Vcan cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vcan
Entrez Gene ID	114122
Full Name	versican
Synonyms	Cspg2
General protein information	Preferred Names versican Names versican core protein GHAP PG-M chondroitin sulfate proteoglycan 2 (versican) chondroitin sulfate proteoglycan core protein 2 glial hyaluronate-binding protein large fibroblast proteoglycan
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12
Summary	extracellular matrix protein secreted by many cell types; part of the large chondroitin sulfate proteoglycan (CSPG) family [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053663.1	NP_446115.1	versican core protein isoform 2 precursor
NM_001170560.1	NP_001164031.1	versican core protein isoform 3 precursor
NM_001170558.1	NP_001164029.1	versican core protein isoform 1 precursor
NM_001170559.1	NP_001164030.1	versican core protein isoform 4 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	VCAN	NP_004376.2
Mus musculus (house mouse)	Vcan	NP_001074718.1
Bos taurus (cattle)	VCAN	NP_851378.1
Macaca mulatta (Rhesus monkey)	VCAN	XP_001112269.1
Canis lupus familiaris (dog)	VCAN	XP_003434465.1
Rattus norvegicus (Norway rat)	Vcan	NP_001164029.1
Pan troglodytes (chimpanzee)	VCAN	XP_517667.2

Gene Ontology
Bibliography

Related articles in PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Versican localizes to the nucleus in proliferating mesenchymal cells.
Carthy JM, Abraham T, Meredith AJ, Boroomand S, McManus BM
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology24(6)368-74(2015 Nov-Dec)

Expression of V3 Versican by Rat Arterial Smooth Muscle Cells Promotes Differentiated and Anti-inflammatory Phenotypes.
Kang I, Barth JL, Sproul EP, Yoon DW, Workman GA, Braun KR, Argraves WS, Wight TN
The Journal of biological chemistry290(35)21629-41(2015 Aug)

Neuronally produced versican V2 renders C-fiber nociceptors IB4 -positive.
Bogen O, Bender O, Löwe J, Blenau W, Thevis B, Schröder W, Margolis RU, Levine JD, Hucho F
Journal of neurochemistry134(1)147-55(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vcan gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vcan cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06316	NM_001170559.1 Latest version!	Rattus norvegicus versican (Vcan), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa39882	NM_001170560.1 Latest version!	Rattus norvegicus versican (Vcan), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa10250	NM_053663.1 Latest version!	Rattus norvegicus versican (Vcan), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ORa39880	NM_001170558.1 Latest version!	Rattus norvegicus versican (Vcan), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vcan ( NM_001170559.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001164030.1 Rattus norvegicus versican (Vcan), transcript variant 4, mRNA.

Price & Availability↑

CloneID	ORa06316
Clone ID Related Accession (Same CDS sequence)	NM_001170559.1
Accession Version	NM_001170559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576857600000
Organism	Rattus norvegicus(Norway rat)
Product	versican core protein isoform 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF072892.1 and AY007691.1. Transcript Variant: This variant (4) lacks two alternate in-frame exons, compared to variant 1, resulting in a shorter protein (isoform 4, also referred to as V3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGATAA ATATGAACGG CATCCTATGG ATGTGCTCAA CCTTACTGTT AACGCATGCA 
CTGCATAAAG CCAAAATGGA AGAAAACCCA CCTGTTAAAG GCTCTCTGTC TGGAAAAGTG 
ATCCTACCTT GTCATTTTTC AACCTTGCCC ACCTTACCAC CCGATTACAA CACGAGTGAA 
TTTCTCAGAA TCAAATGGTC TAAAATAGAA GTGGACAAAA ATGGAAAAGA CATAAAGGAG 
ACTACTGTCC TGGTGGCCCA AGACGGGAAC ATCAAGATTG GTCAGGACTA CAAGGGGCGG 
GTATCAGTGC CTACGCATCC CGATGACGTA GGCGATGCCT CTCTCACCAT GGTCAAACTC 
CGTGCTAGTG ACGCAGGTGT CTACCGCTGT GATGTCATGT ATGGCATTGA AGACACTCAG 
AACACGATGT CGCTGGCCGT GGACGGTGTC GTGTTTCACT ACAGGGCAGC GACCAGCAGA 
TACACTCTGA ACTTCGAGTC TGCTCAACAG GCTTGTTTGG ACATCGGGGC GGTCATAGCA 
ACCCCAGAGC AGCTGTTCGC TGCCTATGAG GATGGATTTG AGCAGTGTGA TGCAGGATGG 
CTGTCTGACC AAACTGTCAG ATATCCCATA CGGGCTCCCC GAGAGGGCTG TTATGGAGAC 
ATGATGGGGA AGGAAGGGGT CCGGACCTAT GGATTCCGCT CTCCCCAGGA AACCTATGAT 
GTGTATTGCT ATGTGGATCA TCTGGACGGC GATGTGTTCC ACATCACTGC TCCCAGTAAA 
TTCACCTTCG AGGAGGCCGA AGCAGAGTGT GCAAACCGGG ATGCCAGGCT GGCGACTGTT 
GGGGAACTTC ACGCAGCTTG GAGGAACGGC TTTGACCAGT GCGATTACGG CTGGCTGTCG 
GATGCCAGCG TGCGGCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACATGCT TCCCTCTCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACGACCTGAT CTCTGCAAAA CAAACCCATG CCTCAATGGA 
GGCACCTGCT ATCCTACTGA GACTTCCTAT GTGTGCACCT GTGCACCTGG CTACAGTGGA 
GACCAGTGTG AACTGGATTT TGATGAATGT CACTCTAACC CTTGTCGGAA TGGAGCCACC 
TGTGTGGACG GTCTGAATAC ATTTAGATGC CTCTGCCTTC CGAGTTATGT CGGTGCACTC 
TGCGAACAAG ACACTGAGAC ATGCGACTAT GGCTGGCACA AATTCCAAGG GCAATGCTAC 
AAGTACTTTG CTCATCGCCG TACATGGGAT GCTGCTGAAA GGGAGTGTCG CCTGCAGGGT 
GCCCACCTCA CAAGCATCCT TTCTCATGAG GAACAAATGT TTGTGAATCG TGTGGGCCAT 
GATTACCAGT GGATTGGCCT CAATGACAAG ATGTTTGAAC ATGACTTCCG CTGGACTGAC 
GGCAGCGCAC TGCAATATGA GAACTGGAGA CCCAACCAGC CAGACAGCTT CTTTTCTGCT 
GGAGAAGACT GCGTTGTGAT CATTTGGCAT GAGAATGGCC AGTGGAATGA CGTCCCCTGC 
AACTACCACC TCACCTACAC CTGCAAGAAG GGAACAGTTG CTTGCGGCCA ACCCCCTGTT 
GTAGAAAATG CCAAGACCTT TGGAAAGATG AAACCACGTT ATGAAATCAA CTCCTTGATT 
AGATACCACT GCAAAGATGG TTTCATTCAG CGTCACCTTC CAACTATCCG GTGCCTAGGA 
AATGGGAGAT GGGCAATGCC TAAAATAACC TGCATGAACC CATCTGCATA CCAAAGGACT 
TATTCTAAGA AATACTTAAA AAATTCCTCA TCAGTCAAGG ACAATTCTAT AAATACGTCA 
AAACATGAGC ATCGCTGGAG CCGGAGGTGG CAGGAAACGA GGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164030.1
CDS	281..2248
Translation	MLINMNGILWMCSTLLLTHALHKAKMEENPPVKGSLSGKVILPCHFSTLPTLPPDYNTSEFLRIKWSKIEVDKNGKDIKETTVLVAQDGNIKIGQDYKGRVSVPTHPDDVGDASLTMVKLRASDAGVYRCDVMYGIEDTQNTMSLAVDGVVFHYRAATSRYTLNFESAQQACLDIGAVIATPEQLFAAYEDGFEQCDAGWLSDQTVRYPIRAPREGCYGDMMGKEGVRTYGFRSPQETYDVYCYVDHLDGDVFHITAPSKFTFEEAEAECANRDARLATVGELHAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTCFPLPDSRFDAYCFKRPDLCKTNPCLNGGTCYPTETSYVCTCAPGYSGDQCELDFDECHSNPCRNGATCVDGLNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSALQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAMPKITCMNPSAYQRTYSKKYLKNSSSVKDNSINTSKHEHRWSRRWQETRR

Target ORF information:

RefSeq Version	NM_001170559.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus versican (Vcan), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170559.1

ORF Insert Sequence:

ATGTTGATAA ATATGAACGG CATCCTATGG ATGTGCTCAA CCTTACTGTT AACGCATGCA 
CTGCATAAAG CCAAAATGGA AGAAAACCCA CCTGTTAAAG GCTCTCTGTC TGGAAAAGTG 
ATCCTACCTT GTCATTTTTC AACCTTGCCC ACCTTACCAC CCGATTACAA CACGAGTGAA 
TTTCTCAGAA TCAAATGGTC TAAAATAGAA GTGGACAAAA ATGGAAAAGA CATAAAGGAG 
ACTACTGTCC TGGTGGCCCA AGACGGGAAC ATCAAGATTG GTCAGGACTA CAAGGGGCGG 
GTATCAGTGC CTACGCATCC CGATGACGTA GGCGATGCCT CTCTCACCAT GGTCAAACTC 
CGTGCTAGTG ACGCAGGTGT CTACCGCTGT GATGTCATGT ATGGCATTGA AGACACTCAG 
AACACGATGT CGCTGGCCGT GGACGGTGTC GTGTTTCACT ACAGGGCAGC GACCAGCAGA 
TACACTCTGA ACTTCGAGTC TGCTCAACAG GCTTGTTTGG ACATCGGGGC GGTCATAGCA 
ACCCCAGAGC AGCTGTTCGC TGCCTATGAG GATGGATTTG AGCAGTGTGA TGCAGGATGG 
CTGTCTGACC AAACTGTCAG ATATCCCATA CGGGCTCCCC GAGAGGGCTG TTATGGAGAC 
ATGATGGGGA AGGAAGGGGT CCGGACCTAT GGATTCCGCT CTCCCCAGGA AACCTATGAT 
GTGTATTGCT ATGTGGATCA TCTGGACGGC GATGTGTTCC ACATCACTGC TCCCAGTAAA 
TTCACCTTCG AGGAGGCCGA AGCAGAGTGT GCAAACCGGG ATGCCAGGCT GGCGACTGTT 
GGGGAACTTC ACGCAGCTTG GAGGAACGGC TTTGACCAGT GCGATTACGG CTGGCTGTCG 
GATGCCAGCG TGCGGCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACATGCT TCCCTCTCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACGACCTGAT CTCTGCAAAA CAAACCCATG CCTCAATGGA 
GGCACCTGCT ATCCTACTGA GACTTCCTAT GTGTGCACCT GTGCACCTGG CTACAGTGGA 
GACCAGTGTG AACTGGATTT TGATGAATGT CACTCTAACC CTTGTCGGAA TGGAGCCACC 
TGTGTGGACG GTCTGAATAC ATTTAGATGC CTCTGCCTTC CGAGTTATGT CGGTGCACTC 
TGCGAACAAG ACACTGAGAC ATGCGACTAT GGCTGGCACA AATTCCAAGG GCAATGCTAC 
AAGTACTTTG CTCATCGCCG TACATGGGAT GCTGCTGAAA GGGAGTGTCG CCTGCAGGGT 
GCCCACCTCA CAAGCATCCT TTCTCATGAG GAACAAATGT TTGTGAATCG TGTGGGCCAT 
GATTACCAGT GGATTGGCCT CAATGACAAG ATGTTTGAAC ATGACTTCCG CTGGACTGAC 
GGCAGCGCAC TGCAATATGA GAACTGGAGA CCCAACCAGC CAGACAGCTT CTTTTCTGCT 
GGAGAAGACT GCGTTGTGAT CATTTGGCAT GAGAATGGCC AGTGGAATGA CGTCCCCTGC 
AACTACCACC TCACCTACAC CTGCAAGAAG GGAACAGTTG CTTGCGGCCA ACCCCCTGTT 
GTAGAAAATG CCAAGACCTT TGGAAAGATG AAACCACGTT ATGAAATCAA CTCCTTGATT 
AGATACCACT GCAAAGATGG TTTCATTCAG CGTCACCTTC CAACTATCCG GTGCCTAGGA 
AATGGGAGAT GGGCAATGCC TAAAATAACC TGCATGAACC CATCTGCATA CCAAAGGACT 
TATTCTAAGA AATACTTAAA AAATTCCTCA TCAGTCAAGG ACAATTCTAT AAATACGTCA 
AAACATGAGC ATCGCTGGAG CCGGAGGTGG CAGGAAACGA GGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vcan ( NM_001170560.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001164031.1 Rattus norvegicus versican (Vcan), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa39882
Clone ID Related Accession (Same CDS sequence)	NM_001170560.1
Accession Version	NM_001170560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576857600000
Organism	Rattus norvegicus(Norway rat)
Product	versican core protein isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF072892.1, AABR07007554.1, AF062402.1 and AY007691.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 3, also referred to as V2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data from several strains because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGATAA ATATGAACGG CATCCTATGG ATGTGCTCAA CCTTACTGTT AACGCATGCA 
CTGCATAAAG CCAAAATGGA AGAAAACCCA CCTGTTAAAG GCTCTCTGTC TGGAAAAGTG 
ATCCTACCTT GTCATTTTTC AACCTTGCCC ACCTTACCAC CCGATTACAA CACGAGTGAA 
TTTCTCAGAA TCAAATGGTC TAAAATAGAA GTGGACAAAA ATGGAAAAGA CATAAAGGAG 
ACTACTGTCC TGGTGGCCCA AGACGGGAAC ATCAAGATTG GTCAGGACTA CAAGGGGCGG 
GTATCAGTGC CTACGCATCC CGATGACGTA GGCGATGCCT CTCTCACCAT GGTCAAACTC 
CGTGCTAGTG ACGCAGGTGT CTACCGCTGT GATGTCATGT ATGGCATTGA AGACACTCAG 
AACACGATGT CGCTGGCCGT GGACGGTGTC GTGTTTCACT ACAGGGCAGC GACCAGCAGA 
TACACTCTGA ACTTCGAGTC TGCTCAACAG GCTTGTTTGG ACATCGGGGC GGTCATAGCA 
ACCCCAGAGC AGCTGTTCGC TGCCTATGAG GATGGATTTG AGCAGTGTGA TGCAGGATGG 
CTGTCTGACC AAACTGTCAG ATATCCCATA CGGGCTCCCC GAGAGGGCTG TTATGGAGAC 
ATGATGGGGA AGGAAGGGGT CCGGACCTAT GGATTCCGCT CTCCCCAGGA AACCTATGAT 
GTGTATTGCT ATGTGGATCA TCTGGACGGC GATGTGTTCC ACATCACTGC TCCCAGTAAA 
TTCACCTTCG AGGAGGCCGA AGCAGAGTGT GCAAACCGGG ATGCCAGGCT GGCGACTGTT 
GGGGAACTTC ACGCAGCTTG GAGGAACGGC TTTGACCAGT GCGATTACGG CTGGCTGTCG 
GATGCCAGCG TGCGGCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACATGCT TCCCTCTCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACCTAAACAG AATATATCAG AGGCAACCAC CATCGAAACG 
AATACCCTCG CAGAAACTGC ATCACCCAGT TTATCCAAAG AACCACACAT GGTACCCGAG 
AGAGCTACAC CAGTCATTCC ATTAGTCACC GAATTACCTA TTTTTACCAC ACACTTCCCT 
CCTGCAGGAA ATATAGTCAA TTCTGAACAA AAATCTGTAA TCTACTCTCA GGCTATCACA 
GACAGAGTAG CCACTGAATC ACCCACAGCT GCTGGGAGTC CCATTAACTC TTGGGATGCG 
GATGACTACT TGCCTTCTGG GTCAGGACCA CTTGGAAAGC CAGACATATC TGAAATTAAG 
GAGGAAGGAC TTCAAAGTAC AACTGTCACC TCCCAACATG CTACTGCCTC ACACCATGGA 
ATTACAGAGG ATACGCAAAC ACATGAATCA GTTACACAGA TTGAACAAAT AGAAGTGGGC 
CCCTTGGTAA CATCCATGGA AATCACAAAT CACATTTCTT TCAAGGAATT TCTAGAAACC 
AAAACACCAT TGGAATCCAC AGAAGTGACC CTGGAACAGC ATCAGACCGA CATGCCAACA 
GTTATCACCA GTCCTGAATT GGCAACCACA AGTCACTATG GAGTCACCTT GAGAGAAGAT 
GATAGAGAAG ATATAGCACT GACAGTAAGA TCTGGTCAGA GTACCCGGGT CTTTAGCCAA 
ATACCAGAAG TCATCACGGT ATCAAAGACA TCAGAAGACA CTACCTACTC ACAGCTAGGG 
GACTTAGAAT TCGTTTCAAC AGCAACAATA ACCATGCTTG GCACAGATGG ATCACTGACA 
GATGAAGGGA AAGAACCCCA AACTGATGAT AAGACGGCCG AAGACGAGTT TGGTCAATCA 
CAACCTACAA TCCCCTTCCC ATCACAGCAT CTTACAGAAG TAGAATCACT TCCATATTCT 
GGTGACACAA TATCAGTAGA GAGAATATCC ACAGTTAGTT ATCCATCATT ACAAACAGAT 
GTGACACAAG GAAGAGAGAG AACAGAAGTA CCAAGGCCAG GGTTAAAAAA AGATCCTTAT 
GCAGTTGATG AGATACAAGA AAAGATCACC AAAGATCCAT TTATAGGTAC AATAGAAGAA 
GGCTTCTCTG GAATGTCTCT CTCTACATCT TCCTCAGAGA CTTCAGCGGA AAGGACGGAA 
TCTGTTTCCC CAGCATTGAC AATTGAGAAG TTAACTGTGA AGCCAGCCGT AGCAAGCGAT 
GTGGACGAAA TGACAATCCT GACTAGATTA GAAACAGATG TGCCAACGTC TGACGAAGAT 
GTTACAAGTG CTCATTTGAC CCATAGTACT TTAAATGTGG AAGTGGTCAC AGTATTAAAA 
TGGCCAGGGA ATGAAGACAA TTCAACTTCA AAGCCTTTAC CCCCAACAGA ACGTGCAGGC 
TTTACAAAAT CGCCTCCTGT CTCACTTAGC ACTGTAGGAA TAGTTGGAAA AGATAAAGAA 
ACCCCAAGTT TCACAGATGG GGGAGACGAA TACACTCTTT CCCTAGATGG TACTCCCAAG 
CCATTAGAAA AGTTTTCTGA GGAAGATTTA ACATCTGGAG AATTTGCAGT TACAATTCCT 
ACATCTATAT CAATTGACAG TGCAGAGAAG TCAACTTTGG GAGAGCCTAC AACTGGAGAC 
AGGGTTCTGT CCACCACAAG CACAAAAGAT CTGGTCATCA ATGCAACTGT GGAAGGAAGT 
GCTTTGGATG AAGATATGGA TGCCTCCAAG CCACTGTTTA CTGCCACCCC ATTTGTCCAC 
ACCTCCGATG TGGAAGAATC GGCATTTGTT AATTATAGTA GCACCCAGCA GCCCACTACT 
TATGTAGACA TATCCCACAC TAGTCCTCTT TCTATAATTC CCAAGACAGA ATGGAGTGTA 
TCGGAAACTT CTGTTCCACT GGAAGATGAG GTTCTAGGCA AATCTGATCA AGATACCCTT 
GAACAGACAC ATCTAGAAGC AACCATGTCT CCTGAAGCTT TGAGTACAAT AGAAGTCACA 
CAGGGAGAAA CCCAGGAAGA ACCACAAACA CCAGGAATCC CATTTCCTGC TCTCAGTTCT 
ACTGCAGTTA TGACCAAGGA GACAACAGCA TTTGAAGAAG AGGGGGAGGG GTCAACATAT 
ACCCTCTCTG AAGACAGATT GATGACAGAT TCTGAAATAG TCCCAAGTTT AGAAACAACT 
CCAGTTGGTA CATCTTATCC AGGTGGTGCA ATGACACAGC AGGGAGTAGA AATGGATACA 
ATGGTAACAC AGATGTCAAG CATTAGGCCA ACAGTAGTTT TAAGTACTGA GCCAGAAGTA 
AGTTATGAAG CAGAAGGTAG TAGTCCAATG GAATTTGCAT CGACTTTGAA GCCCTTTGGG 
ACTCAAGTCA CACAGCTTGT GGAAGAAACG ACTGAGGAAG GGAAAAAGAC ACCCTTGGAT 
TATACAGATT TAGGTTCAGG GTTATTTGAA CAACCGAGAG TTACTGAGCT CCCAGATTTT 
TCAATGACTC CAAGTGATAT CAGTGTCTTC ACTGCCATCG ACAGCCTTCA CAGAACCACA 
CCCTTGAGGC CACCTTCGCC ATTCACTGAG GAACCACACA TCTTTGAGAA GGAGCCCAGT 
GAGAAAACAA CAGGTGACAT AATACTTCCT AGAGAATCTG TAACACAGCA CCCTCTGACA 
ACTCTCATGG ATATCATAGC CAAGAAAACA GAATCTGATA TTGATCACGA GTATCACATG 
ACCTCAAAGC CTCCTGTAAT GCAGCCAACA AGACCATCAG TTGTGGAAAG AAAAACAACT 
TCCAAACCTC AAGAGTTGTC TACTTCATCA CCCCCGGCAG GGACCAAATT CCACCCTGAC 
ATAAATGTTT ATATTATTGA GGTCAGAGAA AACAAGACAG GACCTGATCT CTGCAAAACA 
AACCCATGCC TCAATGGAGG CACCTGCTAT CCTACTGAGA CTTCCTATGT GTGCACCTGT 
GCACCTGGCT ACAGTGGAGA CCAGTGTGAA CTGGATTTTG ATGAATGTCA CTCTAACCCT 
TGTCGGAATG GAGCCACCTG TGTGGACGGT CTGAATACAT TTAGATGCCT CTGCCTTCCG 
AGTTATGTCG GTGCACTCTG CGAACAAGAC ACTGAGACAT GCGACTATGG CTGGCACAAA 
TTCCAAGGGC AATGCTACAA GTACTTTGCT CATCGCCGTA CATGGGATGC TGCTGAAAGG 
GAGTGTCGCC TGCAGGGTGC CCACCTCACA AGCATCCTTT CTCATGAGGA ACAAATGTTT 
GTGAATCGTG TGGGCCATGA TTACCAGTGG ATTGGCCTCA ATGACAAGAT GTTTGAACAT 
GACTTCCGCT GGACTGACGG CAGCGCACTG CAATATGAGA ACTGGAGACC CAACCAGCCA 
GACAGCTTCT TTTCTGCTGG AGAAGACTGC GTTGTGATCA TTTGGCATGA GAATGGCCAG 
TGGAATGACG TCCCCTGCAA CTACCACCTC ACCTACACCT GCAAGAAGGG AACAGTTGCT 
TGCGGCCAAC CCCCTGTTGT AGAAAATGCC AAGACCTTTG GAAAGATGAA ACCACGTTAT 
GAAATCAACT CCTTGATTAG ATACCACTGC AAAGATGGTT TCATTCAGCG TCACCTTCCA 
ACTATCCGGT GCCTAGGAAA TGGGAGATGG GCAATGCCTA AAATAACCTG CATGAACCCA 
TCTGCATACC AAAGGACTTA TTCTAAGAAA TACTTAAAAA ATTCCTCATC AGTCAAGGAC 
AATTCTATAA ATACGTCAAA ACATGAGCAT CGCTGGAGCC GGAGGTGGCA GGAAACGAGG 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164031.1
CDS	281..5146
Translation	MLINMNGILWMCSTLLLTHALHKAKMEENPPVKGSLSGKVILPCHFSTLPTLPPDYNTSEFLRIKWSKIEVDKNGKDIKETTVLVAQDGNIKIGQDYKGRVSVPTHPDDVGDASLTMVKLRASDAGVYRCDVMYGIEDTQNTMSLAVDGVVFHYRAATSRYTLNFESAQQACLDIGAVIATPEQLFAAYEDGFEQCDAGWLSDQTVRYPIRAPREGCYGDMMGKEGVRTYGFRSPQETYDVYCYVDHLDGDVFHITAPSKFTFEEAEAECANRDARLATVGELHAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTCFPLPDSRFDAYCFKPKQNISEATTIETNTLAETASPSLSKEPHMVPERATPVIPLVTELPIFTTHFPPAGNIVNSEQKSVIYSQAITDRVATESPTAAGSPINSWDADDYLPSGSGPLGKPDISEIKEEGLQSTTVTSQHATASHHGITEDTQTHESVTQIEQIEVGPLVTSMEITNHISFKEFLETKTPLESTEVTLEQHQTDMPTVITSPELATTSHYGVTLREDDREDIALTVRSGQSTRVFSQIPEVITVSKTSEDTTYSQLGDLEFVSTATITMLGTDGSLTDEGKEPQTDDKTAEDEFGQSQPTIPFPSQHLTEVESLPYSGDTISVERISTVSYPSLQTDVTQGRERTEVPRPGLKKDPYAVDEIQEKITKDPFIGTIEEGFSGMSLSTSSSETSAERTESVSPALTIEKLTVKPAVASDVDEMTILTRLETDVPTSDEDVTSAHLTHSTLNVEVVTVLKWPGNEDNSTSKPLPPTERAGFTKSPPVSLSTVGIVGKDKETPSFTDGGDEYTLSLDGTPKPLEKFSEEDLTSGEFAVTIPTSISIDSAEKSTLGEPTTGDRVLSTTSTKDLVINATVEGSALDEDMDASKPLFTATPFVHTSDVEESAFVNYSSTQQPTTYVDISHTSPLSIIPKTEWSVSETSVPLEDEVLGKSDQDTLEQTHLEATMSPEALSTIEVTQGETQEEPQTPGIPFPALSSTAVMTKETTAFEEEGEGSTYTLSEDRLMTDSEIVPSLETTPVGTSYPGGAMTQQGVEMDTMVTQMSSIRPTVVLSTEPEVSYEAEGSSPMEFASTLKPFGTQVTQLVEETTEEGKKTPLDYTDLGSGLFEQPRVTELPDFSMTPSDISVFTAIDSLHRTTPLRPPSPFTEEPHIFEKEPSEKTTGDIILPRESVTQHPLTTLMDIIAKKTESDIDHEYHMTSKPPVMQPTRPSVVERKTTSKPQELSTSSPPAGTKFHPDINVYIIEVRENKTGPDLCKTNPCLNGGTCYPTETSYVCTCAPGYSGDQCELDFDECHSNPCRNGATCVDGLNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSALQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAMPKITCMNPSAYQRTYSKKYLKNSSSVKDNSINTSKHEHRWSRRWQETRR

Target ORF information:

RefSeq Version	NM_001170560.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus versican (Vcan), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170560.1

ORF Insert Sequence:

ATGTTGATAA ATATGAACGG CATCCTATGG ATGTGCTCAA CCTTACTGTT AACGCATGCA 
CTGCATAAAG CCAAAATGGA AGAAAACCCA CCTGTTAAAG GCTCTCTGTC TGGAAAAGTG 
ATCCTACCTT GTCATTTTTC AACCTTGCCC ACCTTACCAC CCGATTACAA CACGAGTGAA 
TTTCTCAGAA TCAAATGGTC TAAAATAGAA GTGGACAAAA ATGGAAAAGA CATAAAGGAG 
ACTACTGTCC TGGTGGCCCA AGACGGGAAC ATCAAGATTG GTCAGGACTA CAAGGGGCGG 
GTATCAGTGC CTACGCATCC CGATGACGTA GGCGATGCCT CTCTCACCAT GGTCAAACTC 
CGTGCTAGTG ACGCAGGTGT CTACCGCTGT GATGTCATGT ATGGCATTGA AGACACTCAG 
AACACGATGT CGCTGGCCGT GGACGGTGTC GTGTTTCACT ACAGGGCAGC GACCAGCAGA 
TACACTCTGA ACTTCGAGTC TGCTCAACAG GCTTGTTTGG ACATCGGGGC GGTCATAGCA 
ACCCCAGAGC AGCTGTTCGC TGCCTATGAG GATGGATTTG AGCAGTGTGA TGCAGGATGG 
CTGTCTGACC AAACTGTCAG ATATCCCATA CGGGCTCCCC GAGAGGGCTG TTATGGAGAC 
ATGATGGGGA AGGAAGGGGT CCGGACCTAT GGATTCCGCT CTCCCCAGGA AACCTATGAT 
GTGTATTGCT ATGTGGATCA TCTGGACGGC GATGTGTTCC ACATCACTGC TCCCAGTAAA 
TTCACCTTCG AGGAGGCCGA AGCAGAGTGT GCAAACCGGG ATGCCAGGCT GGCGACTGTT 
GGGGAACTTC ACGCAGCTTG GAGGAACGGC TTTGACCAGT GCGATTACGG CTGGCTGTCG 
GATGCCAGCG TGCGGCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACATGCT TCCCTCTCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACCTAAACAG AATATATCAG AGGCAACCAC CATCGAAACG 
AATACCCTCG CAGAAACTGC ATCACCCAGT TTATCCAAAG AACCACACAT GGTACCCGAG 
AGAGCTACAC CAGTCATTCC ATTAGTCACC GAATTACCTA TTTTTACCAC ACACTTCCCT 
CCTGCAGGAA ATATAGTCAA TTCTGAACAA AAATCTGTAA TCTACTCTCA GGCTATCACA 
GACAGAGTAG CCACTGAATC ACCCACAGCT GCTGGGAGTC CCATTAACTC TTGGGATGCG 
GATGACTACT TGCCTTCTGG GTCAGGACCA CTTGGAAAGC CAGACATATC TGAAATTAAG 
GAGGAAGGAC TTCAAAGTAC AACTGTCACC TCCCAACATG CTACTGCCTC ACACCATGGA 
ATTACAGAGG ATACGCAAAC ACATGAATCA GTTACACAGA TTGAACAAAT AGAAGTGGGC 
CCCTTGGTAA CATCCATGGA AATCACAAAT CACATTTCTT TCAAGGAATT TCTAGAAACC 
AAAACACCAT TGGAATCCAC AGAAGTGACC CTGGAACAGC ATCAGACCGA CATGCCAACA 
GTTATCACCA GTCCTGAATT GGCAACCACA AGTCACTATG GAGTCACCTT GAGAGAAGAT 
GATAGAGAAG ATATAGCACT GACAGTAAGA TCTGGTCAGA GTACCCGGGT CTTTAGCCAA 
ATACCAGAAG TCATCACGGT ATCAAAGACA TCAGAAGACA CTACCTACTC ACAGCTAGGG 
GACTTAGAAT TCGTTTCAAC AGCAACAATA ACCATGCTTG GCACAGATGG ATCACTGACA 
GATGAAGGGA AAGAACCCCA AACTGATGAT AAGACGGCCG AAGACGAGTT TGGTCAATCA 
CAACCTACAA TCCCCTTCCC ATCACAGCAT CTTACAGAAG TAGAATCACT TCCATATTCT 
GGTGACACAA TATCAGTAGA GAGAATATCC ACAGTTAGTT ATCCATCATT ACAAACAGAT 
GTGACACAAG GAAGAGAGAG AACAGAAGTA CCAAGGCCAG GGTTAAAAAA AGATCCTTAT 
GCAGTTGATG AGATACAAGA AAAGATCACC AAAGATCCAT TTATAGGTAC AATAGAAGAA 
GGCTTCTCTG GAATGTCTCT CTCTACATCT TCCTCAGAGA CTTCAGCGGA AAGGACGGAA 
TCTGTTTCCC CAGCATTGAC AATTGAGAAG TTAACTGTGA AGCCAGCCGT AGCAAGCGAT 
GTGGACGAAA TGACAATCCT GACTAGATTA GAAACAGATG TGCCAACGTC TGACGAAGAT 
GTTACAAGTG CTCATTTGAC CCATAGTACT TTAAATGTGG AAGTGGTCAC AGTATTAAAA 
TGGCCAGGGA ATGAAGACAA TTCAACTTCA AAGCCTTTAC CCCCAACAGA ACGTGCAGGC 
TTTACAAAAT CGCCTCCTGT CTCACTTAGC ACTGTAGGAA TAGTTGGAAA AGATAAAGAA 
ACCCCAAGTT TCACAGATGG GGGAGACGAA TACACTCTTT CCCTAGATGG TACTCCCAAG 
CCATTAGAAA AGTTTTCTGA GGAAGATTTA ACATCTGGAG AATTTGCAGT TACAATTCCT 
ACATCTATAT CAATTGACAG TGCAGAGAAG TCAACTTTGG GAGAGCCTAC AACTGGAGAC 
AGGGTTCTGT CCACCACAAG CACAAAAGAT CTGGTCATCA ATGCAACTGT GGAAGGAAGT 
GCTTTGGATG AAGATATGGA TGCCTCCAAG CCACTGTTTA CTGCCACCCC ATTTGTCCAC 
ACCTCCGATG TGGAAGAATC GGCATTTGTT AATTATAGTA GCACCCAGCA GCCCACTACT 
TATGTAGACA TATCCCACAC TAGTCCTCTT TCTATAATTC CCAAGACAGA ATGGAGTGTA 
TCGGAAACTT CTGTTCCACT GGAAGATGAG GTTCTAGGCA AATCTGATCA AGATACCCTT 
GAACAGACAC ATCTAGAAGC AACCATGTCT CCTGAAGCTT TGAGTACAAT AGAAGTCACA 
CAGGGAGAAA CCCAGGAAGA ACCACAAACA CCAGGAATCC CATTTCCTGC TCTCAGTTCT 
ACTGCAGTTA TGACCAAGGA GACAACAGCA TTTGAAGAAG AGGGGGAGGG GTCAACATAT 
ACCCTCTCTG AAGACAGATT GATGACAGAT TCTGAAATAG TCCCAAGTTT AGAAACAACT 
CCAGTTGGTA CATCTTATCC AGGTGGTGCA ATGACACAGC AGGGAGTAGA AATGGATACA 
ATGGTAACAC AGATGTCAAG CATTAGGCCA ACAGTAGTTT TAAGTACTGA GCCAGAAGTA 
AGTTATGAAG CAGAAGGTAG TAGTCCAATG GAATTTGCAT CGACTTTGAA GCCCTTTGGG 
ACTCAAGTCA CACAGCTTGT GGAAGAAACG ACTGAGGAAG GGAAAAAGAC ACCCTTGGAT 
TATACAGATT TAGGTTCAGG GTTATTTGAA CAACCGAGAG TTACTGAGCT CCCAGATTTT 
TCAATGACTC CAAGTGATAT CAGTGTCTTC ACTGCCATCG ACAGCCTTCA CAGAACCACA 
CCCTTGAGGC CACCTTCGCC ATTCACTGAG GAACCACACA TCTTTGAGAA GGAGCCCAGT 
GAGAAAACAA CAGGTGACAT AATACTTCCT AGAGAATCTG TAACACAGCA CCCTCTGACA 
ACTCTCATGG ATATCATAGC CAAGAAAACA GAATCTGATA TTGATCACGA GTATCACATG 
ACCTCAAAGC CTCCTGTAAT GCAGCCAACA AGACCATCAG TTGTGGAAAG AAAAACAACT 
TCCAAACCTC AAGAGTTGTC TACTTCATCA CCCCCGGCAG GGACCAAATT CCACCCTGAC 
ATAAATGTTT ATATTATTGA GGTCAGAGAA AACAAGACAG GACCTGATCT CTGCAAAACA 
AACCCATGCC TCAATGGAGG CACCTGCTAT CCTACTGAGA CTTCCTATGT GTGCACCTGT 
GCACCTGGCT ACAGTGGAGA CCAGTGTGAA CTGGATTTTG ATGAATGTCA CTCTAACCCT 
TGTCGGAATG GAGCCACCTG TGTGGACGGT CTGAATACAT TTAGATGCCT CTGCCTTCCG 
AGTTATGTCG GTGCACTCTG CGAACAAGAC ACTGAGACAT GCGACTATGG CTGGCACAAA 
TTCCAAGGGC AATGCTACAA GTACTTTGCT CATCGCCGTA CATGGGATGC TGCTGAAAGG 
GAGTGTCGCC TGCAGGGTGC CCACCTCACA AGCATCCTTT CTCATGAGGA ACAAATGTTT 
GTGAATCGTG TGGGCCATGA TTACCAGTGG ATTGGCCTCA ATGACAAGAT GTTTGAACAT 
GACTTCCGCT GGACTGACGG CAGCGCACTG CAATATGAGA ACTGGAGACC CAACCAGCCA 
GACAGCTTCT TTTCTGCTGG AGAAGACTGC GTTGTGATCA TTTGGCATGA GAATGGCCAG 
TGGAATGACG TCCCCTGCAA CTACCACCTC ACCTACACCT GCAAGAAGGG AACAGTTGCT 
TGCGGCCAAC CCCCTGTTGT AGAAAATGCC AAGACCTTTG GAAAGATGAA ACCACGTTAT 
GAAATCAACT CCTTGATTAG ATACCACTGC AAAGATGGTT TCATTCAGCG TCACCTTCCA 
ACTATCCGGT GCCTAGGAAA TGGGAGATGG GCAATGCCTA AAATAACCTG CATGAACCCA 
TCTGCATACC AAAGGACTTA TTCTAAGAAA TACTTAAAAA ATTCCTCATC AGTCAAGGAC 
AATTCTATAA ATACGTCAAA ACATGAGCAT CGCTGGAGCC GGAGGTGGCA GGAAACGAGG 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vcan ( NM_053663.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446115.1 Rattus norvegicus versican (Vcan), transcript variant 2, mRNA.

