WNK1 gene cDNA ORF clone, Pteropus vampyrus(large flying fox)

mRNA	Protein	Name
XM_023535116.1	XP_023390884.1	serine/threonine-protein kinase WNK1 isoform X6
XM_011367824.2	XP_011366126.1	serine/threonine-protein kinase WNK1 isoform X2
XM_011367825.2	XP_011366127.1	serine/threonine-protein kinase WNK1 isoform X3
XM_011367826.1	XP_011366128.1	serine/threonine-protein kinase WNK1 isoform X4
XM_011367828.1	XP_011366130.1	serine/threonine-protein kinase WNK1 isoform X6
XM_011367827.1	XP_011366129.1	serine/threonine-protein kinase WNK1 isoform X5
XM_011367827.2	XP_011366129.1	serine/threonine-protein kinase WNK1 isoform X9
XM_011367830.1	XP_011366132.1	serine/threonine-protein kinase WNK1 isoform X7
XM_011367825.1	XP_011366127.1	serine/threonine-protein kinase WNK1 isoform X3
XM_011367828.2	XP_011366130.1	serine/threonine-protein kinase WNK1 isoform X10
XM_011367830.2	XP_011366132.1	serine/threonine-protein kinase WNK1 isoform X11
XM_023535118.1	XP_023390886.1	serine/threonine-protein kinase WNK1 isoform X8
XM_023535117.1	XP_023390885.1	serine/threonine-protein kinase WNK1 isoform X7
XM_023535115.1	XP_023390883.1	serine/threonine-protein kinase WNK1 isoform X5
XM_011367822.2	XP_011366124.1	serine/threonine-protein kinase WNK1 isoform X1
XM_011367822.1	XP_011366124.1	serine/threonine-protein kinase WNK1 isoform X1
XM_011367824.1	XP_011366126.1	serine/threonine-protein kinase WNK1 isoform X2
XM_023535114.1	XP_023390882.1	serine/threonine-protein kinase WNK1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt52294	XM_011367822.2 Latest version!	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OPt52295	XM_011367824.2 Latest version!	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OPt52296	XM_011367825.2 Latest version!	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OPt52297	XM_011367826.1 Latest version!	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt52298	XM_011367827.2 Latest version!	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt52299	XM_011367828.2 Latest version!	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt72364	XM_023535114.1 Latest version!	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OPt72365	XM_023535115.1 Latest version!	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OPt72366	XM_023535116.1 Latest version!	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt72367	XM_023535117.1 Latest version!	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt72368	XM_023535118.1 Latest version!	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt52300	XM_011367830.2 Latest version!	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OPt52298	XM_011367827.1	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt52296	XM_011367825.1	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OPt52299	XM_011367828.1	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt52300	XM_011367830.1	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OPt52294	XM_011367822.1	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OPt52295	XM_011367824.1	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

WNK1 ( XM_011367822.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011366124.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPt52294
Clone ID Related Accession (Same CDS sequence)	XM_011367822.2 , XM_011367822.1
Accession Version	XM_011367822.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011367822.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG TCTCTCAAAT TCCTGTATCA ACTCCTCATG TGTCCGCGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCCTTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCCGC AATCCCAGGG GGATCAACTA TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT CCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTCCCTCTTC CAGCGTGGCT TCTGTTTGCA TTCCTTCTAC AGTCTTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTTT GGTTCCTGTG GGCAGTGTAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCACTTT GGTCTCTTCT ATAGATAGTG CACATTCAGA TGTTGCTTCA 
GGTATGAGCG ATGGCAATGA GAATGTGCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGTCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAGTTA 
GAGACTCACA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCCGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCGTTT 
GTGGATCAAG TACGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCCG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA AGGAGAGTTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GCATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC TGGAAGACGG 
