MAP3K1 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K1
Entrez Gene ID	105299360
Full Name	mitogen-activated protein kinase kinase kinase 1
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011371048.1	XP_011369350.1	mitogen-activated protein kinase kinase kinase 1
XM_023537199.1	XP_023392967.1	mitogen-activated protein kinase kinase kinase 1 isoform X3
XM_023537198.1	XP_023392966.1	mitogen-activated protein kinase kinase kinase 1 isoform X2
XM_011371048.2	XP_011369350.1	mitogen-activated protein kinase kinase kinase 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following MAP3K1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt73638	XM_023537198.1 Latest version!	Pteropus vampyrus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPt73639	XM_023537199.1 Latest version!	Pteropus vampyrus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPt54651	XM_011371048.2 Latest version!	Pteropus vampyrus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OPt54651	XM_011371048.1	Pteropus vampyrus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K1 ( XM_023537198.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023392966.1 Pteropus vampyrus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPt73638
Clone ID Related Accession (Same CDS sequence)	XM_023537198.1
Accession Version	XM_023537198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888962.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCAGC CATTCAGTTT CAAAGCGGTG GTGACTGCTC CCCGCAGCCT CTCCGGCGTG 
GCTGGAGACC GTGGCGCGGG TGCGGTGCTT CCTGCTCCGT GCAGCTGCGC GGGCTGCCCG 
CGGACTGCCG GATTCCAGGG GCCACAGTGC AAGTGGGCTT CGGCTCAGAT AGTTCTGTGC 
TGCCTGTGCA GTACGGTATG TGTAAGTCGT GAGATGGAGA ATAAGGAAAC TCTCAAGGGT 
TTGCACAAGA TGGATGATCG CCCAGAAGAA CGAATGATCA GGGAGAAACT GAAGGCAACC 
TGTATGCCAG CCTGGAAGCA CGAATGGCTG GAAAGGAGAA ATAAGAGAGG CCCTGTGGTG 
GTAAAACCAA TCCCTATTAA AGGAGATGGA TCTGAAATTA ATAACTTGGC AACTGAGTCT 
CAAGGAGAGG GCCAGGCGAG TACCACTTCA CCAGCTCCCA AAGGCCGACG CAGTCCTTCC 
CCTGGCAGCT CCCCATCAGG TCGCACAGTG AAACCAGACT CTCCAGGAGT AAGGAGAAAA 
AGAGTTTCCC CCGTGCCATT TCAGAGTGGG AGAATCACAC CACCTCGAAG AGCCCCTTCA 
CCAGATGGCT TCTCACCATA CAGCCCTGAG GAAACAAACC GCCGTGTAAA CAAAGTGATG 
CGGGCCAGGC TGTACTTACT GCAGCAAATA GGACCCAACT CTTTCCTGAT TGGAGGAGAT 
AGTCCAGACA ATAAATACCG GGTGTTTATT GGGCCTCAGA ACTGCAGCTG TGGGCGTGGA 
ACATTTTGTA TTCATCTGTT ATTCGTAATG CTCCGAGTAT TTCAACTAGA ACCTTCGGAC 
CCAATGTTAT GGAGAAAAAC TTTAAAGAAT TTTGAGGTTG AGAGTTTGTT CCAGAAATAT 
CACAGTAGGC GTAGCTCAAG GATCAAAGCT CCATCTCGTA ACACCATCCA GAAGTTTGTT 
TCACGCATGT CAAATTCTCA TACATTGTCA TCATCTAGTA CTTCTACATC TAGTTCAGAA 
AACAGTATAA AAGATGAAGA GGAACAGATG TGTCCTATTT GCTTGTTGGG CATGCTTGAT 
GAAGAAAGTC TTACAGTGTG TGAAGATGGC TGCAGGAACA AGCTGCACCA CCACTGCATG 
TCTATTTGGG CAGAAGAATG TAGAAGAAAT AAAGAACCTT TAATATGTCC CCTTTGTAGA 
TCTAAGTGGA GGTCACATGA TTTCTACAGT CACGAGCTGT CAAGCCCTGT GGATTCCCCT 
TCTTCTCTGC GAGCTGCACA GCAGCAAACC ATACAGCAGC CACCTCTGGC TGGACCACAA 
CGAAGGAATC AGGAGAACAA TTTTAACCTT ACTCATTATG GAACTCAGCA GATTCCTCCT 
GCTTATAAAG ATTTAGCTGA GCCATGGATT CAGGTATTTG GAATGGAACT CGTTGGCTGC 
TTATTTTCTA GAAACTGGAA TGTAAGAGAG ATGGCTCTCA GACGTCTTTC CCACGATGTT 
AGTGGGGCTC TGCTATTGGC AAATGGGGAG AGCACTGGAA ATTCCGGGAG CAATGGTGGA 
AACAGCCCGA GTGCCGGAGC TGCCAGTGGG TCTTCCCAGA CCAGTATCTC AGGAAATGTG 
GTTGAGGCGT GCTGCAACGT TTTGTCAATG GTCTGTGCTG ACCCTGTCTA CAAAGTGTAC 
GTTGCTGCTT TAAAAACGTT GAGAGCCATG CTAGTATATA CTCCTTGCCA TAGTTTGGCA 
GAAAGAATCA AACTGCAGAG ACTTCTCCGG CCAGTTGTAG ATACCATCCT AGTCAAGTGC 
GCAGATGCCA ATAGTCGTAC AAGTCAGCTG TCTATATCAA CACTGTTGGA ATTATGCAAA 
GGTCAAGCAG GAGAGTTGGC AGTTGGCAGA GAAATACTAA AAGCTGGATC CATTGGTATT 
GGTGGTGTTG ATTATGTCTT AAATTGTATT CTTGGAAACC AAACTGAATC AAACAACTGG 
CAAGAACTCC TGGGCCGCCT TTGTCTTATA GATAGACTGC TGTTGGAATT TCCTGCTGAA 
TTTTACCCTC ATATTGTCAG TACAGATGTT TCACAAGCTG AGCCTGTTGA AGTCAGGTAT 
AAGAAGCTGC TGTCCCTCTT AACCTTTGCT TTGCAGTCCA TTGATAATTC CCACTCGATG 
GTTGGTAAAC TCTCCCGAAG GATATATTTG AGTTCTGCAA GAATGGTTAC TGCCGTACCC 
CACGTATTTT CAAAACTGTT AGAAATGCTG AATGTGTCCA GTTCCACTCA TTTTACCAGG 
ATGCGTCGTC GTTTGATGGC TATTGCAGAT GAGGTGGAAA TCGCTGAGGC CATCCAGCTG 
GGCATAGAAG ACACTTTGGA TGGTCGACAT GACAGCTTTT TGCAGGCATC TGTCCTTAAC 
AGCTATCCAG AAACCACAGA GAATAGTTCC CTTGGGTGCA CAGACCATTT AGAGAAAACT 
GGAAAAAGAT CGTGTGCTAC AAAATTGAGT GCCAGTTCGG AGGACATTTC TGACAGACTG 
GCCAGCATTT CGGTAGGACT TCCTAGTTCA ACAGCAACGA TGGAGCAACC AAAGCCAATG 
GTTCAGACAA AAGGCAGACC CCACAGTCAG TGTTTGAACT CATCTCTTTT ATCTCATCAT 
TCCCAATTAA TGTTCCCAGC CTTGTCAACC CCTTCTTCAT CTGCCCCAGC TGTACCAGCT 
GGCACCACAA CAGATGTCTC TAAGCATAGA CCTCAGGGAT TCATTCCCTG CAAAATACCT 
TCTGCATCTC CTCAAACACA GCGCAAGTTT TCTCTACAGT TCCAGAGAAA CTGTTCTGAA 
AACAAAGACT CAGATAAACT TTCCCCAGTC TTTACTCAGT CAAGACCCCT GCCCTCCAGT 
AATATACACA GGCCAAAGCC ATCTCGACCT ACCCCAGGTA ATACAAGTAA ACAGGGAGAT 
GACTCAAAAA ATAGCATGAC ACTTGATTTA AACAGTACTT CCAAATGTGA TGACAGCTTT 
GGCAGTAGCA GCAACAGTAG TAATGCTGTG ATACCTAGTG ACGAGACAGT GTTCACCCCA 
GTGGAGGAGA AATGCAGATT AGATGTCAAT ACAGAACTCA ACTCTAGTAT TGAGGACCTT 
CTTGAAGCGT CTATGCCCTC AAATGACACA ACAGTCACTT TTAAGTCAGA AGTTGCTGTC 
CTCTCTCCTG AAAAGGATGA AAATGATGAT ACCTACAAAG ATGATGTGAA TCATAATCAA 
AAGTGCAAAG AAAAGATGGA AGCTGAAGAA GAAGAAGCGT TAGCAATTGC CATGGCAATG 
TCAGCATCTC AGGATGCCCT CCCCATAGTT CCTCAACTAC AGGTTGAAAA TGGAGAAGAT 
ATCATCATTA TTCAACAGGA TACACCAGAG ACTCTGCCAG GACATACCAA AGCAAAACAA 
CCTTACAGAG AAGACACTGA ATGGCTGAAA GGTCAGCAGA TAGGTCTTGG AGCATTTTCT 
TCTTGTTACC AGGCTCAAGA TGTGGGAACT GGAACTTTAA TGGCTGTTAA ACAGGTGACG 
TATGTCAGAA ATACATCATC TGAGCAAGAA GAAGTAGTGG AAGCATTAAG AGAAGAGATA 
AGAATGATGA GCCATCTGAA TCATCCAAAT ATCATTAGGA TGTTGGGAGC CACATGTGAG 
AAGAGCAATT ATAATCTCTT CATCGAATGG ATGGCAGGGG GTTCTGTGGC TCATTTGCTC 
AGTAAATATG GAGCCTTCAA GGAATCAGTA GTTATTAACT ATACTGAACA ATTACTTCGT 
GGCCTTTCAT ATCTGCATGA AAACCAGATC ATTCACAGAG ATGTCAAAGG TGCCAATTTG 