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CloneID	ORa10250
Clone ID Related Accession (Same CDS sequence)	NM_053663.1
Accession Version	NM_053663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Rattus norvegicus(Norway rat)
Product	versican core protein isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CH473955.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2, also referred to as V1), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01906348 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGATAA ATATGAACGG CATCCTATGG ATGTGCTCAA CCTTACTGTT AACGCATGCA 
CTGCATAAAG CCAAAATGGA AGAAAACCCA CCTGTTAAAG GCTCTCTGTC TGGAAAAGTG 
ATCCTACCTT GTCATTTTTC AACCTTGCCC ACCTTACCAC CCGATTACAA CACGAGTGAA 
TTTCTCAGAA TCAAATGGTC TAAAATAGAA GTGGACAAAA ATGGAAAAGA CATAAAGGAG 
ACTACTGTCC TGGTGGCCCA AGACGGGAAC ATCAAGATTG GTCAGGACTA CAAGGGGCGG 
GTATCAGTGC CTACGCATCC CGATGACGTA GGCGATGCCT CTCTCACCAT GGTCAAACTC 
CGTGCTAGTG ACGCAGGTGT CTACCGCTGT GATGTCATGT ATGGCATTGA AGACACTCAG 
AACACGATGT CGCTGGCCGT GGACGGTGTC GTGTTTCACT ACAGGGCAGC GACCAGCAGA 
TACACTCTGA ACTTCGAGTC TGCTCAACAG GCTTGTTTGG ACATCGGGGC GGTCATAGCA 
ACCCCAGAGC AGCTGTTCGC TGCCTATGAG GATGGATTTG AGCAGTGTGA TGCAGGATGG 
CTGTCTGACC AAACTGTCAG ATATCCCATA CGGGCTCCCC GAGAGGGCTG TTATGGAGAC 
ATGATGGGGA AGGAAGGGGT CCGGACCTAT GGATTCCGCT CTCCCCAGGA AACCTATGAT 
GTGTATTGCT ATGTGGATCA TCTGGACGGC GATGTGTTCC ACATCACTGC TCCCAGTAAA 
TTCACCTTCG AGGAGGCCGA AGCAGAGTGT GCAAACCGGG ATGCCAGGCT GGCGACTGTT 
GGGGAACTTC ACGCAGCTTG GAGGAACGGC TTTGACCAGT GCGATTACGG CTGGCTATCG 
GATGCCAGCG TGCGGCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACATGCT TCCCTCTCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACGTCGAATG AGTGATATGG TTGTAAATGG TCATCCAATA 
GATTCAGAAT CTAAAGAAGA GGAACCTTGC AGTGAAGAAA CAGATCCACT GCATGATCTG 
TTTGCTGAAA TTTTACCCGA GTTACCAGAT TCCTTCGAAA TAGACATATA TCACAGTGAG 
GAAGATGAAG ATGGAGAGGA GGACTGTGTA AATGCAACTG ATGTAACAAC CACTCCGTCA 
GTGCAGTACA TCACTGGGAA GCCACATGTC ACTACAGTGC CCAAAAACCC AGAAGCTGCA 
GAAGCGAGGC GTGGCCTGTA TGAAAGTGTT GCGCCTTCTC AGAATTTCTC AAATACTTCC 
GCAACTGACA CCCATCAGTT TATACCGGCC GAAACAGAAT TGTCAACTAC CATGCAATTT 
ACCAAATCCA AAGAAGCCAC TGAATTGTTA GAAATCACAT GGAAACCCGA GACCTACCCT 
GAAACACCAG AACACTTTTC AAGTGGTGAG CCTGATGTTT TCCCTACACT GCCATCCCAT 
GATGGTAAAA CCACCAAATG GTCAGAGTTC ATCACAGAGA GCAATCCAAA CACAGAAAAT 
CCAGAACACA AACAACCTAA GCCTATACCT CTGTTTCCTG AAGAGTTTTC AGGAGAGGGT 
GCCATTGACC AAGCATCCCA GCAAACAATC TTTTCCACGG CTACAGAAGT AGCACTTGGT 
AAAGAGACAG ACCAAAGTCC TACCATATCT ACCTCTAGCA TACGTTCAGG TTCTGTGTCA 
GTACATGCCT TGGAGGAAGA TCCAATTGCT CTAACAGGAA TCTCACAGAC TGATGAATCC 
ATGTCTACTG TGGAAAGCTG GGTAGAGATG ACCCCTAGCC AAACTGTAGA GTTCTCTGGG 
AGTTCTTCAG CTCCTACTAT TGAAGGGTCT GGGGAAGTAG AAGAATATAC AAATAAAATT 
TTCAACACGG TAACTGATTT GCCACAGAGA GAGCCTACAG ATACGCTCAT CCCCTTGGAT 
ATGAGCAACA TCATGATCAC AGACCACCAC ATTTATACTC CTGCAACCAC CGCTCCTTTG 
GATTCACAGC TACCTTCTAC AGATGCACGG CCTACACAAT TTGGAATTCA AACCACCACC 
TCTGAGTGGG TTTCCAGTAC ATCTTTTGAA GGAAGGAAAA CAGAGGAAGA CAAAGAAAGA 
GATACAAATG CAGCCCACAC AGGGGAAGTC CAACCAGCGA CAGAGAGGTC AGATCGCTTA 
CTCTTAACTT CAGAATTAGA AAGTTCAAAT GTGGCTGCAT CTAGTCCTTT GGACACCTGG 
GAAGGTTTTG TGCCAGAGAC AACATCAACA GTGTCCGAAA AGGAAATGGC AAACACAACT 
CCTGTTTTTA CAGAAACAAG TGATGTAGCC AATTTGGAGA CACAGTCTTT TGAGCATAGT 
AGTAGCAGTC AGCCCAGGGT ACAGGAAGAG CTGACCACTC TCTCAGGAAA GCCCCCCTTG 
ATTTTTATGG ACCTGGGTTC AGGAGATGCT AGTACTGATA TGGAATTCAT CACTGCTTCT 
TCATTTACAT TAGATTTGGA GTCCGATACC AAAGTCAAAA AAGAATTACC CAGTACTCTG 
TCTCCCAGTG TGGAGACTTC ATCCTCCTCT GAGCCAATAG GATTAGCTCC GAGTACTGTA 
TTGGACATAG AAATTGTGGA AGTTATGAAT CAGACATCCA AGAAGACACT GATTTCTGAA 
CTATCAGGAA AGCCAACCTC CCAAGCAGAA GTAAGGGATT TATATCCAGG TCTGGGAGAA 
GATTTTAGTG GTGACTCCAG TGAATATCCA ACAGTGTCTT CTACAACAAT GAAAGAGGAA 
ACAGTGGGGA TGGGAGGCTC TGAGAATGAA CGAGTTAAGG ATACACAGAC TTTATCATCT 
ATACCACCTA CCTCAGACAA CATCAACCCA GTGCCTGACT CAAAAGGATT CGGCAGTACT 
GTGGCCAGCA CCACAGCCTT CCCTTGGGAA GAGTTCATGA CCTCAGCTGA GGGCTCAGGT 
GAGGAGCTGT CCTCAGTCAG GAGCTCTGTT AGTCTAGTGC TTCCACTAGG TGTGGACATT 
CTTCCTACTA CAGAGTCTCC ATATTTTGAT CAAGAGTTTG AAGAAGCGGC TGCAGTGACA 
GAAGCAGGTA AACAATCTGC TTTGCCAATA GCAGTGAGTG GAAATACTGT AGATCTGACA 
GAAAACAGGG ACATAGAGGT AAACAGCACA ATGTCAGTAG ACCTTCCCCA GACAATGGAG 
CCAGCCAAAT TATGGTCTAA ACCAGAAGTC AATCCTGAAA AACAAGAGAT TGGAAGCGAA 
ACAGTGACAC AGGATAAAGC TCAGGGACAA AAGTCTTTTG AATCGCTCCA CAGTTCTCTT 
GCACCAGAAC AAACAACTTT GGAAAGCCAA TCACTTATTG AAACTGAAGT CCAAACTTCA 
TATTACTCTA TGTTAACAAC AATGAAAACA TACAATACTA ATGAGGAAGT GGAGGAGGAA 
GGCACGTCCA TAGCTCATAT GTCTACTCCA GGCCCGGGAA TCAAAGGCTT GGAATCATAT 
CCTACCCATC CTGAAGCTAC TGGGAAGTCA TACTCTTTCT CAGCTTCTGC CTTAGTGACT 
GAATCTGGAC CAGCTAGAAG TGTAGTCATG GATTCCTCAA CTCAAGAGGA AGAAAGTATA 
AAACTCTTCC AAAAGGACAT GATACTAACA CATAAAGAGT CAAATTCTGA TCTCTCGTTC 
TCTGGCCTGG GATCAGGAGA GGCTTTGCCT CCTCTCCCTA CAACATCAGT GAGTTTGACT 
GACATGGGAA AGATCAATAG CACATTGTAT CCTGAGACAT CTCACATGGA AAGCTTAGGA 
ACAAGCATCT TAGGAGATAA CCATGAAAGA ATGAAAAATG TGTCAAATGA AGTTAGAACC 
CTTATTTCCG AAACAGGCAG CATTTCTCAA GACAGTACGG AGGCACCCAA TACAACTTTG 
AGTGATACCA GAACAGAAGA ATCTACAACT TCACCTCTTC CTTTCATGAA GCTCATGGAC 
ACGGAACATT CTCCAAAGCA GACCCTCAGG TGGGAAGAGG AAATCCAGAC CCATAGGCCA 
CAAACTATGA CTGGACAGAT TACCAATGAC AATTCTTCAG TGTCAGAAGC TGAAGCAGCA 
GCAACCTCTG CCCCTGCTTT TCTGCCTGAA ACCTACAGTG TTGAAATGAC CAAAGCCTTT 
GCTACATCAC TGTCACAAAC ATCTGACTTG TTTGATGCAA ATTCTGGAGA AGGATCTGGA 
GAAGTGGATG GTCTTGACTT AGTGTACACT TCTAGAACTA CTCAGGCAAG CAGTCAAGGA 
GACAGCATGT TTGCCTCTCA TGGATTCATT GAAAAGCATC CAGAGGTGTC AAGGACCGAG 
ACTGGAGCTA CTGATGGATC TCCAACAGCT TCAGCGATGT TCCTGCATCA GTCAGAGTAC 
AATGAGAGTT CCCTGTATCC AACCAGCACA CTGCCAAGCA CAGTGACATA TGAGAGTCCC 
TCAGAAGGTA TTGCAGATGG TTTGCAAGAC CATATCAGAT TCGAAGTTTC TACCTTGAAA 
CCTAGCAGAA GGAAAGCCAC TGAGAGCGTT ATTATAGATT TAGACAAAGA GGATAGTAAG 
GACTTAGGGT TGGCAATTAC TGAGAGTGCT ATTGTTGAAA TTCTCCCTGA GCTGACATCA 
GATAGAAATA TTATTATAGA CATTGATCAC ACTAAACCTG TATATGAGTA CATCCCTGGA 
ATACAAACAG ACCTAGATTC AGACATACCA TTGGGATCAC ATGGCAGTAG TGAAGAAAGC 
CTTGAAGTTC AAGAGAAGTA TGAAGCAACT ATTAACCTGT CTCCAACTGA GGAAGCATTT 
GACGGCTCTG GTGATGCACT TCCTGCTGGT CACACTCAAG CGATATACAA TGAGTCTGTG 
ACTCCCAGTG ATGGAAAGCA GCCAGAGGAC ATAAGCTTTA GTTTTGCAAC TGGAATCCCA 
GTTTCTAGCA CAGAGACAGA ATTAAATACT TTCTTCCCTA CAGTGTCAAC CCTGCACATT 
CCTAGCAAAC TAACCACCGC TAGTCCAGAG ATTGATAAAC CCAACATTGA AGCAATATCA 
CTGGATGATA TATTTGAATC AAGCACCTTG TCTGATGGCC AAGCTATTGC CGATCAGAGT 
GAAGTAATAT CAACACTAGG CCATTTGGAA AAGACACAGG AAGAGTATGA AGAAAAGAAA 
TATGGAGGTC CTTCTTTTCA GCCAGAATTC TTTTCAGGAG TGGGTGAAGT ATTCACAGAT 
GCCCCTGCTT ATGTAAGTAT TGGTAGGACA TACTCTGTAG CTCAGCCTTT AACAGAATTC 
CCCAATGTGG TAGGACAATC TGACTCCACA CATTACACTG AAGCAACATC TGCAGTTTCC 
TCTGTGACAG AGCTGTCTCC TCAGACACCA TCTTCTCCCT CTCCTGTCTA CATAGACAGT 
GGAGTCTCTG AATTCACAGA GGTCCCCCAC AAAAGTGCTC AGCCAGCTCC TACTGCCGCT 
TCATCACAAA AGTTGATAGA GGGTTCTTTT AAGGAAGTGC GTGCTAATAT TGAAGCTACC 
ATCAAGTCGC TGGGTGAAAA TGATCACGGA ACAGAGTCTC CATCCATGTC TCCTAGCCCT 
GCTTTAGACA TTTCAGAAGA CGACAGTAAA CCTAAGTTAC TAGAAGACCT GGAAACTTCT 
CCCACAAAAA CCGAGACTTC CCAAGATTCC CCAAACAAAG CAAATGATCA AATTCCAGGA 
AAAACAGCTG GAATCCTTGC TGGTATCAAA ACAACAGAGA GTGGACCAGT CGTTACAGCT 
GCAGATGACA TGGAGCTAGG AGATGCCACA CAGCGGCCAC ATTCTGCTTC TGCTCCTGCA 
GCTTTCAGGG TTGAGACAAG TATGGTACCT CAGCCCATCC CACAGGAGCC TGAGAGGCCA 
ACCTTTCCTT CCCTGGAAAT TAACCATGAA ACACACACAT CATTATTTGA AGAATCTATA 
TTGGCAACAT CTGAAAAACA AGTGTCCCAA AGAATTCTTG ATTACAGTAA CCAGGCAACA 
GTCAGTACTC TGGATCTAAA TACTGAACAC TCAATACCAC CATTTTCCAT TCTGGACAAT 
TCTAACGAAA CTGCTTTCCT GATTGGCATT AGTGAAGAGA CCGTGGAAGG CACAGCAGTT 
TATCTACCAG GACCTGATCT CTGCAAAACA AACCCATGCC TCAATGGAGG CACCTGCTAT 
CCTACTGAGA CTTCCTATGT GTGCACCTGT GCACCTGGCT ACAGTGGAGA CCAGTGTGAA 
CTGGATTTTG ATGAATGTCA CTCTAACCCT TGTCGGAATG GAGCCACCTG TGTGGACGGT 
CTGAATACAT TTAGATGCCT CTGCCTTCCG AGTTATGTCG GTGCACTCTG CGAACAAGAC 
ACTGAGACAT GCGACTATGG CTGGCACAAA TTCCAAGGGC AATGCTACAA GTACTTTGCT 
CATCGCCGTA CATGGGATGC TGCTGAAAGG GAGTGTCGCC TGCAGGGTGC CCACCTCACA 
AGCATCCTTT CTCATGAGGA ACAAATGTTT GTGAATCGTG TGGGCCATGA TTACCAGTGG 
ATTGGCCTCA ATGACAAGAT GTTTGAACAT GACTTCCGCT GGACTGACGG CAGCGCACTG 
CAATATGAGA ACTGGAGACC CAACCAGCCA GACAGCTTCT TTTCTGCTGG AGAAGACTGC 
GTTGTGATCA TTTGGCATGA GAATGGCCAG TGGAATGACG TCCCCTGCAA CTACCACCTC 
ACCTACACCT GCAAGAAGGG AACAGTTGCT TGCGGCCAAC CCCCTGTTGT AGAAAATGCC 
AAGACCTTTG GAAAGATGAA ACCACGTTAT GAAATCAACT CCTTGATTAG ATACCACTGC 
AAAGATGGTT TCATTCAGCG TCACCTTCCA ACTATCCGGT GCCTAGGAAA TGGGAGATGG 
GCAATGCCTA AAATAACCTG CATGAACCCA TCTGCATACC AAAGGACTTA TTCTAAGAAA 
TACTTAAAAA ATTCCTCATC AGTCAAGGAC AATTCTATAA ATACGTCAAA ACATGAGCAT 
CGCTGGAGCC GGAGGTGGCA GGAAACGAGG CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446115.1
CDS	379..7554
Translation	MLINMNGILWMCSTLLLTHALHKAKMEENPPVKGSLSGKVILPCHFSTLPTLPPDYNTSEFLRIKWSKIEVDKNGKDIKETTVLVAQDGNIKIGQDYKGRVSVPTHPDDVGDASLTMVKLRASDAGVYRCDVMYGIEDTQNTMSLAVDGVVFHYRAATSRYTLNFESAQQACLDIGAVIATPEQLFAAYEDGFEQCDAGWLSDQTVRYPIRAPREGCYGDMMGKEGVRTYGFRSPQETYDVYCYVDHLDGDVFHITAPSKFTFEEAEAECANRDARLATVGELHAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTCFPLPDSRFDAYCFKRRMSDMVVNGHPIDSESKEEEPCSEETDPLHDLFAEILPELPDSFEIDIYHSEEDEDGEEDCVNATDVTTTPSVQYITGKPHVTTVPKNPEAAEARRGLYESVAPSQNFSNTSATDTHQFIPAETELSTTMQFTKSKEATELLEITWKPETYPETPEHFSSGEPDVFPTLPSHDGKTTKWSEFITESNPNTENPEHKQPKPIPLFPEEFSGEGAIDQASQQTIFSTATEVALGKETDQSPTISTSSIRSGSVSVHALEEDPIALTGISQTDESMSTVESWVEMTPSQTVEFSGSSSAPTIEGSGEVEEYTNKIFNTVTDLPQREPTDTLIPLDMSNIMITDHHIYTPATTAPLDSQLPSTDARPTQFGIQTTTSEWVSSTSFEGRKTEEDKERDTNAAHTGEVQPATERSDRLLLTSELESSNVAASSPLDTWEGFVPETTSTVSEKEMANTTPVFTETSDVANLETQSFEHSSSSQPRVQEELTTLSGKPPLIFMDLGSGDASTDMEFITASSFTLDLESDTKVKKELPSTLSPSVETSSSSEPIGLAPSTVLDIEIVEVMNQTSKKTLISELSGKPTSQAEVRDLYPGLGEDFSGDSSEYPTVSSTTMKEETVGMGGSENERVKDTQTLSSIPPTSDNINPVPDSKGFGSTVASTTAFPWEEFMTSAEGSGEELSSVRSSVSLVLPLGVDILPTTESPYFDQEFEEAAAVTEAGKQSALPIAVSGNTVDLTENRDIEVNSTMSVDLPQTMEPAKLWSKPEVNPEKQEIGSETVTQDKAQGQKSFESLHSSLAPEQTTLESQSLIETEVQTSYYSMLTTMKTYNTNEEVEEEGTSIAHMSTPGPGIKGLESYPTHPEATGKSYSFSASALVTESGPARSVVMDSSTQEEESIKLFQKDMILTHKESNSDLSFSGLGSGEALPPLPTTSVSLTDMGKINSTLYPETSHMESLGTSILGDNHERMKNVSNEVRTLISETGSISQDSTEAPNTTLSDTRTEESTTSPLPFMKLMDTEHSPKQTLRWEEEIQTHRPQTMTGQITNDNSSVSEAEAAATSAPAFLPETYSVEMTKAFATSLSQTSDLFDANSGEGSGEVDGLDLVYTSRTTQASSQGDSMFASHGFIEKHPEVSRTETGATDGSPTASAMFLHQSEYNESSLYPTSTLPSTVTYESPSEGIADGLQDHIRFEVSTLKPSRRKATESVIIDLDKEDSKDLGLAITESAIVEILPELTSDRNIIIDIDHTKPVYEYIPGIQTDLDSDIPLGSHGSSEESLEVQEKYEATINLSPTEEAFDGSGDALPAGHTQAIYNESVTPSDGKQPEDISFSFATGIPVSSTETELNTFFPTVSTLHIPSKLTTASPEIDKPNIEAISLDDIFESSTLSDGQAIADQSEVISTLGHLEKTQEEYEEKKYGGPSFQPEFFSGVGEVFTDAPAYVSIGRTYSVAQPLTEFPNVVGQSDSTHYTEATSAVSSVTELSPQTPSSPSPVYIDSGVSEFTEVPHKSAQPAPTAASSQKLIEGSFKEVRANIEATIKSLGENDHGTESPSMSPSPALDISEDDSKPKLLEDLETSPTKTETSQDSPNKANDQIPGKTAGILAGIKTTESGPVVTAADDMELGDATQRPHSASAPAAFRVETSMVPQPIPQEPERPTFPSLEINHETHTSLFEESILATSEKQVSQRILDYSNQATVSTLDLNTEHSIPPFSILDNSNETAFLIGISEETVEGTAVYLPGPDLCKTNPCLNGGTCYPTETSYVCTCAPGYSGDQCELDFDECHSNPCRNGATCVDGLNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSALQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAMPKITCMNPSAYQRTYSKKYLKNSSSVKDNSINTSKHEHRWSRRWQETRR

Target ORF information:

RefSeq Version	NM_053663.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus versican (Vcan), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053663.1

ORF Insert Sequence:

ATGTTGATAA ATATGAACGG CATCCTATGG ATGTGCTCAA CCTTACTGTT AACGCATGCA 
CTGCATAAAG CCAAAATGGA AGAAAACCCA CCTGTTAAAG GCTCTCTGTC TGGAAAAGTG 
ATCCTACCTT GTCATTTTTC AACCTTGCCC ACCTTACCAC CCGATTACAA CACGAGTGAA 
TTTCTCAGAA TCAAATGGTC TAAAATAGAA GTGGACAAAA ATGGAAAAGA CATAAAGGAG 
ACTACTGTCC TGGTGGCCCA AGACGGGAAC ATCAAGATTG GTCAGGACTA CAAGGGGCGG 
GTATCAGTGC CTACGCATCC CGATGACGTA GGCGATGCCT CTCTCACCAT GGTCAAACTC 
CGTGCTAGTG ACGCAGGTGT CTACCGCTGT GATGTCATGT ATGGCATTGA AGACACTCAG 
AACACGATGT CGCTGGCCGT GGACGGTGTC GTGTTTCACT ACAGGGCAGC GACCAGCAGA 
TACACTCTGA ACTTCGAGTC TGCTCAACAG GCTTGTTTGG ACATCGGGGC GGTCATAGCA 
ACCCCAGAGC AGCTGTTCGC TGCCTATGAG GATGGATTTG AGCAGTGTGA TGCAGGATGG 
CTGTCTGACC AAACTGTCAG ATATCCCATA CGGGCTCCCC GAGAGGGCTG TTATGGAGAC 
ATGATGGGGA AGGAAGGGGT CCGGACCTAT GGATTCCGCT CTCCCCAGGA AACCTATGAT 
GTGTATTGCT ATGTGGATCA TCTGGACGGC GATGTGTTCC ACATCACTGC TCCCAGTAAA 
TTCACCTTCG AGGAGGCCGA AGCAGAGTGT GCAAACCGGG ATGCCAGGCT GGCGACTGTT 
GGGGAACTTC ACGCAGCTTG GAGGAACGGC TTTGACCAGT GCGATTACGG CTGGCTATCG 
GATGCCAGCG TGCGGCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACATGCT TCCCTCTCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACGTCGAATG AGTGATATGG TTGTAAATGG TCATCCAATA 
GATTCAGAAT CTAAAGAAGA GGAACCTTGC AGTGAAGAAA CAGATCCACT GCATGATCTG 
TTTGCTGAAA TTTTACCCGA GTTACCAGAT TCCTTCGAAA TAGACATATA TCACAGTGAG 
GAAGATGAAG ATGGAGAGGA GGACTGTGTA AATGCAACTG ATGTAACAAC CACTCCGTCA 
GTGCAGTACA TCACTGGGAA GCCACATGTC ACTACAGTGC CCAAAAACCC AGAAGCTGCA 
GAAGCGAGGC GTGGCCTGTA TGAAAGTGTT GCGCCTTCTC AGAATTTCTC AAATACTTCC 
GCAACTGACA CCCATCAGTT TATACCGGCC GAAACAGAAT TGTCAACTAC CATGCAATTT 
ACCAAATCCA AAGAAGCCAC TGAATTGTTA GAAATCACAT GGAAACCCGA GACCTACCCT 
GAAACACCAG AACACTTTTC AAGTGGTGAG CCTGATGTTT TCCCTACACT GCCATCCCAT 
GATGGTAAAA CCACCAAATG GTCAGAGTTC ATCACAGAGA GCAATCCAAA CACAGAAAAT 
CCAGAACACA AACAACCTAA GCCTATACCT CTGTTTCCTG AAGAGTTTTC AGGAGAGGGT 
GCCATTGACC AAGCATCCCA GCAAACAATC TTTTCCACGG CTACAGAAGT AGCACTTGGT 
AAAGAGACAG ACCAAAGTCC TACCATATCT ACCTCTAGCA TACGTTCAGG TTCTGTGTCA 
GTACATGCCT TGGAGGAAGA TCCAATTGCT CTAACAGGAA TCTCACAGAC TGATGAATCC 
ATGTCTACTG TGGAAAGCTG GGTAGAGATG ACCCCTAGCC AAACTGTAGA GTTCTCTGGG 
AGTTCTTCAG CTCCTACTAT TGAAGGGTCT GGGGAAGTAG AAGAATATAC AAATAAAATT 
TTCAACACGG TAACTGATTT GCCACAGAGA GAGCCTACAG ATACGCTCAT CCCCTTGGAT 
ATGAGCAACA TCATGATCAC AGACCACCAC ATTTATACTC CTGCAACCAC CGCTCCTTTG 
GATTCACAGC TACCTTCTAC AGATGCACGG CCTACACAAT TTGGAATTCA AACCACCACC 
TCTGAGTGGG TTTCCAGTAC ATCTTTTGAA GGAAGGAAAA CAGAGGAAGA CAAAGAAAGA 
GATACAAATG CAGCCCACAC AGGGGAAGTC CAACCAGCGA CAGAGAGGTC AGATCGCTTA 
CTCTTAACTT CAGAATTAGA AAGTTCAAAT GTGGCTGCAT CTAGTCCTTT GGACACCTGG 
GAAGGTTTTG TGCCAGAGAC AACATCAACA GTGTCCGAAA AGGAAATGGC AAACACAACT 
CCTGTTTTTA CAGAAACAAG TGATGTAGCC AATTTGGAGA CACAGTCTTT TGAGCATAGT 
AGTAGCAGTC AGCCCAGGGT ACAGGAAGAG CTGACCACTC TCTCAGGAAA GCCCCCCTTG 
ATTTTTATGG ACCTGGGTTC AGGAGATGCT AGTACTGATA TGGAATTCAT CACTGCTTCT 
TCATTTACAT TAGATTTGGA GTCCGATACC AAAGTCAAAA AAGAATTACC CAGTACTCTG 
TCTCCCAGTG TGGAGACTTC ATCCTCCTCT GAGCCAATAG GATTAGCTCC GAGTACTGTA 
TTGGACATAG AAATTGTGGA AGTTATGAAT CAGACATCCA AGAAGACACT GATTTCTGAA 
CTATCAGGAA AGCCAACCTC CCAAGCAGAA GTAAGGGATT TATATCCAGG TCTGGGAGAA 
GATTTTAGTG GTGACTCCAG TGAATATCCA ACAGTGTCTT CTACAACAAT GAAAGAGGAA 
ACAGTGGGGA TGGGAGGCTC TGAGAATGAA CGAGTTAAGG ATACACAGAC TTTATCATCT 
ATACCACCTA CCTCAGACAA CATCAACCCA GTGCCTGACT CAAAAGGATT CGGCAGTACT 
GTGGCCAGCA CCACAGCCTT CCCTTGGGAA GAGTTCATGA CCTCAGCTGA GGGCTCAGGT 
GAGGAGCTGT CCTCAGTCAG GAGCTCTGTT AGTCTAGTGC TTCCACTAGG TGTGGACATT 
CTTCCTACTA CAGAGTCTCC ATATTTTGAT CAAGAGTTTG AAGAAGCGGC TGCAGTGACA 
GAAGCAGGTA AACAATCTGC TTTGCCAATA GCAGTGAGTG GAAATACTGT AGATCTGACA 
GAAAACAGGG ACATAGAGGT AAACAGCACA ATGTCAGTAG ACCTTCCCCA GACAATGGAG 
CCAGCCAAAT TATGGTCTAA ACCAGAAGTC AATCCTGAAA AACAAGAGAT TGGAAGCGAA 
ACAGTGACAC AGGATAAAGC TCAGGGACAA AAGTCTTTTG AATCGCTCCA CAGTTCTCTT 
GCACCAGAAC AAACAACTTT GGAAAGCCAA TCACTTATTG AAACTGAAGT CCAAACTTCA 
TATTACTCTA TGTTAACAAC AATGAAAACA TACAATACTA ATGAGGAAGT GGAGGAGGAA 
GGCACGTCCA TAGCTCATAT GTCTACTCCA GGCCCGGGAA TCAAAGGCTT GGAATCATAT 
CCTACCCATC CTGAAGCTAC TGGGAAGTCA TACTCTTTCT CAGCTTCTGC CTTAGTGACT 
GAATCTGGAC CAGCTAGAAG TGTAGTCATG GATTCCTCAA CTCAAGAGGA AGAAAGTATA 
AAACTCTTCC AAAAGGACAT GATACTAACA CATAAAGAGT CAAATTCTGA TCTCTCGTTC 
TCTGGCCTGG GATCAGGAGA GGCTTTGCCT CCTCTCCCTA CAACATCAGT GAGTTTGACT 
GACATGGGAA AGATCAATAG CACATTGTAT CCTGAGACAT CTCACATGGA AAGCTTAGGA 
ACAAGCATCT TAGGAGATAA CCATGAAAGA ATGAAAAATG TGTCAAATGA AGTTAGAACC 
CTTATTTCCG AAACAGGCAG CATTTCTCAA GACAGTACGG AGGCACCCAA TACAACTTTG 
AGTGATACCA GAACAGAAGA ATCTACAACT TCACCTCTTC CTTTCATGAA GCTCATGGAC 
ACGGAACATT CTCCAAAGCA GACCCTCAGG TGGGAAGAGG AAATCCAGAC CCATAGGCCA 
CAAACTATGA CTGGACAGAT TACCAATGAC AATTCTTCAG TGTCAGAAGC TGAAGCAGCA 
GCAACCTCTG CCCCTGCTTT TCTGCCTGAA ACCTACAGTG TTGAAATGAC CAAAGCCTTT 
GCTACATCAC TGTCACAAAC ATCTGACTTG TTTGATGCAA ATTCTGGAGA AGGATCTGGA 
GAAGTGGATG GTCTTGACTT AGTGTACACT TCTAGAACTA CTCAGGCAAG CAGTCAAGGA 
GACAGCATGT TTGCCTCTCA TGGATTCATT GAAAAGCATC CAGAGGTGTC AAGGACCGAG 
ACTGGAGCTA CTGATGGATC TCCAACAGCT TCAGCGATGT TCCTGCATCA GTCAGAGTAC 
AATGAGAGTT CCCTGTATCC AACCAGCACA CTGCCAAGCA CAGTGACATA TGAGAGTCCC 
TCAGAAGGTA TTGCAGATGG TTTGCAAGAC CATATCAGAT TCGAAGTTTC TACCTTGAAA 
CCTAGCAGAA GGAAAGCCAC TGAGAGCGTT ATTATAGATT TAGACAAAGA GGATAGTAAG 
GACTTAGGGT TGGCAATTAC TGAGAGTGCT ATTGTTGAAA TTCTCCCTGA GCTGACATCA 
GATAGAAATA TTATTATAGA CATTGATCAC ACTAAACCTG TATATGAGTA CATCCCTGGA 
ATACAAACAG ACCTAGATTC AGACATACCA TTGGGATCAC ATGGCAGTAG TGAAGAAAGC 
CTTGAAGTTC AAGAGAAGTA TGAAGCAACT ATTAACCTGT CTCCAACTGA GGAAGCATTT 
GACGGCTCTG GTGATGCACT TCCTGCTGGT CACACTCAAG CGATATACAA TGAGTCTGTG 
ACTCCCAGTG ATGGAAAGCA GCCAGAGGAC ATAAGCTTTA GTTTTGCAAC TGGAATCCCA 
GTTTCTAGCA CAGAGACAGA ATTAAATACT TTCTTCCCTA CAGTGTCAAC CCTGCACATT 
CCTAGCAAAC TAACCACCGC TAGTCCAGAG ATTGATAAAC CCAACATTGA AGCAATATCA 
CTGGATGATA TATTTGAATC AAGCACCTTG TCTGATGGCC AAGCTATTGC CGATCAGAGT 
GAAGTAATAT CAACACTAGG CCATTTGGAA AAGACACAGG AAGAGTATGA AGAAAAGAAA 
TATGGAGGTC CTTCTTTTCA GCCAGAATTC TTTTCAGGAG TGGGTGAAGT ATTCACAGAT 
GCCCCTGCTT ATGTAAGTAT TGGTAGGACA TACTCTGTAG CTCAGCCTTT AACAGAATTC 
CCCAATGTGG TAGGACAATC TGACTCCACA CATTACACTG AAGCAACATC TGCAGTTTCC 
TCTGTGACAG AGCTGTCTCC TCAGACACCA TCTTCTCCCT CTCCTGTCTA CATAGACAGT 
GGAGTCTCTG AATTCACAGA GGTCCCCCAC AAAAGTGCTC AGCCAGCTCC TACTGCCGCT 
TCATCACAAA AGTTGATAGA GGGTTCTTTT AAGGAAGTGC GTGCTAATAT TGAAGCTACC 
ATCAAGTCGC TGGGTGAAAA TGATCACGGA ACAGAGTCTC CATCCATGTC TCCTAGCCCT 
GCTTTAGACA TTTCAGAAGA CGACAGTAAA CCTAAGTTAC TAGAAGACCT GGAAACTTCT 
CCCACAAAAA CCGAGACTTC CCAAGATTCC CCAAACAAAG CAAATGATCA AATTCCAGGA 
AAAACAGCTG GAATCCTTGC TGGTATCAAA ACAACAGAGA GTGGACCAGT CGTTACAGCT 
GCAGATGACA TGGAGCTAGG AGATGCCACA CAGCGGCCAC ATTCTGCTTC TGCTCCTGCA 
GCTTTCAGGG TTGAGACAAG TATGGTACCT CAGCCCATCC CACAGGAGCC TGAGAGGCCA 
ACCTTTCCTT CCCTGGAAAT TAACCATGAA ACACACACAT CATTATTTGA AGAATCTATA 
TTGGCAACAT CTGAAAAACA AGTGTCCCAA AGAATTCTTG ATTACAGTAA CCAGGCAACA 
GTCAGTACTC TGGATCTAAA TACTGAACAC TCAATACCAC CATTTTCCAT TCTGGACAAT 
TCTAACGAAA CTGCTTTCCT GATTGGCATT AGTGAAGAGA CCGTGGAAGG CACAGCAGTT 
TATCTACCAG GACCTGATCT CTGCAAAACA AACCCATGCC TCAATGGAGG CACCTGCTAT 
CCTACTGAGA CTTCCTATGT GTGCACCTGT GCACCTGGCT ACAGTGGAGA CCAGTGTGAA 
CTGGATTTTG ATGAATGTCA CTCTAACCCT TGTCGGAATG GAGCCACCTG TGTGGACGGT 
CTGAATACAT TTAGATGCCT CTGCCTTCCG AGTTATGTCG GTGCACTCTG CGAACAAGAC 
ACTGAGACAT GCGACTATGG CTGGCACAAA TTCCAAGGGC AATGCTACAA GTACTTTGCT 
CATCGCCGTA CATGGGATGC TGCTGAAAGG GAGTGTCGCC TGCAGGGTGC CCACCTCACA 
AGCATCCTTT CTCATGAGGA ACAAATGTTT GTGAATCGTG TGGGCCATGA TTACCAGTGG 
ATTGGCCTCA ATGACAAGAT GTTTGAACAT GACTTCCGCT GGACTGACGG CAGCGCACTG 
CAATATGAGA ACTGGAGACC CAACCAGCCA GACAGCTTCT TTTCTGCTGG AGAAGACTGC 
GTTGTGATCA TTTGGCATGA GAATGGCCAG TGGAATGACG TCCCCTGCAA CTACCACCTC 
ACCTACACCT GCAAGAAGGG AACAGTTGCT TGCGGCCAAC CCCCTGTTGT AGAAAATGCC 
AAGACCTTTG GAAAGATGAA ACCACGTTAT GAAATCAACT CCTTGATTAG ATACCACTGC 
AAAGATGGTT TCATTCAGCG TCACCTTCCA ACTATCCGGT GCCTAGGAAA TGGGAGATGG 
GCAATGCCTA AAATAACCTG CATGAACCCA TCTGCATACC AAAGGACTTA TTCTAAGAAA 
TACTTAAAAA ATTCCTCATC AGTCAAGGAC AATTCTATAA ATACGTCAAA ACATGAGCAT 
CGCTGGAGCC GGAGGTGGCA GGAAACGAGG CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vcan ( NM_001170558.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001164029.1 Rattus norvegicus versican (Vcan), transcript variant 1, mRNA.

