Ehhadh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ehhadh
Entrez Gene ID	171142
Full Name	enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase
Synonyms	Lbp,MEF,Mfe,Mfe1,Pbe,Pbfe,pe-CoA,perMFE-1
General protein information	Preferred Names enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase Names peroxisomal bifunctional enzyme L-specific bifunctional protein enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase multifunctional enzyme type 1 peroxisomal bifunctional enzyme type 1 peroxisomal enoyl-CoA/hydrotase-3-hydroxyacyl-CoA
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q23
Summary	enzyme involved in the B-oxidation cycle of peroxisomes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133606.1	NP_598290.1	peroxisomal bifunctional enzyme

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	EHHADH	XP_001096204.1
Bos taurus (cattle)	EHHADH	NP_001069248.2
Mus musculus (house mouse)	Ehhadh	NP_076226.2
Gallus gallus (chicken)	EHHADH	XP_422690.2
Arabidopsis thaliana (thale cress)	AIM1	NP_194630.1
Oryza sativa (rice)	Os02g0274100	NP_001046536.1
Rattus norvegicus (Norway rat)	Ehhadh	NP_598290.1
Xenopus tropicalis (tropical clawed frog)	ehhadh	XP_002936579.1
Pan troglodytes (chimpanzee)	EHHADH	XP_517004.2
Canis lupus familiaris (dog)	EHHADH	XP_545234.1
Homo sapiens (human)	EHHADH	NP_001957.2
Danio rerio (zebrafish)	ehhadh	NP_996951.1

Gene Ontology
Bibliography

Related articles in PubMed

The isomerase and hydratase reaction mechanism of the crotonase active site of the multifunctional enzyme (type-1), as deduced from structures of complexes with 3S-hydroxy-acyl-CoA.
Kasaragod P, Schmitz W, Hiltunen JK, Wierenga RK
The FEBS journal280(13)3160-75(2013 Jul)

Crystal structure of liganded rat peroxisomal multifunctional enzyme type 1: a flexible molecule with two interconnected active sites.
Kasaragod P, Venkatesan R, Kiema TR, Hiltunen JK, Wierenga RK
The Journal of biological chemistry285(31)24089-98(2010 Jul)

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Ghosh D, Berg JM
Journal of the American Chemical Society132(11)3973-9(2010 Mar)

Regulation of cellular metabolism by protein lysine acetylation.
Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL
Science (New York, N.Y.)327(5968)1000-4(2010 Feb)

Beta-oxidation in hepatocyte cultures from mice with peroxisomal gene knockouts.
Dirkx R, Meyhi E, Asselberghs S, Reddy J, Baes M, Van Veldhoven PP
Biochemical and biophysical research communications357(3)718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ehhadh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ehhadh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01058	NM_133606.1 Latest version!	Rattus norvegicus enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase (Ehhadh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ehhadh ( NM_133606.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_598290.1 Rattus norvegicus enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase (Ehhadh), mRNA.

