GRAMD4 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRAMD4
Entrez Gene ID	105305889
Full Name	GRAM domain containing 4
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011380733.1	XP_011379035.1	GRAM domain-containing protein 4
XM_023523687.1	XP_023379455.1	GRAM domain-containing protein 4

Pathways from BioSystems
Gene Ontology

The following GRAMD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRAMD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPt61615	XM_011380733.1 Latest version!	Pteropus vampyrus GRAM domain containing 4 (GRAMD4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OPt77244	XM_023523687.1 Latest version!	Pteropus vampyrus GRAM domain containing 4 (GRAMD4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRAMD4 ( XM_011380733.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011379035.1 Pteropus vampyrus GRAM domain containing 4 (GRAMD4), mRNA.

Price & Availability↑

CloneID	OPt61615
Clone ID Related Accession (Same CDS sequence)	XM_011380733.1
Accession Version	XM_011380733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	GRAM domain-containing protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011889182.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 13% of CDS bases ##RefSeq-Attributes-END##

ATGCTCAGGA GGCTGGACAG GATCAGGTTC AGAGGTCACA GGAGAGATGA CTTCCTCGAT 
CTAGCGGAGT CTCCCAATGC CTCGGACACC GAGTGCGGAG ACGAAGGCCC CCTGAGAACG 
CCTCGGACCT CGCCCCGCGA CAGCGAGGAG CTTCGGGACC CCGCCGGCCC GGGTACGCTC 
ATCATGGCCG CGGGCGTCCA GGACCTTAAC CGGACGGAGT TCGATCGATT AAACGAGATC 
AAAGGTCACC TGGAGATCGC CTTGCTGGAG AAACACTTCC TGCATGTGGC CTGGAGAACC 
CCTGCCCCCG ACCACGATGA TTACATTGTC ACCTGTGTCC TGTCGCGTGG CCGCGGGTCC 
CTCCAGGCCT GCCGCGTGGT CGGCACCGTC ACTGGTCCCC CCCGGACCTG CCGCATGGCT 
GCGGGCTCTG TTTTTGGGGG GCCAAGCCCC CCACAGCCTG GCCCCCACCT CGGTGCTTCC 
TCACCCCACC CCCTGCAAGC CCTCCAGGCC CCGCCCTCAC TGATGCCTGC CGTCTCTGCA 
GACCGGCGGG GCCAGGGGCT GTGCCCCCGC GTGCAGAAGT GGTTTTTCGA GAAGTTCGGG 
GAGTACGTGG AGGACTTCCG GTTCCAGCCC GAGGAGAGTG CCGTGGAGAC GGAGGAGCCC 
CTCAGCGCCC GCAGATTAAC CGAGAACATG CGGCGACTGA GTGAGTACCG TGCCCTTGGG 
GGGCTGACCA GTGCGTGGGG GCCCCGATGC ATGAACTGCC CGGTGGCCGT CTGTCCTCTC 
AAAGCCAGTG CCACTCTCCA GTCATGTGGC ACCGCACCTG GGCGGGCCGG GACCCCAGCG 
AGGCGGGCCC TCTCTGCTCC CGGGCCGCGG CCCTCTCCCC GGCAACCCGT ATGTCCCACC 
ACCTCCCGAG GGGCTCCCCC TTCACGTCCT CATGGGCAGC TCCTCTGCTT CCCCAGGCGC 
AAGCCTGCAG GGCAAGGTGG GGTGCGGTCT GCCTCGAGCT GCCGTTGGGC CCCTCACCGT 
GTCCCCACTG CCCCGTCCTG CCCGCAGGTC TACGTGAACG CCGTGTGGCA TGGCTGGGCC 
