Tdg cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tdg
Entrez Gene ID	114521
Full Name	thymine-DNA glycosylase
General protein information	Preferred Names thymine-DNA glycosylase Names G/T mismatch-specific thymine DNA glycosylase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q13
Summary	a T:G mismatch-specific thymine DNA glycosylase; acts as a transcriptional repressor of TTF-1 transcriptional activity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053729.1	NP_446181.1	G/T mismatch-specific thymine DNA glycosylase

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	TDG	NP_990081.1
Xenopus tropicalis (tropical clawed frog)	tdg	NP_001004982.1
Homo sapiens (human)	TDG	NP_003202.3
Canis lupus familiaris (dog)	TDG	XP_005629358.1
Mus musculus (house mouse)	Gm5806	XP_006521693.1
Bos taurus (cattle)	TDG	NP_001077165.1
Macaca mulatta (Rhesus monkey)	TDG	XP_001096295.1
Rattus norvegicus (Norway rat)	Tdg	NP_446181.1
Danio rerio (zebrafish)	tdg	NP_001018587.1
Pan troglodytes (chimpanzee)	TDG	XP_509324.3
Mus musculus (house mouse)	Tdg	NP_766140.2

Gene Ontology
Bibliography

Related articles in PubMed

Lesion processing by a repair enzyme is severely curtailed by residues needed to prevent aberrant activity on undamaged DNA.
Maiti A, Noon MS, MacKerell AD Jr, Pozharski E, Drohat AC
Proceedings of the National Academy of Sciences of the United States of America109(21)8091-6(2012 May)

Thymine DNA glycosylase specifically recognizes 5-carboxylcytosine-modified DNA.
Zhang L, Lu X, Lu J, Liang H, Dai Q, Xu GL, Luo C, Jiang H, He C
Nature chemical biology8(4)328-30(2012 Feb)

Thymine DNA glycosylase can rapidly excise 5-formylcytosine and 5-carboxylcytosine: potential implications for active demethylation of CpG sites.
Maiti A, Drohat AC
The Journal of biological chemistry286(41)35334-8(2011 Oct)

Tet-mediated formation of 5-carboxylcytosine and its excision by TDG in mammalian DNA.
He YF, Li BZ, Li Z, Liu P, Wang Y, Tang Q, Ding J, Jia Y, Chen Z, Li L, Sun Y, Li X, Dai Q, Song CX, Zhang K, He C, Xu GL
Science (New York, N.Y.)333(6047)1303-7(2011 Sep)

Thymine DNA glycosylase is essential for active DNA demethylation by linked deamination-base excision repair.
Cortellino S, Xu J, Sannai M, Moore R, Caretti E, Cigliano A, Le Coz M, Devarajan K, Wessels A, Soprano D, Abramowitz LK, Bartolomei MS, Rambow F, Bassi MR, Bruno T, Fanciulli M, Renner C, Klein-Szanto AJ, Matsumoto Y, Kobi D, Davidson I, Alberti C, Larue L, Bellacosa A
Cell146(1)67-79(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tdg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tdg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10446	NM_053729.1 Latest version!	Rattus norvegicus thymine-DNA glycosylase (Tdg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tdg ( NM_053729.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446181.1 Rattus norvegicus thymine-DNA glycosylase (Tdg), mRNA.

