Cblb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cblb
Entrez Gene ID	171136
Full Name	Cbl proto-oncogene B
General protein information	Preferred Names Cbl proto-oncogene B Names E3 ubiquitin-protein ligase CBL-B Cas-Br-M (murine) ecotropic retroviral transforming sequence b Cas-Br-M (murine) ectropic retroviral transforming sequence b Casitas B-lineage lymphoma b Cbl proto-oncogene B, E3 ubiquitin protein ligase Cbl proto-oncogene, E3 ubiquitin protein ligase B RING-type E3 ubiquitin transferase CBL-B SH3-binding protein CBL-B casitas B-lineage lymphoma proto-oncogene b signal transduction protein CBL-B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q21
Summary	ubiquitin-protein ligase; may regulate autoimmunity; implicated as a susceptibility gene for type I diabetes (IDDM) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006248258.3	XP_006248320.1	E3 ubiquitin-protein ligase CBL-B isoform X1
NM_133601.1	NP_598285.1	E3 ubiquitin-protein ligase CBL-B
XM_008768626.2	XP_008766848.1	E3 ubiquitin-protein ligase CBL-B isoform X1
XM_006248259.3	XP_006248321.1	E3 ubiquitin-protein ligase CBL-B isoform X1
XM_006248260.3	XP_006248322.1	E3 ubiquitin-protein ligase CBL-B isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cblb	NP_598285.1
Macaca mulatta (Rhesus monkey)	LOC703879	XP_002808356.1
Danio rerio (zebrafish)	cblb	XP_001921961.4
Xenopus tropicalis (tropical clawed frog)	cblb	NP_001006802.1
Canis lupus familiaris (dog)	CBLB	XP_005639497.1
Bos taurus (cattle)	CBLB	NP_001192852.1
Gallus gallus (chicken)	CBLB	XP_416621.3
Homo sapiens (human)	CBLB	NP_733762.2
Pan troglodytes (chimpanzee)	CBLB	XP_003309960.1
Mus musculus (house mouse)	Cblb	NP_001028410.1

Gene Ontology
Bibliography

Related articles in PubMed

Increased expression of the Cbl family of E3 ubiquitin ligases decreases Interleukin-2 production in a rat model of peripheral neuropathy.
Jeong JS, Kim HY, Shin BS, Lee AR, Yoon JH, Hahm TS, Lee JE
BMC anesthesiology18(1)87(2018 07)

Reactive oxygen species upregulate expression of muscle atrophy-associated ubiquitin ligase Cbl-b in rat L6 skeletal muscle cells.
Uchida T, Sakashita Y, Kitahata K, Yamashita Y, Tomida C, Kimori Y, Komatsu A, Hirasaka K, Ohno A, Nakao R, Higashitani A, Higashibata A, Ishioka N, Shimazu T, Kobayashi T, Okumura Y, Choi I, Oarada M, Mills EM, Teshima-Kondo S, Takeda S, Tanaka E, Tanaka K, Sokabe M, Nikawa T
American journal of physiology. Cell physiology314(6)C721-C731(2018 06)

IFT20 modulates ciliary PDGFRα signaling by regulating the stability of Cbl E3 ubiquitin ligases.
Schmid FM, Schou KB, Vilhelm MJ, Holm MS, Breslin L, Farinelli P, Larsen LA, Andersen JS, Pedersen LB, Christensen ST
The Journal of cell biology217(1)151-161(2018 01)

Cbl-b promotes chemotherapy-induced apoptosis in rat basophilic leukemia cells by suppressing PI3K/Akt activation and enhancing MEK/ERK activation.
Qu X, Li Y, Liu J, Xu L, Zhang Y, Hu X, Hou K, Liu Y
Molecular and cellular biochemistry340(1-2)107-14(2010 Jul)

Ubiquitin ligase Cbl-b is a negative regulator for insulin-like growth factor 1 signaling during muscle atrophy caused by unloading.
Nakao R, Hirasaka K, Goto J, Ishidoh K, Yamada C, Ohno A, Okumura Y, Nonaka I, Yasutomo K, Baldwin KM, Kominami E, Higashibata A, Nagano K, Tanaka K, Yasui N, Mills EM, Takeda S, Nikawa T
Molecular and cellular biology29(17)4798-811(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cblb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cblb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01045	NM_133601.1 Latest version!	Rattus norvegicus Cbl proto-oncogene B (Cblb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa34235	XM_006248258.3 Latest version!	Rattus norvegicus Cbl proto-oncogene B (Cblb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa34235	XM_008768626.2 Latest version!	Rattus norvegicus Cbl proto-oncogene B (Cblb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa34235	XM_006248259.3 Latest version!	Rattus norvegicus Cbl proto-oncogene B (Cblb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa34236	XM_006248260.3 Latest version!	Rattus norvegicus Cbl proto-oncogene B (Cblb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cblb ( NM_133601.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_598285.1 Rattus norvegicus Cbl proto-oncogene B (Cblb), mRNA.

Price & Availability↑

CloneID	ORa01045
Clone ID Related Accession (Same CDS sequence)	NM_133601.1
Accession Version	NM_133601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568908800000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase CBL-B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB071283.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB071283.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCAAATT CTATGAATGG CAGAAATCCT GGTGGTCGAG GAGGAAACCC CCGCAAAGGT 
CGAATTTTGG GGATTATTGA TGCCATTCAG GATGCAGTTG GACCCCCAAA GCAAGCTGCA 
GCTGACCGCA GGACAGTGGA GAAGACTTGG AAACTCATGG ACAAAGTGGT AAGACTGTGC 
CAAAATCCGA AACTTCAGTT GAAAAACAGC CCACCATATA TCCTCGACAT TTTACCTGAT 
ACGTATCAGC ATTTGCGGCT TATATTGAGT AAGTATGACG ACAACCAGAA GCTGGCTCAA 
CTGAGCGAGA ATGAGTACTT TAAAATCTAC ATCGACAGTC TCATGAAGAA GTCAAAGCGA 
GCGATCCGGC TCTTCAAAGA AGGCAAGGAG AGGATGTACG AGGAGCAGTC GCAGGACAGA 
CGGAATCTCA CAAAGCTGTC CCTTATCTTC AGTCACATGC TGGCAGAAAT CAAGGCGATC 
TTTCCCAATG GCCAGTTCCA GGGAGATAAC TTCCGGATCA CCAAAGCAGA TGCTGCCGAA 
TTCTGGAGGA AGTTTTTTGG AGACAAAACT ATCGTACCAT GGAAAGTCTT CAGACAGTGC 
CTGCATGAGG TCCATCAGAT CAGCTCTGGC CTGGAGGCCA TGGCTCTGAA GTCAACCATT 
GACTTAACTT GTAATGATTA CATCTCCGTG TTTGAATTTG ATATTTTTAC CAGGCTATTT 
CAGCCCTGGG GCTCTATTTT ACGGAATTGG AACTTCTTAG CTGTGACACA CCCGGGGTAC 
ATGGCATTTC TCACATATGA TGAAGTTAAA GCTCGACTAC AGAAATACAG CACCAAGCCT 
GGAAGCTACA TTTTCCGGTT AAGCTGCACT CGGCTGGGAC AATGGGCCAT TGGCTATGTG 
ACTGGGGACG GCAATATCCT ACAGACCATA CCTCATAACA AGCCCCTGTT CCAAGCCCTG 
ATTGATGGTA GCAGGGAAGG CTTTTACCTT TATCCAGATG GACGAAGCTA TAACCCTGAT 
TTAACCGGAT TATGTGAACC TACACCTCAT GATCATATAA AAGTTACACA GGAGCAATAT 
GAACTGTATT GTGAAATGGG CTCCACTTTT CAGCTGTGCA AGATCTGTGC AGAGAATGAC 
AAAGATGTCA AGATCGAGCC TTGTGGGCAT CTCATGTGCA CTTCGTGCCT TACCGCGTGG 
CAGGAGTCTG ATGGCCAAGG CTGCCCCTTC TGTCGCTGTG AGATAAAAGG AACCGAACCT 
ATCATCGTGG ATCCCTTTGA CCCCAGAGAC GAAGGCTCCA GGTGCTGCAG CATCATCGAC 
CCTTTCAGCA TCCCCATGCT CGACTTGGAT GATGACGATG ATCGAGAGGA GTCTCTGATG 
ATGAACCGGC TGGCGAGTGT TCGCAAGTGC ACAGACAGGC AGAACTCGCC AGTCACATCG 
CCAGGATCCT CACCCCTTGC CCAGAGAAGA AAGCCTCAGC CAGACCCTCT CCAGATCCCC 
CACCTCAGCC TGCCACCAGT GCCTCCCCGC CTGGACCTCA TTCAGAAAGG CATCGTGCGC 
TCTCCCTGTG GCAGCCCCAC GGGCTCCCCG AAGTCTTCTC CATGCATGGT TAGAAAACAA 
GACAAACCAC TCCCAGCACC CCCTCCTCCC TTGCGAGATC CTCCGCCTCC ACCAGAGCGG 
CCTCCGCCAA TCCCGCCTGA CAGTAGACTG AGCAGACACT TCCACCACGG AGAGAGTGTG 
CCTTCCAGGG ACCAGCCAAT GCCTCTTGAA GCCTGGTGCC CTCGGGATGC CTTCGGGACT 
AATCAGGTGA TGGGATGTCG CATCCTAGGG GATGGCTCTC CAAAGCCTGG CGTCACAGCA 
AACTCCAACT TAAATGGACG TCACAGTCGA ATGGGCTCTG ACCAGGTTCT TATGAGGAAA 
CACAGACGCC ACGATTTGCC TTCAGAAGGC GCCAAGGTCT TTTCCAATGG ACACCTTGCC 
CCTGAAGAAT ACGACGTTCC TCCTCGGCTT TCCCCTCCTC CTCCAGTCAC TGCCCTTCTC 
CCTAGCATAA AGTGTACTGG TCCAATAGCA AATTGTCTCT CCGAGAAAAC AAGAGACACA 
GTAGAAGAAG ATGATGATGA ATACAAGATT CCTTCATCCC ATCCTGTTTC CCTGAATTCA 
CAACCATCTC ATTGTCATAA TGTCAAACCT CCTGTTCGGT CTTGTGATAA TGGTCACTGT 
ATACTGAATG GAACTCATGG TACGCCTTCA GAGATGAAGA AATCAAACAT CCCAGATTTA 
GGCATCTATT TGAAGGGTGA AGATGCTTTT GATGCCCTCC CCCCATCCCT TCCTCCTCCC 
CCACCTCCTG CAAGACATAG TCTCATCGAG CATTCAAAAC CTCCAGGCTC CAGTAGCCGG 
CCTTCCTCAG GACAGGACCT TTTCCTTCTT CCTTCAGATC CCTTTTTTGA CCCAGCAAGT 
GGCCAAGTTC CATTGCCTCC GGCCAGGAGA GCACCAGGAG ATGGTGTCAA ATCCAACAGA 
GCCTCCCAGG ACTATGACCA GCTCCCTTCA TCTTCCGATG GTTCGCAAGC ACCAGCTAGA 
CCCCCCAAAC CACGACCCCG AAGGACTGCA CCAGAAATTC ATCACAGAAA GCCCCATGGG 
CCCGAGGCGG CACTGGAAAA TGTGGATGCG AAAATTGCAA AACTCATGGG AGAGGGGTAT 
GCCTTTGAAG AGGTGAAGAG AGCCTTAGAG ATCGCCCAGA ATAACCTGGA AGTGGCCAGG 
AGCATACTTC GAGAATTCGC CTTCCCTCCT CCCGTCTCGC CACGTCTCAA TCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598285.1
CDS	45..2861
Translation	MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNSPPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDRRNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKPGSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPHDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCEIKGTEPIIVDPFDPRDEGSRCCSIIDPFSIPMLDLDDDDDREESLMMNRLASVRKCTDRQNSPVTSPGSSPLAQRRKPQPDPLQIPHLSLPPVPPRLDLIQKGIVRSPCGSPTGSPKSSPCMVRKQDKPLPAPPPPLRDPPPPPERPPPIPPDSRLSRHFHHGESVPSRDQPMPLEAWCPRDAFGTNQVMGCRILGDGSPKPGVTANSNLNGRHSRMGSDQVLMRKHRRHDLPSEGAKVFSNGHLAPEEYDVPPRLSPPPPVTALLPSIKCTGPIANCLSEKTRDTVEEDDDEYKIPSSHPVSLNSQPSHCHNVKPPVRSCDNGHCILNGTHGTPSEMKKSNIPDLGIYLKGEDAFDALPPSLPPPPPPARHSLIEHSKPPGSSSRPSSGQDLFLLPSDPFFDPASGQVPLPPARRAPGDGVKSNRASQDYDQLPSSSDGSQAPARPPKPRPRRTAPEIHHRKPHGPEAALENVDAKIAKLMGEGYAFEEVKRALEIAQNNLEVARSILREFAFPPPVSPRLNL