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CloneID	ORa39880
Clone ID Related Accession (Same CDS sequence)	NM_001170558.1
Accession Version	NM_001170558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576857600000
Organism	Rattus norvegicus(Norway rat)
Product	versican core protein isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF072892.1, AABR07007554.1, AF062402.1 and AY007691.1. On or before May 29, 2010 this sequence version replaced XM_215451.6, XM_001058357.2, XM_001058160.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also referred to as V0). Sequence Note: This RefSeq record was created from transcript and genomic sequence data from several strains because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01906348 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGATAA ATATGAACGG CATCCTATGG ATGTGCTCAA CCTTACTGTT AACGCATGCA 
CTGCATAAAG CCAAAATGGA AGAAAACCCA CCTGTTAAAG GCTCTCTGTC TGGAAAAGTG 
ATCCTACCTT GTCATTTTTC AACCTTGCCC ACCTTACCAC CCGATTACAA CACGAGTGAA 
TTTCTCAGAA TCAAATGGTC TAAAATAGAA GTGGACAAAA ATGGAAAAGA CATAAAGGAG 
ACTACTGTCC TGGTGGCCCA AGACGGGAAC ATCAAGATTG GTCAGGACTA CAAGGGGCGG 
GTATCAGTGC CTACGCATCC CGATGACGTA GGCGATGCCT CTCTCACCAT GGTCAAACTC 
CGTGCTAGTG ACGCAGGTGT CTACCGCTGT GATGTCATGT ATGGCATTGA AGACACTCAG 
AACACGATGT CGCTGGCCGT GGACGGTGTC GTGTTTCACT ACAGGGCAGC GACCAGCAGA 
TACACTCTGA ACTTCGAGTC TGCTCAACAG GCTTGTTTGG ACATCGGGGC GGTCATAGCA 
ACCCCAGAGC AGCTGTTCGC TGCCTATGAG GATGGATTTG AGCAGTGTGA TGCAGGATGG 
CTGTCTGACC AAACTGTCAG ATATCCCATA CGGGCTCCCC GAGAGGGCTG TTATGGAGAC 
ATGATGGGGA AGGAAGGGGT CCGGACCTAT GGATTCCGCT CTCCCCAGGA AACCTATGAT 
GTGTATTGCT ATGTGGATCA TCTGGACGGC GATGTGTTCC ACATCACTGC TCCCAGTAAA 
TTCACCTTCG AGGAGGCCGA AGCAGAGTGT GCAAACCGGG ATGCCAGGCT GGCGACTGTT 
GGGGAACTTC ACGCAGCTTG GAGGAACGGC TTTGACCAGT GCGATTACGG CTGGCTGTCG 
GATGCCAGCG TGCGGCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACATGCT TCCCTCTCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACCTAAACAG AATATATCAG AGGCAACCAC CATCGAAACG 
AATACCCTCG CAGAAACTGC ATCACCCAGT TTATCCAAAG AACCACACAT GGTACCCGAG 
AGAGCTACAC CAGTCATTCC ATTAGTCACC GAATTACCTA TTTTTACCAC ACACTTCCCT 
CCTGCAGGAA ATATAGTCAA TTCTGAACAA AAATCTGTAA TCTACTCTCA GGCTATCACA 
GACAGAGTAG CCACTGAATC ACCCACAGCT GCTGGGAGTC CCATTAACTC TTGGGATGCG 
GATGACTACT TGCCTTCTGG GTCAGGACCA CTTGGAAAGC CAGACATATC TGAAATTAAG 
GAGGAAGGAC TTCAAAGTAC AACTGTCACC TCCCAACATG CTACTGCCTC ACACCATGGA 
ATTACAGAGG ATACGCAAAC ACATGAATCA GTTACACAGA TTGAACAAAT AGAAGTGGGC 
CCCTTGGTAA CATCCATGGA AATCACAAAT CACATTTCTT TCAAGGAATT TCTAGAAACC 
AAAACACCAT TGGAATCCAC AGAAGTGACC CTGGAACAGC ATCAGACCGA CATGCCAACA 
GTTATCACCA GTCCTGAATT GGCAACCACA AGTCACTATG GAGTCACCTT GAGAGAAGAT 
GATAGAGAAG ATATAGCACT GACAGTAAGA TCTGGTCAGA GTACCCGGGT CTTTAGCCAA 
ATACCAGAAG TCATCACGGT ATCAAAGACA TCAGAAGACA CTACCTACTC ACAGCTAGGG 
GACTTAGAAT TCGTTTCAAC AGCAACAATA ACCATGCTTG GCACAGATGG ATCACTGACA 
GATGAAGGGA AAGAACCCCA AACTGATGAT AAGACGGCCG AAGACGAGTT TGGTCAATCA 
CAACCTACAA TCCCCTTCCC ATCACAGCAT CTTACAGAAG TAGAATCACT TCCATATTCT 
GGTGACACAA TATCAGTAGA GAGAATATCC ACAGTTAGTT ATCCATCATT ACAAACAGAT 
GTGACACAAG GAAGAGAGAG AACAGAAGTA CCAAGGCCAG GGTTAAAAAA AGATCCTTAT 
GCAGTTGATG AGATACAAGA AAAGATCACC AAAGATCCAT TTATAGGTAC AATAGAAGAA 
GGCTTCTCTG GAATGTCTCT CTCTACATCT TCCTCAGAGA CTTCAGCGGA AAGGACGGAA 
TCTGTTTCCC CAGCATTGAC AATTGAGAAG TTAACTGTGA AGCCAGCCGT AGCAAGCGAT 
GTGGACGAAA TGACAATCCT GACTAGATTA GAAACAGATG TGCCAACGTC TGACGAAGAT 
GTTACAAGTG CTCATTTGAC CCATAGTACT TTAAATGTGG AAGTGGTCAC AGTATTAAAA 
TGGCCAGGGA ATGAAGACAA TTCAACTTCA AAGCCTTTAC CCCCAACAGA ACGTGCAGGC 
TTTACAAAAT CGCCTCCTGT CTCACTTAGC ACTGTAGGAA TAGTTGGAAA AGATAAAGAA 
ACCCCAAGTT TCACAGATGG GGGAGACGAA TACACTCTTT CCCTAGATGG TACTCCCAAG 
CCATTAGAAA AGTTTTCTGA GGAAGATTTA ACATCTGGAG AATTTGCAGT TACAATTCCT 
ACATCTATAT CAATTGACAG TGCAGAGAAG TCAACTTTGG GAGAGCCTAC AACTGGAGAC 
AGGGTTCTGT CCACCACAAG CACAAAAGAT CTGGTCATCA ATGCAACTGT GGAAGGAAGT 
GCTTTGGATG AAGATATGGA TGCCTCCAAG CCACTGTTTA CTGCCACCCC ATTTGTCCAC 
ACCTCCGATG TGGAAGAATC GGCATTTGTT AATTATAGTA GCACCCAGCA GCCCACTACT 
TATGTAGACA TATCCCACAC TAGTCCTCTT TCTATAATTC CCAAGACAGA ATGGAGTGTA 
TCGGAAACTT CTGTTCCACT GGAAGATGAG GTTCTAGGCA AATCTGATCA AGATACCCTT 
GAACAGACAC ATCTAGAAGC AACCATGTCT CCTGAAGCTT TGAGTACAAT AGAAGTCACA 
CAGGGAGAAA CCCAGGAAGA ACCACAAACA CCAGGAATCC CATTTCCTGC TCTCAGTTCT 
ACTGCAGTTA TGACCAAGGA GACAACAGCA TTTGAAGAAG AGGGGGAGGG GTCAACATAT 
ACCCTCTCTG AAGACAGATT GATGACAGAT TCTGAAATAG TCCCAAGTTT AGAAACAACT 
CCAGTTGGTA CATCTTATCC AGGTGGTGCA ATGACACAGC AGGAAGTAGA AATGGATACA 
ATGGTAACAC AGATGTCAAG CATTAGGCCA ACAGTAGTTT TAAGTACTGA GCCAGAAGTA 
AGTTATGAAG CAGAAGGTAG TAGTCCAATG GAATTTGCAT CGACTTTGAA GCCCTTTGGG 
ACTCAAGTCA CACAGCTTGT GGAAGAAACG ACTGAGGAAG GGAAAAAGAC ACCCTTGGAT 
TATACAGATT TAGGTTCAGG GTTATTTGAA CAACCGAGAG TTACTGAGCT CCCAGATTTT 
TCAATGACTC CAAGTGATAT CAGTGTCTTC ACTGCCATCG ACAGCCTTCA CAGAACCACA 
CCCTTGAGGC CACCTTCGCC ATTCACTGAG GAACCACACA TCTTTGAGAA GGAGCCCAGT 
GAGAAAACAA CAGGTGACAT AATACTTCCT AGAGAATCTG TAACACAGCA CCCTCTGACA 
ACTCTCATGG ATATCATAGC CAAGAAAACA GAATCTGATA TTGATCACGA GTATCACATG 
ACCTCAAAGC CTCCTGTAAT GCAGCCAACA AGACCATCAG TTGTGGAAAG AAAAACAACT 
TCCAAACCTC AAGAGTTGTC TACTTCACCA CCCCCGGCAG GGACCAAATT CCACCCTGAC 
ATAAATGTTT ATATTATTGA GGTCAGAGAA AACAAGACAG GTCGAATGAG TGATATGGTT 
GTAAATGGTC ATCCAATAGA TTCAGAATCT AAAGAAGAGG AACCTTGCAG TGAAGAAACA 
GATCCACTGC ATGATCTGTT TGCTGAAATT TTACCCGAGT TACCAGATTC CTTCGAAATA 
GACATATATC ACAGTGAGGA AGATGAAGAT GGAGAGGAGG ACTGTGTAAA TGCAACTGAT 
GTAACAACCA CTCCGTCAGT GCAGTACATC ACTGGGAAGC CACATGTCAC TACAGTGCCC 
AAAAACCCAG AAGCTGCAGA AGCGAGGCGT GGCCTGTATG AAAGTGTTGC GCCTTCTCAG 
AATTTCTCAA ATACTTCCGC AACTGACACC CATCAGTTTA TACCGGCCGA AACAGAATTG 
TCAACTACCA TGCAATTTAC CAAATCCAAA GAAGCCACTG AATTGTTAGA AATCACATGG 
AAACCCGAGA CCTACCCTGA AACACCAGAA CACTTTTCAA GTGGTGAGCC TGATGTTTTC 
CCTACACTGC CATCCCATGA TGGTAAAACC ACCAAATGGT CAGAGTTCAT CACAGAGAGC 
AATCCAAACA CAGAAAATCC AGAACACAAA CAACCTAAGC CTATACCTCT GTTTCCTGAA 
GAGTTTTCAG GAGAGGGTGC CATTGACCAA GCATCCCAGC AAACAATCTT TTCCAGGGCT 
ACAGAAGTAG CACTTGGTAA AGAGACAGAC CAAAGTCCTA CCATATCTAC CTCTAGCATA 
CGTTCAGGTT CTGTGTCAGT ACATGCCTTG GAGGAAGATC CAATTGCTCT AACAGGAATC 
TCACAGACTG ATGAATCCAT GTCTACTGTG GAAAGCTGGG TAGAGATGAC CCCTAGCCAA 
ACTGTAGAGT TCTCTGGGAG TTCTTCAGCT CCTACTATTG AAGGGTCTGG GGAAGTAGAA 
GAATATACAA ATAAAATTTT CAACACGGTA ACTGATTTGC CACAGAGAGA GCCTACAGAT 
ACGCTCATCC CCTTGGATAT GAGCAACATC ATGATCACAG ACCACCACAT TTATACTCCT 
GCAACCACCG CTCCTTTGGA TTCACAGCTA CCTTCTACAG ATGCACGGCC TACACAATTT 
GGAATTCAAA CCACCACCTC TGAGTGGGTT TCCAGTACAT CTTTTGAAGG AAGGAAAACA 
GAGGAAGACA AAGAAAGAGA TACAAATGCA GCCCACACAG GGGAAGTCCA ACCAGCGACA 
GAGAGGTCAG ATCGCTTACT CTTAACTTCA GAATTAGAAA GTTCAAATGT GGCTGCATCT 
AGTCCTTTGG ACACCTGGGA AGGTTTTGTG CCAGAGACAA CATCAACAGT GTCCGAAAAG 
GAAATGGCAA ACACAACTCC TGTTTTTACA GAAACAAGTG ATGTAGCCAA TTTGGAGACA 
CAGTCTTTTG AGCATAGTAG TAGCAGTCAG CCCAGGGTAC AGGAAGAGCT GACCACTCTC 
TCAGGAAAGC CCCCCTTGAT TTTTATGGAC CTGGGTTCAG GAGATGCTAG TACTGATATG 
GAATTCATCA CTGCTTCTTC ATTTACATTA GATTTGGAGT CCGATACCAA AGTCAAAAAA 
GAATTACCCA GTACTCTGTC TCCCAGTGTG GAGACTTCAT CCTCCTCTGA GCCAATAGGA 
TTAGCTCCGA GTACTGTATT GGACATAGAA ATTGTGGAAG TTATGAATCA GACATCCAAG 
AAGACACTGA TTTCTGAACT ATCAGGAAAG CCAACCTCCC AAGCAGAAGT AAGGGATTTA 
TATCCAGGTC TGGGAGAAGA TTTTAGTGGT GACTCCAGTG AATATCCAAC AGTGTCTTCT 
ACAACAATGA AAGAGGAAAC AGTGGGGATG GGAGGCTCTG AGAATGAACG AGTTAAGGAT 
ACACAGACTT TATCATCTAT ACCACCTACC TCAGACAACA TCAACCCAGT GCCTGACTCA 
AAAGGATTCG GCAGTACTGT GGCCAGCACC ACAGCCTTCC CTTGGGAAGA GTTCATGACC 
TCAGCTGAGG GCTCAGGTGA GGAGCTGTCC TCAGTCAGGA GCTCTGTTAG TCTAGTGCTT 
CCACTAGGTG TGGACATTCT TCCTACTACA GAGTCTCCAT ATTTTGATCA AGAGTTTGAA 
GAAGCGGCTG CAGTGACAGA AGCAGGTAAA CAATCTGCTT TGCCAATAGC AGTGAGTGGA 
AATACTGTAG ATCTGACAGA AAACAGGGAC ATAGAGGTAA ACAGCACAAT GTCAGTAGAC 