TTTATAGTGA GTCCTGTACC AGAAAGCCGA TTACGAGAAT CAAAAATTTT CACCAGTGAA 
ATATCAGATA CGGTTGCCGC CTCTACATCT CATGGCCCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTTA GACATGCCCA AGTTGCAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATATAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAATCCCA ACGACAGCAG CAGCCGTACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC ATTTCCACAT CAATAATTCA ATCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCACC TGCGTCTGAA TCACCAATTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CAACAGTTGT CTCAGTATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TATAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCAGTGC TGCTGCCCCA GGGGCTAAGT CTCCACCTTT GTTATCTGAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCACTCCA 
TTCCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCACTAGATA AAACATCTCA TAGCAGTACA 
ACTGGACTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAACC TGGTGGATTA CATCCTTTGG TCATCCCATC AGCTGTAGTT 
TCTACTCCTG TCCTTTCCCA AGCATCAGGA CCTATTTCTA CCCCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CTCAACCAGC CCCATACGCA CTGCCCTGAA 
ATAGATGCTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TCTAGAGGAA 
AAGTTGCGGT CTCTCTTTAG TGAACACAGC TCATCTGGCG CTCAACATGC CTCTGTTGCG 
CTGGAGACAT CACTAGTAGT AGAGACCAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CATTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCA 
GTCAGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGGCT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG AAGGTTCTGT CCCAGCAGCT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAGAAAG AGGGTAAAAC TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGAC TCTAATGGGG TAACCATCCA TGGCATCTCT 
TCAGATATGC CAGATAGTGC CCACAAAACT CCTGCCGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGGCG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGCTCACT GAGGCAAAGA AAGAGGGACC GACAGCATCT 
CCTCCTTTTA TGGATTTGGA ACAACCTGTT CTTCCTGCTG CAATACCAAA GAAAGAAAAG 
CCTGAACTGT CAGAGCCTTC ACATCTGAAT GGGCCATCTT CTGATTTGGA GGCTGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CCCCCCACCA ACTGAGCTCA 
AAGAGCCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAT 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAAATTCAA GACCTGCAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTAC ACCCCCAGCA GACCCTCCAC CCTCCAGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG GACCAGCAGC 
ACAAACACTG TCGGGGGAAC AGTGAATAGC CAAGCTGCCC AAGCTCAGCC TCCTGCCATG 
ACGTCCAGCA GGAAGGGCAC GTTCACAGAT GACCTGCACA AGTTGGTAGA CAATTGGGCC 
AGAGATGCCA TGAATCTTTC AGGCAGGAGA GGAAGCAAAG GACACATGAA TTATGAGGGC 
CCTGGAATGG CAAGGAAGTT CTCTGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAT 
CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG 
TCTATGTGCC CCCCACAGCA GTATGGTTTT CCACCTACCC CCTTTGGCAC TCAGTGGAGT 
GGGACAGGTG GCCCAGCACC ACAGCCACTT GGCCAGTTCC AACCTGTGGG AACCACCTCC 
TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC 
CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011366124.1
CDS	667..7821
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHPQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPVSQIPVSTPHVSAAQTGFSTLPITIAAGLNQPLLTLASSATAAAIPGGSTMAPSQLPALLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIAPSSSVASVCIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLLVPVGSVGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPEAIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011367822.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011367822.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG TCTCTCAAAT TCCTGTATCA ACTCCTCATG TGTCCGCGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCCTTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCCGC AATCCCAGGG GGATCAACTA TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT CCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTCCCTCTTC CAGCGTGGCT TCTGTTTGCA TTCCTTCTAC AGTCTTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTTT GGTTCCTGTG GGCAGTGTAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCACTTT GGTCTCTTCT ATAGATAGTG CACATTCAGA TGTTGCTTCA 
GGTATGAGCG ATGGCAATGA GAATGTGCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGTCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAGTTA 
GAGACTCACA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCCGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCGTTT 
GTGGATCAAG TACGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCCG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA AGGAGAGTTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GCATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC TGGAAGACGG 