Target ORF information:

RefSeq Version	NM_133601.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Cbl proto-oncogene B (Cblb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133601.1

ORF Insert Sequence:

ATGGCAAATT CTATGAATGG CAGAAATCCT GGTGGTCGAG GAGGAAACCC CCGCAAAGGT 
CGAATTTTGG GGATTATTGA TGCCATTCAG GATGCAGTTG GACCCCCAAA GCAAGCTGCA 
GCTGACCGCA GGACAGTGGA GAAGACTTGG AAACTCATGG ACAAAGTGGT AAGACTGTGC 
CAAAATCCGA AACTTCAGTT GAAAAACAGC CCACCATATA TCCTCGACAT TTTACCTGAT 
ACGTATCAGC ATTTGCGGCT TATATTGAGT AAGTATGACG ACAACCAGAA GCTGGCTCAA 
CTGAGCGAGA ATGAGTACTT TAAAATCTAC ATCGACAGTC TCATGAAGAA GTCAAAGCGA 
GCGATCCGGC TCTTCAAAGA AGGCAAGGAG AGGATGTACG AGGAGCAGTC GCAGGACAGA 
CGGAATCTCA CAAAGCTGTC CCTTATCTTC AGTCACATGC TGGCAGAAAT CAAGGCGATC 
TTTCCCAATG GCCAGTTCCA GGGAGATAAC TTCCGGATCA CCAAAGCAGA TGCTGCCGAA 
TTCTGGAGGA AGTTTTTTGG AGACAAAACT ATCGTACCAT GGAAAGTCTT CAGACAGTGC 
CTGCATGAGG TCCATCAGAT CAGCTCTGGC CTGGAGGCCA TGGCTCTGAA GTCAACCATT 
GACTTAACTT GTAATGATTA CATCTCCGTG TTTGAATTTG ATATTTTTAC CAGGCTATTT 
CAGCCCTGGG GCTCTATTTT ACGGAATTGG AACTTCTTAG CTGTGACACA CCCGGGGTAC 
ATGGCATTTC TCACATATGA TGAAGTTAAA GCTCGACTAC AGAAATACAG CACCAAGCCT 
GGAAGCTACA TTTTCCGGTT AAGCTGCACT CGGCTGGGAC AATGGGCCAT TGGCTATGTG 
ACTGGGGACG GCAATATCCT ACAGACCATA CCTCATAACA AGCCCCTGTT CCAAGCCCTG 
ATTGATGGTA GCAGGGAAGG CTTTTACCTT TATCCAGATG GACGAAGCTA TAACCCTGAT 
TTAACCGGAT TATGTGAACC TACACCTCAT GATCATATAA AAGTTACACA GGAGCAATAT 
GAACTGTATT GTGAAATGGG CTCCACTTTT CAGCTGTGCA AGATCTGTGC AGAGAATGAC 
AAAGATGTCA AGATCGAGCC TTGTGGGCAT CTCATGTGCA CTTCGTGCCT TACCGCGTGG 
CAGGAGTCTG ATGGCCAAGG CTGCCCCTTC TGTCGCTGTG AGATAAAAGG AACCGAACCT 
ATCATCGTGG ATCCCTTTGA CCCCAGAGAC GAAGGCTCCA GGTGCTGCAG CATCATCGAC 
CCTTTCAGCA TCCCCATGCT CGACTTGGAT GATGACGATG ATCGAGAGGA GTCTCTGATG 
ATGAACCGGC TGGCGAGTGT TCGCAAGTGC ACAGACAGGC AGAACTCGCC AGTCACATCG 
CCAGGATCCT CACCCCTTGC CCAGAGAAGA AAGCCTCAGC CAGACCCTCT CCAGATCCCC 
CACCTCAGCC TGCCACCAGT GCCTCCCCGC CTGGACCTCA TTCAGAAAGG CATCGTGCGC 
TCTCCCTGTG GCAGCCCCAC GGGCTCCCCG AAGTCTTCTC CATGCATGGT TAGAAAACAA 
GACAAACCAC TCCCAGCACC CCCTCCTCCC TTGCGAGATC CTCCGCCTCC ACCAGAGCGG 
CCTCCGCCAA TCCCGCCTGA CAGTAGACTG AGCAGACACT TCCACCACGG AGAGAGTGTG 
CCTTCCAGGG ACCAGCCAAT GCCTCTTGAA GCCTGGTGCC CTCGGGATGC CTTCGGGACT 
AATCAGGTGA TGGGATGTCG CATCCTAGGG GATGGCTCTC CAAAGCCTGG CGTCACAGCA 
AACTCCAACT TAAATGGACG TCACAGTCGA ATGGGCTCTG ACCAGGTTCT TATGAGGAAA 
CACAGACGCC ACGATTTGCC TTCAGAAGGC GCCAAGGTCT TTTCCAATGG ACACCTTGCC 
CCTGAAGAAT ACGACGTTCC TCCTCGGCTT TCCCCTCCTC CTCCAGTCAC TGCCCTTCTC 
CCTAGCATAA AGTGTACTGG TCCAATAGCA AATTGTCTCT CCGAGAAAAC AAGAGACACA 
GTAGAAGAAG ATGATGATGA ATACAAGATT CCTTCATCCC ATCCTGTTTC CCTGAATTCA 
CAACCATCTC ATTGTCATAA TGTCAAACCT CCTGTTCGGT CTTGTGATAA TGGTCACTGT 
ATACTGAATG GAACTCATGG TACGCCTTCA GAGATGAAGA AATCAAACAT CCCAGATTTA 
GGCATCTATT TGAAGGGTGA AGATGCTTTT GATGCCCTCC CCCCATCCCT TCCTCCTCCC 
CCACCTCCTG CAAGACATAG TCTCATCGAG CATTCAAAAC CTCCAGGCTC CAGTAGCCGG 
CCTTCCTCAG GACAGGACCT TTTCCTTCTT CCTTCAGATC CCTTTTTTGA CCCAGCAAGT 
GGCCAAGTTC CATTGCCTCC GGCCAGGAGA GCACCAGGAG ATGGTGTCAA ATCCAACAGA 
GCCTCCCAGG ACTATGACCA GCTCCCTTCA TCTTCCGATG GTTCGCAAGC ACCAGCTAGA 
CCCCCCAAAC CACGACCCCG AAGGACTGCA CCAGAAATTC ATCACAGAAA GCCCCATGGG 
CCCGAGGCGG CACTGGAAAA TGTGGATGCG AAAATTGCAA AACTCATGGG AGAGGGGTAT 
GCCTTTGAAG AGGTGAAGAG AGCCTTAGAG ATCGCCCAGA ATAACCTGGA AGTGGCCAGG 
AGCATACTTC GAGAATTCGC CTTCCCTCCT CCCGTCTCGC CACGTCTCAA TCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cblb ( XM_006248258.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248320.1 Rattus norvegicus Cbl proto-oncogene B (Cblb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa34235
Clone ID Related Accession (Same CDS sequence)	XM_006248258.3 , XM_008768626.2 , XM_006248259.3
Accession Version	XM_006248258.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase CBL-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248258.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATT CTATGAATGG CAGAAATCCT GGTGGTCGAG GAGGAAACCC CCGCAAAGGT 
CGAATTTTGG GGATTATTGA TGCCATTCAG GATGCAGTTG GACCCCCAAA GCAAGCTGCA 
GCTGACCGCA GGACAGTGGA GAAGACTTGG AAACTCATGG ACAAAGTGGT AAGACTGTGC 
CAAAATCCGA AACTTCAGTT GAAAAACAGC CCACCATATA TCCTCGACAT TTTACCTGAT 
ACGTATCAGC ATTTGCGGCT TATATTGAGT AAGTATGACG ACAACCAGAA GCTGGCTCAA 
CTGAGCGAGA ATGAGTACTT TAAAATCTAC ATCGACAGTC TCATGAAGAA GTCAAAGCGA 
GCGATCCGGC TCTTCAAAGA AGGCAAGGAG AGGATGTACG AGGAGCAGTC GCAGGACAGA 
CGGAATCTCA CAAAGCTGTC CCTTATCTTC AGTCACATGC TGGCAGAAAT CAAGGCGATC 
TTTCCCAATG GCCAGTTCCA GGGAGATAAC TTCCGGATCA CCAAAGCAGA TGCTGCCGAA 
TTCTGGAGGA AGTTTTTTGG AGACAAAACT ATCGTACCAT GGAAAGTCTT CAGACAGTGC 
CTGCATGAGG TCCATCAGAT CAGCTCTGGC CTGGAGGCCA TGGCTCTGAA GTCAACCATT 
GACTTAACTT GTAATGATTA CATCTCCGTG TTTGAATTTG ATATTTTTAC CAGGCTATTT 
CAGCCCTGGG GCTCTATTTT ACGGAATTGG AACTTCTTAG CTGTGACACA CCCGGGGTAC 
ATGGCATTTC TCACATATGA TGAAGTTAAA GCTCGACTAC AGAAATACAG CACCAAGCCT 
GGAAGCTACA TTTTCCGGTT AAGCTGCACT CGGCTGGGAC AATGGGCCAT TGGCTATGTG 
ACTGGGGACG GCAATATCCT ACAGACCATA CCTCATAATA AGCCCCTGTT CCAAGCCCTG 
ATTGATGGTA GCAGGGAAGG CTTTTACCTT TATCCAGATG GACGAAGCTA TAACCCTGAT 
TTAACCGGAT TATGTGAACC TACACCTCAT GATCATATAA AAGTTACACA GGAGCAATAT 
GAACTGTATT GTGAAATGGG CTCCACTTTT CAGCTGTGCA AGATCTGTGC AGAGAATGAC 
AAAGATGTCA AGATCGAGCC TTGCGGGCAT CTCATGTGCA CTTCGTGCCT TACCGCGTGG 
CAGGAGTCTG ATGGCCAAGG CTGCCCCTTC TGTCGCTGTG AGATAAAAGG AACCGAACCT 
ATCATCGTGG ATCCCTTTGA CCCCAGAGAC GAAGGCTCCA GGTGCTGCAG CATCATCGAC 
CCTTTCAGCA TCCCCATGCT CGACTTGGAT GATGACGATG ATCGAGAGGA GTCTCTGATG 
ATGAACCGGC TGGCGAGTGT TCGCAAGTGC ACAGACAGGC AGAACTCGCC AGTCACATCG 
CCAGGATCCT CACCCCTTGC CCAGAGAAGA AAGCCTCAGC CAGACCCTCT CCAGATCCCC 
CACCTCAGCC TGCCACCAGT GCCTCCCCGC CTGGACCTCA TTCAGAAAGG CATCGTGCGC 
TCTCCCTGTG GCAGCCCCAC GGGCTCCCCG AAGTCTTCTC CATGCATGGT TAGAAAACAA 
GACAAACCAC TCCCAGCACC CCCTCCTCCC TTGCGAGATC CTCCGCCTCC ACCAGAGCGG 
CCTCCGCCAA TCCCGCCTGA CAGTAGACTG AGCAGACACT TCCACCACGG AGAGGGTGTG 
CCTTCCAGGG ACCAGCCAAT GCCTCTTGAA GCCTGGTGCC CTCGGGATGC CTTCGGGACT 
AATCAGGTGA TGGGATGTCG CATCCTAGGG GATGGCTCTC CAAAGCCTGG CGTCACAGCA 
AACTCCAACT TAAATGGACG TCACAGTCGA ATGGGCTCTG ACCAGGTTCT TATGAGGAAA 
CACAGACGCC ACGATTTGCC TTCAGAAGGC GCCAAGGTCT TTTCCAATGG ACACCTTGCC 
CCTGAAGAAT ACGACGTTCC TCCTCGGCTT TCCCCTCCTC CTCCAGTCAC TGCCCTTCTC 
CCTAGCATAA AGTGTACTGG TCCAATAGCA AATTGTCTCT CCGAGAAAAC AAGAGACACA 
GTAGAAGAAG ATGATGATGA ATACAAGATT CCTTCATCCC ATCCTGTTTC CCTGAATTCA 
CAACCATCTC ATTGTCATAA TGTCAAACCT CCTGTTCGGT CTTGTGATAA TGGTCACTGT 
ATACTGAATG GAACTCATGG TACGCCTTCA GAGATGAAGA AATCAAACAT CCCAGATTTA 
GGCATCTATT TGAAGGGAGA TGTTTCTGAT TCAGCCTCTG ATCCAGTACC ATTACCACCT 
GCCAGGCCTC CACCCCGGGA CAACCTAAAG CATGGTTCTT CAGTCAACAG GACGCCCTCT 
GATTATGATC TTCTCATCCC TCCATTAGGT GAAGATGCTT TTGATGCCCT CCCCCCATCC 
CTTCCTCCTC CTCCACCTCC TGCAAGACAT AGTCTCATCG AGCATTCAAA ACCTCCAGGC 
TCCAGTAGCC GGCCTTCCTC AGGACAGGAC CTTTTCCTTC TTCCTTCAGA TCCCTTTTTT 
GACCCAGCAA GTGGCCAAGT TCCATTGCCT CCGGCCAGGA GAGCACCAGG AGATGGTGTC 
AAATCCAACA GAGCCTCCCA GGACTATGAC CAGCTCCCTT CATCTTCCGA TGGTTCGCAA 
GCACCAGCTA GACCCCCCAA ACCACGACCC CGAAGGACTG CACCAGAAAT TCATCACAGA 
AAGCCCCATG GGCCCGAGGC GGCACTGGAA AATGTGGATG CGAAAATTGC AAAACTCATG 
GGAGAGGGGT ATGCCTTTGA AGAGGTGAAG AGAGCCTTAG AGATCGCCCA GAATAACCTG 
GAAGTGGCCA GGAGCATACT TCGAGAATTC GCCTTCCCTC CTCCCGTCTC GCCACGTCTC 
AATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248320.1
CDS	103..3051
Translation	MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNSPPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDRRNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKPGSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPHDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCEIKGTEPIIVDPFDPRDEGSRCCSIIDPFSIPMLDLDDDDDREESLMMNRLASVRKCTDRQNSPVTSPGSSPLAQRRKPQPDPLQIPHLSLPPVPPRLDLIQKGIVRSPCGSPTGSPKSSPCMVRKQDKPLPAPPPPLRDPPPPPERPPPIPPDSRLSRHFHHGEGVPSRDQPMPLEAWCPRDAFGTNQVMGCRILGDGSPKPGVTANSNLNGRHSRMGSDQVLMRKHRRHDLPSEGAKVFSNGHLAPEEYDVPPRLSPPPPVTALLPSIKCTGPIANCLSEKTRDTVEEDDDEYKIPSSHPVSLNSQPSHCHNVKPPVRSCDNGHCILNGTHGTPSEMKKSNIPDLGIYLKGDVSDSASDPVPLPPARPPPRDNLKHGSSVNRTPSDYDLLIPPLGEDAFDALPPSLPPPPPPARHSLIEHSKPPGSSSRPSSGQDLFLLPSDPFFDPASGQVPLPPARRAPGDGVKSNRASQDYDQLPSSSDGSQAPARPPKPRPRRTAPEIHHRKPHGPEAALENVDAKIAKLMGEGYAFEEVKRALEIAQNNLEVARSILREFAFPPPVSPRLNL

Target ORF information:

RefSeq Version	XM_006248258.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Cbl proto-oncogene B (Cblb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248258.3

ORF Insert Sequence:

ATGGCAAATT CTATGAATGG CAGAAATCCT GGTGGTCGAG GAGGAAACCC CCGCAAAGGT 
CGAATTTTGG GGATTATTGA TGCCATTCAG GATGCAGTTG GACCCCCAAA GCAAGCTGCA 
GCTGACCGCA GGACAGTGGA GAAGACTTGG AAACTCATGG ACAAAGTGGT AAGACTGTGC 
CAAAATCCGA AACTTCAGTT GAAAAACAGC CCACCATATA TCCTCGACAT TTTACCTGAT 
ACGTATCAGC ATTTGCGGCT TATATTGAGT AAGTATGACG ACAACCAGAA GCTGGCTCAA 
CTGAGCGAGA ATGAGTACTT TAAAATCTAC ATCGACAGTC TCATGAAGAA GTCAAAGCGA 
GCGATCCGGC TCTTCAAAGA AGGCAAGGAG AGGATGTACG AGGAGCAGTC GCAGGACAGA 
CGGAATCTCA CAAAGCTGTC CCTTATCTTC AGTCACATGC TGGCAGAAAT CAAGGCGATC 
TTTCCCAATG GCCAGTTCCA GGGAGATAAC TTCCGGATCA CCAAAGCAGA TGCTGCCGAA 
TTCTGGAGGA AGTTTTTTGG AGACAAAACT ATCGTACCAT GGAAAGTCTT CAGACAGTGC 
CTGCATGAGG TCCATCAGAT CAGCTCTGGC CTGGAGGCCA TGGCTCTGAA GTCAACCATT 
GACTTAACTT GTAATGATTA CATCTCCGTG TTTGAATTTG ATATTTTTAC CAGGCTATTT 
CAGCCCTGGG GCTCTATTTT ACGGAATTGG AACTTCTTAG CTGTGACACA CCCGGGGTAC 
ATGGCATTTC TCACATATGA TGAAGTTAAA GCTCGACTAC AGAAATACAG CACCAAGCCT 
GGAAGCTACA TTTTCCGGTT AAGCTGCACT CGGCTGGGAC AATGGGCCAT TGGCTATGTG 
ACTGGGGACG GCAATATCCT ACAGACCATA CCTCATAATA AGCCCCTGTT CCAAGCCCTG 
ATTGATGGTA GCAGGGAAGG CTTTTACCTT TATCCAGATG GACGAAGCTA TAACCCTGAT 
TTAACCGGAT TATGTGAACC TACACCTCAT GATCATATAA AAGTTACACA GGAGCAATAT 
GAACTGTATT GTGAAATGGG CTCCACTTTT CAGCTGTGCA AGATCTGTGC AGAGAATGAC 
AAAGATGTCA AGATCGAGCC TTGCGGGCAT CTCATGTGCA CTTCGTGCCT TACCGCGTGG 
CAGGAGTCTG ATGGCCAAGG CTGCCCCTTC TGTCGCTGTG AGATAAAAGG AACCGAACCT 
ATCATCGTGG ATCCCTTTGA CCCCAGAGAC GAAGGCTCCA GGTGCTGCAG CATCATCGAC 
CCTTTCAGCA TCCCCATGCT CGACTTGGAT GATGACGATG ATCGAGAGGA GTCTCTGATG 
ATGAACCGGC TGGCGAGTGT TCGCAAGTGC ACAGACAGGC AGAACTCGCC AGTCACATCG 
CCAGGATCCT CACCCCTTGC CCAGAGAAGA AAGCCTCAGC CAGACCCTCT CCAGATCCCC 
CACCTCAGCC TGCCACCAGT GCCTCCCCGC CTGGACCTCA TTCAGAAAGG CATCGTGCGC 
TCTCCCTGTG GCAGCCCCAC GGGCTCCCCG AAGTCTTCTC CATGCATGGT TAGAAAACAA 
GACAAACCAC TCCCAGCACC CCCTCCTCCC TTGCGAGATC CTCCGCCTCC ACCAGAGCGG 
CCTCCGCCAA TCCCGCCTGA CAGTAGACTG AGCAGACACT TCCACCACGG AGAGGGTGTG 
CCTTCCAGGG ACCAGCCAAT GCCTCTTGAA GCCTGGTGCC CTCGGGATGC CTTCGGGACT 
AATCAGGTGA TGGGATGTCG CATCCTAGGG GATGGCTCTC CAAAGCCTGG CGTCACAGCA 
AACTCCAACT TAAATGGACG TCACAGTCGA ATGGGCTCTG ACCAGGTTCT TATGAGGAAA 
CACAGACGCC ACGATTTGCC TTCAGAAGGC GCCAAGGTCT TTTCCAATGG ACACCTTGCC 
CCTGAAGAAT ACGACGTTCC TCCTCGGCTT TCCCCTCCTC CTCCAGTCAC TGCCCTTCTC 
CCTAGCATAA AGTGTACTGG TCCAATAGCA AATTGTCTCT CCGAGAAAAC AAGAGACACA 
GTAGAAGAAG ATGATGATGA ATACAAGATT CCTTCATCCC ATCCTGTTTC CCTGAATTCA 
CAACCATCTC ATTGTCATAA TGTCAAACCT CCTGTTCGGT CTTGTGATAA TGGTCACTGT 
ATACTGAATG GAACTCATGG TACGCCTTCA GAGATGAAGA AATCAAACAT CCCAGATTTA 
GGCATCTATT TGAAGGGAGA TGTTTCTGAT TCAGCCTCTG ATCCAGTACC ATTACCACCT 
GCCAGGCCTC CACCCCGGGA CAACCTAAAG CATGGTTCTT CAGTCAACAG GACGCCCTCT 
GATTATGATC TTCTCATCCC TCCATTAGGT GAAGATGCTT TTGATGCCCT CCCCCCATCC 
CTTCCTCCTC CTCCACCTCC TGCAAGACAT AGTCTCATCG AGCATTCAAA ACCTCCAGGC 
TCCAGTAGCC GGCCTTCCTC AGGACAGGAC CTTTTCCTTC TTCCTTCAGA TCCCTTTTTT 
GACCCAGCAA GTGGCCAAGT TCCATTGCCT CCGGCCAGGA GAGCACCAGG AGATGGTGTC 
AAATCCAACA GAGCCTCCCA GGACTATGAC CAGCTCCCTT CATCTTCCGA TGGTTCGCAA 
GCACCAGCTA GACCCCCCAA ACCACGACCC CGAAGGACTG CACCAGAAAT TCATCACAGA 
AAGCCCCATG GGCCCGAGGC GGCACTGGAA AATGTGGATG CGAAAATTGC AAAACTCATG 
GGAGAGGGGT ATGCCTTTGA AGAGGTGAAG AGAGCCTTAG AGATCGCCCA GAATAACCTG 
GAAGTGGCCA GGAGCATACT TCGAGAATTC GCCTTCCCTC CTCCCGTCTC GCCACGTCTC 
AATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cblb ( XM_008768626.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766848.1 Rattus norvegicus Cbl proto-oncogene B (Cblb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa34235
Clone ID Related Accession (Same CDS sequence)	XM_006248258.3 , XM_008768626.2 , XM_006248259.3
Accession Version	XM_008768626.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase CBL-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768626.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATT CTATGAATGG CAGAAATCCT GGTGGTCGAG GAGGAAACCC CCGCAAAGGT 
CGAATTTTGG GGATTATTGA TGCCATTCAG GATGCAGTTG GACCCCCAAA GCAAGCTGCA 
GCTGACCGCA GGACAGTGGA GAAGACTTGG AAACTCATGG ACAAAGTGGT AAGACTGTGC 
