Plce1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plce1
Entrez Gene ID	114633
Full Name	phospholipase C, epsilon 1
Synonyms	Plce
General protein information	Preferred Names phospholipase C, epsilon 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 PLC-epsilon-1 phosphoinositide phospholipase C-epsilon-1 phosphoinositide-specific phospholipase C epsilon-1 phospholipase C epsilon
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q53
Summary	one of four classes of phospholipase C; directly regulated by Ras [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017588656.1	XP_017444145.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
XM_017588657.1	XP_017444146.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
XM_017588659.1	XP_017444148.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
NM_053758.2	NP_446210.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
XM_017588660.1	XP_017444149.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
XM_017588658.1	XP_017444147.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PLCE1	NP_057425.3
Gallus gallus (chicken)	LOC425224	XP_004942210.1
Danio rerio (zebrafish)	plce1	NP_001155125.1
Bos taurus (cattle)	PLCE1	NP_001192781.1
Macaca mulatta (Rhesus monkey)	PLCE1	XP_002805823.1
Mus musculus (house mouse)	Plce1	NP_062534.2
Rattus norvegicus (Norway rat)	Plce1	NP_446210.1
Caenorhabditis elegans (roundworm)	plc-1	NP_001123141.1
Canis lupus familiaris (dog)	PLCE1	NP_001130037.1
Pan troglodytes (chimpanzee)	PLCE1	XP_001149565.1
Xenopus tropicalis (tropical clawed frog)	plce1	XP_002937222.2

Gene Ontology
Bibliography

Related articles in PubMed

Compartmentalized cyclic nucleotides have opposing effects on regulation of hypertrophic phospholipase Cε signaling in cardiac myocytes.
Nash CA, Brown LM, Malik S, Cheng X, Smrcka AV
Journal of molecular and cellular cardiology12151-59(2018 08)

G protein βγ subunits directly interact with and activate phospholipase Cϵ.
Madukwe JC, Garland-Kuntz EE, Lyon AM, Smrcka AV
The Journal of biological chemistry293(17)6387-6397(2018 04)

Phospholipase Cε Modulates Rap1 Activity and the Endothelial Barrier.
DiStefano PV, Smrcka AV, Glading AJ
PloS one11(9)e0162338(2016)

Phospholipase C epsilon scaffolds to muscle-specific A kinase anchoring protein (mAKAPbeta) and integrates multiple hypertrophic stimuli in cardiac myocytes.
Zhang L, Malik S, Kelley GG, Kapiloff MS, Smrcka AV
The Journal of biological chemistry286(26)23012-21(2011 Jul)

Mutational analysis of the PLCE1 gene in steroid resistant nephrotic syndrome.
Boyer O, Benoit G, Gribouval O, Nevo F, Pawtowski A, Bilge I, Bircan Z, Deschênes G, Guay-Woodford LM, Hall M, Macher MA, Soulami K, Stefanidis CJ, Weiss R, Loirat C, Gubler MC, Antignac C
Journal of medical genetics47(7)445-52(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Plce1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plce1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa56297	XM_017588660.1 Latest version!	Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa10541	NM_053758.2 Latest version!	Rattus norvegicus phospholipase C, epsilon 1 (Plce1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa56295	XM_017588658.1 Latest version!	Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa56295	XM_017588657.1 Latest version!	Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa56295	XM_017588656.1 Latest version!	Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa56296	XM_017588659.1 Latest version!	Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plce1 ( XM_017588660.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444149.1 Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa56297
Clone ID Related Accession (Same CDS sequence)	XM_017588660.1
Accession Version	XM_017588660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCAG AAAGAAGAAC AGCAGGAAGA GGCCTTGCAG TGATGGAAGA GATCTACAGT 
GCCGTGAGAA GAGAAGAGAC AGAAGATACG GTTGGCTCTC TTCTGCATTG CTCCACACAG 
CTCCCAACCC CTGACACAGC CCATGGAAGG ATCGGAGATG GTCCCTGCCT AAAGCAATGT 
GTGCGAGACA GCGAGTGTGA GTACCGTGCC ACCCTCCAAA GGACGTCCAT AGCTCAGTAC 
ATCACCGGTT CTCTCCTAGA GGCAACCACA TCTTTAGGAG CAAGAAGCAG CCTTCTCAGT 
TCCTTTGGAG GATCCACTGG GCGAATAATG CTGAAAGAAC GCCAACCGGG CACTTCTATG 
GCCAATTCCA GTCCTGTTCC TTCAAGTTCA GCTGGAATCA GCAAGGAGCT GATTGATCTC 
CAGCCCCTCA TTCAGTTCCC GGAGGAAGTA GCCAGCATCT TAACAGAACA GGAACAGAAC 
ATTTACCGCA GGGTCCTGCC CATGGACTAC CTCTGTTTCT TAACCCGGGA CTTGAGTTCC 
CCCGAGTGTC AGAGATCACT GCCCCGTCTC AAAGCATGCA TCTCAGAATC AATCCTCATG 
TCTCAAAGTG GAGAGCACAA TGCCCTTGAA GACCTCGTGA TGAGATTTAA CGAGGTGAGC 
TCCTGGGTAA CATGGCTGAT CCTCACGGCA GGCTCCATGG AGGAGAAGCG GGAAGTCTTT 
TCTTATTTGG TGCATGTGGC CAAATGCTGC TGGAACATGG GCAACTACAA TGCTGTCATG 
GAGTTCCTGG CTGGCCTCAG GTCAAGGAAA GTTCTGAAGA TGTGGCAGTT CATGGACCAG 
TCTGACATCG AGACCATGAG GAGCCTGAAA GACGCCATGG CTCAGCATGA GTCTTCCGTG 
GAGTACAAGA AGGTAGTGAC CCGAGCCTTG CACATCCCCG GCTGCAAAGT GGTTCCTTTC 
TGTGGGGTGT TCCTGAAGGA GCTCTGTGAG GTGCTTGATG GTGCCTCTGG CCTCCTGAAG 
CTCTGCCCAC GATACAGCTC CCAAGAAGAA GCCCTGGAGT TTGTAGCGGA TTACAGTGGA 
CAAGATAACT TCTTACAGCG AGTGGGGCAG AATGGCTTGA AGAATCCAGA GAAGGAGCTC 
ACGGTCAACA GCATCTTTCA GATCATCCGG AGCTGCAGCC GAAGCTTGGA GACGGAGGAC 
GAGGAGAGCG CCAGCGAAGG GAGCGGCTCT AGGAAAAACT CCCTCAAGGA CAAAACCCGG 
TGGCAGTTTA TAATTGGAGA TTTGCTGGAT TCAGACAATG ACATCTTTGA GAAGTCCAAA 
GAGTGCGACC CTCATGGATC AGAGGAGTCA CAGAAAGCCT TTGACCATGG GACAGAGCTC 
ATCCCATGGT ATGTGCTGTC CATCCAAGCG GATGTGCACC AGTTCCTCCT GCAGGGGGCC 
ACAGTCATCC ACTATGACCA GGACACACAT CTCTCCGCCC GCTGCTTTCT CCAGTTGCAG 
CCTGACAATA GTACCTTGAC CTGGATGAAA CCTCCCACTG CCTCCCCAGC TGGTGCCAGA 
CTCAAGCTCG GTGTGCTTAG TAACGTGGCC GAACCTGGCA AGTTCCCATC ACTGGGCAAC 
GCTGGGGTGA GCGGCCTGGT AGAGGGAATC CTGGATCTGT TTTCAGTGAA GGCTGTGTAC 
ATGGGACACC CTGGCATTGA TATACACACT GTGTGTGTCC AGAACAAACT TAGTAGCATG 
CTTCTGTCAG AGACTGGGGT GACGCTGCTC TATGGGCTTC AGACCACCGA CAATAGATTA 
CTGCACTTCG TGGCACCAAA GCACACAGCT AAAATGCTTT TCAGTGGATT GCTGGAACTC 
ACTACAGCTG TGAGAAAGAT ACGGAAGTTC CCTGACCAAA GACAACAGTG GCTTCGGAAA 
CAGTATGTCA GCTTCTATCA GGAGGATGGT CGGTATGAAG GCCCAACCTT GGCACATGCT 
GTGGAGTTGT TTGGTGGTAG GAGATGGAGC ACTAGAAACC CCAGCCCTGG GATGTCAGCA 
AAGAATGCCG AGAAGCCCAA CATGCAGAGA AACAACACCC TAGGCATAAG CACCACCAAG 
AAAAAGAAGA AAATGCTCAT GAGGGGGGAG AGTGGAGAGG TGACGGACGA TGAGATGGCC 
ACCCGCAAGG CCAAAATGTA CAGAGAGTGT CGCAGCCGGA GCGGCTCTGA TCCTCAAGAG 
GCTAATGAAC AGGAAGACTC AGAGGCCAAT GTCATCACTA ACCCTCCCAA CCCCCTCCAT 
TCCAGAAGAG CCTACTCATT GACCACGGCT GGGTCCCCCA ACTTGGCTAC CGGGATGTCA 
TCTCCTATCA GTGCCTGGAG TAGCAGCAGC TGGCACGGGC GGATCAAAGG AGGAATGAAA 
GGCTTCCAGA GCTTCATGGT CTCAGACAGT AACATGAGTT TCATTGAATT TGTCGAGCTG 
TTCAAATCAT TCAGCATAAG GAGCCGCAAG GACTTGAAGG ACATCTTTGA CATCTACTCT 
GTACCCTGCA ATCGGTCTGC TTCTGAGTCA GCCCCACTGT ACACCAACCT GACAATCGAA 
GAAAATACCA ATGACCTTCA GCCTGACCTA GATTTGCTGA CTAGAAACGT CTCCGACTTG 
GGGCTGTTCA TGAAGAGTAA GCAGCAGCTT TCTGACAACC AGAGGCAGAT ATCTGACGCC 
ATTGCTGCTG CAAGCATTGT GACTAATGGC ACTGGGATTG AAAGCACGTC CCTGGGCATA 
TTTGGGGTCG GCATACTCCA GCTCAACGAC TTCCTGGTGA ATTGCCAAGG AGAACACTGC 
ACATATGATG AAATCCTCAG CATCATCCAG AAGTTCGAGC CCAACATCAG CATGTGTCAT 
CAAGGTCTAT TGTCCTTCGA AGGGTTTGCA AGGTTTCTGA TGGATAAAGA CAATTTCGCC 
TCAAAAAATG ACGAGTCGCG GGAGAACAAG AAAGACCTGC AGCTACCCCT CTCCTACTAT 
TACATCGAAT CTTCGCACAA CACCTACCTC ACCGGCCATC AGCTCAAGGG GGAGTCCTCT 
GTGGAGCTCT ACAGCCAGGT CCTCTTGCAA GGATGCAGAA GCATAGAGCT GGACTGCTGG 
GATGGAGATG ATGGGATGCC CATTATTTAT CATGGACATA CGCTGACAAC CAAGATCCCC 
TTCAAGGAAG TGGTGGAAGC CATCGATCGC AGTGCCTTCA TCACCTCCGA CCTGCCAATC 
ATCATATCCA TTGAGAACCA TTGCTCATTG CCTCAGCAAC GGAAAATGGC AGAAATTTTC 
AAGAGTGTGT TTGGAGAAAA GCTGGTGGCT AAGTTCTTGT TCGAGACCGA TTTCTCAGAT 
GACCCAATGC TTCCCTCACC TGACCAACTT AGAAGGAAAG TGCTTCTTAA AAACAAGAAG 
CTAAAGGCCC ATCAGACACC TGTCGACATC CTCAAACAAA AGGCTCATCA GTTAGCATCC 
ATGCAAACAC AGGCTTTCAC TGGTGGGAAT GCCAACCCGC CACCTGCCAG TAATGAGGAA 
GAGGAAGACG AGGAGGACGA ATATGATTAT GATTACGAGT CCCTGTCTGA TGCAGATGTC 
CTTACTGCTT CCCCTGTTCC TAACAGTCAG GAAGACAACA TTCTGGAAGA CAGACCTGAG 
AACAAATCAT GTGCCGACAA GCTTCAGTTT GAGTACAATG AAGAAGTCCC CAAGAGGATA 
AAGAAAGCAG ACAACTCCTC CGGCAATAAG GGAAAGGTCT ATGACATGGA ACTGGGAGAG 
GAGTTTTACC TTCCTCAGAA CAAAAAAGAA AGCAGGCAGA TTGCACCGGA GCTCTCTGAC 
CTCGTCATCT ATTGCCAGGC GGTAAAATTT CCAGGCCTGT CAACTCTAAA TTCATCTGGC 
TCTGGCAGAG GGAAAGAAAG GAAAAGCAGG AAGTCCATTT TTGGCAACAA TCCCGGCAGA 
ATGAGCCCAG GGGAGACAGC GTCATTTAAC AGAACATCTG GAAAAAGTTC TTGTGAAGGA 
ATTCGACAAA TCTGGGAGGA ACCTCCACTC AGCCCAAACA CATCCCTCAG CGCCATCATT 
AGAACTCCTA AATGCTATCA TATCTCGTCA CTGAATGAAA ATGCCGCCAA ACGTCTGTGT 
CGCAGGTATT CTCAGAAACT GATCCAGCAC ACTGCTTGCC AGCTGCTGAG GACCTACCCG 
GCAGCGACCA GAATCGACTC ATCCAATCCC AACCCCCTCA TGTTCTGGCT CCATGGGATA 
CAGCTTGTAG CTCTAAACTA CCAGACGGAT GACCTTCCTT TACATTTAAA CGCTGCCATG 
TTTGAGGCGA ATGGTGGGTG TGGCTATGTT TTGAAGCCCC CTGTCCTGTG GGATAAAAGC 
TGTCCCATGT ATCAGAAGTT TTCACCACTG GAAAGAGACC TGGACGCCAT GGATCCTGCT 
ACCTATTCTT TAACCATCAT CTCTGGCCAG AATGTGTGTC CTAGTAACAG CACCGGAAGT 
CCGTGCATTG AAGTTGACGT CTTGGGCATG CCCCTGGACA GCTGCCATTT CCGCACAAAA 
CCCATCCACC GAAACACTCT GAACCCCATG TGGAATGAGC AGTTTCTCTT CCGGGTTCAC 
TTTGAAGATC TTGTATTTCT TCGTTTTGCA GTTGTGGAGA ACAACAGTTC GGCGATAACG 
GCTCAGAGAA TCATTCCACT CAAAGCTTTA AAGAGAGGAT ATCGGCATCT TCAGCTGCGG 
AACCTCCACA ACGAGATCTT GGAAATCTCT AGCTTATTCA TAAACAGCAG GAGGATGGAA 
GATAATCCCT CTGGCAGTAC CCGGCCAGCC TCTTTGATGT TCAATACTGA AGAGAGGAAA 
TGTTCTCAGA CTCACAAAGT CACAGTGCAT GGTGTCCCAG GTCCAGAGCC CTTTGCTGTT 
TTCACAATAA ACGAAGGCAC CAAGGCAAAG CAGCTTCTCC AACAGATTTT GGCAGTTGAC 
CAGGACACCA AACTTACAGC CGCAGACTAT TTTCTGATGG AAGAGAAGCA TTTTATATCT 
AAGGAAAAGA ATGAATGCAG GAAACAACCA TTCCAGAGAG CTGTGGGCCC AGAGGAAGAT 
ATTGTGCAGA TTTTAAACAG CTGGTTCCCA GAAGAAGGCT ACGTAGGCAG GATTGTCTTA 
AAGCCCCAAC AAGAAACCCT AGAAGAGAAA AACATTGTCC ACGATGACAG AGAGGTGATC 
TTGAGCTCAG AAGAGGAGAG TTTCTTTGTC CAAGTGCACG ATGTCTCTCC AGAGCAGCCT 
CGAACTGTCA TCAAAGCCCC ACGTGTCAGC ACTGCACAGG ATGTGATTCA GCAGACCTTG 
TGCAAAGCCA AATATTCCTA CAGCATCCTG AACAACCCCA ATCCGTGTGA CTATGTGCTT 
TTGGAAGAGG TAATGAAAGA TGCACCCAAT AAGAAGTCTT CTACCCCGAA GTCCTCACAG 
AGGATCCTCT TGGACCAAGA ATGTGTGTTT CAAGCCCAAA GCAAGTGGAA GGGTGCAGGG 
AAATTCATCC TTAAGCTAAA GGAGCAGGTG CAGGCATCCA GAGAAGATAA AAGGAGGGGC 
ATCTCCTTTG CAAGTGAACT CAAGAAACTC ACGAAGTCCA CTAAGCAAAC CCGAGGACTC 
ACCTCGCCTC CTCAGCTTGT GGCCTCAGAA AGTGTGCAAA GCAAGGAGGA GAAACCCATG 
GGTGCCTTGG CCTCTGGTGA CACAGCGGGC TACCAGTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444149.1
CDS	4009..9750
Translation	MVSERRTAGRGLAVMEEIYSAVRREETEDTVGSLLHCSTQLPTPDTAHGRIGDGPCLKQCVRDSECEYRATLQRTSIAQYITGSLLEATTSLGARSSLLSSFGGSTGRIMLKERQPGTSMANSSPVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRRVLPMDYLCFLTRDLSSPECQRSLPRLKACISESILMSQSGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSVEYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEEALEFVADYSGQDNFLQRVGQNGLKNPEKELTVNSIFQIIRSCSRSLETEDEESASEGSGSRKNSLKDKTRWQFIIGDLLDSDNDIFEKSKECDPHGSEESQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTASPAGARLKLGVLSNVAEPGKFPSLGNAGVSGLVEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSMLLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTTAVRKIRKFPDQRQQWLRKQYVSFYQEDGRYEGPTLAHAVELFGGRRWSTRNPSPGMSAKNAEKPNMQRNNTLGISTTKKKKKMLMRGESGEVTDDEMATRKAKMYRECRSRSGSDPQEANEQEDSEANVITNPPNPLHSRRAYSLTTAGSPNLATGMSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFIEFVELFKSFSIRSRKDLKDIFDIYSVPCNRSASESAPLYTNLTIEENTNDLQPDLDLLTRNVSDLGLFMKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPNISMCHQGLLSFEGFARFLMDKDNFASKNDESRENKKDLQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQLASMQTQAFTGGNANPPPASNEEEEDEEDEYDYDYESLSDADVLTASPVPNSQEDNILEDRPENKSCADKLQFEYNEEVPKRIKKADNSSGNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNSSGSGRGKERKSRKSIFGNNPGRMSPGETASFNRTSGKSSCEGIRQIWEEPPLSPNTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERDLDAMDPATYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLKALKRGYRHLQLRNLHNEILEISSLFINSRRMEDNPSGSTRPASLMFNTEERKCSQTHKVTVHGVPGPEPFAVFTINEGTKAKQLLQQILAVDQDTKLTAADYFLMEEKHFISKEKNECRKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKNIVHDDREVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPCDYVLLEEVMKDAPNKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRRGISFASELKKLTKSTKQTRGLTSPPQLVASESVQSKEEKPMGALASGDTAGYQS

Target ORF information:

RefSeq Version	XM_017588660.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588660.1

ORF Insert Sequence:

ATGGTTTCAG AAAGAAGAAC AGCAGGAAGA GGCCTTGCAG TGATGGAAGA GATCTACAGT 
GCCGTGAGAA GAGAAGAGAC AGAAGATACG GTTGGCTCTC TTCTGCATTG CTCCACACAG 
CTCCCAACCC CTGACACAGC CCATGGAAGG ATCGGAGATG GTCCCTGCCT AAAGCAATGT 
GTGCGAGACA GCGAGTGTGA GTACCGTGCC ACCCTCCAAA GGACGTCCAT AGCTCAGTAC 
ATCACCGGTT CTCTCCTAGA GGCAACCACA TCTTTAGGAG CAAGAAGCAG CCTTCTCAGT 
TCCTTTGGAG GATCCACTGG GCGAATAATG CTGAAAGAAC GCCAACCGGG CACTTCTATG 
GCCAATTCCA GTCCTGTTCC TTCAAGTTCA GCTGGAATCA GCAAGGAGCT GATTGATCTC 
CAGCCCCTCA TTCAGTTCCC GGAGGAAGTA GCCAGCATCT TAACAGAACA GGAACAGAAC 
ATTTACCGCA GGGTCCTGCC CATGGACTAC CTCTGTTTCT TAACCCGGGA CTTGAGTTCC 
CCCGAGTGTC AGAGATCACT GCCCCGTCTC AAAGCATGCA TCTCAGAATC AATCCTCATG 
TCTCAAAGTG GAGAGCACAA TGCCCTTGAA GACCTCGTGA TGAGATTTAA CGAGGTGAGC 
TCCTGGGTAA CATGGCTGAT CCTCACGGCA GGCTCCATGG AGGAGAAGCG GGAAGTCTTT 
TCTTATTTGG TGCATGTGGC CAAATGCTGC TGGAACATGG GCAACTACAA TGCTGTCATG 
GAGTTCCTGG CTGGCCTCAG GTCAAGGAAA GTTCTGAAGA TGTGGCAGTT CATGGACCAG 
TCTGACATCG AGACCATGAG GAGCCTGAAA GACGCCATGG CTCAGCATGA GTCTTCCGTG 
GAGTACAAGA AGGTAGTGAC CCGAGCCTTG CACATCCCCG GCTGCAAAGT GGTTCCTTTC 
TGTGGGGTGT TCCTGAAGGA GCTCTGTGAG GTGCTTGATG GTGCCTCTGG CCTCCTGAAG 
CTCTGCCCAC GATACAGCTC CCAAGAAGAA GCCCTGGAGT TTGTAGCGGA TTACAGTGGA 
CAAGATAACT TCTTACAGCG AGTGGGGCAG AATGGCTTGA AGAATCCAGA GAAGGAGCTC 
ACGGTCAACA GCATCTTTCA GATCATCCGG AGCTGCAGCC GAAGCTTGGA GACGGAGGAC 
GAGGAGAGCG CCAGCGAAGG GAGCGGCTCT AGGAAAAACT CCCTCAAGGA CAAAACCCGG 
TGGCAGTTTA TAATTGGAGA TTTGCTGGAT TCAGACAATG ACATCTTTGA GAAGTCCAAA 
GAGTGCGACC CTCATGGATC AGAGGAGTCA CAGAAAGCCT TTGACCATGG GACAGAGCTC 
ATCCCATGGT ATGTGCTGTC CATCCAAGCG GATGTGCACC AGTTCCTCCT GCAGGGGGCC 
ACAGTCATCC ACTATGACCA GGACACACAT CTCTCCGCCC GCTGCTTTCT CCAGTTGCAG 
CCTGACAATA GTACCTTGAC CTGGATGAAA CCTCCCACTG CCTCCCCAGC TGGTGCCAGA 
CTCAAGCTCG GTGTGCTTAG TAACGTGGCC GAACCTGGCA AGTTCCCATC ACTGGGCAAC 
GCTGGGGTGA GCGGCCTGGT AGAGGGAATC CTGGATCTGT TTTCAGTGAA GGCTGTGTAC 
ATGGGACACC CTGGCATTGA TATACACACT GTGTGTGTCC AGAACAAACT TAGTAGCATG 
CTTCTGTCAG AGACTGGGGT GACGCTGCTC TATGGGCTTC AGACCACCGA CAATAGATTA 
CTGCACTTCG TGGCACCAAA GCACACAGCT AAAATGCTTT TCAGTGGATT GCTGGAACTC 
ACTACAGCTG TGAGAAAGAT ACGGAAGTTC CCTGACCAAA GACAACAGTG GCTTCGGAAA 
CAGTATGTCA GCTTCTATCA GGAGGATGGT CGGTATGAAG GCCCAACCTT GGCACATGCT 
GTGGAGTTGT TTGGTGGTAG GAGATGGAGC ACTAGAAACC CCAGCCCTGG GATGTCAGCA 
AAGAATGCCG AGAAGCCCAA CATGCAGAGA AACAACACCC TAGGCATAAG CACCACCAAG 
AAAAAGAAGA AAATGCTCAT GAGGGGGGAG AGTGGAGAGG TGACGGACGA TGAGATGGCC 
ACCCGCAAGG CCAAAATGTA CAGAGAGTGT CGCAGCCGGA GCGGCTCTGA TCCTCAAGAG 
GCTAATGAAC AGGAAGACTC AGAGGCCAAT GTCATCACTA ACCCTCCCAA CCCCCTCCAT 
TCCAGAAGAG CCTACTCATT GACCACGGCT GGGTCCCCCA ACTTGGCTAC CGGGATGTCA 
TCTCCTATCA GTGCCTGGAG TAGCAGCAGC TGGCACGGGC GGATCAAAGG AGGAATGAAA 
GGCTTCCAGA GCTTCATGGT CTCAGACAGT AACATGAGTT TCATTGAATT TGTCGAGCTG 
TTCAAATCAT TCAGCATAAG GAGCCGCAAG GACTTGAAGG ACATCTTTGA CATCTACTCT 
GTACCCTGCA ATCGGTCTGC TTCTGAGTCA GCCCCACTGT ACACCAACCT GACAATCGAA 
GAAAATACCA ATGACCTTCA GCCTGACCTA GATTTGCTGA CTAGAAACGT CTCCGACTTG 
GGGCTGTTCA TGAAGAGTAA GCAGCAGCTT TCTGACAACC AGAGGCAGAT ATCTGACGCC 
ATTGCTGCTG CAAGCATTGT GACTAATGGC ACTGGGATTG AAAGCACGTC CCTGGGCATA 
TTTGGGGTCG GCATACTCCA GCTCAACGAC TTCCTGGTGA ATTGCCAAGG AGAACACTGC 
ACATATGATG AAATCCTCAG CATCATCCAG AAGTTCGAGC CCAACATCAG CATGTGTCAT 
CAAGGTCTAT TGTCCTTCGA AGGGTTTGCA AGGTTTCTGA TGGATAAAGA CAATTTCGCC 
TCAAAAAATG ACGAGTCGCG GGAGAACAAG AAAGACCTGC AGCTACCCCT CTCCTACTAT 
TACATCGAAT CTTCGCACAA CACCTACCTC ACCGGCCATC AGCTCAAGGG GGAGTCCTCT 
GTGGAGCTCT ACAGCCAGGT CCTCTTGCAA GGATGCAGAA GCATAGAGCT GGACTGCTGG 
GATGGAGATG ATGGGATGCC CATTATTTAT CATGGACATA CGCTGACAAC CAAGATCCCC 
TTCAAGGAAG TGGTGGAAGC CATCGATCGC AGTGCCTTCA TCACCTCCGA CCTGCCAATC 
ATCATATCCA TTGAGAACCA TTGCTCATTG CCTCAGCAAC GGAAAATGGC AGAAATTTTC 
AAGAGTGTGT TTGGAGAAAA GCTGGTGGCT AAGTTCTTGT TCGAGACCGA TTTCTCAGAT 
GACCCAATGC TTCCCTCACC TGACCAACTT AGAAGGAAAG TGCTTCTTAA AAACAAGAAG 
CTAAAGGCCC ATCAGACACC TGTCGACATC CTCAAACAAA AGGCTCATCA GTTAGCATCC 
ATGCAAACAC AGGCTTTCAC TGGTGGGAAT GCCAACCCGC CACCTGCCAG TAATGAGGAA 
GAGGAAGACG AGGAGGACGA ATATGATTAT GATTACGAGT CCCTGTCTGA TGCAGATGTC 
CTTACTGCTT CCCCTGTTCC TAACAGTCAG GAAGACAACA TTCTGGAAGA CAGACCTGAG 
AACAAATCAT GTGCCGACAA GCTTCAGTTT GAGTACAATG AAGAAGTCCC CAAGAGGATA 
AAGAAAGCAG ACAACTCCTC CGGCAATAAG GGAAAGGTCT ATGACATGGA ACTGGGAGAG 
GAGTTTTACC TTCCTCAGAA CAAAAAAGAA AGCAGGCAGA TTGCACCGGA GCTCTCTGAC 
CTCGTCATCT ATTGCCAGGC GGTAAAATTT CCAGGCCTGT CAACTCTAAA TTCATCTGGC 
TCTGGCAGAG GGAAAGAAAG GAAAAGCAGG AAGTCCATTT TTGGCAACAA TCCCGGCAGA 
ATGAGCCCAG GGGAGACAGC GTCATTTAAC AGAACATCTG GAAAAAGTTC TTGTGAAGGA 
ATTCGACAAA TCTGGGAGGA ACCTCCACTC AGCCCAAACA CATCCCTCAG CGCCATCATT 
AGAACTCCTA AATGCTATCA TATCTCGTCA CTGAATGAAA ATGCCGCCAA ACGTCTGTGT 
CGCAGGTATT CTCAGAAACT GATCCAGCAC ACTGCTTGCC AGCTGCTGAG GACCTACCCG 
GCAGCGACCA GAATCGACTC ATCCAATCCC AACCCCCTCA TGTTCTGGCT CCATGGGATA 
CAGCTTGTAG CTCTAAACTA CCAGACGGAT GACCTTCCTT TACATTTAAA CGCTGCCATG 
TTTGAGGCGA ATGGTGGGTG TGGCTATGTT TTGAAGCCCC CTGTCCTGTG GGATAAAAGC 
TGTCCCATGT ATCAGAAGTT TTCACCACTG GAAAGAGACC TGGACGCCAT GGATCCTGCT 
ACCTATTCTT TAACCATCAT CTCTGGCCAG AATGTGTGTC CTAGTAACAG CACCGGAAGT 
CCGTGCATTG AAGTTGACGT CTTGGGCATG CCCCTGGACA GCTGCCATTT CCGCACAAAA 
CCCATCCACC GAAACACTCT GAACCCCATG TGGAATGAGC AGTTTCTCTT CCGGGTTCAC 
TTTGAAGATC TTGTATTTCT TCGTTTTGCA GTTGTGGAGA ACAACAGTTC GGCGATAACG 
GCTCAGAGAA TCATTCCACT CAAAGCTTTA AAGAGAGGAT ATCGGCATCT TCAGCTGCGG 
AACCTCCACA ACGAGATCTT GGAAATCTCT AGCTTATTCA TAAACAGCAG GAGGATGGAA 
GATAATCCCT CTGGCAGTAC CCGGCCAGCC TCTTTGATGT TCAATACTGA AGAGAGGAAA 
TGTTCTCAGA CTCACAAAGT CACAGTGCAT GGTGTCCCAG GTCCAGAGCC CTTTGCTGTT 
TTCACAATAA ACGAAGGCAC CAAGGCAAAG CAGCTTCTCC AACAGATTTT GGCAGTTGAC 
CAGGACACCA AACTTACAGC CGCAGACTAT TTTCTGATGG AAGAGAAGCA TTTTATATCT 
AAGGAAAAGA ATGAATGCAG GAAACAACCA TTCCAGAGAG CTGTGGGCCC AGAGGAAGAT 
ATTGTGCAGA TTTTAAACAG CTGGTTCCCA GAAGAAGGCT ACGTAGGCAG GATTGTCTTA 
AAGCCCCAAC AAGAAACCCT AGAAGAGAAA AACATTGTCC ACGATGACAG AGAGGTGATC 
TTGAGCTCAG AAGAGGAGAG TTTCTTTGTC CAAGTGCACG ATGTCTCTCC AGAGCAGCCT 
CGAACTGTCA TCAAAGCCCC ACGTGTCAGC ACTGCACAGG ATGTGATTCA GCAGACCTTG 
TGCAAAGCCA AATATTCCTA CAGCATCCTG AACAACCCCA ATCCGTGTGA CTATGTGCTT 
TTGGAAGAGG TAATGAAAGA TGCACCCAAT AAGAAGTCTT CTACCCCGAA GTCCTCACAG 
AGGATCCTCT TGGACCAAGA ATGTGTGTTT CAAGCCCAAA GCAAGTGGAA GGGTGCAGGG 
AAATTCATCC TTAAGCTAAA GGAGCAGGTG CAGGCATCCA GAGAAGATAA AAGGAGGGGC 
ATCTCCTTTG CAAGTGAACT CAAGAAACTC ACGAAGTCCA CTAAGCAAAC CCGAGGACTC 
ACCTCGCCTC CTCAGCTTGT GGCCTCAGAA AGTGTGCAAA GCAAGGAGGA GAAACCCATG 
GGTGCCTTGG CCTCTGGTGA CACAGCGGGC TACCAGTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plce1 ( NM_053758.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446210.1 Rattus norvegicus phospholipase C, epsilon 1 (Plce1), mRNA.