CTAATTGACA GCACAGGTCA GAGACTGAGA ATTGCAGATT TTGGAGCTGC AGCCAGGTTG 
GCATCAAAAG GAACTGGTGC AGGAGAGTTT CAGGGACAGT TATTGGGGAC AATTGCATTT 
ATGGCACCTG AGGTACTCAG AGGTCAGCAG TATGGTAGGA GCTGTGATGT GTGGAGTGTT 
GGCTGTGCTA TTATAGAAAT GGCTTGTGCT AAACCACCTT GGAATGCAGA AAAACATTCC 
AATCATCTTG CTTTGATATT CAAGATTGCT AGTGCAACTA CTGCTCCATC CATCCCTTCA 
CATTTGTCTC CCGGTTTACG AGATGTGGCT CTTCGTTGTT TAGAACTTCA ACCTCAGGAC 
AGACCTCCGT CGAGAGAGCT ACTGAAGCAT CCGGTCTTCC GTACTACATG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023392966.1
CDS	282..4535
Translation	MCQPFSFKAVVTAPRSLSGVAGDRGAGAVLPAPCSCAGCPRTAGFQGPQCKWASAQIVLCCLCSTVCVSREMENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNKRGPVVVKPIPIKGDGSEINNLATESQGEGQASTTSPAPKGRRSPSPGSSPSGRTVKPDSPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNKEPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTIQQPPLAGPQRRNQENNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGSNGGNSPSAGAASGSSQTSISGNVVEACCNVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLNCILGNQTESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEVRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPHVFSKLLEMLNVSSSTHFTRMRRRLMAIADEVEIAEAIQLGIEDTLDGRHDSFLQASVLNSYPETTENSSLGCTDHLEKTGKRSCATKLSASSEDISDRLASISVGLPSSTATMEQPKPMVQTKGRPHSQCLNSSLLSHHSQLMFPALSTPSSSAPAVPAGTTTDVSKHRPQGFIPCKIPSASPQTQRKFSLQFQRNCSENKDSDKLSPVFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDDSKNSMTLDLNSTSKCDDSFGSSSNSSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSNDTTVTFKSEVAVLSPEKDENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_023537198.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023537198.1

ORF Insert Sequence:

ATGTGTCAGC CATTCAGTTT CAAAGCGGTG GTGACTGCTC CCCGCAGCCT CTCCGGCGTG 
GCTGGAGACC GTGGCGCGGG TGCGGTGCTT CCTGCTCCGT GCAGCTGCGC GGGCTGCCCG 
CGGACTGCCG GATTCCAGGG GCCACAGTGC AAGTGGGCTT CGGCTCAGAT AGTTCTGTGC 
TGCCTGTGCA GTACGGTATG TGTAAGTCGT GAGATGGAGA ATAAGGAAAC TCTCAAGGGT 
TTGCACAAGA TGGATGATCG CCCAGAAGAA CGAATGATCA GGGAGAAACT GAAGGCAACC 
TGTATGCCAG CCTGGAAGCA CGAATGGCTG GAAAGGAGAA ATAAGAGAGG CCCTGTGGTG 
GTAAAACCAA TCCCTATTAA AGGAGATGGA TCTGAAATTA ATAACTTGGC AACTGAGTCT 
CAAGGAGAGG GCCAGGCGAG TACCACTTCA CCAGCTCCCA AAGGCCGACG CAGTCCTTCC 
CCTGGCAGCT CCCCATCAGG TCGCACAGTG AAACCAGACT CTCCAGGAGT AAGGAGAAAA 
AGAGTTTCCC CCGTGCCATT TCAGAGTGGG AGAATCACAC CACCTCGAAG AGCCCCTTCA 
CCAGATGGCT TCTCACCATA CAGCCCTGAG GAAACAAACC GCCGTGTAAA CAAAGTGATG 
CGGGCCAGGC TGTACTTACT GCAGCAAATA GGACCCAACT CTTTCCTGAT TGGAGGAGAT 
AGTCCAGACA ATAAATACCG GGTGTTTATT GGGCCTCAGA ACTGCAGCTG TGGGCGTGGA 
ACATTTTGTA TTCATCTGTT ATTCGTAATG CTCCGAGTAT TTCAACTAGA ACCTTCGGAC 
CCAATGTTAT GGAGAAAAAC TTTAAAGAAT TTTGAGGTTG AGAGTTTGTT CCAGAAATAT 
CACAGTAGGC GTAGCTCAAG GATCAAAGCT CCATCTCGTA ACACCATCCA GAAGTTTGTT 
TCACGCATGT CAAATTCTCA TACATTGTCA TCATCTAGTA CTTCTACATC TAGTTCAGAA 
AACAGTATAA AAGATGAAGA GGAACAGATG TGTCCTATTT GCTTGTTGGG CATGCTTGAT 
GAAGAAAGTC TTACAGTGTG TGAAGATGGC TGCAGGAACA AGCTGCACCA CCACTGCATG 
TCTATTTGGG CAGAAGAATG TAGAAGAAAT AAAGAACCTT TAATATGTCC CCTTTGTAGA 
TCTAAGTGGA GGTCACATGA TTTCTACAGT CACGAGCTGT CAAGCCCTGT GGATTCCCCT 
TCTTCTCTGC GAGCTGCACA GCAGCAAACC ATACAGCAGC CACCTCTGGC TGGACCACAA 
CGAAGGAATC AGGAGAACAA TTTTAACCTT ACTCATTATG GAACTCAGCA GATTCCTCCT 
GCTTATAAAG ATTTAGCTGA GCCATGGATT CAGGTATTTG GAATGGAACT CGTTGGCTGC 
TTATTTTCTA GAAACTGGAA TGTAAGAGAG ATGGCTCTCA GACGTCTTTC CCACGATGTT 
AGTGGGGCTC TGCTATTGGC AAATGGGGAG AGCACTGGAA ATTCCGGGAG CAATGGTGGA 
AACAGCCCGA GTGCCGGAGC TGCCAGTGGG TCTTCCCAGA CCAGTATCTC AGGAAATGTG 
GTTGAGGCGT GCTGCAACGT TTTGTCAATG GTCTGTGCTG ACCCTGTCTA CAAAGTGTAC 
GTTGCTGCTT TAAAAACGTT GAGAGCCATG CTAGTATATA CTCCTTGCCA TAGTTTGGCA 
GAAAGAATCA AACTGCAGAG ACTTCTCCGG CCAGTTGTAG ATACCATCCT AGTCAAGTGC 
GCAGATGCCA ATAGTCGTAC AAGTCAGCTG TCTATATCAA CACTGTTGGA ATTATGCAAA 
GGTCAAGCAG GAGAGTTGGC AGTTGGCAGA GAAATACTAA AAGCTGGATC CATTGGTATT 
GGTGGTGTTG ATTATGTCTT AAATTGTATT CTTGGAAACC AAACTGAATC AAACAACTGG 
CAAGAACTCC TGGGCCGCCT TTGTCTTATA GATAGACTGC TGTTGGAATT TCCTGCTGAA 
TTTTACCCTC ATATTGTCAG TACAGATGTT TCACAAGCTG AGCCTGTTGA AGTCAGGTAT 
AAGAAGCTGC TGTCCCTCTT AACCTTTGCT TTGCAGTCCA TTGATAATTC CCACTCGATG 
GTTGGTAAAC TCTCCCGAAG GATATATTTG AGTTCTGCAA GAATGGTTAC TGCCGTACCC 
CACGTATTTT CAAAACTGTT AGAAATGCTG AATGTGTCCA GTTCCACTCA TTTTACCAGG 
ATGCGTCGTC GTTTGATGGC TATTGCAGAT GAGGTGGAAA TCGCTGAGGC CATCCAGCTG 
GGCATAGAAG ACACTTTGGA TGGTCGACAT GACAGCTTTT TGCAGGCATC TGTCCTTAAC 
AGCTATCCAG AAACCACAGA GAATAGTTCC CTTGGGTGCA CAGACCATTT AGAGAAAACT 
GGAAAAAGAT CGTGTGCTAC AAAATTGAGT GCCAGTTCGG AGGACATTTC TGACAGACTG 
GCCAGCATTT CGGTAGGACT TCCTAGTTCA ACAGCAACGA TGGAGCAACC AAAGCCAATG 
GTTCAGACAA AAGGCAGACC CCACAGTCAG TGTTTGAACT CATCTCTTTT ATCTCATCAT 
TCCCAATTAA TGTTCCCAGC CTTGTCAACC CCTTCTTCAT CTGCCCCAGC TGTACCAGCT 
GGCACCACAA CAGATGTCTC TAAGCATAGA CCTCAGGGAT TCATTCCCTG CAAAATACCT 
TCTGCATCTC CTCAAACACA GCGCAAGTTT TCTCTACAGT TCCAGAGAAA CTGTTCTGAA 
AACAAAGACT CAGATAAACT TTCCCCAGTC TTTACTCAGT CAAGACCCCT GCCCTCCAGT 
AATATACACA GGCCAAAGCC ATCTCGACCT ACCCCAGGTA ATACAAGTAA ACAGGGAGAT 
GACTCAAAAA ATAGCATGAC ACTTGATTTA AACAGTACTT CCAAATGTGA TGACAGCTTT 
GGCAGTAGCA GCAACAGTAG TAATGCTGTG ATACCTAGTG ACGAGACAGT GTTCACCCCA 
GTGGAGGAGA AATGCAGATT AGATGTCAAT ACAGAACTCA ACTCTAGTAT TGAGGACCTT 
CTTGAAGCGT CTATGCCCTC AAATGACACA ACAGTCACTT TTAAGTCAGA AGTTGCTGTC 
CTCTCTCCTG AAAAGGATGA AAATGATGAT ACCTACAAAG ATGATGTGAA TCATAATCAA 
AAGTGCAAAG AAAAGATGGA AGCTGAAGAA GAAGAAGCGT TAGCAATTGC CATGGCAATG 
TCAGCATCTC AGGATGCCCT CCCCATAGTT CCTCAACTAC AGGTTGAAAA TGGAGAAGAT 
ATCATCATTA TTCAACAGGA TACACCAGAG ACTCTGCCAG GACATACCAA AGCAAAACAA 
CCTTACAGAG AAGACACTGA ATGGCTGAAA GGTCAGCAGA TAGGTCTTGG AGCATTTTCT 
TCTTGTTACC AGGCTCAAGA TGTGGGAACT GGAACTTTAA TGGCTGTTAA ACAGGTGACG 
TATGTCAGAA ATACATCATC TGAGCAAGAA GAAGTAGTGG AAGCATTAAG AGAAGAGATA 
AGAATGATGA GCCATCTGAA TCATCCAAAT ATCATTAGGA TGTTGGGAGC CACATGTGAG 
