
Organism	Rattus norvegicus(Norway rat)
Product	metabotropic glutamate receptor 1 isoform beta precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M61099.1 and Y18810.1. Transcript Variant: his variant (2) encodes the shorter isoform (beta). Isoform beta has a different subcellular distribution than isoform alpha and may be functionally distinct due to differential G-protein coupling of its novel C-terminal sequence. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTCCGGC TCCTCTTGAT TTTCTTCCCA ATGATCTTTT TGGAGATGTC CATTTTGCCC 
AGGATGCCTG ACAGAAAAGT ATTGCTGGCA GGTGCCTCGT CCCAGCGCTC CGTGGCGAGA 
ATGGACGGAG ATGTCATCAT CGGAGCCCTC TTCTCAGTCC ATCACCAGCC TCCAGCCGAG 
AAGGTACCCG AAAGGAAGTG TGGGGAGATC AGGGAACAGT ATGGTATCCA GAGGGTGGAG 
GCCATGTTCC ACACGTTGGA TAAGATTAAC GCGGACCCGG TGCTCCTGCC CAACATCACT 
CTGGGCAGTG AGATCCGGGA CTCCTGCTGG CACTCTTCAG TGGCTCTCGA ACAGAGCATC 
GAATTCATCA GAGACTCCCT GATTTCCATC CGAGATGAGA AGGATGGGCT GAACCGATGC 
CTGCCTGATG GCCAGACCCT GCCCCCTGGC AGGACTAAGA AGCCTATTGC TGGAGTGATC 
GGCCCTGGCT CCAGCTCTGT GGCCATTCAA GTCCAGAATC TTCTCCAGCT GTTCGACATC 
CCACAGATCG CCTATTCTGC CACAAGCATA GACCTGAGTG ACAAAACTTT GTACAAATAC 
TTCCTGAGGG TGGTCCCTTC TGACACTTTG CAGGCAAGGG CGATGCTCGA CATAGTCAAG 
CGTTACAACT GGACCTATGT CTCAGCAGTC CACACAGAAG GGAATTACGG CGAGAGTGGA 
ATGGATGCTT TCAAAGAACT GGCTGCCCAG GAAGGCCTCT GCATCGCACA CTCGGACAAA 
ATCTACAGCA ATGCTGGCGA GAAGAGCTTT GACCGGCTCC TGCGTAAACT CCGGGAGCGG 
CTTCCCAAGG CCAGGGTTGT GGTCTGCTTC TGCGAGGGCA TGACAGTGCG GGGCTTACTG 
AGTGCCATGC GCCGCCTGGG CGTCGTGGGC GAGTTCTCAC TCATTGGAAG TGATGGATGG 
GCAGACAGAG ATGAAGTCAT CGAAGGCTAT GAGGTGGAAG CCAACGGAGG GATCACAATA 
AAGCTTCAGT CTCCAGAGGT CAGGTCATTT GATGACTACT TCCTGAAGCT GAGGCTGGAC 
ACCAACACAA GGAATCCTTG GTTCCCTGAG TTCTGGCAAC ATCGCTTCCA GTGTCGCCTA 
CCTGGACACC TCTTGGAAAA CCCCAACTTT AAGAAAGTGT GCACAGGAAA TGAAAGCTTG 
GAAGAAAACT ATGTCCAGGA CAGCAAAATG GGATTTGTCA TCAATGCCAT CTATGCCATG 
GCACATGGGC TGCAGAACAT GCACCATGCT CTGTGTCCCG GCCATGTGGG CCTGTGTGAT 
GCTATGAAAC CCATTGATGG CAGGAAGCTC CTGGATTTCC TCATCAAATC CTCTTTTGTC 
GGAGTGTCTG GAGAGGAGGT GTGGTTCGAT GAGAAGGGGG ATGCTCCCGG AAGGTATGAC 
ATTATGAATC TGCAGTACAC AGAAGCTAAT CGCTATGACT ATGTCCACGT GGGGACCTGG 
CATGAAGGAG TGCTGAATAT TGATGATTAC AAAATCCAGA TGAACAAAAG CGGAATGGTA 
CGATCTGTGT GCAGTGAGCC TTGCTTAAAG GGTCAGATTA AGGTCATACG GAAAGGAGAA 
GTGAGCTGCT GCTGGATCTG CACGGCCTGC AAAGAGAATG AGTTTGTGCA GGACGAGTTC 
ACCTGCAGAG CCTGTGACCT GGGGTGGTGG CCCAACGCAG AGCTCACAGG CTGTGAGCCC 
ATTCCTGTCC GTTATCTTGA GTGGAGTGAC ATAGAATCTA TCATAGCCAT CGCCTTTTCT 
TGCCTGGGCA TCCTCGTGAC GCTGTTTGTC ACCCTCATCT TCGTTCTGTA CCGGGACACA 
CCCGTGGTCA AATCCTCCAG TAGGGAGCTC TGCTATATCA TTCTGGCTGG TATTTTCCTC 
GGCTATGTGT GCCCTTTCAC CCTCATCGCC AAACCTACTA CCACATCCTG CTACCTCCAG 
CGCCTCCTAG TTGGCCTCTC TTCTGCCATG TGCTACTCTG CTTTAGTGAC CAAAACCAAT 
CGTATTGCAC GCATCCTGGC TGGCAGCAAG AAGAAGATCT GCACCCGGAA GCCCAGATTC 
ATGAGCGCTT GGGCCCAAGT GATCATAGCC TCCATTCTGA TTAGTGTACA GCTAACACTA 
GTGGTGACCT TGATCATCAT GGAGCCTCCC ATGCCCATTT TGTCCTACCC GAGTATCAAG 
GAAGTCTACC TTATCTGCAA TACCAGCAAC CTGGGTGTAG TGGCCCCTGT GGGTTACAAT 
GGACTCCTCA TCATGAGCTG TACCTACTAT GCCTTCAAGA CCCGCAACGT GCCGGCCAAC 
TTCAATGAGG CTAAATACAT CGCCTTCACC ATGTACACTA CCTGCATCAT CTGGCTGGCT 
TTCGTTCCCA TTTACTTTGG GAGCAACTAC AAGATCATCA CTACCTGCTT CGCGGTGAGC 
CTCAGTGTGA CGGTGGCCCT GGGGTGCATG TTTACTCCGA AGATGTACAT CATCATTGCC 
AAACCTGAGA GGAACGTCCG CAGTGCCTTC ACGACCTCTG ATGTTGTCCG CATGCACGTC 
GGTGATGGCA AACTGCCGTG CCGCTCCAAC ACCTTCCTCA ACATTTTCCG GAGAAAGAAG 
CCCGGGGCAG GGAATGCCAA GAAGAGGCAG CCAGAATTCT CGCCCAGCAG CCAGTGTCCG 
TCGGCACATG CGCAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107802.1
CDS	377..3097
Translation	MVRLLLIFFPMIFLEMSILPRMPDRKVLLAGASSQRSVARMDGDVIIGALFSVHHQPPAEKVPERKCGEIREQYGIQRVEAMFHTLDKINADPVLLPNITLGSEIRDSCWHSSVALEQSIEFIRDSLISIRDEKDGLNRCLPDGQTLPPGRTKKPIAGVIGPGSSSVAIQVQNLLQLFDIPQIAYSATSIDLSDKTLYKYFLRVVPSDTLQARAMLDIVKRYNWTYVSAVHTEGNYGESGMDAFKELAAQEGLCIAHSDKIYSNAGEKSFDRLLRKLRERLPKARVVVCFCEGMTVRGLLSAMRRLGVVGEFSLIGSDGWADRDEVIEGYEVEANGGITIKLQSPEVRSFDDYFLKLRLDTNTRNPWFPEFWQHRFQCRLPGHLLENPNFKKVCTGNESLEENYVQDSKMGFVINAIYAMAHGLQNMHHALCPGHVGLCDAMKPIDGRKLLDFLIKSSFVGVSGEEVWFDEKGDAPGRYDIMNLQYTEANRYDYVHVGTWHEGVLNIDDYKIQMNKSGMVRSVCSEPCLKGQIKVIRKGEVSCCWICTACKENEFVQDEFTCRACDLGWWPNAELTGCEPIPVRYLEWSDIESIIAIAFSCLGILVTLFVTLIFVLYRDTPVVKSSSRELCYIILAGIFLGYVCPFTLIAKPTTTSCYLQRLLVGLSSAMCYSALVTKTNRIARILAGSKKKICTRKPRFMSAWAQVIIASILISVQLTLVVTLIIMEPPMPILSYPSIKEVYLICNTSNLGVVAPVGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTCFAVSLSVTVALGCMFTPKMYIIIAKPERNVRSAFTTSDVVRMHVGDGKLPCRSNTFLNIFRRKKPGAGNAKKRQPEFSPSSQCPSAHAQL