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CloneID	ORa10541
Clone ID Related Accession (Same CDS sequence)	NM_053758.2
Accession Version	NM_053758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568476800000
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF323615.1. On Aug 30, 2012 this sequence version replaced NM_053758.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF323615.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACGTCCG AAGAAATGGC CGCTTCCTTC CTCATCCCGG TGCCTCAAAG GAAAGTGGCT 
TCTGCCCAGT CAGTGGCAGA GGAGCGGGGT GAAAAGGTCT CAGAAGCCGG CATTCCTAAG 
ACACGAGCTG GCAGACAGGG TGGGCTCACC CCTCGTACCA TCTCACAGCG GAACGAACCT 
GAGGAAGAGT CTCCTAGAAC TGATTTTTCC CAGGTCTTCT CAATAGCGAG GGGGGAACTA 
GACAGCGACG AGAATCACAA TGAAAGGTGC TGGGAGGAAA ATGTGCCAGG TTCCACGAAA 
AACCACGCGG TTAACTGCAA CAGCTTATTG CAAAGCCACC AGCATGCGCT TCCCCCGAGC 
CAGCTCTGTG AAGTCTGTGA CTCTGTCACA GAAGAACACC TGTGTTTGCA GCCTGGGATT 
CCTTCTCCAC TGGAAAGGAA GGTGTTTCCT GGGATCGAAC TGGAGATGGA GGACTCTCCC 
ATGGACGTGA GCCCTTTGGG AAACCAGCCT GGGATCATGG AGTCCAGCGG ACCTCACTCT 
GACCGCAACA TGGCGGTATT TCATTTCCAT TATGCAGGTG ACAGGACAAT GCCAGGTGCT 
TTCCATACCC TGTCAGAAAA ATTCATTTTG GATGACTGTG CAAACTGTGT TACTCTTCCT 
GGGGGGCAGC AAAACAAAAA TTACATGGCG TATACTTGCA AACTGGTGGA GTTGACAAGA 
ACCTGTGGTA GTAAGAATGG GCAGCTGAAG TGTGACCACT GCACCTCGCT GCGGGATGAA 
TACCTGTGCT TTGAAAGCTC CTGCCGGAAG GCTGAGGCTC TCTCCTCAGG TGGTGGCTTT 
TGCGAGGACG GATTTACTCA TGGTCCCTCT GCCAAGACTT TTCTGAACCC TTTGGAGGAA 
TTCTCTGATA ATTGTGAAGA CGTGGACGAT ATTTTTAAAG GCAAAAAGGA GCGGTCCACT 
TTGTTAGTCC GAAGGTTTTG TAAAAATGAC AGGGAAGTTA AGAAGTCTGT GTATACTGGG 
ACAAGGGCCA TTGTGAGAAC CCTGCCTTCT GGCCACATTG GGCTGGCTGC TTGGAGTTAC 
GTCGATCAGA AGAAAGCTGG TCTCATGTGG CCTTGTGGGA ATGGAATGAG ACCTCTGTCC 
ACAGTGGACG TAAGGCAAAG TGGGAGACAG CGTCTGTCTG AAGCCCAGTG GTGTCTGATC 
TACAGTGCCG TGAGAAGAGA AGAGACAGAA GATACGGTTG GCTCTCTTCT GCATTGCTCC 
ACACAGCTCC CAACCCCTGA CACAGCCCAT GGAAGGATCG GAGATGGTCC CTGCCTAAAG 
CAATGTGTGC GAGACAGCGA GTGTGAGTAC CGTGCCACCC TCCAAAGGAC GTCCATAGCT 
CAGTACATCA CCGGTTCTCT CCTAGAGGCA ACCACATCTT TAGGAGCAAG AAGCAGCCTT 
CTCAGTTCCT TTGGAGGATC CACTGGGCGA ATAATGCTGA AAGAACGCCA ACCGGGCACT 
TCTATGGCCA ATTCCAGTCC TGTTCCTTCA AGTTCAGCTG GAATCAGCAA GGAGCTGATT 
GATCTCCAGC CCCTCATTCA GTTCCCGGAG GAAGTAGCCA GCATCTTAAC AGAACAGGAA 
CAGAACATTT ACCGCAGGGT CCTGCCCATG GACTACCTCT GTTTCTTAAC CCGGGACTTG 
AGTTCCCCCG AGTGTCAGAG ATCACTGCCC CGTCTCAAAG CATGCATCTC AGAATCAATC 
CTCATGTCTC AAAGTGGAGA GCACAATGCC CTTGAAGACC TCGTGATGAG ATTTAACGAG 
GTGAGCTCCT GGGTAACATG GCTGATCCTC ACGGCAGGCT CCATGGAGGA GAAGCGGGAA 
GTCTTTTCTT ATTTGGTGCA TGTGGCCAAA TGCTGCTGGA ACATGGGCAA CTACAATGCT 
GTCATGGAGT TCCTGGCTGG CCTCAGGTCA AGGAAAGTTC TGAAGATGTG GCAGTTCATG 
GACCAGTCTG ACATCGAGAC CATGAGGAGC CTGAAAGACG CCATGGCTCA GCATGAGTCT 
TCCGTGGAGT ACAAGAAGGT AGTGACCCGA GCCTTGCACA TCCCCGGCTG CAAAGTGGTT 
CCCTTCTGTG GGGTGTTCCT GAAGGAGCTC TGTGAGGTGC TTGATGGTGC CTCTGGCCTC 
CTGAAGCTCT GCCCACGATA CAGCTCCCAA GAAGAAGCCC TGGAGTTTGT AGCGGATTAC 
AGTGGACAAG ATAACTTCTT ACAGCGAGTG GGGCAGAATG GCTTGAAGAA TCCAGAGAAG 
GAGCTCACGG TCAACAGCAT CTTTCAGATC ATCCGGAGCT GCAGCCGAAG CTTGGAGACG 
GAGGACGAGG AGAGCGCCAG CGAAGGGAGC GGCTCTAGGA AAAACTCCCT CAAGGACAAA 
ACCCGGTGGC AGTTTATAAT TGGAGATTTG CTGGATTCAG ACAATGACAT CTTTGAGAAG 
TCCAAAGAGT GCGACCCTCA TGGATCAGAG GAGTCACAGA AAGCCTTTGA CCATGGGACA 
GAGCTCATCC CATGGTATGT GCTGTCCATC CAAGCGGATG TGCACCAGTT CCTCCTGCAG 
GGGGCCACAG TCATCCACTA TGACCAGGAC ACACATCTCT CCGCCCGCTG CTTTCTCCAG 
TTGCAGCCTG ACAATAGTAC CTTGACCTGG ATGAAACCTC CCACTGCCTC CCCAGCTGGT 
GCCAGACTCA AGCTCGGTGT GCTTAGTAAC GTGGCCGAAC CTGGCAAGTT CCCATCACTG 
GGCAACGCTG GGGTGAGCGG CCTGGTAGAG GGAATCCTGG ATCTGTTTTC AGTGAAGGCT 
GTGTACATGG GACACCCTGG CATTGATATA CACACTGTGT GTGTCCAGAA CAAACTTAGT 
AGCATGCTTC TGTCAGAGAC TGGGGTGACG CTGCTCTATG GGCTTCAGAC CACCGACAAT 
AGATTACTGC ACTTCGTGGC ACCAAAGCAC ACAGCTAAAA TGCTTTTCAG TGGATTGCTG 
GAACTCACTA CAGCTGTGAG AAAGATACGG AAGTTCCCTG ACCAAAGACA ACAGTGGCTT 
CGGAAACAGT ATGTCAGCTT CTATCAGGAG GATGGTCGGT ATGAAGGCCC AACCTTGGCA 
CATGCTGTGG AGTTGTTTGG TGGTAGGAGA TGGAGCACTA GAAACCCCAG CCCTGGGATG 
TCAGCAAAGA ATGCCGAGAA GCCCAACATG CAGAGAAACA ACACCCTAGG CATAAGCACC 
ACCAAGAAAA AGAAGAAAAT GCTCATGAGG GGGGAGAGTG GAGAGGTGAC GGACGATGAG 
ATGGCCACCC GCAAGGCCAA AATGTACAGA GAGTGTCGCA GCCGGAGCGG CTCTGATCCT 
CAAGAGGCTA ATGAACAGGA AGACTCAGAG GCCAATGTCA TCACTAACCC TCCCAACCCC 
CTCCATTCCA GAAGAGCCTA CTCATTGACC ACGGCTGGGT CCCCCAACTT GGCTACCGGG 
ATGTCATCTC CTATCAGTGC CTGGAGTAGC AGCAGCTGGC ATGGGCGGAT CAAAGGAGGA 
ATGAAAGGCT TCCAGAGCTT CATGGTCTCA GACAGTAACA TGAGTTTCAT TGAATTCGTC 
GAGCTGTTCA AATCATTCAG CATAAGGAGC CGCAAGGACT TGAAGGACAT CTTTGACATC 
TACTCTGTAC CCTGTAATCG GTCTGCTTCT GAGTCAACCC CACTGTACAC CAACCTGACA 
ATCGAAGAAA ATACCAATGA CCTTCAGCCT GACCTAGATT TGCTGACTAG AAACGTCTCC 
GACTTGGGGC TGTTCATGAA GAGTAAGCAG CAGCTTTCTG ACAACCAGAG GCAGATATCT 
GACGCCATTG CTGCTGCAAG CATTGTGACT AATGGCACTG GGATTGAAAG CACGTCCCTG 
GGCATATTTG GGGTCGGCAT ACTCCAGCTC AACGACTTCC TGGTGAATTG CCAAGGAGAA 
CACTGCACAT ATGATGAAAT CCTCAGCATC ATCCAGAAGT TCGAGCCCAA CATCAGCATG 
TGTCATCAAG GTCTATTGTC CTTCGAAGGG TTTGCAAGGT TTCTGATGGA TAAAGACAAT 
TTCGCCTCAA AAAATGACGA GTCGCGGGAG AACAAGAAAG ACCTGCAGCT ACCCCTCTCC 
TACTATTACA TCGAATCTTC GCACAACACC TACCTCACCG GCCATCAGCT CAAGGGGGAG 
TCCTCTGTGG AGCTCTACAG CCAGGTCCTC TTGCAAGGAT GCAGAAGCAT AGAGCTGGAC 
TGCTGGGATG GAGATGATGG GATGCCCATT ATTTATCATG GACATACGCT GACAACCAAG 
ATCCCCTTCA AGGAAGTGGT GGAAGCCATC GATCGCAGTG CCTTCATCAC CTCCGACCTG 
CCAATCATCA TATCCATTGA GAACCATTGC TCATTGCCTC AGCAACGGAA AATGGCAGAA 
ATTTTCAAGA GTGTGTTTGG AGAAAAGCTG GTGGCTAAGT TCTTGTTCGA GACCGATTTC 
TCAGATGACC CAATGCTTCC CTCACCTGAC CAACTTAGAA GGAAAGTGCT TCTTAAAAAC 
AAGAAGCTAA AGGCCCATCA GACACCTGTC GACATCCTCA AACAAAAGGC TCATCAGTTA 
GCATCCATGC AAACACAGGC TTTCACTGGT GGGAATGCCA ACCCGCCACC TGCCAGTAAT 
GAGGAAGAGG AAGACGAGGA GGACGAATAT GATTATGATT ACGAGTCCCT GTCTGATGAC 
AACATTCTGG AAGACAGACC TGAGAACAAA TCATGTGCCG ACAAGCTTCA GTTTGAGTAC 
AATGAAGAAG TCCCCAAGAG GATAAAGAAA GCAGACAACT CCTCCGGCAA TAAGGGAAAG 
GTCTATGACA TGGAACTGGG AGAGGAGTTT TACCTTCCTC AGAACAAAAA AGAAAGCAGG 
CAGATTGCAC CGGAGCTCTC TGACCTCGTC ATCTATTGCC AGGCGGTAAA ATTTCCAGGC 
CTGTCAACTC TAAATTCATC TGGCTCTGGC AGAGGGAAAG AAAGGAAAAG CAGGAAGTCC 
ATTTTTGGCA ACAATCCCGG CAGAATGAGC CCAGGGGAGA CAGCGTCATT TAACAGAACA 
TCTGGAAAAA GTTCTTGTGA AGGAATTCGA CAAATCTGGG AGGAACCTCC ACTCAGCCCA 
AACACATCCC TCAGCGCCAT CATTAGAACT CCTAAATGCT ATCATATCTC GTCACTGAAT 
GAAAATGCCG CCAAACGTCT GTGTCGCAGG TATTCTCAGA AACTGATCCA GCACACTGCT 
TGCCAGCTGC TGAGGACCTA CCCGGCAGCG ACCAGAATCG ACTCATCCAA TCCCAACCCC 
CTCATGTTCT GGCTCCATGG GATACAGCTT GTAGCTCTAA ACTACCAGAC GGATGACCTT 
CCTTTACATT TAAACGCTGC CATGTTTGAG GCGAATGGTG GGTGTGGCTA TGTTTTGAAG 
CCCCCTGTCC TGTGGGATAA AAGCTGTCCC ATGTATCAGA AGTTTTCACC ACTGGAAAGA 
GACCTGGACG CCATGGATCC TGCTACCTAT TCTTTAACCA TCATCTCTGG CCAGAATGTG 
TGTCCTAGTA ACAGCACCGG AAGTCCGTGC ATTGAAGTTG ACGTCTTGGG CATGCCCCTG 
GACAGCTGCC ATTTCCGCAC AAAACCCATC CACCGAAACA CTCTGAACCC CATGTGGAAT 
GAGCAGTTTC TCTTCCGGGT TCACTTTGAA GATCTTGTAT TTCTTCGTTT TGCAGTTGTG 
GAGAACAACA GTTCGGCGAT AACGGCTCAG AGAATCATTC CACTCAAAGC TTTAAAGAGA 
GGATATCGGC ATCTTCAGCT GCGGAACCTC CACAACGAGA TCTTGGAAAT CTCTAGCTTA 
TTCATAAACA GCAGGAGGAT GGAAGATAAT CCCTCTGGCA GTACCCGGCC AGCCTCTTTG 
ATGTTCAATA CTGAAGAGAG GAAATGTTCT CAGACTCACA AAGTCACAGT GCATGGTGTC 
CCAGGTCCAG AGCCCTTTGC TGTTTTCACA ATAAACGAAG GCACCAAGGC AAAGCAGCTT 
CTCCAACAGA TTTTGGCAGT TGACCAGGAC ACCAAACTTA CAGCCGCAGA CTATTTTCTG 
ATGGAAGAGA AGCATTTTAT ATCTAAGGAA AAGAATGAAT GCAGGAAACA ACCATTCCAG 
AGAGCTGTGG GCCCAGAGGA AGATATTGTG CAGATTTTAA ACAGCTGGTT CCCAGAAGAA 
GGCTACGTAG GCAGGATTGT CTTAAAGCCC CAACAAGAAA CCCTAGAAGA GAAAAACATT 
GTCCACGATG ACAGAGAGGT GATCTTGAGC TCAGAAGAGG AGAGTTTCTT TGTCCAAGTG 
CACGATGTCT CTCCAGAGCA GCCTCGAACT GTCATCAAAG CCCCACGTGT CAGCACTGCA 
CAGGATGTGA TTCAGCAGAC CTTGTGCAAA GCCAAATATT CCTACAGCAT CCTGAACAAC 
CCCAATCCGT GTGACTATGT GCTTTTGGAA GAGGTAATGA AAGATGCACC CAATAAGAAG 
TCTTCTACCC CGAAGTCCTC ACAGAGGATC CTCTTGGACC AAGAATGTGT GTTTCAAGCC 
CAAAGCAAGT GGAAGGGTGC AGGGAAATTC ATCCTTAAGC TAAAGGAGCA GGTGCAGGCA 
TCCAGAGAAG ATAAAAGGAG GGGCATCTCC TTTGCAAGTG AACTCAAGAA ACTCACGAAG 
TCCACTAAGC AAACCCGAGG ACTCACCTCG CCTCCTCAGC TTGTGGCCTC AGAAAGTGTG 
CAAAGCAAGG AGGAGAAACC CATGGGTGCC TTGGCCTCTG GTGACACAGC GGGCTACCAG 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446210.1
CDS	564..7409
Translation	MTSEEMAASFLIPVPQRKVASAQSVAEERGEKVSEAGIPKTRAGRQGGLTPRTISQRNEPEEESPRTDFSQVFSIARGELDSDENHNERCWEENVPGSTKNHAVNCNSLLQSHQHALPPSQLCEVCDSVTEEHLCLQPGIPSPLERKVFPGIELEMEDSPMDVSPLGNQPGIMESSGPHSDRNMAVFHFHYAGDRTMPGAFHTLSEKFILDDCANCVTLPGGQQNKNYMAYTCKLVELTRTCGSKNGQLKCDHCTSLRDEYLCFESSCRKAEALSSGGGFCEDGFTHGPSAKTFLNPLEEFSDNCEDVDDIFKGKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLAAWSYVDQKKAGLMWPCGNGMRPLSTVDVRQSGRQRLSEAQWCLIYSAVRREETEDTVGSLLHCSTQLPTPDTAHGRIGDGPCLKQCVRDSECEYRATLQRTSIAQYITGSLLEATTSLGARSSLLSSFGGSTGRIMLKERQPGTSMANSSPVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRRVLPMDYLCFLTRDLSSPECQRSLPRLKACISESILMSQSGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSVEYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEEALEFVADYSGQDNFLQRVGQNGLKNPEKELTVNSIFQIIRSCSRSLETEDEESASEGSGSRKNSLKDKTRWQFIIGDLLDSDNDIFEKSKECDPHGSEESQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTASPAGARLKLGVLSNVAEPGKFPSLGNAGVSGLVEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSMLLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTTAVRKIRKFPDQRQQWLRKQYVSFYQEDGRYEGPTLAHAVELFGGRRWSTRNPSPGMSAKNAEKPNMQRNNTLGISTTKKKKKMLMRGESGEVTDDEMATRKAKMYRECRSRSGSDPQEANEQEDSEANVITNPPNPLHSRRAYSLTTAGSPNLATGMSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFIEFVELFKSFSIRSRKDLKDIFDIYSVPCNRSASESTPLYTNLTIEENTNDLQPDLDLLTRNVSDLGLFMKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPNISMCHQGLLSFEGFARFLMDKDNFASKNDESRENKKDLQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQLASMQTQAFTGGNANPPPASNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCADKLQFEYNEEVPKRIKKADNSSGNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNSSGSGRGKERKSRKSIFGNNPGRMSPGETASFNRTSGKSSCEGIRQIWEEPPLSPNTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERDLDAMDPATYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLKALKRGYRHLQLRNLHNEILEISSLFINSRRMEDNPSGSTRPASLMFNTEERKCSQTHKVTVHGVPGPEPFAVFTINEGTKAKQLLQQILAVDQDTKLTAADYFLMEEKHFISKEKNECRKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKNIVHDDREVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPCDYVLLEEVMKDAPNKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRRGISFASELKKLTKSTKQTRGLTSPPQLVASESVQSKEEKPMGALASGDTAGYQS

Target ORF information:

RefSeq Version	NM_053758.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C, epsilon 1 (Plce1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053758.2

ORF Insert Sequence:

