Lim2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lim2
Entrez Gene ID	114903
Full Name	lens intrinsic membrane protein 2
Synonyms	MP20,Mp19
General protein information	Preferred Names lens intrinsic membrane protein 2 Names lens fiber membrane intrinsic protein MP17 MP18 lens intrinsic membrane protein 2, 19kDa
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22
Summary	lens fiber-cell intrinsic membrane protein; ligand of galectin-3 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053771.1	NP_446223.1	lens fiber membrane intrinsic protein

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	LIM2	XP_001116349.1
Mus musculus (house mouse)	Lim2	NP_808361.1
Danio rerio (zebrafish)	lim2.5	NP_001013540.1
Homo sapiens (human)	LIM2	NP_085915.2
Pan troglodytes (chimpanzee)	LIM2	XP_001174186.1
Xenopus tropicalis (tropical clawed frog)	lim2	NP_001096249.1
Rattus norvegicus (Norway rat)	Lim2	NP_446223.1
Bos taurus (cattle)	LIM2	NP_776527.1
Canis lupus familiaris (dog)	LIM2	XP_003638853.1

Gene Ontology
Bibliography

Related articles in PubMed

A missense mutation in the LIM2 gene is associated with autosomal recessive presenile cataract in an inbred Iraqi Jewish family.
Pras E, Levy-Nissenbaum E, Bakhan T, Lahat H, Assia E, Geffen-Carmi N, Frydman M, Goldman B, Pras E
American journal of human genetics70(5)1363-7(2002 May)

MP20, the second most abundant lens membrane protein and member of the tetraspanin superfamily, joins the list of ligands of galectin-3.
Gonen T, Grey AC, Jacobs MD, Donaldson PJ, Kistler J
BMC cell biology217(2001)

Identification of a mutation in the MP19 gene, Lim2, in the cataractous mouse mutant To3.
Steele EC Jr, Kerscher S, Lyon MF, Glenister PH, Favor J, Wang J, Church RL
Molecular vision35(1997 May)

Two new cataract loci, Ccw and To3, and further mapping of the Npp and Opj cataracts in the mouse.
Kerscher S, Glenister PH, Favor J, Lyon MF
Genomics36(1)17-21(1996 Aug)

The human lens fiber-cell intrinsic membrane protein MP19 gene: isolation and sequence analysis.
Church RL, Wang JH
Current eye research12(12)1057-65(1993 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lim2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lim2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10584	NM_053771.1 Latest version!	Rattus norvegicus lens intrinsic membrane protein 2 (Lim2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lim2 ( NM_053771.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446223.1 Rattus norvegicus lens intrinsic membrane protein 2 (Lim2), mRNA.

Price & Availability↑

CloneID	ORa10584
Clone ID Related Accession (Same CDS sequence)	NM_053771.1
Accession Version	NM_053771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	lens fiber membrane intrinsic protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M87053.1. ##Evidence-Data-START## Transcript exon combination :: M87053.1, L04191.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTACAGCT TCATGGGTGG CGGCCTCTTC TGTGCCTGGG TGGGGACCAT CCTGTTGGTG 
GTAGCCACAG CGACGGACCA TTGGATGCAA TACCGGCTGT CAGGGTCCTT TGCACACCAG 
GGCCTGTGGC GTTACTGCCT GGGCAACAAG TGCTTCCTGC AGACAGAGAG CATCGCATAC 
TGGAATGCCA CGCGGGCTTT CATGATCCTG TCTGCCCTGT GTGCCACCTC GGGCATCATC 
ATGGGTGTCC TGGCCTTTGC GCAGCAATCC ACCTTCACCC GCCTCTCCAG GCCCTTCTCT 
GCTGGCATCA TGTTTTTTGC CTCCACCCTT TTCGTCCTGT TGGCCTTGGC CATTTACACC 
GGAGTCACCG TTAGTTTCCT CGGCCGCCGC TTTGGGGACT GGCGCTTTTC ATGGTCTTAC 
ATCCTGGGCT GGGTGGCCCT GCTCATGACC TTCTTTGCAG GAATTTTCTA CATGTGTGCC 
TACCGGATGC ATGAGTGCCG GCGCCTATCC ACCCCACGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446223.1
CDS	46..567
Translation	MYSFMGGGLFCAWVGTILLVVATATDHWMQYRLSGSFAHQGLWRYCLGNKCFLQTESIAYWNATRAFMILSALCATSGIIMGVLAFAQQSTFTRLSRPFSAGIMFFASTLFVLLALAIYTGVTVSFLGRRFGDWRFSWSYILGWVALLMTFFAGIFYMCAYRMHECRRLSTPR

Target ORF information:

RefSeq Version	NM_053771.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lens intrinsic membrane protein 2 (Lim2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053771.1

ORF Insert Sequence:

ATGTACAGCT TCATGGGTGG CGGCCTCTTC TGTGCCTGGG TGGGGACCAT CCTGTTGGTG 
GTAGCCACAG CGACGGACCA TTGGATGCAA TACCGGCTGT CAGGGTCCTT TGCACACCAG 
GGCCTGTGGC GTTACTGCCT GGGCAACAAG TGCTTCCTGC AGACAGAGAG CATCGCATAC 
TGGAATGCCA CGCGGGCTTT CATGATCCTG TCTGCCCTGT GTGCCACCTC GGGCATCATC 
ATGGGTGTCC TGGCCTTTGC GCAGCAATCC ACCTTCACCC GCCTCTCCAG GCCCTTCTCT 
GCTGGCATCA TGTTTTTTGC CTCCACCCTT TTCGTCCTGT TGGCCTTGGC CATTTACACC 
GGAGTCACCG TTAGTTTCCT CGGCCGCCGC TTTGGGGACT GGCGCTTTTC ATGGTCTTAC 
ATCCTGGGCT GGGTGGCCCT GCTCATGACC TTCTTTGCAG GAATTTTCTA CATGTGTGCC 
TACCGGATGC ATGAGTGCCG GCGCCTATCC ACCCCACGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A missense mutation in the LIM2 gene is associated with autosomal recessive presenile cataract in an inbred Iraqi Jewish family.
American journal of human genetics70(5)1363-7(2002 May)
Pras E,Levy-Nissenbaum E,Bakhan T,Lahat H,Assia E,Geffen-Carmi N,Frydman M,Goldman B,Pras E

MP20, the second most abundant lens membrane protein and member of the tetraspanin superfamily, joins the list of ligands of galectin-3.
BMC cell biology217(2001)
Gonen T,Grey AC,Jacobs MD,Donaldson PJ,Kistler J

Identification of a mutation in the MP19 gene, Lim2, in the cataractous mouse mutant To3.
Molecular vision35(1997 May)
Steele EC Jr,Kerscher S,Lyon MF,Glenister PH,Favor J,Wang J,Church RL

Two new cataract loci, Ccw and To3, and further mapping of the Npp and Opj cataracts in the mouse.
Genomics36(1)17-21(1996 Aug)
Kerscher S,Glenister PH,Favor J,Lyon MF

The human lens fiber-cell intrinsic membrane protein MP19 gene: isolation and sequence analysis.
Current eye research12(12)1057-65(1993 Dec)
Church RL,Wang JH

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Lim2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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