TTTATAGTGA GTCCTGTACC AGAAAGCCGA TTACGAGAAT CAAAAATTTT CACCAGTGAA 
ATATCAGATA CGGTTGCCGC CTCTACATCT CATGGCCCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTTA GACATGCCCA AGTTGCAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATATAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAATCCCA ACGACAGCAG CAGCCGTACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC ATTTCCACAT CAATAATTCA ATCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCACC TGCGTCTGAA TCACCAATTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CAACAGTTGT CTCAGTATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TATAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCAGTGC TGCTGCCCCA GGGGCTAAGT CTCCACCTTT GTTATCTGAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCACTCCA 
TTCCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCACTAGATA AAACATCTCA TAGCAGTACA 
ACTGGACTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAACC TGGTGGATTA CATCCTTTGG TCATCCCATC AGCTGTAGTT 
TCTACTCCTG TCCTTTCCCA AGCATCAGGA CCTATTTCTA CCCCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CTCAACCAGC CCCATACGCA CTGCCCTGAA 
ATAGATGCTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TCTAGAGGAA 
AAGTTGCGGT CTCTCTTTAG TGAACACAGC TCATCTGGCG CTCAACATGC CTCTGTTGCG 
CTGGAGACAT CACTAGTAGT AGAGACCAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CATTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCA 
GTCAGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGGCT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG AAGGTTCTGT CCCAGCAGCT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAGAAAG AGGGTAAAAC TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGAC TCTAATGGGG TAACCATCCA TGGCATCTCT 
TCAGATATGC CAGATAGTGC CCACAAAACT CCTGCCGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGGCG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGCTCACT GAGGCAAAGA AAGAGGGACC GACAGCATCT 
CCTCCTTTTA TGGATTTGGA ACAACCTGTT CTTCCTGCTG CAATACCAAA GAAAGAAAAG 
CCTGAACTGT CAGAGCCTTC ACATCTGAAT GGGCCATCTT CTGATTTGGA GGCTGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CCCCCCACCA ACTGAGCTCA 
AAGAGCCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAT 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAAATTCAA GACCTGCAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTAC ACCCCCAGCA GACCCTCCAC CCTCCAGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG GACCAGCAGC 
ACAAACACTG TCGGGGGAAC AGTGAATAGC CAAGCTGCCC AAGCTCAGCC TCCTGCCATG 
ACGTCCAGCA GGAAGGGCAC GTTCACAGAT GACCTGCACA AGTTGGTAGA CAATTGGGCC 
AGAGATGCCA TGAATCTTTC AGGCAGGAGA GGAAGCAAAG GACACATGAA TTATGAGGGC 
CCTGGAATGG CAAGGAAGTT CTCTGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAT 
CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG 
TCTATGTGCC CCCCACAGCA GTATGGTTTT CCACCTACCC CCTTTGGCAC TCAGTGGAGT 
GGGACAGGTG GCCCAGCACC ACAGCCACTT GGCCAGTTCC AACCTGTGGG AACCACCTCC 
TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC 
CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011367822.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011366124.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

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CloneID	OPt52294
Clone ID Related Accession (Same CDS sequence)	XM_011367822.2 , XM_011367822.1
Accession Version	XM_011367822.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG TCTCTCAAAT TCCTGTATCA ACTCCTCATG TGTCCGCGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCCTTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCCGC AATCCCAGGG GGATCAACTA TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT CCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTCCCTCTTC CAGCGTGGCT TCTGTTTGCA TTCCTTCTAC AGTCTTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTTT GGTTCCTGTG GGCAGTGTAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCACTTT GGTCTCTTCT ATAGATAGTG CACATTCAGA TGTTGCTTCA 
GGTATGAGCG ATGGCAATGA GAATGTGCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGTCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAGTTA 
GAGACTCACA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCCGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCGTTT 
GTGGATCAAG TACGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCCG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA AGGAGAGTTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GCATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC TGGAAGACGG 
TTTATAGTGA GTCCTGTACC AGAAAGCCGA TTACGAGAAT CAAAAATTTT CACCAGTGAA 