ATGACGTCCG AAGAAATGGC CGCTTCCTTC CTCATCCCGG TGCCTCAAAG GAAAGTGGCT 
TCTGCCCAGT CAGTGGCAGA GGAGCGGGGT GAAAAGGTCT CAGAAGCCGG CATTCCTAAG 
ACACGAGCTG GCAGACAGGG TGGGCTCACC CCTCGTACCA TCTCACAGCG GAACGAACCT 
GAGGAAGAGT CTCCTAGAAC TGATTTTTCC CAGGTCTTCT CAATAGCGAG GGGGGAACTA 
GACAGCGACG AGAATCACAA TGAAAGGTGC TGGGAGGAAA ATGTGCCAGG TTCCACGAAA 
AACCACGCGG TTAACTGCAA CAGCTTATTG CAAAGCCACC AGCATGCGCT TCCCCCGAGC 
CAGCTCTGTG AAGTCTGTGA CTCTGTCACA GAAGAACACC TGTGTTTGCA GCCTGGGATT 
CCTTCTCCAC TGGAAAGGAA GGTGTTTCCT GGGATCGAAC TGGAGATGGA GGACTCTCCC 
ATGGACGTGA GCCCTTTGGG AAACCAGCCT GGGATCATGG AGTCCAGCGG ACCTCACTCT 
GACCGCAACA TGGCGGTATT TCATTTCCAT TATGCAGGTG ACAGGACAAT GCCAGGTGCT 
TTCCATACCC TGTCAGAAAA ATTCATTTTG GATGACTGTG CAAACTGTGT TACTCTTCCT 
GGGGGGCAGC AAAACAAAAA TTACATGGCG TATACTTGCA AACTGGTGGA GTTGACAAGA 
ACCTGTGGTA GTAAGAATGG GCAGCTGAAG TGTGACCACT GCACCTCGCT GCGGGATGAA 
TACCTGTGCT TTGAAAGCTC CTGCCGGAAG GCTGAGGCTC TCTCCTCAGG TGGTGGCTTT 
TGCGAGGACG GATTTACTCA TGGTCCCTCT GCCAAGACTT TTCTGAACCC TTTGGAGGAA 
TTCTCTGATA ATTGTGAAGA CGTGGACGAT ATTTTTAAAG GCAAAAAGGA GCGGTCCACT 
TTGTTAGTCC GAAGGTTTTG TAAAAATGAC AGGGAAGTTA AGAAGTCTGT GTATACTGGG 
ACAAGGGCCA TTGTGAGAAC CCTGCCTTCT GGCCACATTG GGCTGGCTGC TTGGAGTTAC 
GTCGATCAGA AGAAAGCTGG TCTCATGTGG CCTTGTGGGA ATGGAATGAG ACCTCTGTCC 
ACAGTGGACG TAAGGCAAAG TGGGAGACAG CGTCTGTCTG AAGCCCAGTG GTGTCTGATC 
TACAGTGCCG TGAGAAGAGA AGAGACAGAA GATACGGTTG GCTCTCTTCT GCATTGCTCC 
ACACAGCTCC CAACCCCTGA CACAGCCCAT GGAAGGATCG GAGATGGTCC CTGCCTAAAG 
CAATGTGTGC GAGACAGCGA GTGTGAGTAC CGTGCCACCC TCCAAAGGAC GTCCATAGCT 
CAGTACATCA CCGGTTCTCT CCTAGAGGCA ACCACATCTT TAGGAGCAAG AAGCAGCCTT 
CTCAGTTCCT TTGGAGGATC CACTGGGCGA ATAATGCTGA AAGAACGCCA ACCGGGCACT 
TCTATGGCCA ATTCCAGTCC TGTTCCTTCA AGTTCAGCTG GAATCAGCAA GGAGCTGATT 
GATCTCCAGC CCCTCATTCA GTTCCCGGAG GAAGTAGCCA GCATCTTAAC AGAACAGGAA 
CAGAACATTT ACCGCAGGGT CCTGCCCATG GACTACCTCT GTTTCTTAAC CCGGGACTTG 
AGTTCCCCCG AGTGTCAGAG ATCACTGCCC CGTCTCAAAG CATGCATCTC AGAATCAATC 
CTCATGTCTC AAAGTGGAGA GCACAATGCC CTTGAAGACC TCGTGATGAG ATTTAACGAG 
GTGAGCTCCT GGGTAACATG GCTGATCCTC ACGGCAGGCT CCATGGAGGA GAAGCGGGAA 
GTCTTTTCTT ATTTGGTGCA TGTGGCCAAA TGCTGCTGGA ACATGGGCAA CTACAATGCT 
GTCATGGAGT TCCTGGCTGG CCTCAGGTCA AGGAAAGTTC TGAAGATGTG GCAGTTCATG 
GACCAGTCTG ACATCGAGAC CATGAGGAGC CTGAAAGACG CCATGGCTCA GCATGAGTCT 
TCCGTGGAGT ACAAGAAGGT AGTGACCCGA GCCTTGCACA TCCCCGGCTG CAAAGTGGTT 
CCCTTCTGTG GGGTGTTCCT GAAGGAGCTC TGTGAGGTGC TTGATGGTGC CTCTGGCCTC 
CTGAAGCTCT GCCCACGATA CAGCTCCCAA GAAGAAGCCC TGGAGTTTGT AGCGGATTAC 
AGTGGACAAG ATAACTTCTT ACAGCGAGTG GGGCAGAATG GCTTGAAGAA TCCAGAGAAG 
GAGCTCACGG TCAACAGCAT CTTTCAGATC ATCCGGAGCT GCAGCCGAAG CTTGGAGACG 
GAGGACGAGG AGAGCGCCAG CGAAGGGAGC GGCTCTAGGA AAAACTCCCT CAAGGACAAA 
ACCCGGTGGC AGTTTATAAT TGGAGATTTG CTGGATTCAG ACAATGACAT CTTTGAGAAG 
TCCAAAGAGT GCGACCCTCA TGGATCAGAG GAGTCACAGA AAGCCTTTGA CCATGGGACA 
GAGCTCATCC CATGGTATGT GCTGTCCATC CAAGCGGATG TGCACCAGTT CCTCCTGCAG 
GGGGCCACAG TCATCCACTA TGACCAGGAC ACACATCTCT CCGCCCGCTG CTTTCTCCAG 
TTGCAGCCTG ACAATAGTAC CTTGACCTGG ATGAAACCTC CCACTGCCTC CCCAGCTGGT 
GCCAGACTCA AGCTCGGTGT GCTTAGTAAC GTGGCCGAAC CTGGCAAGTT CCCATCACTG 
GGCAACGCTG GGGTGAGCGG CCTGGTAGAG GGAATCCTGG ATCTGTTTTC AGTGAAGGCT 
GTGTACATGG GACACCCTGG CATTGATATA CACACTGTGT GTGTCCAGAA CAAACTTAGT 
AGCATGCTTC TGTCAGAGAC TGGGGTGACG CTGCTCTATG GGCTTCAGAC CACCGACAAT 
AGATTACTGC ACTTCGTGGC ACCAAAGCAC ACAGCTAAAA TGCTTTTCAG TGGATTGCTG 
GAACTCACTA CAGCTGTGAG AAAGATACGG AAGTTCCCTG ACCAAAGACA ACAGTGGCTT 
CGGAAACAGT ATGTCAGCTT CTATCAGGAG GATGGTCGGT ATGAAGGCCC AACCTTGGCA 
CATGCTGTGG AGTTGTTTGG TGGTAGGAGA TGGAGCACTA GAAACCCCAG CCCTGGGATG 
TCAGCAAAGA ATGCCGAGAA GCCCAACATG CAGAGAAACA ACACCCTAGG CATAAGCACC 
ACCAAGAAAA AGAAGAAAAT GCTCATGAGG GGGGAGAGTG GAGAGGTGAC GGACGATGAG 
ATGGCCACCC GCAAGGCCAA AATGTACAGA GAGTGTCGCA GCCGGAGCGG CTCTGATCCT 
CAAGAGGCTA ATGAACAGGA AGACTCAGAG GCCAATGTCA TCACTAACCC TCCCAACCCC 
CTCCATTCCA GAAGAGCCTA CTCATTGACC ACGGCTGGGT CCCCCAACTT GGCTACCGGG 
ATGTCATCTC CTATCAGTGC CTGGAGTAGC AGCAGCTGGC ATGGGCGGAT CAAAGGAGGA 
ATGAAAGGCT TCCAGAGCTT CATGGTCTCA GACAGTAACA TGAGTTTCAT TGAATTCGTC 
GAGCTGTTCA AATCATTCAG CATAAGGAGC CGCAAGGACT TGAAGGACAT CTTTGACATC 
TACTCTGTAC CCTGTAATCG GTCTGCTTCT GAGTCAACCC CACTGTACAC CAACCTGACA 
ATCGAAGAAA ATACCAATGA CCTTCAGCCT GACCTAGATT TGCTGACTAG AAACGTCTCC 
GACTTGGGGC TGTTCATGAA GAGTAAGCAG CAGCTTTCTG ACAACCAGAG GCAGATATCT 
GACGCCATTG CTGCTGCAAG CATTGTGACT AATGGCACTG GGATTGAAAG CACGTCCCTG 
GGCATATTTG GGGTCGGCAT ACTCCAGCTC AACGACTTCC TGGTGAATTG CCAAGGAGAA 
CACTGCACAT ATGATGAAAT CCTCAGCATC ATCCAGAAGT TCGAGCCCAA CATCAGCATG 
TGTCATCAAG GTCTATTGTC CTTCGAAGGG TTTGCAAGGT TTCTGATGGA TAAAGACAAT 
TTCGCCTCAA AAAATGACGA GTCGCGGGAG AACAAGAAAG ACCTGCAGCT ACCCCTCTCC 
TACTATTACA TCGAATCTTC GCACAACACC TACCTCACCG GCCATCAGCT CAAGGGGGAG 
TCCTCTGTGG AGCTCTACAG CCAGGTCCTC TTGCAAGGAT GCAGAAGCAT AGAGCTGGAC 
TGCTGGGATG GAGATGATGG GATGCCCATT ATTTATCATG GACATACGCT GACAACCAAG 
ATCCCCTTCA AGGAAGTGGT GGAAGCCATC GATCGCAGTG CCTTCATCAC CTCCGACCTG 
CCAATCATCA TATCCATTGA GAACCATTGC TCATTGCCTC AGCAACGGAA AATGGCAGAA 
ATTTTCAAGA GTGTGTTTGG AGAAAAGCTG GTGGCTAAGT TCTTGTTCGA GACCGATTTC 
TCAGATGACC CAATGCTTCC CTCACCTGAC CAACTTAGAA GGAAAGTGCT TCTTAAAAAC 
AAGAAGCTAA AGGCCCATCA GACACCTGTC GACATCCTCA AACAAAAGGC TCATCAGTTA 
GCATCCATGC AAACACAGGC TTTCACTGGT GGGAATGCCA ACCCGCCACC TGCCAGTAAT 
GAGGAAGAGG AAGACGAGGA GGACGAATAT GATTATGATT ACGAGTCCCT GTCTGATGAC 
AACATTCTGG AAGACAGACC TGAGAACAAA TCATGTGCCG ACAAGCTTCA GTTTGAGTAC 
AATGAAGAAG TCCCCAAGAG GATAAAGAAA GCAGACAACT CCTCCGGCAA TAAGGGAAAG 
GTCTATGACA TGGAACTGGG AGAGGAGTTT TACCTTCCTC AGAACAAAAA AGAAAGCAGG 
CAGATTGCAC CGGAGCTCTC TGACCTCGTC ATCTATTGCC AGGCGGTAAA ATTTCCAGGC 
CTGTCAACTC TAAATTCATC TGGCTCTGGC AGAGGGAAAG AAAGGAAAAG CAGGAAGTCC 
ATTTTTGGCA ACAATCCCGG CAGAATGAGC CCAGGGGAGA CAGCGTCATT TAACAGAACA 
TCTGGAAAAA GTTCTTGTGA AGGAATTCGA CAAATCTGGG AGGAACCTCC ACTCAGCCCA 
AACACATCCC TCAGCGCCAT CATTAGAACT CCTAAATGCT ATCATATCTC GTCACTGAAT 
GAAAATGCCG CCAAACGTCT GTGTCGCAGG TATTCTCAGA AACTGATCCA GCACACTGCT 
TGCCAGCTGC TGAGGACCTA CCCGGCAGCG ACCAGAATCG ACTCATCCAA TCCCAACCCC 
CTCATGTTCT GGCTCCATGG GATACAGCTT GTAGCTCTAA ACTACCAGAC GGATGACCTT 
CCTTTACATT TAAACGCTGC CATGTTTGAG GCGAATGGTG GGTGTGGCTA TGTTTTGAAG 
CCCCCTGTCC TGTGGGATAA AAGCTGTCCC ATGTATCAGA AGTTTTCACC ACTGGAAAGA 
GACCTGGACG CCATGGATCC TGCTACCTAT TCTTTAACCA TCATCTCTGG CCAGAATGTG 
TGTCCTAGTA ACAGCACCGG AAGTCCGTGC ATTGAAGTTG ACGTCTTGGG CATGCCCCTG 
GACAGCTGCC ATTTCCGCAC AAAACCCATC CACCGAAACA CTCTGAACCC CATGTGGAAT 
GAGCAGTTTC TCTTCCGGGT TCACTTTGAA GATCTTGTAT TTCTTCGTTT TGCAGTTGTG 
GAGAACAACA GTTCGGCGAT AACGGCTCAG AGAATCATTC CACTCAAAGC TTTAAAGAGA 
GGATATCGGC ATCTTCAGCT GCGGAACCTC CACAACGAGA TCTTGGAAAT CTCTAGCTTA 
TTCATAAACA GCAGGAGGAT GGAAGATAAT CCCTCTGGCA GTACCCGGCC AGCCTCTTTG 
ATGTTCAATA CTGAAGAGAG GAAATGTTCT CAGACTCACA AAGTCACAGT GCATGGTGTC 
CCAGGTCCAG AGCCCTTTGC TGTTTTCACA ATAAACGAAG GCACCAAGGC AAAGCAGCTT 
CTCCAACAGA TTTTGGCAGT TGACCAGGAC ACCAAACTTA CAGCCGCAGA CTATTTTCTG 
ATGGAAGAGA AGCATTTTAT ATCTAAGGAA AAGAATGAAT GCAGGAAACA ACCATTCCAG 
AGAGCTGTGG GCCCAGAGGA AGATATTGTG CAGATTTTAA ACAGCTGGTT CCCAGAAGAA 
GGCTACGTAG GCAGGATTGT CTTAAAGCCC CAACAAGAAA CCCTAGAAGA GAAAAACATT 
GTCCACGATG ACAGAGAGGT GATCTTGAGC TCAGAAGAGG AGAGTTTCTT TGTCCAAGTG 
CACGATGTCT CTCCAGAGCA GCCTCGAACT GTCATCAAAG CCCCACGTGT CAGCACTGCA 
CAGGATGTGA TTCAGCAGAC CTTGTGCAAA GCCAAATATT CCTACAGCAT CCTGAACAAC 
CCCAATCCGT GTGACTATGT GCTTTTGGAA GAGGTAATGA AAGATGCACC CAATAAGAAG 
TCTTCTACCC CGAAGTCCTC ACAGAGGATC CTCTTGGACC AAGAATGTGT GTTTCAAGCC 
CAAAGCAAGT GGAAGGGTGC AGGGAAATTC ATCCTTAAGC TAAAGGAGCA GGTGCAGGCA 
TCCAGAGAAG ATAAAAGGAG GGGCATCTCC TTTGCAAGTG AACTCAAGAA ACTCACGAAG 
TCCACTAAGC AAACCCGAGG ACTCACCTCG CCTCCTCAGC TTGTGGCCTC AGAAAGTGTG 
CAAAGCAAGG AGGAGAAACC CATGGGTGCC TTGGCCTCTG GTGACACAGC GGGCTACCAG 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plce1 ( XM_017588656.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444145.1 Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X1, mRNA.

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CloneID	ORa56295
Clone ID Related Accession (Same CDS sequence)	XM_017588656.1 , XM_017588657.1 , XM_017588658.1
Accession Version	XM_017588656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCCG AAGAAATGGC CGCTTCCTTC CTCATCCCGG TGCCTCAAAG GAAAGTGGCT 
TCTGCCCAGT CAGTGGCAGA GGAGCGGGGT GAAAAGGTCT CAGAAGCCGG CATTCCTAAG 
ACACGAGCTG GCAGACAGGG TGGGCTCACC CCTCGTACCA TCTCACAGCG GAACGAACCT 
GAGGAAGAGT CTCCTAGAAC TGATTTTTCC CAGGTCTTCT CAATAGCGAG GGGGGAACTA 
GACAGCGACG AGAATCACAA TGAAAGGTGC TGGGAGGAAA ATGTGCCAGG TTCCACGAAA 
AACCACGCGG TTAACTGCAA CAGCTTATTG CAAAGCCACC AGCATGCGCT TCCCCCGAGC 
CAGCTCTGTG AAGTCTGTGA CTCTGTCACA GAAGAACACC TGTGTTTGCA GCCTGGGATT 
CCTTCTCCAC TGGAAAGGAA GGTGTTTCCT GGGATCGAAC TGGAGATGGA GGACTCTCCC 
ATGGACGTGA GCCCTTTGGG AAACCAGCCT GGGATCATGG AGTCCAGCGG ACCTCACTCT 
GACCGCAACA TGGCGGTATT TCATTTCCAT TATGCAGGTG ACAGGACAAT GCCAGGTGCT 
TTCCATACCC TGTCAGAAAA ATTCATTTTG GATGACTGTG CAAACTGTGT TACTCTTCCT 
GGGGGGCAGC AAAACAAAAA TTACATGGCG TATACTTGCA AACTGGTGGA GTTGACAAGA 
ACCTGTGGTA GTAAGAATGG GCAGCTGAAG TGTGACCACT GCACCTCGCT GCGGGATGAA 
TACCTGTGCT TTGAAAGCTC CTGCCGGAAG GCTGAGGCTC TCTCCTCAGG TGGTGGCTTT 
TGCGAGGACG GATTTACTCA TGGTCCCTCT GCCAAGACTT TTCTGAACCC TTTGGAGGAA 
TTCTCTGATA ATTGTGAAGA CGTGGACGAT ATTTTTAAAG GCAAAAAGGA GCGGTCCACT 
TTGTTAGTCC GAAGGTTTTG TAAAAATGAC AGGGAAGTTA AGAAGTCTGT GTATACTGGG 
ACAAGGGCCA TTGTGAGAAC CCTGCCTTCT GGCCACATTG GGCTGGCTGC TTGGAGTTAC 
GTCGATCAGA AGAAAGCTGG TCTCATGTGG CCTTGTGGGA ATGGAATGAG ACCTCTGTCC 
ACAGTGGACG TAAGGCAAAG TGGGAGACAG CGTCTGTCTG AAGCCCAGTG GTGTCTGATC 
TACAGTGCCG TGAGAAGAGA AGAGACAGAA GATACGGTTG GCTCTCTTCT GCATTGCTCC 
ACACAGCTCC CAACCCCTGA CACAGCCCAT GGAAGGATCG GAGATGGTCC CTGCCTAAAG 
CAATGTGTGC GAGACAGCGA GTGTGAGTAC CGTGCCACCC TCCAAAGGAC GTCCATAGCT 
CAGTACATCA CCGGTTCTCT CCTAGAGGCA ACCACATCTT TAGGAGCAAG AAGCAGCCTT 
CTCAGTTCCT TTGGAGGATC CACTGGGCGA ATAATGCTGA AAGAACGCCA ACCGGGCACT 
TCTATGGCCA ATTCCAGTCC TGTTCCTTCA AGTTCAGCTG GAATCAGCAA GGAGCTGATT 
GATCTCCAGC CCCTCATTCA GTTCCCGGAG GAAGTAGCCA GCATCTTAAC AGAACAGGAA 
CAGAACATTT ACCGCAGGGT CCTGCCCATG GACTACCTCT GTTTCTTAAC CCGGGACTTG 
AGTTCCCCCG AGTGTCAGAG ATCACTGCCC CGTCTCAAAG CATGCATCTC AGAATCAATC 
CTCATGTCTC AAAGTGGAGA GCACAATGCC CTTGAAGACC TCGTGATGAG ATTTAACGAG 
GTGAGCTCCT GGGTAACATG GCTGATCCTC ACGGCAGGCT CCATGGAGGA GAAGCGGGAA 
GTCTTTTCTT ATTTGGTGCA TGTGGCCAAA TGCTGCTGGA ACATGGGCAA CTACAATGCT 
GTCATGGAGT TCCTGGCTGG CCTCAGGTCA AGGAAAGTTC TGAAGATGTG GCAGTTCATG 
GACCAGTCTG ACATCGAGAC CATGAGGAGC CTGAAAGACG CCATGGCTCA GCATGAGTCT 
TCCGTGGAGT ACAAGAAGGT AGTGACCCGA GCCTTGCACA TCCCCGGCTG CAAAGTGGTT 
CCTTTCTGTG GGGTGTTCCT GAAGGAGCTC TGTGAGGTGC TTGATGGTGC CTCTGGCCTC 
CTGAAGCTCT GCCCACGATA CAGCTCCCAA GAAGAAGCCC TGGAGTTTGT AGCGGATTAC 
AGTGGACAAG ATAACTTCTT ACAGCGAGTG GGGCAGAATG GCTTGAAGAA TCCAGAGAAG 
GAGCTCACGG TCAACAGCAT CTTTCAGATC ATCCGGAGCT GCAGCCGAAG CTTGGAGACG 
GAGGACGAGG AGAGCGCCAG CGAAGGGAGC GGCTCTAGGA AAAACTCCCT CAAGGACAAA 
ACCCGGTGGC AGTTTATAAT TGGAGATTTG CTGGATTCAG ACAATGACAT CTTTGAGAAG 
TCCAAAGAGT GCGACCCTCA TGGATCAGAG GAGTCACAGA AAGCCTTTGA CCATGGGACA 
GAGCTCATCC CATGGTATGT GCTGTCCATC CAAGCGGATG TGCACCAGTT CCTCCTGCAG 
GGGGCCACAG TCATCCACTA TGACCAGGAC ACACATCTCT CCGCCCGCTG CTTTCTCCAG 
TTGCAGCCTG ACAATAGTAC CTTGACCTGG ATGAAACCTC CCACTGCCTC CCCAGCTGGT 
GCCAGACTCA AGCTCGGTGT GCTTAGTAAC GTGGCCGAAC CTGGCAAGTT CCCATCACTG 
GGCAACGCTG GGGTGAGCGG CCTGGTAGAG GGAATCCTGG ATCTGTTTTC AGTGAAGGCT 
GTGTACATGG GACACCCTGG CATTGATATA CACACTGTGT GTGTCCAGAA CAAACTTAGT 
AGCATGCTTC TGTCAGAGAC TGGGGTGACG CTGCTCTATG GGCTTCAGAC CACCGACAAT 
AGATTACTGC ACTTCGTGGC ACCAAAGCAC ACAGCTAAAA TGCTTTTCAG TGGATTGCTG 
GAACTCACTA CAGCTGTGAG AAAGATACGG AAGTTCCCTG ACCAAAGACA ACAGTGGCTT 
CGGAAACAGT ATGTCAGCTT CTATCAGGAG GATGGTCGGT ATGAAGGCCC AACCTTGGCA 
CATGCTGTGG AGTTGTTTGG TGGTAGGAGA TGGAGCACTA GAAACCCCAG CCCTGGGATG 
TCAGCAAAGA ATGCCGAGAA GCCCAACATG CAGAGAAACA ACACCCTAGG CATAAGCACC 
ACCAAGAAAA AGAAGAAAAT GCTCATGAGG GGGGAGAGTG GAGAGGTGAC GGACGATGAG 
ATGGCCACCC GCAAGGCCAA AATGTACAGA GAGTGTCGCA GCCGGAGCGG CTCTGATCCT 
CAAGAGGCTA ATGAACAGGA AGACTCAGAG GCCAATGTCA TCACTAACCC TCCCAACCCC 
CTCCATTCCA GAAGAGCCTA CTCATTGACC ACGGCTGGGT CCCCCAACTT GGCTACCGGG 
ATGTCATCTC CTATCAGTGC CTGGAGTAGC AGCAGCTGGC ACGGGCGGAT CAAAGGAGGA 
ATGAAAGGCT TCCAGAGCTT CATGGTCTCA GACAGTAACA TGAGTTTCAT TGAATTTGTC 
GAGCTGTTCA AATCATTCAG CATAAGGAGC CGCAAGGACT TGAAGGACAT CTTTGACATC 
TACTCTGTAC CCTGCAATCG GTCTGCTTCT GAGTCAGCCC CACTGTACAC CAACCTGACA 
ATCGAAGAAA ATACCAATGA CCTTCAGCCT GACCTAGATT TGCTGACTAG AAACGTCTCC 
GACTTGGGGC TGTTCATGAA GAGTAAGCAG CAGCTTTCTG ACAACCAGAG GCAGATATCT 
GACGCCATTG CTGCTGCAAG CATTGTGACT AATGGCACTG GGATTGAAAG CACGTCCCTG 
GGCATATTTG GGGTCGGCAT ACTCCAGCTC AACGACTTCC TGGTGAATTG CCAAGGAGAA 
CACTGCACAT ATGATGAAAT CCTCAGCATC ATCCAGAAGT TCGAGCCCAA CATCAGCATG 
TGTCATCAAG GTCTATTGTC CTTCGAAGGG TTTGCAAGGT TTCTGATGGA TAAAGACAAT 
TTCGCCTCAA AAAATGACGA GTCGCGGGAG AACAAGAAAG ACCTGCAGCT ACCCCTCTCC 
TACTATTACA TCGAATCTTC GCACAACACC TACCTCACCG GCCATCAGCT CAAGGGGGAG 
TCCTCTGTGG AGCTCTACAG CCAGGTCCTC TTGCAAGGAT GCAGAAGCAT AGAGCTGGAC 
TGCTGGGATG GAGATGATGG GATGCCCATT ATTTATCATG GACATACGCT GACAACCAAG 
ATCCCCTTCA AGGAAGTGGT GGAAGCCATC GATCGCAGTG CCTTCATCAC CTCCGACCTG 
CCAATCATCA TATCCATTGA GAACCATTGC TCATTGCCTC AGCAACGGAA AATGGCAGAA 
ATTTTCAAGA GTGTGTTTGG AGAAAAGCTG GTGGCTAAGT TCTTGTTCGA GACCGATTTC 
TCAGATGACC CAATGCTTCC CTCACCTGAC CAACTTAGAA GGAAAGTGCT TCTTAAAAAC 
AAGAAGCTAA AGGCCCATCA GACACCTGTC GACATCCTCA AACAAAAGGC TCATCAGTTA 
GCATCCATGC AAACACAGGC TTTCACTGGT GGGAATGCCA ACCCGCCACC TGCCAGTAAT 
GAGGAAGAGG AAGACGAGGA GGACGAATAT GATTATGATT ACGAGTCCCT GTCTGATGCA 
GATGTCCTTA CTGCTTCCCC TGTTCCTAAC AGTCAGGAAG ACAACATTCT GGAAGACAGA 
CCTGAGAACA AATCATGTGC CGACAAGCTT CAGTTTGAGT ACAATGAAGA AGTCCCCAAG 
AGGATAAAGA AAGCAGACAA CTCCTCCGGC AATAAGGGAA AGGTCTATGA CATGGAACTG 
GGAGAGGAGT TTTACCTTCC TCAGAACAAA AAAGAAAGCA GGCAGATTGC ACCGGAGCTC 
TCTGACCTCG TCATCTATTG CCAGGCGGTA AAATTTCCAG GCCTGTCAAC TCTAAATTCA 
TCTGGCTCTG GCAGAGGGAA AGAAAGGAAA AGCAGGAAGT CCATTTTTGG CAACAATCCC 
GGCAGAATGA GCCCAGGGGA GACAGCGTCA TTTAACAGAA CATCTGGAAA AAGTTCTTGT 
GAAGGAATTC GACAAATCTG GGAGGAACCT CCACTCAGCC CAAACACATC CCTCAGCGCC 
ATCATTAGAA CTCCTAAATG CTATCATATC TCGTCACTGA ATGAAAATGC CGCCAAACGT 
CTGTGTCGCA GGTATTCTCA GAAACTGATC CAGCACACTG CTTGCCAGCT GCTGAGGACC 
TACCCGGCAG CGACCAGAAT CGACTCATCC AATCCCAACC CCCTCATGTT CTGGCTCCAT 
GGGATACAGC TTGTAGCTCT AAACTACCAG ACGGATGACC TTCCTTTACA TTTAAACGCT 
GCCATGTTTG AGGCGAATGG TGGGTGTGGC TATGTTTTGA AGCCCCCTGT CCTGTGGGAT 
AAAAGCTGTC CCATGTATCA GAAGTTTTCA CCACTGGAAA GAGACCTGGA CGCCATGGAT 
CCTGCTACCT ATTCTTTAAC CATCATCTCT GGCCAGAATG TGTGTCCTAG TAACAGCACC 
GGAAGTCCGT GCATTGAAGT TGACGTCTTG GGCATGCCCC TGGACAGCTG CCATTTCCGC 
ACAAAACCCA TCCACCGAAA CACTCTGAAC CCCATGTGGA ATGAGCAGTT TCTCTTCCGG 
GTTCACTTTG AAGATCTTGT ATTTCTTCGT TTTGCAGTTG TGGAGAACAA CAGTTCGGCG 
ATAACGGCTC AGAGAATCAT TCCACTCAAA GCTTTAAAGA GAGGATATCG GCATCTTCAG 
CTGCGGAACC TCCACAACGA GATCTTGGAA ATCTCTAGCT TATTCATAAA CAGCAGGAGG 
ATGGAAGATA ATCCCTCTGG CAGTACCCGG CCAGCCTCTT TGATGTTCAA TACTGAAGAG 
AGGAAATGTT CTCAGACTCA CAAAGTCACA GTGCATGGTG TCCCAGGTCC AGAGCCCTTT 
GCTGTTTTCA CAATAAACGA AGGCACCAAG GCAAAGCAGC TTCTCCAACA GATTTTGGCA 
GTTGACCAGG ACACCAAACT TACAGCCGCA GACTATTTTC TGATGGAAGA GAAGCATTTT 
ATATCTAAGG AAAAGAATGA ATGCAGGAAA CAACCATTCC AGAGAGCTGT GGGCCCAGAG 
GAAGATATTG TGCAGATTTT AAACAGCTGG TTCCCAGAAG AAGGCTACGT AGGCAGGATT 
GTCTTAAAGC CCCAACAAGA AACCCTAGAA GAGAAAAACA TTGTCCACGA TGACAGAGAG 
GTGATCTTGA GCTCAGAAGA GGAGAGTTTC TTTGTCCAAG TGCACGATGT CTCTCCAGAG 
CAGCCTCGAA CTGTCATCAA AGCCCCACGT GTCAGCACTG CACAGGATGT GATTCAGCAG 
ACCTTGTGCA AAGCCAAATA TTCCTACAGC ATCCTGAACA ACCCCAATCC GTGTGACTAT 
GTGCTTTTGG AAGAGGTAAT GAAAGATGCA CCCAATAAGA AGTCTTCTAC CCCGAAGTCC 
TCACAGAGGA TCCTCTTGGA CCAAGAATGT GTGTTTCAAG CCCAAAGCAA GTGGAAGGGT 
GCAGGGAAAT TCATCCTTAA GCTAAAGGAG CAGGTGCAGG CATCCAGAGA AGATAAAAGG 
AGGGGCATCT CCTTTGCAAG TGAACTCAAG AAACTCACGA AGTCCACTAA GCAAACCCGA 
GGACTCACCT CGCCTCCTCA GCTTGTGGCC TCAGAAAGTG TGCAAAGCAA GGAGGAGAAA 
CCCATGGGTG CCTTGGCCTC TGGTGACACA GCGGGCTACC AGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444145.1
CDS	689..7576
Translation	MTSEEMAASFLIPVPQRKVASAQSVAEERGEKVSEAGIPKTRAGRQGGLTPRTISQRNEPEEESPRTDFSQVFSIARGELDSDENHNERCWEENVPGSTKNHAVNCNSLLQSHQHALPPSQLCEVCDSVTEEHLCLQPGIPSPLERKVFPGIELEMEDSPMDVSPLGNQPGIMESSGPHSDRNMAVFHFHYAGDRTMPGAFHTLSEKFILDDCANCVTLPGGQQNKNYMAYTCKLVELTRTCGSKNGQLKCDHCTSLRDEYLCFESSCRKAEALSSGGGFCEDGFTHGPSAKTFLNPLEEFSDNCEDVDDIFKGKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLAAWSYVDQKKAGLMWPCGNGMRPLSTVDVRQSGRQRLSEAQWCLIYSAVRREETEDTVGSLLHCSTQLPTPDTAHGRIGDGPCLKQCVRDSECEYRATLQRTSIAQYITGSLLEATTSLGARSSLLSSFGGSTGRIMLKERQPGTSMANSSPVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRRVLPMDYLCFLTRDLSSPECQRSLPRLKACISESILMSQSGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSVEYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEEALEFVADYSGQDNFLQRVGQNGLKNPEKELTVNSIFQIIRSCSRSLETEDEESASEGSGSRKNSLKDKTRWQFIIGDLLDSDNDIFEKSKECDPHGSEESQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTASPAGARLKLGVLSNVAEPGKFPSLGNAGVSGLVEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSMLLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTTAVRKIRKFPDQRQQWLRKQYVSFYQEDGRYEGPTLAHAVELFGGRRWSTRNPSPGMSAKNAEKPNMQRNNTLGISTTKKKKKMLMRGESGEVTDDEMATRKAKMYRECRSRSGSDPQEANEQEDSEANVITNPPNPLHSRRAYSLTTAGSPNLATGMSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFIEFVELFKSFSIRSRKDLKDIFDIYSVPCNRSASESAPLYTNLTIEENTNDLQPDLDLLTRNVSDLGLFMKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPNISMCHQGLLSFEGFARFLMDKDNFASKNDESRENKKDLQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQLASMQTQAFTGGNANPPPASNEEEEDEEDEYDYDYESLSDADVLTASPVPNSQEDNILEDRPENKSCADKLQFEYNEEVPKRIKKADNSSGNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNSSGSGRGKERKSRKSIFGNNPGRMSPGETASFNRTSGKSSCEGIRQIWEEPPLSPNTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERDLDAMDPATYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLKALKRGYRHLQLRNLHNEILEISSLFINSRRMEDNPSGSTRPASLMFNTEERKCSQTHKVTVHGVPGPEPFAVFTINEGTKAKQLLQQILAVDQDTKLTAADYFLMEEKHFISKEKNECRKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKNIVHDDREVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPCDYVLLEEVMKDAPNKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRRGISFASELKKLTKSTKQTRGLTSPPQLVASESVQSKEEKPMGALASGDTAGYQS