AAGAGCAATT ATAATCTCTT CATCGAATGG ATGGCAGGGG GTTCTGTGGC TCATTTGCTC 
AGTAAATATG GAGCCTTCAA GGAATCAGTA GTTATTAACT ATACTGAACA ATTACTTCGT 
GGCCTTTCAT ATCTGCATGA AAACCAGATC ATTCACAGAG ATGTCAAAGG TGCCAATTTG 
CTAATTGACA GCACAGGTCA GAGACTGAGA ATTGCAGATT TTGGAGCTGC AGCCAGGTTG 
GCATCAAAAG GAACTGGTGC AGGAGAGTTT CAGGGACAGT TATTGGGGAC AATTGCATTT 
ATGGCACCTG AGGTACTCAG AGGTCAGCAG TATGGTAGGA GCTGTGATGT GTGGAGTGTT 
GGCTGTGCTA TTATAGAAAT GGCTTGTGCT AAACCACCTT GGAATGCAGA AAAACATTCC 
AATCATCTTG CTTTGATATT CAAGATTGCT AGTGCAACTA CTGCTCCATC CATCCCTTCA 
CATTTGTCTC CCGGTTTACG AGATGTGGCT CTTCGTTGTT TAGAACTTCA ACCTCAGGAC 
AGACCTCCGT CGAGAGAGCT ACTGAAGCAT CCGGTCTTCC GTACTACATG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_023537199.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023392967.1 Pteropus vampyrus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPt73639
Clone ID Related Accession (Same CDS sequence)	XM_023537199.1
Accession Version	XM_023537199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888962.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA AGGAAACTCT CAAGGGTTTG CACAAGATGG ATGATCGCCC AGAAGAACGA 
ATGATCAGGG AGAAACTGAA GGCAACCTGT ATGCCAGCCT GGAAGCACGA ATGGCTGGAA 
AGGAGAAATA AGAGAGGCCC TGTGGTGGTA AAACCAATCC CTATTAAAGG AGATGGATCT 
GAAATTAATA ACTTGGCAAC TGAGTCTCAA GGAGAGGGCC AGGCGAGTAC CACTTCACCA 
GCTCCCAAAG GCCGACGCAG TCCTTCCCCT GGCAGCTCCC CATCAGGTCG CACAGTGAAA 
CCAGACTCTC CAGGAGTAAG GAGAAAAAGA GTTTCCCCCG TGCCATTTCA GAGTGGGAGA 
ATCACACCAC CTCGAAGAGC CCCTTCACCA GATGGCTTCT CACCATACAG CCCTGAGGAA 
ACAAACCGCC GTGTAAACAA AGTGATGCGG GCCAGGCTGT ACTTACTGCA GCAAATAGGA 
CCCAACTCTT TCCTGATTGG AGGAGATAGT CCAGACAATA AATACCGGGT GTTTATTGGG 
CCTCAGAACT GCAGCTGTGG GCGTGGAACA TTTTGTATTC ATCTGTTATT CGTAATGCTC 
CGAGTATTTC AACTAGAACC TTCGGACCCA ATGTTATGGA GAAAAACTTT AAAGAATTTT 
GAGGTTGAGA GTTTGTTCCA GAAATATCAC AGTAGGCGTA GCTCAAGGAT CAAAGCTCCA 
TCTCGTAACA CCATCCAGAA GTTTGTTTCA CGCATGTCAA ATTCTCATAC ATTGTCATCA 
TCTAGTACTT CTACATCTAG TTCAGAAAAC AGTATAAAAG ATGAAGAGGA ACAGATGTGT 
CCTATTTGCT TGTTGGGCAT GCTTGATGAA GAAAGTCTTA CAGTGTGTGA AGATGGCTGC 
AGGAACAAGC TGCACCACCA CTGCATGTCT ATTTGGGCAG AAGAATGTAG AAGAAATAAA 
GAACCTTTAA TATGTCCCCT TTGTAGATCT AAGTGGAGGT CACATGATTT CTACAGTCAC 
GAGCTGTCAA GCCCTGTGGA TTCCCCTTCT TCTCTGCGAG CTGCACAGCA GCAAACCATA 
CAGCAGCCAC CTCTGGCTGG ACCACAACGA AGGAATCAGG AGAACAATTT TAACCTTACT 
CATTATGGAA CTCAGCAGAT TCCTCCTGCT TATAAAGATT TAGCTGAGCC ATGGATTCAG 
GTATTTGGAA TGGAACTCGT TGGCTGCTTA TTTTCTAGAA ACTGGAATGT AAGAGAGATG 
GCTCTCAGAC GTCTTTCCCA CGATGTTAGT GGGGCTCTGC TATTGGCAAA TGGGGAGAGC 
ACTGGAAATT CCGGGAGCAA TGGTGGAAAC AGCCCGAGTG CCGGAGCTGC CAGTGGGTCT 
TCCCAGACCA GTATCTCAGG AAATGTGGTT GAGGCGTGCT GCAACGTTTT GTCAATGGTC 
TGTGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACGTTGAG AGCCATGCTA 
GTATATACTC CTTGCCATAG TTTGGCAGAA AGAATCAAAC TGCAGAGACT TCTCCGGCCA 
GTTGTAGATA CCATCCTAGT CAAGTGCGCA GATGCCAATA GTCGTACAAG TCAGCTGTCT 
ATATCAACAC TGTTGGAATT ATGCAAAGGT CAAGCAGGAG AGTTGGCAGT TGGCAGAGAA 
ATACTAAAAG CTGGATCCAT TGGTATTGGT GGTGTTGATT ATGTCTTAAA TTGTATTCTT 
GGAAACCAAA CTGAATCAAA CAACTGGCAA GAACTCCTGG GCCGCCTTTG TCTTATAGAT 
AGACTGCTGT TGGAATTTCC TGCTGAATTT TACCCTCATA TTGTCAGTAC AGATGTTTCA 
CAAGCTGAGC CTGTTGAAGT CAGGTATAAG AAGCTGCTGT CCCTCTTAAC CTTTGCTTTG 
CAGTCCATTG ATAATTCCCA CTCGATGGTT GGTAAACTCT CCCGAAGGAT ATATTTGAGT 
TCTGCAAGAA TGGTTACTGC CGTACCCCAC GTATTTTCAA AACTGTTAGA AATGCTGAAT 
GTGTCCAGTT CCACTCATTT TACCAGGATG CGTCGTCGTT TGATGGCTAT TGCAGATGAG 
GTGGAAATCG CTGAGGCCAT CCAGCTGGGC ATAGAAGACA CTTTGGATGG TCGACATGAC 
AGCTTTTTGC AGGCATCTGT CCTTAACAGC TATCCAGAAA CCACAGAGAA TAGTTCCCTT 
GGGTGCACAG ACCATTTAGA GAAAACTGGA AAAAGATCGT GTGCTACAAA ATTGAGTGCC 
AGTTCGGAGG ACATTTCTGA CAGACTGGCC AGCATTTCGG TAGGACTTCC TAGTTCAACA 
GCAACGATGG AGCAACCAAA GCCAATGGTT CAGACAAAAG GCAGACCCCA CAGTCAGTGT 
TTGAACTCAT CTCTTTTATC TCATCATTCC CAATTAATGT TCCCAGCCTT GTCAACCCCT 
TCTTCATCTG CCCCAGCTGT ACCAGCTGGC ACCACAACAG ATGTCTCTAA GCATAGACCT 
CAGGGATTCA TTCCCTGCAA AATACCTTCT GCATCTCCTC AAACACAGCG CAAGTTTTCT 
CTACAGTTCC AGAGAAACTG TTCTGAAAAC AAAGACTCAG ATAAACTTTC CCCAGTCTTT 
ACTCAGTCAA GACCCCTGCC CTCCAGTAAT ATACACAGGC CAAAGCCATC TCGACCTACC 
CCAGGTAATA CAAGTAAACA GGGAGATGAC TCAAAAAATA GCATGACACT TGATTTAAAC 
AGTACTTCCA AATGTGATGA CAGCTTTGGC AGTAGCAGCA ACAGTAGTAA TGCTGTGATA 
CCTAGTGACG AGACAGTGTT CACCCCAGTG GAGGAGAAAT GCAGATTAGA TGTCAATACA 
GAACTCAACT CTAGTATTGA GGACCTTCTT GAAGCGTCTA TGCCCTCAAA TGACACAACA 
GTCACTTTTA AGTCAGAAGT TGCTGTCCTC TCTCCTGAAA AGGATGAAAA TGATGATACC 
TACAAAGATG ATGTGAATCA TAATCAAAAG TGCAAAGAAA AGATGGAAGC TGAAGAAGAA 
GAAGCGTTAG CAATTGCCAT GGCAATGTCA GCATCTCAGG ATGCCCTCCC CATAGTTCCT 
CAACTACAGG TTGAAAATGG AGAAGATATC ATCATTATTC AACAGGATAC ACCAGAGACT 
CTGCCAGGAC ATACCAAAGC AAAACAACCT TACAGAGAAG ACACTGAATG GCTGAAAGGT 
CAGCAGATAG GTCTTGGAGC ATTTTCTTCT TGTTACCAGG CTCAAGATGT GGGAACTGGA 
ACTTTAATGG CTGTTAAACA GGTGACGTAT GTCAGAAATA CATCATCTGA GCAAGAAGAA 
GTAGTGGAAG CATTAAGAGA AGAGATAAGA ATGATGAGCC ATCTGAATCA TCCAAATATC 
ATTAGGATGT TGGGAGCCAC ATGTGAGAAG AGCAATTATA ATCTCTTCAT CGAATGGATG 
GCAGGGGGTT CTGTGGCTCA TTTGCTCAGT AAATATGGAG CCTTCAAGGA ATCAGTAGTT 
ATTAACTATA CTGAACAATT ACTTCGTGGC CTTTCATATC TGCATGAAAA CCAGATCATT 
CACAGAGATG TCAAAGGTGC CAATTTGCTA ATTGACAGCA CAGGTCAGAG ACTGAGAATT 
GCAGATTTTG GAGCTGCAGC CAGGTTGGCA TCAAAAGGAA CTGGTGCAGG AGAGTTTCAG 
GGACAGTTAT TGGGGACAAT TGCATTTATG GCACCTGAGG TACTCAGAGG TCAGCAGTAT 
GGTAGGAGCT GTGATGTGTG GAGTGTTGGC TGTGCTATTA TAGAAATGGC TTGTGCTAAA 
CCACCTTGGA ATGCAGAAAA ACATTCCAAT CATCTTGCTT TGATATTCAA GATTGCTAGT 
GCAACTACTG CTCCATCCAT CCCTTCACAT TTGTCTCCCG GTTTACGAGA TGTGGCTCTT 
CGTTGTTTAG AACTTCAACC TCAGGACAGA CCTCCGTCGA GAGAGCTACT GAAGCATCCG 
GTCTTCCGTA CTACATGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023392967.1
CDS	757..4797