ATATCAGATA CGGTTGCCGC CTCTACATCT CATGGCCCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTTA GACATGCCCA AGTTGCAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATATAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAATCCCA ACGACAGCAG CAGCCGTACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC ATTTCCACAT CAATAATTCA ATCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCACC TGCGTCTGAA TCACCAATTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CAACAGTTGT CTCAGTATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TATAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCAGTGC TGCTGCCCCA GGGGCTAAGT CTCCACCTTT GTTATCTGAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCACTCCA 
TTCCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCACTAGATA AAACATCTCA TAGCAGTACA 
ACTGGACTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAACC TGGTGGATTA CATCCTTTGG TCATCCCATC AGCTGTAGTT 
TCTACTCCTG TCCTTTCCCA AGCATCAGGA CCTATTTCTA CCCCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CTCAACCAGC CCCATACGCA CTGCCCTGAA 
ATAGATGCTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TCTAGAGGAA 
AAGTTGCGGT CTCTCTTTAG TGAACACAGC TCATCTGGCG CTCAACATGC CTCTGTTGCG 
CTGGAGACAT CACTAGTAGT AGAGACCAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CATTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCA 
GTCAGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGGCT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG AAGGTTCTGT CCCAGCAGCT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAGAAAG AGGGTAAAAC TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGAC TCTAATGGGG TAACCATCCA TGGCATCTCT 
TCAGATATGC CAGATAGTGC CCACAAAACT CCTGCCGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGGCG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGCTCACT GAGGCAAAGA AAGAGGGACC GACAGCATCT 
CCTCCTTTTA TGGATTTGGA ACAACCTGTT CTTCCTGCTG CAATACCAAA GAAAGAAAAG 
CCTGAACTGT CAGAGCCTTC ACATCTGAAT GGGCCATCTT CTGATTTGGA GGCTGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CCCCCCACCA ACTGAGCTCA 
AAGAGCCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAT 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAAATTCAA GACCTGCAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTAC ACCCCCAGCA GACCCTCCAC CCTCCAGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG GACCAGCAGC 
ACAAACACTG TCGGGGGAAC AGTGAATAGC CAAGCTGCCC AAGCTCAGCC TCCTGCCATG 
ACGTCCAGCA GGAAGGGCAC GTTCACAGAT GACCTGCACA AGTTGGTAGA CAATTGGGCC 
AGAGATGCCA TGAATCTTTC AGGCAGGAGA GGAAGCAAAG GACACATGAA TTATGAGGGC 
CCTGGAATGG CAAGGAAGTT CTCTGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAT 
CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG 
TCTATGTGCC CCCCACAGCA GTATGGTTTT CCACCTACCC CCTTTGGCAC TCAGTGGAGT 
GGGACAGGTG GCCCAGCACC ACAGCCACTT GGCCAGTTCC AACCTGTGGG AACCACCTCC 
TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC 
CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011366124.1
CDS	1012..8166
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHPQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPVSQIPVSTPHVSAAQTGFSTLPITIAAGLNQPLLTLASSATAAAIPGGSTMAPSQLPALLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIAPSSSVASVCIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLLVPVGSVGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPEAIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011367822.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011367822.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG TCTCTCAAAT TCCTGTATCA ACTCCTCATG TGTCCGCGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCCTTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCCGC AATCCCAGGG GGATCAACTA TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT CCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTCCCTCTTC CAGCGTGGCT TCTGTTTGCA TTCCTTCTAC AGTCTTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTTT GGTTCCTGTG GGCAGTGTAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCACTTT GGTCTCTTCT ATAGATAGTG CACATTCAGA TGTTGCTTCA 
GGTATGAGCG ATGGCAATGA GAATGTGCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGTCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAGTTA 
GAGACTCACA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCCGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCGTTT 
GTGGATCAAG TACGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCCG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA AGGAGAGTTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GCATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC TGGAAGACGG 
TTTATAGTGA GTCCTGTACC AGAAAGCCGA TTACGAGAAT CAAAAATTTT CACCAGTGAA 