Target ORF information:

RefSeq Version	XM_017588656.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588656.1

ORF Insert Sequence:

ATGACGTCCG AAGAAATGGC CGCTTCCTTC CTCATCCCGG TGCCTCAAAG GAAAGTGGCT 
TCTGCCCAGT CAGTGGCAGA GGAGCGGGGT GAAAAGGTCT CAGAAGCCGG CATTCCTAAG 
ACACGAGCTG GCAGACAGGG TGGGCTCACC CCTCGTACCA TCTCACAGCG GAACGAACCT 
GAGGAAGAGT CTCCTAGAAC TGATTTTTCC CAGGTCTTCT CAATAGCGAG GGGGGAACTA 
GACAGCGACG AGAATCACAA TGAAAGGTGC TGGGAGGAAA ATGTGCCAGG TTCCACGAAA 
AACCACGCGG TTAACTGCAA CAGCTTATTG CAAAGCCACC AGCATGCGCT TCCCCCGAGC 
CAGCTCTGTG AAGTCTGTGA CTCTGTCACA GAAGAACACC TGTGTTTGCA GCCTGGGATT 
CCTTCTCCAC TGGAAAGGAA GGTGTTTCCT GGGATCGAAC TGGAGATGGA GGACTCTCCC 
ATGGACGTGA GCCCTTTGGG AAACCAGCCT GGGATCATGG AGTCCAGCGG ACCTCACTCT 
GACCGCAACA TGGCGGTATT TCATTTCCAT TATGCAGGTG ACAGGACAAT GCCAGGTGCT 
TTCCATACCC TGTCAGAAAA ATTCATTTTG GATGACTGTG CAAACTGTGT TACTCTTCCT 
GGGGGGCAGC AAAACAAAAA TTACATGGCG TATACTTGCA AACTGGTGGA GTTGACAAGA 
ACCTGTGGTA GTAAGAATGG GCAGCTGAAG TGTGACCACT GCACCTCGCT GCGGGATGAA 
TACCTGTGCT TTGAAAGCTC CTGCCGGAAG GCTGAGGCTC TCTCCTCAGG TGGTGGCTTT 
TGCGAGGACG GATTTACTCA TGGTCCCTCT GCCAAGACTT TTCTGAACCC TTTGGAGGAA 
TTCTCTGATA ATTGTGAAGA CGTGGACGAT ATTTTTAAAG GCAAAAAGGA GCGGTCCACT 
TTGTTAGTCC GAAGGTTTTG TAAAAATGAC AGGGAAGTTA AGAAGTCTGT GTATACTGGG 
ACAAGGGCCA TTGTGAGAAC CCTGCCTTCT GGCCACATTG GGCTGGCTGC TTGGAGTTAC 
GTCGATCAGA AGAAAGCTGG TCTCATGTGG CCTTGTGGGA ATGGAATGAG ACCTCTGTCC 
ACAGTGGACG TAAGGCAAAG TGGGAGACAG CGTCTGTCTG AAGCCCAGTG GTGTCTGATC 
TACAGTGCCG TGAGAAGAGA AGAGACAGAA GATACGGTTG GCTCTCTTCT GCATTGCTCC 
ACACAGCTCC CAACCCCTGA CACAGCCCAT GGAAGGATCG GAGATGGTCC CTGCCTAAAG 
CAATGTGTGC GAGACAGCGA GTGTGAGTAC CGTGCCACCC TCCAAAGGAC GTCCATAGCT 
CAGTACATCA CCGGTTCTCT CCTAGAGGCA ACCACATCTT TAGGAGCAAG AAGCAGCCTT 
CTCAGTTCCT TTGGAGGATC CACTGGGCGA ATAATGCTGA AAGAACGCCA ACCGGGCACT 
TCTATGGCCA ATTCCAGTCC TGTTCCTTCA AGTTCAGCTG GAATCAGCAA GGAGCTGATT 
GATCTCCAGC CCCTCATTCA GTTCCCGGAG GAAGTAGCCA GCATCTTAAC AGAACAGGAA 
CAGAACATTT ACCGCAGGGT CCTGCCCATG GACTACCTCT GTTTCTTAAC CCGGGACTTG 
AGTTCCCCCG AGTGTCAGAG ATCACTGCCC CGTCTCAAAG CATGCATCTC AGAATCAATC 
CTCATGTCTC AAAGTGGAGA GCACAATGCC CTTGAAGACC TCGTGATGAG ATTTAACGAG 
GTGAGCTCCT GGGTAACATG GCTGATCCTC ACGGCAGGCT CCATGGAGGA GAAGCGGGAA 
GTCTTTTCTT ATTTGGTGCA TGTGGCCAAA TGCTGCTGGA ACATGGGCAA CTACAATGCT 
GTCATGGAGT TCCTGGCTGG CCTCAGGTCA AGGAAAGTTC TGAAGATGTG GCAGTTCATG 
GACCAGTCTG ACATCGAGAC CATGAGGAGC CTGAAAGACG CCATGGCTCA GCATGAGTCT 
TCCGTGGAGT ACAAGAAGGT AGTGACCCGA GCCTTGCACA TCCCCGGCTG CAAAGTGGTT 
CCTTTCTGTG GGGTGTTCCT GAAGGAGCTC TGTGAGGTGC TTGATGGTGC CTCTGGCCTC 
CTGAAGCTCT GCCCACGATA CAGCTCCCAA GAAGAAGCCC TGGAGTTTGT AGCGGATTAC 
AGTGGACAAG ATAACTTCTT ACAGCGAGTG GGGCAGAATG GCTTGAAGAA TCCAGAGAAG 
GAGCTCACGG TCAACAGCAT CTTTCAGATC ATCCGGAGCT GCAGCCGAAG CTTGGAGACG 
GAGGACGAGG AGAGCGCCAG CGAAGGGAGC GGCTCTAGGA AAAACTCCCT CAAGGACAAA 
ACCCGGTGGC AGTTTATAAT TGGAGATTTG CTGGATTCAG ACAATGACAT CTTTGAGAAG 
TCCAAAGAGT GCGACCCTCA TGGATCAGAG GAGTCACAGA AAGCCTTTGA CCATGGGACA 
GAGCTCATCC CATGGTATGT GCTGTCCATC CAAGCGGATG TGCACCAGTT CCTCCTGCAG 
GGGGCCACAG TCATCCACTA TGACCAGGAC ACACATCTCT CCGCCCGCTG CTTTCTCCAG 
TTGCAGCCTG ACAATAGTAC CTTGACCTGG ATGAAACCTC CCACTGCCTC CCCAGCTGGT 
GCCAGACTCA AGCTCGGTGT GCTTAGTAAC GTGGCCGAAC CTGGCAAGTT CCCATCACTG 
GGCAACGCTG GGGTGAGCGG CCTGGTAGAG GGAATCCTGG ATCTGTTTTC AGTGAAGGCT 
GTGTACATGG GACACCCTGG CATTGATATA CACACTGTGT GTGTCCAGAA CAAACTTAGT 
AGCATGCTTC TGTCAGAGAC TGGGGTGACG CTGCTCTATG GGCTTCAGAC CACCGACAAT 
AGATTACTGC ACTTCGTGGC ACCAAAGCAC ACAGCTAAAA TGCTTTTCAG TGGATTGCTG 
GAACTCACTA CAGCTGTGAG AAAGATACGG AAGTTCCCTG ACCAAAGACA ACAGTGGCTT 
CGGAAACAGT ATGTCAGCTT CTATCAGGAG GATGGTCGGT ATGAAGGCCC AACCTTGGCA 
CATGCTGTGG AGTTGTTTGG TGGTAGGAGA TGGAGCACTA GAAACCCCAG CCCTGGGATG 
TCAGCAAAGA ATGCCGAGAA GCCCAACATG CAGAGAAACA ACACCCTAGG CATAAGCACC 
ACCAAGAAAA AGAAGAAAAT GCTCATGAGG GGGGAGAGTG GAGAGGTGAC GGACGATGAG 
ATGGCCACCC GCAAGGCCAA AATGTACAGA GAGTGTCGCA GCCGGAGCGG CTCTGATCCT 
CAAGAGGCTA ATGAACAGGA AGACTCAGAG GCCAATGTCA TCACTAACCC TCCCAACCCC 
CTCCATTCCA GAAGAGCCTA CTCATTGACC ACGGCTGGGT CCCCCAACTT GGCTACCGGG 
ATGTCATCTC CTATCAGTGC CTGGAGTAGC AGCAGCTGGC ACGGGCGGAT CAAAGGAGGA 
ATGAAAGGCT TCCAGAGCTT CATGGTCTCA GACAGTAACA TGAGTTTCAT TGAATTTGTC 
GAGCTGTTCA AATCATTCAG CATAAGGAGC CGCAAGGACT TGAAGGACAT CTTTGACATC 
TACTCTGTAC CCTGCAATCG GTCTGCTTCT GAGTCAGCCC CACTGTACAC CAACCTGACA 
ATCGAAGAAA ATACCAATGA CCTTCAGCCT GACCTAGATT TGCTGACTAG AAACGTCTCC 
GACTTGGGGC TGTTCATGAA GAGTAAGCAG CAGCTTTCTG ACAACCAGAG GCAGATATCT 
GACGCCATTG CTGCTGCAAG CATTGTGACT AATGGCACTG GGATTGAAAG CACGTCCCTG 
GGCATATTTG GGGTCGGCAT ACTCCAGCTC AACGACTTCC TGGTGAATTG CCAAGGAGAA 
CACTGCACAT ATGATGAAAT CCTCAGCATC ATCCAGAAGT TCGAGCCCAA CATCAGCATG 
TGTCATCAAG GTCTATTGTC CTTCGAAGGG TTTGCAAGGT TTCTGATGGA TAAAGACAAT 
TTCGCCTCAA AAAATGACGA GTCGCGGGAG AACAAGAAAG ACCTGCAGCT ACCCCTCTCC 
TACTATTACA TCGAATCTTC GCACAACACC TACCTCACCG GCCATCAGCT CAAGGGGGAG 
TCCTCTGTGG AGCTCTACAG CCAGGTCCTC TTGCAAGGAT GCAGAAGCAT AGAGCTGGAC 
TGCTGGGATG GAGATGATGG GATGCCCATT ATTTATCATG GACATACGCT GACAACCAAG 
ATCCCCTTCA AGGAAGTGGT GGAAGCCATC GATCGCAGTG CCTTCATCAC CTCCGACCTG 
CCAATCATCA TATCCATTGA GAACCATTGC TCATTGCCTC AGCAACGGAA AATGGCAGAA 
ATTTTCAAGA GTGTGTTTGG AGAAAAGCTG GTGGCTAAGT TCTTGTTCGA GACCGATTTC 
TCAGATGACC CAATGCTTCC CTCACCTGAC CAACTTAGAA GGAAAGTGCT TCTTAAAAAC 
AAGAAGCTAA AGGCCCATCA GACACCTGTC GACATCCTCA AACAAAAGGC TCATCAGTTA 
GCATCCATGC AAACACAGGC TTTCACTGGT GGGAATGCCA ACCCGCCACC TGCCAGTAAT 
GAGGAAGAGG AAGACGAGGA GGACGAATAT GATTATGATT ACGAGTCCCT GTCTGATGCA 
GATGTCCTTA CTGCTTCCCC TGTTCCTAAC AGTCAGGAAG ACAACATTCT GGAAGACAGA 
CCTGAGAACA AATCATGTGC CGACAAGCTT CAGTTTGAGT ACAATGAAGA AGTCCCCAAG 
AGGATAAAGA AAGCAGACAA CTCCTCCGGC AATAAGGGAA AGGTCTATGA CATGGAACTG 
GGAGAGGAGT TTTACCTTCC TCAGAACAAA AAAGAAAGCA GGCAGATTGC ACCGGAGCTC 
TCTGACCTCG TCATCTATTG CCAGGCGGTA AAATTTCCAG GCCTGTCAAC TCTAAATTCA 
TCTGGCTCTG GCAGAGGGAA AGAAAGGAAA AGCAGGAAGT CCATTTTTGG CAACAATCCC 
GGCAGAATGA GCCCAGGGGA GACAGCGTCA TTTAACAGAA CATCTGGAAA AAGTTCTTGT 
GAAGGAATTC GACAAATCTG GGAGGAACCT CCACTCAGCC CAAACACATC CCTCAGCGCC 
ATCATTAGAA CTCCTAAATG CTATCATATC TCGTCACTGA ATGAAAATGC CGCCAAACGT 
CTGTGTCGCA GGTATTCTCA GAAACTGATC CAGCACACTG CTTGCCAGCT GCTGAGGACC 
TACCCGGCAG CGACCAGAAT CGACTCATCC AATCCCAACC CCCTCATGTT CTGGCTCCAT 
GGGATACAGC TTGTAGCTCT AAACTACCAG ACGGATGACC TTCCTTTACA TTTAAACGCT 
GCCATGTTTG AGGCGAATGG TGGGTGTGGC TATGTTTTGA AGCCCCCTGT CCTGTGGGAT 
AAAAGCTGTC CCATGTATCA GAAGTTTTCA CCACTGGAAA GAGACCTGGA CGCCATGGAT 
CCTGCTACCT ATTCTTTAAC CATCATCTCT GGCCAGAATG TGTGTCCTAG TAACAGCACC 
GGAAGTCCGT GCATTGAAGT TGACGTCTTG GGCATGCCCC TGGACAGCTG CCATTTCCGC 
ACAAAACCCA TCCACCGAAA CACTCTGAAC CCCATGTGGA ATGAGCAGTT TCTCTTCCGG 
GTTCACTTTG AAGATCTTGT ATTTCTTCGT TTTGCAGTTG TGGAGAACAA CAGTTCGGCG 
ATAACGGCTC AGAGAATCAT TCCACTCAAA GCTTTAAAGA GAGGATATCG GCATCTTCAG 
CTGCGGAACC TCCACAACGA GATCTTGGAA ATCTCTAGCT TATTCATAAA CAGCAGGAGG 
ATGGAAGATA ATCCCTCTGG CAGTACCCGG CCAGCCTCTT TGATGTTCAA TACTGAAGAG 
AGGAAATGTT CTCAGACTCA CAAAGTCACA GTGCATGGTG TCCCAGGTCC AGAGCCCTTT 
GCTGTTTTCA CAATAAACGA AGGCACCAAG GCAAAGCAGC TTCTCCAACA GATTTTGGCA 
GTTGACCAGG ACACCAAACT TACAGCCGCA GACTATTTTC TGATGGAAGA GAAGCATTTT 
ATATCTAAGG AAAAGAATGA ATGCAGGAAA CAACCATTCC AGAGAGCTGT GGGCCCAGAG 
GAAGATATTG TGCAGATTTT AAACAGCTGG TTCCCAGAAG AAGGCTACGT AGGCAGGATT 
GTCTTAAAGC CCCAACAAGA AACCCTAGAA GAGAAAAACA TTGTCCACGA TGACAGAGAG 
GTGATCTTGA GCTCAGAAGA GGAGAGTTTC TTTGTCCAAG TGCACGATGT CTCTCCAGAG 
CAGCCTCGAA CTGTCATCAA AGCCCCACGT GTCAGCACTG CACAGGATGT GATTCAGCAG 
ACCTTGTGCA AAGCCAAATA TTCCTACAGC ATCCTGAACA ACCCCAATCC GTGTGACTAT 
GTGCTTTTGG AAGAGGTAAT GAAAGATGCA CCCAATAAGA AGTCTTCTAC CCCGAAGTCC 
TCACAGAGGA TCCTCTTGGA CCAAGAATGT GTGTTTCAAG CCCAAAGCAA GTGGAAGGGT 
GCAGGGAAAT TCATCCTTAA GCTAAAGGAG CAGGTGCAGG CATCCAGAGA AGATAAAAGG 
AGGGGCATCT CCTTTGCAAG TGAACTCAAG AAACTCACGA AGTCCACTAA GCAAACCCGA 
GGACTCACCT CGCCTCCTCA GCTTGTGGCC TCAGAAAGTG TGCAAAGCAA GGAGGAGAAA 
CCCATGGGTG CCTTGGCCTC TGGTGACACA GCGGGCTACC AGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plce1 ( XM_017588657.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444146.1 Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X2, mRNA.