Translation	MENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNKRGPVVVKPIPIKGDGSEINNLATESQGEGQASTTSPAPKGRRSPSPGSSPSGRTVKPDSPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNKEPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTIQQPPLAGPQRRNQENNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGSNGGNSPSAGAASGSSQTSISGNVVEACCNVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLNCILGNQTESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEVRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPHVFSKLLEMLNVSSSTHFTRMRRRLMAIADEVEIAEAIQLGIEDTLDGRHDSFLQASVLNSYPETTENSSLGCTDHLEKTGKRSCATKLSASSEDISDRLASISVGLPSSTATMEQPKPMVQTKGRPHSQCLNSSLLSHHSQLMFPALSTPSSSAPAVPAGTTTDVSKHRPQGFIPCKIPSASPQTQRKFSLQFQRNCSENKDSDKLSPVFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDDSKNSMTLDLNSTSKCDDSFGSSSNSSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSNDTTVTFKSEVAVLSPEKDENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_023537199.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023537199.1

ORF Insert Sequence:

ATGGAGAATA AGGAAACTCT CAAGGGTTTG CACAAGATGG ATGATCGCCC AGAAGAACGA 
ATGATCAGGG AGAAACTGAA GGCAACCTGT ATGCCAGCCT GGAAGCACGA ATGGCTGGAA 
AGGAGAAATA AGAGAGGCCC TGTGGTGGTA AAACCAATCC CTATTAAAGG AGATGGATCT 
GAAATTAATA ACTTGGCAAC TGAGTCTCAA GGAGAGGGCC AGGCGAGTAC CACTTCACCA 
GCTCCCAAAG GCCGACGCAG TCCTTCCCCT GGCAGCTCCC CATCAGGTCG CACAGTGAAA 
CCAGACTCTC CAGGAGTAAG GAGAAAAAGA GTTTCCCCCG TGCCATTTCA GAGTGGGAGA 
ATCACACCAC CTCGAAGAGC CCCTTCACCA GATGGCTTCT CACCATACAG CCCTGAGGAA 
ACAAACCGCC GTGTAAACAA AGTGATGCGG GCCAGGCTGT ACTTACTGCA GCAAATAGGA 
CCCAACTCTT TCCTGATTGG AGGAGATAGT CCAGACAATA AATACCGGGT GTTTATTGGG 
CCTCAGAACT GCAGCTGTGG GCGTGGAACA TTTTGTATTC ATCTGTTATT CGTAATGCTC 
CGAGTATTTC AACTAGAACC TTCGGACCCA ATGTTATGGA GAAAAACTTT AAAGAATTTT 
GAGGTTGAGA GTTTGTTCCA GAAATATCAC AGTAGGCGTA GCTCAAGGAT CAAAGCTCCA 
TCTCGTAACA CCATCCAGAA GTTTGTTTCA CGCATGTCAA ATTCTCATAC ATTGTCATCA 
TCTAGTACTT CTACATCTAG TTCAGAAAAC AGTATAAAAG ATGAAGAGGA ACAGATGTGT 
CCTATTTGCT TGTTGGGCAT GCTTGATGAA GAAAGTCTTA CAGTGTGTGA AGATGGCTGC 
AGGAACAAGC TGCACCACCA CTGCATGTCT ATTTGGGCAG AAGAATGTAG AAGAAATAAA 
GAACCTTTAA TATGTCCCCT TTGTAGATCT AAGTGGAGGT CACATGATTT CTACAGTCAC 
GAGCTGTCAA GCCCTGTGGA TTCCCCTTCT TCTCTGCGAG CTGCACAGCA GCAAACCATA 
CAGCAGCCAC CTCTGGCTGG ACCACAACGA AGGAATCAGG AGAACAATTT TAACCTTACT 
CATTATGGAA CTCAGCAGAT TCCTCCTGCT TATAAAGATT TAGCTGAGCC ATGGATTCAG 
GTATTTGGAA TGGAACTCGT TGGCTGCTTA TTTTCTAGAA ACTGGAATGT AAGAGAGATG 
GCTCTCAGAC GTCTTTCCCA CGATGTTAGT GGGGCTCTGC TATTGGCAAA TGGGGAGAGC 
ACTGGAAATT CCGGGAGCAA TGGTGGAAAC AGCCCGAGTG CCGGAGCTGC CAGTGGGTCT 
TCCCAGACCA GTATCTCAGG AAATGTGGTT GAGGCGTGCT GCAACGTTTT GTCAATGGTC 
TGTGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACGTTGAG AGCCATGCTA 
GTATATACTC CTTGCCATAG TTTGGCAGAA AGAATCAAAC TGCAGAGACT TCTCCGGCCA 
GTTGTAGATA CCATCCTAGT CAAGTGCGCA GATGCCAATA GTCGTACAAG TCAGCTGTCT 
ATATCAACAC TGTTGGAATT ATGCAAAGGT CAAGCAGGAG AGTTGGCAGT TGGCAGAGAA 
ATACTAAAAG CTGGATCCAT TGGTATTGGT GGTGTTGATT ATGTCTTAAA TTGTATTCTT 
GGAAACCAAA CTGAATCAAA CAACTGGCAA GAACTCCTGG GCCGCCTTTG TCTTATAGAT 
AGACTGCTGT TGGAATTTCC TGCTGAATTT TACCCTCATA TTGTCAGTAC AGATGTTTCA 
CAAGCTGAGC CTGTTGAAGT CAGGTATAAG AAGCTGCTGT CCCTCTTAAC CTTTGCTTTG 
CAGTCCATTG ATAATTCCCA CTCGATGGTT GGTAAACTCT CCCGAAGGAT ATATTTGAGT 
TCTGCAAGAA TGGTTACTGC CGTACCCCAC GTATTTTCAA AACTGTTAGA AATGCTGAAT 
GTGTCCAGTT CCACTCATTT TACCAGGATG CGTCGTCGTT TGATGGCTAT TGCAGATGAG 
GTGGAAATCG CTGAGGCCAT CCAGCTGGGC ATAGAAGACA CTTTGGATGG TCGACATGAC 
AGCTTTTTGC AGGCATCTGT CCTTAACAGC TATCCAGAAA CCACAGAGAA TAGTTCCCTT 
GGGTGCACAG ACCATTTAGA GAAAACTGGA AAAAGATCGT GTGCTACAAA ATTGAGTGCC 
AGTTCGGAGG ACATTTCTGA CAGACTGGCC AGCATTTCGG TAGGACTTCC TAGTTCAACA 
GCAACGATGG AGCAACCAAA GCCAATGGTT CAGACAAAAG GCAGACCCCA CAGTCAGTGT 
TTGAACTCAT CTCTTTTATC TCATCATTCC CAATTAATGT TCCCAGCCTT GTCAACCCCT 
TCTTCATCTG CCCCAGCTGT ACCAGCTGGC ACCACAACAG ATGTCTCTAA GCATAGACCT 
CAGGGATTCA TTCCCTGCAA AATACCTTCT GCATCTCCTC AAACACAGCG CAAGTTTTCT 
CTACAGTTCC AGAGAAACTG TTCTGAAAAC AAAGACTCAG ATAAACTTTC CCCAGTCTTT 
ACTCAGTCAA GACCCCTGCC CTCCAGTAAT ATACACAGGC CAAAGCCATC TCGACCTACC 
CCAGGTAATA CAAGTAAACA GGGAGATGAC TCAAAAAATA GCATGACACT TGATTTAAAC 
AGTACTTCCA AATGTGATGA CAGCTTTGGC AGTAGCAGCA ACAGTAGTAA TGCTGTGATA 
CCTAGTGACG AGACAGTGTT CACCCCAGTG GAGGAGAAAT GCAGATTAGA TGTCAATACA 
GAACTCAACT CTAGTATTGA GGACCTTCTT GAAGCGTCTA TGCCCTCAAA TGACACAACA 
GTCACTTTTA AGTCAGAAGT TGCTGTCCTC TCTCCTGAAA AGGATGAAAA TGATGATACC 
TACAAAGATG ATGTGAATCA TAATCAAAAG TGCAAAGAAA AGATGGAAGC TGAAGAAGAA 
GAAGCGTTAG CAATTGCCAT GGCAATGTCA GCATCTCAGG ATGCCCTCCC CATAGTTCCT 
CAACTACAGG TTGAAAATGG AGAAGATATC ATCATTATTC AACAGGATAC ACCAGAGACT 
CTGCCAGGAC ATACCAAAGC AAAACAACCT TACAGAGAAG ACACTGAATG GCTGAAAGGT 
CAGCAGATAG GTCTTGGAGC ATTTTCTTCT TGTTACCAGG CTCAAGATGT GGGAACTGGA 
ACTTTAATGG CTGTTAAACA GGTGACGTAT GTCAGAAATA CATCATCTGA GCAAGAAGAA 
GTAGTGGAAG CATTAAGAGA AGAGATAAGA ATGATGAGCC ATCTGAATCA TCCAAATATC 
ATTAGGATGT TGGGAGCCAC ATGTGAGAAG AGCAATTATA ATCTCTTCAT CGAATGGATG 
GCAGGGGGTT CTGTGGCTCA TTTGCTCAGT AAATATGGAG CCTTCAAGGA ATCAGTAGTT 
ATTAACTATA CTGAACAATT ACTTCGTGGC CTTTCATATC TGCATGAAAA CCAGATCATT 
CACAGAGATG TCAAAGGTGC CAATTTGCTA ATTGACAGCA CAGGTCAGAG ACTGAGAATT 
GCAGATTTTG GAGCTGCAGC CAGGTTGGCA TCAAAAGGAA CTGGTGCAGG AGAGTTTCAG 
GGACAGTTAT TGGGGACAAT TGCATTTATG GCACCTGAGG TACTCAGAGG TCAGCAGTAT 
GGTAGGAGCT GTGATGTGTG GAGTGTTGGC TGTGCTATTA TAGAAATGGC TTGTGCTAAA 
CCACCTTGGA ATGCAGAAAA ACATTCCAAT CATCTTGCTT TGATATTCAA GATTGCTAGT 
GCAACTACTG CTCCATCCAT CCCTTCACAT TTGTCTCCCG GTTTACGAGA TGTGGCTCTT 
CGTTGTTTAG AACTTCAACC TCAGGACAGA CCTCCGTCGA GAGAGCTACT GAAGCATCCG 
GTCTTCCGTA CTACATGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_011371048.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011369350.1 Pteropus vampyrus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), mRNA.