Price & Availability↑

CloneID	ORa01058
Clone ID Related Accession (Same CDS sequence)	NM_133606.1
Accession Version	NM_133606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568908800000
Organism	Rattus norvegicus(Norway rat)
Product	peroxisomal bifunctional enzyme
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from K03249.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: K03249.1, BC089777.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689595 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGAGT ATCTGAGGCT GCCCCACTCC CTGGCGATGA TCCGCCTCTG CAACCCGCCA 
GTCAACGCCG TCAGTCCAAC TGTAATCAGG GAAGTAAGAA ATGGACTCCA GAAAGCTGGA 
TCAGACCACA CGGTTAAAGC CATTGTGATC TGTGGCGCAA ACGGAAACTT CTGTGCAGGT 
GCTGATATCC ATGGCTTTAG TGCTTTTACC CCTGGCCTTG CATTAGGAAG CTTGGTAGAT 
GAAATACAGA GATACCAGAA GCCGGTGTTG GCCGCTATCC AAGGTGTGGC TCTCGGAGGA 
GGACTGGAGC TGGCCTTGGG CTGTCACTAT CGGATTGCCA ATGCAAAGGC TCGTGTCGGC 
TTGCCAGAAG TCACACTGGG GATTCTTCCT GGTGCAAGAG GAACCCAGCT TCTCCCCAGG 
GTCGTTGGAG TTCCTGTTGC TCTTGACCTA ATTACCTCAG GAAAATATCT TTCAGCAGAT 
GAAGCACTCA GGCTTGGAAT TCTGGATGCA GTCGTGAAGT CAGACCCAGT TGAAGAAGCC 
ATCAAATTTG CTCAGAAGAT TATAGATAAA CCCATAGAAC CCCGCAGGAT CTTTAACAAG 
CCAGTTCCAA GCTTGCCCAA CATGGACAGT GTTTTTGCAG AAGCCATCGC CAAGGTACGA 
AAACAGTACC CTGGTGTCCT GGCTCCGGAG ACGTGTGTCC GCTCAATCCA GGCCTCTGTG 
AAGCATCCCT ACGAAGTAGG CATCAAGGAA GAGGAAAAGC TGTTTATGTA CCTCCGGGCA 
TCCGGGCAGG CTAAAGCCCT ACAGTATGCC TTCTTTGCTG AAAAGTCTGC AAATAAGTGG 
TCAACTCCCT CAGGAGCGTC TTGGAAAACA GCCTCTGCTC AACCCGTCTC CTCAGTTGGC 
GTTCTTGGCT TGGGAACGAT GGGCCGAGGC ATCGCCATTT CTTTTGCGAG AGTGGGGATC 
TCTGTGGTTG CTGTGGAGTC AGACCCAAAG CAGCTAGATG CTGCAAAGAA GATAATCACT 
TTCACCTTGG AGAAGGAAGC ATCCAGAGCG CATCAGAACG GCCAAGCTTC GGCAAAACCA 
AAACTCAGGT TCTCCTCATC CACAAAGGAA CTTTCAACTG TGGATTTGGT GGTTGAAGCA 
GTGTTCGAAG ACATGAACCT GAAGAAAAAG GTCTTTGCTG AGCTGTCAGC CCTGTGCAAG 