CTTCCCCAGA CAATGGAGCC AGCCAAATTA TGGTCTAAAC CAGAAGTCAA TCCTGAAAAA 
CAAGAGATTG GAAGCGAAAC AGTGACACAG GATAAAGCTC AGGGACAAAA GTCTTTTGAA 
TCGCTCCACA GTTCTCTTGC ACCAGAACAA ACAACTTTGG AAAGCCAATC ACTTATTGAA 
ACTGAAGTCC AAACTTCATA TTACTCTATG TTAACAACAA TGAAAACATA CAATACTAAT 
GAGGAAGTGG AGGAGGAAGG CACGTCCATA GCTCATATGT CTACTCCAGG CCCTGGAATC 
AAAGGCTTGG AATCATATCC TACCCATCCT GAAGCTACTG GGAAGTCATA CTCTTTCTCA 
GCTTCTGCCT TAGTGACTGA ATCTGGACCA GCTAGAAGTG TAGTCATGGA TTCCTCAACT 
CAAGAGGAAG AAAGTATAAA ACTCTTCCAA AAGGACATGA TACTAACACA TAAAGAGTCA 
AATTCTGATC TCTCGTTCTC TGGCCTGGGA TCAGGAGAGG CTTTGCCTCC TCTCCCTACA 
ACATCAGTGA GTTTGACTGA CATGGGAAAG ATCAATAGCA CATTGTATCC TGAGACATCT 
CACATGGAAA GCTTAGGAAC AAGCATCTTA GGAGATAACC ATGAAAGAAT GAAAAATGTG 
TCAAATGAAG TTAGAACCCT TATTTCCGAA ACAGGCAGCA TTTCTCAAGA CAGTACGGAG 
GCACCCAATA CAACTTTGAG TGATACCAGA ACAGAAGAAT CTACAACTTC ACCTCTTCCT 
TTCATGAAGC TCATGGACAC GGAACATTCT CCAAAGCAGA CCCTCAGGTG GGAAGAGGAA 
ATCCAGACCC ATAGGCCACA AACTATGACT GGACAGATGA CCAATGACAA TTCTTCAGTG 
TCAGAAGCTG AAGCAGCAGC AACCTCTGCC CCTGCTTTCC TGCCTGAAAC CTACAGTGTT 
GAAATGACCA AAGCCTTTGC TACATCACCG TCACAAACAT CTGACTTGTT TGATGCAAAT 
TCTGGAGAAG GATCTGGAGA AGTGGATGGT CTTGACTTAG TGTACACTTC TAGAACTACT 
CAGGCAAGCA GTCAAGGAGA CAGCATGTTT GCCTCTCATG GATTCATTGA AAAGCATCCA 
GAGGTGTCAA GGACCGAGAC TGGAGCTACT GATGGATCTC CAACAGCTTC AGCGATGTTC 
CTGCATCAGT CAGAGTACAA TGAGAGTTCC CTGTATCCAA CCAGCACACT GCCAAGCACA 
GTGACATATG AGAGTCCCTC AGAAGGTATT GCAGATGGTT TGCAAGACCA TATCAGATTC 
GAAGTTTCTA CCTTGAAACC TAGCAGAAGG AAAGCCACTG AGAGCGTTAT TATAGATCTA 
GACAAAGAGG ATAGTAAGGA CTTAGGGTTG GCAATTACTG AGAGTGCTAT TGTTGAAATT 
CTCCCTGAGC TGACATCAGA TAGAAATATT ATTATAGACA TTGATCACAC TAAACCTGTA 
TATGAGTACA TCCCTGGAAT ACAAACAGAC CTAGATTCAG ACATACCATT GGGATCACAT 
GGCAGTAGTG AAGAAAGCCT TGAAGTTCAA GAGAAGTATG AAGCAACTAT TAACCTGTCT 
CCAACTGAGG AAGCATTTGA CGGCTCTGGT GATGCACTTC CTGCTGGTCA CACTCAAGCG 
ATATACAATG AGTCTGTGAC TCCCAGTGAT GGAAAGCAGC CAGAGGACAT AAGCTTTAGT 
TTTGCAACTG GAATCCCAGT TTCTAGCACA GAGACAGAAT TAAATACTTT CTTCCCTACA 
GTGTCAACCC TGCACATTCC TAGCAAACTA ACCACCGCTA GTCCAGAGAT TGATAAACCC 
AACATTGAAG CAATATCACT GGATGATATA TTTGAATCAA GCACCTTGTC TGATGGCCAA 
GCTATTGCCG ATCAGAGTGA AGTAATATCA ACACTAGGCC ATTTGGAAAA GACACAGGAA 
GAGTATGAAG AAAAGAAATA TGGAGGTCCT TCTTTTCAGC CAGAATTCTT TTCAGGAGTG 
GGGGAAGTAT TCACAGATGC CCCTGCTTAT GTAAGTATTG GTAGGACATA CTCTGTAGCT 
CAGCCTTTAA CAGAATTCCC CAATGTGGTA GGACAATCTG ACTCCACACA TTACACTGAA 
GCAACATCTG CAGTTTCCTC TGTGACAGAG CTGTCTCCTC AGACACCATC TTCTCCCTCT 
CCTGTCTACA TAGACAGTGG AGTCTCTGAA TTCACAGAGG TCCCCCACAA AAGTGCTCAG 
CCAGCTCCTA CTGCCGCTTC ATCACAAAAG TTGATAGAGG GTTCTTTTAA GAAAGTGCGT 
GCTAATATTG AAGCTACCAT CAAGTCGCTG GGTGAAAATG ATCACGGAAC AGAGTCTCCA 
TCCATGTCTC CTAGCCCTGC TTTAGACATT TCAGAAGACG ACAGTAAACC TAAGTTACTA 
GAAGACCTGG AAACTTCTCC CACAAAAACC GAGACTTCCC AAGATTCCCC AAACAAAGCA 
AATGATCAAA TTCCAGGAAA AACAGCTGGA ATCCTTGCTG GTATCAAAAC AACAGAGAGT 
GGACCAGTCG TTACAGCTGC AGATGACATG GAGCTAGGAG ATGCCACACA GCGGCCACAT 
TCTGCTTCTG CTCCTGCAGC TTTCAGGGTT GAGACAAGTA TGGTACCTCA GCCCATCCCA 
CAGGAGCCTG AGAGGCCAAC CTTTCCTTCC CTGGAAATTA ACCATGAAAC ACACACATCA 
TTATTTGAAG AATCTATATT GGCAACATCT GAGAAACAAG TGTCCCAAAG AATTCTTGAT 
TACAGTAACC AGGCAACAGT CAGTACTCTG GATCTAAATA CTGAACACTC AATACCACCA 
TTTTCCATTC TGGACAATTC TAACGAAACT GCTTTCCTGA TTGGCATTAG TGAAGAGACC 
GTGGAAGGCA CAGCAGTTTA TCTACCAGGA CCTGATCTCT GCAAAACAAA CCCATGCCTC 
AATGGAGGCA CCTGCTATCC TACTGAGACT TCCTATGTGT GCACCTGTGC ACCTGGCTAC 
AGTGGAGACC AGTGTGAACT GGATTTTGAT GAATGTCACT CTAACCCTTG TCGGAATGGA 
GCCACCTGTG TGGACGGTCT GAATACATTT AGATGCCTCT GCCTTCCGAG TTATGTCGGT 
GCACTCTGCG AACAAGACAC TGAGACATGC GACTATGGCT GGCACAAATT CCAAGGGCAA 
TGCTACAAGT ACTTTGCTCA TCGCCGTACA TGGGATGCTG CTGAAAGGGA GTGTCGCCTG 
CAGGGTGCCC ACCTCACAAG CATCCTTTCT CATGAGGAAC AAATGTTTGT GAATCGTGTG 
GGCCATGATT ACCAGTGGAT TGGCCTCAAT GACAAGATGT TTGAACATGA CTTCCGCTGG 
ACTGACGGCA GCGCACTGCA ATATGAGAAC TGGAGACCCA ACCAGCCAGA CAGCTTCTTT 
TCTGCTGGAG AAGACTGCGT TGTGATCATT TGGCATGAGA ATGGCCAGTG GAATGACGTC 
CCCTGCAACT ACCACCTCAC CTACACCTGC AAGAAGGGAA CAGTTGCTTG CGGCCAACCC 
CCTGTTGTAG AAAATGCCAA GACCTTTGGA AAGATGAAAC CACGTTATGA AATCAACTCC 
TTGATTAGAT ACCACTGCAA AGATGGTTTC ATTCAGCGTC ACCTTCCAAC TATCCGGTGC 
CTAGGAAATG GGAGATGGGC AATGCCTAAA ATAACCTGCA TGAACCCATC TGCATACCAA 
AGGACTTATT CTAAGAAATA CTTAAAAAAT TCCTCATCAG TCAAGGACAA TTCTATAAAT 
ACGTCAAAAC ATGAGCATCG CTGGAGCCGG AGGTGGCAGG AAACGAGGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164029.1
CDS	281..10354
Translation	MLINMNGILWMCSTLLLTHALHKAKMEENPPVKGSLSGKVILPCHFSTLPTLPPDYNTSEFLRIKWSKIEVDKNGKDIKETTVLVAQDGNIKIGQDYKGRVSVPTHPDDVGDASLTMVKLRASDAGVYRCDVMYGIEDTQNTMSLAVDGVVFHYRAATSRYTLNFESAQQACLDIGAVIATPEQLFAAYEDGFEQCDAGWLSDQTVRYPIRAPREGCYGDMMGKEGVRTYGFRSPQETYDVYCYVDHLDGDVFHITAPSKFTFEEAEAECANRDARLATVGELHAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTCFPLPDSRFDAYCFKPKQNISEATTIETNTLAETASPSLSKEPHMVPERATPVIPLVTELPIFTTHFPPAGNIVNSEQKSVIYSQAITDRVATESPTAAGSPINSWDADDYLPSGSGPLGKPDISEIKEEGLQSTTVTSQHATASHHGITEDTQTHESVTQIEQIEVGPLVTSMEITNHISFKEFLETKTPLESTEVTLEQHQTDMPTVITSPELATTSHYGVTLREDDREDIALTVRSGQSTRVFSQIPEVITVSKTSEDTTYSQLGDLEFVSTATITMLGTDGSLTDEGKEPQTDDKTAEDEFGQSQPTIPFPSQHLTEVESLPYSGDTISVERISTVSYPSLQTDVTQGRERTEVPRPGLKKDPYAVDEIQEKITKDPFIGTIEEGFSGMSLSTSSSETSAERTESVSPALTIEKLTVKPAVASDVDEMTILTRLETDVPTSDEDVTSAHLTHSTLNVEVVTVLKWPGNEDNSTSKPLPPTERAGFTKSPPVSLSTVGIVGKDKETPSFTDGGDEYTLSLDGTPKPLEKFSEEDLTSGEFAVTIPTSISIDSAEKSTLGEPTTGDRVLSTTSTKDLVINATVEGSALDEDMDASKPLFTATPFVHTSDVEESAFVNYSSTQQPTTYVDISHTSPLSIIPKTEWSVSETSVPLEDEVLGKSDQDTLEQTHLEATMSPEALSTIEVTQGETQEEPQTPGIPFPALSSTAVMTKETTAFEEEGEGSTYTLSEDRLMTDSEIVPSLETTPVGTSYPGGAMTQQEVEMDTMVTQMSSIRPTVVLSTEPEVSYEAEGSSPMEFASTLKPFGTQVTQLVEETTEEGKKTPLDYTDLGSGLFEQPRVTELPDFSMTPSDISVFTAIDSLHRTTPLRPPSPFTEEPHIFEKEPSEKTTGDIILPRESVTQHPLTTLMDIIAKKTESDIDHEYHMTSKPPVMQPTRPSVVERKTTSKPQELSTSPPPAGTKFHPDINVYIIEVRENKTGRMSDMVVNGHPIDSESKEEEPCSEETDPLHDLFAEILPELPDSFEIDIYHSEEDEDGEEDCVNATDVTTTPSVQYITGKPHVTTVPKNPEAAEARRGLYESVAPSQNFSNTSATDTHQFIPAETELSTTMQFTKSKEATELLEITWKPETYPETPEHFSSGEPDVFPTLPSHDGKTTKWSEFITESNPNTENPEHKQPKPIPLFPEEFSGEGAIDQASQQTIFSRATEVALGKETDQSPTISTSSIRSGSVSVHALEEDPIALTGISQTDESMSTVESWVEMTPSQTVEFSGSSSAPTIEGSGEVEEYTNKIFNTVTDLPQREPTDTLIPLDMSNIMITDHHIYTPATTAPLDSQLPSTDARPTQFGIQTTTSEWVSSTSFEGRKTEEDKERDTNAAHTGEVQPATERSDRLLLTSELESSNVAASSPLDTWEGFVPETTSTVSEKEMANTTPVFTETSDVANLETQSFEHSSSSQPRVQEELTTLSGKPPLIFMDLGSGDASTDMEFITASSFTLDLESDTKVKKELPSTLSPSVETSSSSEPIGLAPSTVLDIEIVEVMNQTSKKTLISELSGKPTSQAEVRDLYPGLGEDFSGDSSEYPTVSSTTMKEETVGMGGSENERVKDTQTLSSIPPTSDNINPVPDSKGFGSTVASTTAFPWEEFMTSAEGSGEELSSVRSSVSLVLPLGVDILPTTESPYFDQEFEEAAAVTEAGKQSALPIAVSGNTVDLTENRDIEVNSTMSVDLPQTMEPAKLWSKPEVNPEKQEIGSETVTQDKAQGQKSFESLHSSLAPEQTTLESQSLIETEVQTSYYSMLTTMKTYNTNEEVEEEGTSIAHMSTPGPGIKGLESYPTHPEATGKSYSFSASALVTESGPARSVVMDSSTQEEESIKLFQKDMILTHKESNSDLSFSGLGSGEALPPLPTTSVSLTDMGKINSTLYPETSHMESLGTSILGDNHERMKNVSNEVRTLISETGSISQDSTEAPNTTLSDTRTEESTTSPLPFMKLMDTEHSPKQTLRWEEEIQTHRPQTMTGQMTNDNSSVSEAEAAATSAPAFLPETYSVEMTKAFATSPSQTSDLFDANSGEGSGEVDGLDLVYTSRTTQASSQGDSMFASHGFIEKHPEVSRTETGATDGSPTASAMFLHQSEYNESSLYPTSTLPSTVTYESPSEGIADGLQDHIRFEVSTLKPSRRKATESVIIDLDKEDSKDLGLAITESAIVEILPELTSDRNIIIDIDHTKPVYEYIPGIQTDLDSDIPLGSHGSSEESLEVQEKYEATINLSPTEEAFDGSGDALPAGHTQAIYNESVTPSDGKQPEDISFSFATGIPVSSTETELNTFFPTVSTLHIPSKLTTASPEIDKPNIEAISLDDIFESSTLSDGQAIADQSEVISTLGHLEKTQEEYEEKKYGGPSFQPEFFSGVGEVFTDAPAYVSIGRTYSVAQPLTEFPNVVGQSDSTHYTEATSAVSSVTELSPQTPSSPSPVYIDSGVSEFTEVPHKSAQPAPTAASSQKLIEGSFKKVRANIEATIKSLGENDHGTESPSMSPSPALDISEDDSKPKLLEDLETSPTKTETSQDSPNKANDQIPGKTAGILAGIKTTESGPVVTAADDMELGDATQRPHSASAPAAFRVETSMVPQPIPQEPERPTFPSLEINHETHTSLFEESILATSEKQVSQRILDYSNQATVSTLDLNTEHSIPPFSILDNSNETAFLIGISEETVEGTAVYLPGPDLCKTNPCLNGGTCYPTETSYVCTCAPGYSGDQCELDFDECHSNPCRNGATCVDGLNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSALQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAMPKITCMNPSAYQRTYSKKYLKNSSSVKDNSINTSKHEHRWSRRWQETRR