Target ORF information:

RefSeq Version	NM_001114330.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate metabotropic receptor 1 (Grm1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114330.1

ORF Insert Sequence:

ATGGTCCGGC TCCTCTTGAT TTTCTTCCCA ATGATCTTTT TGGAGATGTC CATTTTGCCC 
AGGATGCCTG ACAGAAAAGT ATTGCTGGCA GGTGCCTCGT CCCAGCGCTC CGTGGCGAGA 
ATGGACGGAG ATGTCATCAT CGGAGCCCTC TTCTCAGTCC ATCACCAGCC TCCAGCCGAG 
AAGGTACCCG AAAGGAAGTG TGGGGAGATC AGGGAACAGT ATGGTATCCA GAGGGTGGAG 
GCCATGTTCC ACACGTTGGA TAAGATTAAC GCGGACCCGG TGCTCCTGCC CAACATCACT 
CTGGGCAGTG AGATCCGGGA CTCCTGCTGG CACTCTTCAG TGGCTCTCGA ACAGAGCATC 
GAATTCATCA GAGACTCCCT GATTTCCATC CGAGATGAGA AGGATGGGCT GAACCGATGC 
CTGCCTGATG GCCAGACCCT GCCCCCTGGC AGGACTAAGA AGCCTATTGC TGGAGTGATC 
GGCCCTGGCT CCAGCTCTGT GGCCATTCAA GTCCAGAATC TTCTCCAGCT GTTCGACATC 
CCACAGATCG CCTATTCTGC CACAAGCATA GACCTGAGTG ACAAAACTTT GTACAAATAC 
TTCCTGAGGG TGGTCCCTTC TGACACTTTG CAGGCAAGGG CGATGCTCGA CATAGTCAAG 
CGTTACAACT GGACCTATGT CTCAGCAGTC CACACAGAAG GGAATTACGG CGAGAGTGGA 
ATGGATGCTT TCAAAGAACT GGCTGCCCAG GAAGGCCTCT GCATCGCACA CTCGGACAAA 
ATCTACAGCA ATGCTGGCGA GAAGAGCTTT GACCGGCTCC TGCGTAAACT CCGGGAGCGG 
CTTCCCAAGG CCAGGGTTGT GGTCTGCTTC TGCGAGGGCA TGACAGTGCG GGGCTTACTG 
AGTGCCATGC GCCGCCTGGG CGTCGTGGGC GAGTTCTCAC TCATTGGAAG TGATGGATGG 
GCAGACAGAG ATGAAGTCAT CGAAGGCTAT GAGGTGGAAG CCAACGGAGG GATCACAATA 
AAGCTTCAGT CTCCAGAGGT CAGGTCATTT GATGACTACT TCCTGAAGCT GAGGCTGGAC 
ACCAACACAA GGAATCCTTG GTTCCCTGAG TTCTGGCAAC ATCGCTTCCA GTGTCGCCTA 
CCTGGACACC TCTTGGAAAA CCCCAACTTT AAGAAAGTGT GCACAGGAAA TGAAAGCTTG 
GAAGAAAACT ATGTCCAGGA CAGCAAAATG GGATTTGTCA TCAATGCCAT CTATGCCATG 
GCACATGGGC TGCAGAACAT GCACCATGCT CTGTGTCCCG GCCATGTGGG CCTGTGTGAT 
GCTATGAAAC CCATTGATGG CAGGAAGCTC CTGGATTTCC TCATCAAATC CTCTTTTGTC 
GGAGTGTCTG GAGAGGAGGT GTGGTTCGAT GAGAAGGGGG ATGCTCCCGG AAGGTATGAC 
ATTATGAATC TGCAGTACAC AGAAGCTAAT CGCTATGACT ATGTCCACGT GGGGACCTGG 
CATGAAGGAG TGCTGAATAT TGATGATTAC AAAATCCAGA TGAACAAAAG CGGAATGGTA 
CGATCTGTGT GCAGTGAGCC TTGCTTAAAG GGTCAGATTA AGGTCATACG GAAAGGAGAA 
GTGAGCTGCT GCTGGATCTG CACGGCCTGC AAAGAGAATG AGTTTGTGCA GGACGAGTTC 
ACCTGCAGAG CCTGTGACCT GGGGTGGTGG CCCAACGCAG AGCTCACAGG CTGTGAGCCC 
ATTCCTGTCC GTTATCTTGA GTGGAGTGAC ATAGAATCTA TCATAGCCAT CGCCTTTTCT 
TGCCTGGGCA TCCTCGTGAC GCTGTTTGTC ACCCTCATCT TCGTTCTGTA CCGGGACACA 
CCCGTGGTCA AATCCTCCAG TAGGGAGCTC TGCTATATCA TTCTGGCTGG TATTTTCCTC 
GGCTATGTGT GCCCTTTCAC CCTCATCGCC AAACCTACTA CCACATCCTG CTACCTCCAG 
CGCCTCCTAG TTGGCCTCTC TTCTGCCATG TGCTACTCTG CTTTAGTGAC CAAAACCAAT 
CGTATTGCAC GCATCCTGGC TGGCAGCAAG AAGAAGATCT GCACCCGGAA GCCCAGATTC 
ATGAGCGCTT GGGCCCAAGT GATCATAGCC TCCATTCTGA TTAGTGTACA GCTAACACTA 
GTGGTGACCT TGATCATCAT GGAGCCTCCC ATGCCCATTT TGTCCTACCC GAGTATCAAG 
GAAGTCTACC TTATCTGCAA TACCAGCAAC CTGGGTGTAG TGGCCCCTGT GGGTTACAAT 
GGACTCCTCA TCATGAGCTG TACCTACTAT GCCTTCAAGA CCCGCAACGT GCCGGCCAAC 
TTCAATGAGG CTAAATACAT CGCCTTCACC ATGTACACTA CCTGCATCAT CTGGCTGGCT 
TTCGTTCCCA TTTACTTTGG GAGCAACTAC AAGATCATCA CTACCTGCTT CGCGGTGAGC 
CTCAGTGTGA CGGTGGCCCT GGGGTGCATG TTTACTCCGA AGATGTACAT CATCATTGCC 
AAACCTGAGA GGAACGTCCG CAGTGCCTTC ACGACCTCTG ATGTTGTCCG CATGCACGTC 