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CloneID	ORa56295
Clone ID Related Accession (Same CDS sequence)	XM_017588656.1 , XM_017588657.1 , XM_017588658.1
Accession Version	XM_017588657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCCG AAGAAATGGC CGCTTCCTTC CTCATCCCGG TGCCTCAAAG GAAAGTGGCT 
TCTGCCCAGT CAGTGGCAGA GGAGCGGGGT GAAAAGGTCT CAGAAGCCGG CATTCCTAAG 
ACACGAGCTG GCAGACAGGG TGGGCTCACC CCTCGTACCA TCTCACAGCG GAACGAACCT 
GAGGAAGAGT CTCCTAGAAC TGATTTTTCC CAGGTCTTCT CAATAGCGAG GGGGGAACTA 
GACAGCGACG AGAATCACAA TGAAAGGTGC TGGGAGGAAA ATGTGCCAGG TTCCACGAAA 
AACCACGCGG TTAACTGCAA CAGCTTATTG CAAAGCCACC AGCATGCGCT TCCCCCGAGC 
CAGCTCTGTG AAGTCTGTGA CTCTGTCACA GAAGAACACC TGTGTTTGCA GCCTGGGATT 
CCTTCTCCAC TGGAAAGGAA GGTGTTTCCT GGGATCGAAC TGGAGATGGA GGACTCTCCC 
ATGGACGTGA GCCCTTTGGG AAACCAGCCT GGGATCATGG AGTCCAGCGG ACCTCACTCT 
GACCGCAACA TGGCGGTATT TCATTTCCAT TATGCAGGTG ACAGGACAAT GCCAGGTGCT 
TTCCATACCC TGTCAGAAAA ATTCATTTTG GATGACTGTG CAAACTGTGT TACTCTTCCT 
GGGGGGCAGC AAAACAAAAA TTACATGGCG TATACTTGCA AACTGGTGGA GTTGACAAGA 
ACCTGTGGTA GTAAGAATGG GCAGCTGAAG TGTGACCACT GCACCTCGCT GCGGGATGAA 
TACCTGTGCT TTGAAAGCTC CTGCCGGAAG GCTGAGGCTC TCTCCTCAGG TGGTGGCTTT 
TGCGAGGACG GATTTACTCA TGGTCCCTCT GCCAAGACTT TTCTGAACCC TTTGGAGGAA 
TTCTCTGATA ATTGTGAAGA CGTGGACGAT ATTTTTAAAG GCAAAAAGGA GCGGTCCACT 
TTGTTAGTCC GAAGGTTTTG TAAAAATGAC AGGGAAGTTA AGAAGTCTGT GTATACTGGG 
ACAAGGGCCA TTGTGAGAAC CCTGCCTTCT GGCCACATTG GGCTGGCTGC TTGGAGTTAC 
GTCGATCAGA AGAAAGCTGG TCTCATGTGG CCTTGTGGGA ATGGAATGAG ACCTCTGTCC 
ACAGTGGACG TAAGGCAAAG TGGGAGACAG CGTCTGTCTG AAGCCCAGTG GTGTCTGATC 
TACAGTGCCG TGAGAAGAGA AGAGACAGAA GATACGGTTG GCTCTCTTCT GCATTGCTCC 
ACACAGCTCC CAACCCCTGA CACAGCCCAT GGAAGGATCG GAGATGGTCC CTGCCTAAAG 
CAATGTGTGC GAGACAGCGA GTGTGAGTAC CGTGCCACCC TCCAAAGGAC GTCCATAGCT 
CAGTACATCA CCGGTTCTCT CCTAGAGGCA ACCACATCTT TAGGAGCAAG AAGCAGCCTT 
CTCAGTTCCT TTGGAGGATC CACTGGGCGA ATAATGCTGA AAGAACGCCA ACCGGGCACT 
TCTATGGCCA ATTCCAGTCC TGTTCCTTCA AGTTCAGCTG GAATCAGCAA GGAGCTGATT 
GATCTCCAGC CCCTCATTCA GTTCCCGGAG GAAGTAGCCA GCATCTTAAC AGAACAGGAA 
CAGAACATTT ACCGCAGGGT CCTGCCCATG GACTACCTCT GTTTCTTAAC CCGGGACTTG 
AGTTCCCCCG AGTGTCAGAG ATCACTGCCC CGTCTCAAAG CATGCATCTC AGAATCAATC 
CTCATGTCTC AAAGTGGAGA GCACAATGCC CTTGAAGACC TCGTGATGAG ATTTAACGAG 
GTGAGCTCCT GGGTAACATG GCTGATCCTC ACGGCAGGCT CCATGGAGGA GAAGCGGGAA 
GTCTTTTCTT ATTTGGTGCA TGTGGCCAAA TGCTGCTGGA ACATGGGCAA CTACAATGCT 
GTCATGGAGT TCCTGGCTGG CCTCAGGTCA AGGAAAGTTC TGAAGATGTG GCAGTTCATG 
GACCAGTCTG ACATCGAGAC CATGAGGAGC CTGAAAGACG CCATGGCTCA GCATGAGTCT 
TCCGTGGAGT ACAAGAAGGT AGTGACCCGA GCCTTGCACA TCCCCGGCTG CAAAGTGGTT 
CCTTTCTGTG GGGTGTTCCT GAAGGAGCTC TGTGAGGTGC TTGATGGTGC CTCTGGCCTC 
CTGAAGCTCT GCCCACGATA CAGCTCCCAA GAAGAAGCCC TGGAGTTTGT AGCGGATTAC 
AGTGGACAAG ATAACTTCTT ACAGCGAGTG GGGCAGAATG GCTTGAAGAA TCCAGAGAAG 
GAGCTCACGG TCAACAGCAT CTTTCAGATC ATCCGGAGCT GCAGCCGAAG CTTGGAGACG 
GAGGACGAGG AGAGCGCCAG CGAAGGGAGC GGCTCTAGGA AAAACTCCCT CAAGGACAAA 
ACCCGGTGGC AGTTTATAAT TGGAGATTTG CTGGATTCAG ACAATGACAT CTTTGAGAAG 
TCCAAAGAGT GCGACCCTCA TGGATCAGAG GAGTCACAGA AAGCCTTTGA CCATGGGACA 
GAGCTCATCC CATGGTATGT GCTGTCCATC CAAGCGGATG TGCACCAGTT CCTCCTGCAG 
GGGGCCACAG TCATCCACTA TGACCAGGAC ACACATCTCT CCGCCCGCTG CTTTCTCCAG 
TTGCAGCCTG ACAATAGTAC CTTGACCTGG ATGAAACCTC CCACTGCCTC CCCAGCTGGT 
GCCAGACTCA AGCTCGGTGT GCTTAGTAAC GTGGCCGAAC CTGGCAAGTT CCCATCACTG 
GGCAACGCTG GGGTGAGCGG CCTGGTAGAG GGAATCCTGG ATCTGTTTTC AGTGAAGGCT 
GTGTACATGG GACACCCTGG CATTGATATA CACACTGTGT GTGTCCAGAA CAAACTTAGT 
AGCATGCTTC TGTCAGAGAC TGGGGTGACG CTGCTCTATG GGCTTCAGAC CACCGACAAT 
AGATTACTGC ACTTCGTGGC ACCAAAGCAC ACAGCTAAAA TGCTTTTCAG TGGATTGCTG 
GAACTCACTA CAGCTGTGAG AAAGATACGG AAGTTCCCTG ACCAAAGACA ACAGTGGCTT 
CGGAAACAGT ATGTCAGCTT CTATCAGGAG GATGGTCGGT ATGAAGGCCC AACCTTGGCA 
CATGCTGTGG AGTTGTTTGG TGGTAGGAGA TGGAGCACTA GAAACCCCAG CCCTGGGATG 
TCAGCAAAGA ATGCCGAGAA GCCCAACATG CAGAGAAACA ACACCCTAGG CATAAGCACC 
ACCAAGAAAA AGAAGAAAAT GCTCATGAGG GGGGAGAGTG GAGAGGTGAC GGACGATGAG 
ATGGCCACCC GCAAGGCCAA AATGTACAGA GAGTGTCGCA GCCGGAGCGG CTCTGATCCT 
CAAGAGGCTA ATGAACAGGA AGACTCAGAG GCCAATGTCA TCACTAACCC TCCCAACCCC 
CTCCATTCCA GAAGAGCCTA CTCATTGACC ACGGCTGGGT CCCCCAACTT GGCTACCGGG 
ATGTCATCTC CTATCAGTGC CTGGAGTAGC AGCAGCTGGC ACGGGCGGAT CAAAGGAGGA 
ATGAAAGGCT TCCAGAGCTT CATGGTCTCA GACAGTAACA TGAGTTTCAT TGAATTTGTC 
GAGCTGTTCA AATCATTCAG CATAAGGAGC CGCAAGGACT TGAAGGACAT CTTTGACATC 
TACTCTGTAC CCTGCAATCG GTCTGCTTCT GAGTCAGCCC CACTGTACAC CAACCTGACA 
ATCGAAGAAA ATACCAATGA CCTTCAGCCT GACCTAGATT TGCTGACTAG AAACGTCTCC 
GACTTGGGGC TGTTCATGAA GAGTAAGCAG CAGCTTTCTG ACAACCAGAG GCAGATATCT 
GACGCCATTG CTGCTGCAAG CATTGTGACT AATGGCACTG GGATTGAAAG CACGTCCCTG 
GGCATATTTG GGGTCGGCAT ACTCCAGCTC AACGACTTCC TGGTGAATTG CCAAGGAGAA 
CACTGCACAT ATGATGAAAT CCTCAGCATC ATCCAGAAGT TCGAGCCCAA CATCAGCATG 
TGTCATCAAG GTCTATTGTC CTTCGAAGGG TTTGCAAGGT TTCTGATGGA TAAAGACAAT 
TTCGCCTCAA AAAATGACGA GTCGCGGGAG AACAAGAAAG ACCTGCAGCT ACCCCTCTCC 
TACTATTACA TCGAATCTTC GCACAACACC TACCTCACCG GCCATCAGCT CAAGGGGGAG 
TCCTCTGTGG AGCTCTACAG CCAGGTCCTC TTGCAAGGAT GCAGAAGCAT AGAGCTGGAC 
TGCTGGGATG GAGATGATGG GATGCCCATT ATTTATCATG GACATACGCT GACAACCAAG 
ATCCCCTTCA AGGAAGTGGT GGAAGCCATC GATCGCAGTG CCTTCATCAC CTCCGACCTG 
CCAATCATCA TATCCATTGA GAACCATTGC TCATTGCCTC AGCAACGGAA AATGGCAGAA 
ATTTTCAAGA GTGTGTTTGG AGAAAAGCTG GTGGCTAAGT TCTTGTTCGA GACCGATTTC 
TCAGATGACC CAATGCTTCC CTCACCTGAC CAACTTAGAA GGAAAGTGCT TCTTAAAAAC 
AAGAAGCTAA AGGCCCATCA GACACCTGTC GACATCCTCA AACAAAAGGC TCATCAGTTA 
GCATCCATGC AAACACAGGC TTTCACTGGT GGGAATGCCA ACCCGCCACC TGCCAGTAAT 
GAGGAAGAGG AAGACGAGGA GGACGAATAT GATTATGATT ACGAGTCCCT GTCTGATGCA 
GATGTCCTTA CTGCTTCCCC TGTTCCTAAC AGTCAGGAAG ACAACATTCT GGAAGACAGA 
CCTGAGAACA AATCATGTGC CGACAAGCTT CAGTTTGAGT ACAATGAAGA AGTCCCCAAG 
AGGATAAAGA AAGCAGACAA CTCCTCCGGC AATAAGGGAA AGGTCTATGA CATGGAACTG 
GGAGAGGAGT TTTACCTTCC TCAGAACAAA AAAGAAAGCA GGCAGATTGC ACCGGAGCTC 
TCTGACCTCG TCATCTATTG CCAGGCGGTA AAATTTCCAG GCCTGTCAAC TCTAAATTCA 
TCTGGCTCTG GCAGAGGGAA AGAAAGGAAA AGCAGGAAGT CCATTTTTGG CAACAATCCC 
GGCAGAATGA GCCCAGGGGA GACAGCGTCA TTTAACAGAA CATCTGGAAA AAGTTCTTGT 
GAAGGAATTC GACAAATCTG GGAGGAACCT CCACTCAGCC CAAACACATC CCTCAGCGCC 
ATCATTAGAA CTCCTAAATG CTATCATATC TCGTCACTGA ATGAAAATGC CGCCAAACGT 
CTGTGTCGCA GGTATTCTCA GAAACTGATC CAGCACACTG CTTGCCAGCT GCTGAGGACC 
TACCCGGCAG CGACCAGAAT CGACTCATCC AATCCCAACC CCCTCATGTT CTGGCTCCAT 
GGGATACAGC TTGTAGCTCT AAACTACCAG ACGGATGACC TTCCTTTACA TTTAAACGCT 
GCCATGTTTG AGGCGAATGG TGGGTGTGGC TATGTTTTGA AGCCCCCTGT CCTGTGGGAT 
AAAAGCTGTC CCATGTATCA GAAGTTTTCA CCACTGGAAA GAGACCTGGA CGCCATGGAT 
CCTGCTACCT ATTCTTTAAC CATCATCTCT GGCCAGAATG TGTGTCCTAG TAACAGCACC 
GGAAGTCCGT GCATTGAAGT TGACGTCTTG GGCATGCCCC TGGACAGCTG CCATTTCCGC 
ACAAAACCCA TCCACCGAAA CACTCTGAAC CCCATGTGGA ATGAGCAGTT TCTCTTCCGG 
GTTCACTTTG AAGATCTTGT ATTTCTTCGT TTTGCAGTTG TGGAGAACAA CAGTTCGGCG 
ATAACGGCTC AGAGAATCAT TCCACTCAAA GCTTTAAAGA GAGGATATCG GCATCTTCAG 
CTGCGGAACC TCCACAACGA GATCTTGGAA ATCTCTAGCT TATTCATAAA CAGCAGGAGG 
ATGGAAGATA ATCCCTCTGG CAGTACCCGG CCAGCCTCTT TGATGTTCAA TACTGAAGAG 
AGGAAATGTT CTCAGACTCA CAAAGTCACA GTGCATGGTG TCCCAGGTCC AGAGCCCTTT 
GCTGTTTTCA CAATAAACGA AGGCACCAAG GCAAAGCAGC TTCTCCAACA GATTTTGGCA 
GTTGACCAGG ACACCAAACT TACAGCCGCA GACTATTTTC TGATGGAAGA GAAGCATTTT 
ATATCTAAGG AAAAGAATGA ATGCAGGAAA CAACCATTCC AGAGAGCTGT GGGCCCAGAG 
GAAGATATTG TGCAGATTTT AAACAGCTGG TTCCCAGAAG AAGGCTACGT AGGCAGGATT 
GTCTTAAAGC CCCAACAAGA AACCCTAGAA GAGAAAAACA TTGTCCACGA TGACAGAGAG 
GTGATCTTGA GCTCAGAAGA GGAGAGTTTC TTTGTCCAAG TGCACGATGT CTCTCCAGAG 
CAGCCTCGAA CTGTCATCAA AGCCCCACGT GTCAGCACTG CACAGGATGT GATTCAGCAG 
ACCTTGTGCA AAGCCAAATA TTCCTACAGC ATCCTGAACA ACCCCAATCC GTGTGACTAT 
GTGCTTTTGG AAGAGGTAAT GAAAGATGCA CCCAATAAGA AGTCTTCTAC CCCGAAGTCC 
TCACAGAGGA TCCTCTTGGA CCAAGAATGT GTGTTTCAAG CCCAAAGCAA GTGGAAGGGT 
GCAGGGAAAT TCATCCTTAA GCTAAAGGAG CAGGTGCAGG CATCCAGAGA AGATAAAAGG 
AGGGGCATCT CCTTTGCAAG TGAACTCAAG AAACTCACGA AGTCCACTAA GCAAACCCGA 
GGACTCACCT CGCCTCCTCA GCTTGTGGCC TCAGAAAGTG TGCAAAGCAA GGAGGAGAAA 
CCCATGGGTG CCTTGGCCTC TGGTGACACA GCGGGCTACC AGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444146.1
CDS	507..7394
Translation	MTSEEMAASFLIPVPQRKVASAQSVAEERGEKVSEAGIPKTRAGRQGGLTPRTISQRNEPEEESPRTDFSQVFSIARGELDSDENHNERCWEENVPGSTKNHAVNCNSLLQSHQHALPPSQLCEVCDSVTEEHLCLQPGIPSPLERKVFPGIELEMEDSPMDVSPLGNQPGIMESSGPHSDRNMAVFHFHYAGDRTMPGAFHTLSEKFILDDCANCVTLPGGQQNKNYMAYTCKLVELTRTCGSKNGQLKCDHCTSLRDEYLCFESSCRKAEALSSGGGFCEDGFTHGPSAKTFLNPLEEFSDNCEDVDDIFKGKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLAAWSYVDQKKAGLMWPCGNGMRPLSTVDVRQSGRQRLSEAQWCLIYSAVRREETEDTVGSLLHCSTQLPTPDTAHGRIGDGPCLKQCVRDSECEYRATLQRTSIAQYITGSLLEATTSLGARSSLLSSFGGSTGRIMLKERQPGTSMANSSPVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRRVLPMDYLCFLTRDLSSPECQRSLPRLKACISESILMSQSGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSVEYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEEALEFVADYSGQDNFLQRVGQNGLKNPEKELTVNSIFQIIRSCSRSLETEDEESASEGSGSRKNSLKDKTRWQFIIGDLLDSDNDIFEKSKECDPHGSEESQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTASPAGARLKLGVLSNVAEPGKFPSLGNAGVSGLVEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSMLLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTTAVRKIRKFPDQRQQWLRKQYVSFYQEDGRYEGPTLAHAVELFGGRRWSTRNPSPGMSAKNAEKPNMQRNNTLGISTTKKKKKMLMRGESGEVTDDEMATRKAKMYRECRSRSGSDPQEANEQEDSEANVITNPPNPLHSRRAYSLTTAGSPNLATGMSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFIEFVELFKSFSIRSRKDLKDIFDIYSVPCNRSASESAPLYTNLTIEENTNDLQPDLDLLTRNVSDLGLFMKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPNISMCHQGLLSFEGFARFLMDKDNFASKNDESRENKKDLQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQLASMQTQAFTGGNANPPPASNEEEEDEEDEYDYDYESLSDADVLTASPVPNSQEDNILEDRPENKSCADKLQFEYNEEVPKRIKKADNSSGNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNSSGSGRGKERKSRKSIFGNNPGRMSPGETASFNRTSGKSSCEGIRQIWEEPPLSPNTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERDLDAMDPATYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLKALKRGYRHLQLRNLHNEILEISSLFINSRRMEDNPSGSTRPASLMFNTEERKCSQTHKVTVHGVPGPEPFAVFTINEGTKAKQLLQQILAVDQDTKLTAADYFLMEEKHFISKEKNECRKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKNIVHDDREVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPCDYVLLEEVMKDAPNKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRRGISFASELKKLTKSTKQTRGLTSPPQLVASESVQSKEEKPMGALASGDTAGYQS

Target ORF information:

RefSeq Version	XM_017588657.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588657.1

ORF Insert Sequence:

ATGACGTCCG AAGAAATGGC CGCTTCCTTC CTCATCCCGG TGCCTCAAAG GAAAGTGGCT 
TCTGCCCAGT CAGTGGCAGA GGAGCGGGGT GAAAAGGTCT CAGAAGCCGG CATTCCTAAG 
ACACGAGCTG GCAGACAGGG TGGGCTCACC CCTCGTACCA TCTCACAGCG GAACGAACCT 
GAGGAAGAGT CTCCTAGAAC TGATTTTTCC CAGGTCTTCT CAATAGCGAG GGGGGAACTA 
GACAGCGACG AGAATCACAA TGAAAGGTGC TGGGAGGAAA ATGTGCCAGG TTCCACGAAA 
AACCACGCGG TTAACTGCAA CAGCTTATTG CAAAGCCACC AGCATGCGCT TCCCCCGAGC 
CAGCTCTGTG AAGTCTGTGA CTCTGTCACA GAAGAACACC TGTGTTTGCA GCCTGGGATT 
CCTTCTCCAC TGGAAAGGAA GGTGTTTCCT GGGATCGAAC TGGAGATGGA GGACTCTCCC 
ATGGACGTGA GCCCTTTGGG AAACCAGCCT GGGATCATGG AGTCCAGCGG ACCTCACTCT 
GACCGCAACA TGGCGGTATT TCATTTCCAT TATGCAGGTG ACAGGACAAT GCCAGGTGCT 
TTCCATACCC TGTCAGAAAA ATTCATTTTG GATGACTGTG CAAACTGTGT TACTCTTCCT 
GGGGGGCAGC AAAACAAAAA TTACATGGCG TATACTTGCA AACTGGTGGA GTTGACAAGA 
ACCTGTGGTA GTAAGAATGG GCAGCTGAAG TGTGACCACT GCACCTCGCT GCGGGATGAA 
TACCTGTGCT TTGAAAGCTC CTGCCGGAAG GCTGAGGCTC TCTCCTCAGG TGGTGGCTTT 
TGCGAGGACG GATTTACTCA TGGTCCCTCT GCCAAGACTT TTCTGAACCC TTTGGAGGAA 
TTCTCTGATA ATTGTGAAGA CGTGGACGAT ATTTTTAAAG GCAAAAAGGA GCGGTCCACT 
TTGTTAGTCC GAAGGTTTTG TAAAAATGAC AGGGAAGTTA AGAAGTCTGT GTATACTGGG 
ACAAGGGCCA TTGTGAGAAC CCTGCCTTCT GGCCACATTG GGCTGGCTGC TTGGAGTTAC 
GTCGATCAGA AGAAAGCTGG TCTCATGTGG CCTTGTGGGA ATGGAATGAG ACCTCTGTCC 
ACAGTGGACG TAAGGCAAAG TGGGAGACAG CGTCTGTCTG AAGCCCAGTG GTGTCTGATC 
TACAGTGCCG TGAGAAGAGA AGAGACAGAA GATACGGTTG GCTCTCTTCT GCATTGCTCC 
ACACAGCTCC CAACCCCTGA CACAGCCCAT GGAAGGATCG GAGATGGTCC CTGCCTAAAG 
CAATGTGTGC GAGACAGCGA GTGTGAGTAC CGTGCCACCC TCCAAAGGAC GTCCATAGCT 
CAGTACATCA CCGGTTCTCT CCTAGAGGCA ACCACATCTT TAGGAGCAAG AAGCAGCCTT 
CTCAGTTCCT TTGGAGGATC CACTGGGCGA ATAATGCTGA AAGAACGCCA ACCGGGCACT 
TCTATGGCCA ATTCCAGTCC TGTTCCTTCA AGTTCAGCTG GAATCAGCAA GGAGCTGATT 
GATCTCCAGC CCCTCATTCA GTTCCCGGAG GAAGTAGCCA GCATCTTAAC AGAACAGGAA 
CAGAACATTT ACCGCAGGGT CCTGCCCATG GACTACCTCT GTTTCTTAAC CCGGGACTTG 
AGTTCCCCCG AGTGTCAGAG ATCACTGCCC CGTCTCAAAG CATGCATCTC AGAATCAATC 
CTCATGTCTC AAAGTGGAGA GCACAATGCC CTTGAAGACC TCGTGATGAG ATTTAACGAG 
GTGAGCTCCT GGGTAACATG GCTGATCCTC ACGGCAGGCT CCATGGAGGA GAAGCGGGAA 
GTCTTTTCTT ATTTGGTGCA TGTGGCCAAA TGCTGCTGGA ACATGGGCAA CTACAATGCT 
GTCATGGAGT TCCTGGCTGG CCTCAGGTCA AGGAAAGTTC TGAAGATGTG GCAGTTCATG 
GACCAGTCTG ACATCGAGAC CATGAGGAGC CTGAAAGACG CCATGGCTCA GCATGAGTCT 
TCCGTGGAGT ACAAGAAGGT AGTGACCCGA GCCTTGCACA TCCCCGGCTG CAAAGTGGTT 
CCTTTCTGTG GGGTGTTCCT GAAGGAGCTC TGTGAGGTGC TTGATGGTGC CTCTGGCCTC 
CTGAAGCTCT GCCCACGATA CAGCTCCCAA GAAGAAGCCC TGGAGTTTGT AGCGGATTAC 
AGTGGACAAG ATAACTTCTT ACAGCGAGTG GGGCAGAATG GCTTGAAGAA TCCAGAGAAG 
GAGCTCACGG TCAACAGCAT CTTTCAGATC ATCCGGAGCT GCAGCCGAAG CTTGGAGACG 
GAGGACGAGG AGAGCGCCAG CGAAGGGAGC GGCTCTAGGA AAAACTCCCT CAAGGACAAA 
ACCCGGTGGC AGTTTATAAT TGGAGATTTG CTGGATTCAG ACAATGACAT CTTTGAGAAG 
TCCAAAGAGT GCGACCCTCA TGGATCAGAG GAGTCACAGA AAGCCTTTGA CCATGGGACA 
GAGCTCATCC CATGGTATGT GCTGTCCATC CAAGCGGATG TGCACCAGTT CCTCCTGCAG 
GGGGCCACAG TCATCCACTA TGACCAGGAC ACACATCTCT CCGCCCGCTG CTTTCTCCAG 
TTGCAGCCTG ACAATAGTAC CTTGACCTGG ATGAAACCTC CCACTGCCTC CCCAGCTGGT 
GCCAGACTCA AGCTCGGTGT GCTTAGTAAC GTGGCCGAAC CTGGCAAGTT CCCATCACTG 
GGCAACGCTG GGGTGAGCGG CCTGGTAGAG GGAATCCTGG ATCTGTTTTC AGTGAAGGCT 
GTGTACATGG GACACCCTGG CATTGATATA CACACTGTGT GTGTCCAGAA CAAACTTAGT 
AGCATGCTTC TGTCAGAGAC TGGGGTGACG CTGCTCTATG GGCTTCAGAC CACCGACAAT 
AGATTACTGC ACTTCGTGGC ACCAAAGCAC ACAGCTAAAA TGCTTTTCAG TGGATTGCTG 
GAACTCACTA CAGCTGTGAG AAAGATACGG AAGTTCCCTG ACCAAAGACA ACAGTGGCTT 
CGGAAACAGT ATGTCAGCTT CTATCAGGAG GATGGTCGGT ATGAAGGCCC AACCTTGGCA 
CATGCTGTGG AGTTGTTTGG TGGTAGGAGA TGGAGCACTA GAAACCCCAG CCCTGGGATG 
TCAGCAAAGA ATGCCGAGAA GCCCAACATG CAGAGAAACA ACACCCTAGG CATAAGCACC 
ACCAAGAAAA AGAAGAAAAT GCTCATGAGG GGGGAGAGTG GAGAGGTGAC GGACGATGAG 
ATGGCCACCC GCAAGGCCAA AATGTACAGA GAGTGTCGCA GCCGGAGCGG CTCTGATCCT 
CAAGAGGCTA ATGAACAGGA AGACTCAGAG GCCAATGTCA TCACTAACCC TCCCAACCCC 
CTCCATTCCA GAAGAGCCTA CTCATTGACC ACGGCTGGGT CCCCCAACTT GGCTACCGGG 
ATGTCATCTC CTATCAGTGC CTGGAGTAGC AGCAGCTGGC ACGGGCGGAT CAAAGGAGGA 
ATGAAAGGCT TCCAGAGCTT CATGGTCTCA GACAGTAACA TGAGTTTCAT TGAATTTGTC 
GAGCTGTTCA AATCATTCAG CATAAGGAGC CGCAAGGACT TGAAGGACAT CTTTGACATC 
TACTCTGTAC CCTGCAATCG GTCTGCTTCT GAGTCAGCCC CACTGTACAC CAACCTGACA 
ATCGAAGAAA ATACCAATGA CCTTCAGCCT GACCTAGATT TGCTGACTAG AAACGTCTCC 
GACTTGGGGC TGTTCATGAA GAGTAAGCAG CAGCTTTCTG ACAACCAGAG GCAGATATCT 
GACGCCATTG CTGCTGCAAG CATTGTGACT AATGGCACTG GGATTGAAAG CACGTCCCTG 
GGCATATTTG GGGTCGGCAT ACTCCAGCTC AACGACTTCC TGGTGAATTG CCAAGGAGAA 
CACTGCACAT ATGATGAAAT CCTCAGCATC ATCCAGAAGT TCGAGCCCAA CATCAGCATG 
TGTCATCAAG GTCTATTGTC CTTCGAAGGG TTTGCAAGGT TTCTGATGGA TAAAGACAAT 
TTCGCCTCAA AAAATGACGA GTCGCGGGAG AACAAGAAAG ACCTGCAGCT ACCCCTCTCC 
TACTATTACA TCGAATCTTC GCACAACACC TACCTCACCG GCCATCAGCT CAAGGGGGAG 
TCCTCTGTGG AGCTCTACAG CCAGGTCCTC TTGCAAGGAT GCAGAAGCAT AGAGCTGGAC 
TGCTGGGATG GAGATGATGG GATGCCCATT ATTTATCATG GACATACGCT GACAACCAAG 
ATCCCCTTCA AGGAAGTGGT GGAAGCCATC GATCGCAGTG CCTTCATCAC CTCCGACCTG 
CCAATCATCA TATCCATTGA GAACCATTGC TCATTGCCTC AGCAACGGAA AATGGCAGAA 
ATTTTCAAGA GTGTGTTTGG AGAAAAGCTG GTGGCTAAGT TCTTGTTCGA GACCGATTTC 
TCAGATGACC CAATGCTTCC CTCACCTGAC CAACTTAGAA GGAAAGTGCT TCTTAAAAAC 
AAGAAGCTAA AGGCCCATCA GACACCTGTC GACATCCTCA AACAAAAGGC TCATCAGTTA 
GCATCCATGC AAACACAGGC TTTCACTGGT GGGAATGCCA ACCCGCCACC TGCCAGTAAT 
GAGGAAGAGG AAGACGAGGA GGACGAATAT GATTATGATT ACGAGTCCCT GTCTGATGCA 
GATGTCCTTA CTGCTTCCCC TGTTCCTAAC AGTCAGGAAG ACAACATTCT GGAAGACAGA 
CCTGAGAACA AATCATGTGC CGACAAGCTT CAGTTTGAGT ACAATGAAGA AGTCCCCAAG 
AGGATAAAGA AAGCAGACAA CTCCTCCGGC AATAAGGGAA AGGTCTATGA CATGGAACTG 
GGAGAGGAGT TTTACCTTCC TCAGAACAAA AAAGAAAGCA GGCAGATTGC ACCGGAGCTC 
TCTGACCTCG TCATCTATTG CCAGGCGGTA AAATTTCCAG GCCTGTCAAC TCTAAATTCA 
TCTGGCTCTG GCAGAGGGAA AGAAAGGAAA AGCAGGAAGT CCATTTTTGG CAACAATCCC 
GGCAGAATGA GCCCAGGGGA GACAGCGTCA TTTAACAGAA CATCTGGAAA AAGTTCTTGT 
GAAGGAATTC GACAAATCTG GGAGGAACCT CCACTCAGCC CAAACACATC CCTCAGCGCC 
ATCATTAGAA CTCCTAAATG CTATCATATC TCGTCACTGA ATGAAAATGC CGCCAAACGT 
CTGTGTCGCA GGTATTCTCA GAAACTGATC CAGCACACTG CTTGCCAGCT GCTGAGGACC 
TACCCGGCAG CGACCAGAAT CGACTCATCC AATCCCAACC CCCTCATGTT CTGGCTCCAT 
GGGATACAGC TTGTAGCTCT AAACTACCAG ACGGATGACC TTCCTTTACA TTTAAACGCT 
GCCATGTTTG AGGCGAATGG TGGGTGTGGC TATGTTTTGA AGCCCCCTGT CCTGTGGGAT 
AAAAGCTGTC CCATGTATCA GAAGTTTTCA CCACTGGAAA GAGACCTGGA CGCCATGGAT 
CCTGCTACCT ATTCTTTAAC CATCATCTCT GGCCAGAATG TGTGTCCTAG TAACAGCACC 
GGAAGTCCGT GCATTGAAGT TGACGTCTTG GGCATGCCCC TGGACAGCTG CCATTTCCGC 
ACAAAACCCA TCCACCGAAA CACTCTGAAC CCCATGTGGA ATGAGCAGTT TCTCTTCCGG 
GTTCACTTTG AAGATCTTGT ATTTCTTCGT TTTGCAGTTG TGGAGAACAA CAGTTCGGCG 
ATAACGGCTC AGAGAATCAT TCCACTCAAA GCTTTAAAGA GAGGATATCG GCATCTTCAG 
CTGCGGAACC TCCACAACGA GATCTTGGAA ATCTCTAGCT TATTCATAAA CAGCAGGAGG 
ATGGAAGATA ATCCCTCTGG CAGTACCCGG CCAGCCTCTT TGATGTTCAA TACTGAAGAG 
AGGAAATGTT CTCAGACTCA CAAAGTCACA GTGCATGGTG TCCCAGGTCC AGAGCCCTTT 
GCTGTTTTCA CAATAAACGA AGGCACCAAG GCAAAGCAGC TTCTCCAACA GATTTTGGCA 
GTTGACCAGG ACACCAAACT TACAGCCGCA GACTATTTTC TGATGGAAGA GAAGCATTTT 
ATATCTAAGG AAAAGAATGA ATGCAGGAAA CAACCATTCC AGAGAGCTGT GGGCCCAGAG 
GAAGATATTG TGCAGATTTT AAACAGCTGG TTCCCAGAAG AAGGCTACGT AGGCAGGATT 
GTCTTAAAGC CCCAACAAGA AACCCTAGAA GAGAAAAACA TTGTCCACGA TGACAGAGAG 
GTGATCTTGA GCTCAGAAGA GGAGAGTTTC TTTGTCCAAG TGCACGATGT CTCTCCAGAG 
CAGCCTCGAA CTGTCATCAA AGCCCCACGT GTCAGCACTG CACAGGATGT GATTCAGCAG 
ACCTTGTGCA AAGCCAAATA TTCCTACAGC ATCCTGAACA ACCCCAATCC GTGTGACTAT 
GTGCTTTTGG AAGAGGTAAT GAAAGATGCA CCCAATAAGA AGTCTTCTAC CCCGAAGTCC 
TCACAGAGGA TCCTCTTGGA CCAAGAATGT GTGTTTCAAG CCCAAAGCAA GTGGAAGGGT 
GCAGGGAAAT TCATCCTTAA GCTAAAGGAG CAGGTGCAGG CATCCAGAGA AGATAAAAGG 
AGGGGCATCT CCTTTGCAAG TGAACTCAAG AAACTCACGA AGTCCACTAA GCAAACCCGA 
GGACTCACCT CGCCTCCTCA GCTTGTGGCC TCAGAAAGTG TGCAAAGCAA GGAGGAGAAA 
CCCATGGGTG CCTTGGCCTC TGGTGACACA GCGGGCTACC AGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plce1 ( XM_017588658.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444147.1 Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X3, mRNA.