ATCCCCATGT TCTTATTTCT GGCAATTCTG CGGCTGTCCC TCAATTACCT CATAGCCAGG 
GGCTGGAGGA TACAGTGGAG CATCGTCCCT GAAGTGTCCG AGGTGGTGGA GCCTCCGAAG 
GAAGACCTGA CCGTGTCTGA GAAGTTCCAG CTCGTGCTGG ACGTCGCCCA GAAGGCCCAG 
AACCTCTTTG GCAAGATGGC CGACATCCTG GAAAAGATCA AGAACCTGTT CATGTGGGTG 
CAGCCGGAGA TCACGCAGAA GCTGTACGTC GCGCTGTGGG CCGCCTTCCT GGCTTCCTGC 
TTCTTCCCCT ACCGCCTGGT GGGCCTCGCC CTGGGTAAGG CGCCGCTCGG CCGGGTGAGG 
GCAGAGCGGG CTGAGGGGTG GGCGTCCGGG GCGGTGGCAG TCCTGCGAGG CGTCGCGGGC 
CTGCGTCTGC ACTTGCAGCC CGCTGGGCCG GCGTCGCGTG GCCCGGCCTT TTCTTTCGTC 
TTGGTCCCAC GTGTGTCCGT TGTCTCTTTT CTGACCAGCT GTCAGGGCAT GACAAGGTAC 
GGTGTCCGCG GGGAAGCCCC TCCTCCTGCC GTGACCCAGT GCTGTGTGTT GCCGTGCTGT 
GACTGTGCCG TGACTGTGAC GTGTGTGTTG CTTCTGGTGG CCTGTCGCGG GCCAGCTCTG 
ATCCTGGCAC GTCTGTCTGA ACTGCCAGCG CTGAGCACCT GTGGCACAGC ACCTGGACTG 
TGTGTGGGGG CAGAGGGTCT CCCCACGGCC ACAGGGCGCC GAGGACCGGG CACCCCATGC 
CTGGGTGCCC ACGCCTGGGC GCACACGCTG TTTGCCCGTC CGTTGGCCTC TGGGCGTGGG 
GAAGGGGGTC GTGTGCGTGC CGGCTCCTTG CCCCCCTTGG CATGGGCATG GCTGACGGCG 
GGACGCTCTT GGCAGCCGCA GACGGCCTCG CGGAGCTACG TGTCCAGTGC GCCAGCCAGC 
CTGAGCAAGG ACGAAGACGC CGGCCGCTTC CACAGCACCA AGAAGGGCAA CTTCCACGAG 
ATCTTCAACC TGACGGAAAA TGAGCGACCG CTGGCGGCAT GTGAGAACGG CTGGCGTTGC 
TGCCTCATAA ACAGAGACCG GAAGATGCCC ACGGACTACA TCAGGAACGG GCTGCTGTAC 
GTGACCGAGA ATTACTTGTG CTTCGAGAGC TCCAAGTCCG GCTCCTCCAA GAGGAACAAA 
GTCATCAAGC TGGTGGACAT CACAGACATT CAGAAGTACA AGGTCCTTTC TGTCCTCCCG 
GGGTCTGGCA TGGGGATCGC CGTGTCGACG CCATCGACCC AGAAACCGCT GGTGTTCGGT 
GCCATGGTAC ACAGAGACGA GGCCTTCGAG ACCATCTTCA GCCAGTATGT GAAAATCACA 
GCAGCGGCAG CTTCCGGCAG TGACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011379035.1
CDS	50..2539
Translation	MLRRLDRIRFRGHRRDDFLDLAESPNASDTECGDEGPLRTPRTSPRDSEELRDPAGPGTLIMAAGVQDLNRTEFDRLNEIKGHLEIALLEKHFLHVAWRTPAPDHDDYIVTCVLSRGRGSLQACRVVGTVTGPPRTCRMAAGSVFGGPSPPQPGPHLGASSPHPLQALQAPPSLMPAVSADRRGQGLCPRVQKWFFEKFGEYVEDFRFQPEESAVETEEPLSARRLTENMRRLSEYRALGGLTSAWGPRCMNCPVAVCPLKASATLQSCGTAPGRAGTPARRALSAPGPRPSPRQPVCPTTSRGAPPSRPHGQLLCFPRRKPAGQGGVRSASSCRWAPHRVPTAPSCPQVYVNAVWHGWAIPMFLFLAILRLSLNYLIARGWRIQWSIVPEVSEVVEPPKEDLTVSEKFQLVLDVAQKAQNLFGKMADILEKIKNLFMWVQPEITQKLYVALWAAFLASCFFPYRLVGLALGKAPLGRVRAERAEGWASGAVAVLRGVAGLRLHLQPAGPASRGPAFSFVLVPRVSVVSFLTSCQGMTRYGVRGEAPPPAVTQCCVLPCCDCAVTVTCVLLLVACRGPALILARLSELPALSTCGTAPGLCVGAEGLPTATGRRGPGTPCLGAHAWAHTLFARPLASGRGEGGRVRAGSLPPLAWAWLTAGRSWQPQTASRSYVSSAPASLSKDEDAGRFHSTKKGNFHEIFNLTENERPLAACENGWRCCLINRDRKMPTDYIRNGLLYVTENYLCFESSKSGSSKRNKVIKLVDITDIQKYKVLSVLPGSGMGIAVSTPSTQKPLVFGAMVHRDEAFETIFSQYVKITAAAASGSDS