GGTGATGGCA AACTGCCGTG CCGCTCCAAC ACCTTCCTCA ACATTTTCCG GAGAAAGAAG 
CCCGGGGCAG GGAATGCCAA GAAGAGGCAG CCAGAATTCT CGCCCAGCAG CCAGTGTCCG 
TCGGCACATG CGCAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grm1 ( NM_017011.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058707.1 Rattus norvegicus glutamate metabotropic receptor 1 (Grm1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa13373
Clone ID Related Accession (Same CDS sequence)	NM_017011.1
Accession Version	NM_017011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575820800000
Organism	Rattus norvegicus(Norway rat)
Product	metabotropic glutamate receptor 1 isoform alpha precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M61099.1. Transcript Variant: This variant (1) encodes the longer isoform (alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M61099.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTCCGGC TCCTCTTGAT TTTCTTCCCA ATGATCTTTT TGGAGATGTC CATTTTGCCC 
AGGATGCCTG ACAGAAAAGT ATTGCTGGCA GGTGCCTCGT CCCAGCGCTC CGTGGCGAGA 
ATGGACGGAG ATGTCATCAT CGGAGCCCTC TTCTCAGTCC ATCACCAGCC TCCAGCCGAG 
AAGGTACCCG AAAGGAAGTG TGGGGAGATC AGGGAACAGT ATGGTATCCA GAGGGTGGAG 
GCCATGTTCC ACACGTTGGA TAAGATTAAC GCGGACCCGG TGCTCCTGCC CAACATCACT 
CTGGGCAGTG AGATCCGGGA CTCCTGCTGG CACTCTTCAG TGGCTCTCGA ACAGAGCATC 
GAATTCATCA GAGACTCCCT GATTTCCATC CGAGATGAGA AGGATGGGCT GAACCGATGC 
CTGCCTGATG GCCAGACCCT GCCCCCTGGC AGGACTAAGA AGCCTATTGC TGGAGTGATC 
GGCCCTGGCT CCAGCTCTGT GGCCATTCAA GTCCAGAATC TTCTCCAGCT GTTCGACATC 
CCACAGATCG CCTATTCTGC CACAAGCATA GACCTGAGTG ACAAAACTTT GTACAAATAC 
TTCCTGAGGG TGGTCCCTTC TGACACTTTG CAGGCAAGGG CGATGCTCGA CATAGTCAAG 
CGTTACAACT GGACCTATGT CTCAGCAGTC CACACAGAAG GGAATTACGG CGAGAGTGGA 
ATGGATGCTT TCAAAGAACT GGCTGCCCAG GAAGGCCTCT GCATCGCACA CTCGGACAAA 
ATCTACAGCA ATGCTGGCGA GAAGAGCTTT GACCGGCTCC TGCGTAAACT CCGGGAGCGG 
CTTCCCAAGG CCAGGGTTGT GGTCTGCTTC TGCGAGGGCA TGACAGTGCG GGGCTTACTG 
AGTGCCATGC GCCGCCTGGG CGTCGTGGGC GAGTTCTCAC TCATTGGAAG TGATGGATGG 
GCAGACAGAG ATGAAGTCAT CGAAGGCTAT GAGGTGGAAG CCAACGGAGG GATCACAATA 
AAGCTTCAGT CTCCAGAGGT CAGGTCATTT GATGACTACT TCCTGAAGCT GAGGCTGGAC 
ACCAACACAA GGAATCCTTG GTTCCCTGAG TTCTGGCAAC ATCGCTTCCA GTGTCGCCTA 
CCTGGACACC TCTTGGAAAA CCCCAACTTT AAGAAAGTGT GCACAGGAAA TGAAAGCTTG 
GAAGAAAACT ATGTCCAGGA CAGCAAAATG GGATTTGTCA TCAATGCCAT CTATGCCATG 
GCACATGGGC TGCAGAACAT GCACCATGCT CTGTGTCCCG GCCATGTGGG CCTGTGTGAT 
GCTATGAAAC CCATTGATGG CAGGAAGCTC CTGGATTTCC TCATCAAATC CTCTTTTGTC 
GGAGTGTCTG GAGAGGAGGT GTGGTTCGAT GAGAAGGGGG ATGCTCCCGG AAGGTATGAC 
ATTATGAATC TGCAGTACAC AGAAGCTAAT CGCTATGACT ATGTCCACGT GGGGACCTGG 
CATGAAGGAG TGCTGAATAT TGATGATTAC AAAATCCAGA TGAACAAAAG CGGAATGGTA 
CGATCTGTGT GCAGTGAGCC TTGCTTAAAG GGTCAGATTA AGGTCATACG GAAAGGAGAA 
GTGAGCTGCT GCTGGATCTG CACGGCCTGC AAAGAGAATG AGTTTGTGCA GGACGAGTTC 
ACCTGCAGAG CCTGTGACCT GGGGTGGTGG CCCAACGCAG AGCTCACAGG CTGTGAGCCC 
ATTCCTGTCC GTTATCTTGA GTGGAGTGAC ATAGAATCTA TCATAGCCAT CGCCTTTTCT 
TGCCTGGGCA TCCTCGTGAC GCTGTTTGTC ACCCTCATCT TCGTTCTGTA CCGGGACACA 
CCCGTGGTCA AATCCTCCAG TAGGGAGCTC TGCTATATCA TTCTGGCTGG TATTTTCCTC 
GGCTATGTGT GCCCTTTCAC CCTCATCGCC AAACCTACTA CCACATCCTG CTACCTCCAG 
