Dag1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dag1
Entrez Gene ID	114489
Full Name	dystroglycan 1
General protein information	Preferred Names dystroglycan 1 Names dystroglycan alpha-dystroglycan beta-dystroglycan dystroglycan 1 (dystrophin-associated glycoprotein 1)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	laminin-binding glycoprotein involved in linking the extracellular matrix to the actin cytoskeleton [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053697.1	NP_446149.1	dystroglycan precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DAG1	NP_776587.1
Danio rerio (zebrafish)	dag1	NP_775381.1
Canis lupus familiaris (dog)	DAG1	NP_001029164.1
Homo sapiens (human)	DAG1	NP_001171105.1
Mus musculus (house mouse)	Dag1	NP_001263421.1
Rattus norvegicus (Norway rat)	Dag1	NP_446149.1
Pan troglodytes (chimpanzee)	DAG1	XP_003339091.1
Macaca mulatta (Rhesus monkey)	DAG1	XP_001108436.1
Xenopus tropicalis (tropical clawed frog)	dag1	NP_001016518.1
Gallus gallus (chicken)	DAG1	NP_001091009.1

Gene Ontology
Bibliography

Related articles in PubMed

Neuronal Dystroglycan Is Necessary for Formation and Maintenance of Functional CCK-Positive Basket Cell Terminals on Pyramidal Cells.
Früh S, Romanos J, Panzanelli P, Bürgisser D, Tyagarajan SK, Campbell KP, Santello M, Fritschy JM
The Journal of neuroscience : the official journal of the Society for Neuroscience36(40)10296-10313(2016 10)

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Loss of AQP4 polarized localization with loss of β-dystroglycan immunoreactivity may induce brain edema following intracerebral hemorrhage.
Qiu GP, Xu J, Zhuo F, Sun SQ, Liu H, Yang M, Huang J, Lu WT, Huang SQ
Neuroscience letters58842-8(2015 Feb)

Lost Polarization of Aquaporin4 and Dystroglycan in the Core Lesion after Traumatic Brain Injury Suggests Functional Divergence in Evolution.
Liu H, Qiu Gp, Zhuo F, Yu Wh, Sun Sq, Li Fh, Yang M
BioMed research international2015471631(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dag1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dag1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10339	NM_053697.1 Latest version!	Rattus norvegicus dystroglycan 1 (Dag1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dag1 ( NM_053697.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446149.1 Rattus norvegicus dystroglycan 1 (Dag1), mRNA.