Target ORF information:

RefSeq Version	XM_011380733.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus GRAM domain containing 4 (GRAMD4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011380733.1

ORF Insert Sequence:

ATGCTCAGGA GGCTGGACAG GATCAGGTTC AGAGGTCACA GGAGAGATGA CTTCCTCGAT 
CTAGCGGAGT CTCCCAATGC CTCGGACACC GAGTGCGGAG ACGAAGGCCC CCTGAGAACG 
CCTCGGACCT CGCCCCGCGA CAGCGAGGAG CTTCGGGACC CCGCCGGCCC GGGTACGCTC 
ATCATGGCCG CGGGCGTCCA GGACCTTAAC CGGACGGAGT TCGATCGATT AAACGAGATC 
AAAGGTCACC TGGAGATCGC CTTGCTGGAG AAACACTTCC TGCATGTGGC CTGGAGAACC 
CCTGCCCCCG ACCACGATGA TTACATTGTC ACCTGTGTCC TGTCGCGTGG CCGCGGGTCC 
CTCCAGGCCT GCCGCGTGGT CGGCACCGTC ACTGGTCCCC CCCGGACCTG CCGCATGGCT 
GCGGGCTCTG TTTTTGGGGG GCCAAGCCCC CCACAGCCTG GCCCCCACCT CGGTGCTTCC 
TCACCCCACC CCCTGCAAGC CCTCCAGGCC CCGCCCTCAC TGATGCCTGC CGTCTCTGCA 
GACCGGCGGG GCCAGGGGCT GTGCCCCCGC GTGCAGAAGT GGTTTTTCGA GAAGTTCGGG 
GAGTACGTGG AGGACTTCCG GTTCCAGCCC GAGGAGAGTG CCGTGGAGAC GGAGGAGCCC 
CTCAGCGCCC GCAGATTAAC CGAGAACATG CGGCGACTGA GTGAGTACCG TGCCCTTGGG 
GGGCTGACCA GTGCGTGGGG GCCCCGATGC ATGAACTGCC CGGTGGCCGT CTGTCCTCTC 
AAAGCCAGTG CCACTCTCCA GTCATGTGGC ACCGCACCTG GGCGGGCCGG GACCCCAGCG 
AGGCGGGCCC TCTCTGCTCC CGGGCCGCGG CCCTCTCCCC GGCAACCCGT ATGTCCCACC 
ACCTCCCGAG GGGCTCCCCC TTCACGTCCT CATGGGCAGC TCCTCTGCTT CCCCAGGCGC 
AAGCCTGCAG GGCAAGGTGG GGTGCGGTCT GCCTCGAGCT GCCGTTGGGC CCCTCACCGT 
GTCCCCACTG CCCCGTCCTG CCCGCAGGTC TACGTGAACG CCGTGTGGCA TGGCTGGGCC 
ATCCCCATGT TCTTATTTCT GGCAATTCTG CGGCTGTCCC TCAATTACCT CATAGCCAGG 
GGCTGGAGGA TACAGTGGAG CATCGTCCCT GAAGTGTCCG AGGTGGTGGA GCCTCCGAAG 
