A3galt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	A3galt2
Entrez Gene ID	171553
Full Name	alpha 1,3-galactosyltransferase 2
General protein information	Preferred Names alpha 1,3-galactosyltransferase 2 Names alpha-1,3-galactosyltransferase 2 iGb3 synthase iGb3S isoglobotriaosylceramide synthase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	UDP-galactose: beta-d-galactosyl-1,4-glucosylceramide alpha-1, 3-galactosyltransferase; involved in the synthesis of the isoglobo-series of glycosphingolipids [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138524.2	NP_612533.1	alpha-1,3-galactosyltransferase 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	A3GALT2	XP_544424.3
Homo sapiens (human)	A3GALT2	NP_001073907.1
Macaca mulatta (Rhesus monkey)	LOC711045	XP_001099773.2
Bos taurus (cattle)	A3GALT2	XP_005203105.1
Mus musculus (house mouse)	A3galt2	NP_001009819.1
Rattus norvegicus (Norway rat)	A3galt2	NP_612533.1

Gene Ontology
Bibliography

Related articles in PubMed

Globosides but not isoglobosides can impact the development of invariant NKT cells and their interaction with dendritic cells.
Porubsky S, Speak AO, Salio M, Jennemann R, Bonrouhi M, Zafarulla R, Singh Y, Dyson J, Luckow B, Lehuen A, Malle E, Müthing J, Platt FM, Cerundolo V, Gröne HJ
Journal of immunology (Baltimore, Md. : 1950)189(6)3007-17(2012 Sep)

Humans lack iGb3 due to the absence of functional iGb3-synthase: implications for NKT cell development and transplantation.
Christiansen D, Milland J, Mouhtouris E, Vaughan H, Pellicci DG, McConville MJ, Godfrey DI, Sandrin MS
PLoS biology6(7)e172(2008 Jul)

Enzymes that synthesize the IB4 epitope are not sufficient to impart IB4 binding in dorsal root ganglia of rat.
Fullmer JM, Riedl M, Williams FG, Sandrin M, Elde R
The Journal of comparative neurology501(1)70-82(2007 Mar)

Characterization of the rat alpha(1,3)galactosyltransferase: evidence for two independent genes encoding glycosyltransferases that synthesize Galalpha(1,3)Gal by two separate glycosylation pathways.
Taylor SG, McKenzie IF, Sandrin MS
Glycobiology13(5)327-37(2003 May)

Expression cloning of a new member of the ABO blood group glycosyltransferases, iGb3 synthase, that directs the synthesis of isoglobo-glycosphingolipids.
Keusch JJ, Manzella SM, Nyame KA, Cummings RD, Baenziger JU
The Journal of biological chemistry275(33)25308-14(2000 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following A3galt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the A3galt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12577	NM_138524.2 Latest version!	Rattus norvegicus alpha 1,3-galactosyltransferase 2 (A3galt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

A3galt2 ( NM_138524.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_612533.1 Rattus norvegicus alpha 1,3-galactosyltransferase 2 (A3galt2), mRNA.

Price & Availability↑

CloneID	ORa12577
Clone ID Related Accession (Same CDS sequence)	NM_138524.2
Accession Version	NM_138524.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Rattus norvegicus(Norway rat)
Product	alpha-1,3-galactosyltransferase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC061714.1. On Dec 20, 2003 this sequence version replaced NM_138524.1. ##Evidence-Data-START## Transcript exon combination :: BC061714.1, AF248543.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689596 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCTGG AGGGACTCAG GGCCAAGAAG AGACTCCTGT GGCGACTCTT CCTGTCTGCG 
TTTGGTCTCC TAGGCCTGTA CCATTACTGG TTCAAGATTT TCAGGCTCTT TGAAGTGTTC 
ATCCCCATGG GCATCTGCCC TATGGCCATC ATGCCTCTGC TAAAGGACAA TTTCACCGGA 
GTGCTGCGTC ACTGGGCCCG GCCTGAAGTC CTGACCTGTA CCTCTTGGGG AGCCCCAATT 
ATCTGGGATG AGACCTTCGA CCCACACGTA GCTGAGCGAG AGGCGAGACG GCAGAACCTT 
ACCATTGGAC TGACTGTCTT TGCGGTAGGC AGGTACCTGG AGAAGTACCT GGAGCACTTC 
CTGGTATCGG CAGAGCAGTA CTTCATGGTC GGCCAGAACG TGGTGTATTA TGTGTTTACC 
GACCGACCGG AAGCAGTGCC CCACGTGGCT CTAGGCCAGG GTCGCCTGCT GCGGGTGAAA 
CCAGTGAGAA GAGAGAAGCG CTGGCAGGAC GTGTCAATGG CTCGCATGCT CACGCTACAC 
GAGGCTCTGG GAGGGCAGCT GGGCCGAGAA GCTGACTATG TGTTCTGCCT GGACGTGGAC 
CAGTACTTCA GCGGTAACTT CGGACCCGAG GTGCTGGCAG ATTTGGTGGC ACAGCTGCAC 
GCCTGGCACT TCCGCTGGCC TCGGTGGATG CTGCCCTACG AGAGGGACAA GCGGTCGGCT 
GCCGCGCTGT CGTTAAGCGA AGGCGATTTC TACTACCACG CCGCGGTGTT CGGGGGCAGT 
GTGGCGGCTC TGCTCAAGCT GACGGCTCAC TGTGCGACTG GCCAACAGCT GGACCGTGAG 
CATGGCATTG AGGCACGCTG GCACGACGAG AGCCACCTCA ACAAGTTCTT CTGGCTGAGC 
AAGCCCACCA AGCTGCTGTC GCCAGAGTTT TGCTGGGCAG AGGAAATTGG CTGGAGGCCA 
GAGATCCACC ACCCTCGTCT GATCTGGGCA CCCAAGGAGT ACGCACTGGT GCGAACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612533.1
CDS	78..1097
Translation	MALEGLRAKKRLLWRLFLSAFGLLGLYHYWFKIFRLFEVFIPMGICPMAIMPLLKDNFTGVLRHWARPEVLTCTSWGAPIIWDETFDPHVAEREARRQNLTIGLTVFAVGRYLEKYLEHFLVSAEQYFMVGQNVVYYVFTDRPEAVPHVALGQGRLLRVKPVRREKRWQDVSMARMLTLHEALGGQLGREADYVFCLDVDQYFSGNFGPEVLADLVAQLHAWHFRWPRWMLPYERDKRSAAALSLSEGDFYYHAAVFGGSVAALLKLTAHCATGQQLDREHGIEARWHDESHLNKFFWLSKPTKLLSPEFCWAEEIGWRPEIHHPRLIWAPKEYALVRT