CGCCTCCTAG TTGGCCTCTC TTCTGCCATG TGCTACTCTG CTTTAGTGAC CAAAACCAAT 
CGTATTGCAC GCATCCTGGC TGGCAGCAAG AAGAAGATCT GCACCCGGAA GCCCAGATTC 
ATGAGCGCTT GGGCCCAAGT GATCATAGCC TCCATTCTGA TTAGTGTACA GCTAACACTA 
GTGGTGACCT TGATCATCAT GGAGCCTCCC ATGCCCATTT TGTCCTACCC GAGTATCAAG 
GAAGTCTACC TTATCTGCAA TACCAGCAAC CTGGGTGTAG TGGCCCCTGT GGGTTACAAT 
GGACTCCTCA TCATGAGCTG TACCTACTAT GCCTTCAAGA CCCGCAACGT GCCGGCCAAC 
TTCAATGAGG CTAAATACAT CGCCTTCACC ATGTACACTA CCTGCATCAT CTGGCTGGCT 
TTCGTTCCCA TTTACTTTGG GAGCAACTAC AAGATCATCA CTACCTGCTT CGCGGTGAGC 
CTCAGTGTGA CGGTGGCCCT GGGGTGCATG TTTACTCCGA AGATGTACAT CATCATTGCC 
AAACCTGAGA GGAACGTCCG CAGTGCCTTC ACGACCTCTG ATGTTGTCCG CATGCACGTC 
GGTGATGGCA AACTGCCGTG CCGCTCCAAC ACCTTCCTCA ACATTTTCCG GAGAAAGAAG 
CCCGGGGCAG GGAATGCCAA TTCTAACGGC AAGTCTGTGT CATGGTCTGA ACCAGGTGGA 
AGACAGGCGC CCAAGGGACA GCACGTGTGG CAGCGCCTCT CTGTGCACGT GAAGACCAAC 
GAGACGGCCT GTAACCAAAC AGCCGTAATC AAACCCCTCA CTAAAAGTTA CCAAGGCTCT 
GGCAAGAGCC TGACCTTTTC AGATGCCAGC ACCAAGACCC TTTACAATGT GGAAGAAGAG 
GACAATACCC CTTCTGCTCA CTTCAGCCCT CCCAGCAGCC CTTCTATGGT GGTGCACCGA 
CGCGGGCCAC CCGTGGCCAC CACACCACCT CTGCCACCCC ATCTGACCGC AGAAGAGACC 
CCCCTGTTCC TGGCTGATTC CGTCATCCCC AAGGGCTTGC CTCCTCCTCT CCCGCAGCAG 
CAGCCACAGC AGCCGCCCCC TCAGCAGCCC CCGCAGCAGC CCAAGTCCCT GATGGACCAG 
CTGCAAGGCG TAGTCACCAA CTTCGGTTCG GGGATTCCAG ATTTCCATGC GGTGCTGGCA 
GGCCCGGGGA CACCAGGAAA CAGCCTGCGC TCTCTGTACC CGCCCCCGCC TCCGCCGCAA 
CACCTGCAGA TGCTGCCCCT GCACCTGAGC ACCTTCCAGG AGGAGTCCAT CTCCCCTCCT 
GGGGAGGACA TCGATGATGA CAGTGAGAGA TTCAAGCTCC TGCAGGAGTT CGTGTACGAG 
CGCGAAGGGA ACACCGAAGA AGATGAATTG GAAGAGGAGG AGGACCTGCC CACAGCCAGC 
AAGCTGACCC CTGAGGATTC TCCTGCCCTG ACGCCTCCTT CTCCTTTCCG AGATTCCGTG 
GCCTCTGGCA GCTCAGTGCC CAGTTCCCCC GTATCTGAGT CGGTCCTCTG CACCCCTCCA 
AATGTAACCT ACGCCTCTGT CATTCTGAGG GACTACAAGC AAAGCTCTTC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058707.1
CDS	377..3976
Translation	MVRLLLIFFPMIFLEMSILPRMPDRKVLLAGASSQRSVARMDGDVIIGALFSVHHQPPAEKVPERKCGEIREQYGIQRVEAMFHTLDKINADPVLLPNITLGSEIRDSCWHSSVALEQSIEFIRDSLISIRDEKDGLNRCLPDGQTLPPGRTKKPIAGVIGPGSSSVAIQVQNLLQLFDIPQIAYSATSIDLSDKTLYKYFLRVVPSDTLQARAMLDIVKRYNWTYVSAVHTEGNYGESGMDAFKELAAQEGLCIAHSDKIYSNAGEKSFDRLLRKLRERLPKARVVVCFCEGMTVRGLLSAMRRLGVVGEFSLIGSDGWADRDEVIEGYEVEANGGITIKLQSPEVRSFDDYFLKLRLDTNTRNPWFPEFWQHRFQCRLPGHLLENPNFKKVCTGNESLEENYVQDSKMGFVINAIYAMAHGLQNMHHALCPGHVGLCDAMKPIDGRKLLDFLIKSSFVGVSGEEVWFDEKGDAPGRYDIMNLQYTEANRYDYVHVGTWHEGVLNIDDYKIQMNKSGMVRSVCSEPCLKGQIKVIRKGEVSCCWICTACKENEFVQDEFTCRACDLGWWPNAELTGCEPIPVRYLEWSDIESIIAIAFSCLGILVTLFVTLIFVLYRDTPVVKSSSRELCYIILAGIFLGYVCPFTLIAKPTTTSCYLQRLLVGLSSAMCYSALVTKTNRIARILAGSKKKICTRKPRFMSAWAQVIIASILISVQLTLVVTLIIMEPPMPILSYPSIKEVYLICNTSNLGVVAPVGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTCFAVSLSVTVALGCMFTPKMYIIIAKPERNVRSAFTTSDVVRMHVGDGKLPCRSNTFLNIFRRKKPGAGNANSNGKSVSWSEPGGRQAPKGQHVWQRLSVHVKTNETACNQTAVIKPLTKSYQGSGKSLTFSDASTKTLYNVEEEDNTPSAHFSPPSSPSMVVHRRGPPVATTPPLPPHLTAEETPLFLADSVIPKGLPPPLPQQQPQQPPPQQPPQQPKSLMDQLQGVVTNFGSGIPDFHAVLAGPGTPGNSLRSLYPPPPPPQHLQMLPLHLSTFQEESISPPGEDIDDDSERFKLLQEFVYEREGNTEEDELEEEEDLPTASKLTPEDSPALTPPSPFRDSVASGSSVPSSPVSESVLCTPPNVTYASVILRDYKQSSSTL