GAAGACCTGA CCGTGTCTGA GAAGTTCCAG CTCGTGCTGG ACGTCGCCCA GAAGGCCCAG 
AACCTCTTTG GCAAGATGGC CGACATCCTG GAAAAGATCA AGAACCTGTT CATGTGGGTG 
CAGCCGGAGA TCACGCAGAA GCTGTACGTC GCGCTGTGGG CCGCCTTCCT GGCTTCCTGC 
TTCTTCCCCT ACCGCCTGGT GGGCCTCGCC CTGGGTAAGG CGCCGCTCGG CCGGGTGAGG 
GCAGAGCGGG CTGAGGGGTG GGCGTCCGGG GCGGTGGCAG TCCTGCGAGG CGTCGCGGGC 
CTGCGTCTGC ACTTGCAGCC CGCTGGGCCG GCGTCGCGTG GCCCGGCCTT TTCTTTCGTC 
TTGGTCCCAC GTGTGTCCGT TGTCTCTTTT CTGACCAGCT GTCAGGGCAT GACAAGGTAC 
GGTGTCCGCG GGGAAGCCCC TCCTCCTGCC GTGACCCAGT GCTGTGTGTT GCCGTGCTGT 
GACTGTGCCG TGACTGTGAC GTGTGTGTTG CTTCTGGTGG CCTGTCGCGG GCCAGCTCTG 
ATCCTGGCAC GTCTGTCTGA ACTGCCAGCG CTGAGCACCT GTGGCACAGC ACCTGGACTG 
TGTGTGGGGG CAGAGGGTCT CCCCACGGCC ACAGGGCGCC GAGGACCGGG CACCCCATGC 
CTGGGTGCCC ACGCCTGGGC GCACACGCTG TTTGCCCGTC CGTTGGCCTC TGGGCGTGGG 
GAAGGGGGTC GTGTGCGTGC CGGCTCCTTG CCCCCCTTGG CATGGGCATG GCTGACGGCG 
GGACGCTCTT GGCAGCCGCA GACGGCCTCG CGGAGCTACG TGTCCAGTGC GCCAGCCAGC 
CTGAGCAAGG ACGAAGACGC CGGCCGCTTC CACAGCACCA AGAAGGGCAA CTTCCACGAG 
ATCTTCAACC TGACGGAAAA TGAGCGACCG CTGGCGGCAT GTGAGAACGG CTGGCGTTGC 
TGCCTCATAA ACAGAGACCG GAAGATGCCC ACGGACTACA TCAGGAACGG GCTGCTGTAC 
GTGACCGAGA ATTACTTGTG CTTCGAGAGC TCCAAGTCCG GCTCCTCCAA GAGGAACAAA 
GTCATCAAGC TGGTGGACAT CACAGACATT CAGAAGTACA AGGTCCTTTC TGTCCTCCCG 
GGGTCTGGCA TGGGGATCGC CGTGTCGACG CCATCGACCC AGAAACCGCT GGTGTTCGGT 
GCCATGGTAC ACAGAGACGA GGCCTTCGAG ACCATCTTCA GCCAGTATGT GAAAATCACA 
GCAGCGGCAG CTTCCGGCAG TGACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD4 ( XM_023523687.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023379455.1 Pteropus vampyrus GRAM domain containing 4 (GRAMD4), mRNA.