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CloneID	OPt54651
Clone ID Related Accession (Same CDS sequence)	XM_011371048.1 , XM_011371048.2
Accession Version	XM_011371048.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	mitogen-activated protein kinase kinase kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888962.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGTGC CGGGGCGGCA 
ACCCCCGAGG CGGGCGGCGG CGGAGGACCG CTTAAGGCGA GCAGCGTGCC TGGGGCGACT 
GCGGGGGTTC TGCGGGAGGC GGGCAGCGGG GGCCGCGAGC GGGCGGACTG GAGGCGGCGG 
CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC 
GCCGCGTCGC CGCTGTCCTC CTCCACTTCC CCGTCGCCGG AGCCTGCAGA CCCGGCGGCG 
GACGGGAGCG GTTTCCAGCC TGTGGCGATG CCGCAGCCTC ACGGAGCCGC GAGCCGCTGC 
GGCGCCCAAC CTCCCGAGTC GGCGGCCGCA CCGGACAGCG GCGCCCCAAG CCCCGTGGGG 
GCCGATCCCA GAGAGAAGCG GACGCCCGCC GCCGAGCTGC CTCCTGCAGC GGCCCCCGCT 
GGTCGTGAGA TGGAGAATAA GGAAACTCTC AAGGGTTTGC ACAAGATGGA TGATCGCCCA 
GAAGAACGAA TGATCAGGGA GAAACTGAAG GCAACCTGTA TGCCAGCCTG GAAGCACGAA 
TGGCTGGAAA GGAGAAATAA GAGAGGCCCT GTGGTGGTAA AACCAATCCC TATTAAAGGA 
GATGGATCTG AAATTAATAA CTTGGCAACT GAGTCTCAAG GAGAGGGCCA GGCGAGTACC 
ACTTCACCAG CTCCCAAAGG CCGACGCAGT CCTTCCCCTG GCAGCTCCCC ATCAGGTCGC 
ACAGTGAAAC CAGACTCTCC AGGAGTAAGG AGAAAAAGAG TTTCCCCCGT GCCATTTCAG 
AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATACAGC 
CCTGAGGAAA CAAACCGCCG TGTAAACAAA GTGATGCGGG CCAGGCTGTA CTTACTGCAG 
CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGATAGTC CAGACAATAA ATACCGGGTG 
TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTATTC 
GTAATGCTCC GAGTATTTCA ACTAGAACCT TCGGACCCAA TGTTATGGAG AAAAACTTTA 
AAGAATTTTG AGGTTGAGAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 
AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 
TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAAGAGGAA 
CAGATGTGTC CTATTTGCTT GTTGGGCATG CTTGATGAAG AAAGTCTTAC AGTGTGTGAA 
GATGGCTGCA GGAACAAGCT GCACCACCAC TGCATGTCTA TTTGGGCAGA AGAATGTAGA 
AGAAATAAAG AACCTTTAAT ATGTCCCCTT TGTAGATCTA AGTGGAGGTC ACATGATTTC 
TACAGTCACG AGCTGTCAAG CCCTGTGGAT TCCCCTTCTT CTCTGCGAGC TGCACAGCAG 
CAAACCATAC AGCAGCCACC TCTGGCTGGA CCACAACGAA GGAATCAGGA GAACAATTTT 
AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA 
TGGATTCAGG TATTTGGAAT GGAACTCGTT GGCTGCTTAT TTTCTAGAAA CTGGAATGTA 
AGAGAGATGG CTCTCAGACG TCTTTCCCAC GATGTTAGTG GGGCTCTGCT ATTGGCAAAT 
GGGGAGAGCA CTGGAAATTC CGGGAGCAAT GGTGGAAACA GCCCGAGTGC CGGAGCTGCC 
AGTGGGTCTT CCCAGACCAG TATCTCAGGA AATGTGGTTG AGGCGTGCTG CAACGTTTTG 
TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACGTTGAGA 
GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT 
CTCCGGCCAG TTGTAGATAC CATCCTAGTC AAGTGCGCAG ATGCCAATAG TCGTACAAGT 
CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGTC AAGCAGGAGA GTTGGCAGTT 
GGCAGAGAAA TACTAAAAGC TGGATCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT 
TGTATTCTTG GAAACCAAAC TGAATCAAAC AACTGGCAAG AACTCCTGGG CCGCCTTTGT 
CTTATAGATA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACA 
GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAGA AGCTGCTGTC CCTCTTAACC 
TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCGATGGTTG GTAAACTCTC CCGAAGGATA 
TATTTGAGTT CTGCAAGAAT GGTTACTGCC GTACCCCACG TATTTTCAAA ACTGTTAGAA 
ATGCTGAATG TGTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT 
GCAGATGAGG TGGAAATCGC TGAGGCCATC CAGCTGGGCA TAGAAGACAC TTTGGATGGT 
CGACATGACA GCTTTTTGCA GGCATCTGTC CTTAACAGCT ATCCAGAAAC CACAGAGAAT 
AGTTCCCTTG GGTGCACAGA CCATTTAGAG AAAACTGGAA AAAGATCGTG TGCTACAAAA 
TTGAGTGCCA GTTCGGAGGA CATTTCTGAC AGACTGGCCA GCATTTCGGT AGGACTTCCT 
AGTTCAACAG CAACGATGGA GCAACCAAAG CCAATGGTTC AGACAAAAGG CAGACCCCAC 
AGTCAGTGTT TGAACTCATC TCTTTTATCT CATCATTCCC AATTAATGTT CCCAGCCTTG 
TCAACCCCTT CTTCATCTGC CCCAGCTGTA CCAGCTGGCA CCACAACAGA TGTCTCTAAG 
CATAGACCTC AGGGATTCAT TCCCTGCAAA ATACCTTCTG CATCTCCTCA AACACAGCGC 
AAGTTTTCTC TACAGTTCCA GAGAAACTGT TCTGAAAACA AAGACTCAGA TAAACTTTCC 
CCAGTCTTTA CTCAGTCAAG ACCCCTGCCC TCCAGTAATA TACACAGGCC AAAGCCATCT 
CGACCTACCC CAGGTAATAC AAGTAAACAG GGAGATGACT CAAAAAATAG CATGACACTT 
GATTTAAACA GTACTTCCAA ATGTGATGAC AGCTTTGGCA GTAGCAGCAA CAGTAGTAAT 
GCTGTGATAC CTAGTGACGA GACAGTGTTC ACCCCAGTGG AGGAGAAATG CAGATTAGAT 
GTCAATACAG AACTCAACTC TAGTATTGAG GACCTTCTTG AAGCGTCTAT GCCCTCAAAT 
GACACAACAG TCACTTTTAA GTCAGAAGTT GCTGTCCTCT CTCCTGAAAA GGATGAAAAT 
GATGATACCT ACAAAGATGA TGTGAATCAT AATCAAAAGT GCAAAGAAAA GATGGAAGCT 
GAAGAAGAAG AAGCGTTAGC AATTGCCATG GCAATGTCAG CATCTCAGGA TGCCCTCCCC 
ATAGTTCCTC AACTACAGGT TGAAAATGGA GAAGATATCA TCATTATTCA ACAGGATACA 
CCAGAGACTC TGCCAGGACA TACCAAAGCA AAACAACCTT ACAGAGAAGA CACTGAATGG 
CTGAAAGGTC AGCAGATAGG TCTTGGAGCA TTTTCTTCTT GTTACCAGGC TCAAGATGTG 
GGAACTGGAA CTTTAATGGC TGTTAAACAG GTGACGTATG TCAGAAATAC ATCATCTGAG 
CAAGAAGAAG TAGTGGAAGC ATTAAGAGAA GAGATAAGAA TGATGAGCCA TCTGAATCAT 
CCAAATATCA TTAGGATGTT GGGAGCCACA TGTGAGAAGA GCAATTATAA TCTCTTCATC 
GAATGGATGG CAGGGGGTTC TGTGGCTCAT TTGCTCAGTA AATATGGAGC CTTCAAGGAA 
TCAGTAGTTA TTAACTATAC TGAACAATTA CTTCGTGGCC TTTCATATCT GCATGAAAAC 
CAGATCATTC ACAGAGATGT CAAAGGTGCC AATTTGCTAA TTGACAGCAC AGGTCAGAGA 
CTGAGAATTG CAGATTTTGG AGCTGCAGCC AGGTTGGCAT CAAAAGGAAC TGGTGCAGGA 
GAGTTTCAGG GACAGTTATT GGGGACAATT GCATTTATGG CACCTGAGGT ACTCAGAGGT 
CAGCAGTATG GTAGGAGCTG TGATGTGTGG AGTGTTGGCT GTGCTATTAT AGAAATGGCT 
TGTGCTAAAC CACCTTGGAA TGCAGAAAAA CATTCCAATC ATCTTGCTTT GATATTCAAG 
ATTGCTAGTG CAACTACTGC TCCATCCATC CCTTCACATT TGTCTCCCGG TTTACGAGAT 
GTGGCTCTTC GTTGTTTAGA ACTTCAACCT CAGGACAGAC CTCCGTCGAG AGAGCTACTG 
AAGCATCCGG TCTTCCGTAC TACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011369350.1
CDS	415..4944