Target ORF information:

RefSeq Version	NM_017011.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate metabotropic receptor 1 (Grm1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017011.1

ORF Insert Sequence:

ATGGTCCGGC TCCTCTTGAT TTTCTTCCCA ATGATCTTTT TGGAGATGTC CATTTTGCCC 
AGGATGCCTG ACAGAAAAGT ATTGCTGGCA GGTGCCTCGT CCCAGCGCTC CGTGGCGAGA 
ATGGACGGAG ATGTCATCAT CGGAGCCCTC TTCTCAGTCC ATCACCAGCC TCCAGCCGAG 
AAGGTACCCG AAAGGAAGTG TGGGGAGATC AGGGAACAGT ATGGTATCCA GAGGGTGGAG 
GCCATGTTCC ACACGTTGGA TAAGATTAAC GCGGACCCGG TGCTCCTGCC CAACATCACT 
CTGGGCAGTG AGATCCGGGA CTCCTGCTGG CACTCTTCAG TGGCTCTCGA ACAGAGCATC 
GAATTCATCA GAGACTCCCT GATTTCCATC CGAGATGAGA AGGATGGGCT GAACCGATGC 
CTGCCTGATG GCCAGACCCT GCCCCCTGGC AGGACTAAGA AGCCTATTGC TGGAGTGATC 
GGCCCTGGCT CCAGCTCTGT GGCCATTCAA GTCCAGAATC TTCTCCAGCT GTTCGACATC 
CCACAGATCG CCTATTCTGC CACAAGCATA GACCTGAGTG ACAAAACTTT GTACAAATAC 
TTCCTGAGGG TGGTCCCTTC TGACACTTTG CAGGCAAGGG CGATGCTCGA CATAGTCAAG 
CGTTACAACT GGACCTATGT CTCAGCAGTC CACACAGAAG GGAATTACGG CGAGAGTGGA 
ATGGATGCTT TCAAAGAACT GGCTGCCCAG GAAGGCCTCT GCATCGCACA CTCGGACAAA 
ATCTACAGCA ATGCTGGCGA GAAGAGCTTT GACCGGCTCC TGCGTAAACT CCGGGAGCGG 
CTTCCCAAGG CCAGGGTTGT GGTCTGCTTC TGCGAGGGCA TGACAGTGCG GGGCTTACTG 
AGTGCCATGC GCCGCCTGGG CGTCGTGGGC GAGTTCTCAC TCATTGGAAG TGATGGATGG 
GCAGACAGAG ATGAAGTCAT CGAAGGCTAT GAGGTGGAAG CCAACGGAGG GATCACAATA 
AAGCTTCAGT CTCCAGAGGT CAGGTCATTT GATGACTACT TCCTGAAGCT GAGGCTGGAC 
ACCAACACAA GGAATCCTTG GTTCCCTGAG TTCTGGCAAC ATCGCTTCCA GTGTCGCCTA 
CCTGGACACC TCTTGGAAAA CCCCAACTTT AAGAAAGTGT GCACAGGAAA TGAAAGCTTG 
GAAGAAAACT ATGTCCAGGA CAGCAAAATG GGATTTGTCA TCAATGCCAT CTATGCCATG 
GCACATGGGC TGCAGAACAT GCACCATGCT CTGTGTCCCG GCCATGTGGG CCTGTGTGAT 
GCTATGAAAC CCATTGATGG CAGGAAGCTC CTGGATTTCC TCATCAAATC CTCTTTTGTC 
GGAGTGTCTG GAGAGGAGGT GTGGTTCGAT GAGAAGGGGG ATGCTCCCGG AAGGTATGAC 
ATTATGAATC TGCAGTACAC AGAAGCTAAT CGCTATGACT ATGTCCACGT GGGGACCTGG 
CATGAAGGAG TGCTGAATAT TGATGATTAC AAAATCCAGA TGAACAAAAG CGGAATGGTA 
CGATCTGTGT GCAGTGAGCC TTGCTTAAAG GGTCAGATTA AGGTCATACG GAAAGGAGAA 
GTGAGCTGCT GCTGGATCTG CACGGCCTGC AAAGAGAATG AGTTTGTGCA GGACGAGTTC 
ACCTGCAGAG CCTGTGACCT GGGGTGGTGG CCCAACGCAG AGCTCACAGG CTGTGAGCCC 
ATTCCTGTCC GTTATCTTGA GTGGAGTGAC ATAGAATCTA TCATAGCCAT CGCCTTTTCT 
TGCCTGGGCA TCCTCGTGAC GCTGTTTGTC ACCCTCATCT TCGTTCTGTA CCGGGACACA 
CCCGTGGTCA AATCCTCCAG TAGGGAGCTC TGCTATATCA TTCTGGCTGG TATTTTCCTC 
GGCTATGTGT GCCCTTTCAC CCTCATCGCC AAACCTACTA CCACATCCTG CTACCTCCAG 
CGCCTCCTAG TTGGCCTCTC TTCTGCCATG TGCTACTCTG CTTTAGTGAC CAAAACCAAT 
CGTATTGCAC GCATCCTGGC TGGCAGCAAG AAGAAGATCT GCACCCGGAA GCCCAGATTC 
ATGAGCGCTT GGGCCCAAGT GATCATAGCC TCCATTCTGA TTAGTGTACA GCTAACACTA 
GTGGTGACCT TGATCATCAT GGAGCCTCCC ATGCCCATTT TGTCCTACCC GAGTATCAAG 
GAAGTCTACC TTATCTGCAA TACCAGCAAC CTGGGTGTAG TGGCCCCTGT GGGTTACAAT 
GGACTCCTCA TCATGAGCTG TACCTACTAT GCCTTCAAGA CCCGCAACGT GCCGGCCAAC 
TTCAATGAGG CTAAATACAT CGCCTTCACC ATGTACACTA CCTGCATCAT CTGGCTGGCT 
TTCGTTCCCA TTTACTTTGG GAGCAACTAC AAGATCATCA CTACCTGCTT CGCGGTGAGC 
CTCAGTGTGA CGGTGGCCCT GGGGTGCATG TTTACTCCGA AGATGTACAT CATCATTGCC 
AAACCTGAGA GGAACGTCCG CAGTGCCTTC ACGACCTCTG ATGTTGTCCG CATGCACGTC 
GGTGATGGCA AACTGCCGTG CCGCTCCAAC ACCTTCCTCA ACATTTTCCG GAGAAAGAAG 
CCCGGGGCAG GGAATGCCAA TTCTAACGGC AAGTCTGTGT CATGGTCTGA ACCAGGTGGA 
AGACAGGCGC CCAAGGGACA GCACGTGTGG CAGCGCCTCT CTGTGCACGT GAAGACCAAC 
GAGACGGCCT GTAACCAAAC AGCCGTAATC AAACCCCTCA CTAAAAGTTA CCAAGGCTCT 
GGCAAGAGCC TGACCTTTTC AGATGCCAGC ACCAAGACCC TTTACAATGT GGAAGAAGAG 
GACAATACCC CTTCTGCTCA CTTCAGCCCT CCCAGCAGCC CTTCTATGGT GGTGCACCGA 
CGCGGGCCAC CCGTGGCCAC CACACCACCT CTGCCACCCC ATCTGACCGC AGAAGAGACC 
CCCCTGTTCC TGGCTGATTC CGTCATCCCC AAGGGCTTGC CTCCTCCTCT CCCGCAGCAG 
CAGCCACAGC AGCCGCCCCC TCAGCAGCCC CCGCAGCAGC CCAAGTCCCT GATGGACCAG 
CTGCAAGGCG TAGTCACCAA CTTCGGTTCG GGGATTCCAG ATTTCCATGC GGTGCTGGCA 
GGCCCGGGGA CACCAGGAAA CAGCCTGCGC TCTCTGTACC CGCCCCCGCC TCCGCCGCAA 
CACCTGCAGA TGCTGCCCCT GCACCTGAGC ACCTTCCAGG AGGAGTCCAT CTCCCCTCCT 
GGGGAGGACA TCGATGATGA CAGTGAGAGA TTCAAGCTCC TGCAGGAGTT CGTGTACGAG 
CGCGAAGGGA ACACCGAAGA AGATGAATTG GAAGAGGAGG AGGACCTGCC CACAGCCAGC 
AAGCTGACCC CTGAGGATTC TCCTGCCCTG ACGCCTCCTT CTCCTTTCCG AGATTCCGTG 
GCCTCTGGCA GCTCAGTGCC CAGTTCCCCC GTATCTGAGT CGGTCCTCTG CACCCCTCCA 
AATGTAACCT ACGCCTCTGT CATTCTGAGG GACTACAAGC AAAGCTCTTC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grm1 ( XM_017588777.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444266.1 Rattus norvegicus glutamate receptor, metabotropic 1 (Grm1), transcript variant X1, mRNA.