Price & Availability↑

CloneID	OPt77244
Clone ID Related Accession (Same CDS sequence)	XM_023523687.1
Accession Version	XM_023523687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	GRAM domain-containing protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011889182.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 11% of CDS bases ##RefSeq-Attributes-END##

ATGCTCAGGA GGCTGGACAG GATCAGGTTC AGAGGTCACA GGAGAGATGA CTTCCTCGAT 
CTAGCGGAGT CTCCCAATGC CTCGGACACC GAGTGCGGAG ACGAAGGCCC CCTGAGAACG 
CCTCGGACCT CGCCCCGCGA CAGCGAGGAG CTTCGGGACC CCGCCGGCCC GGGTACGCTC 
ATCATGGCCG CGGGCGTCCA GGACCTTAAC CGGACGGAGT TCGATCGATT AAACGAGATC 
AAAGGTCACC TGGAGATCGC CTTGCTGGAG AAACACTTCC TGCATGTGGC CTGGAGAACC 
CCTGCCCCCG ACCACGATGA TTACATTGTC ACCTGTGTCC TGTCGCGTGG CCGCGGGTCC 
CTCCAGGCCT GCCGCGTGGT CGGCACCGTC ACTGGTCCCC CCCGGACCTG CCGCATGGCT 
GCGGGGGGAG GGGGGCTGAC ACAGGCCCCC GTGGGCTGGG AGCTGCCTGG ACAGTGCTTG 
TGCTGGGGAC AGCCTCAGGC CAGTGGGAGT CAGTGTGAGG GACACCAAGG CCCCCCATGT 
GGTCTGCGTG GGCTGGAGTT GCTCCTCGGC GGGTCAGGCT CTGTTTTTGG GGGGCCAAGC 
CCCCCACAGC CTGGCCCCCA CCTCGGTGCT TCCTCACCCC ACCCCCTGCA AGCCCTCCAG 
GCCCCGCCCT CACTGATGCC TGCCGTCTCT GCAGACCGGC GGGGCCAGGG GCTGTGCCCC 
CGCGTGCAGA AGTGGTTTTT CGAGAAGTTC GGGGAGTACG TGGAGGACTT CCGGTTCCAG 
CCCGAGGAGA GTGCCGTGGA GACGGAGGAG CCCCTCAGCG CCCGCAGATT AACCGAGAAC 
ATGCGGCGAC TGAGTGAGTA CCGTGCCCTT GGGGGGCTGA CCAGTGCGTG GGGGCCCCGA 
GCGGGTGTAA GGAGGATGCA GGCCACCCAC CTGCCTGTGG ATTCCCGAGG CCAGCAACAG 
GTGGCCACAG CTCCCCAAGC TCTAGCATGT GGCACCGCAC CTGGGCGGGC CGGGACCCCA 
GCGAGGCGGG CCCTCTCTGC TCCCGGGCCG CGGCCCTCTC CCCGGCAACC CGTATGTCCC 
ACCACCTCCC GAGGGGCTCC CCCTTCACGT CCTCATGGGC AGCTCCTCTG CTTCCCCAGG 
CGCAAGCCTG CAGGGCAAGG TGGGGTGCGG TCTGCCTCGA GCTGCCGTTG GGCCCCTCAC 
CGTGTCCCCA CTGCCCCGTC CTGCCCGCAG GTCTACGTGA ACGCCGTGTG GCATGGCTGG 
GCCATCCCCA TGTTCTTATT TCTGGCAATT CTGCGGCTGT CCCTCAATTA CCTCATAGCC 
AGGGGCTGGA GGATACAGTG GAGCATCGTC CCTGAAGTGT CCGAGGTGGT GGAGCCTCCG 
AAGGAAGACC TGACCGTGTC TGAGAAGTTC CAGCTCGTGC TGGACGTCGC CCAGAAGGCC 
CAGAACCTCT TTGGCAAGAT GGCCGACATC CTGGAAAAGA TCAAGAACCT GTTCATGTGG 
GTGCAGCCGG AGATCACGCA GAAGCTGTAC GTCGCGCTGT GGGCCGCCTT CCTGGCTTCC 
TGCTTCTTCC CCTACCGCCT GGTGGGCCTC GCCCTGGGTA AGGCGCCGCT CGGCCGGTGC 
GGCCGGGCTG GGGCGGCCGG GCCGCTGCTT CCCACCGGGT GTCTGCGGCA CCGCCGCCTC 