ATATCAGATA CGGTTGCCGC CTCTACATCT CATGGCCCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTTA GACATGCCCA AGTTGCAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATATAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAATCCCA ACGACAGCAG CAGCCGTACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC ATTTCCACAT CAATAATTCA ATCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCACC TGCGTCTGAA TCACCAATTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CAACAGTTGT CTCAGTATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TATAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCAGTGC TGCTGCCCCA GGGGCTAAGT CTCCACCTTT GTTATCTGAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCACTCCA 
TTCCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCACTAGATA AAACATCTCA TAGCAGTACA 
ACTGGACTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAACC TGGTGGATTA CATCCTTTGG TCATCCCATC AGCTGTAGTT 
TCTACTCCTG TCCTTTCCCA AGCATCAGGA CCTATTTCTA CCCCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CTCAACCAGC CCCATACGCA CTGCCCTGAA 
ATAGATGCTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TCTAGAGGAA 
AAGTTGCGGT CTCTCTTTAG TGAACACAGC TCATCTGGCG CTCAACATGC CTCTGTTGCG 
CTGGAGACAT CACTAGTAGT AGAGACCAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CATTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCA 
GTCAGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGGCT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG AAGGTTCTGT CCCAGCAGCT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAGAAAG AGGGTAAAAC TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGAC TCTAATGGGG TAACCATCCA TGGCATCTCT 
TCAGATATGC CAGATAGTGC CCACAAAACT CCTGCCGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGGCG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGCTCACT GAGGCAAAGA AAGAGGGACC GACAGCATCT 
CCTCCTTTTA TGGATTTGGA ACAACCTGTT CTTCCTGCTG CAATACCAAA GAAAGAAAAG 
CCTGAACTGT CAGAGCCTTC ACATCTGAAT GGGCCATCTT CTGATTTGGA GGCTGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CCCCCCACCA ACTGAGCTCA 
AAGAGCCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAT 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAAATTCAA GACCTGCAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTAC ACCCCCAGCA GACCCTCCAC CCTCCAGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG GACCAGCAGC 
ACAAACACTG TCGGGGGAAC AGTGAATAGC CAAGCTGCCC AAGCTCAGCC TCCTGCCATG 
ACGTCCAGCA GGAAGGGCAC GTTCACAGAT GACCTGCACA AGTTGGTAGA CAATTGGGCC 
AGAGATGCCA TGAATCTTTC AGGCAGGAGA GGAAGCAAAG GACACATGAA TTATGAGGGC 
CCTGGAATGG CAAGGAAGTT CTCTGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAT 
CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG 
TCTATGTGCC CCCCACAGCA GTATGGTTTT CCACCTACCC CCTTTGGCAC TCAGTGGAGT 
GGGACAGGTG GCCCAGCACC ACAGCCACTT GGCCAGTTCC AACCTGTGGG AACCACCTCC 
TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC 
CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011367824.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011366126.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

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CloneID	OPt52295
Clone ID Related Accession (Same CDS sequence)	XM_011367824.2 , XM_011367824.1
Accession Version	XM_011367824.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011367824.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCATGGAAT ACAGCAGACA GCCCCTCCTC AACAGACAGT ACAGTATTCA 
CTTCCACAGA CATCAACCTC CAGTGAGGCC ACTACTGCAC AGCCAGTGAG TCAGCCTCAG 
GCTCCACAGG TCTTGCCTCA AGCATCAGCT GGAAAACAGC TTCCAGTTTC CCAGCCAGTA 
CCAACTGTCC AAGGCGAACC TCAGATCTCA GTTGCGACAC AACCCTCAGT TGTTCCAGTG 
CATTCTGGTG CTCATTTCCT CCCTATGGGA CAGCCACTTC CTACTTCCTT ACTCCCTCAG 
TACCCTGTCT CTCAAATTCC TGTATCAACT CCTCATGTGT CCGCGGCTCA GACAGGTTTC 
TCAACCCTTC CCATCACAAT TGCAGCTGGC CTTAATCAGC CTCTGCTCAC CTTGGCTTCA 
TCTGCTACAG CAGCCGCAAT CCCAGGGGGA TCAACTATGG CTCCTAGTCA GCTACCAGCC 
CTTCTGCAGC CTGTGACTCA GCTGCCAAGT CAGGTTCACC CACAGCTCCT GCAGCCAGCA 
GTTCAGTCCA TGGGAATACC AGCTAACCTT GGACAAGCTG CTGAGGTTCC ACTTCCCTCT 
GGAGATGTTC TATACCAGGG CTTCCCACCT CGACTGCCAC CACAATACCC AGGAGATTCA 
AATATTGCTC CCTCTTCCAG CGTGGCTTCT GTTTGCATTC CTTCTACAGT CTTATCCCCT 
CCAATGCCGA CAGAAGCATT AGCTACACCA GGTTACTTTC CTACAGTGGT GCAGCCTTAT 
ATGGAATCAA ATCTTTTGGT TCCTGTGGGC AGTGTAGGAG GACAGGTTCA AGTGTCCCAA 
CCAGCAGTGA GTTTAGCACA AGCCCCCACT GCATCCTCCC AGCAAGCAGC TCTGGAGAGT 
ACTCAGGGAG TCTCTCAGGT TGCTCCTCCA GAGCCAGTTT CGGTAGCACA GCCACAGCCC 
ACCCAGCCTG CCACTTTGGT CTCTTCTATA GATAGTGCAC ATTCAGATGT TGCTTCAGGT 
ATGAGCGATG GCAATGAGAA TGTGCCATCA TCTGGTGGAA GGCATGAAGG GAGAACTGCA 
AAACGGCATT ATCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCACGCCCC 
