Gng3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gng3
Entrez Gene ID	114117
Full Name	G protein subunit gamma 3
General protein information	Preferred Names G protein subunit gamma 3 Names guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3 guanine nucleotide binding protein (G protein), gamma 3 guanine nucleotide binding protein, gamma 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43
Summary	gamma 3 subunit of heterotrimeric G-proteins, which exchange GDP for GTP and activate downstream effectors [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053658.1	NP_446110.1	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gng3	NP_034446.1
Xenopus tropicalis (tropical clawed frog)	gng3	NP_001165080.1
Homo sapiens (human)	GNG3	NP_036334.1
Canis lupus familiaris (dog)	GNG3	XP_540904.2
Bos taurus (cattle)	GNG3	NP_776498.1
Rattus norvegicus (Norway rat)	Gng3	NP_446110.1
Pan troglodytes (chimpanzee)	GNG3	XP_001156854.1
Danio rerio (zebrafish)	gng3	NP_571916.1

Gene Ontology
Bibliography

Related articles in PubMed

Myocyte-specific M-CAT and MEF-1 elements regulate G-protein gamma 3 gene (gamma3) expression in cardiac myocytes.
McWhinney C, Robishaw JD
DNA and cell biology27(7)367-76(2008 Jul)

Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.
Munton RP, Tweedie-Cullen R, Livingstone-Zatchej M, Weinandy F, Waidelich M, Longo D, Gehrig P, Potthast F, Rutishauser D, Gerrits B, Panse C, Schlapbach R, Mansuy IM
Molecular & cellular proteomics : MCP6(2)283-93(2007 Feb)

Developmental expression of heterotrimeric G-proteins in the murine cerebellar cortex.
Schüller U, Lamp EC, Schilling K
Histochemistry and cell biology116(2)149-59(2001 Aug)

Distribution of heterotrimeric G-protein beta and gamma subunits in the rat brain.
Betty M, Harnish SW, Rhodes KJ, Cockett MI
Neuroscience85(2)475-86(1998 Jul)

G protein heterotrimer Galpha13beta1gamma3 couples the angiotensin AT1A receptor to increases in cytoplasmic Ca2+ in rat portal vein myocytes.
Macrez-Leprêtre N, Kalkbrenner F, Morel JL, Schultz G, Mironneau J
The Journal of biological chemistry272(15)10095-102(1997 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gng3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gng3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10228	NM_053658.1 Latest version!	Rattus norvegicus G protein subunit gamma 3 (Gng3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gng3 ( NM_053658.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446110.1 Rattus norvegicus G protein subunit gamma 3 (Gng3), mRNA.

Price & Availability↑

CloneID	ORa10228
Clone ID Related Accession (Same CDS sequence)	NM_053658.1
Accession Version	NM_053658.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527436800000
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC099294.8. On or before Dec 16, 2009 this sequence version replaced XM_001074064.1, XM_001073457.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: FQ212152.1, CF976043.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAAAGGGG AGACCCCCGT TAACAGCACT ATGAGTATTG GCCAAGCACG CAAGATGGTG 
GAACAGCTTA AGATTGAGGC CAGCTTGTGC CGGATAAAGG TGTCCAAGGC AGCAGCAGAC 
CTGATGACTT ACTGTGATGC CCACGCTTGT GAGGATCCCC TCATCACTCC TGTGCCCACT 
TCGGAGAACC CCTTCCGGGA GAAGAAGTTC TTCTGTGCGC TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446110.1
CDS	258..485
Translation	MKGETPVNSTMSIGQARKMVEQLKIEASLCRIKVSKAAADLMTYCDAHACEDPLITPVPTSENPFREKKFFCALL

Target ORF information:

RefSeq Version	NM_053658.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit gamma 3 (Gng3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053658.1

ORF Insert Sequence:

ATGAAAGGGG AGACCCCCGT TAACAGCACT ATGAGTATTG GCCAAGCACG CAAGATGGTG 
GAACAGCTTA AGATTGAGGC CAGCTTGTGC CGGATAAAGG TGTCCAAGGC AGCAGCAGAC 
CTGATGACTT ACTGTGATGC CCACGCTTGT GAGGATCCCC TCATCACTCC TGTGCCCACT 
TCGGAGAACC CCTTCCGGGA GAAGAAGTTC TTCTGTGCGC TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Myocyte-specific M-CAT and MEF-1 elements regulate G-protein gamma 3 gene (gamma3) expression in cardiac myocytes.
DNA and cell biology27(7)367-76(2008 Jul)
McWhinney C,Robishaw JD

Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.
Molecular & cellular proteomics : MCP6(2)283-93(2007 Feb)
Munton RP,Tweedie-Cullen R,Livingstone-Zatchej M,Weinandy F,Waidelich M,Longo D,Gehrig P,Potthast F,Rutishauser D,Gerrits B,Panse C,Schlapbach R,Mansuy IM

Developmental expression of heterotrimeric G-proteins in the murine cerebellar cortex.
Histochemistry and cell biology116(2)149-59(2001 Aug)
Schüller U,Lamp EC,Schilling K

Distribution of heterotrimeric G-protein beta and gamma subunits in the rat brain.
Neuroscience85(2)475-86(1998 Jul)
Betty M,Harnish SW,Rhodes KJ,Cockett MI

G protein heterotrimer Galpha13beta1gamma3 couples the angiotensin AT1A receptor to increases in cytoplasmic Ca2+ in rat portal vein myocytes.
The Journal of biological chemistry272(15)10095-102(1997 Apr)
Macrez-Leprêtre N,Kalkbrenner F,Morel JL,Schultz G,Mironneau J