Price & Availability↑

CloneID	ORa10339
Clone ID Related Accession (Same CDS sequence)	NM_053697.1
Accession Version	NM_053697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	dystroglycan precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07073446.1. On or before May 12, 2012 this sequence version replaced XM_001074892.2, XM_343483.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB547496.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGTGG ACAACTGGCT ACTGCACCCC CTCTGGGGAC AGACCTTTCT CCTCCTACTG 
TCTGTGGCTG TGGCTCAAGC CCACTGGCCC AGTGAACCCT CAGAGGCTGT GAGGGACTGG 
AAGAACCAGC TTGAGGCGTC CATGCACTCA GTTCTCTCGG ACTTCCAGGA GGCTGTTCCC 
ACCGTGGTTG GCATCCCAGA TGGCACGGCT GTTGTTGGGC GCTCATTCCG AGTGAGCATT 
CCAACAGATT TAATTGCCTC CAGTGGAGAA ATTATCAAGG TGTCTGCAGC AGGGAAGGAG 
GCCCTGCCGT CCTGGCTACA CTGGGATCCA CACAGTCATA TTTTGGAAGG CCTTCCTCTT 
GACACTGATA AAGGTGTCCA TTACATCTCA GTGAGTGCTG CACGCCTGGG AGCAAATGGA 
AGCCACGTGC CGCAGACGGC CAGCGTGTTC TCTATTGAGG TCTACCCTGA AGACCACAGT 
GAGCCACAGT CTGTCCGGGC AGCCTCATCA GACCCTGGTG AAGTAGTGTC ATCTGCTTGT 
GCAGCTGATG AGCCAGTGAC TGTCCTTACA GTGATTCTGG ATGCTGACCT CACCAAGATG 
ACCCCAAAGC AAAGGATTGA TCTACTGAAC AGAATGCAGA GCTTCTCAGA AGTAGAACTT 
AACAACATGA AGTTGGTGCC TGTAGTCAAT AATAGACTAT TTGACATGTC AGCCTTCATG 
GCTGGCCCAG GAAATGCAAA GAAAGTGGTA GAGAATGGGG CTCTGCTGTC CTGGAAACTA 
GGGTGCTCCT TGAATCAGAA TAGCGTCCCT GACATCCGCG GTGTAGAAAC CCCTGCTAGG 
GAGGGTACCA TGTCTGCCCA CCTTGGTTAT CCTGTGGTGG GTTGGCACAT TGCCAATAAG 
AAGCCCACTC TCCCCAAACG AATCCGGAGG CAGATCCATG CCACTCCTAC ACCTGTTACT 
GCCATTGGAC CCCCAACTAC CGCCATTCAG GAGCCACCAT CTAGGATAGT GCCTACTCCC 
ACATCTCCAG CCATTGCACC TCCAACAGAG ACCATGGCTC CTCCTGTTAG GGATCCTGTC 
CCAGGGAAGC CCACGGTCAC CATTCGGACT CGAGGTGCCA TTATTCAGAC CCCAACTCTG 
GGCCCTATCC CTCCTACTCG GGTGTCAGAA GCTGGTACCA CAGTTCCTGG CCAGATTCGC 
CCAACACTGA CAATTCCTGG TTATGTAGAG CCCACAGCAG TTGTTACTCC TCCAACAACT 
ACCACAAAGA AGCCACGAGT ATCTACGCCA AAACCAGCAA CGCCTTCAAC TGATTCGTCA 
ACTACCACAA CTCGAAGGCC AACCAAAAAA CCACGGACAC CCCGACCAGT GCCCCGGGTC 
ACCACCAAAG CACCGATCAC CAGATTGGAG ACCGCTTCCC CACCCACTCG AATCCGCACT 
ACCACCAGTG CAGTGCCCCG TGGGGGAGAA GCTAACCAGC GGCCAGAGCT TAAGAACCAC 
ATCGACAGGG TTGATGCCTG GGTAGGAACC TACTTTGAGG TAAAGATTCC ATCAGACACC 
TTCTATGACA ATGAGGACAC CACTACGGAC AAGCTCAAGC TGACCCTGAA GCTGCGAGAG 