AAATTGAGAA TTTTGAATGT TTCAAATAAA GGAGACCGGG TAGTAGAATG TCAGTTAGAG 
ACTCACAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCCGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG 
GATCAAGTAC GGGAAATTAT TGAAAAAGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCCGAGG GTGACCAGGG ATTGGAGAGT CTACAGGGAA AGGATGACTA TGGCTTTTCA 
GGTTCTCAGA AATTGGAAGG AGAGTTCAAA CAACCACTTC CTGCATCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTACCAGA AAGCCGATTA CGAGAATCAA AAATTTTCAC CAGTGAAATA 
TCAGATACGG TTGCCGCCTC TACATCTCAT GGCCCTGGGG TGAACTTGTC CCACTCTGCT 
TCTTCCCTTA GTCTACAGCA GGCCTTTTCT GAACTTAGAC ATGCCCAAGT TGCAGAGGGA 
CCCAATACAG CACCTCCCAA CTTCAGTCAT ATAGGACCAA CATTTCCAGT AGTACCTCCT 
TCCATGAGTA GCATTGCTGG AATCCCAACG ACAGCAGCAG CCGTACCTTC AATATCAGTC 
CCTGTAACTA GCTGCCCTCT TAATGACATT TCCACATCAA TAATTCAATC TGAGATTACA 
GTGCCCACTG AAAAAGGAGT TGCTGGAGTT GCCACCTGCA CAGGTGTAAT AACTTCAAGT 
GGTTTCCCAG TACCACCTGC GTCTGAATCA CCAATTCTTT CCAGTGTGGT TTCAAGTATC 
ACAATACCAA CAGTTGTCTC AGTATCAACA ACATCCCAGT CAGTTCAGGC TCCCACATCT 
GGGAGCGTTG TTTCTAGTAT AGGCACTTTC CCTTCTATAC CAGTTTCAGC AACTTCAGCC 
TCTGCAGTGG GCAGTGCTGC TGCCCCAGGG GCTAAGTCTC CACCTTTGTT ATCTGAGCAG 
TCAGCAGGCA TTACTACAGG GATAGCCACA TTAACATCAG TTTCTACCAC CACTCCATTC 
CCAAGCACAG CTTCACAGCT ATCTCTTCAG CTTAGCAGCA GCACCTCTGC TCCTACTCTA 
GCTGAAACAG TGGTGGTTAG TGCACACTCA CTAGATAAAA CATCTCATAG CAGTACAACT 
GGACTGGCTT TGTCCCACTC TGCATCATCT TCCACTTCTT CCCCTGGAGC AGGAGTATCT 
AGTTCTATTT CTCAACCTGG TGGATTACAT CCTTTGGTCA TCCCATCAGC TGTAGTTTCT 
ACTCCTGTCC TTTCCCAAGC ATCAGGACCT ATTTCTACCC CTTTATTACC CCAAGGACCT 
AGCATCCCAC CATTGGTGCA ACCTGTTGCC AATGTGCCTG CTGTACAGCA GACACTAATT 
CATAGTCAGC CTCAACCAGC TTTGCTTCTC AACCAGCCCC ATACGCACTG CCCTGAAATA 
GATGCTGATA CACAACCCAA AGCTCCTGGG ATTGATGACA TAAAGACTCT AGAGGAAAAG 
TTGCGGTCTC TCTTTAGTGA ACACAGCTCA TCTGGCGCTC AACATGCCTC TGTTGCGCTG 
GAGACATCAC TAGTAGTAGA GACCAGTGTC ACACCAGGCA TCCCAACCAC TGCTGTTGCA 
CCCAGCAAAC TCATGACTTC AACTACAAGT ACTTGCTTAC CACCAACCAG TTTGCCATTA 
GGAACAGCTG GTTTGTCCAT TACACCACTG GTCACAACTG GGCAAGTTTC TACCCCAGTC 
AGCACTACAT CAGGAGTGAA ACCTGGAACT GCTCCATCAA AGCTACCTTT AACTAAGGCT 
CCAGTGCTGC CAGTGGGTAC TGAACTTCCA GCTGGTACTC CATCCAGTGA GCAGCTGCCA 
CCTATTCCAG GACCTTCACT AACTCAGTCC CAGCAACCTC TGGAAGATCT TGATGCTCAG 
TTAAGAAGAG CACTTAGTCC AGAGGCTATT GCGGTGACAT CCGCAGTTGG TCCTGTGTCC 
GTGGTGGCTC CAGCGGCAGT TACAGAAGCA GGAGCACAGC CTCAGAAGGA TGTTTCTCAA 
GTCACAGAAG GTTCTGTCCC AGCAGCTAGT TCAGGAACTG GTGTTTTTAA GACGGGGCGA 
TTTCAGGTTT CAGTTGCAAT AGATGATTCC CAGAAAGAGG GTAAAACTAA GTCAGAAGAT 
GCAAAGTCTG TTCATTTTGA ATCCAGCACT TCAGAGTCCT CAGTGCTATC AAGTAGTAGT 
CCAGAGAGTA CCCTGGTGAA GCCAGACTCT AATGGGGTAA CCATCCATGG CATCTCTTCA 
GATATGCCAG ATAGTGCCCA CAAAACTCCT GCCGCAGAAG CAAAGTCAGA AACTGGGCAA 
CCTACCAAGG TTGGGCGTTT TCAGGTGACA ACTACAGCAA ACAAAGTAGG TCGTTTCTCT 
GTATCAAGAA CTGAAGACAG GCTCACTGAG GCAAAGAAAG AGGGACCGAC AGCATCTCCT 
CCTTTTATGG ATTTGGAACA ACCTGTTCTT CCTGCTGCAA TACCAAAGAA AGAAAAGCCT 
GAACTGTCAG AGCCTTCACA TCTGAATGGG CCATCTTCTG ATTTGGAGGC TGCCTTCCTA 
AGTAGGGATG TAGATGATGG TTCAGGTAGT CCACACTCCC CCCACCAACT GAGCTCAAAG 
AGCCTACCTA TCCAGAATCT AAGTCAAAGC CTTAGTAACT CACTCAACTC CTCTTATATG 
AGTAGTGACA ATGAGTCAGA TATTGAAGAT GAAGACTTAA AGTTAGAGCT GCGACGACTA 
CGAGACAAAC ATCTTAAAGA AATTCAAGAC CTGCAGAGTC GCCAGAAGCA TGAAATTGAA 
TCTTTGTATA CTAAACTGGG CAAGGTTCCT CCTGCTGTCA TTATTCCCCC AGCGGCCCCC 
CTTTCAGGAA GAAGAAGGCG ACCCACTAAA AGCAAAGGCA GCAAATCTAG TCGTATCAGT 
TCCTTGGGGA GTAAAAGCCC CCAGCTTTCA GGTAATATGT CTGGTCAGAG TGAAGCTTCA 
GTCTTACACC CCCAGCAGAC CCTCCACCCT CCAGGCAACA TCCCAGAAAC TGGGCAGAAT 
CAGTTGTTAC AGCCCCTTAA GCCATCTCCC TCCAGTGACA ACCTGTATTC AGCCTTCACC 
AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GTCAAGGGAC CAGCAGCACA 
AACACTGTCG GGGGAACAGT GAATAGCCAA GCTGCCCAAG CTCAGCCTCC TGCCATGACG 
TCCAGCAGGA AGGGCACGTT CACAGATGAC CTGCACAAGT TGGTAGACAA TTGGGCCAGA 
GATGCCATGA ATCTTTCAGG CAGGAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT 
GGAATGGCAA GGAAGTTCTC TGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG 
GGTGGCTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA TGGTTTTCCA CCTACCCCCT TTGGCACTCA GTGGAGTGGG 
ACAGGTGGCC CAGCACCACA GCCACTTGGC CAGTTCCAAC CTGTGGGAAC CACCTCCTTA 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG 
CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011366126.1
CDS	667..7818