CAGGGAGGAG GCCGGCTGTT CACTCTCACC GCGTTCCTTC CTCTGCGCCT CGGCCAGTGG 
TTTTTATCAA AACGCTTAAA TTGGGGAGAA ATGAAGGTGA GGGCAGAGCG GGCTGAGGGG 
TGGGCGTCCG GGGCGGTGGC AGTCCTGCGA GGCGTCGCGG GCCTGCGTCT GCACTTGCAG 
CCCGCTGGGC CGGCGTCGCG TGGCCCGGCC TTTTCTTTCG TCTTGGTCCC ACGTGTGTCC 
GTTGTCTCTT TTCTGACCAG CTGTCAGGGC ATGACAAGGT ACGGTGTCCG CGGGGAAGCC 
CCTCCTCCTG CCGTGACCCA GTGCTGTGTG TTGCCGTGCT GTGACTGTGC CGTGACTGTG 
ACGTGTGTGT TGCTTCTGGT GGCCTGTCGC GGGCCAGCTC TGATCCTGGC ACGTCTGTCT 
GAACTGCCAG CGCTGAGCAC CTGTGGCACA GCACCTGGAC TGTGTGTGGG GGCAGAGGGT 
CTCCCCACGG CCACAGGGCG CCGAGGACCG GGCACCCCAT GCCTGGGTGC CCACGCCTGG 
GCGCACACGC TGTTTGCCCG TCCGTTGGCC TCTGGGCGTG GGGAAGGGGG TCGTGTGCGT 
GCCGGCTCCT TGCCCCCCTT GGCATGGGCA TGGCTGACGG CGGGACGCTC TTGGCAGCCG 
CAGACGGCCT CGCGGAGCTA CGTGTCCAGT GCGCCAGCCA GCCTGAGCAA GGACGAAGAC 
GCCGGCCGCT TCCACAGCAC CAAGAAGGGC AACTTCCACG AGATCTTCAA CCTGACGGAA 
AATGAGCGAC CGCTGGCGGC ATGTGAGAAC GGCTGGCGTT GCTGCCTCAT AAACAGAGAC 
CGGAAGATGC CCACGGACTA CATCAGGAAC GGGCTGCTGT ACGTGACCGA GAATTACTTG 
TGCTTCGAGA GCTCCAAGTC CGGCTCCTCC AAGAGGAACA AAGTCATCAA GCTGGTGGAC 
ATCACAGACA TTCAGAAGTA CAAGGTCCTT TCTGTCCTCC CGGGGTCTGG CATGGGGATC 
GCCGTGTCGA CGCCATCGAC CCAGAAACCG CTGGTGTTCG GTGCCATGGT ACACAGAGAC 
GAGGCCTTCG AGACCATCTT CAGCCAGTAT GTGAAAATCA CAGCAGCGGC AGCTTCCGGC 
AGTGACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023379455.1
CDS	326..3157
Translation	MLRRLDRIRFRGHRRDDFLDLAESPNASDTECGDEGPLRTPRTSPRDSEELRDPAGPGTLIMAAGVQDLNRTEFDRLNEIKGHLEIALLEKHFLHVAWRTPAPDHDDYIVTCVLSRGRGSLQACRVVGTVTGPPRTCRMAAGGGGLTQAPVGWELPGQCLCWGQPQASGSQCEGHQGPPCGLRGLELLLGGSGSVFGGPSPPQPGPHLGASSPHPLQALQAPPSLMPAVSADRRGQGLCPRVQKWFFEKFGEYVEDFRFQPEESAVETEEPLSARRLTENMRRLSEYRALGGLTSAWGPRAGVRRMQATHLPVDSRGQQQVATAPQALACGTAPGRAGTPARRALSAPGPRPSPRQPVCPTTSRGAPPSRPHGQLLCFPRRKPAGQGGVRSASSCRWAPHRVPTAPSCPQVYVNAVWHGWAIPMFLFLAILRLSLNYLIARGWRIQWSIVPEVSEVVEPPKEDLTVSEKFQLVLDVAQKAQNLFGKMADILEKIKNLFMWVQPEITQKLYVALWAAFLASCFFPYRLVGLALGKAPLGRCGRAGAAGPLLPTGCLRHRRLQGGGRLFTLTAFLPLRLGQWFLSKRLNWGEMKVRAERAEGWASGAVAVLRGVAGLRLHLQPAGPASRGPAFSFVLVPRVSVVSFLTSCQGMTRYGVRGEAPPPAVTQCCVLPCCDCAVTVTCVLLLVACRGPALILARLSELPALSTCGTAPGLCVGAEGLPTATGRRGPGTPCLGAHAWAHTLFARPLASGRGEGGRVRAGSLPPLAWAWLTAGRSWQPQTASRSYVSSAPASLSKDEDAGRFHSTKKGNFHEIFNLTENERPLAACENGWRCCLINRDRKMPTDYIRNGLLYVTENYLCFESSKSGSSKRNKVIKLVDITDIQKYKVLSVLPGSGMGIAVSTPSTQKPLVFGAMVHRDEAFETIFSQYVKITAAAASGSDS