Target ORF information:

RefSeq Version	NM_001170558.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus versican (Vcan), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170558.1

ORF Insert Sequence:

ATGTTGATAA ATATGAACGG CATCCTATGG ATGTGCTCAA CCTTACTGTT AACGCATGCA 
CTGCATAAAG CCAAAATGGA AGAAAACCCA CCTGTTAAAG GCTCTCTGTC TGGAAAAGTG 
ATCCTACCTT GTCATTTTTC AACCTTGCCC ACCTTACCAC CCGATTACAA CACGAGTGAA 
TTTCTCAGAA TCAAATGGTC TAAAATAGAA GTGGACAAAA ATGGAAAAGA CATAAAGGAG 
ACTACTGTCC TGGTGGCCCA AGACGGGAAC ATCAAGATTG GTCAGGACTA CAAGGGGCGG 
GTATCAGTGC CTACGCATCC CGATGACGTA GGCGATGCCT CTCTCACCAT GGTCAAACTC 
CGTGCTAGTG ACGCAGGTGT CTACCGCTGT GATGTCATGT ATGGCATTGA AGACACTCAG 
AACACGATGT CGCTGGCCGT GGACGGTGTC GTGTTTCACT ACAGGGCAGC GACCAGCAGA 
TACACTCTGA ACTTCGAGTC TGCTCAACAG GCTTGTTTGG ACATCGGGGC GGTCATAGCA 
ACCCCAGAGC AGCTGTTCGC TGCCTATGAG GATGGATTTG AGCAGTGTGA TGCAGGATGG 
CTGTCTGACC AAACTGTCAG ATATCCCATA CGGGCTCCCC GAGAGGGCTG TTATGGAGAC 
ATGATGGGGA AGGAAGGGGT CCGGACCTAT GGATTCCGCT CTCCCCAGGA AACCTATGAT 
GTGTATTGCT ATGTGGATCA TCTGGACGGC GATGTGTTCC ACATCACTGC TCCCAGTAAA 
TTCACCTTCG AGGAGGCCGA AGCAGAGTGT GCAAACCGGG ATGCCAGGCT GGCGACTGTT 
GGGGAACTTC ACGCAGCTTG GAGGAACGGC TTTGACCAGT GCGATTACGG CTGGCTGTCG 
GATGCCAGCG TGCGGCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACATGCT TCCCTCTCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACCTAAACAG AATATATCAG AGGCAACCAC CATCGAAACG 
AATACCCTCG CAGAAACTGC ATCACCCAGT TTATCCAAAG AACCACACAT GGTACCCGAG 
AGAGCTACAC CAGTCATTCC ATTAGTCACC GAATTACCTA TTTTTACCAC ACACTTCCCT 
CCTGCAGGAA ATATAGTCAA TTCTGAACAA AAATCTGTAA TCTACTCTCA GGCTATCACA 
GACAGAGTAG CCACTGAATC ACCCACAGCT GCTGGGAGTC CCATTAACTC TTGGGATGCG 
GATGACTACT TGCCTTCTGG GTCAGGACCA CTTGGAAAGC CAGACATATC TGAAATTAAG 
GAGGAAGGAC TTCAAAGTAC AACTGTCACC TCCCAACATG CTACTGCCTC ACACCATGGA 
ATTACAGAGG ATACGCAAAC ACATGAATCA GTTACACAGA TTGAACAAAT AGAAGTGGGC 
CCCTTGGTAA CATCCATGGA AATCACAAAT CACATTTCTT TCAAGGAATT TCTAGAAACC 
AAAACACCAT TGGAATCCAC AGAAGTGACC CTGGAACAGC ATCAGACCGA CATGCCAACA 
GTTATCACCA GTCCTGAATT GGCAACCACA AGTCACTATG GAGTCACCTT GAGAGAAGAT 
GATAGAGAAG ATATAGCACT GACAGTAAGA TCTGGTCAGA GTACCCGGGT CTTTAGCCAA 
ATACCAGAAG TCATCACGGT ATCAAAGACA TCAGAAGACA CTACCTACTC ACAGCTAGGG 
GACTTAGAAT TCGTTTCAAC AGCAACAATA ACCATGCTTG GCACAGATGG ATCACTGACA 
GATGAAGGGA AAGAACCCCA AACTGATGAT AAGACGGCCG AAGACGAGTT TGGTCAATCA 
CAACCTACAA TCCCCTTCCC ATCACAGCAT CTTACAGAAG TAGAATCACT TCCATATTCT 
GGTGACACAA TATCAGTAGA GAGAATATCC ACAGTTAGTT ATCCATCATT ACAAACAGAT 
GTGACACAAG GAAGAGAGAG AACAGAAGTA CCAAGGCCAG GGTTAAAAAA AGATCCTTAT 
GCAGTTGATG AGATACAAGA AAAGATCACC AAAGATCCAT TTATAGGTAC AATAGAAGAA 
GGCTTCTCTG GAATGTCTCT CTCTACATCT TCCTCAGAGA CTTCAGCGGA AAGGACGGAA 
TCTGTTTCCC CAGCATTGAC AATTGAGAAG TTAACTGTGA AGCCAGCCGT AGCAAGCGAT 
GTGGACGAAA TGACAATCCT GACTAGATTA GAAACAGATG TGCCAACGTC TGACGAAGAT 
GTTACAAGTG CTCATTTGAC CCATAGTACT TTAAATGTGG AAGTGGTCAC AGTATTAAAA 
TGGCCAGGGA ATGAAGACAA TTCAACTTCA AAGCCTTTAC CCCCAACAGA ACGTGCAGGC 
TTTACAAAAT CGCCTCCTGT CTCACTTAGC ACTGTAGGAA TAGTTGGAAA AGATAAAGAA 
ACCCCAAGTT TCACAGATGG GGGAGACGAA TACACTCTTT CCCTAGATGG TACTCCCAAG 
CCATTAGAAA AGTTTTCTGA GGAAGATTTA ACATCTGGAG AATTTGCAGT TACAATTCCT 
ACATCTATAT CAATTGACAG TGCAGAGAAG TCAACTTTGG GAGAGCCTAC AACTGGAGAC 
AGGGTTCTGT CCACCACAAG CACAAAAGAT CTGGTCATCA ATGCAACTGT GGAAGGAAGT 
GCTTTGGATG AAGATATGGA TGCCTCCAAG CCACTGTTTA CTGCCACCCC ATTTGTCCAC 
ACCTCCGATG TGGAAGAATC GGCATTTGTT AATTATAGTA GCACCCAGCA GCCCACTACT 
TATGTAGACA TATCCCACAC TAGTCCTCTT TCTATAATTC CCAAGACAGA ATGGAGTGTA 
TCGGAAACTT CTGTTCCACT GGAAGATGAG GTTCTAGGCA AATCTGATCA AGATACCCTT 
GAACAGACAC ATCTAGAAGC AACCATGTCT CCTGAAGCTT TGAGTACAAT AGAAGTCACA 
CAGGGAGAAA CCCAGGAAGA ACCACAAACA CCAGGAATCC CATTTCCTGC TCTCAGTTCT 
ACTGCAGTTA TGACCAAGGA GACAACAGCA TTTGAAGAAG AGGGGGAGGG GTCAACATAT 
ACCCTCTCTG AAGACAGATT GATGACAGAT TCTGAAATAG TCCCAAGTTT AGAAACAACT 
CCAGTTGGTA CATCTTATCC AGGTGGTGCA ATGACACAGC AGGAAGTAGA AATGGATACA 
ATGGTAACAC AGATGTCAAG CATTAGGCCA ACAGTAGTTT TAAGTACTGA GCCAGAAGTA 
AGTTATGAAG CAGAAGGTAG TAGTCCAATG GAATTTGCAT CGACTTTGAA GCCCTTTGGG 
ACTCAAGTCA CACAGCTTGT GGAAGAAACG ACTGAGGAAG GGAAAAAGAC ACCCTTGGAT 
TATACAGATT TAGGTTCAGG GTTATTTGAA CAACCGAGAG TTACTGAGCT CCCAGATTTT 
TCAATGACTC CAAGTGATAT CAGTGTCTTC ACTGCCATCG ACAGCCTTCA CAGAACCACA 
CCCTTGAGGC CACCTTCGCC ATTCACTGAG GAACCACACA TCTTTGAGAA GGAGCCCAGT 
GAGAAAACAA CAGGTGACAT AATACTTCCT AGAGAATCTG TAACACAGCA CCCTCTGACA 
ACTCTCATGG ATATCATAGC CAAGAAAACA GAATCTGATA TTGATCACGA GTATCACATG 
ACCTCAAAGC CTCCTGTAAT GCAGCCAACA AGACCATCAG TTGTGGAAAG AAAAACAACT 
TCCAAACCTC AAGAGTTGTC TACTTCACCA CCCCCGGCAG GGACCAAATT CCACCCTGAC 
ATAAATGTTT ATATTATTGA GGTCAGAGAA AACAAGACAG GTCGAATGAG TGATATGGTT 
GTAAATGGTC ATCCAATAGA TTCAGAATCT AAAGAAGAGG AACCTTGCAG TGAAGAAACA 
GATCCACTGC ATGATCTGTT TGCTGAAATT TTACCCGAGT TACCAGATTC CTTCGAAATA 
GACATATATC ACAGTGAGGA AGATGAAGAT GGAGAGGAGG ACTGTGTAAA TGCAACTGAT 
GTAACAACCA CTCCGTCAGT GCAGTACATC ACTGGGAAGC CACATGTCAC TACAGTGCCC 
AAAAACCCAG AAGCTGCAGA AGCGAGGCGT GGCCTGTATG AAAGTGTTGC GCCTTCTCAG 
AATTTCTCAA ATACTTCCGC AACTGACACC CATCAGTTTA TACCGGCCGA AACAGAATTG 
TCAACTACCA TGCAATTTAC CAAATCCAAA GAAGCCACTG AATTGTTAGA AATCACATGG 
AAACCCGAGA CCTACCCTGA AACACCAGAA CACTTTTCAA GTGGTGAGCC TGATGTTTTC 
CCTACACTGC CATCCCATGA TGGTAAAACC ACCAAATGGT CAGAGTTCAT CACAGAGAGC 
AATCCAAACA CAGAAAATCC AGAACACAAA CAACCTAAGC CTATACCTCT GTTTCCTGAA 
GAGTTTTCAG GAGAGGGTGC CATTGACCAA GCATCCCAGC AAACAATCTT TTCCAGGGCT 
ACAGAAGTAG CACTTGGTAA AGAGACAGAC CAAAGTCCTA CCATATCTAC CTCTAGCATA 
CGTTCAGGTT CTGTGTCAGT ACATGCCTTG GAGGAAGATC CAATTGCTCT AACAGGAATC 
TCACAGACTG ATGAATCCAT GTCTACTGTG GAAAGCTGGG TAGAGATGAC CCCTAGCCAA 
ACTGTAGAGT TCTCTGGGAG TTCTTCAGCT CCTACTATTG AAGGGTCTGG GGAAGTAGAA 
GAATATACAA ATAAAATTTT CAACACGGTA ACTGATTTGC CACAGAGAGA GCCTACAGAT 
ACGCTCATCC CCTTGGATAT GAGCAACATC ATGATCACAG ACCACCACAT TTATACTCCT 
GCAACCACCG CTCCTTTGGA TTCACAGCTA CCTTCTACAG ATGCACGGCC TACACAATTT 
GGAATTCAAA CCACCACCTC TGAGTGGGTT TCCAGTACAT CTTTTGAAGG AAGGAAAACA 
GAGGAAGACA AAGAAAGAGA TACAAATGCA GCCCACACAG GGGAAGTCCA ACCAGCGACA 
GAGAGGTCAG ATCGCTTACT CTTAACTTCA GAATTAGAAA GTTCAAATGT GGCTGCATCT 
AGTCCTTTGG ACACCTGGGA AGGTTTTGTG CCAGAGACAA CATCAACAGT GTCCGAAAAG 
GAAATGGCAA ACACAACTCC TGTTTTTACA GAAACAAGTG ATGTAGCCAA TTTGGAGACA 
CAGTCTTTTG AGCATAGTAG TAGCAGTCAG CCCAGGGTAC AGGAAGAGCT GACCACTCTC 
TCAGGAAAGC CCCCCTTGAT TTTTATGGAC CTGGGTTCAG GAGATGCTAG TACTGATATG 
GAATTCATCA CTGCTTCTTC ATTTACATTA GATTTGGAGT CCGATACCAA AGTCAAAAAA 
GAATTACCCA GTACTCTGTC TCCCAGTGTG GAGACTTCAT CCTCCTCTGA GCCAATAGGA 
TTAGCTCCGA GTACTGTATT GGACATAGAA ATTGTGGAAG TTATGAATCA GACATCCAAG 
AAGACACTGA TTTCTGAACT ATCAGGAAAG CCAACCTCCC AAGCAGAAGT AAGGGATTTA 
TATCCAGGTC TGGGAGAAGA TTTTAGTGGT GACTCCAGTG AATATCCAAC AGTGTCTTCT 
ACAACAATGA AAGAGGAAAC AGTGGGGATG GGAGGCTCTG AGAATGAACG AGTTAAGGAT 
ACACAGACTT TATCATCTAT ACCACCTACC TCAGACAACA TCAACCCAGT GCCTGACTCA 
AAAGGATTCG GCAGTACTGT GGCCAGCACC ACAGCCTTCC CTTGGGAAGA GTTCATGACC 
TCAGCTGAGG GCTCAGGTGA GGAGCTGTCC TCAGTCAGGA GCTCTGTTAG TCTAGTGCTT 
CCACTAGGTG TGGACATTCT TCCTACTACA GAGTCTCCAT ATTTTGATCA AGAGTTTGAA 
GAAGCGGCTG CAGTGACAGA AGCAGGTAAA CAATCTGCTT TGCCAATAGC AGTGAGTGGA 
AATACTGTAG ATCTGACAGA AAACAGGGAC ATAGAGGTAA ACAGCACAAT GTCAGTAGAC 
CTTCCCCAGA CAATGGAGCC AGCCAAATTA TGGTCTAAAC CAGAAGTCAA TCCTGAAAAA 
CAAGAGATTG GAAGCGAAAC AGTGACACAG GATAAAGCTC AGGGACAAAA GTCTTTTGAA 
TCGCTCCACA GTTCTCTTGC ACCAGAACAA ACAACTTTGG AAAGCCAATC ACTTATTGAA 
ACTGAAGTCC AAACTTCATA TTACTCTATG TTAACAACAA TGAAAACATA CAATACTAAT 
GAGGAAGTGG AGGAGGAAGG CACGTCCATA GCTCATATGT CTACTCCAGG CCCTGGAATC 
AAAGGCTTGG AATCATATCC TACCCATCCT GAAGCTACTG GGAAGTCATA CTCTTTCTCA 
GCTTCTGCCT TAGTGACTGA ATCTGGACCA GCTAGAAGTG TAGTCATGGA TTCCTCAACT 
CAAGAGGAAG AAAGTATAAA ACTCTTCCAA AAGGACATGA TACTAACACA TAAAGAGTCA 
AATTCTGATC TCTCGTTCTC TGGCCTGGGA TCAGGAGAGG CTTTGCCTCC TCTCCCTACA 
ACATCAGTGA GTTTGACTGA CATGGGAAAG ATCAATAGCA CATTGTATCC TGAGACATCT 
CACATGGAAA GCTTAGGAAC AAGCATCTTA GGAGATAACC ATGAAAGAAT GAAAAATGTG 
TCAAATGAAG TTAGAACCCT TATTTCCGAA ACAGGCAGCA TTTCTCAAGA CAGTACGGAG 
GCACCCAATA CAACTTTGAG TGATACCAGA ACAGAAGAAT CTACAACTTC ACCTCTTCCT 
TTCATGAAGC TCATGGACAC GGAACATTCT CCAAAGCAGA CCCTCAGGTG GGAAGAGGAA 
ATCCAGACCC ATAGGCCACA AACTATGACT GGACAGATGA CCAATGACAA TTCTTCAGTG 
TCAGAAGCTG AAGCAGCAGC AACCTCTGCC CCTGCTTTCC TGCCTGAAAC CTACAGTGTT 
GAAATGACCA AAGCCTTTGC TACATCACCG TCACAAACAT CTGACTTGTT TGATGCAAAT 
TCTGGAGAAG GATCTGGAGA AGTGGATGGT CTTGACTTAG TGTACACTTC TAGAACTACT 
CAGGCAAGCA GTCAAGGAGA CAGCATGTTT GCCTCTCATG GATTCATTGA AAAGCATCCA 
GAGGTGTCAA GGACCGAGAC TGGAGCTACT GATGGATCTC CAACAGCTTC AGCGATGTTC 
CTGCATCAGT CAGAGTACAA TGAGAGTTCC CTGTATCCAA CCAGCACACT GCCAAGCACA 
GTGACATATG AGAGTCCCTC AGAAGGTATT GCAGATGGTT TGCAAGACCA TATCAGATTC 
GAAGTTTCTA CCTTGAAACC TAGCAGAAGG AAAGCCACTG AGAGCGTTAT TATAGATCTA 
GACAAAGAGG ATAGTAAGGA CTTAGGGTTG GCAATTACTG AGAGTGCTAT TGTTGAAATT 
CTCCCTGAGC TGACATCAGA TAGAAATATT ATTATAGACA TTGATCACAC TAAACCTGTA 
TATGAGTACA TCCCTGGAAT ACAAACAGAC CTAGATTCAG ACATACCATT GGGATCACAT 
GGCAGTAGTG AAGAAAGCCT TGAAGTTCAA GAGAAGTATG AAGCAACTAT TAACCTGTCT 
CCAACTGAGG AAGCATTTGA CGGCTCTGGT GATGCACTTC CTGCTGGTCA CACTCAAGCG 
ATATACAATG AGTCTGTGAC TCCCAGTGAT GGAAAGCAGC CAGAGGACAT AAGCTTTAGT 
TTTGCAACTG GAATCCCAGT TTCTAGCACA GAGACAGAAT TAAATACTTT CTTCCCTACA 
GTGTCAACCC TGCACATTCC TAGCAAACTA ACCACCGCTA GTCCAGAGAT TGATAAACCC 
AACATTGAAG CAATATCACT GGATGATATA TTTGAATCAA GCACCTTGTC TGATGGCCAA 
GCTATTGCCG ATCAGAGTGA AGTAATATCA ACACTAGGCC ATTTGGAAAA GACACAGGAA 
GAGTATGAAG AAAAGAAATA TGGAGGTCCT TCTTTTCAGC CAGAATTCTT TTCAGGAGTG 
GGGGAAGTAT TCACAGATGC CCCTGCTTAT GTAAGTATTG GTAGGACATA CTCTGTAGCT 
CAGCCTTTAA CAGAATTCCC CAATGTGGTA GGACAATCTG ACTCCACACA TTACACTGAA 
GCAACATCTG CAGTTTCCTC TGTGACAGAG CTGTCTCCTC AGACACCATC TTCTCCCTCT 
CCTGTCTACA TAGACAGTGG AGTCTCTGAA TTCACAGAGG TCCCCCACAA AAGTGCTCAG 
CCAGCTCCTA CTGCCGCTTC ATCACAAAAG TTGATAGAGG GTTCTTTTAA GAAAGTGCGT 
GCTAATATTG AAGCTACCAT CAAGTCGCTG GGTGAAAATG ATCACGGAAC AGAGTCTCCA 
TCCATGTCTC CTAGCCCTGC TTTAGACATT TCAGAAGACG ACAGTAAACC TAAGTTACTA 
GAAGACCTGG AAACTTCTCC CACAAAAACC GAGACTTCCC AAGATTCCCC AAACAAAGCA 
AATGATCAAA TTCCAGGAAA AACAGCTGGA ATCCTTGCTG GTATCAAAAC AACAGAGAGT 
GGACCAGTCG TTACAGCTGC AGATGACATG GAGCTAGGAG ATGCCACACA GCGGCCACAT 
TCTGCTTCTG CTCCTGCAGC TTTCAGGGTT GAGACAAGTA TGGTACCTCA GCCCATCCCA 
CAGGAGCCTG AGAGGCCAAC CTTTCCTTCC CTGGAAATTA ACCATGAAAC ACACACATCA 
TTATTTGAAG AATCTATATT GGCAACATCT GAGAAACAAG TGTCCCAAAG AATTCTTGAT 
TACAGTAACC AGGCAACAGT CAGTACTCTG GATCTAAATA CTGAACACTC AATACCACCA 
TTTTCCATTC TGGACAATTC TAACGAAACT GCTTTCCTGA TTGGCATTAG TGAAGAGACC 
GTGGAAGGCA CAGCAGTTTA TCTACCAGGA CCTGATCTCT GCAAAACAAA CCCATGCCTC 
AATGGAGGCA CCTGCTATCC TACTGAGACT TCCTATGTGT GCACCTGTGC ACCTGGCTAC 
AGTGGAGACC AGTGTGAACT GGATTTTGAT GAATGTCACT CTAACCCTTG TCGGAATGGA 
GCCACCTGTG TGGACGGTCT GAATACATTT AGATGCCTCT GCCTTCCGAG TTATGTCGGT 
GCACTCTGCG AACAAGACAC TGAGACATGC GACTATGGCT GGCACAAATT CCAAGGGCAA 
TGCTACAAGT ACTTTGCTCA TCGCCGTACA TGGGATGCTG CTGAAAGGGA GTGTCGCCTG 
CAGGGTGCCC ACCTCACAAG CATCCTTTCT CATGAGGAAC AAATGTTTGT GAATCGTGTG 
GGCCATGATT ACCAGTGGAT TGGCCTCAAT GACAAGATGT TTGAACATGA CTTCCGCTGG 
ACTGACGGCA GCGCACTGCA ATATGAGAAC TGGAGACCCA ACCAGCCAGA CAGCTTCTTT 
TCTGCTGGAG AAGACTGCGT TGTGATCATT TGGCATGAGA ATGGCCAGTG GAATGACGTC 
CCCTGCAACT ACCACCTCAC CTACACCTGC AAGAAGGGAA CAGTTGCTTG CGGCCAACCC 
CCTGTTGTAG AAAATGCCAA GACCTTTGGA AAGATGAAAC CACGTTATGA AATCAACTCC 
TTGATTAGAT ACCACTGCAA AGATGGTTTC ATTCAGCGTC ACCTTCCAAC TATCCGGTGC 
CTAGGAAATG GGAGATGGGC AATGCCTAAA ATAACCTGCA TGAACCCATC TGCATACCAA 
AGGACTTATT CTAAGAAATA CTTAAAAAAT TCCTCATCAG TCAAGGACAA TTCTATAAAT 
ACGTCAAAAC ATGAGCATCG CTGGAGCCGG AGGTGGCAGG AAACGAGGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Versican localizes to the nucleus in proliferating mesenchymal cells.
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology24(6)368-74(2015 Nov-Dec)
Carthy JM,Abraham T,Meredith AJ,Boroomand S,McManus BM

Expression of V3 Versican by Rat Arterial Smooth Muscle Cells Promotes Differentiated and Anti-inflammatory Phenotypes.
The Journal of biological chemistry290(35)21629-41(2015 Aug)
Kang I,Barth JL,Sproul EP,Yoon DW,Workman GA,Braun KR,Argraves WS,Wight TN

Neuronally produced versican V2 renders C-fiber nociceptors IB4 -positive.
Journal of neurochemistry134(1)147-55(2015 Jul)
Bogen O,Bender O,Löwe J,Blenau W,Thevis B,Schröder W,Margolis RU,Levine JD,Hucho F

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Vcan cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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