CAAAATCCGA AACTTCAGTT GAAAAACAGC CCACCATATA TCCTCGACAT TTTACCTGAT 
ACGTATCAGC ATTTGCGGCT TATATTGAGT AAGTATGACG ACAACCAGAA GCTGGCTCAA 
CTGAGCGAGA ATGAGTACTT TAAAATCTAC ATCGACAGTC TCATGAAGAA GTCAAAGCGA 
GCGATCCGGC TCTTCAAAGA AGGCAAGGAG AGGATGTACG AGGAGCAGTC GCAGGACAGA 
CGGAATCTCA CAAAGCTGTC CCTTATCTTC AGTCACATGC TGGCAGAAAT CAAGGCGATC 
TTTCCCAATG GCCAGTTCCA GGGAGATAAC TTCCGGATCA CCAAAGCAGA TGCTGCCGAA 
TTCTGGAGGA AGTTTTTTGG AGACAAAACT ATCGTACCAT GGAAAGTCTT CAGACAGTGC 
CTGCATGAGG TCCATCAGAT CAGCTCTGGC CTGGAGGCCA TGGCTCTGAA GTCAACCATT 
GACTTAACTT GTAATGATTA CATCTCCGTG TTTGAATTTG ATATTTTTAC CAGGCTATTT 
CAGCCCTGGG GCTCTATTTT ACGGAATTGG AACTTCTTAG CTGTGACACA CCCGGGGTAC 
ATGGCATTTC TCACATATGA TGAAGTTAAA GCTCGACTAC AGAAATACAG CACCAAGCCT 
GGAAGCTACA TTTTCCGGTT AAGCTGCACT CGGCTGGGAC AATGGGCCAT TGGCTATGTG 
ACTGGGGACG GCAATATCCT ACAGACCATA CCTCATAATA AGCCCCTGTT CCAAGCCCTG 
ATTGATGGTA GCAGGGAAGG CTTTTACCTT TATCCAGATG GACGAAGCTA TAACCCTGAT 
TTAACCGGAT TATGTGAACC TACACCTCAT GATCATATAA AAGTTACACA GGAGCAATAT 
GAACTGTATT GTGAAATGGG CTCCACTTTT CAGCTGTGCA AGATCTGTGC AGAGAATGAC 
AAAGATGTCA AGATCGAGCC TTGCGGGCAT CTCATGTGCA CTTCGTGCCT TACCGCGTGG 
CAGGAGTCTG ATGGCCAAGG CTGCCCCTTC TGTCGCTGTG AGATAAAAGG AACCGAACCT 
ATCATCGTGG ATCCCTTTGA CCCCAGAGAC GAAGGCTCCA GGTGCTGCAG CATCATCGAC 
CCTTTCAGCA TCCCCATGCT CGACTTGGAT GATGACGATG ATCGAGAGGA GTCTCTGATG 
ATGAACCGGC TGGCGAGTGT TCGCAAGTGC ACAGACAGGC AGAACTCGCC AGTCACATCG 
CCAGGATCCT CACCCCTTGC CCAGAGAAGA AAGCCTCAGC CAGACCCTCT CCAGATCCCC 
CACCTCAGCC TGCCACCAGT GCCTCCCCGC CTGGACCTCA TTCAGAAAGG CATCGTGCGC 
TCTCCCTGTG GCAGCCCCAC GGGCTCCCCG AAGTCTTCTC CATGCATGGT TAGAAAACAA 
GACAAACCAC TCCCAGCACC CCCTCCTCCC TTGCGAGATC CTCCGCCTCC ACCAGAGCGG 
CCTCCGCCAA TCCCGCCTGA CAGTAGACTG AGCAGACACT TCCACCACGG AGAGGGTGTG 
CCTTCCAGGG ACCAGCCAAT GCCTCTTGAA GCCTGGTGCC CTCGGGATGC CTTCGGGACT 
AATCAGGTGA TGGGATGTCG CATCCTAGGG GATGGCTCTC CAAAGCCTGG CGTCACAGCA 
AACTCCAACT TAAATGGACG TCACAGTCGA ATGGGCTCTG ACCAGGTTCT TATGAGGAAA 
CACAGACGCC ACGATTTGCC TTCAGAAGGC GCCAAGGTCT TTTCCAATGG ACACCTTGCC 
CCTGAAGAAT ACGACGTTCC TCCTCGGCTT TCCCCTCCTC CTCCAGTCAC TGCCCTTCTC 
CCTAGCATAA AGTGTACTGG TCCAATAGCA AATTGTCTCT CCGAGAAAAC AAGAGACACA 
GTAGAAGAAG ATGATGATGA ATACAAGATT CCTTCATCCC ATCCTGTTTC CCTGAATTCA 
CAACCATCTC ATTGTCATAA TGTCAAACCT CCTGTTCGGT CTTGTGATAA TGGTCACTGT 
ATACTGAATG GAACTCATGG TACGCCTTCA GAGATGAAGA AATCAAACAT CCCAGATTTA 
GGCATCTATT TGAAGGGAGA TGTTTCTGAT TCAGCCTCTG ATCCAGTACC ATTACCACCT 
GCCAGGCCTC CACCCCGGGA CAACCTAAAG CATGGTTCTT CAGTCAACAG GACGCCCTCT 
GATTATGATC TTCTCATCCC TCCATTAGGT GAAGATGCTT TTGATGCCCT CCCCCCATCC 
CTTCCTCCTC CTCCACCTCC TGCAAGACAT AGTCTCATCG AGCATTCAAA ACCTCCAGGC 
TCCAGTAGCC GGCCTTCCTC AGGACAGGAC CTTTTCCTTC TTCCTTCAGA TCCCTTTTTT 
GACCCAGCAA GTGGCCAAGT TCCATTGCCT CCGGCCAGGA GAGCACCAGG AGATGGTGTC 
AAATCCAACA GAGCCTCCCA GGACTATGAC CAGCTCCCTT CATCTTCCGA TGGTTCGCAA 
GCACCAGCTA GACCCCCCAA ACCACGACCC CGAAGGACTG CACCAGAAAT TCATCACAGA 
AAGCCCCATG GGCCCGAGGC GGCACTGGAA AATGTGGATG CGAAAATTGC AAAACTCATG 
GGAGAGGGGT ATGCCTTTGA AGAGGTGAAG AGAGCCTTAG AGATCGCCCA GAATAACCTG 
GAAGTGGCCA GGAGCATACT TCGAGAATTC GCCTTCCCTC CTCCCGTCTC GCCACGTCTC 
AATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766848.1
CDS	265..3213
Translation	MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNSPPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDRRNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKPGSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPHDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCEIKGTEPIIVDPFDPRDEGSRCCSIIDPFSIPMLDLDDDDDREESLMMNRLASVRKCTDRQNSPVTSPGSSPLAQRRKPQPDPLQIPHLSLPPVPPRLDLIQKGIVRSPCGSPTGSPKSSPCMVRKQDKPLPAPPPPLRDPPPPPERPPPIPPDSRLSRHFHHGEGVPSRDQPMPLEAWCPRDAFGTNQVMGCRILGDGSPKPGVTANSNLNGRHSRMGSDQVLMRKHRRHDLPSEGAKVFSNGHLAPEEYDVPPRLSPPPPVTALLPSIKCTGPIANCLSEKTRDTVEEDDDEYKIPSSHPVSLNSQPSHCHNVKPPVRSCDNGHCILNGTHGTPSEMKKSNIPDLGIYLKGDVSDSASDPVPLPPARPPPRDNLKHGSSVNRTPSDYDLLIPPLGEDAFDALPPSLPPPPPPARHSLIEHSKPPGSSSRPSSGQDLFLLPSDPFFDPASGQVPLPPARRAPGDGVKSNRASQDYDQLPSSSDGSQAPARPPKPRPRRTAPEIHHRKPHGPEAALENVDAKIAKLMGEGYAFEEVKRALEIAQNNLEVARSILREFAFPPPVSPRLNL