Target ORF information:

RefSeq Version	XM_023523687.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus GRAM domain containing 4 (GRAMD4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023523687.1

ORF Insert Sequence:

ATGCTCAGGA GGCTGGACAG GATCAGGTTC AGAGGTCACA GGAGAGATGA CTTCCTCGAT 
CTAGCGGAGT CTCCCAATGC CTCGGACACC GAGTGCGGAG ACGAAGGCCC CCTGAGAACG 
CCTCGGACCT CGCCCCGCGA CAGCGAGGAG CTTCGGGACC CCGCCGGCCC GGGTACGCTC 
ATCATGGCCG CGGGCGTCCA GGACCTTAAC CGGACGGAGT TCGATCGATT AAACGAGATC 
AAAGGTCACC TGGAGATCGC CTTGCTGGAG AAACACTTCC TGCATGTGGC CTGGAGAACC 
CCTGCCCCCG ACCACGATGA TTACATTGTC ACCTGTGTCC TGTCGCGTGG CCGCGGGTCC 
CTCCAGGCCT GCCGCGTGGT CGGCACCGTC ACTGGTCCCC CCCGGACCTG CCGCATGGCT 
GCGGGGGGAG GGGGGCTGAC ACAGGCCCCC GTGGGCTGGG AGCTGCCTGG ACAGTGCTTG 
TGCTGGGGAC AGCCTCAGGC CAGTGGGAGT CAGTGTGAGG GACACCAAGG CCCCCCATGT 
GGTCTGCGTG GGCTGGAGTT GCTCCTCGGC GGGTCAGGCT CTGTTTTTGG GGGGCCAAGC 
CCCCCACAGC CTGGCCCCCA CCTCGGTGCT TCCTCACCCC ACCCCCTGCA AGCCCTCCAG 
GCCCCGCCCT CACTGATGCC TGCCGTCTCT GCAGACCGGC GGGGCCAGGG GCTGTGCCCC 
CGCGTGCAGA AGTGGTTTTT CGAGAAGTTC GGGGAGTACG TGGAGGACTT CCGGTTCCAG 
CCCGAGGAGA GTGCCGTGGA GACGGAGGAG CCCCTCAGCG CCCGCAGATT AACCGAGAAC 
ATGCGGCGAC TGAGTGAGTA CCGTGCCCTT GGGGGGCTGA CCAGTGCGTG GGGGCCCCGA 
GCGGGTGTAA GGAGGATGCA GGCCACCCAC CTGCCTGTGG ATTCCCGAGG CCAGCAACAG 
GTGGCCACAG CTCCCCAAGC TCTAGCATGT GGCACCGCAC CTGGGCGGGC CGGGACCCCA 
GCGAGGCGGG CCCTCTCTGC TCCCGGGCCG CGGCCCTCTC CCCGGCAACC CGTATGTCCC 
ACCACCTCCC GAGGGGCTCC CCCTTCACGT CCTCATGGGC AGCTCCTCTG CTTCCCCAGG 
CGCAAGCCTG CAGGGCAAGG TGGGGTGCGG TCTGCCTCGA GCTGCCGTTG GGCCCCTCAC 
CGTGTCCCCA CTGCCCCGTC CTGCCCGCAG GTCTACGTGA ACGCCGTGTG GCATGGCTGG 
GCCATCCCCA TGTTCTTATT TCTGGCAATT CTGCGGCTGT CCCTCAATTA CCTCATAGCC 
AGGGGCTGGA GGATACAGTG GAGCATCGTC CCTGAAGTGT CCGAGGTGGT GGAGCCTCCG 