Target ORF information:

RefSeq Version	NM_138524.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alpha 1,3-galactosyltransferase 2 (A3galt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138524.2

ORF Insert Sequence:

ATGGCTCTGG AGGGACTCAG GGCCAAGAAG AGACTCCTGT GGCGACTCTT CCTGTCTGCG 
TTTGGTCTCC TAGGCCTGTA CCATTACTGG TTCAAGATTT TCAGGCTCTT TGAAGTGTTC 
ATCCCCATGG GCATCTGCCC TATGGCCATC ATGCCTCTGC TAAAGGACAA TTTCACCGGA 
GTGCTGCGTC ACTGGGCCCG GCCTGAAGTC CTGACCTGTA CCTCTTGGGG AGCCCCAATT 
ATCTGGGATG AGACCTTCGA CCCACACGTA GCTGAGCGAG AGGCGAGACG GCAGAACCTT 
ACCATTGGAC TGACTGTCTT TGCGGTAGGC AGGTACCTGG AGAAGTACCT GGAGCACTTC 
CTGGTATCGG CAGAGCAGTA CTTCATGGTC GGCCAGAACG TGGTGTATTA TGTGTTTACC 
GACCGACCGG AAGCAGTGCC CCACGTGGCT CTAGGCCAGG GTCGCCTGCT GCGGGTGAAA 
CCAGTGAGAA GAGAGAAGCG CTGGCAGGAC GTGTCAATGG CTCGCATGCT CACGCTACAC 
GAGGCTCTGG GAGGGCAGCT GGGCCGAGAA GCTGACTATG TGTTCTGCCT GGACGTGGAC 
CAGTACTTCA GCGGTAACTT CGGACCCGAG GTGCTGGCAG ATTTGGTGGC ACAGCTGCAC 
GCCTGGCACT TCCGCTGGCC TCGGTGGATG CTGCCCTACG AGAGGGACAA GCGGTCGGCT 
GCCGCGCTGT CGTTAAGCGA AGGCGATTTC TACTACCACG CCGCGGTGTT CGGGGGCAGT 
GTGGCGGCTC TGCTCAAGCT GACGGCTCAC TGTGCGACTG GCCAACAGCT GGACCGTGAG 
CATGGCATTG AGGCACGCTG GCACGACGAG AGCCACCTCA ACAAGTTCTT CTGGCTGAGC 
AAGCCCACCA AGCTGCTGTC GCCAGAGTTT TGCTGGGCAG AGGAAATTGG CTGGAGGCCA 
GAGATCCACC ACCCTCGTCT GATCTGGGCA CCCAAGGAGT ACGCACTGGT GCGAACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Globosides but not isoglobosides can impact the development of invariant NKT cells and their interaction with dendritic cells.
Journal of immunology (Baltimore, Md. : 1950)189(6)3007-17(2012 Sep)
Porubsky S,Speak AO,Salio M,Jennemann R,Bonrouhi M,Zafarulla R,Singh Y,Dyson J,Luckow B,Lehuen A,Malle E,Müthing J,Platt FM,Cerundolo V,Gröne HJ

Humans lack iGb3 due to the absence of functional iGb3-synthase: implications for NKT cell development and transplantation.
PLoS biology6(7)e172(2008 Jul)
Christiansen D,Milland J,Mouhtouris E,Vaughan H,Pellicci DG,McConville MJ,Godfrey DI,Sandrin MS

Enzymes that synthesize the IB4 epitope are not sufficient to impart IB4 binding in dorsal root ganglia of rat.
The Journal of comparative neurology501(1)70-82(2007 Mar)
Fullmer JM,Riedl M,Williams FG,Sandrin M,Elde R

Characterization of the rat alpha(1,3)galactosyltransferase: evidence for two independent genes encoding glycosyltransferases that synthesize Galalpha(1,3)Gal by two separate glycosylation pathways.
Glycobiology13(5)327-37(2003 May)
Taylor SG,McKenzie IF,Sandrin MS

Expression cloning of a new member of the ABO blood group glycosyltransferases, iGb3 synthase, that directs the synthesis of isoglobo-glycosphingolipids.
The Journal of biological chemistry275(33)25308-14(2000 Aug)
Keusch JJ,Manzella SM,Nyame KA,Cummings RD,Baenziger JU