Atp5me cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp5me
Entrez Gene ID	140608
Full Name	ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E
Synonyms	Atp5k
General protein information	Preferred Names ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E Names ATP synthase subunit e, mitochondrial ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e ATPase subunit e
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22
Summary	subunit e of mitochondrial H(+)-ATP synthase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_080481.1	NP_536729.1	ATP synthase subunit e, mitochondrial

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Atp5k	NP_031533.2
Rattus norvegicus (Norway rat)	Atp5i	NP_536729.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of two proteins associated with mammalian ATP synthase.
Meyer B, Wittig I, Trifilieff E, Karas M, Schägger H
Molecular & cellular proteomics : MCP6(10)1690-9(2007 Oct)

A functionally active human F1F0 ATPase can be purified by immunocapture from heart tissue and fibroblast cell lines. Subunit structure and activity studies.
Aggeler R, Coons J, Taylor SW, Ghosh SS, Garcia JJ, Capaldi RA, Marusich MF
The Journal of biological chemistry277(37)33906-12(2002 Sep)

Mitochondrial F(0)F(1) ATP synthase. Subunit regions on the F1 motor shielded by F(0), Functional significance, and evidence for an involvement of the unique F(0) subunit F(6).
Ko YH, Hullihen J, Hong S, Pedersen PL
The Journal of biological chemistry275(42)32931-9(2000 Oct)

Complete amino acid sequence of subunit e of rat liver mitochondrial H(+)-ATP synthase.
Higuti T, Kuroiwa K, Kawamura Y, Yoshihara Y
Biochemistry31(49)12451-4(1992 Dec)

A simple, rapid method for purification of epsilon-subunit, coupling factor 6, subunit d, and subunit e from rat liver H(+)-ATP synthase and determination of the complete amino acid sequence of epsilon-subunit.
Higuti T, Yoshihara Y, Kuroiwa K, Kawamura Y, Toda H, Sakiyama F
The Journal of biological chemistry267(31)22658-61(1992 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Atp5me gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp5me cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12985	NM_080481.1 Latest version!	Rattus norvegicus ATP synthase membrane subunit e (Atp5me), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp5me ( NM_080481.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_536729.1 Rattus norvegicus ATP synthase membrane subunit e (Atp5me), mRNA.

Price & Availability↑

CloneID	ORa12985
Clone ID Related Accession (Same CDS sequence)	NM_080481.1
Accession Version	NM_080481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Rattus norvegicus(Norway rat)
Product	ATP synthase subunit e, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D13121.1. ##Evidence-Data-START## Transcript exon combination :: D13121.1, CF977223.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGTGCCCC CGGTTCAGGT CTCTCCGCTC ATCAAGTTCG GCCGGTACTC CGCTCTGATC 
CTCGGCATGG CATACGGCGC CAAGCGCTAC AGTTACTTAA AACCCCGGGC AGAGGAGGAG 
AGAAGAATAG CAGCGGAGGA AAAAAAGAGA CTAGATGAGT TGAAACGGAT TGAGAGAGAA 
CTGGCAGAAG CTGAAGATGT CAGCATTTTC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536729.1
CDS	16..231
Translation	MVPPVQVSPLIKFGRYSALILGMAYGAKRYSYLKPRAEEERRIAAEEKKRLDELKRIERELAEAEDVSIFK

Target ORF information:

RefSeq Version	NM_080481.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP synthase membrane subunit e (Atp5me), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080481.1

ORF Insert Sequence:

ATGGTGCCCC CGGTTCAGGT CTCTCCGCTC ATCAAGTTCG GCCGGTACTC CGCTCTGATC 
CTCGGCATGG CATACGGCGC CAAGCGCTAC AGTTACTTAA AACCCCGGGC AGAGGAGGAG 
AGAAGAATAG CAGCGGAGGA AAAAAAGAGA CTAGATGAGT TGAAACGGAT TGAGAGAGAA 
CTGGCAGAAG CTGAAGATGT CAGCATTTTC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of two proteins associated with mammalian ATP synthase.
Molecular & cellular proteomics : MCP6(10)1690-9(2007 Oct)
Meyer B,Wittig I,Trifilieff E,Karas M,Schägger H

A functionally active human F1F0 ATPase can be purified by immunocapture from heart tissue and fibroblast cell lines. Subunit structure and activity studies.
The Journal of biological chemistry277(37)33906-12(2002 Sep)
Aggeler R,Coons J,Taylor SW,Ghosh SS,Garcia JJ,Capaldi RA,Marusich MF

Mitochondrial F(0)F(1) ATP synthase. Subunit regions on the F1 motor shielded by F(0), Functional significance, and evidence for an involvement of the unique F(0) subunit F(6).
The Journal of biological chemistry275(42)32931-9(2000 Oct)
Ko YH,Hullihen J,Hong S,Pedersen PL

Complete amino acid sequence of subunit e of rat liver mitochondrial H(+)-ATP synthase.
Biochemistry31(49)12451-4(1992 Dec)
Higuti T,Kuroiwa K,Kawamura Y,Yoshihara Y

A simple, rapid method for purification of epsilon-subunit, coupling factor 6, subunit d, and subunit e from rat liver H(+)-ATP synthase and determination of the complete amino acid sequence of epsilon-subunit.
The Journal of biological chemistry267(31)22658-61(1992 Nov)
Higuti T,Yoshihara Y,Kuroiwa K,Kawamura Y,Toda H,Sakiyama F