CAGCAGTTGG TAGGTGAGAA ATCTTGGGTT CAGTTTAACA GCAACAGCCA GCTCATGTAT 
GGTCTGCCTG ACAGCAGCCA TGTCGGAAAA CATGAGTATT TCATGCACGC CACGGACAAA 
GGGGGCCTTT CTGCTGTGGA TGCCTTCGAG ATCCATGTTC ACAAGCGCCC ACAAGGGGAC 
AAAGCTCCCG CGCGATTCAA GGCCAAACTT GCAGGGGATC CCGCACCTGT GGTGAACGAT 
ATCCACAAGA AGATCGCTTT GGTAAAGAAG CTGGCTTTTG CCTTTGGGGA TAGGAACTGC 
AGCTCCATCA CCCTTCAGAA CATCACCCGG GGCTCTATTG TGGTCGAGTG GACCAACAAC 
ACTCTGCCCC TGGAGCCCTG CCCCAAGGAG CAGATCGTAG GGCTGAGCCG CAGGATTGCT 
GATGAAAACG GGAAGCCTCG TCCTGCCTTC TCCAATGCTC TGGAGCCTGA CTTCAAGGCT 
CTGAGTGTTG CTGTGGCGGG CTCTGGCAGT TGTCGGCACC TCCAATTTAT CCCCGTGGCA 
CCACCCTCTC CTGGAACCTC AGCAGCACCG GCCACAGAGG TTCCAGACAG GGACCCTGAG 
AAGAGCAGTG AGGATGACGT TTACTTGCAC ACAGTCATCC CAGCTGTTGT GGTTGCAGCC 
ATCCTGCTCA TTGCTGGAAT CATTGCTATG ATCTGCTATC GCAAGAAGAG GAAGGGCAAA 
CTGACCCTGG AGGACCAGGC CACCTTTATC AAGAAGGGGG TGCCTATTAT CTTTGCAGAT 
GAGCTGGATG ACTCTAAGCC CCCGCCCTCT TCCAGCATGC CGCTCATCCT GCAGGAAGAG 
AAGGCTCCCC TCCCTCCTCC TGAGTACCCT AACCAGAGTG TGCCCGAGAC CACTCCTCTG 
AACCAGGACA CTGTGGGAGA GTACACACCC CTGCGGGATG AGGATCCTAA TGCACCTCCC 
TACCAGCCAC CCCCACCCTT CACAGCTCCC ATGGAGGGCA AGGGCTCCCG TCCCAAGAAC 
ATGACCCCAT ACCGATCACC CCCTCCATAT GTACCCCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446149.1
CDS	120..2801
Translation	MSVDNWLLHPLWGQTFLLLLSVAVAQAHWPSEPSEAVRDWKNQLEASMHSVLSDFQEAVPTVVGIPDGTAVVGRSFRVSIPTDLIASSGEIIKVSAAGKEALPSWLHWDPHSHILEGLPLDTDKGVHYISVSAARLGANGSHVPQTASVFSIEVYPEDHSEPQSVRAASSDPGEVVSSACAADEPVTVLTVILDADLTKMTPKQRIDLLNRMQSFSEVELNNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLNQNSVPDIRGVETPAREGTMSAHLGYPVVGWHIANKKPTLPKRIRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIPPTRVSEAGTTVPGQIRPTLTIPGYVEPTAVVTPPTTTTKKPRVSTPKPATPSTDSSTTTTRRPTKKPRTPRPVPRVTTKAPITRLETASPPTRIRTTTSAVPRGGEANQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHKRPQGDKAPARFKAKLAGDPAPVVNDIHKKIALVKKLAFAFGDRNCSSITLQNITRGSIVVEWTNNTLPLEPCPKEQIVGLSRRIADENGKPRPAFSNALEPDFKALSVAVAGSGSCRHLQFIPVAPPSPGTSAAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSVPETTPLNQDTVGEYTPLRDEDPNAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	NM_053697.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dystroglycan 1 (Dag1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053697.1