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CloneID	ORa56295
Clone ID Related Accession (Same CDS sequence)	XM_017588656.1 , XM_017588657.1 , XM_017588658.1
Accession Version	XM_017588658.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCCG AAGAAATGGC CGCTTCCTTC CTCATCCCGG TGCCTCAAAG GAAAGTGGCT 
TCTGCCCAGT CAGTGGCAGA GGAGCGGGGT GAAAAGGTCT CAGAAGCCGG CATTCCTAAG 
ACACGAGCTG GCAGACAGGG TGGGCTCACC CCTCGTACCA TCTCACAGCG GAACGAACCT 
GAGGAAGAGT CTCCTAGAAC TGATTTTTCC CAGGTCTTCT CAATAGCGAG GGGGGAACTA 
GACAGCGACG AGAATCACAA TGAAAGGTGC TGGGAGGAAA ATGTGCCAGG TTCCACGAAA 
AACCACGCGG TTAACTGCAA CAGCTTATTG CAAAGCCACC AGCATGCGCT TCCCCCGAGC 
CAGCTCTGTG AAGTCTGTGA CTCTGTCACA GAAGAACACC TGTGTTTGCA GCCTGGGATT 
CCTTCTCCAC TGGAAAGGAA GGTGTTTCCT GGGATCGAAC TGGAGATGGA GGACTCTCCC 
ATGGACGTGA GCCCTTTGGG AAACCAGCCT GGGATCATGG AGTCCAGCGG ACCTCACTCT 
GACCGCAACA TGGCGGTATT TCATTTCCAT TATGCAGGTG ACAGGACAAT GCCAGGTGCT 
TTCCATACCC TGTCAGAAAA ATTCATTTTG GATGACTGTG CAAACTGTGT TACTCTTCCT 
GGGGGGCAGC AAAACAAAAA TTACATGGCG TATACTTGCA AACTGGTGGA GTTGACAAGA 
ACCTGTGGTA GTAAGAATGG GCAGCTGAAG TGTGACCACT GCACCTCGCT GCGGGATGAA 
TACCTGTGCT TTGAAAGCTC CTGCCGGAAG GCTGAGGCTC TCTCCTCAGG TGGTGGCTTT 
TGCGAGGACG GATTTACTCA TGGTCCCTCT GCCAAGACTT TTCTGAACCC TTTGGAGGAA 
TTCTCTGATA ATTGTGAAGA CGTGGACGAT ATTTTTAAAG GCAAAAAGGA GCGGTCCACT 
TTGTTAGTCC GAAGGTTTTG TAAAAATGAC AGGGAAGTTA AGAAGTCTGT GTATACTGGG 
ACAAGGGCCA TTGTGAGAAC CCTGCCTTCT GGCCACATTG GGCTGGCTGC TTGGAGTTAC 
GTCGATCAGA AGAAAGCTGG TCTCATGTGG CCTTGTGGGA ATGGAATGAG ACCTCTGTCC 
ACAGTGGACG TAAGGCAAAG TGGGAGACAG CGTCTGTCTG AAGCCCAGTG GTGTCTGATC 
TACAGTGCCG TGAGAAGAGA AGAGACAGAA GATACGGTTG GCTCTCTTCT GCATTGCTCC 
ACACAGCTCC CAACCCCTGA CACAGCCCAT GGAAGGATCG GAGATGGTCC CTGCCTAAAG 
CAATGTGTGC GAGACAGCGA GTGTGAGTAC CGTGCCACCC TCCAAAGGAC GTCCATAGCT 
CAGTACATCA CCGGTTCTCT CCTAGAGGCA ACCACATCTT TAGGAGCAAG AAGCAGCCTT 
CTCAGTTCCT TTGGAGGATC CACTGGGCGA ATAATGCTGA AAGAACGCCA ACCGGGCACT 
TCTATGGCCA ATTCCAGTCC TGTTCCTTCA AGTTCAGCTG GAATCAGCAA GGAGCTGATT 
GATCTCCAGC CCCTCATTCA GTTCCCGGAG GAAGTAGCCA GCATCTTAAC AGAACAGGAA 
CAGAACATTT ACCGCAGGGT CCTGCCCATG GACTACCTCT GTTTCTTAAC CCGGGACTTG 
AGTTCCCCCG AGTGTCAGAG ATCACTGCCC CGTCTCAAAG CATGCATCTC AGAATCAATC 
CTCATGTCTC AAAGTGGAGA GCACAATGCC CTTGAAGACC TCGTGATGAG ATTTAACGAG 
GTGAGCTCCT GGGTAACATG GCTGATCCTC ACGGCAGGCT CCATGGAGGA GAAGCGGGAA 
GTCTTTTCTT ATTTGGTGCA TGTGGCCAAA TGCTGCTGGA ACATGGGCAA CTACAATGCT 
GTCATGGAGT TCCTGGCTGG CCTCAGGTCA AGGAAAGTTC TGAAGATGTG GCAGTTCATG 
GACCAGTCTG ACATCGAGAC CATGAGGAGC CTGAAAGACG CCATGGCTCA GCATGAGTCT 
TCCGTGGAGT ACAAGAAGGT AGTGACCCGA GCCTTGCACA TCCCCGGCTG CAAAGTGGTT 
CCTTTCTGTG GGGTGTTCCT GAAGGAGCTC TGTGAGGTGC TTGATGGTGC CTCTGGCCTC 
CTGAAGCTCT GCCCACGATA CAGCTCCCAA GAAGAAGCCC TGGAGTTTGT AGCGGATTAC 
AGTGGACAAG ATAACTTCTT ACAGCGAGTG GGGCAGAATG GCTTGAAGAA TCCAGAGAAG 
GAGCTCACGG TCAACAGCAT CTTTCAGATC ATCCGGAGCT GCAGCCGAAG CTTGGAGACG 
GAGGACGAGG AGAGCGCCAG CGAAGGGAGC GGCTCTAGGA AAAACTCCCT CAAGGACAAA 
ACCCGGTGGC AGTTTATAAT TGGAGATTTG CTGGATTCAG ACAATGACAT CTTTGAGAAG 
TCCAAAGAGT GCGACCCTCA TGGATCAGAG GAGTCACAGA AAGCCTTTGA CCATGGGACA 
GAGCTCATCC CATGGTATGT GCTGTCCATC CAAGCGGATG TGCACCAGTT CCTCCTGCAG 
GGGGCCACAG TCATCCACTA TGACCAGGAC ACACATCTCT CCGCCCGCTG CTTTCTCCAG 
TTGCAGCCTG ACAATAGTAC CTTGACCTGG ATGAAACCTC CCACTGCCTC CCCAGCTGGT 
GCCAGACTCA AGCTCGGTGT GCTTAGTAAC GTGGCCGAAC CTGGCAAGTT CCCATCACTG 
GGCAACGCTG GGGTGAGCGG CCTGGTAGAG GGAATCCTGG ATCTGTTTTC AGTGAAGGCT 
GTGTACATGG GACACCCTGG CATTGATATA CACACTGTGT GTGTCCAGAA CAAACTTAGT 
AGCATGCTTC TGTCAGAGAC TGGGGTGACG CTGCTCTATG GGCTTCAGAC CACCGACAAT 
AGATTACTGC ACTTCGTGGC ACCAAAGCAC ACAGCTAAAA TGCTTTTCAG TGGATTGCTG 
GAACTCACTA CAGCTGTGAG AAAGATACGG AAGTTCCCTG ACCAAAGACA ACAGTGGCTT 
CGGAAACAGT ATGTCAGCTT CTATCAGGAG GATGGTCGGT ATGAAGGCCC AACCTTGGCA 
CATGCTGTGG AGTTGTTTGG TGGTAGGAGA TGGAGCACTA GAAACCCCAG CCCTGGGATG 
TCAGCAAAGA ATGCCGAGAA GCCCAACATG CAGAGAAACA ACACCCTAGG CATAAGCACC 
ACCAAGAAAA AGAAGAAAAT GCTCATGAGG GGGGAGAGTG GAGAGGTGAC GGACGATGAG 
ATGGCCACCC GCAAGGCCAA AATGTACAGA GAGTGTCGCA GCCGGAGCGG CTCTGATCCT 
CAAGAGGCTA ATGAACAGGA AGACTCAGAG GCCAATGTCA TCACTAACCC TCCCAACCCC 
CTCCATTCCA GAAGAGCCTA CTCATTGACC ACGGCTGGGT CCCCCAACTT GGCTACCGGG 
ATGTCATCTC CTATCAGTGC CTGGAGTAGC AGCAGCTGGC ACGGGCGGAT CAAAGGAGGA 
ATGAAAGGCT TCCAGAGCTT CATGGTCTCA GACAGTAACA TGAGTTTCAT TGAATTTGTC 
GAGCTGTTCA AATCATTCAG CATAAGGAGC CGCAAGGACT TGAAGGACAT CTTTGACATC 
TACTCTGTAC CCTGCAATCG GTCTGCTTCT GAGTCAGCCC CACTGTACAC CAACCTGACA 
ATCGAAGAAA ATACCAATGA CCTTCAGCCT GACCTAGATT TGCTGACTAG AAACGTCTCC 
GACTTGGGGC TGTTCATGAA GAGTAAGCAG CAGCTTTCTG ACAACCAGAG GCAGATATCT 
GACGCCATTG CTGCTGCAAG CATTGTGACT AATGGCACTG GGATTGAAAG CACGTCCCTG 
GGCATATTTG GGGTCGGCAT ACTCCAGCTC AACGACTTCC TGGTGAATTG CCAAGGAGAA 
CACTGCACAT ATGATGAAAT CCTCAGCATC ATCCAGAAGT TCGAGCCCAA CATCAGCATG 
TGTCATCAAG GTCTATTGTC CTTCGAAGGG TTTGCAAGGT TTCTGATGGA TAAAGACAAT 
TTCGCCTCAA AAAATGACGA GTCGCGGGAG AACAAGAAAG ACCTGCAGCT ACCCCTCTCC 
TACTATTACA TCGAATCTTC GCACAACACC TACCTCACCG GCCATCAGCT CAAGGGGGAG 
TCCTCTGTGG AGCTCTACAG CCAGGTCCTC TTGCAAGGAT GCAGAAGCAT AGAGCTGGAC 
TGCTGGGATG GAGATGATGG GATGCCCATT ATTTATCATG GACATACGCT GACAACCAAG 
ATCCCCTTCA AGGAAGTGGT GGAAGCCATC GATCGCAGTG CCTTCATCAC CTCCGACCTG 
CCAATCATCA TATCCATTGA GAACCATTGC TCATTGCCTC AGCAACGGAA AATGGCAGAA 
ATTTTCAAGA GTGTGTTTGG AGAAAAGCTG GTGGCTAAGT TCTTGTTCGA GACCGATTTC 
TCAGATGACC CAATGCTTCC CTCACCTGAC CAACTTAGAA GGAAAGTGCT TCTTAAAAAC 
AAGAAGCTAA AGGCCCATCA GACACCTGTC GACATCCTCA AACAAAAGGC TCATCAGTTA 
GCATCCATGC AAACACAGGC TTTCACTGGT GGGAATGCCA ACCCGCCACC TGCCAGTAAT 
GAGGAAGAGG AAGACGAGGA GGACGAATAT GATTATGATT ACGAGTCCCT GTCTGATGCA 
GATGTCCTTA CTGCTTCCCC TGTTCCTAAC AGTCAGGAAG ACAACATTCT GGAAGACAGA 
CCTGAGAACA AATCATGTGC CGACAAGCTT CAGTTTGAGT ACAATGAAGA AGTCCCCAAG 
AGGATAAAGA AAGCAGACAA CTCCTCCGGC AATAAGGGAA AGGTCTATGA CATGGAACTG 
GGAGAGGAGT TTTACCTTCC TCAGAACAAA AAAGAAAGCA GGCAGATTGC ACCGGAGCTC 
TCTGACCTCG TCATCTATTG CCAGGCGGTA AAATTTCCAG GCCTGTCAAC TCTAAATTCA 
TCTGGCTCTG GCAGAGGGAA AGAAAGGAAA AGCAGGAAGT CCATTTTTGG CAACAATCCC 
GGCAGAATGA GCCCAGGGGA GACAGCGTCA TTTAACAGAA CATCTGGAAA AAGTTCTTGT 
GAAGGAATTC GACAAATCTG GGAGGAACCT CCACTCAGCC CAAACACATC CCTCAGCGCC 
ATCATTAGAA CTCCTAAATG CTATCATATC TCGTCACTGA ATGAAAATGC CGCCAAACGT 
CTGTGTCGCA GGTATTCTCA GAAACTGATC CAGCACACTG CTTGCCAGCT GCTGAGGACC 
TACCCGGCAG CGACCAGAAT CGACTCATCC AATCCCAACC CCCTCATGTT CTGGCTCCAT 
GGGATACAGC TTGTAGCTCT AAACTACCAG ACGGATGACC TTCCTTTACA TTTAAACGCT 
GCCATGTTTG AGGCGAATGG TGGGTGTGGC TATGTTTTGA AGCCCCCTGT CCTGTGGGAT 
AAAAGCTGTC CCATGTATCA GAAGTTTTCA CCACTGGAAA GAGACCTGGA CGCCATGGAT 
CCTGCTACCT ATTCTTTAAC CATCATCTCT GGCCAGAATG TGTGTCCTAG TAACAGCACC 
GGAAGTCCGT GCATTGAAGT TGACGTCTTG GGCATGCCCC TGGACAGCTG CCATTTCCGC 
ACAAAACCCA TCCACCGAAA CACTCTGAAC CCCATGTGGA ATGAGCAGTT TCTCTTCCGG 
GTTCACTTTG AAGATCTTGT ATTTCTTCGT TTTGCAGTTG TGGAGAACAA CAGTTCGGCG 
ATAACGGCTC AGAGAATCAT TCCACTCAAA GCTTTAAAGA GAGGATATCG GCATCTTCAG 
CTGCGGAACC TCCACAACGA GATCTTGGAA ATCTCTAGCT TATTCATAAA CAGCAGGAGG 
ATGGAAGATA ATCCCTCTGG CAGTACCCGG CCAGCCTCTT TGATGTTCAA TACTGAAGAG 
AGGAAATGTT CTCAGACTCA CAAAGTCACA GTGCATGGTG TCCCAGGTCC AGAGCCCTTT 
GCTGTTTTCA CAATAAACGA AGGCACCAAG GCAAAGCAGC TTCTCCAACA GATTTTGGCA 
GTTGACCAGG ACACCAAACT TACAGCCGCA GACTATTTTC TGATGGAAGA GAAGCATTTT 
ATATCTAAGG AAAAGAATGA ATGCAGGAAA CAACCATTCC AGAGAGCTGT GGGCCCAGAG 
GAAGATATTG TGCAGATTTT AAACAGCTGG TTCCCAGAAG AAGGCTACGT AGGCAGGATT 
GTCTTAAAGC CCCAACAAGA AACCCTAGAA GAGAAAAACA TTGTCCACGA TGACAGAGAG 
GTGATCTTGA GCTCAGAAGA GGAGAGTTTC TTTGTCCAAG TGCACGATGT CTCTCCAGAG 
CAGCCTCGAA CTGTCATCAA AGCCCCACGT GTCAGCACTG CACAGGATGT GATTCAGCAG 
ACCTTGTGCA AAGCCAAATA TTCCTACAGC ATCCTGAACA ACCCCAATCC GTGTGACTAT 
GTGCTTTTGG AAGAGGTAAT GAAAGATGCA CCCAATAAGA AGTCTTCTAC CCCGAAGTCC 
TCACAGAGGA TCCTCTTGGA CCAAGAATGT GTGTTTCAAG CCCAAAGCAA GTGGAAGGGT 
GCAGGGAAAT TCATCCTTAA GCTAAAGGAG CAGGTGCAGG CATCCAGAGA AGATAAAAGG 
AGGGGCATCT CCTTTGCAAG TGAACTCAAG AAACTCACGA AGTCCACTAA GCAAACCCGA 
GGACTCACCT CGCCTCCTCA GCTTGTGGCC TCAGAAAGTG TGCAAAGCAA GGAGGAGAAA 
CCCATGGGTG CCTTGGCCTC TGGTGACACA GCGGGCTACC AGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444147.1
CDS	2143..9030
Translation	MTSEEMAASFLIPVPQRKVASAQSVAEERGEKVSEAGIPKTRAGRQGGLTPRTISQRNEPEEESPRTDFSQVFSIARGELDSDENHNERCWEENVPGSTKNHAVNCNSLLQSHQHALPPSQLCEVCDSVTEEHLCLQPGIPSPLERKVFPGIELEMEDSPMDVSPLGNQPGIMESSGPHSDRNMAVFHFHYAGDRTMPGAFHTLSEKFILDDCANCVTLPGGQQNKNYMAYTCKLVELTRTCGSKNGQLKCDHCTSLRDEYLCFESSCRKAEALSSGGGFCEDGFTHGPSAKTFLNPLEEFSDNCEDVDDIFKGKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLAAWSYVDQKKAGLMWPCGNGMRPLSTVDVRQSGRQRLSEAQWCLIYSAVRREETEDTVGSLLHCSTQLPTPDTAHGRIGDGPCLKQCVRDSECEYRATLQRTSIAQYITGSLLEATTSLGARSSLLSSFGGSTGRIMLKERQPGTSMANSSPVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRRVLPMDYLCFLTRDLSSPECQRSLPRLKACISESILMSQSGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSVEYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEEALEFVADYSGQDNFLQRVGQNGLKNPEKELTVNSIFQIIRSCSRSLETEDEESASEGSGSRKNSLKDKTRWQFIIGDLLDSDNDIFEKSKECDPHGSEESQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTASPAGARLKLGVLSNVAEPGKFPSLGNAGVSGLVEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSMLLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTTAVRKIRKFPDQRQQWLRKQYVSFYQEDGRYEGPTLAHAVELFGGRRWSTRNPSPGMSAKNAEKPNMQRNNTLGISTTKKKKKMLMRGESGEVTDDEMATRKAKMYRECRSRSGSDPQEANEQEDSEANVITNPPNPLHSRRAYSLTTAGSPNLATGMSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFIEFVELFKSFSIRSRKDLKDIFDIYSVPCNRSASESAPLYTNLTIEENTNDLQPDLDLLTRNVSDLGLFMKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPNISMCHQGLLSFEGFARFLMDKDNFASKNDESRENKKDLQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQLASMQTQAFTGGNANPPPASNEEEEDEEDEYDYDYESLSDADVLTASPVPNSQEDNILEDRPENKSCADKLQFEYNEEVPKRIKKADNSSGNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNSSGSGRGKERKSRKSIFGNNPGRMSPGETASFNRTSGKSSCEGIRQIWEEPPLSPNTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERDLDAMDPATYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLKALKRGYRHLQLRNLHNEILEISSLFINSRRMEDNPSGSTRPASLMFNTEERKCSQTHKVTVHGVPGPEPFAVFTINEGTKAKQLLQQILAVDQDTKLTAADYFLMEEKHFISKEKNECRKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKNIVHDDREVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPCDYVLLEEVMKDAPNKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRRGISFASELKKLTKSTKQTRGLTSPPQLVASESVQSKEEKPMGALASGDTAGYQS

Target ORF information:

RefSeq Version	XM_017588658.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588658.1