Price & Availability↑

CloneID	ORa10446
Clone ID Related Accession (Same CDS sequence)	NM_053729.1
Accession Version	NM_053729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527264000000
Organism	Rattus norvegicus(Norway rat)
Product	G/T mismatch-specific thymine DNA glycosylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY026945.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY026945.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGATGGCGG AGGCTCCGAA CATGGCAGAC GTGGCCGGAC AGCAGATGCC TGCAGAAGCT 
CCTGCCCAGG ATCCTGTGCC AGAGGCTCCA AAGAGAAGGA AAAGGAAAAC CAGGGCAGCA 
GAGGCCCAGG ACCCAGTGGA GCCCAAAAAG CCTGCTGCCT CAAAGAAATC TGGCAAGTCC 
ACAAAATCAA AAGAAAAGCA AGAGAAAATC ACAGACACCT TTAAAGTGAA AAGGAAAGTG 
GACCGGTTCA ACGGTGTCTC TGAAGCTGAG CTTCTGACCA AGACTCTCCC TGACATTTTG 
ACCTTCAATC TGGATATTGT AATTATTGGC ATCAACCCAG GATTAATGGC CGCTTACAAA 
GGGCATCATT ACCCTGGGCC CGGAAATCAC TTCTGGAAAT GTCTGTTCAT GTCTGGGTTG 
AGCGAGGTGC AGCTGAGTCA CATGGATGAC CACACCTTAC CTGGAAAATA CGGCATCGGA 
TTCACCAACA TGGTGGAACG GACAACGCCA GGCAGCAAGG ATCTGTCTAG TAAAGAATTC 
CGGGAAGGAG GGCGTATCCT AGTGCAGAAA CTACAGAAAT ATCAGCCACG AATAGCGGTG 
TTTAATGGAA AATGTATTTA TGAGATTTTC AGTAAAGAAG TTTTTGGAGT AAAGGTTAAG 
AACTTGGAAT TTGGGCTTCA ACCGCACAAG ATCCCAGACA CAGAAACTCT CTGCTACGTC 
ATGCCGTCCT CCAGTGCCCG GTGCGCTCAG TTCCCTCGGG CGCAGGACAA AGTCCATTAC 
TACATTAAGC TGAAGGACTT GCGAGACCAG CTGAAGGGCA TCGAGCGCAG CACGGACGTT 
CAGGAGGTGC AGTACACGTT CGACCTGCAG CTCGCACAAG AGGACGCAAA GAGGACGGCT 
GTTAAGGAAG AAAAGTATGA CCCAGGCTAT GAGGCAGCGT ATGGCGGTGC CTGTGGTGAA 
AACCCGTGTA ATGGGGAACC TTGTGGCTTT GCTTCAAATG GGCTAACAGC CAACAGTGCG 
GAGCTGGGAG GAGAATCAGC TCCGAGCGAC GTTCCAAACG GGCAGTGGAT GGCGCAGTCG 
TTTGCAGAGC AGATCCCTTC TTTCAATAAT TGTGGCACAG GAGAGCAGGA AGCAGGAAGC 
CATGCAGGAA GCCATGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446181.1
CDS	125..1285
Translation	MMAEAPNMADVAGQQMPAEAPAQDPVPEAPKRRKRKTRAAEAQDPVEPKKPAASKKSGKSTKSKEKQEKITDTFKVKRKVDRFNGVSEAELLTKTLPDILTFNLDIVIIGINPGLMAAYKGHHYPGPGNHFWKCLFMSGLSEVQLSHMDDHTLPGKYGIGFTNMVERTTPGSKDLSSKEFREGGRILVQKLQKYQPRIAVFNGKCIYEIFSKEVFGVKVKNLEFGLQPHKIPDTETLCYVMPSSSARCAQFPRAQDKVHYYIKLKDLRDQLKGIERSTDVQEVQYTFDLQLAQEDAKRTAVKEEKYDPGYEAAYGGACGENPCNGEPCGFASNGLTANSAELGGESAPSDVPNGQWMAQSFAEQIPSFNNCGTGEQEAGSHAGSHA

Target ORF information:

RefSeq Version	NM_053729.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus thymine-DNA glycosylase (Tdg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053729.1

ORF Insert Sequence:

ATGATGGCGG AGGCTCCGAA CATGGCAGAC GTGGCCGGAC AGCAGATGCC TGCAGAAGCT 
CCTGCCCAGG ATCCTGTGCC AGAGGCTCCA AAGAGAAGGA AAAGGAAAAC CAGGGCAGCA 
GAGGCCCAGG ACCCAGTGGA GCCCAAAAAG CCTGCTGCCT CAAAGAAATC TGGCAAGTCC 
ACAAAATCAA AAGAAAAGCA AGAGAAAATC ACAGACACCT TTAAAGTGAA AAGGAAAGTG 
GACCGGTTCA ACGGTGTCTC TGAAGCTGAG CTTCTGACCA AGACTCTCCC TGACATTTTG 
ACCTTCAATC TGGATATTGT AATTATTGGC ATCAACCCAG GATTAATGGC CGCTTACAAA 
GGGCATCATT ACCCTGGGCC CGGAAATCAC TTCTGGAAAT GTCTGTTCAT GTCTGGGTTG 
AGCGAGGTGC AGCTGAGTCA CATGGATGAC CACACCTTAC CTGGAAAATA CGGCATCGGA 
TTCACCAACA TGGTGGAACG GACAACGCCA GGCAGCAAGG ATCTGTCTAG TAAAGAATTC 
CGGGAAGGAG GGCGTATCCT AGTGCAGAAA CTACAGAAAT ATCAGCCACG AATAGCGGTG 
TTTAATGGAA AATGTATTTA TGAGATTTTC AGTAAAGAAG TTTTTGGAGT AAAGGTTAAG 
AACTTGGAAT TTGGGCTTCA ACCGCACAAG ATCCCAGACA CAGAAACTCT CTGCTACGTC 
ATGCCGTCCT CCAGTGCCCG GTGCGCTCAG TTCCCTCGGG CGCAGGACAA AGTCCATTAC 
TACATTAAGC TGAAGGACTT GCGAGACCAG CTGAAGGGCA TCGAGCGCAG CACGGACGTT 
CAGGAGGTGC AGTACACGTT CGACCTGCAG CTCGCACAAG AGGACGCAAA GAGGACGGCT 
GTTAAGGAAG AAAAGTATGA CCCAGGCTAT GAGGCAGCGT ATGGCGGTGC CTGTGGTGAA 
AACCCGTGTA ATGGGGAACC TTGTGGCTTT GCTTCAAATG GGCTAACAGC CAACAGTGCG 
GAGCTGGGAG GAGAATCAGC TCCGAGCGAC GTTCCAAACG GGCAGTGGAT GGCGCAGTCG 
TTTGCAGAGC AGATCCCTTC TTTCAATAAT TGTGGCACAG GAGAGCAGGA AGCAGGAAGC 
CATGCAGGAA GCCATGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lesion processing by a repair enzyme is severely curtailed by residues needed to prevent aberrant activity on undamaged DNA.
Proceedings of the National Academy of Sciences of the United States of America109(21)8091-6(2012 May)
Maiti A,Noon MS,MacKerell AD Jr,Pozharski E,Drohat AC

Thymine DNA glycosylase specifically recognizes 5-carboxylcytosine-modified DNA.
Nature chemical biology8(4)328-30(2012 Feb)
Zhang L,Lu X,Lu J,Liang H,Dai Q,Xu GL,Luo C,Jiang H,He C

Thymine DNA glycosylase can rapidly excise 5-formylcytosine and 5-carboxylcytosine: potential implications for active demethylation of CpG sites.
The Journal of biological chemistry286(41)35334-8(2011 Oct)
Maiti A,Drohat AC

Tet-mediated formation of 5-carboxylcytosine and its excision by TDG in mammalian DNA.
Science (New York, N.Y.)333(6047)1303-7(2011 Sep)
He YF,Li BZ,Li Z,Liu P,Wang Y,Tang Q,Ding J,Jia Y,Chen Z,Li L,Sun Y,Li X,Dai Q,Song CX,Zhang K,He C,Xu GL

Thymine DNA glycosylase is essential for active DNA demethylation by linked deamination-base excision repair.
Cell146(1)67-79(2011 Jul)
Cortellino S,Xu J,Sannai M,Moore R,Caretti E,Cigliano A,Le Coz M,Devarajan K,Wessels A,Soprano D,Abramowitz LK,Bartolomei MS,Rambow F,Bassi MR,Bruno T,Fanciulli M,Renner C,Klein-Szanto AJ,Matsumoto Y,Kobi D,Davidson I,Alberti C,Larue L,Bellacosa A

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Tdg cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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