ORF Insert Sequence:

ATGTCTGTGG ACAACTGGCT ACTGCACCCC CTCTGGGGAC AGACCTTTCT CCTCCTACTG 
TCTGTGGCTG TGGCTCAAGC CCACTGGCCC AGTGAACCCT CAGAGGCTGT GAGGGACTGG 
AAGAACCAGC TTGAGGCGTC CATGCACTCA GTTCTCTCGG ACTTCCAGGA GGCTGTTCCC 
ACCGTGGTTG GCATCCCAGA TGGCACGGCT GTTGTTGGGC GCTCATTCCG AGTGAGCATT 
CCAACAGATT TAATTGCCTC CAGTGGAGAA ATTATCAAGG TGTCTGCAGC AGGGAAGGAG 
GCCCTGCCGT CCTGGCTACA CTGGGATCCA CACAGTCATA TTTTGGAAGG CCTTCCTCTT 
GACACTGATA AAGGTGTCCA TTACATCTCA GTGAGTGCTG CACGCCTGGG AGCAAATGGA 
AGCCACGTGC CGCAGACGGC CAGCGTGTTC TCTATTGAGG TCTACCCTGA AGACCACAGT 
GAGCCACAGT CTGTCCGGGC AGCCTCATCA GACCCTGGTG AAGTAGTGTC ATCTGCTTGT 
GCAGCTGATG AGCCAGTGAC TGTCCTTACA GTGATTCTGG ATGCTGACCT CACCAAGATG 
ACCCCAAAGC AAAGGATTGA TCTACTGAAC AGAATGCAGA GCTTCTCAGA AGTAGAACTT 
AACAACATGA AGTTGGTGCC TGTAGTCAAT AATAGACTAT TTGACATGTC AGCCTTCATG 
GCTGGCCCAG GAAATGCAAA GAAAGTGGTA GAGAATGGGG CTCTGCTGTC CTGGAAACTA 
GGGTGCTCCT TGAATCAGAA TAGCGTCCCT GACATCCGCG GTGTAGAAAC CCCTGCTAGG 
GAGGGTACCA TGTCTGCCCA CCTTGGTTAT CCTGTGGTGG GTTGGCACAT TGCCAATAAG 
AAGCCCACTC TCCCCAAACG AATCCGGAGG CAGATCCATG CCACTCCTAC ACCTGTTACT 
GCCATTGGAC CCCCAACTAC CGCCATTCAG GAGCCACCAT CTAGGATAGT GCCTACTCCC 
ACATCTCCAG CCATTGCACC TCCAACAGAG ACCATGGCTC CTCCTGTTAG GGATCCTGTC 
CCAGGGAAGC CCACGGTCAC CATTCGGACT CGAGGTGCCA TTATTCAGAC CCCAACTCTG 
GGCCCTATCC CTCCTACTCG GGTGTCAGAA GCTGGTACCA CAGTTCCTGG CCAGATTCGC 
CCAACACTGA CAATTCCTGG TTATGTAGAG CCCACAGCAG TTGTTACTCC TCCAACAACT 
ACCACAAAGA AGCCACGAGT ATCTACGCCA AAACCAGCAA CGCCTTCAAC TGATTCGTCA 
ACTACCACAA CTCGAAGGCC AACCAAAAAA CCACGGACAC CCCGACCAGT GCCCCGGGTC 
ACCACCAAAG CACCGATCAC CAGATTGGAG ACCGCTTCCC CACCCACTCG AATCCGCACT 
ACCACCAGTG CAGTGCCCCG TGGGGGAGAA GCTAACCAGC GGCCAGAGCT TAAGAACCAC 
ATCGACAGGG TTGATGCCTG GGTAGGAACC TACTTTGAGG TAAAGATTCC ATCAGACACC 
TTCTATGACA ATGAGGACAC CACTACGGAC AAGCTCAAGC TGACCCTGAA GCTGCGAGAG 
CAGCAGTTGG TAGGTGAGAA ATCTTGGGTT CAGTTTAACA GCAACAGCCA GCTCATGTAT 
GGTCTGCCTG ACAGCAGCCA TGTCGGAAAA CATGAGTATT TCATGCACGC CACGGACAAA 
GGGGGCCTTT CTGCTGTGGA TGCCTTCGAG ATCCATGTTC ACAAGCGCCC ACAAGGGGAC 
AAAGCTCCCG CGCGATTCAA GGCCAAACTT GCAGGGGATC CCGCACCTGT GGTGAACGAT 
ATCCACAAGA AGATCGCTTT GGTAAAGAAG CTGGCTTTTG CCTTTGGGGA TAGGAACTGC 
AGCTCCATCA CCCTTCAGAA CATCACCCGG GGCTCTATTG TGGTCGAGTG GACCAACAAC 
ACTCTGCCCC TGGAGCCCTG CCCCAAGGAG CAGATCGTAG GGCTGAGCCG CAGGATTGCT 
GATGAAAACG GGAAGCCTCG TCCTGCCTTC TCCAATGCTC TGGAGCCTGA CTTCAAGGCT 
CTGAGTGTTG CTGTGGCGGG CTCTGGCAGT TGTCGGCACC TCCAATTTAT CCCCGTGGCA 
CCACCCTCTC CTGGAACCTC AGCAGCACCG GCCACAGAGG TTCCAGACAG GGACCCTGAG 
AAGAGCAGTG AGGATGACGT TTACTTGCAC ACAGTCATCC CAGCTGTTGT GGTTGCAGCC 
ATCCTGCTCA TTGCTGGAAT CATTGCTATG ATCTGCTATC GCAAGAAGAG GAAGGGCAAA 
CTGACCCTGG AGGACCAGGC CACCTTTATC AAGAAGGGGG TGCCTATTAT CTTTGCAGAT 
GAGCTGGATG ACTCTAAGCC CCCGCCCTCT TCCAGCATGC CGCTCATCCT GCAGGAAGAG 
AAGGCTCCCC TCCCTCCTCC TGAGTACCCT AACCAGAGTG TGCCCGAGAC CACTCCTCTG 
AACCAGGACA CTGTGGGAGA GTACACACCC CTGCGGGATG AGGATCCTAA TGCACCTCCC 
TACCAGCCAC CCCCACCCTT CACAGCTCCC ATGGAGGGCA AGGGCTCCCG TCCCAAGAAC 
ATGACCCCAT ACCGATCACC CCCTCCATAT GTACCCCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neuronal Dystroglycan Is Necessary for Formation and Maintenance of Functional CCK-Positive Basket Cell Terminals on Pyramidal Cells.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(40)10296-10313(2016 10)
Früh S,Romanos J,Panzanelli P,Bürgisser D,Tyagarajan SK,Campbell KP,Santello M,Fritschy JM

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Loss of AQP4 polarized localization with loss of β-dystroglycan immunoreactivity may induce brain edema following intracerebral hemorrhage.
Neuroscience letters58842-8(2015 Feb)
Qiu GP,Xu J,Zhuo F,Sun SQ,Liu H,Yang M,Huang J,Lu WT,Huang SQ

Lost Polarization of Aquaporin4 and Dystroglycan in the Core Lesion after Traumatic Brain Injury Suggests Functional Divergence in Evolution.
BioMed research international2015471631(2015)
Liu H,Qiu Gp,Zhuo F,Yu Wh,Sun Sq,Li Fh,Yang M

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Dag1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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