Epm2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Epm2a
Entrez Gene ID	114005
Full Name	EPM2A, laforin glucan phosphatase
General protein information	Preferred Names EPM2A, laforin glucan phosphatase Names laforin LAFPTPase epilepsy, progressive myoclonic epilepsy, type 2 gene alpha epilepsy, progressive myoclonus type 2A glucan phosphatase lafora PTPase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1p13
Summary	encodes a dual-specificity phosphatase (DSP) named laforin [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276762.1	NP_001263691.1	laforin

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	EPM2A	NP_001026240.1
Mus musculus (house mouse)	Epm2a	NP_034276.2
Danio rerio (zebrafish)	epm2a	XP_003201207.2
Bos taurus (cattle)	EPM2A	NP_001093179.1
Macaca mulatta (Rhesus monkey)	EPM2A	XP_001087462.1
Canis lupus familiaris (dog)	EPM2A	XP_005615541.1
Rattus norvegicus (Norway rat)	Epm2a	NP_001263691.1
Homo sapiens (human)	EPM2A	NP_005661.1
Pan troglodytes (chimpanzee)	EPM2A	XP_001161292.2
Xenopus tropicalis (tropical clawed frog)	epm2a	NP_001123695.1

Gene Ontology
Bibliography

Related articles in PubMed

Suppression of leptin signaling reduces polyglucosan inclusions and seizure susceptibility in a mouse model for Lafora disease.
Rai A, Mishra R, Ganesh S
Human molecular genetics26(24)4778-4785(2017 12)

Abnormal glycogen chain length pattern, not hyperphosphorylation, is critical in Lafora disease.
Nitschke F, Sullivan MA, Wang P, Zhao X, Chown EE, Perri AM, Israelian L, Juana-López L, Bovolenta P, Rodríguez de Córdoba S, Steup M, Minassian BA
EMBO molecular medicine9(7)906-917(2017 07)

Loss of laforin or malin results in increased Drp1 level and concomitant mitochondrial fragmentation in Lafora disease mouse models.
Upadhyay M, Agarwal S, Bhadauriya P, Ganesh S
Neurobiology of disease10039-51(2017 Apr)

Homeostasis of the astrocytic glutamate transporter GLT-1 is altered in mouse models of Lafora disease.
Muñoz-Ballester C, Berthier A, Viana R, Sanz P
Biochimica et biophysica acta1862(6)1074-83(2016 06)

FoxO3a-mediated autophagy is down-regulated in the laforin deficient mice, an animal model for Lafora progressive myoclonus epilepsy.
Jain N, Mishra R, Ganesh S
Biochemical and biophysical research communications474(2)321-327(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Epm2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Epm2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13092	NM_001276762.1 Latest version!	Rattus norvegicus EPM2A glucan phosphatase, laforin (Epm2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Epm2a ( NM_001276762.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001263691.1 Rattus norvegicus EPM2A glucan phosphatase, laforin (Epm2a), mRNA.

Price & Availability↑

CloneID	ORa13092
Clone ID Related Accession (Same CDS sequence)	NM_001276762.1
Accession Version	NM_001276762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571587200000
Organism	Rattus norvegicus(Norway rat)
Product	laforin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07000229.1, AABR07000232.1 and AABR07000235.1. On or before Mar 1, 2013 this sequence version replaced XM_002725446.2, XM_003748648.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF347030.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCTCTTCC GCTTCGGCGT GGTGGTGCCG CCCGCGGTGG CCGGCACTCG GCTGGAGCTG 
CTGCTCGCAG GATCGCGGCC CGAGTTGGGG CGCTGGGAGC CGCGCGGCGC CGTGCGTCTC 
AGGCCGGCGG GTACCGCGGC GGGCGCGGCC GCGCTGGCAT TGCAGGAGCC TGGCCTGTGG 
CTCGCTGAGG TGGAGCTGGC TCCGGAGGAG GAGGCGGCCG ATGGGGCGGA GCCGGGCCGT 
ATTGACACGT TCTGGTACAA GTTCCTGCAG CGCGAGCCGG GAGGCGAGCT GCACTGGGAA 
GGAAATGGAC CTCACCATGA CCGTTGCTGC ACATATAATG AAAACAACTT GGTGGATGGT 
GTTTATTGTC TCCCAGTAGG ACACTGGATT GAGGCCACTG GGCACACCAA CGAAATGAAG 
CACACCACAG ACTTCTATTT TAATATTGCA GGCCACCAAG CCATGCATTA TTCAAGAATT 
CTACCAAATA TCTGGTTGGG TAGCTGCCCT CGACAACTGG AGCATGTGAC CATCAAACTG 
AAGCATGAAC TGGGAATTAC GGCTGTCATG AATTTCCAGA CTGAATGGGA TATCATCCAG 
AATTCTTCAG GCTGCAACCG CTACCCGGAA CCCATGACTC CAGACACCAT GATGAAGCTG 
TATAAGGAAG AAGGCTTGGC CTATATCTGG ATGCCTACTC CAGACATGAG CACTGAGGGC 
CGCGTGCAGA TGCTGCCACA GGCTGTGTGT CTCCTGCACG CGCTTCTGGA GAACGGACAC 
ACGGTGTATG TCCACTGCAA CGCTGGCGTG GGCCGCTCCA CTGCTGCGGT ATGCGGCTGG 
CTCCACTACG TCATCGGCTG GAGTCTGCGC AAGGTGCAGT ACTTCATCAT GGCCAAGAGG 
CCTGCGGTCT ATATCGACGA AGAAGCTTTG GCCCAAGCAC AACAAGACTT TTTTCAGAAG 
TTCGGGAAGG TTCACTCTTC CATATGCACT TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263691.1
CDS	1..996
Translation	MLFRFGVVVPPAVAGTRLELLLAGSRPELGRWEPRGAVRLRPAGTAAGAAALALQEPGLWLAEVELAPEEEAADGAEPGRIDTFWYKFLQREPGGELHWEGNGPHHDRCCTYNENNLVDGVYCLPVGHWIEATGHTNEMKHTTDFYFNIAGHQAMHYSRILPNIWLGSCPRQLEHVTIKLKHELGITAVMNFQTEWDIIQNSSGCNRYPEPMTPDTMMKLYKEEGLAYIWMPTPDMSTEGRVQMLPQAVCLLHALLENGHTVYVHCNAGVGRSTAAVCGWLHYVIGWSLRKVQYFIMAKRPAVYIDEEALAQAQQDFFQKFGKVHSSICTL