CCAGGAGCCT TTCTGTGCAC CAATACCTCG GCGCTGAACG TGGACGACAT TGCTTCTTCC 
ACAGATCGCC CTCAGCTGGT GATTGGCACC CACTTCTTCT CACCAGCCCA TGTCATGAGG 
TTGCTAGAGG TCATTCCTAG CCGATACTCT TCCCCTACTA CCATCGCCAC GGTTATGAGC 
TTGTCCAAAA AGATCGGAAA GATTGGAGTA GTGGTTGGCA ACTGCTATGG ATTTGTTGGG 
AATCGGATGT TGGCTCCCTA TTACAACCAG GGGTTTTTCT TGTTAGAGGA AGGTAGCAAG 
CCAGAGGATG TAGACGGGGT CTTGGAAGAG TTTGGTTTTA AAATGGGACC CTTCAGAGTG 
TCAGACCTCG CAGGGCTAGA TGTGGGTTGG AAAATTCGCA AGGGGCAAGG CCTTACTGGA 
CCATCATTGC CACCAGGGAC CCCCGTCCGA AAGAGGGGCA ACAGCAGGTA CTCCCCACTT 
GGCGATATGC TCTGTGAAGC TGGGCGGTTT GGTCAGAAGA CAGGTAAAGG CTGGTATCAG 
TATGACAAGC CACTGGGTCG CATCCACAAA CCTGATCCCT GGCTTTCTAC GTTCCTGTCA 
CAATATAGAG AGGTTCACCA CATCGAGCAG CGCACCATCA GCAAGGAGGA GATCCTGGAG 
CGTTGCTTAT ATTCCCTCAT CAATGAGGCG TTCCGCATCT TGGAGGAGGG GATGGCTGCT 
CGCCCAGAGC ACATTGATGT CATCTACTTG CACGGGTACG GGTGGCCAAG GCACAAGGGC 
GGGCCCATGT TCTATGCTGC CTCAGTTGGG TTGCCCACAG TTCTAGAGAA ACTGCAGAAA 
TATTACAGGC AGAACCCTGA CATCCCCCAG CTGGAGCCCA GTGACTACCT CAGAAGGCTG 
GTAGCCCAGG GAAGCCCTCC TCTGAAGGAA TGGCAAAGCT TGGCAGGGCC CCACGGCAGC 
AAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598290.1
CDS	22..2190
Translation	MAEYLRLPHSLAMIRLCNPPVNAVSPTVIREVRNGLQKAGSDHTVKAIVICGANGNFCAGADIHGFSAFTPGLALGSLVDEIQRYQKPVLAAIQGVALGGGLELALGCHYRIANAKARVGLPEVTLGILPGARGTQLLPRVVGVPVALDLITSGKYLSADEALRLGILDAVVKSDPVEEAIKFAQKIIDKPIEPRRIFNKPVPSLPNMDSVFAEAIAKVRKQYPGVLAPETCVRSIQASVKHPYEVGIKEEEKLFMYLRASGQAKALQYAFFAEKSANKWSTPSGASWKTASAQPVSSVGVLGLGTMGRGIAISFARVGISVVAVESDPKQLDAAKKIITFTLEKEASRAHQNGQASAKPKLRFSSSTKELSTVDLVVEAVFEDMNLKKKVFAELSALCKPGAFLCTNTSALNVDDIASSTDRPQLVIGTHFFSPAHVMRLLEVIPSRYSSPTTIATVMSLSKKIGKIGVVVGNCYGFVGNRMLAPYYNQGFFLLEEGSKPEDVDGVLEEFGFKMGPFRVSDLAGLDVGWKIRKGQGLTGPSLPPGTPVRKRGNSRYSPLGDMLCEAGRFGQKTGKGWYQYDKPLGRIHKPDPWLSTFLSQYREVHHIEQRTISKEEILERCLYSLINEAFRILEEGMAARPEHIDVIYLHGYGWPRHKGGPMFYAASVGLPTVLEKLQKYYRQNPDIPQLEPSDYLRRLVAQGSPPLKEWQSLAGPHGSKL