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CloneID	ORa56381
Clone ID Related Accession (Same CDS sequence)	XM_017588777.1
Accession Version	XM_017588777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	metabotropic glutamate receptor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGGC TCCTCTTGAT TTTCTTCCCA ATGATCTTTT TGGAGATGTC CATTTTGCCC 
AGGATGCCTG ACAGAAAAGT ATTGCTGGCA GGTGCCTCGT CCCAGCGCTC CGTGGCGAGA 
ATGGACGGAG ATGTCATCAT CGGAGCCCTC TTCTCAGTCC ATCACCAGCC TCCAGCCGAG 
AAGGTACCCG AAAGGAAGTG TGGGGAGATC AGGGAACAGT ATGGTATCCA GAGGGTGGAG 
GCCATGTTCC ACACGTTGGA TAAGATTAAC GCGGACCCGG TGCTCCTGCC CAACATCACT 
CTGGGCAGTG AGATCCGGGA CTCCTGCTGG CACTCTTCAG TGGCTCTCGA ACAGAGCATC 
GAATTCATCA GAGACTCCCT GATTTCCATC CGAGATGAGA AGGATGGGCT GAACCGATGC 
CTGCCTGATG GCCAGACCCT GCCCCCTGGC AGGACTAAGA AGCCTATTGC TGGAGTGATC 
GGCCCTGGCT CCAGCTCTGT GGCCATTCAA GTCCAGAATC TTCTCCAGCT GTTCGACATC 
CCACAGATCG CCTATTCTGC CACAAGCATA GACCTGAGTG ACAAAACTTT GTACAAATAC 
TTCCTGAGGG TGGTCCCTTC TGACACTTTG CAGGCAAGGG CGATGCTCGA CATAGTCAAG 
CGTTACAACT GGACCTATGT CTCAGCAGTC CACACAGAAG GGAATTACGG CGAGAGTGGA 
ATGGATGCTT TCAAAGAACT GGCTGCCCAG GAAGGCCTCT GCATCGCACA CTCGGACAAA 
ATCTACAGCA ATGCTGGCGA GAAGAGCTTT GACCGGCTCC TGCGTAAACT CCGGGAGCGG 
CTTCCCAAGG CCAGGGTTGT GGTCTGCTTC TGCGAGGGCA TGACAGTGCG GGGCTTACTG 
AGTGCCATGC GCCGCCTGGG CGTCGTGGGC GAGTTCTCAC TCATTGGAAG TGATGGATGG 
GCAGACAGAG ATGAAGTCAT CGAAGGCTAT GAGGTGGAAG CCAACGGAGG GATCACAATA 
AAGCTTCAGT CTCCAGAGGT CAGGTCATTT GATGACTACT TCCTGAAGCT GAGGCTGGAC 
ACCAACACAA GGAATCCTTG GTTCCCTGAG TTCTGGCAAC ATCGCTTCCA GTGTCGCCTA 
CCTGGACACC TCTTGGAAAA CCCCAACTTT AAGAAAGTGT GCACAGGAAA TGAAAGCTTG 
GAAGAAAACT ATGTCCAGGA CAGCAAAATG GGATTTGTCA TCAATGCCAT CTATGCCATG 
GCACATGGGC TGCAGAACAT GCACCATGCT CTGTGTCCCG GCCATGTGGG CCTGTGTGAT 
GCTATGAAAC CCATTGATGG CAGGAAGCTC CTGGATTTCC TCATCAAATC CTCTTTTGTC 
GGAGTGTCTG GAGAGGAGGT GTGGTTCGAT GAGAAGGGGG ATGCTCCCGG AAGGTATGAC 
ATTATGAATC TGCAGTACAC AGAAGCTAAT CGCTATGACT ATGTCCACGT GGGGACCTGG 
CATGAAGGAG TGCTGAATAT TGATGATTAC AAAATCCAAA TGAACAAAAG CGGAATGGTA 
CGATCTGTGT GCAGTGAGCC TTGCTTAAAG GGTCAGATTA AGGTCATACG GAAAGGAGAA 
GTGAGCTGCT GCTGGATCTG CACGGCCTGC AAAGAGAATG AGTTTGTGCA GGACGAGTTC 
ACCTGCAGAG CCTGTGACCT GGGGTGGTGG CCCAACGCAG AGCTCACAGG CTGTGAGCCC 
ATTCCTGTCC GTTATCTTGA GTGGAGTGAC ATAGAATCTA TCATAGCCAT CGCCTTTTCT 
TGCCTGGGCA TCCTCGTGAC GCTGTTTGTC ACCCTCATCT TCGTTCTGTA CCGGGACACA 
CCCGTGGTCA AATCCTCCAG TAGGGAGCTC TGCTATATCA TTCTGGCTGG TATTTTCCTC 
GGCTATGTGT GCCCTTTCAC CCTCATCGCC AAACCTACTA CCACATCCTG CTACCTCCAG 
CGCCTCCTAG TTGGCCTCTC TTCTGCCATG TGCTACTCTG CTTTAGTGAC CAAAACCAAT 
CGTATTGCAC GCATCCTGGC TGGCAGCAAG AAGAAGATCT GCACCCGGAA GCCCAGATTC 
ATGAGCGCTT GGGCCCAAGT GATCATAGCC TCCATTCTGA TTAGTGTACA GCTAACACTA 
GTGGTGACCT TGATCATCAT GGAGCCTCCC ATGCCCATTT TGTCCTACCC GAGTATCAAG 