Translation	MAAAAGNRASSSGFPGAGAATPEAGGGGGPLKASSVPGATAGVLREAGSGGRERADWRRRQLRKVRSVELDQLPEPPLFLAASPLSSSTSPSPEPADPAADGSGFQPVAMPQPHGAASRCGAQPPESAAAPDSGAPSPVGADPREKRTPAAELPPAAAPAGREMENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNKRGPVVVKPIPIKGDGSEINNLATESQGEGQASTTSPAPKGRRSPSPGSSPSGRTVKPDSPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNKEPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTIQQPPLAGPQRRNQENNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGSNGGNSPSAGAASGSSQTSISGNVVEACCNVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLNCILGNQTESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEVRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPHVFSKLLEMLNVSSSTHFTRMRRRLMAIADEVEIAEAIQLGIEDTLDGRHDSFLQASVLNSYPETTENSSLGCTDHLEKTGKRSCATKLSASSEDISDRLASISVGLPSSTATMEQPKPMVQTKGRPHSQCLNSSLLSHHSQLMFPALSTPSSSAPAVPAGTTTDVSKHRPQGFIPCKIPSASPQTQRKFSLQFQRNCSENKDSDKLSPVFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDDSKNSMTLDLNSTSKCDDSFGSSSNSSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSNDTTVTFKSEVAVLSPEKDENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_011371048.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011371048.1

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGTGC CGGGGCGGCA 
ACCCCCGAGG CGGGCGGCGG CGGAGGACCG CTTAAGGCGA GCAGCGTGCC TGGGGCGACT 
GCGGGGGTTC TGCGGGAGGC GGGCAGCGGG GGCCGCGAGC GGGCGGACTG GAGGCGGCGG 
CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC 
GCCGCGTCGC CGCTGTCCTC CTCCACTTCC CCGTCGCCGG AGCCTGCAGA CCCGGCGGCG 
GACGGGAGCG GTTTCCAGCC TGTGGCGATG CCGCAGCCTC ACGGAGCCGC GAGCCGCTGC 
GGCGCCCAAC CTCCCGAGTC GGCGGCCGCA CCGGACAGCG GCGCCCCAAG CCCCGTGGGG 
GCCGATCCCA GAGAGAAGCG GACGCCCGCC GCCGAGCTGC CTCCTGCAGC GGCCCCCGCT 
GGTCGTGAGA TGGAGAATAA GGAAACTCTC AAGGGTTTGC ACAAGATGGA TGATCGCCCA 
GAAGAACGAA TGATCAGGGA GAAACTGAAG GCAACCTGTA TGCCAGCCTG GAAGCACGAA 
TGGCTGGAAA GGAGAAATAA GAGAGGCCCT GTGGTGGTAA AACCAATCCC TATTAAAGGA 
GATGGATCTG AAATTAATAA CTTGGCAACT GAGTCTCAAG GAGAGGGCCA GGCGAGTACC 
ACTTCACCAG CTCCCAAAGG CCGACGCAGT CCTTCCCCTG GCAGCTCCCC ATCAGGTCGC 
ACAGTGAAAC CAGACTCTCC AGGAGTAAGG AGAAAAAGAG TTTCCCCCGT GCCATTTCAG 
AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATACAGC 
CCTGAGGAAA CAAACCGCCG TGTAAACAAA GTGATGCGGG CCAGGCTGTA CTTACTGCAG 
CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGATAGTC CAGACAATAA ATACCGGGTG 
TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTATTC 
GTAATGCTCC GAGTATTTCA ACTAGAACCT TCGGACCCAA TGTTATGGAG AAAAACTTTA 
AAGAATTTTG AGGTTGAGAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 
AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 
TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAAGAGGAA 
CAGATGTGTC CTATTTGCTT GTTGGGCATG CTTGATGAAG AAAGTCTTAC AGTGTGTGAA 
GATGGCTGCA GGAACAAGCT GCACCACCAC TGCATGTCTA TTTGGGCAGA AGAATGTAGA 
AGAAATAAAG AACCTTTAAT ATGTCCCCTT TGTAGATCTA AGTGGAGGTC ACATGATTTC 
TACAGTCACG AGCTGTCAAG CCCTGTGGAT TCCCCTTCTT CTCTGCGAGC TGCACAGCAG 
CAAACCATAC AGCAGCCACC TCTGGCTGGA CCACAACGAA GGAATCAGGA GAACAATTTT 
AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA 
TGGATTCAGG TATTTGGAAT GGAACTCGTT GGCTGCTTAT TTTCTAGAAA CTGGAATGTA 
AGAGAGATGG CTCTCAGACG TCTTTCCCAC GATGTTAGTG GGGCTCTGCT ATTGGCAAAT 
GGGGAGAGCA CTGGAAATTC CGGGAGCAAT GGTGGAAACA GCCCGAGTGC CGGAGCTGCC 
AGTGGGTCTT CCCAGACCAG TATCTCAGGA AATGTGGTTG AGGCGTGCTG CAACGTTTTG 
TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACGTTGAGA 
GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT 
CTCCGGCCAG TTGTAGATAC CATCCTAGTC AAGTGCGCAG ATGCCAATAG TCGTACAAGT 
CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGTC AAGCAGGAGA GTTGGCAGTT 
GGCAGAGAAA TACTAAAAGC TGGATCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT 
TGTATTCTTG GAAACCAAAC TGAATCAAAC AACTGGCAAG AACTCCTGGG CCGCCTTTGT 
CTTATAGATA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACA 
GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAGA AGCTGCTGTC CCTCTTAACC 
TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCGATGGTTG GTAAACTCTC CCGAAGGATA 
TATTTGAGTT CTGCAAGAAT GGTTACTGCC GTACCCCACG TATTTTCAAA ACTGTTAGAA 
ATGCTGAATG TGTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT 
GCAGATGAGG TGGAAATCGC TGAGGCCATC CAGCTGGGCA TAGAAGACAC TTTGGATGGT 
CGACATGACA GCTTTTTGCA GGCATCTGTC CTTAACAGCT ATCCAGAAAC CACAGAGAAT 
AGTTCCCTTG GGTGCACAGA CCATTTAGAG AAAACTGGAA AAAGATCGTG TGCTACAAAA 
TTGAGTGCCA GTTCGGAGGA CATTTCTGAC AGACTGGCCA GCATTTCGGT AGGACTTCCT 
AGTTCAACAG CAACGATGGA GCAACCAAAG CCAATGGTTC AGACAAAAGG CAGACCCCAC 
AGTCAGTGTT TGAACTCATC TCTTTTATCT CATCATTCCC AATTAATGTT CCCAGCCTTG 
TCAACCCCTT CTTCATCTGC CCCAGCTGTA CCAGCTGGCA CCACAACAGA TGTCTCTAAG 
CATAGACCTC AGGGATTCAT TCCCTGCAAA ATACCTTCTG CATCTCCTCA AACACAGCGC 
AAGTTTTCTC TACAGTTCCA GAGAAACTGT TCTGAAAACA AAGACTCAGA TAAACTTTCC 
CCAGTCTTTA CTCAGTCAAG ACCCCTGCCC TCCAGTAATA TACACAGGCC AAAGCCATCT 
CGACCTACCC CAGGTAATAC AAGTAAACAG GGAGATGACT CAAAAAATAG CATGACACTT 
GATTTAAACA GTACTTCCAA ATGTGATGAC AGCTTTGGCA GTAGCAGCAA CAGTAGTAAT 
GCTGTGATAC CTAGTGACGA GACAGTGTTC ACCCCAGTGG AGGAGAAATG CAGATTAGAT 
GTCAATACAG AACTCAACTC TAGTATTGAG GACCTTCTTG AAGCGTCTAT GCCCTCAAAT 
GACACAACAG TCACTTTTAA GTCAGAAGTT GCTGTCCTCT CTCCTGAAAA GGATGAAAAT 
GATGATACCT ACAAAGATGA TGTGAATCAT AATCAAAAGT GCAAAGAAAA GATGGAAGCT 
GAAGAAGAAG AAGCGTTAGC AATTGCCATG GCAATGTCAG CATCTCAGGA TGCCCTCCCC 
ATAGTTCCTC AACTACAGGT TGAAAATGGA GAAGATATCA TCATTATTCA ACAGGATACA 
CCAGAGACTC TGCCAGGACA TACCAAAGCA AAACAACCTT ACAGAGAAGA CACTGAATGG 
CTGAAAGGTC AGCAGATAGG TCTTGGAGCA TTTTCTTCTT GTTACCAGGC TCAAGATGTG 
GGAACTGGAA CTTTAATGGC TGTTAAACAG GTGACGTATG TCAGAAATAC ATCATCTGAG 
CAAGAAGAAG TAGTGGAAGC ATTAAGAGAA GAGATAAGAA TGATGAGCCA TCTGAATCAT 
CCAAATATCA TTAGGATGTT GGGAGCCACA TGTGAGAAGA GCAATTATAA TCTCTTCATC 
GAATGGATGG CAGGGGGTTC TGTGGCTCAT TTGCTCAGTA AATATGGAGC CTTCAAGGAA 
TCAGTAGTTA TTAACTATAC TGAACAATTA CTTCGTGGCC TTTCATATCT GCATGAAAAC 
CAGATCATTC ACAGAGATGT CAAAGGTGCC AATTTGCTAA TTGACAGCAC AGGTCAGAGA 
CTGAGAATTG CAGATTTTGG AGCTGCAGCC AGGTTGGCAT CAAAAGGAAC TGGTGCAGGA 
GAGTTTCAGG GACAGTTATT GGGGACAATT GCATTTATGG CACCTGAGGT ACTCAGAGGT 
CAGCAGTATG GTAGGAGCTG TGATGTGTGG AGTGTTGGCT GTGCTATTAT AGAAATGGCT 
TGTGCTAAAC CACCTTGGAA TGCAGAAAAA CATTCCAATC ATCTTGCTTT GATATTCAAG 
ATTGCTAGTG CAACTACTGC TCCATCCATC CCTTCACATT TGTCTCCCGG TTTACGAGAT 
GTGGCTCTTC GTTGTTTAGA ACTTCAACCT CAGGACAGAC CTCCGTCGAG AGAGCTACTG 
AAGCATCCGG TCTTCCGTAC TACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_011371048.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011369350.1 Pteropus vampyrus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X1, mRNA.