Target ORF information:

RefSeq Version	XM_008768626.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Cbl proto-oncogene B (Cblb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768626.2

ORF Insert Sequence:

ATGGCAAATT CTATGAATGG CAGAAATCCT GGTGGTCGAG GAGGAAACCC CCGCAAAGGT 
CGAATTTTGG GGATTATTGA TGCCATTCAG GATGCAGTTG GACCCCCAAA GCAAGCTGCA 
GCTGACCGCA GGACAGTGGA GAAGACTTGG AAACTCATGG ACAAAGTGGT AAGACTGTGC 
CAAAATCCGA AACTTCAGTT GAAAAACAGC CCACCATATA TCCTCGACAT TTTACCTGAT 
ACGTATCAGC ATTTGCGGCT TATATTGAGT AAGTATGACG ACAACCAGAA GCTGGCTCAA 
CTGAGCGAGA ATGAGTACTT TAAAATCTAC ATCGACAGTC TCATGAAGAA GTCAAAGCGA 
GCGATCCGGC TCTTCAAAGA AGGCAAGGAG AGGATGTACG AGGAGCAGTC GCAGGACAGA 
CGGAATCTCA CAAAGCTGTC CCTTATCTTC AGTCACATGC TGGCAGAAAT CAAGGCGATC 
TTTCCCAATG GCCAGTTCCA GGGAGATAAC TTCCGGATCA CCAAAGCAGA TGCTGCCGAA 
TTCTGGAGGA AGTTTTTTGG AGACAAAACT ATCGTACCAT GGAAAGTCTT CAGACAGTGC 
CTGCATGAGG TCCATCAGAT CAGCTCTGGC CTGGAGGCCA TGGCTCTGAA GTCAACCATT 
GACTTAACTT GTAATGATTA CATCTCCGTG TTTGAATTTG ATATTTTTAC CAGGCTATTT 
CAGCCCTGGG GCTCTATTTT ACGGAATTGG AACTTCTTAG CTGTGACACA CCCGGGGTAC 
ATGGCATTTC TCACATATGA TGAAGTTAAA GCTCGACTAC AGAAATACAG CACCAAGCCT 
GGAAGCTACA TTTTCCGGTT AAGCTGCACT CGGCTGGGAC AATGGGCCAT TGGCTATGTG 
ACTGGGGACG GCAATATCCT ACAGACCATA CCTCATAATA AGCCCCTGTT CCAAGCCCTG 
ATTGATGGTA GCAGGGAAGG CTTTTACCTT TATCCAGATG GACGAAGCTA TAACCCTGAT 
TTAACCGGAT TATGTGAACC TACACCTCAT GATCATATAA AAGTTACACA GGAGCAATAT 
GAACTGTATT GTGAAATGGG CTCCACTTTT CAGCTGTGCA AGATCTGTGC AGAGAATGAC 
AAAGATGTCA AGATCGAGCC TTGCGGGCAT CTCATGTGCA CTTCGTGCCT TACCGCGTGG 
CAGGAGTCTG ATGGCCAAGG CTGCCCCTTC TGTCGCTGTG AGATAAAAGG AACCGAACCT 
ATCATCGTGG ATCCCTTTGA CCCCAGAGAC GAAGGCTCCA GGTGCTGCAG CATCATCGAC 
CCTTTCAGCA TCCCCATGCT CGACTTGGAT GATGACGATG ATCGAGAGGA GTCTCTGATG 
ATGAACCGGC TGGCGAGTGT TCGCAAGTGC ACAGACAGGC AGAACTCGCC AGTCACATCG 
CCAGGATCCT CACCCCTTGC CCAGAGAAGA AAGCCTCAGC CAGACCCTCT CCAGATCCCC 
CACCTCAGCC TGCCACCAGT GCCTCCCCGC CTGGACCTCA TTCAGAAAGG CATCGTGCGC 
TCTCCCTGTG GCAGCCCCAC GGGCTCCCCG AAGTCTTCTC CATGCATGGT TAGAAAACAA 
GACAAACCAC TCCCAGCACC CCCTCCTCCC TTGCGAGATC CTCCGCCTCC ACCAGAGCGG 
CCTCCGCCAA TCCCGCCTGA CAGTAGACTG AGCAGACACT TCCACCACGG AGAGGGTGTG 
CCTTCCAGGG ACCAGCCAAT GCCTCTTGAA GCCTGGTGCC CTCGGGATGC CTTCGGGACT 
AATCAGGTGA TGGGATGTCG CATCCTAGGG GATGGCTCTC CAAAGCCTGG CGTCACAGCA 
AACTCCAACT TAAATGGACG TCACAGTCGA ATGGGCTCTG ACCAGGTTCT TATGAGGAAA 
CACAGACGCC ACGATTTGCC TTCAGAAGGC GCCAAGGTCT TTTCCAATGG ACACCTTGCC 
CCTGAAGAAT ACGACGTTCC TCCTCGGCTT TCCCCTCCTC CTCCAGTCAC TGCCCTTCTC 
CCTAGCATAA AGTGTACTGG TCCAATAGCA AATTGTCTCT CCGAGAAAAC AAGAGACACA 
GTAGAAGAAG ATGATGATGA ATACAAGATT CCTTCATCCC ATCCTGTTTC CCTGAATTCA 
CAACCATCTC ATTGTCATAA TGTCAAACCT CCTGTTCGGT CTTGTGATAA TGGTCACTGT 
ATACTGAATG GAACTCATGG TACGCCTTCA GAGATGAAGA AATCAAACAT CCCAGATTTA 
GGCATCTATT TGAAGGGAGA TGTTTCTGAT TCAGCCTCTG ATCCAGTACC ATTACCACCT 
GCCAGGCCTC CACCCCGGGA CAACCTAAAG CATGGTTCTT CAGTCAACAG GACGCCCTCT 
GATTATGATC TTCTCATCCC TCCATTAGGT GAAGATGCTT TTGATGCCCT CCCCCCATCC 
CTTCCTCCTC CTCCACCTCC TGCAAGACAT AGTCTCATCG AGCATTCAAA ACCTCCAGGC 
TCCAGTAGCC GGCCTTCCTC AGGACAGGAC CTTTTCCTTC TTCCTTCAGA TCCCTTTTTT 
GACCCAGCAA GTGGCCAAGT TCCATTGCCT CCGGCCAGGA GAGCACCAGG AGATGGTGTC 
AAATCCAACA GAGCCTCCCA GGACTATGAC CAGCTCCCTT CATCTTCCGA TGGTTCGCAA 
GCACCAGCTA GACCCCCCAA ACCACGACCC CGAAGGACTG CACCAGAAAT TCATCACAGA 
AAGCCCCATG GGCCCGAGGC GGCACTGGAA AATGTGGATG CGAAAATTGC AAAACTCATG 
GGAGAGGGGT ATGCCTTTGA AGAGGTGAAG AGAGCCTTAG AGATCGCCCA GAATAACCTG 
GAAGTGGCCA GGAGCATACT TCGAGAATTC GCCTTCCCTC CTCCCGTCTC GCCACGTCTC 
AATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cblb ( XM_006248259.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248321.1 Rattus norvegicus Cbl proto-oncogene B (Cblb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa34235
Clone ID Related Accession (Same CDS sequence)	XM_006248258.3 , XM_008768626.2 , XM_006248259.3
Accession Version	XM_006248259.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase CBL-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248259.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATT CTATGAATGG CAGAAATCCT GGTGGTCGAG GAGGAAACCC CCGCAAAGGT 
CGAATTTTGG GGATTATTGA TGCCATTCAG GATGCAGTTG GACCCCCAAA GCAAGCTGCA 
GCTGACCGCA GGACAGTGGA GAAGACTTGG AAACTCATGG ACAAAGTGGT AAGACTGTGC 