Target ORF information:

RefSeq Version	NM_001276762.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus EPM2A glucan phosphatase, laforin (Epm2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276762.1

ORF Insert Sequence:

ATGCTCTTCC GCTTCGGCGT GGTGGTGCCG CCCGCGGTGG CCGGCACTCG GCTGGAGCTG 
CTGCTCGCAG GATCGCGGCC CGAGTTGGGG CGCTGGGAGC CGCGCGGCGC CGTGCGTCTC 
AGGCCGGCGG GTACCGCGGC GGGCGCGGCC GCGCTGGCAT TGCAGGAGCC TGGCCTGTGG 
CTCGCTGAGG TGGAGCTGGC TCCGGAGGAG GAGGCGGCCG ATGGGGCGGA GCCGGGCCGT 
ATTGACACGT TCTGGTACAA GTTCCTGCAG CGCGAGCCGG GAGGCGAGCT GCACTGGGAA 
GGAAATGGAC CTCACCATGA CCGTTGCTGC ACATATAATG AAAACAACTT GGTGGATGGT 
GTTTATTGTC TCCCAGTAGG ACACTGGATT GAGGCCACTG GGCACACCAA CGAAATGAAG 
CACACCACAG ACTTCTATTT TAATATTGCA GGCCACCAAG CCATGCATTA TTCAAGAATT 
CTACCAAATA TCTGGTTGGG TAGCTGCCCT CGACAACTGG AGCATGTGAC CATCAAACTG 
AAGCATGAAC TGGGAATTAC GGCTGTCATG AATTTCCAGA CTGAATGGGA TATCATCCAG 
AATTCTTCAG GCTGCAACCG CTACCCGGAA CCCATGACTC CAGACACCAT GATGAAGCTG 
TATAAGGAAG AAGGCTTGGC CTATATCTGG ATGCCTACTC CAGACATGAG CACTGAGGGC 
CGCGTGCAGA TGCTGCCACA GGCTGTGTGT CTCCTGCACG CGCTTCTGGA GAACGGACAC 
ACGGTGTATG TCCACTGCAA CGCTGGCGTG GGCCGCTCCA CTGCTGCGGT ATGCGGCTGG 
CTCCACTACG TCATCGGCTG GAGTCTGCGC AAGGTGCAGT ACTTCATCAT GGCCAAGAGG 
CCTGCGGTCT ATATCGACGA AGAAGCTTTG GCCCAAGCAC AACAAGACTT TTTTCAGAAG 
TTCGGGAAGG TTCACTCTTC CATATGCACT TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Suppression of leptin signaling reduces polyglucosan inclusions and seizure susceptibility in a mouse model for Lafora disease.
Human molecular genetics26(24)4778-4785(2017 12)
Rai A,Mishra R,Ganesh S

Abnormal glycogen chain length pattern, not hyperphosphorylation, is critical in Lafora disease.
EMBO molecular medicine9(7)906-917(2017 07)
Nitschke F,Sullivan MA,Wang P,Zhao X,Chown EE,Perri AM,Israelian L,Juana-López L,Bovolenta P,Rodríguez de Córdoba S,Steup M,Minassian BA

Loss of laforin or malin results in increased Drp1 level and concomitant mitochondrial fragmentation in Lafora disease mouse models.
Neurobiology of disease10039-51(2017 Apr)
Upadhyay M,Agarwal S,Bhadauriya P,Ganesh S

Homeostasis of the astrocytic glutamate transporter GLT-1 is altered in mouse models of Lafora disease.
Biochimica et biophysica acta1862(6)1074-83(2016 06)
Muñoz-Ballester C,Berthier A,Viana R,Sanz P

FoxO3a-mediated autophagy is down-regulated in the laforin deficient mice, an animal model for Lafora progressive myoclonus epilepsy.
Biochemical and biophysical research communications474(2)321-327(2016 05)
Jain N,Mishra R,Ganesh S