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CloneID	OPt54651
Clone ID Related Accession (Same CDS sequence)	XM_011371048.1 , XM_011371048.2
Accession Version	XM_011371048.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888962.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011371048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGTGC CGGGGCGGCA 
ACCCCCGAGG CGGGCGGCGG CGGAGGACCG CTTAAGGCGA GCAGCGTGCC TGGGGCGACT 
GCGGGGGTTC TGCGGGAGGC GGGCAGCGGG GGCCGCGAGC GGGCGGACTG GAGGCGGCGG 
CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC 
GCCGCGTCGC CGCTGTCCTC CTCCACTTCC CCGTCGCCGG AGCCTGCAGA CCCGGCGGCG 
GACGGGAGCG GTTTCCAGCC TGTGGCGATG CCGCAGCCTC ACGGAGCCGC GAGCCGCTGC 
GGCGCCCAAC CTCCCGAGTC GGCGGCCGCA CCGGACAGCG GCGCCCCAAG CCCCGTGGGG 
GCCGATCCCA GAGAGAAGCG GACGCCCGCC GCCGAGCTGC CTCCTGCAGC GGCCCCCGCT 
GGTCGTGAGA TGGAGAATAA GGAAACTCTC AAGGGTTTGC ACAAGATGGA TGATCGCCCA 
GAAGAACGAA TGATCAGGGA GAAACTGAAG GCAACCTGTA TGCCAGCCTG GAAGCACGAA 
TGGCTGGAAA GGAGAAATAA GAGAGGCCCT GTGGTGGTAA AACCAATCCC TATTAAAGGA 
GATGGATCTG AAATTAATAA CTTGGCAACT GAGTCTCAAG GAGAGGGCCA GGCGAGTACC 
ACTTCACCAG CTCCCAAAGG CCGACGCAGT CCTTCCCCTG GCAGCTCCCC ATCAGGTCGC 
ACAGTGAAAC CAGACTCTCC AGGAGTAAGG AGAAAAAGAG TTTCCCCCGT GCCATTTCAG 
AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATACAGC 
CCTGAGGAAA CAAACCGCCG TGTAAACAAA GTGATGCGGG CCAGGCTGTA CTTACTGCAG 
CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGATAGTC CAGACAATAA ATACCGGGTG 
TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTATTC 
GTAATGCTCC GAGTATTTCA ACTAGAACCT TCGGACCCAA TGTTATGGAG AAAAACTTTA 
AAGAATTTTG AGGTTGAGAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 
AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 
TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAAGAGGAA 
CAGATGTGTC CTATTTGCTT GTTGGGCATG CTTGATGAAG AAAGTCTTAC AGTGTGTGAA 
GATGGCTGCA GGAACAAGCT GCACCACCAC TGCATGTCTA TTTGGGCAGA AGAATGTAGA 
AGAAATAAAG AACCTTTAAT ATGTCCCCTT TGTAGATCTA AGTGGAGGTC ACATGATTTC 
TACAGTCACG AGCTGTCAAG CCCTGTGGAT TCCCCTTCTT CTCTGCGAGC TGCACAGCAG 
CAAACCATAC AGCAGCCACC TCTGGCTGGA CCACAACGAA GGAATCAGGA GAACAATTTT 
AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA 
TGGATTCAGG TATTTGGAAT GGAACTCGTT GGCTGCTTAT TTTCTAGAAA CTGGAATGTA 
AGAGAGATGG CTCTCAGACG TCTTTCCCAC GATGTTAGTG GGGCTCTGCT ATTGGCAAAT 
GGGGAGAGCA CTGGAAATTC CGGGAGCAAT GGTGGAAACA GCCCGAGTGC CGGAGCTGCC 
AGTGGGTCTT CCCAGACCAG TATCTCAGGA AATGTGGTTG AGGCGTGCTG CAACGTTTTG 
TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACGTTGAGA 
GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT 
CTCCGGCCAG TTGTAGATAC CATCCTAGTC AAGTGCGCAG ATGCCAATAG TCGTACAAGT 
CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGTC AAGCAGGAGA GTTGGCAGTT 
GGCAGAGAAA TACTAAAAGC TGGATCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT 
TGTATTCTTG GAAACCAAAC TGAATCAAAC AACTGGCAAG AACTCCTGGG CCGCCTTTGT 
CTTATAGATA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACA 
GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAGA AGCTGCTGTC CCTCTTAACC 
TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCGATGGTTG GTAAACTCTC CCGAAGGATA 
TATTTGAGTT CTGCAAGAAT GGTTACTGCC GTACCCCACG TATTTTCAAA ACTGTTAGAA 
ATGCTGAATG TGTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT 
GCAGATGAGG TGGAAATCGC TGAGGCCATC CAGCTGGGCA TAGAAGACAC TTTGGATGGT 
CGACATGACA GCTTTTTGCA GGCATCTGTC CTTAACAGCT ATCCAGAAAC CACAGAGAAT 
AGTTCCCTTG GGTGCACAGA CCATTTAGAG AAAACTGGAA AAAGATCGTG TGCTACAAAA 
TTGAGTGCCA GTTCGGAGGA CATTTCTGAC AGACTGGCCA GCATTTCGGT AGGACTTCCT 
AGTTCAACAG CAACGATGGA GCAACCAAAG CCAATGGTTC AGACAAAAGG CAGACCCCAC 
AGTCAGTGTT TGAACTCATC TCTTTTATCT CATCATTCCC AATTAATGTT CCCAGCCTTG 
TCAACCCCTT CTTCATCTGC CCCAGCTGTA CCAGCTGGCA CCACAACAGA TGTCTCTAAG 
CATAGACCTC AGGGATTCAT TCCCTGCAAA ATACCTTCTG CATCTCCTCA AACACAGCGC 
AAGTTTTCTC TACAGTTCCA GAGAAACTGT TCTGAAAACA AAGACTCAGA TAAACTTTCC 
CCAGTCTTTA CTCAGTCAAG ACCCCTGCCC TCCAGTAATA TACACAGGCC AAAGCCATCT 
CGACCTACCC CAGGTAATAC AAGTAAACAG GGAGATGACT CAAAAAATAG CATGACACTT 
GATTTAAACA GTACTTCCAA ATGTGATGAC AGCTTTGGCA GTAGCAGCAA CAGTAGTAAT 
GCTGTGATAC CTAGTGACGA GACAGTGTTC ACCCCAGTGG AGGAGAAATG CAGATTAGAT 
GTCAATACAG AACTCAACTC TAGTATTGAG GACCTTCTTG AAGCGTCTAT GCCCTCAAAT 
GACACAACAG TCACTTTTAA GTCAGAAGTT GCTGTCCTCT CTCCTGAAAA GGATGAAAAT 
GATGATACCT ACAAAGATGA TGTGAATCAT AATCAAAAGT GCAAAGAAAA GATGGAAGCT 
GAAGAAGAAG AAGCGTTAGC AATTGCCATG GCAATGTCAG CATCTCAGGA TGCCCTCCCC 
ATAGTTCCTC AACTACAGGT TGAAAATGGA GAAGATATCA TCATTATTCA ACAGGATACA 
CCAGAGACTC TGCCAGGACA TACCAAAGCA AAACAACCTT ACAGAGAAGA CACTGAATGG 
CTGAAAGGTC AGCAGATAGG TCTTGGAGCA TTTTCTTCTT GTTACCAGGC TCAAGATGTG 
GGAACTGGAA CTTTAATGGC TGTTAAACAG GTGACGTATG TCAGAAATAC ATCATCTGAG 
CAAGAAGAAG TAGTGGAAGC ATTAAGAGAA GAGATAAGAA TGATGAGCCA TCTGAATCAT 
CCAAATATCA TTAGGATGTT GGGAGCCACA TGTGAGAAGA GCAATTATAA TCTCTTCATC 
GAATGGATGG CAGGGGGTTC TGTGGCTCAT TTGCTCAGTA AATATGGAGC CTTCAAGGAA 
TCAGTAGTTA TTAACTATAC TGAACAATTA CTTCGTGGCC TTTCATATCT GCATGAAAAC 
CAGATCATTC ACAGAGATGT CAAAGGTGCC AATTTGCTAA TTGACAGCAC AGGTCAGAGA 
CTGAGAATTG CAGATTTTGG AGCTGCAGCC AGGTTGGCAT CAAAAGGAAC TGGTGCAGGA 
GAGTTTCAGG GACAGTTATT GGGGACAATT GCATTTATGG CACCTGAGGT ACTCAGAGGT 
CAGCAGTATG GTAGGAGCTG TGATGTGTGG AGTGTTGGCT GTGCTATTAT AGAAATGGCT 
TGTGCTAAAC CACCTTGGAA TGCAGAAAAA CATTCCAATC ATCTTGCTTT GATATTCAAG 
ATTGCTAGTG CAACTACTGC TCCATCCATC CCTTCACATT TGTCTCCCGG TTTACGAGAT 
GTGGCTCTTC GTTGTTTAGA ACTTCAACCT CAGGACAGAC CTCCGTCGAG AGAGCTACTG 
AAGCATCCGG TCTTCCGTAC TACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011369350.1
CDS	1067..5596