CAAAATCCGA AACTTCAGTT GAAAAACAGC CCACCATATA TCCTCGACAT TTTACCTGAT 
ACGTATCAGC ATTTGCGGCT TATATTGAGT AAGTATGACG ACAACCAGAA GCTGGCTCAA 
CTGAGCGAGA ATGAGTACTT TAAAATCTAC ATCGACAGTC TCATGAAGAA GTCAAAGCGA 
GCGATCCGGC TCTTCAAAGA AGGCAAGGAG AGGATGTACG AGGAGCAGTC GCAGGACAGA 
CGGAATCTCA CAAAGCTGTC CCTTATCTTC AGTCACATGC TGGCAGAAAT CAAGGCGATC 
TTTCCCAATG GCCAGTTCCA GGGAGATAAC TTCCGGATCA CCAAAGCAGA TGCTGCCGAA 
TTCTGGAGGA AGTTTTTTGG AGACAAAACT ATCGTACCAT GGAAAGTCTT CAGACAGTGC 
CTGCATGAGG TCCATCAGAT CAGCTCTGGC CTGGAGGCCA TGGCTCTGAA GTCAACCATT 
GACTTAACTT GTAATGATTA CATCTCCGTG TTTGAATTTG ATATTTTTAC CAGGCTATTT 
CAGCCCTGGG GCTCTATTTT ACGGAATTGG AACTTCTTAG CTGTGACACA CCCGGGGTAC 
ATGGCATTTC TCACATATGA TGAAGTTAAA GCTCGACTAC AGAAATACAG CACCAAGCCT 
GGAAGCTACA TTTTCCGGTT AAGCTGCACT CGGCTGGGAC AATGGGCCAT TGGCTATGTG 
ACTGGGGACG GCAATATCCT ACAGACCATA CCTCATAATA AGCCCCTGTT CCAAGCCCTG 
ATTGATGGTA GCAGGGAAGG CTTTTACCTT TATCCAGATG GACGAAGCTA TAACCCTGAT 
TTAACCGGAT TATGTGAACC TACACCTCAT GATCATATAA AAGTTACACA GGAGCAATAT 
GAACTGTATT GTGAAATGGG CTCCACTTTT CAGCTGTGCA AGATCTGTGC AGAGAATGAC 
AAAGATGTCA AGATCGAGCC TTGCGGGCAT CTCATGTGCA CTTCGTGCCT TACCGCGTGG 
CAGGAGTCTG ATGGCCAAGG CTGCCCCTTC TGTCGCTGTG AGATAAAAGG AACCGAACCT 
ATCATCGTGG ATCCCTTTGA CCCCAGAGAC GAAGGCTCCA GGTGCTGCAG CATCATCGAC 
CCTTTCAGCA TCCCCATGCT CGACTTGGAT GATGACGATG ATCGAGAGGA GTCTCTGATG 
ATGAACCGGC TGGCGAGTGT TCGCAAGTGC ACAGACAGGC AGAACTCGCC AGTCACATCG 
CCAGGATCCT CACCCCTTGC CCAGAGAAGA AAGCCTCAGC CAGACCCTCT CCAGATCCCC 
CACCTCAGCC TGCCACCAGT GCCTCCCCGC CTGGACCTCA TTCAGAAAGG CATCGTGCGC 
TCTCCCTGTG GCAGCCCCAC GGGCTCCCCG AAGTCTTCTC CATGCATGGT TAGAAAACAA 
GACAAACCAC TCCCAGCACC CCCTCCTCCC TTGCGAGATC CTCCGCCTCC ACCAGAGCGG 
CCTCCGCCAA TCCCGCCTGA CAGTAGACTG AGCAGACACT TCCACCACGG AGAGGGTGTG 
CCTTCCAGGG ACCAGCCAAT GCCTCTTGAA GCCTGGTGCC CTCGGGATGC CTTCGGGACT 
AATCAGGTGA TGGGATGTCG CATCCTAGGG GATGGCTCTC CAAAGCCTGG CGTCACAGCA 
AACTCCAACT TAAATGGACG TCACAGTCGA ATGGGCTCTG ACCAGGTTCT TATGAGGAAA 
CACAGACGCC ACGATTTGCC TTCAGAAGGC GCCAAGGTCT TTTCCAATGG ACACCTTGCC 
CCTGAAGAAT ACGACGTTCC TCCTCGGCTT TCCCCTCCTC CTCCAGTCAC TGCCCTTCTC 
CCTAGCATAA AGTGTACTGG TCCAATAGCA AATTGTCTCT CCGAGAAAAC AAGAGACACA 
GTAGAAGAAG ATGATGATGA ATACAAGATT CCTTCATCCC ATCCTGTTTC CCTGAATTCA 
CAACCATCTC ATTGTCATAA TGTCAAACCT CCTGTTCGGT CTTGTGATAA TGGTCACTGT 
ATACTGAATG GAACTCATGG TACGCCTTCA GAGATGAAGA AATCAAACAT CCCAGATTTA 
GGCATCTATT TGAAGGGAGA TGTTTCTGAT TCAGCCTCTG ATCCAGTACC ATTACCACCT 
GCCAGGCCTC CACCCCGGGA CAACCTAAAG CATGGTTCTT CAGTCAACAG GACGCCCTCT 
GATTATGATC TTCTCATCCC TCCATTAGGT GAAGATGCTT TTGATGCCCT CCCCCCATCC 
CTTCCTCCTC CTCCACCTCC TGCAAGACAT AGTCTCATCG AGCATTCAAA ACCTCCAGGC 
TCCAGTAGCC GGCCTTCCTC AGGACAGGAC CTTTTCCTTC TTCCTTCAGA TCCCTTTTTT 
GACCCAGCAA GTGGCCAAGT TCCATTGCCT CCGGCCAGGA GAGCACCAGG AGATGGTGTC 
AAATCCAACA GAGCCTCCCA GGACTATGAC CAGCTCCCTT CATCTTCCGA TGGTTCGCAA 
GCACCAGCTA GACCCCCCAA ACCACGACCC CGAAGGACTG CACCAGAAAT TCATCACAGA 
AAGCCCCATG GGCCCGAGGC GGCACTGGAA AATGTGGATG CGAAAATTGC AAAACTCATG 
GGAGAGGGGT ATGCCTTTGA AGAGGTGAAG AGAGCCTTAG AGATCGCCCA GAATAACCTG 
GAAGTGGCCA GGAGCATACT TCGAGAATTC GCCTTCCCTC CTCCCGTCTC GCCACGTCTC 
AATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248321.1
CDS	178..3126
Translation	MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNSPPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDRRNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKPGSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPHDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCEIKGTEPIIVDPFDPRDEGSRCCSIIDPFSIPMLDLDDDDDREESLMMNRLASVRKCTDRQNSPVTSPGSSPLAQRRKPQPDPLQIPHLSLPPVPPRLDLIQKGIVRSPCGSPTGSPKSSPCMVRKQDKPLPAPPPPLRDPPPPPERPPPIPPDSRLSRHFHHGEGVPSRDQPMPLEAWCPRDAFGTNQVMGCRILGDGSPKPGVTANSNLNGRHSRMGSDQVLMRKHRRHDLPSEGAKVFSNGHLAPEEYDVPPRLSPPPPVTALLPSIKCTGPIANCLSEKTRDTVEEDDDEYKIPSSHPVSLNSQPSHCHNVKPPVRSCDNGHCILNGTHGTPSEMKKSNIPDLGIYLKGDVSDSASDPVPLPPARPPPRDNLKHGSSVNRTPSDYDLLIPPLGEDAFDALPPSLPPPPPPARHSLIEHSKPPGSSSRPSSGQDLFLLPSDPFFDPASGQVPLPPARRAPGDGVKSNRASQDYDQLPSSSDGSQAPARPPKPRPRRTAPEIHHRKPHGPEAALENVDAKIAKLMGEGYAFEEVKRALEIAQNNLEVARSILREFAFPPPVSPRLNL

Target ORF information:

RefSeq Version	XM_006248259.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Cbl proto-oncogene B (Cblb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248259.3

ORF Insert Sequence:

ATGGCAAATT CTATGAATGG CAGAAATCCT GGTGGTCGAG GAGGAAACCC CCGCAAAGGT 
CGAATTTTGG GGATTATTGA TGCCATTCAG GATGCAGTTG GACCCCCAAA GCAAGCTGCA 
GCTGACCGCA GGACAGTGGA GAAGACTTGG AAACTCATGG ACAAAGTGGT AAGACTGTGC 
CAAAATCCGA AACTTCAGTT GAAAAACAGC CCACCATATA TCCTCGACAT TTTACCTGAT 
ACGTATCAGC ATTTGCGGCT TATATTGAGT AAGTATGACG ACAACCAGAA GCTGGCTCAA 
CTGAGCGAGA ATGAGTACTT TAAAATCTAC ATCGACAGTC TCATGAAGAA GTCAAAGCGA 
GCGATCCGGC TCTTCAAAGA AGGCAAGGAG AGGATGTACG AGGAGCAGTC GCAGGACAGA 
CGGAATCTCA CAAAGCTGTC CCTTATCTTC AGTCACATGC TGGCAGAAAT CAAGGCGATC 
TTTCCCAATG GCCAGTTCCA GGGAGATAAC TTCCGGATCA CCAAAGCAGA TGCTGCCGAA 
TTCTGGAGGA AGTTTTTTGG AGACAAAACT ATCGTACCAT GGAAAGTCTT CAGACAGTGC 
CTGCATGAGG TCCATCAGAT CAGCTCTGGC CTGGAGGCCA TGGCTCTGAA GTCAACCATT 
GACTTAACTT GTAATGATTA CATCTCCGTG TTTGAATTTG ATATTTTTAC CAGGCTATTT 
CAGCCCTGGG GCTCTATTTT ACGGAATTGG AACTTCTTAG CTGTGACACA CCCGGGGTAC 
ATGGCATTTC TCACATATGA TGAAGTTAAA GCTCGACTAC AGAAATACAG CACCAAGCCT 
GGAAGCTACA TTTTCCGGTT AAGCTGCACT CGGCTGGGAC AATGGGCCAT TGGCTATGTG 
ACTGGGGACG GCAATATCCT ACAGACCATA CCTCATAATA AGCCCCTGTT CCAAGCCCTG 
ATTGATGGTA GCAGGGAAGG CTTTTACCTT TATCCAGATG GACGAAGCTA TAACCCTGAT 
TTAACCGGAT TATGTGAACC TACACCTCAT GATCATATAA AAGTTACACA GGAGCAATAT 
GAACTGTATT GTGAAATGGG CTCCACTTTT CAGCTGTGCA AGATCTGTGC AGAGAATGAC 
AAAGATGTCA AGATCGAGCC TTGCGGGCAT CTCATGTGCA CTTCGTGCCT TACCGCGTGG 
CAGGAGTCTG ATGGCCAAGG CTGCCCCTTC TGTCGCTGTG AGATAAAAGG AACCGAACCT 
ATCATCGTGG ATCCCTTTGA CCCCAGAGAC GAAGGCTCCA GGTGCTGCAG CATCATCGAC 
CCTTTCAGCA TCCCCATGCT CGACTTGGAT GATGACGATG ATCGAGAGGA GTCTCTGATG 
ATGAACCGGC TGGCGAGTGT TCGCAAGTGC ACAGACAGGC AGAACTCGCC AGTCACATCG 
CCAGGATCCT CACCCCTTGC CCAGAGAAGA AAGCCTCAGC CAGACCCTCT CCAGATCCCC 
CACCTCAGCC TGCCACCAGT GCCTCCCCGC CTGGACCTCA TTCAGAAAGG CATCGTGCGC 
TCTCCCTGTG GCAGCCCCAC GGGCTCCCCG AAGTCTTCTC CATGCATGGT TAGAAAACAA 
GACAAACCAC TCCCAGCACC CCCTCCTCCC TTGCGAGATC CTCCGCCTCC ACCAGAGCGG 
CCTCCGCCAA TCCCGCCTGA CAGTAGACTG AGCAGACACT TCCACCACGG AGAGGGTGTG 
CCTTCCAGGG ACCAGCCAAT GCCTCTTGAA GCCTGGTGCC CTCGGGATGC CTTCGGGACT 
AATCAGGTGA TGGGATGTCG CATCCTAGGG GATGGCTCTC CAAAGCCTGG CGTCACAGCA 
AACTCCAACT TAAATGGACG TCACAGTCGA ATGGGCTCTG ACCAGGTTCT TATGAGGAAA 
CACAGACGCC ACGATTTGCC TTCAGAAGGC GCCAAGGTCT TTTCCAATGG ACACCTTGCC 
CCTGAAGAAT ACGACGTTCC TCCTCGGCTT TCCCCTCCTC CTCCAGTCAC TGCCCTTCTC 
CCTAGCATAA AGTGTACTGG TCCAATAGCA AATTGTCTCT CCGAGAAAAC AAGAGACACA 
GTAGAAGAAG ATGATGATGA ATACAAGATT CCTTCATCCC ATCCTGTTTC CCTGAATTCA 
CAACCATCTC ATTGTCATAA TGTCAAACCT CCTGTTCGGT CTTGTGATAA TGGTCACTGT 
ATACTGAATG GAACTCATGG TACGCCTTCA GAGATGAAGA AATCAAACAT CCCAGATTTA 
GGCATCTATT TGAAGGGAGA TGTTTCTGAT TCAGCCTCTG ATCCAGTACC ATTACCACCT 
GCCAGGCCTC CACCCCGGGA CAACCTAAAG CATGGTTCTT CAGTCAACAG GACGCCCTCT 
GATTATGATC TTCTCATCCC TCCATTAGGT GAAGATGCTT TTGATGCCCT CCCCCCATCC 
CTTCCTCCTC CTCCACCTCC TGCAAGACAT AGTCTCATCG AGCATTCAAA ACCTCCAGGC 
TCCAGTAGCC GGCCTTCCTC AGGACAGGAC CTTTTCCTTC TTCCTTCAGA TCCCTTTTTT 
GACCCAGCAA GTGGCCAAGT TCCATTGCCT CCGGCCAGGA GAGCACCAGG AGATGGTGTC 
AAATCCAACA GAGCCTCCCA GGACTATGAC CAGCTCCCTT CATCTTCCGA TGGTTCGCAA 
GCACCAGCTA GACCCCCCAA ACCACGACCC CGAAGGACTG CACCAGAAAT TCATCACAGA 
AAGCCCCATG GGCCCGAGGC GGCACTGGAA AATGTGGATG CGAAAATTGC AAAACTCATG 
GGAGAGGGGT ATGCCTTTGA AGAGGTGAAG AGAGCCTTAG AGATCGCCCA GAATAACCTG 
GAAGTGGCCA GGAGCATACT TCGAGAATTC GCCTTCCCTC CTCCCGTCTC GCCACGTCTC 
AATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cblb ( XM_006248260.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248322.1 Rattus norvegicus Cbl proto-oncogene B (Cblb), transcript variant X4, mRNA.

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CloneID	ORa34236
Clone ID Related Accession (Same CDS sequence)	XM_006248260.3
Accession Version	XM_006248260.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase CBL-B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248260.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATT CTATGAATGG CAGAAATCCT GGTGGTCGAG GAGGAAACCC CCGCAAAGGT 
CGAATTTTGG GGATTATTGA TGCCATTCAG GATGCAGTTG GACCCCCAAA GCAAGCTGCA 
GCTGACCGCA GGACAGTGGA GAAGACTTGG AAACTCATGG ACAAAGTGGT AAGACTGTGC 
CAAAATCCGA AACTTCAGTT GAAAAACAGC CCACCATATA TCCTCGACAT TTTACCTGAT 
ACGTATCAGC ATTTGCGGCT TATATTGAGT AAGTATGACG ACAACCAGAA GCTGGCTCAA 
CTGAGCGAGA ATGAGTACTT TAAAATCTAC ATCGACAGTC TCATGAAGAA GTCAAAGCGA 
GCGATCCGGC TCTTCAAAGA AGGCAAGGAG AGGATGTACG AGGAGCAGTC GCAGGACAGA 
CGGAATCTCA CAAAGCTGTC CCTTATCTTC AGTCACATGC TGGCAGAAAT CAAGGCGATC 
TTTCCCAATG GCCAGTTCCA GGGAGATAAC TTCCGGATCA CCAAAGCAGA TGCTGCCGAA 
TTCTGGAGGA AGTTTTTTGG AGACAAAACT ATCGTACCAT GGAAAGTCTT CAGACAGTGC 
CTGCATGAGG TCCATCAGAT CAGCTCTGGC CTGGAGGCCA TGGCTCTGAA GTCAACCATT 
GACTTAACTT GTAATGATTA CATCTCCGTG TTTGAATTTG ATATTTTTAC CAGGCTATTT 
CAGCCCTGGG GCTCTATTTT ACGGAATTGG AACTTCTTAG CTGTGACACA CCCGGGGTAC 
ATGGCATTTC TCACATATGA TGAAGTTAAA GCTCGACTAC AGAAATACAG CACCAAGCCT 
GGAAGCTACA TTTTCCGGTT AAGCTGCACT CGGCTGGGAC AATGGGCCAT TGGCTATGTG 
ACTGGGGACG GCAATATCCT ACAGACCATA CCTCATAATA AGCCCCTGTT CCAAGCCCTG 
ATTGATGGTA GCAGGGAAGG CTTTTACCTT TATCCAGATG GACGAAGCTA TAACCCTGAT 
TTAACCGGAT TATGTGAACC TACACCTCAT GATCATATAA AAGTTACACA GGAGCAATAT 
GAACTGTATT GTGAAATGGG CTCCACTTTT CAGCTGTGCA AGATCTGTGC AGAGAATGAC 
AAAGATGTCA AGATCGAGCC TTGCGGGCAT CTCATGTGCA CTTCGTGCCT TACCGCGTGG 
CAGGAGTCTG ATGGCCAAGG CTGCCCCTTC TGTCGCTGTG AGATAAAAGG AACCGAACCT 
ATCATCGTGG ATCCCTTTGA CCCCAGAGAC GAAGGCTCCA GGTGCTGCAG CATCATCGAC 
CCTTTCAGCA TCCCCATGCT CGACTTGGAT GATGACGATG ATCGAGAGGA GTCTCTGATG 
ATGAACCGGC TGGCGAGTGT TCGCAAGTGC ACAGACAGGC AGAACTCGCC AGTCACATCG 
CCAGGATCCT CACCCCTTGC CCAGAGAAGA AAGCCTCAGC CAGACCCTCT CCAGATCCCC 
CACCTCAGCC TGCCACCAGT GCCTCCCCGC CTGGACCTCA TTCAGAAAGG CATCGTGCGC 
TCTCCCTGTG GCAGCCCCAC GGGCTCCCCG AAGGTCTTTT CCAATGGACA CCTTGCCCCT 
GAAGAATACG ACGTTCCTCC TCGGCTTTCC CCTCCTCCTC CAGTCACTGC CCTTCTCCCT 
AGCATAAAGT GTACTGGTCC AATAGCAAAT TGTCTCTCCG AGAAAACAAG AGACACAGTA 
GAAGAAGATG ATGATGAATA CAAGATTCCT TCATCCCATC CTGTTTCCCT GAATTCACAA 
CCATCTCATT GTCATAATGT CAAACCTCCT GTTCGGTCTT GTGATAATGG TCACTGTATA 
CTGAATGGAA CTCATGGTAC GCCTTCAGAG ATGAAGAAAT CAAACATCCC AGATTTAGGC 
ATCTATTTGA AGGGAGATGT TTCTGATTCA GCCTCTGATC CAGTACCATT ACCACCTGCC 
AGGCCTCCAC CCCGGGACAA CCTAAAGCAT GGTTCTTCAG TCAACAGGAC GCCCTCTGAT 
TATGATCTTC TCATCCCTCC ATTAGGTGAA GATGCTTTTG ATGCCCTCCC CCCATCCCTT 
CCTCCTCCTC CACCTCCTGC AAGACATAGT CTCATCGAGC ATTCAAAACC TCCAGGCTCC 
AGTAGCCGGC CTTCCTCAGG ACAGGACCTT TTCCTTCTTC CTTCAGATCC CTTTTTTGAC 
CCAGCAAGTG GCCAAGTTCC ATTGCCTCCG GCCAGGAGAG CACCAGGAGA TGGTGTCAAA 
TCCAACAGAG CCTCCCAGGA CTATGACCAG CTCCCTTCAT CTTCCGATGG TTCGCAAGCA 
CCAGCTAGAC CCCCCAAACC ACGACCCCGA AGGACTGCAC CAGAAATTCA TCACAGAAAG 
CCCCATGGGC CCGAGGCGGC ACTGGAAAAT GTGGATGCGA AAATTGCAAA ACTCATGGGA 
GAGGGGTATG CCTTTGAAGA GGTGAAGAGA GCCTTAGAGA TCGCCCAGAA TAACCTGGAA 
GTGGCCAGGA GCATACTTCG AGAATTCGCC TTCCCTCCTC CCGTCTCGCC ACGTCTCAAT 
CTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248322.1
CDS	103..2688
Translation	MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNSPPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDRRNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKPGSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPHDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCEIKGTEPIIVDPFDPRDEGSRCCSIIDPFSIPMLDLDDDDDREESLMMNRLASVRKCTDRQNSPVTSPGSSPLAQRRKPQPDPLQIPHLSLPPVPPRLDLIQKGIVRSPCGSPTGSPKVFSNGHLAPEEYDVPPRLSPPPPVTALLPSIKCTGPIANCLSEKTRDTVEEDDDEYKIPSSHPVSLNSQPSHCHNVKPPVRSCDNGHCILNGTHGTPSEMKKSNIPDLGIYLKGDVSDSASDPVPLPPARPPPRDNLKHGSSVNRTPSDYDLLIPPLGEDAFDALPPSLPPPPPPARHSLIEHSKPPGSSSRPSSGQDLFLLPSDPFFDPASGQVPLPPARRAPGDGVKSNRASQDYDQLPSSSDGSQAPARPPKPRPRRTAPEIHHRKPHGPEAALENVDAKIAKLMGEGYAFEEVKRALEIAQNNLEVARSILREFAFPPPVSPRLNL