ORF Insert Sequence:

ATGACGTCCG AAGAAATGGC CGCTTCCTTC CTCATCCCGG TGCCTCAAAG GAAAGTGGCT 
TCTGCCCAGT CAGTGGCAGA GGAGCGGGGT GAAAAGGTCT CAGAAGCCGG CATTCCTAAG 
ACACGAGCTG GCAGACAGGG TGGGCTCACC CCTCGTACCA TCTCACAGCG GAACGAACCT 
GAGGAAGAGT CTCCTAGAAC TGATTTTTCC CAGGTCTTCT CAATAGCGAG GGGGGAACTA 
GACAGCGACG AGAATCACAA TGAAAGGTGC TGGGAGGAAA ATGTGCCAGG TTCCACGAAA 
AACCACGCGG TTAACTGCAA CAGCTTATTG CAAAGCCACC AGCATGCGCT TCCCCCGAGC 
CAGCTCTGTG AAGTCTGTGA CTCTGTCACA GAAGAACACC TGTGTTTGCA GCCTGGGATT 
CCTTCTCCAC TGGAAAGGAA GGTGTTTCCT GGGATCGAAC TGGAGATGGA GGACTCTCCC 
ATGGACGTGA GCCCTTTGGG AAACCAGCCT GGGATCATGG AGTCCAGCGG ACCTCACTCT 
GACCGCAACA TGGCGGTATT TCATTTCCAT TATGCAGGTG ACAGGACAAT GCCAGGTGCT 
TTCCATACCC TGTCAGAAAA ATTCATTTTG GATGACTGTG CAAACTGTGT TACTCTTCCT 
GGGGGGCAGC AAAACAAAAA TTACATGGCG TATACTTGCA AACTGGTGGA GTTGACAAGA 
ACCTGTGGTA GTAAGAATGG GCAGCTGAAG TGTGACCACT GCACCTCGCT GCGGGATGAA 
TACCTGTGCT TTGAAAGCTC CTGCCGGAAG GCTGAGGCTC TCTCCTCAGG TGGTGGCTTT 
TGCGAGGACG GATTTACTCA TGGTCCCTCT GCCAAGACTT TTCTGAACCC TTTGGAGGAA 
TTCTCTGATA ATTGTGAAGA CGTGGACGAT ATTTTTAAAG GCAAAAAGGA GCGGTCCACT 
TTGTTAGTCC GAAGGTTTTG TAAAAATGAC AGGGAAGTTA AGAAGTCTGT GTATACTGGG 
ACAAGGGCCA TTGTGAGAAC CCTGCCTTCT GGCCACATTG GGCTGGCTGC TTGGAGTTAC 
GTCGATCAGA AGAAAGCTGG TCTCATGTGG CCTTGTGGGA ATGGAATGAG ACCTCTGTCC 
ACAGTGGACG TAAGGCAAAG TGGGAGACAG CGTCTGTCTG AAGCCCAGTG GTGTCTGATC 
TACAGTGCCG TGAGAAGAGA AGAGACAGAA GATACGGTTG GCTCTCTTCT GCATTGCTCC 
ACACAGCTCC CAACCCCTGA CACAGCCCAT GGAAGGATCG GAGATGGTCC CTGCCTAAAG 
CAATGTGTGC GAGACAGCGA GTGTGAGTAC CGTGCCACCC TCCAAAGGAC GTCCATAGCT 
CAGTACATCA CCGGTTCTCT CCTAGAGGCA ACCACATCTT TAGGAGCAAG AAGCAGCCTT 
CTCAGTTCCT TTGGAGGATC CACTGGGCGA ATAATGCTGA AAGAACGCCA ACCGGGCACT 
TCTATGGCCA ATTCCAGTCC TGTTCCTTCA AGTTCAGCTG GAATCAGCAA GGAGCTGATT 
GATCTCCAGC CCCTCATTCA GTTCCCGGAG GAAGTAGCCA GCATCTTAAC AGAACAGGAA 
CAGAACATTT ACCGCAGGGT CCTGCCCATG GACTACCTCT GTTTCTTAAC CCGGGACTTG 
AGTTCCCCCG AGTGTCAGAG ATCACTGCCC CGTCTCAAAG CATGCATCTC AGAATCAATC 
CTCATGTCTC AAAGTGGAGA GCACAATGCC CTTGAAGACC TCGTGATGAG ATTTAACGAG 
GTGAGCTCCT GGGTAACATG GCTGATCCTC ACGGCAGGCT CCATGGAGGA GAAGCGGGAA 
GTCTTTTCTT ATTTGGTGCA TGTGGCCAAA TGCTGCTGGA ACATGGGCAA CTACAATGCT 
GTCATGGAGT TCCTGGCTGG CCTCAGGTCA AGGAAAGTTC TGAAGATGTG GCAGTTCATG 
GACCAGTCTG ACATCGAGAC CATGAGGAGC CTGAAAGACG CCATGGCTCA GCATGAGTCT 
TCCGTGGAGT ACAAGAAGGT AGTGACCCGA GCCTTGCACA TCCCCGGCTG CAAAGTGGTT 
CCTTTCTGTG GGGTGTTCCT GAAGGAGCTC TGTGAGGTGC TTGATGGTGC CTCTGGCCTC 
CTGAAGCTCT GCCCACGATA CAGCTCCCAA GAAGAAGCCC TGGAGTTTGT AGCGGATTAC 
AGTGGACAAG ATAACTTCTT ACAGCGAGTG GGGCAGAATG GCTTGAAGAA TCCAGAGAAG 
GAGCTCACGG TCAACAGCAT CTTTCAGATC ATCCGGAGCT GCAGCCGAAG CTTGGAGACG 
GAGGACGAGG AGAGCGCCAG CGAAGGGAGC GGCTCTAGGA AAAACTCCCT CAAGGACAAA 
ACCCGGTGGC AGTTTATAAT TGGAGATTTG CTGGATTCAG ACAATGACAT CTTTGAGAAG 
TCCAAAGAGT GCGACCCTCA TGGATCAGAG GAGTCACAGA AAGCCTTTGA CCATGGGACA 
GAGCTCATCC CATGGTATGT GCTGTCCATC CAAGCGGATG TGCACCAGTT CCTCCTGCAG 
GGGGCCACAG TCATCCACTA TGACCAGGAC ACACATCTCT CCGCCCGCTG CTTTCTCCAG 
TTGCAGCCTG ACAATAGTAC CTTGACCTGG ATGAAACCTC CCACTGCCTC CCCAGCTGGT 
GCCAGACTCA AGCTCGGTGT GCTTAGTAAC GTGGCCGAAC CTGGCAAGTT CCCATCACTG 
GGCAACGCTG GGGTGAGCGG CCTGGTAGAG GGAATCCTGG ATCTGTTTTC AGTGAAGGCT 
GTGTACATGG GACACCCTGG CATTGATATA CACACTGTGT GTGTCCAGAA CAAACTTAGT 
AGCATGCTTC TGTCAGAGAC TGGGGTGACG CTGCTCTATG GGCTTCAGAC CACCGACAAT 
AGATTACTGC ACTTCGTGGC ACCAAAGCAC ACAGCTAAAA TGCTTTTCAG TGGATTGCTG 
GAACTCACTA CAGCTGTGAG AAAGATACGG AAGTTCCCTG ACCAAAGACA ACAGTGGCTT 
CGGAAACAGT ATGTCAGCTT CTATCAGGAG GATGGTCGGT ATGAAGGCCC AACCTTGGCA 
CATGCTGTGG AGTTGTTTGG TGGTAGGAGA TGGAGCACTA GAAACCCCAG CCCTGGGATG 
TCAGCAAAGA ATGCCGAGAA GCCCAACATG CAGAGAAACA ACACCCTAGG CATAAGCACC 
ACCAAGAAAA AGAAGAAAAT GCTCATGAGG GGGGAGAGTG GAGAGGTGAC GGACGATGAG 
ATGGCCACCC GCAAGGCCAA AATGTACAGA GAGTGTCGCA GCCGGAGCGG CTCTGATCCT 
CAAGAGGCTA ATGAACAGGA AGACTCAGAG GCCAATGTCA TCACTAACCC TCCCAACCCC 
CTCCATTCCA GAAGAGCCTA CTCATTGACC ACGGCTGGGT CCCCCAACTT GGCTACCGGG 
ATGTCATCTC CTATCAGTGC CTGGAGTAGC AGCAGCTGGC ACGGGCGGAT CAAAGGAGGA 
ATGAAAGGCT TCCAGAGCTT CATGGTCTCA GACAGTAACA TGAGTTTCAT TGAATTTGTC 
GAGCTGTTCA AATCATTCAG CATAAGGAGC CGCAAGGACT TGAAGGACAT CTTTGACATC 
TACTCTGTAC CCTGCAATCG GTCTGCTTCT GAGTCAGCCC CACTGTACAC CAACCTGACA 
ATCGAAGAAA ATACCAATGA CCTTCAGCCT GACCTAGATT TGCTGACTAG AAACGTCTCC 
GACTTGGGGC TGTTCATGAA GAGTAAGCAG CAGCTTTCTG ACAACCAGAG GCAGATATCT 
GACGCCATTG CTGCTGCAAG CATTGTGACT AATGGCACTG GGATTGAAAG CACGTCCCTG 
GGCATATTTG GGGTCGGCAT ACTCCAGCTC AACGACTTCC TGGTGAATTG CCAAGGAGAA 
CACTGCACAT ATGATGAAAT CCTCAGCATC ATCCAGAAGT TCGAGCCCAA CATCAGCATG 
TGTCATCAAG GTCTATTGTC CTTCGAAGGG TTTGCAAGGT TTCTGATGGA TAAAGACAAT 
TTCGCCTCAA AAAATGACGA GTCGCGGGAG AACAAGAAAG ACCTGCAGCT ACCCCTCTCC 
TACTATTACA TCGAATCTTC GCACAACACC TACCTCACCG GCCATCAGCT CAAGGGGGAG 
TCCTCTGTGG AGCTCTACAG CCAGGTCCTC TTGCAAGGAT GCAGAAGCAT AGAGCTGGAC 
TGCTGGGATG GAGATGATGG GATGCCCATT ATTTATCATG GACATACGCT GACAACCAAG 
ATCCCCTTCA AGGAAGTGGT GGAAGCCATC GATCGCAGTG CCTTCATCAC CTCCGACCTG 
CCAATCATCA TATCCATTGA GAACCATTGC TCATTGCCTC AGCAACGGAA AATGGCAGAA 
ATTTTCAAGA GTGTGTTTGG AGAAAAGCTG GTGGCTAAGT TCTTGTTCGA GACCGATTTC 
TCAGATGACC CAATGCTTCC CTCACCTGAC CAACTTAGAA GGAAAGTGCT TCTTAAAAAC 
AAGAAGCTAA AGGCCCATCA GACACCTGTC GACATCCTCA AACAAAAGGC TCATCAGTTA 
GCATCCATGC AAACACAGGC TTTCACTGGT GGGAATGCCA ACCCGCCACC TGCCAGTAAT 
GAGGAAGAGG AAGACGAGGA GGACGAATAT GATTATGATT ACGAGTCCCT GTCTGATGCA 
GATGTCCTTA CTGCTTCCCC TGTTCCTAAC AGTCAGGAAG ACAACATTCT GGAAGACAGA 
CCTGAGAACA AATCATGTGC CGACAAGCTT CAGTTTGAGT ACAATGAAGA AGTCCCCAAG 
AGGATAAAGA AAGCAGACAA CTCCTCCGGC AATAAGGGAA AGGTCTATGA CATGGAACTG 
GGAGAGGAGT TTTACCTTCC TCAGAACAAA AAAGAAAGCA GGCAGATTGC ACCGGAGCTC 
TCTGACCTCG TCATCTATTG CCAGGCGGTA AAATTTCCAG GCCTGTCAAC TCTAAATTCA 
TCTGGCTCTG GCAGAGGGAA AGAAAGGAAA AGCAGGAAGT CCATTTTTGG CAACAATCCC 
GGCAGAATGA GCCCAGGGGA GACAGCGTCA TTTAACAGAA CATCTGGAAA AAGTTCTTGT 
GAAGGAATTC GACAAATCTG GGAGGAACCT CCACTCAGCC CAAACACATC CCTCAGCGCC 
ATCATTAGAA CTCCTAAATG CTATCATATC TCGTCACTGA ATGAAAATGC CGCCAAACGT 
CTGTGTCGCA GGTATTCTCA GAAACTGATC CAGCACACTG CTTGCCAGCT GCTGAGGACC 
TACCCGGCAG CGACCAGAAT CGACTCATCC AATCCCAACC CCCTCATGTT CTGGCTCCAT 
GGGATACAGC TTGTAGCTCT AAACTACCAG ACGGATGACC TTCCTTTACA TTTAAACGCT 
GCCATGTTTG AGGCGAATGG TGGGTGTGGC TATGTTTTGA AGCCCCCTGT CCTGTGGGAT 
AAAAGCTGTC CCATGTATCA GAAGTTTTCA CCACTGGAAA GAGACCTGGA CGCCATGGAT 
CCTGCTACCT ATTCTTTAAC CATCATCTCT GGCCAGAATG TGTGTCCTAG TAACAGCACC 
GGAAGTCCGT GCATTGAAGT TGACGTCTTG GGCATGCCCC TGGACAGCTG CCATTTCCGC 
ACAAAACCCA TCCACCGAAA CACTCTGAAC CCCATGTGGA ATGAGCAGTT TCTCTTCCGG 
GTTCACTTTG AAGATCTTGT ATTTCTTCGT TTTGCAGTTG TGGAGAACAA CAGTTCGGCG 
ATAACGGCTC AGAGAATCAT TCCACTCAAA GCTTTAAAGA GAGGATATCG GCATCTTCAG 
CTGCGGAACC TCCACAACGA GATCTTGGAA ATCTCTAGCT TATTCATAAA CAGCAGGAGG 
ATGGAAGATA ATCCCTCTGG CAGTACCCGG CCAGCCTCTT TGATGTTCAA TACTGAAGAG 
AGGAAATGTT CTCAGACTCA CAAAGTCACA GTGCATGGTG TCCCAGGTCC AGAGCCCTTT 
GCTGTTTTCA CAATAAACGA AGGCACCAAG GCAAAGCAGC TTCTCCAACA GATTTTGGCA 
GTTGACCAGG ACACCAAACT TACAGCCGCA GACTATTTTC TGATGGAAGA GAAGCATTTT 
ATATCTAAGG AAAAGAATGA ATGCAGGAAA CAACCATTCC AGAGAGCTGT GGGCCCAGAG 
GAAGATATTG TGCAGATTTT AAACAGCTGG TTCCCAGAAG AAGGCTACGT AGGCAGGATT 
GTCTTAAAGC CCCAACAAGA AACCCTAGAA GAGAAAAACA TTGTCCACGA TGACAGAGAG 
GTGATCTTGA GCTCAGAAGA GGAGAGTTTC TTTGTCCAAG TGCACGATGT CTCTCCAGAG 
CAGCCTCGAA CTGTCATCAA AGCCCCACGT GTCAGCACTG CACAGGATGT GATTCAGCAG 
ACCTTGTGCA AAGCCAAATA TTCCTACAGC ATCCTGAACA ACCCCAATCC GTGTGACTAT 
GTGCTTTTGG AAGAGGTAAT GAAAGATGCA CCCAATAAGA AGTCTTCTAC CCCGAAGTCC 
TCACAGAGGA TCCTCTTGGA CCAAGAATGT GTGTTTCAAG CCCAAAGCAA GTGGAAGGGT 
GCAGGGAAAT TCATCCTTAA GCTAAAGGAG CAGGTGCAGG CATCCAGAGA AGATAAAAGG 
AGGGGCATCT CCTTTGCAAG TGAACTCAAG AAACTCACGA AGTCCACTAA GCAAACCCGA 
GGACTCACCT CGCCTCCTCA GCTTGTGGCC TCAGAAAGTG TGCAAAGCAA GGAGGAGAAA 
CCCATGGGTG CCTTGGCCTC TGGTGACACA GCGGGCTACC AGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plce1 ( XM_017588659.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444148.1 Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X4, mRNA.

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CloneID	ORa56296
Clone ID Related Accession (Same CDS sequence)	XM_017588659.1
Accession Version	XM_017588659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCCG AAGAAATGGC CGCTTCCTTC CTCATCCCGG TGCCTCAAAG GAAAGTGGCT 
TCTGCCCAGT CAGTGGCAGA GGAGCGGGGT GAAAAGGTCT CAGAAGCCGG CATTCCTAAG 
ACACGAGCTG GCAGACAGGG TGGGCTCACC CCTCGTACCA TCTCACAGCG GAACGAACCT 
GAGGAAGAGT CTCCTAGAAC TGATTTTTCC CAGGTCTTCT CAATAGCGAG GGGGGAACTA 
GACAGCGACG AGAATCACAA TGAAAGGTGC TGGGAGGAAA ATGTGCCAGG TTCCACGAAA 
AACCACGCGG TTAACTGCAA CAGCTTATTG CAAAGCCACC AGCATGCGCT TCCCCCGAGC 
CAGCTCTGTG AAGTCTGTGA CTCTGTCACA GAAGAACACC TGTGTTTGCA GCCTGGGATT 
CCTTCTCCAC TGGAAAGGAA GGTGTTTCCT GGGATCGAAC TGGAGATGGA GGACTCTCCC 
ATGGACGTGA GCCCTTTGGG AAACCAGCCT GGGATCATGG AGTCCAGCGG ACCTCACTCT 
GACCGCAACA TGGCGGTATT TCATTTCCAT TATGCAGGTG ACAGGACAAT GCCAGGTGCT 
TTCCATACCC TGTCAGAAAA ATTCATTTTG GATGACTGTG CAAACTGTGT TACTCTTCCT 
GGGGGGCAGC AAAACAAAAA TTACATGGCG TATACTTGCA AACTGGTGGA GTTGACAAGA 
ACCTGTGGTA GTAAGAATGG GCAGCTGAAG TGTGACCACT GCACCTCGCT GCGGGATGAA 
TACCTGTGCT TTGAAAGCTC CTGCCGGAAG GCTGAGGCTC TCTCCTCAGG TGGTGGCTTT 
TGCGAGGACG GATTTACTCA TGGTCCCTCT GCCAAGACTT TTCTGAACCC TTTGGAGGAA 
TTCTCTGATA ATTGTGAAGA CGTGGACGAT ATTTTTAAAG GCAAAAAGGA GCGGTCCACT 
TTGTTAGTCC GAAGGTTTTG TAAAAATGAC AGGGAAGTTA AGAAGTCTGT GTATACTGGG 
ACAAGGGCCA TTGTGAGAAC CCTGCCTTCT GGCCACATTG GGCTGGCTGC TTGGAGTTAC 
GTCGATCAGA AGAAAGCTGG TCTCATGTGG CCTTGTGGGA ATGGAATGAG ACCTCTGTCC 
ACAGTGGACG TAAGGCAAAG TGGGAGACAG CGTCTGTCTG AAGCCCAGTG GTGTCTGATC 
TACAGTGCCG TGAGAAGAGA AGAGACAGAA GATACGGTTG GCTCTCTTCT GCATTGCTCC 
ACACAGCTCC CAACCCCTGA CACAGCCCAT GGAAGGATCG GAGATGGTCC CTGCCTAAAG 
CAATGTGTGC GAGACAGCGA GTGTGAGTAC CGTGCCACCC TCCAAAGGAC GTCCATAGCT 
CAGTACATCA CCGGTTCTCT CCTAGAGGCA ACCACATCTT TAGGAGCAAG AAGCAGCCTT 
CTCAGTTCCT TTGGAGGATC CACTGGGCGA ATAATGCTGA AAGAACGCCA ACCGGGCACT 
TCTATGGCCA ATTCCAGTCC TGTTCCTTCA AGTTCAGCTG GAATCAGCAA GGAGCTGATT 
GATCTCCAGC CCCTCATTCA GTTCCCGGAG GAAGTAGCCA GCATCTTAAC AGAACAGGAA 
CAGAACATTT ACCGCAGGGT CCTGCCCATG GACTACCTCT GTTTCTTAAC CCGGGACTTG 
AGTTCCCCCG AGTGTCAGAG ATCACTGCCC CGTCTCAAAG CATGCATCTC AGAATCAATC 
CTCATGTCTC AAAGTGGAGA GCACAATGCC CTTGAAGACC TCGTGATGAG ATTTAACGAG 
GTGAGCTCCT GGGTAACATG GCTGATCCTC ACGGCAGGCT CCATGGAGGA GAAGCGGGAA 
GTCTTTTCTT ATTTGGTGCA TGTGGCCAAA TGCTGCTGGA ACATGGGCAA CTACAATGCT 
GTCATGGAGT TCCTGGCTGG CCTCAGGTCA AGGAAAGTTC TGAAGATGTG GCAGTTCATG 
GACCAGTCTG ACATCGAGAC CATGAGGAGC CTGAAAGACG CCATGGCTCA GCATGAGTCT 
TCCGTGGAGT ACAAGAAGGT AGTGACCCGA GCCTTGCACA TCCCCGGCTG CAAAGTGGTT 
CCTTTCTGTG GGGTGTTCCT GAAGGAGCTC TGTGAGGTGC TTGATGGTGC CTCTGGCCTC 
CTGAAGCTCT GCCCACGATA CAGCTCCCAA GAAGAAGCCC TGGAGTTTGT AGCGGATTAC 
AGTGGACAAG ATAACTTCTT ACAGCGAGTG GGGCAGAATG GCTTGAAGAA TCCAGAGAAG 
GAGCTCACGG TCAACAGCAT CTTTCAGATC ATCCGGAGCT GCAGCCGAAG CTTGGAGACG 
GAGGACGAGG AGAGCGCCAG CGAAGGGAGC GGCTCTAGGA AAAACTCCCT CAAGGACAAA 
ACCCGGTGGC AGTTTATAAT TGGAGATTTG CTGGATTCAG ACAATGACAT CTTTGAGAAG 
TCCAAAGAGT GCGACCCTCA TGGATCAGAG GAGTCACAGA AAGCCTTTGA CCATGGGACA 
GAGCTCATCC CATGGTATGT GCTGTCCATC CAAGCGGATG TGCACCAGTT CCTCCTGCAG 
GGGGCCACAG TCATCCACTA TGACCAGGAC ACACATCTCT CCGCCCGCTG CTTTCTCCAG 
TTGCAGCCTG ACAATAGTAC CTTGACCTGG ATGAAACCTC CCACTGCCTC CCCAGCTGGT 
GCCAGACTCA AGCTCGGTGT GCTTAGTAAC GTGGCCGAAC CTGGCAAGTT CCCATCACTG 
GGCAACGCTG GGGTGAGCGG CCTGGTAGAG GGAATCCTGG ATCTGTTTTC AGTGAAGGCT 
GTGTACATGG GACACCCTGG CATTGATATA CACACTGTGT GTGTCCAGAA CAAACTTAGT 
AGCATGCTTC TGTCAGAGAC TGGGGTGACG CTGCTCTATG GGCTTCAGAC CACCGACAAT 
AGATTACTGC ACTTCGTGGC ACCAAAGCAC ACAGCTAAAA TGCTTTTCAG TGGATTGCTG 
GAACTCACTA CAGCTGTGAG AAAGATACGG AAGTTCCCTG ACCAAAGACA ACAGTGGCTT 
CGGAAACAGT ATGTCAGCTT CTATCAGGAG GATGGTCGGT ATGAAGGCCC AACCTTGGCA 
CATGCTGTGG AGTTGTTTGG TGGTAGGAGA TGGAGCACTA GAAACCCCAG CCCTGGGATG 
TCAGCAAAGA ATGCCGAGAA GCCCAACATG CAGAGAAACA ACACCCTAGG CATAAGCACC 
ACCAAGAAAA AGAAGAAAAT GCTCATGAGG GGGGAGAGTG GAGAGGTGAC GGACGATGAG 
ATGGCCACCC GCAAGGCCAA AATGTACAGA GAGTGTCGCA GCCGGAGCGG CTCTGATCCT 
CAAGAGGCTA ATGAACAGGA AGACTCAGAG GCCAATGTCA TCACTAACCC TCCCAACCCC 
CTCCATTCCA GAAGAGCCTA CTCATTGACC ACGGCTGGGT CCCCCAACTT GGCTACCGGG 
ATGTCATCTC CTATCAGTGC CTGGAGTAGC AGCAGCTGGC ACGGGCGGAT CAAAGGAGGA 
ATGAAAGGCT TCCAGAGCTT CATGGTCTCA GACAGTAACA TGAGTTTCAT TGAATTTGTC 
GAGCTGTTCA AATCATTCAG CATAAGGAGC CGCAAGGACT TGAAGGACAT CTTTGACATC 
TACTCTGTAC CCTGCAATCG GTCTGCTTCT GAGTCAGCCC CACTGTACAC CAACCTGACA 
ATCGAAGAAA ATACCAATGA CCTTCAGCCT GACCTAGATT TGCTGACTAG AAACGTCTCC 
GACTTGGGGC TGTTCATGAA GAGTAAGCAG CAGCTTTCTG ACAACCAGAG GCAGATATCT 
GACGCCATTG CTGCTGCAAG CATTGTGACT AATGGCACTG GGATTGAAAG CACGTCCCTG 
GGCATATTTG GGGTCGGCAT ACTCCAGCTC AACGACTTCC TGGTGAATTG CCAAGGAGAA 
CACTGCACAT ATGATGAAAT CCTCAGCATC ATCCAGAAGT TCGAGCCCAA CATCAGCATG 
TGTCATCAAG GTCTATTGTC CTTCGAAGGG TTTGCAAGGT TTCTGATGGA TAAAGACAAT 
TTCGCCTCAA AAAATGACGA GTCGCGGGAG AACAAGAAAG ACCTGCAGCT ACCCCTCTCC 
TACTATTACA TCGAATCTTC GCACAACACC TACCTCACCG GCCATCAGCT CAAGGGGGAG 
TCCTCTGTGG AGCTCTACAG CCAGGTCCTC TTGCAAGGAT GCAGAAGCAT AGAGCTGGAC 
TGCTGGGATG GAGATGATGG GATGCCCATT ATTTATCATG GACATACGCT GACAACCAAG 
ATCCCCTTCA AGGAAGTGGT GGAAGCCATC GATCGCAGTG CCTTCATCAC CTCCGACCTG 
CCAATCATCA TATCCATTGA GAACCATTGC TCATTGCCTC AGCAACGGAA AATGGCAGAA 
ATTTTCAAGA GTGTGTTTGG AGAAAAGCTG GTGGCTAAGT TCTTGTTCGA GACCGATTTC 
TCAGATGACC CAATGCTTCC CTCACCTGAC CAACTTAGAA GGAAAGTGCT TCTTAAAAAC 
AAGAAGCTAA AGGCCCATCA GACACCTGTC GACATCCTCA AACAAAAGGC TCATCAGTTA 
GCATCCATGC AAACACAGGC TTTCACTGGT GGGAATGCCA ACCCGCCACC TGCCAGTAAT 
GAGGAAGAGG AAGACGAGGA GGACGAATAT GATTATGATT ACGAGTCCCT GTCTGATGAT 
GTCCTTACTG CTTCCCCTGT TCCTAACAGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAGAACAAAT CATGTGCCGA CAAGCTTCAG TTTGAGTACA ATGAAGAAGT CCCCAAGAGG 
ATAAAGAAAG CAGACAACTC CTCCGGCAAT AAGGGAAAGG TCTATGACAT GGAACTGGGA 
GAGGAGTTTT ACCTTCCTCA GAACAAAAAA GAAAGCAGGC AGATTGCACC GGAGCTCTCT 
GACCTCGTCA TCTATTGCCA GGCGGTAAAA TTTCCAGGCC TGTCAACTCT AAATTCATCT 
GGCTCTGGCA GAGGGAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCCGGC 
AGAATGAGCC CAGGGGAGAC AGCGTCATTT AACAGAACAT CTGGAAAAAG TTCTTGTGAA 
GGAATTCGAC AAATCTGGGA GGAACCTCCA CTCAGCCCAA ACACATCCCT CAGCGCCATC 
ATTAGAACTC CTAAATGCTA TCATATCTCG TCACTGAATG AAAATGCCGC CAAACGTCTG 
TGTCGCAGGT ATTCTCAGAA ACTGATCCAG CACACTGCTT GCCAGCTGCT GAGGACCTAC 
CCGGCAGCGA CCAGAATCGA CTCATCCAAT CCCAACCCCC TCATGTTCTG GCTCCATGGG 
ATACAGCTTG TAGCTCTAAA CTACCAGACG GATGACCTTC CTTTACATTT AAACGCTGCC 
ATGTTTGAGG CGAATGGTGG GTGTGGCTAT GTTTTGAAGC CCCCTGTCCT GTGGGATAAA 
AGCTGTCCCA TGTATCAGAA GTTTTCACCA CTGGAAAGAG ACCTGGACGC CATGGATCCT 
GCTACCTATT CTTTAACCAT CATCTCTGGC CAGAATGTGT GTCCTAGTAA CAGCACCGGA 
AGTCCGTGCA TTGAAGTTGA CGTCTTGGGC ATGCCCCTGG ACAGCTGCCA TTTCCGCACA 
AAACCCATCC ACCGAAACAC TCTGAACCCC ATGTGGAATG AGCAGTTTCT CTTCCGGGTT 
CACTTTGAAG ATCTTGTATT TCTTCGTTTT GCAGTTGTGG AGAACAACAG TTCGGCGATA 
ACGGCTCAGA GAATCATTCC ACTCAAAGCT TTAAAGAGAG GATATCGGCA TCTTCAGCTG 
CGGAACCTCC ACAACGAGAT CTTGGAAATC TCTAGCTTAT TCATAAACAG CAGGAGGATG 
GAAGATAATC CCTCTGGCAG TACCCGGCCA GCCTCTTTGA TGTTCAATAC TGAAGAGAGG 
AAATGTTCTC AGACTCACAA AGTCACAGTG CATGGTGTCC CAGGTCCAGA GCCCTTTGCT 
GTTTTCACAA TAAACGAAGG CACCAAGGCA AAGCAGCTTC TCCAACAGAT TTTGGCAGTT 
GACCAGGACA CCAAACTTAC AGCCGCAGAC TATTTTCTGA TGGAAGAGAA GCATTTTATA 
TCTAAGGAAA AGAATGAATG CAGGAAACAA CCATTCCAGA GAGCTGTGGG CCCAGAGGAA 
GATATTGTGC AGATTTTAAA CAGCTGGTTC CCAGAAGAAG GCTACGTAGG CAGGATTGTC 
TTAAAGCCCC AACAAGAAAC CCTAGAAGAG AAAAACATTG TCCACGATGA CAGAGAGGTG 
ATCTTGAGCT CAGAAGAGGA GAGTTTCTTT GTCCAAGTGC ACGATGTCTC TCCAGAGCAG 
CCTCGAACTG TCATCAAAGC CCCACGTGTC AGCACTGCAC AGGATGTGAT TCAGCAGACC 
TTGTGCAAAG CCAAATATTC CTACAGCATC CTGAACAACC CCAATCCGTG TGACTATGTG 
CTTTTGGAAG AGGTAATGAA AGATGCACCC AATAAGAAGT CTTCTACCCC GAAGTCCTCA 
CAGAGGATCC TCTTGGACCA AGAATGTGTG TTTCAAGCCC AAAGCAAGTG GAAGGGTGCA 
GGGAAATTCA TCCTTAAGCT AAAGGAGCAG GTGCAGGCAT CCAGAGAAGA TAAAAGGAGG 
GGCATCTCCT TTGCAAGTGA ACTCAAGAAA CTCACGAAGT CCACTAAGCA AACCCGAGGA 
CTCACCTCGC CTCCTCAGCT TGTGGCCTCA GAAAGTGTGC AAAGCAAGGA GGAGAAACCC 
ATGGGTGCCT TGGCCTCTGG TGACACAGCG GGCTACCAGT CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444148.1
CDS	689..7573
Translation	MTSEEMAASFLIPVPQRKVASAQSVAEERGEKVSEAGIPKTRAGRQGGLTPRTISQRNEPEEESPRTDFSQVFSIARGELDSDENHNERCWEENVPGSTKNHAVNCNSLLQSHQHALPPSQLCEVCDSVTEEHLCLQPGIPSPLERKVFPGIELEMEDSPMDVSPLGNQPGIMESSGPHSDRNMAVFHFHYAGDRTMPGAFHTLSEKFILDDCANCVTLPGGQQNKNYMAYTCKLVELTRTCGSKNGQLKCDHCTSLRDEYLCFESSCRKAEALSSGGGFCEDGFTHGPSAKTFLNPLEEFSDNCEDVDDIFKGKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLAAWSYVDQKKAGLMWPCGNGMRPLSTVDVRQSGRQRLSEAQWCLIYSAVRREETEDTVGSLLHCSTQLPTPDTAHGRIGDGPCLKQCVRDSECEYRATLQRTSIAQYITGSLLEATTSLGARSSLLSSFGGSTGRIMLKERQPGTSMANSSPVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRRVLPMDYLCFLTRDLSSPECQRSLPRLKACISESILMSQSGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSVEYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEEALEFVADYSGQDNFLQRVGQNGLKNPEKELTVNSIFQIIRSCSRSLETEDEESASEGSGSRKNSLKDKTRWQFIIGDLLDSDNDIFEKSKECDPHGSEESQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTASPAGARLKLGVLSNVAEPGKFPSLGNAGVSGLVEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSMLLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTTAVRKIRKFPDQRQQWLRKQYVSFYQEDGRYEGPTLAHAVELFGGRRWSTRNPSPGMSAKNAEKPNMQRNNTLGISTTKKKKKMLMRGESGEVTDDEMATRKAKMYRECRSRSGSDPQEANEQEDSEANVITNPPNPLHSRRAYSLTTAGSPNLATGMSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFIEFVELFKSFSIRSRKDLKDIFDIYSVPCNRSASESAPLYTNLTIEENTNDLQPDLDLLTRNVSDLGLFMKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPNISMCHQGLLSFEGFARFLMDKDNFASKNDESRENKKDLQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQLASMQTQAFTGGNANPPPASNEEEEDEEDEYDYDYESLSDDVLTASPVPNSQEDNILEDRPENKSCADKLQFEYNEEVPKRIKKADNSSGNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNSSGSGRGKERKSRKSIFGNNPGRMSPGETASFNRTSGKSSCEGIRQIWEEPPLSPNTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERDLDAMDPATYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLKALKRGYRHLQLRNLHNEILEISSLFINSRRMEDNPSGSTRPASLMFNTEERKCSQTHKVTVHGVPGPEPFAVFTINEGTKAKQLLQQILAVDQDTKLTAADYFLMEEKHFISKEKNECRKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKNIVHDDREVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPCDYVLLEEVMKDAPNKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRRGISFASELKKLTKSTKQTRGLTSPPQLVASESVQSKEEKPMGALASGDTAGYQS