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHPQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPVSQIPVSTPHVSAAQTGFSTLPITIAAGLNQPLLTLASSATAAAIPGGSTMAPSQLPALLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIAPSSSVASVCIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLLVPVGSVGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPEAIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011367824.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011367824.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCATGGAAT ACAGCAGACA GCCCCTCCTC AACAGACAGT ACAGTATTCA 
CTTCCACAGA CATCAACCTC CAGTGAGGCC ACTACTGCAC AGCCAGTGAG TCAGCCTCAG 
GCTCCACAGG TCTTGCCTCA AGCATCAGCT GGAAAACAGC TTCCAGTTTC CCAGCCAGTA 
CCAACTGTCC AAGGCGAACC TCAGATCTCA GTTGCGACAC AACCCTCAGT TGTTCCAGTG 
CATTCTGGTG CTCATTTCCT CCCTATGGGA CAGCCACTTC CTACTTCCTT ACTCCCTCAG 
TACCCTGTCT CTCAAATTCC TGTATCAACT CCTCATGTGT CCGCGGCTCA GACAGGTTTC 
TCAACCCTTC CCATCACAAT TGCAGCTGGC CTTAATCAGC CTCTGCTCAC CTTGGCTTCA 
TCTGCTACAG CAGCCGCAAT CCCAGGGGGA TCAACTATGG CTCCTAGTCA GCTACCAGCC 
CTTCTGCAGC CTGTGACTCA GCTGCCAAGT CAGGTTCACC CACAGCTCCT GCAGCCAGCA 
GTTCAGTCCA TGGGAATACC AGCTAACCTT GGACAAGCTG CTGAGGTTCC ACTTCCCTCT 
GGAGATGTTC TATACCAGGG CTTCCCACCT CGACTGCCAC CACAATACCC AGGAGATTCA 
AATATTGCTC CCTCTTCCAG CGTGGCTTCT GTTTGCATTC CTTCTACAGT CTTATCCCCT 
CCAATGCCGA CAGAAGCATT AGCTACACCA GGTTACTTTC CTACAGTGGT GCAGCCTTAT 
ATGGAATCAA ATCTTTTGGT TCCTGTGGGC AGTGTAGGAG GACAGGTTCA AGTGTCCCAA 
CCAGCAGTGA GTTTAGCACA AGCCCCCACT GCATCCTCCC AGCAAGCAGC TCTGGAGAGT 
ACTCAGGGAG TCTCTCAGGT TGCTCCTCCA GAGCCAGTTT CGGTAGCACA GCCACAGCCC 
ACCCAGCCTG CCACTTTGGT CTCTTCTATA GATAGTGCAC ATTCAGATGT TGCTTCAGGT 
ATGAGCGATG GCAATGAGAA TGTGCCATCA TCTGGTGGAA GGCATGAAGG GAGAACTGCA 
AAACGGCATT ATCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCACGCCCC 
AAATTGAGAA TTTTGAATGT TTCAAATAAA GGAGACCGGG TAGTAGAATG TCAGTTAGAG 
ACTCACAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCCGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG 
GATCAAGTAC GGGAAATTAT TGAAAAAGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCCGAGG GTGACCAGGG ATTGGAGAGT CTACAGGGAA AGGATGACTA TGGCTTTTCA 
GGTTCTCAGA AATTGGAAGG AGAGTTCAAA CAACCACTTC CTGCATCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTACCAGA AAGCCGATTA CGAGAATCAA AAATTTTCAC CAGTGAAATA 
TCAGATACGG TTGCCGCCTC TACATCTCAT GGCCCTGGGG TGAACTTGTC CCACTCTGCT 
TCTTCCCTTA GTCTACAGCA GGCCTTTTCT GAACTTAGAC ATGCCCAAGT TGCAGAGGGA 
CCCAATACAG CACCTCCCAA CTTCAGTCAT ATAGGACCAA CATTTCCAGT AGTACCTCCT 
TCCATGAGTA GCATTGCTGG AATCCCAACG ACAGCAGCAG CCGTACCTTC AATATCAGTC 
CCTGTAACTA GCTGCCCTCT TAATGACATT TCCACATCAA TAATTCAATC TGAGATTACA 
GTGCCCACTG AAAAAGGAGT TGCTGGAGTT GCCACCTGCA CAGGTGTAAT AACTTCAAGT 
GGTTTCCCAG TACCACCTGC GTCTGAATCA CCAATTCTTT CCAGTGTGGT TTCAAGTATC 
ACAATACCAA CAGTTGTCTC AGTATCAACA ACATCCCAGT CAGTTCAGGC TCCCACATCT 
GGGAGCGTTG TTTCTAGTAT AGGCACTTTC CCTTCTATAC CAGTTTCAGC AACTTCAGCC 
TCTGCAGTGG GCAGTGCTGC TGCCCCAGGG GCTAAGTCTC CACCTTTGTT ATCTGAGCAG 
TCAGCAGGCA TTACTACAGG GATAGCCACA TTAACATCAG TTTCTACCAC CACTCCATTC 
CCAAGCACAG CTTCACAGCT ATCTCTTCAG CTTAGCAGCA GCACCTCTGC TCCTACTCTA 
GCTGAAACAG TGGTGGTTAG TGCACACTCA CTAGATAAAA CATCTCATAG CAGTACAACT 
GGACTGGCTT TGTCCCACTC TGCATCATCT TCCACTTCTT CCCCTGGAGC AGGAGTATCT 
AGTTCTATTT CTCAACCTGG TGGATTACAT CCTTTGGTCA TCCCATCAGC TGTAGTTTCT 
ACTCCTGTCC TTTCCCAAGC ATCAGGACCT ATTTCTACCC CTTTATTACC CCAAGGACCT 
AGCATCCCAC CATTGGTGCA ACCTGTTGCC AATGTGCCTG CTGTACAGCA GACACTAATT 
CATAGTCAGC CTCAACCAGC TTTGCTTCTC AACCAGCCCC ATACGCACTG CCCTGAAATA 
GATGCTGATA CACAACCCAA AGCTCCTGGG ATTGATGACA TAAAGACTCT AGAGGAAAAG 
TTGCGGTCTC TCTTTAGTGA ACACAGCTCA TCTGGCGCTC AACATGCCTC TGTTGCGCTG 
GAGACATCAC TAGTAGTAGA GACCAGTGTC ACACCAGGCA TCCCAACCAC TGCTGTTGCA 
CCCAGCAAAC TCATGACTTC AACTACAAGT ACTTGCTTAC CACCAACCAG TTTGCCATTA 
GGAACAGCTG GTTTGTCCAT TACACCACTG GTCACAACTG GGCAAGTTTC TACCCCAGTC 
AGCACTACAT CAGGAGTGAA ACCTGGAACT GCTCCATCAA AGCTACCTTT AACTAAGGCT 
CCAGTGCTGC CAGTGGGTAC TGAACTTCCA GCTGGTACTC CATCCAGTGA GCAGCTGCCA 
CCTATTCCAG GACCTTCACT AACTCAGTCC CAGCAACCTC TGGAAGATCT TGATGCTCAG 
TTAAGAAGAG CACTTAGTCC AGAGGCTATT GCGGTGACAT CCGCAGTTGG TCCTGTGTCC 
GTGGTGGCTC CAGCGGCAGT TACAGAAGCA GGAGCACAGC CTCAGAAGGA TGTTTCTCAA 
GTCACAGAAG GTTCTGTCCC AGCAGCTAGT TCAGGAACTG GTGTTTTTAA GACGGGGCGA 
TTTCAGGTTT CAGTTGCAAT AGATGATTCC CAGAAAGAGG GTAAAACTAA GTCAGAAGAT 
GCAAAGTCTG TTCATTTTGA ATCCAGCACT TCAGAGTCCT CAGTGCTATC AAGTAGTAGT 
CCAGAGAGTA CCCTGGTGAA GCCAGACTCT AATGGGGTAA CCATCCATGG CATCTCTTCA 
GATATGCCAG ATAGTGCCCA CAAAACTCCT GCCGCAGAAG CAAAGTCAGA AACTGGGCAA 
CCTACCAAGG TTGGGCGTTT TCAGGTGACA ACTACAGCAA ACAAAGTAGG TCGTTTCTCT 
GTATCAAGAA CTGAAGACAG GCTCACTGAG GCAAAGAAAG AGGGACCGAC AGCATCTCCT 
CCTTTTATGG ATTTGGAACA ACCTGTTCTT CCTGCTGCAA TACCAAAGAA AGAAAAGCCT 
GAACTGTCAG AGCCTTCACA TCTGAATGGG CCATCTTCTG ATTTGGAGGC TGCCTTCCTA 
AGTAGGGATG TAGATGATGG TTCAGGTAGT CCACACTCCC CCCACCAACT GAGCTCAAAG 