Translation	MAAAAGNRASSSGFPGAGAATPEAGGGGGPLKASSVPGATAGVLREAGSGGRERADWRRRQLRKVRSVELDQLPEPPLFLAASPLSSSTSPSPEPADPAADGSGFQPVAMPQPHGAASRCGAQPPESAAAPDSGAPSPVGADPREKRTPAAELPPAAAPAGREMENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNKRGPVVVKPIPIKGDGSEINNLATESQGEGQASTTSPAPKGRRSPSPGSSPSGRTVKPDSPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNKEPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTIQQPPLAGPQRRNQENNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGSNGGNSPSAGAASGSSQTSISGNVVEACCNVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLNCILGNQTESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEVRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPHVFSKLLEMLNVSSSTHFTRMRRRLMAIADEVEIAEAIQLGIEDTLDGRHDSFLQASVLNSYPETTENSSLGCTDHLEKTGKRSCATKLSASSEDISDRLASISVGLPSSTATMEQPKPMVQTKGRPHSQCLNSSLLSHHSQLMFPALSTPSSSAPAVPAGTTTDVSKHRPQGFIPCKIPSASPQTQRKFSLQFQRNCSENKDSDKLSPVFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDDSKNSMTLDLNSTSKCDDSFGSSSNSSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSNDTTVTFKSEVAVLSPEKDENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_011371048.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011371048.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGTGC CGGGGCGGCA 
ACCCCCGAGG CGGGCGGCGG CGGAGGACCG CTTAAGGCGA GCAGCGTGCC TGGGGCGACT 
GCGGGGGTTC TGCGGGAGGC GGGCAGCGGG GGCCGCGAGC GGGCGGACTG GAGGCGGCGG 
CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC 
GCCGCGTCGC CGCTGTCCTC CTCCACTTCC CCGTCGCCGG AGCCTGCAGA CCCGGCGGCG 
GACGGGAGCG GTTTCCAGCC TGTGGCGATG CCGCAGCCTC ACGGAGCCGC GAGCCGCTGC 
GGCGCCCAAC CTCCCGAGTC GGCGGCCGCA CCGGACAGCG GCGCCCCAAG CCCCGTGGGG 
GCCGATCCCA GAGAGAAGCG GACGCCCGCC GCCGAGCTGC CTCCTGCAGC GGCCCCCGCT 
GGTCGTGAGA TGGAGAATAA GGAAACTCTC AAGGGTTTGC ACAAGATGGA TGATCGCCCA 
GAAGAACGAA TGATCAGGGA GAAACTGAAG GCAACCTGTA TGCCAGCCTG GAAGCACGAA 
TGGCTGGAAA GGAGAAATAA GAGAGGCCCT GTGGTGGTAA AACCAATCCC TATTAAAGGA 
GATGGATCTG AAATTAATAA CTTGGCAACT GAGTCTCAAG GAGAGGGCCA GGCGAGTACC 
ACTTCACCAG CTCCCAAAGG CCGACGCAGT CCTTCCCCTG GCAGCTCCCC ATCAGGTCGC 
ACAGTGAAAC CAGACTCTCC AGGAGTAAGG AGAAAAAGAG TTTCCCCCGT GCCATTTCAG 
AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATACAGC 
CCTGAGGAAA CAAACCGCCG TGTAAACAAA GTGATGCGGG CCAGGCTGTA CTTACTGCAG 
CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGATAGTC CAGACAATAA ATACCGGGTG 
TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTATTC 
GTAATGCTCC GAGTATTTCA ACTAGAACCT TCGGACCCAA TGTTATGGAG AAAAACTTTA 
AAGAATTTTG AGGTTGAGAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 
AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 
TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAAGAGGAA 
CAGATGTGTC CTATTTGCTT GTTGGGCATG CTTGATGAAG AAAGTCTTAC AGTGTGTGAA 
GATGGCTGCA GGAACAAGCT GCACCACCAC TGCATGTCTA TTTGGGCAGA AGAATGTAGA 
AGAAATAAAG AACCTTTAAT ATGTCCCCTT TGTAGATCTA AGTGGAGGTC ACATGATTTC 
TACAGTCACG AGCTGTCAAG CCCTGTGGAT TCCCCTTCTT CTCTGCGAGC TGCACAGCAG 
CAAACCATAC AGCAGCCACC TCTGGCTGGA CCACAACGAA GGAATCAGGA GAACAATTTT 
AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA 
TGGATTCAGG TATTTGGAAT GGAACTCGTT GGCTGCTTAT TTTCTAGAAA CTGGAATGTA 
AGAGAGATGG CTCTCAGACG TCTTTCCCAC GATGTTAGTG GGGCTCTGCT ATTGGCAAAT 
GGGGAGAGCA CTGGAAATTC CGGGAGCAAT GGTGGAAACA GCCCGAGTGC CGGAGCTGCC 
AGTGGGTCTT CCCAGACCAG TATCTCAGGA AATGTGGTTG AGGCGTGCTG CAACGTTTTG 
TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACGTTGAGA 
GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT 
CTCCGGCCAG TTGTAGATAC CATCCTAGTC AAGTGCGCAG ATGCCAATAG TCGTACAAGT 
CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGTC AAGCAGGAGA GTTGGCAGTT 
GGCAGAGAAA TACTAAAAGC TGGATCCATT GGTATTGGTG GTGTTGATTA TGTCTTAAAT 
TGTATTCTTG GAAACCAAAC TGAATCAAAC AACTGGCAAG AACTCCTGGG CCGCCTTTGT 
CTTATAGATA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACA 
GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAGA AGCTGCTGTC CCTCTTAACC 
TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCGATGGTTG GTAAACTCTC CCGAAGGATA 
TATTTGAGTT CTGCAAGAAT GGTTACTGCC GTACCCCACG TATTTTCAAA ACTGTTAGAA 
ATGCTGAATG TGTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT 
GCAGATGAGG TGGAAATCGC TGAGGCCATC CAGCTGGGCA TAGAAGACAC TTTGGATGGT 
CGACATGACA GCTTTTTGCA GGCATCTGTC CTTAACAGCT ATCCAGAAAC CACAGAGAAT 
AGTTCCCTTG GGTGCACAGA CCATTTAGAG AAAACTGGAA AAAGATCGTG TGCTACAAAA 
TTGAGTGCCA GTTCGGAGGA CATTTCTGAC AGACTGGCCA GCATTTCGGT AGGACTTCCT 
AGTTCAACAG CAACGATGGA GCAACCAAAG CCAATGGTTC AGACAAAAGG CAGACCCCAC 
AGTCAGTGTT TGAACTCATC TCTTTTATCT CATCATTCCC AATTAATGTT CCCAGCCTTG 
TCAACCCCTT CTTCATCTGC CCCAGCTGTA CCAGCTGGCA CCACAACAGA TGTCTCTAAG 
CATAGACCTC AGGGATTCAT TCCCTGCAAA ATACCTTCTG CATCTCCTCA AACACAGCGC 
AAGTTTTCTC TACAGTTCCA GAGAAACTGT TCTGAAAACA AAGACTCAGA TAAACTTTCC 
CCAGTCTTTA CTCAGTCAAG ACCCCTGCCC TCCAGTAATA TACACAGGCC AAAGCCATCT 
CGACCTACCC CAGGTAATAC AAGTAAACAG GGAGATGACT CAAAAAATAG CATGACACTT 
GATTTAAACA GTACTTCCAA ATGTGATGAC AGCTTTGGCA GTAGCAGCAA CAGTAGTAAT 
GCTGTGATAC CTAGTGACGA GACAGTGTTC ACCCCAGTGG AGGAGAAATG CAGATTAGAT 
GTCAATACAG AACTCAACTC TAGTATTGAG GACCTTCTTG AAGCGTCTAT GCCCTCAAAT 
GACACAACAG TCACTTTTAA GTCAGAAGTT GCTGTCCTCT CTCCTGAAAA GGATGAAAAT 
GATGATACCT ACAAAGATGA TGTGAATCAT AATCAAAAGT GCAAAGAAAA GATGGAAGCT 
GAAGAAGAAG AAGCGTTAGC AATTGCCATG GCAATGTCAG CATCTCAGGA TGCCCTCCCC 
ATAGTTCCTC AACTACAGGT TGAAAATGGA GAAGATATCA TCATTATTCA ACAGGATACA 
CCAGAGACTC TGCCAGGACA TACCAAAGCA AAACAACCTT ACAGAGAAGA CACTGAATGG 
CTGAAAGGTC AGCAGATAGG TCTTGGAGCA TTTTCTTCTT GTTACCAGGC TCAAGATGTG 
GGAACTGGAA CTTTAATGGC TGTTAAACAG GTGACGTATG TCAGAAATAC ATCATCTGAG 
CAAGAAGAAG TAGTGGAAGC ATTAAGAGAA GAGATAAGAA TGATGAGCCA TCTGAATCAT 
CCAAATATCA TTAGGATGTT GGGAGCCACA TGTGAGAAGA GCAATTATAA TCTCTTCATC 
GAATGGATGG CAGGGGGTTC TGTGGCTCAT TTGCTCAGTA AATATGGAGC CTTCAAGGAA 
TCAGTAGTTA TTAACTATAC TGAACAATTA CTTCGTGGCC TTTCATATCT GCATGAAAAC 
CAGATCATTC ACAGAGATGT CAAAGGTGCC AATTTGCTAA TTGACAGCAC AGGTCAGAGA 
CTGAGAATTG CAGATTTTGG AGCTGCAGCC AGGTTGGCAT CAAAAGGAAC TGGTGCAGGA 
GAGTTTCAGG GACAGTTATT GGGGACAATT GCATTTATGG CACCTGAGGT ACTCAGAGGT 
CAGCAGTATG GTAGGAGCTG TGATGTGTGG AGTGTTGGCT GTGCTATTAT AGAAATGGCT 
TGTGCTAAAC CACCTTGGAA TGCAGAAAAA CATTCCAATC ATCTTGCTTT GATATTCAAG 
ATTGCTAGTG CAACTACTGC TCCATCCATC CCTTCACATT TGTCTCCCGG TTTACGAGAT 
GTGGCTCTTC GTTGTTTAGA ACTTCAACCT CAGGACAGAC CTCCGTCGAG AGAGCTACTG 
AAGCATCCGG TCTTCCGTAC TACATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MAP3K1 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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