AAGGAAGACC TGACCGTGTC TGAGAAGTTC CAGCTCGTGC TGGACGTCGC CCAGAAGGCC 
CAGAACCTCT TTGGCAAGAT GGCCGACATC CTGGAAAAGA TCAAGAACCT GTTCATGTGG 
GTGCAGCCGG AGATCACGCA GAAGCTGTAC GTCGCGCTGT GGGCCGCCTT CCTGGCTTCC 
TGCTTCTTCC CCTACCGCCT GGTGGGCCTC GCCCTGGGTA AGGCGCCGCT CGGCCGGTGC 
GGCCGGGCTG GGGCGGCCGG GCCGCTGCTT CCCACCGGGT GTCTGCGGCA CCGCCGCCTC 
CAGGGAGGAG GCCGGCTGTT CACTCTCACC GCGTTCCTTC CTCTGCGCCT CGGCCAGTGG 
TTTTTATCAA AACGCTTAAA TTGGGGAGAA ATGAAGGTGA GGGCAGAGCG GGCTGAGGGG 
TGGGCGTCCG GGGCGGTGGC AGTCCTGCGA GGCGTCGCGG GCCTGCGTCT GCACTTGCAG 
CCCGCTGGGC CGGCGTCGCG TGGCCCGGCC TTTTCTTTCG TCTTGGTCCC ACGTGTGTCC 
GTTGTCTCTT TTCTGACCAG CTGTCAGGGC ATGACAAGGT ACGGTGTCCG CGGGGAAGCC 
CCTCCTCCTG CCGTGACCCA GTGCTGTGTG TTGCCGTGCT GTGACTGTGC CGTGACTGTG 
ACGTGTGTGT TGCTTCTGGT GGCCTGTCGC GGGCCAGCTC TGATCCTGGC ACGTCTGTCT 
GAACTGCCAG CGCTGAGCAC CTGTGGCACA GCACCTGGAC TGTGTGTGGG GGCAGAGGGT 
CTCCCCACGG CCACAGGGCG CCGAGGACCG GGCACCCCAT GCCTGGGTGC CCACGCCTGG 
GCGCACACGC TGTTTGCCCG TCCGTTGGCC TCTGGGCGTG GGGAAGGGGG TCGTGTGCGT 
GCCGGCTCCT TGCCCCCCTT GGCATGGGCA TGGCTGACGG CGGGACGCTC TTGGCAGCCG 
CAGACGGCCT CGCGGAGCTA CGTGTCCAGT GCGCCAGCCA GCCTGAGCAA GGACGAAGAC 
GCCGGCCGCT TCCACAGCAC CAAGAAGGGC AACTTCCACG AGATCTTCAA CCTGACGGAA 
AATGAGCGAC CGCTGGCGGC ATGTGAGAAC GGCTGGCGTT GCTGCCTCAT AAACAGAGAC 
CGGAAGATGC CCACGGACTA CATCAGGAAC GGGCTGCTGT ACGTGACCGA GAATTACTTG 
TGCTTCGAGA GCTCCAAGTC CGGCTCCTCC AAGAGGAACA AAGTCATCAA GCTGGTGGAC 
ATCACAGACA TTCAGAAGTA CAAGGTCCTT TCTGTCCTCC CGGGGTCTGG CATGGGGATC 
GCCGTGTCGA CGCCATCGAC CCAGAAACCG CTGGTGTTCG GTGCCATGGT ACACAGAGAC 
GAGGCCTTCG AGACCATCTT CAGCCAGTAT GTGAAAATCA CAGCAGCGGC AGCTTCCGGC 
AGTGACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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GRAMD4 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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