Target ORF information:

RefSeq Version	XM_017588659.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C, epsilon 1 (Plce1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588659.1

ORF Insert Sequence:

ATGACGTCCG AAGAAATGGC CGCTTCCTTC CTCATCCCGG TGCCTCAAAG GAAAGTGGCT 
TCTGCCCAGT CAGTGGCAGA GGAGCGGGGT GAAAAGGTCT CAGAAGCCGG CATTCCTAAG 
ACACGAGCTG GCAGACAGGG TGGGCTCACC CCTCGTACCA TCTCACAGCG GAACGAACCT 
GAGGAAGAGT CTCCTAGAAC TGATTTTTCC CAGGTCTTCT CAATAGCGAG GGGGGAACTA 
GACAGCGACG AGAATCACAA TGAAAGGTGC TGGGAGGAAA ATGTGCCAGG TTCCACGAAA 
AACCACGCGG TTAACTGCAA CAGCTTATTG CAAAGCCACC AGCATGCGCT TCCCCCGAGC 
CAGCTCTGTG AAGTCTGTGA CTCTGTCACA GAAGAACACC TGTGTTTGCA GCCTGGGATT 
CCTTCTCCAC TGGAAAGGAA GGTGTTTCCT GGGATCGAAC TGGAGATGGA GGACTCTCCC 
ATGGACGTGA GCCCTTTGGG AAACCAGCCT GGGATCATGG AGTCCAGCGG ACCTCACTCT 
GACCGCAACA TGGCGGTATT TCATTTCCAT TATGCAGGTG ACAGGACAAT GCCAGGTGCT 
TTCCATACCC TGTCAGAAAA ATTCATTTTG GATGACTGTG CAAACTGTGT TACTCTTCCT 
GGGGGGCAGC AAAACAAAAA TTACATGGCG TATACTTGCA AACTGGTGGA GTTGACAAGA 
ACCTGTGGTA GTAAGAATGG GCAGCTGAAG TGTGACCACT GCACCTCGCT GCGGGATGAA 
TACCTGTGCT TTGAAAGCTC CTGCCGGAAG GCTGAGGCTC TCTCCTCAGG TGGTGGCTTT 
TGCGAGGACG GATTTACTCA TGGTCCCTCT GCCAAGACTT TTCTGAACCC TTTGGAGGAA 
TTCTCTGATA ATTGTGAAGA CGTGGACGAT ATTTTTAAAG GCAAAAAGGA GCGGTCCACT 
TTGTTAGTCC GAAGGTTTTG TAAAAATGAC AGGGAAGTTA AGAAGTCTGT GTATACTGGG 
ACAAGGGCCA TTGTGAGAAC CCTGCCTTCT GGCCACATTG GGCTGGCTGC TTGGAGTTAC 
GTCGATCAGA AGAAAGCTGG TCTCATGTGG CCTTGTGGGA ATGGAATGAG ACCTCTGTCC 
ACAGTGGACG TAAGGCAAAG TGGGAGACAG CGTCTGTCTG AAGCCCAGTG GTGTCTGATC 
TACAGTGCCG TGAGAAGAGA AGAGACAGAA GATACGGTTG GCTCTCTTCT GCATTGCTCC 
ACACAGCTCC CAACCCCTGA CACAGCCCAT GGAAGGATCG GAGATGGTCC CTGCCTAAAG 
CAATGTGTGC GAGACAGCGA GTGTGAGTAC CGTGCCACCC TCCAAAGGAC GTCCATAGCT 
CAGTACATCA CCGGTTCTCT CCTAGAGGCA ACCACATCTT TAGGAGCAAG AAGCAGCCTT 
CTCAGTTCCT TTGGAGGATC CACTGGGCGA ATAATGCTGA AAGAACGCCA ACCGGGCACT 
TCTATGGCCA ATTCCAGTCC TGTTCCTTCA AGTTCAGCTG GAATCAGCAA GGAGCTGATT 
GATCTCCAGC CCCTCATTCA GTTCCCGGAG GAAGTAGCCA GCATCTTAAC AGAACAGGAA 
CAGAACATTT ACCGCAGGGT CCTGCCCATG GACTACCTCT GTTTCTTAAC CCGGGACTTG 
AGTTCCCCCG AGTGTCAGAG ATCACTGCCC CGTCTCAAAG CATGCATCTC AGAATCAATC 
CTCATGTCTC AAAGTGGAGA GCACAATGCC CTTGAAGACC TCGTGATGAG ATTTAACGAG 
GTGAGCTCCT GGGTAACATG GCTGATCCTC ACGGCAGGCT CCATGGAGGA GAAGCGGGAA 
GTCTTTTCTT ATTTGGTGCA TGTGGCCAAA TGCTGCTGGA ACATGGGCAA CTACAATGCT 
GTCATGGAGT TCCTGGCTGG CCTCAGGTCA AGGAAAGTTC TGAAGATGTG GCAGTTCATG 
GACCAGTCTG ACATCGAGAC CATGAGGAGC CTGAAAGACG CCATGGCTCA GCATGAGTCT 
TCCGTGGAGT ACAAGAAGGT AGTGACCCGA GCCTTGCACA TCCCCGGCTG CAAAGTGGTT 
CCTTTCTGTG GGGTGTTCCT GAAGGAGCTC TGTGAGGTGC TTGATGGTGC CTCTGGCCTC 
CTGAAGCTCT GCCCACGATA CAGCTCCCAA GAAGAAGCCC TGGAGTTTGT AGCGGATTAC 
AGTGGACAAG ATAACTTCTT ACAGCGAGTG GGGCAGAATG GCTTGAAGAA TCCAGAGAAG 
GAGCTCACGG TCAACAGCAT CTTTCAGATC ATCCGGAGCT GCAGCCGAAG CTTGGAGACG 
GAGGACGAGG AGAGCGCCAG CGAAGGGAGC GGCTCTAGGA AAAACTCCCT CAAGGACAAA 
ACCCGGTGGC AGTTTATAAT TGGAGATTTG CTGGATTCAG ACAATGACAT CTTTGAGAAG 
TCCAAAGAGT GCGACCCTCA TGGATCAGAG GAGTCACAGA AAGCCTTTGA CCATGGGACA 
GAGCTCATCC CATGGTATGT GCTGTCCATC CAAGCGGATG TGCACCAGTT CCTCCTGCAG 
GGGGCCACAG TCATCCACTA TGACCAGGAC ACACATCTCT CCGCCCGCTG CTTTCTCCAG 
TTGCAGCCTG ACAATAGTAC CTTGACCTGG ATGAAACCTC CCACTGCCTC CCCAGCTGGT 
GCCAGACTCA AGCTCGGTGT GCTTAGTAAC GTGGCCGAAC CTGGCAAGTT CCCATCACTG 
GGCAACGCTG GGGTGAGCGG CCTGGTAGAG GGAATCCTGG ATCTGTTTTC AGTGAAGGCT 
GTGTACATGG GACACCCTGG CATTGATATA CACACTGTGT GTGTCCAGAA CAAACTTAGT 
AGCATGCTTC TGTCAGAGAC TGGGGTGACG CTGCTCTATG GGCTTCAGAC CACCGACAAT 
AGATTACTGC ACTTCGTGGC ACCAAAGCAC ACAGCTAAAA TGCTTTTCAG TGGATTGCTG 
GAACTCACTA CAGCTGTGAG AAAGATACGG AAGTTCCCTG ACCAAAGACA ACAGTGGCTT 
CGGAAACAGT ATGTCAGCTT CTATCAGGAG GATGGTCGGT ATGAAGGCCC AACCTTGGCA 
CATGCTGTGG AGTTGTTTGG TGGTAGGAGA TGGAGCACTA GAAACCCCAG CCCTGGGATG 
TCAGCAAAGA ATGCCGAGAA GCCCAACATG CAGAGAAACA ACACCCTAGG CATAAGCACC 
ACCAAGAAAA AGAAGAAAAT GCTCATGAGG GGGGAGAGTG GAGAGGTGAC GGACGATGAG 
ATGGCCACCC GCAAGGCCAA AATGTACAGA GAGTGTCGCA GCCGGAGCGG CTCTGATCCT 
CAAGAGGCTA ATGAACAGGA AGACTCAGAG GCCAATGTCA TCACTAACCC TCCCAACCCC 
CTCCATTCCA GAAGAGCCTA CTCATTGACC ACGGCTGGGT CCCCCAACTT GGCTACCGGG 
ATGTCATCTC CTATCAGTGC CTGGAGTAGC AGCAGCTGGC ACGGGCGGAT CAAAGGAGGA 
ATGAAAGGCT TCCAGAGCTT CATGGTCTCA GACAGTAACA TGAGTTTCAT TGAATTTGTC 
GAGCTGTTCA AATCATTCAG CATAAGGAGC CGCAAGGACT TGAAGGACAT CTTTGACATC 
TACTCTGTAC CCTGCAATCG GTCTGCTTCT GAGTCAGCCC CACTGTACAC CAACCTGACA 
ATCGAAGAAA ATACCAATGA CCTTCAGCCT GACCTAGATT TGCTGACTAG AAACGTCTCC 
GACTTGGGGC TGTTCATGAA GAGTAAGCAG CAGCTTTCTG ACAACCAGAG GCAGATATCT 
GACGCCATTG CTGCTGCAAG CATTGTGACT AATGGCACTG GGATTGAAAG CACGTCCCTG 
GGCATATTTG GGGTCGGCAT ACTCCAGCTC AACGACTTCC TGGTGAATTG CCAAGGAGAA 
CACTGCACAT ATGATGAAAT CCTCAGCATC ATCCAGAAGT TCGAGCCCAA CATCAGCATG 
TGTCATCAAG GTCTATTGTC CTTCGAAGGG TTTGCAAGGT TTCTGATGGA TAAAGACAAT 
TTCGCCTCAA AAAATGACGA GTCGCGGGAG AACAAGAAAG ACCTGCAGCT ACCCCTCTCC 
TACTATTACA TCGAATCTTC GCACAACACC TACCTCACCG GCCATCAGCT CAAGGGGGAG 
TCCTCTGTGG AGCTCTACAG CCAGGTCCTC TTGCAAGGAT GCAGAAGCAT AGAGCTGGAC 
TGCTGGGATG GAGATGATGG GATGCCCATT ATTTATCATG GACATACGCT GACAACCAAG 
ATCCCCTTCA AGGAAGTGGT GGAAGCCATC GATCGCAGTG CCTTCATCAC CTCCGACCTG 
CCAATCATCA TATCCATTGA GAACCATTGC TCATTGCCTC AGCAACGGAA AATGGCAGAA 
ATTTTCAAGA GTGTGTTTGG AGAAAAGCTG GTGGCTAAGT TCTTGTTCGA GACCGATTTC 
TCAGATGACC CAATGCTTCC CTCACCTGAC CAACTTAGAA GGAAAGTGCT TCTTAAAAAC 
AAGAAGCTAA AGGCCCATCA GACACCTGTC GACATCCTCA AACAAAAGGC TCATCAGTTA 
GCATCCATGC AAACACAGGC TTTCACTGGT GGGAATGCCA ACCCGCCACC TGCCAGTAAT 
GAGGAAGAGG AAGACGAGGA GGACGAATAT GATTATGATT ACGAGTCCCT GTCTGATGAT 
GTCCTTACTG CTTCCCCTGT TCCTAACAGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAGAACAAAT CATGTGCCGA CAAGCTTCAG TTTGAGTACA ATGAAGAAGT CCCCAAGAGG 
ATAAAGAAAG CAGACAACTC CTCCGGCAAT AAGGGAAAGG TCTATGACAT GGAACTGGGA 
GAGGAGTTTT ACCTTCCTCA GAACAAAAAA GAAAGCAGGC AGATTGCACC GGAGCTCTCT 
GACCTCGTCA TCTATTGCCA GGCGGTAAAA TTTCCAGGCC TGTCAACTCT AAATTCATCT 
GGCTCTGGCA GAGGGAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCCGGC 
AGAATGAGCC CAGGGGAGAC AGCGTCATTT AACAGAACAT CTGGAAAAAG TTCTTGTGAA 
GGAATTCGAC AAATCTGGGA GGAACCTCCA CTCAGCCCAA ACACATCCCT CAGCGCCATC 
ATTAGAACTC CTAAATGCTA TCATATCTCG TCACTGAATG AAAATGCCGC CAAACGTCTG 
TGTCGCAGGT ATTCTCAGAA ACTGATCCAG CACACTGCTT GCCAGCTGCT GAGGACCTAC 
CCGGCAGCGA CCAGAATCGA CTCATCCAAT CCCAACCCCC TCATGTTCTG GCTCCATGGG 
ATACAGCTTG TAGCTCTAAA CTACCAGACG GATGACCTTC CTTTACATTT AAACGCTGCC 
ATGTTTGAGG CGAATGGTGG GTGTGGCTAT GTTTTGAAGC CCCCTGTCCT GTGGGATAAA 
AGCTGTCCCA TGTATCAGAA GTTTTCACCA CTGGAAAGAG ACCTGGACGC CATGGATCCT 
GCTACCTATT CTTTAACCAT CATCTCTGGC CAGAATGTGT GTCCTAGTAA CAGCACCGGA 
AGTCCGTGCA TTGAAGTTGA CGTCTTGGGC ATGCCCCTGG ACAGCTGCCA TTTCCGCACA 
AAACCCATCC ACCGAAACAC TCTGAACCCC ATGTGGAATG AGCAGTTTCT CTTCCGGGTT 
CACTTTGAAG ATCTTGTATT TCTTCGTTTT GCAGTTGTGG AGAACAACAG TTCGGCGATA 
ACGGCTCAGA GAATCATTCC ACTCAAAGCT TTAAAGAGAG GATATCGGCA TCTTCAGCTG 
CGGAACCTCC ACAACGAGAT CTTGGAAATC TCTAGCTTAT TCATAAACAG CAGGAGGATG 
GAAGATAATC CCTCTGGCAG TACCCGGCCA GCCTCTTTGA TGTTCAATAC TGAAGAGAGG 
AAATGTTCTC AGACTCACAA AGTCACAGTG CATGGTGTCC CAGGTCCAGA GCCCTTTGCT 
GTTTTCACAA TAAACGAAGG CACCAAGGCA AAGCAGCTTC TCCAACAGAT TTTGGCAGTT 
GACCAGGACA CCAAACTTAC AGCCGCAGAC TATTTTCTGA TGGAAGAGAA GCATTTTATA 
TCTAAGGAAA AGAATGAATG CAGGAAACAA CCATTCCAGA GAGCTGTGGG CCCAGAGGAA 
GATATTGTGC AGATTTTAAA CAGCTGGTTC CCAGAAGAAG GCTACGTAGG CAGGATTGTC 
TTAAAGCCCC AACAAGAAAC CCTAGAAGAG AAAAACATTG TCCACGATGA CAGAGAGGTG 
ATCTTGAGCT CAGAAGAGGA GAGTTTCTTT GTCCAAGTGC ACGATGTCTC TCCAGAGCAG 
CCTCGAACTG TCATCAAAGC CCCACGTGTC AGCACTGCAC AGGATGTGAT TCAGCAGACC 
TTGTGCAAAG CCAAATATTC CTACAGCATC CTGAACAACC CCAATCCGTG TGACTATGTG 
CTTTTGGAAG AGGTAATGAA AGATGCACCC AATAAGAAGT CTTCTACCCC GAAGTCCTCA 
CAGAGGATCC TCTTGGACCA AGAATGTGTG TTTCAAGCCC AAAGCAAGTG GAAGGGTGCA 
GGGAAATTCA TCCTTAAGCT AAAGGAGCAG GTGCAGGCAT CCAGAGAAGA TAAAAGGAGG 
GGCATCTCCT TTGCAAGTGA ACTCAAGAAA CTCACGAAGT CCACTAAGCA AACCCGAGGA 
CTCACCTCGC CTCCTCAGCT TGTGGCCTCA GAAAGTGTGC AAAGCAAGGA GGAGAAACCC 
ATGGGTGCCT TGGCCTCTGG TGACACAGCG GGCTACCAGT CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Compartmentalized cyclic nucleotides have opposing effects on regulation of hypertrophic phospholipase Cε signaling in cardiac myocytes.
Journal of molecular and cellular cardiology12151-59(2018 08)
Nash CA,Brown LM,Malik S,Cheng X,Smrcka AV

G protein βγ subunits directly interact with and activate phospholipase Cϵ.
The Journal of biological chemistry293(17)6387-6397(2018 04)
Madukwe JC,Garland-Kuntz EE,Lyon AM,Smrcka AV

Phospholipase Cε Modulates Rap1 Activity and the Endothelial Barrier.
PloS one11(9)e0162338(2016)
DiStefano PV,Smrcka AV,Glading AJ

Phospholipase C epsilon scaffolds to muscle-specific A kinase anchoring protein (mAKAPbeta) and integrates multiple hypertrophic stimuli in cardiac myocytes.
The Journal of biological chemistry286(26)23012-21(2011 Jul)
Zhang L,Malik S,Kelley GG,Kapiloff MS,Smrcka AV

Mutational analysis of the PLCE1 gene in steroid resistant nephrotic syndrome.
Journal of medical genetics47(7)445-52(2010 Jul)
Boyer O,Benoit G,Gribouval O,Nevo F,Pawtowski A,Bilge I,Bircan Z,Deschênes G,Guay-Woodford LM,Hall M,Macher MA,Soulami K,Stefanidis CJ,Weiss R,Loirat C,Gubler MC,Antignac C

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Plce1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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