GAAGTCTACC TTATCTGCAA TACCAGCAAC CTGGGTGTAG TGGCCCCTGT GGGTTACAAT 
GGACTCCTCA TCATGAGCTG TACCTACTAT GCCTTCAAGA CCCGCAACGT GCCGGCCAAC 
TTCAATGAGG CTAAATACAT CGCCTTCACC ATGTACACTA CCTGCATCAT CTGGCTGGCT 
TTCGTTCCCA TTTACTTTGG GAGCAACTAC AAGATCATCA CTACCTGCTT CGCGGTGAGC 
CTCAGTGTGA CGGTGGCCCT GGGGTGCATG TTTACTCCGA AGATGTACAT CATCATTGCC 
AAACCTGAGA GGAACGTCCG CAGTGCCTTC ACGACCTCTG ATGTTGTCCG CATGCACGTC 
GGTGATGGCA AACTGCCGTG CCGCTCCAAC ACCTTCCTCA ACATTTTCCG GAGAAAGAAG 
CCCGGGGCAG GGAATGCCAA TTCTAACGGC AAGTCTGTGT CATGGTCTGA ACCAGGTGGA 
AGACAGGCGC CCAAGGGACA GCACGTGTGG CAGCGCCTCT CTGTGCACGT GAAGACCAAC 
GAGACGGCCT GTAACCAAAC AGCCGTAATC AAACCCCTCA CTAAAAGTTA CCAAGGCTCT 
GGCAAGAGCC TGACCTTTTC AGATGCCAGC ACCAAGACCC TTTACAATGT GGAAGAAGAG 
GACAATACCC CTTCTGCTCA CTTCAGCCCT CCCAGCAGCC CTTCTATGGT GGTGCACCGA 
CGCGGGCCAC CCGTGGCCAC CACACCACCT CTGCCACCCC ATCTGACCGC AGAAGAGACC 
CCCCTGTTCC TGGCTGATTC CGTCATCCCC AAGGGCTTGC CTCCTCCTCT CCCGCAGCAG 
CAGCCACAGC AGCCGCCCCC TCAGCAGCCC CCGCAGCAGC CCAAGTCCCT GATGGACCAG 
CTGCAAGGCG TAGTCACCAA CTTCGGTTCG GGGATTCCAG ATTTCCATGC GGTGCTGGCA 
GGCCCGGGGA CACCAGGAAA CAGCCTGCGC TCTCTGTACC CGCCCCCGCC TCCGCCGCAA 
CACCTGCAGA TGCTGCCCCT GCACCTGAGC ACCTTCCAGG AGGAGTCCAT CTCCCCTCCT 
GGGGAGGACG ACGATGATGA CAGTGAGAGA TTCAAGCTCC TGCAGGAGTT CGTGTACGAG 
CGCGAAGGGA ACACCGAAGA AGATGAATTG GAAGAGGAGG AGGACCTGCC CACAGCCAGC 
AAGCTGACCC CTGAGGATTC TCCTGCCCTG ACGCCTCCTT CTCCTTTCCG AGATTCCGTG 
GCCTCTGGCA GCTCAGTGCC CAGTTCCCCC GTATCTGAGT CGGTCCTCTG CACCCCTCCA 
AATGTGACCT ACGCCTCTGT CATTCTGAGG GACTACAAGC AAAGCTCTTC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444266.1
CDS	407..4006
Translation	MVRLLLIFFPMIFLEMSILPRMPDRKVLLAGASSQRSVARMDGDVIIGALFSVHHQPPAEKVPERKCGEIREQYGIQRVEAMFHTLDKINADPVLLPNITLGSEIRDSCWHSSVALEQSIEFIRDSLISIRDEKDGLNRCLPDGQTLPPGRTKKPIAGVIGPGSSSVAIQVQNLLQLFDIPQIAYSATSIDLSDKTLYKYFLRVVPSDTLQARAMLDIVKRYNWTYVSAVHTEGNYGESGMDAFKELAAQEGLCIAHSDKIYSNAGEKSFDRLLRKLRERLPKARVVVCFCEGMTVRGLLSAMRRLGVVGEFSLIGSDGWADRDEVIEGYEVEANGGITIKLQSPEVRSFDDYFLKLRLDTNTRNPWFPEFWQHRFQCRLPGHLLENPNFKKVCTGNESLEENYVQDSKMGFVINAIYAMAHGLQNMHHALCPGHVGLCDAMKPIDGRKLLDFLIKSSFVGVSGEEVWFDEKGDAPGRYDIMNLQYTEANRYDYVHVGTWHEGVLNIDDYKIQMNKSGMVRSVCSEPCLKGQIKVIRKGEVSCCWICTACKENEFVQDEFTCRACDLGWWPNAELTGCEPIPVRYLEWSDIESIIAIAFSCLGILVTLFVTLIFVLYRDTPVVKSSSRELCYIILAGIFLGYVCPFTLIAKPTTTSCYLQRLLVGLSSAMCYSALVTKTNRIARILAGSKKKICTRKPRFMSAWAQVIIASILISVQLTLVVTLIIMEPPMPILSYPSIKEVYLICNTSNLGVVAPVGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTCFAVSLSVTVALGCMFTPKMYIIIAKPERNVRSAFTTSDVVRMHVGDGKLPCRSNTFLNIFRRKKPGAGNANSNGKSVSWSEPGGRQAPKGQHVWQRLSVHVKTNETACNQTAVIKPLTKSYQGSGKSLTFSDASTKTLYNVEEEDNTPSAHFSPPSSPSMVVHRRGPPVATTPPLPPHLTAEETPLFLADSVIPKGLPPPLPQQQPQQPPPQQPPQQPKSLMDQLQGVVTNFGSGIPDFHAVLAGPGTPGNSLRSLYPPPPPPQHLQMLPLHLSTFQEESISPPGEDDDDDSERFKLLQEFVYEREGNTEEDELEEEEDLPTASKLTPEDSPALTPPSPFRDSVASGSSVPSSPVSESVLCTPPNVTYASVILRDYKQSSSTL