Target ORF information:

RefSeq Version	XM_006248260.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Cbl proto-oncogene B (Cblb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248260.3

ORF Insert Sequence:

ATGGCAAATT CTATGAATGG CAGAAATCCT GGTGGTCGAG GAGGAAACCC CCGCAAAGGT 
CGAATTTTGG GGATTATTGA TGCCATTCAG GATGCAGTTG GACCCCCAAA GCAAGCTGCA 
GCTGACCGCA GGACAGTGGA GAAGACTTGG AAACTCATGG ACAAAGTGGT AAGACTGTGC 
CAAAATCCGA AACTTCAGTT GAAAAACAGC CCACCATATA TCCTCGACAT TTTACCTGAT 
ACGTATCAGC ATTTGCGGCT TATATTGAGT AAGTATGACG ACAACCAGAA GCTGGCTCAA 
CTGAGCGAGA ATGAGTACTT TAAAATCTAC ATCGACAGTC TCATGAAGAA GTCAAAGCGA 
GCGATCCGGC TCTTCAAAGA AGGCAAGGAG AGGATGTACG AGGAGCAGTC GCAGGACAGA 
CGGAATCTCA CAAAGCTGTC CCTTATCTTC AGTCACATGC TGGCAGAAAT CAAGGCGATC 
TTTCCCAATG GCCAGTTCCA GGGAGATAAC TTCCGGATCA CCAAAGCAGA TGCTGCCGAA 
TTCTGGAGGA AGTTTTTTGG AGACAAAACT ATCGTACCAT GGAAAGTCTT CAGACAGTGC 
CTGCATGAGG TCCATCAGAT CAGCTCTGGC CTGGAGGCCA TGGCTCTGAA GTCAACCATT 
GACTTAACTT GTAATGATTA CATCTCCGTG TTTGAATTTG ATATTTTTAC CAGGCTATTT 
CAGCCCTGGG GCTCTATTTT ACGGAATTGG AACTTCTTAG CTGTGACACA CCCGGGGTAC 
ATGGCATTTC TCACATATGA TGAAGTTAAA GCTCGACTAC AGAAATACAG CACCAAGCCT 
GGAAGCTACA TTTTCCGGTT AAGCTGCACT CGGCTGGGAC AATGGGCCAT TGGCTATGTG 
ACTGGGGACG GCAATATCCT ACAGACCATA CCTCATAATA AGCCCCTGTT CCAAGCCCTG 
ATTGATGGTA GCAGGGAAGG CTTTTACCTT TATCCAGATG GACGAAGCTA TAACCCTGAT 
TTAACCGGAT TATGTGAACC TACACCTCAT GATCATATAA AAGTTACACA GGAGCAATAT 
GAACTGTATT GTGAAATGGG CTCCACTTTT CAGCTGTGCA AGATCTGTGC AGAGAATGAC 
AAAGATGTCA AGATCGAGCC TTGCGGGCAT CTCATGTGCA CTTCGTGCCT TACCGCGTGG 
CAGGAGTCTG ATGGCCAAGG CTGCCCCTTC TGTCGCTGTG AGATAAAAGG AACCGAACCT 
ATCATCGTGG ATCCCTTTGA CCCCAGAGAC GAAGGCTCCA GGTGCTGCAG CATCATCGAC 
CCTTTCAGCA TCCCCATGCT CGACTTGGAT GATGACGATG ATCGAGAGGA GTCTCTGATG 
ATGAACCGGC TGGCGAGTGT TCGCAAGTGC ACAGACAGGC AGAACTCGCC AGTCACATCG 
CCAGGATCCT CACCCCTTGC CCAGAGAAGA AAGCCTCAGC CAGACCCTCT CCAGATCCCC 
CACCTCAGCC TGCCACCAGT GCCTCCCCGC CTGGACCTCA TTCAGAAAGG CATCGTGCGC 
TCTCCCTGTG GCAGCCCCAC GGGCTCCCCG AAGGTCTTTT CCAATGGACA CCTTGCCCCT 
GAAGAATACG ACGTTCCTCC TCGGCTTTCC CCTCCTCCTC CAGTCACTGC CCTTCTCCCT 
AGCATAAAGT GTACTGGTCC AATAGCAAAT TGTCTCTCCG AGAAAACAAG AGACACAGTA 
GAAGAAGATG ATGATGAATA CAAGATTCCT TCATCCCATC CTGTTTCCCT GAATTCACAA 
CCATCTCATT GTCATAATGT CAAACCTCCT GTTCGGTCTT GTGATAATGG TCACTGTATA 
CTGAATGGAA CTCATGGTAC GCCTTCAGAG ATGAAGAAAT CAAACATCCC AGATTTAGGC 
ATCTATTTGA AGGGAGATGT TTCTGATTCA GCCTCTGATC CAGTACCATT ACCACCTGCC 
AGGCCTCCAC CCCGGGACAA CCTAAAGCAT GGTTCTTCAG TCAACAGGAC GCCCTCTGAT 
TATGATCTTC TCATCCCTCC ATTAGGTGAA GATGCTTTTG ATGCCCTCCC CCCATCCCTT 
CCTCCTCCTC CACCTCCTGC AAGACATAGT CTCATCGAGC ATTCAAAACC TCCAGGCTCC 
AGTAGCCGGC CTTCCTCAGG ACAGGACCTT TTCCTTCTTC CTTCAGATCC CTTTTTTGAC 
CCAGCAAGTG GCCAAGTTCC ATTGCCTCCG GCCAGGAGAG CACCAGGAGA TGGTGTCAAA 
TCCAACAGAG CCTCCCAGGA CTATGACCAG CTCCCTTCAT CTTCCGATGG TTCGCAAGCA 
CCAGCTAGAC CCCCCAAACC ACGACCCCGA AGGACTGCAC CAGAAATTCA TCACAGAAAG 
CCCCATGGGC CCGAGGCGGC ACTGGAAAAT GTGGATGCGA AAATTGCAAA ACTCATGGGA 
GAGGGGTATG CCTTTGAAGA GGTGAAGAGA GCCTTAGAGA TCGCCCAGAA TAACCTGGAA 
GTGGCCAGGA GCATACTTCG AGAATTCGCC TTCCCTCCTC CCGTCTCGCC ACGTCTCAAT 
CTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increased expression of the Cbl family of E3 ubiquitin ligases decreases Interleukin-2 production in a rat model of peripheral neuropathy.
BMC anesthesiology18(1)87(2018 07)
Jeong JS,Kim HY,Shin BS,Lee AR,Yoon JH,Hahm TS,Lee JE

Reactive oxygen species upregulate expression of muscle atrophy-associated ubiquitin ligase Cbl-b in rat L6 skeletal muscle cells.
American journal of physiology. Cell physiology314(6)C721-C731(2018 06)
Uchida T,Sakashita Y,Kitahata K,Yamashita Y,Tomida C,Kimori Y,Komatsu A,Hirasaka K,Ohno A,Nakao R,Higashitani A,Higashibata A,Ishioka N,Shimazu T,Kobayashi T,Okumura Y,Choi I,Oarada M,Mills EM,Teshima-Kondo S,Takeda S,Tanaka E,Tanaka K,Sokabe M,Nikawa T

IFT20 modulates ciliary PDGFRα signaling by regulating the stability of Cbl E3 ubiquitin ligases.
The Journal of cell biology217(1)151-161(2018 01)
Schmid FM,Schou KB,Vilhelm MJ,Holm MS,Breslin L,Farinelli P,Larsen LA,Andersen JS,Pedersen LB,Christensen ST

Cbl-b promotes chemotherapy-induced apoptosis in rat basophilic leukemia cells by suppressing PI3K/Akt activation and enhancing MEK/ERK activation.
Molecular and cellular biochemistry340(1-2)107-14(2010 Jul)
Qu X,Li Y,Liu J,Xu L,Zhang Y,Hu X,Hou K,Liu Y

Ubiquitin ligase Cbl-b is a negative regulator for insulin-like growth factor 1 signaling during muscle atrophy caused by unloading.
Molecular and cellular biology29(17)4798-811(2009 Sep)
Nakao R,Hirasaka K,Goto J,Ishidoh K,Yamada C,Ohno A,Okumura Y,Nonaka I,Yasutomo K,Baldwin KM,Kominami E,Higashibata A,Nagano K,Tanaka K,Yasui N,Mills EM,Takeda S,Nikawa T

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Cblb cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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