Target ORF information:

RefSeq Version	NM_133606.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase (Ehhadh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133606.1

ORF Insert Sequence:

ATGGCTGAGT ATCTGAGGCT GCCCCACTCC CTGGCGATGA TCCGCCTCTG CAACCCGCCA 
GTCAACGCCG TCAGTCCAAC TGTAATCAGG GAAGTAAGAA ATGGACTCCA GAAAGCTGGA 
TCAGACCACA CGGTTAAAGC CATTGTGATC TGTGGCGCAA ACGGAAACTT CTGTGCAGGT 
GCTGATATCC ATGGCTTTAG TGCTTTTACC CCTGGCCTTG CATTAGGAAG CTTGGTAGAT 
GAAATACAGA GATACCAGAA GCCGGTGTTG GCCGCTATCC AAGGTGTGGC TCTCGGAGGA 
GGACTGGAGC TGGCCTTGGG CTGTCACTAT CGGATTGCCA ATGCAAAGGC TCGTGTCGGC 
TTGCCAGAAG TCACACTGGG GATTCTTCCT GGTGCAAGAG GAACCCAGCT TCTCCCCAGG 
GTCGTTGGAG TTCCTGTTGC TCTTGACCTA ATTACCTCAG GAAAATATCT TTCAGCAGAT 
GAAGCACTCA GGCTTGGAAT TCTGGATGCA GTCGTGAAGT CAGACCCAGT TGAAGAAGCC 
ATCAAATTTG CTCAGAAGAT TATAGATAAA CCCATAGAAC CCCGCAGGAT CTTTAACAAG 
CCAGTTCCAA GCTTGCCCAA CATGGACAGT GTTTTTGCAG AAGCCATCGC CAAGGTACGA 
AAACAGTACC CTGGTGTCCT GGCTCCGGAG ACGTGTGTCC GCTCAATCCA GGCCTCTGTG 
AAGCATCCCT ACGAAGTAGG CATCAAGGAA GAGGAAAAGC TGTTTATGTA CCTCCGGGCA 
TCCGGGCAGG CTAAAGCCCT ACAGTATGCC TTCTTTGCTG AAAAGTCTGC AAATAAGTGG 
TCAACTCCCT CAGGAGCGTC TTGGAAAACA GCCTCTGCTC AACCCGTCTC CTCAGTTGGC 
GTTCTTGGCT TGGGAACGAT GGGCCGAGGC ATCGCCATTT CTTTTGCGAG AGTGGGGATC 
TCTGTGGTTG CTGTGGAGTC AGACCCAAAG CAGCTAGATG CTGCAAAGAA GATAATCACT 
TTCACCTTGG AGAAGGAAGC ATCCAGAGCG CATCAGAACG GCCAAGCTTC GGCAAAACCA 
AAACTCAGGT TCTCCTCATC CACAAAGGAA CTTTCAACTG TGGATTTGGT GGTTGAAGCA 
GTGTTCGAAG ACATGAACCT GAAGAAAAAG GTCTTTGCTG AGCTGTCAGC CCTGTGCAAG 
CCAGGAGCCT TTCTGTGCAC CAATACCTCG GCGCTGAACG TGGACGACAT TGCTTCTTCC 
ACAGATCGCC CTCAGCTGGT GATTGGCACC CACTTCTTCT CACCAGCCCA TGTCATGAGG 
TTGCTAGAGG TCATTCCTAG CCGATACTCT TCCCCTACTA CCATCGCCAC GGTTATGAGC 
TTGTCCAAAA AGATCGGAAA GATTGGAGTA GTGGTTGGCA ACTGCTATGG ATTTGTTGGG 
AATCGGATGT TGGCTCCCTA TTACAACCAG GGGTTTTTCT TGTTAGAGGA AGGTAGCAAG 
CCAGAGGATG TAGACGGGGT CTTGGAAGAG TTTGGTTTTA AAATGGGACC CTTCAGAGTG 
TCAGACCTCG CAGGGCTAGA TGTGGGTTGG AAAATTCGCA AGGGGCAAGG CCTTACTGGA 
CCATCATTGC CACCAGGGAC CCCCGTCCGA AAGAGGGGCA ACAGCAGGTA CTCCCCACTT 
GGCGATATGC TCTGTGAAGC TGGGCGGTTT GGTCAGAAGA CAGGTAAAGG CTGGTATCAG 
TATGACAAGC CACTGGGTCG CATCCACAAA CCTGATCCCT GGCTTTCTAC GTTCCTGTCA 
CAATATAGAG AGGTTCACCA CATCGAGCAG CGCACCATCA GCAAGGAGGA GATCCTGGAG 
CGTTGCTTAT ATTCCCTCAT CAATGAGGCG TTCCGCATCT TGGAGGAGGG GATGGCTGCT 
CGCCCAGAGC ACATTGATGT CATCTACTTG CACGGGTACG GGTGGCCAAG GCACAAGGGC 
GGGCCCATGT TCTATGCTGC CTCAGTTGGG TTGCCCACAG TTCTAGAGAA ACTGCAGAAA 
TATTACAGGC AGAACCCTGA CATCCCCCAG CTGGAGCCCA GTGACTACCT CAGAAGGCTG 
GTAGCCCAGG GAAGCCCTCC TCTGAAGGAA TGGCAAAGCT TGGCAGGGCC CCACGGCAGC 
AAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The isomerase and hydratase reaction mechanism of the crotonase active site of the multifunctional enzyme (type-1), as deduced from structures of complexes with 3S-hydroxy-acyl-CoA.
The FEBS journal280(13)3160-75(2013 Jul)
Kasaragod P,Schmitz W,Hiltunen JK,Wierenga RK

Crystal structure of liganded rat peroxisomal multifunctional enzyme type 1: a flexible molecule with two interconnected active sites.
The Journal of biological chemistry285(31)24089-98(2010 Jul)
Kasaragod P,Venkatesan R,Kiema TR,Hiltunen JK,Wierenga RK

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Journal of the American Chemical Society132(11)3973-9(2010 Mar)
Ghosh D,Berg JM

Regulation of cellular metabolism by protein lysine acetylation.
Science (New York, N.Y.)327(5968)1000-4(2010 Feb)
Zhao S,Xu W,Jiang W,Yu W,Lin Y,Zhang T,Yao J,Zhou L,Zeng Y,Li H,Li Y,Shi J,An W,Hancock SM,He F,Qin L,Chin J,Yang P,Chen X,Lei Q,Xiong Y,Guan KL

Beta-oxidation in hepatocyte cultures from mice with peroxisomal gene knockouts.
Biochemical and biophysical research communications357(3)718-23(2007 Jun)
Dirkx R,Meyhi E,Asselberghs S,Reddy J,Baes M,Van Veldhoven PP

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Ehhadh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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