Target ORF information:

RefSeq Version	XM_017588777.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate receptor, metabotropic 1 (Grm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588777.1

ORF Insert Sequence:

ATGGTCCGGC TCCTCTTGAT TTTCTTCCCA ATGATCTTTT TGGAGATGTC CATTTTGCCC 
AGGATGCCTG ACAGAAAAGT ATTGCTGGCA GGTGCCTCGT CCCAGCGCTC CGTGGCGAGA 
ATGGACGGAG ATGTCATCAT CGGAGCCCTC TTCTCAGTCC ATCACCAGCC TCCAGCCGAG 
AAGGTACCCG AAAGGAAGTG TGGGGAGATC AGGGAACAGT ATGGTATCCA GAGGGTGGAG 
GCCATGTTCC ACACGTTGGA TAAGATTAAC GCGGACCCGG TGCTCCTGCC CAACATCACT 
CTGGGCAGTG AGATCCGGGA CTCCTGCTGG CACTCTTCAG TGGCTCTCGA ACAGAGCATC 
GAATTCATCA GAGACTCCCT GATTTCCATC CGAGATGAGA AGGATGGGCT GAACCGATGC 
CTGCCTGATG GCCAGACCCT GCCCCCTGGC AGGACTAAGA AGCCTATTGC TGGAGTGATC 
GGCCCTGGCT CCAGCTCTGT GGCCATTCAA GTCCAGAATC TTCTCCAGCT GTTCGACATC 
CCACAGATCG CCTATTCTGC CACAAGCATA GACCTGAGTG ACAAAACTTT GTACAAATAC 
TTCCTGAGGG TGGTCCCTTC TGACACTTTG CAGGCAAGGG CGATGCTCGA CATAGTCAAG 
CGTTACAACT GGACCTATGT CTCAGCAGTC CACACAGAAG GGAATTACGG CGAGAGTGGA 
ATGGATGCTT TCAAAGAACT GGCTGCCCAG GAAGGCCTCT GCATCGCACA CTCGGACAAA 
ATCTACAGCA ATGCTGGCGA GAAGAGCTTT GACCGGCTCC TGCGTAAACT CCGGGAGCGG 
CTTCCCAAGG CCAGGGTTGT GGTCTGCTTC TGCGAGGGCA TGACAGTGCG GGGCTTACTG 
AGTGCCATGC GCCGCCTGGG CGTCGTGGGC GAGTTCTCAC TCATTGGAAG TGATGGATGG 
GCAGACAGAG ATGAAGTCAT CGAAGGCTAT GAGGTGGAAG CCAACGGAGG GATCACAATA 
AAGCTTCAGT CTCCAGAGGT CAGGTCATTT GATGACTACT TCCTGAAGCT GAGGCTGGAC 
ACCAACACAA GGAATCCTTG GTTCCCTGAG TTCTGGCAAC ATCGCTTCCA GTGTCGCCTA 
CCTGGACACC TCTTGGAAAA CCCCAACTTT AAGAAAGTGT GCACAGGAAA TGAAAGCTTG 
GAAGAAAACT ATGTCCAGGA CAGCAAAATG GGATTTGTCA TCAATGCCAT CTATGCCATG 
GCACATGGGC TGCAGAACAT GCACCATGCT CTGTGTCCCG GCCATGTGGG CCTGTGTGAT 
GCTATGAAAC CCATTGATGG CAGGAAGCTC CTGGATTTCC TCATCAAATC CTCTTTTGTC 
GGAGTGTCTG GAGAGGAGGT GTGGTTCGAT GAGAAGGGGG ATGCTCCCGG AAGGTATGAC 
ATTATGAATC TGCAGTACAC AGAAGCTAAT CGCTATGACT ATGTCCACGT GGGGACCTGG 
CATGAAGGAG TGCTGAATAT TGATGATTAC AAAATCCAAA TGAACAAAAG CGGAATGGTA 
CGATCTGTGT GCAGTGAGCC TTGCTTAAAG GGTCAGATTA AGGTCATACG GAAAGGAGAA 
GTGAGCTGCT GCTGGATCTG CACGGCCTGC AAAGAGAATG AGTTTGTGCA GGACGAGTTC 
ACCTGCAGAG CCTGTGACCT GGGGTGGTGG CCCAACGCAG AGCTCACAGG CTGTGAGCCC 
ATTCCTGTCC GTTATCTTGA GTGGAGTGAC ATAGAATCTA TCATAGCCAT CGCCTTTTCT 
TGCCTGGGCA TCCTCGTGAC GCTGTTTGTC ACCCTCATCT TCGTTCTGTA CCGGGACACA 
CCCGTGGTCA AATCCTCCAG TAGGGAGCTC TGCTATATCA TTCTGGCTGG TATTTTCCTC 
GGCTATGTGT GCCCTTTCAC CCTCATCGCC AAACCTACTA CCACATCCTG CTACCTCCAG 
CGCCTCCTAG TTGGCCTCTC TTCTGCCATG TGCTACTCTG CTTTAGTGAC CAAAACCAAT 
CGTATTGCAC GCATCCTGGC TGGCAGCAAG AAGAAGATCT GCACCCGGAA GCCCAGATTC 
ATGAGCGCTT GGGCCCAAGT GATCATAGCC TCCATTCTGA TTAGTGTACA GCTAACACTA 
GTGGTGACCT TGATCATCAT GGAGCCTCCC ATGCCCATTT TGTCCTACCC GAGTATCAAG 
GAAGTCTACC TTATCTGCAA TACCAGCAAC CTGGGTGTAG TGGCCCCTGT GGGTTACAAT 
GGACTCCTCA TCATGAGCTG TACCTACTAT GCCTTCAAGA CCCGCAACGT GCCGGCCAAC 
TTCAATGAGG CTAAATACAT CGCCTTCACC ATGTACACTA CCTGCATCAT CTGGCTGGCT 
TTCGTTCCCA TTTACTTTGG GAGCAACTAC AAGATCATCA CTACCTGCTT CGCGGTGAGC 
CTCAGTGTGA CGGTGGCCCT GGGGTGCATG TTTACTCCGA AGATGTACAT CATCATTGCC 
AAACCTGAGA GGAACGTCCG CAGTGCCTTC ACGACCTCTG ATGTTGTCCG CATGCACGTC 
GGTGATGGCA AACTGCCGTG CCGCTCCAAC ACCTTCCTCA ACATTTTCCG GAGAAAGAAG 
CCCGGGGCAG GGAATGCCAA TTCTAACGGC AAGTCTGTGT CATGGTCTGA ACCAGGTGGA 
AGACAGGCGC CCAAGGGACA GCACGTGTGG CAGCGCCTCT CTGTGCACGT GAAGACCAAC 
GAGACGGCCT GTAACCAAAC AGCCGTAATC AAACCCCTCA CTAAAAGTTA CCAAGGCTCT 
GGCAAGAGCC TGACCTTTTC AGATGCCAGC ACCAAGACCC TTTACAATGT GGAAGAAGAG 
GACAATACCC CTTCTGCTCA CTTCAGCCCT CCCAGCAGCC CTTCTATGGT GGTGCACCGA 
CGCGGGCCAC CCGTGGCCAC CACACCACCT CTGCCACCCC ATCTGACCGC AGAAGAGACC 
CCCCTGTTCC TGGCTGATTC CGTCATCCCC AAGGGCTTGC CTCCTCCTCT CCCGCAGCAG 
CAGCCACAGC AGCCGCCCCC TCAGCAGCCC CCGCAGCAGC CCAAGTCCCT GATGGACCAG 
CTGCAAGGCG TAGTCACCAA CTTCGGTTCG GGGATTCCAG ATTTCCATGC GGTGCTGGCA 
GGCCCGGGGA CACCAGGAAA CAGCCTGCGC TCTCTGTACC CGCCCCCGCC TCCGCCGCAA 
CACCTGCAGA TGCTGCCCCT GCACCTGAGC ACCTTCCAGG AGGAGTCCAT CTCCCCTCCT 
GGGGAGGACG ACGATGATGA CAGTGAGAGA TTCAAGCTCC TGCAGGAGTT CGTGTACGAG 
CGCGAAGGGA ACACCGAAGA AGATGAATTG GAAGAGGAGG AGGACCTGCC CACAGCCAGC 
AAGCTGACCC CTGAGGATTC TCCTGCCCTG ACGCCTCCTT CTCCTTTCCG AGATTCCGTG 
GCCTCTGGCA GCTCAGTGCC CAGTTCCCCC GTATCTGAGT CGGTCCTCTG CACCCCTCCA 
AATGTGACCT ACGCCTCTGT CATTCTGAGG GACTACAAGC AAAGCTCTTC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transient Switching of NMDA-Dependent Long-Term Synaptic Potentiation in CA3-CA1 Hippocampal Synapses to mGluR
Cellular and molecular neurobiology39(2)287-300(2019 Mar)
Postnikova TY,Trofimova AM,Ergina JL,Zubareva OE,Kalemenev SV,Zaitsev AV

Amphetamine-induced Conditioned Place Preference and Changes in mGlu1/5 Receptor Expression and Signaling in the Rat Medial Prefrontal Cortex.
Neuroscience400110-119(2019 02)
Mao LM,Wang JQ

The Probability of Neurotransmitter Release Governs AMPA Receptor Trafficking via Activity-Dependent Regulation of mGluR1 Surface Expression.
Cell reports25(13)3631-3646.e3(2018 12)
Sanderson TM,Bradley CA,Georgiou J,Hong YH,Ng AN,Lee Y,Kim HD,Kim D,Amici M,Son GH,Zhuo M,Kim K,Kaang BK,Kim SJ,Collingridge GL

In Vivo Monitoring for Regional Changes of Metabotropic Glutamate Receptor Subtype 1 (mGluR1) in Pilocarpine-Induced Epileptic Rat Brain by Small-Animal PET.
Scientific reports7(1)14945(2017 11)
Yamasaki T,Fujinaga M,Mori W,Zhang Y,Wakizaka H,Nengaki N,Xie L,Hatori A,Zhang MR

Effects of GABACR and mGluR1 antagonists on contrast response functions of Sprague-Dawley and P23H rat retinal ganglion cells.
PloS one12(12)e0189980(2017)
Jensen R

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Grm1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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