AGCCTACCTA TCCAGAATCT AAGTCAAAGC CTTAGTAACT CACTCAACTC CTCTTATATG 
AGTAGTGACA ATGAGTCAGA TATTGAAGAT GAAGACTTAA AGTTAGAGCT GCGACGACTA 
CGAGACAAAC ATCTTAAAGA AATTCAAGAC CTGCAGAGTC GCCAGAAGCA TGAAATTGAA 
TCTTTGTATA CTAAACTGGG CAAGGTTCCT CCTGCTGTCA TTATTCCCCC AGCGGCCCCC 
CTTTCAGGAA GAAGAAGGCG ACCCACTAAA AGCAAAGGCA GCAAATCTAG TCGTATCAGT 
TCCTTGGGGA GTAAAAGCCC CCAGCTTTCA GGTAATATGT CTGGTCAGAG TGAAGCTTCA 
GTCTTACACC CCCAGCAGAC CCTCCACCCT CCAGGCAACA TCCCAGAAAC TGGGCAGAAT 
CAGTTGTTAC AGCCCCTTAA GCCATCTCCC TCCAGTGACA ACCTGTATTC AGCCTTCACC 
AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GTCAAGGGAC CAGCAGCACA 
AACACTGTCG GGGGAACAGT GAATAGCCAA GCTGCCCAAG CTCAGCCTCC TGCCATGACG 
TCCAGCAGGA AGGGCACGTT CACAGATGAC CTGCACAAGT TGGTAGACAA TTGGGCCAGA 
GATGCCATGA ATCTTTCAGG CAGGAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT 
GGAATGGCAA GGAAGTTCTC TGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG 
GGTGGCTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA TGGTTTTCCA CCTACCCCCT TTGGCACTCA GTGGAGTGGG 
ACAGGTGGCC CAGCACCACA GCCACTTGGC CAGTTCCAAC CTGTGGGAAC CACCTCCTTA 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG 
CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011367824.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011366126.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

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CloneID	OPt52295
Clone ID Related Accession (Same CDS sequence)	XM_011367824.2 , XM_011367824.1
Accession Version	XM_011367824.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCATGGAAT ACAGCAGACA GCCCCTCCTC AACAGACAGT ACAGTATTCA 
CTTCCACAGA CATCAACCTC CAGTGAGGCC ACTACTGCAC AGCCAGTGAG TCAGCCTCAG 
GCTCCACAGG TCTTGCCTCA AGCATCAGCT GGAAAACAGC TTCCAGTTTC CCAGCCAGTA 
CCAACTGTCC AAGGCGAACC TCAGATCTCA GTTGCGACAC AACCCTCAGT TGTTCCAGTG 
CATTCTGGTG CTCATTTCCT CCCTATGGGA CAGCCACTTC CTACTTCCTT ACTCCCTCAG 
TACCCTGTCT CTCAAATTCC TGTATCAACT CCTCATGTGT CCGCGGCTCA GACAGGTTTC 
TCAACCCTTC CCATCACAAT TGCAGCTGGC CTTAATCAGC CTCTGCTCAC CTTGGCTTCA 
TCTGCTACAG CAGCCGCAAT CCCAGGGGGA TCAACTATGG CTCCTAGTCA GCTACCAGCC 
CTTCTGCAGC CTGTGACTCA GCTGCCAAGT CAGGTTCACC CACAGCTCCT GCAGCCAGCA 
GTTCAGTCCA TGGGAATACC AGCTAACCTT GGACAAGCTG CTGAGGTTCC ACTTCCCTCT 
GGAGATGTTC TATACCAGGG CTTCCCACCT CGACTGCCAC CACAATACCC AGGAGATTCA 
AATATTGCTC CCTCTTCCAG CGTGGCTTCT GTTTGCATTC CTTCTACAGT CTTATCCCCT 
CCAATGCCGA CAGAAGCATT AGCTACACCA GGTTACTTTC CTACAGTGGT GCAGCCTTAT 
ATGGAATCAA ATCTTTTGGT TCCTGTGGGC AGTGTAGGAG GACAGGTTCA AGTGTCCCAA 
CCAGCAGTGA GTTTAGCACA AGCCCCCACT GCATCCTCCC AGCAAGCAGC TCTGGAGAGT 
ACTCAGGGAG TCTCTCAGGT TGCTCCTCCA GAGCCAGTTT CGGTAGCACA GCCACAGCCC 
ACCCAGCCTG CCACTTTGGT CTCTTCTATA GATAGTGCAC ATTCAGATGT TGCTTCAGGT 
ATGAGCGATG GCAATGAGAA TGTGCCATCA TCTGGTGGAA GGCATGAAGG GAGAACTGCA 
AAACGGCATT ATCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCACGCCCC 
AAATTGAGAA TTTTGAATGT TTCAAATAAA GGAGACCGGG TAGTAGAATG TCAGTTAGAG 
ACTCACAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCCGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG 
GATCAAGTAC GGGAAATTAT TGAAAAAGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCCGAGG GTGACCAGGG ATTGGAGAGT CTACAGGGAA AGGATGACTA TGGCTTTTCA 
GGTTCTCAGA AATTGGAAGG AGAGTTCAAA CAACCACTTC CTGCATCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTACCAGA AAGCCGATTA CGAGAATCAA AAATTTTCAC CAGTGAAATA 
TCAGATACGG TTGCCGCCTC TACATCTCAT GGCCCTGGGG TGAACTTGTC CCACTCTGCT 
TCTTCCCTTA GTCTACAGCA GGCCTTTTCT GAACTTAGAC ATGCCCAAGT TGCAGAGGGA 
CCCAATACAG CACCTCCCAA CTTCAGTCAT ATAGGACCAA CATTTCCAGT AGTACCTCCT 
TCCATGAGTA GCATTGCTGG AATCCCAACG ACAGCAGCAG CCGTACCTTC AATATCAGTC 
CCTGTAACTA GCTGCCCTCT TAATGACATT TCCACATCAA TAATTCAATC TGAGATTACA 
GTGCCCACTG AAAAAGGAGT TGCTGGAGTT GCCACCTGCA CAGGTGTAAT AACTTCAAGT 
GGTTTCCCAG TACCACCTGC GTCTGAATCA CCAATTCTTT CCAGTGTGGT TTCAAGTATC 
ACAATACCAA CAGTTGTCTC AGTATCAACA ACATCCCAGT CAGTTCAGGC TCCCACATCT 
GGGAGCGTTG TTTCTAGTAT AGGCACTTTC CCTTCTATAC CAGTTTCAGC AACTTCAGCC 
TCTGCAGTGG GCAGTGCTGC TGCCCCAGGG GCTAAGTCTC CACCTTTGTT ATCTGAGCAG 
TCAGCAGGCA TTACTACAGG GATAGCCACA TTAACATCAG TTTCTACCAC CACTCCATTC 
CCAAGCACAG CTTCACAGCT ATCTCTTCAG CTTAGCAGCA GCACCTCTGC TCCTACTCTA 
GCTGAAACAG TGGTGGTTAG TGCACACTCA CTAGATAAAA CATCTCATAG CAGTACAACT 
GGACTGGCTT TGTCCCACTC TGCATCATCT TCCACTTCTT CCCCTGGAGC AGGAGTATCT 
AGTTCTATTT CTCAACCTGG TGGATTACAT CCTTTGGTCA TCCCATCAGC TGTAGTTTCT 
ACTCCTGTCC TTTCCCAAGC ATCAGGACCT ATTTCTACCC CTTTATTACC CCAAGGACCT 
AGCATCCCAC CATTGGTGCA ACCTGTTGCC AATGTGCCTG CTGTACAGCA GACACTAATT 
CATAGTCAGC CTCAACCAGC TTTGCTTCTC AACCAGCCCC ATACGCACTG CCCTGAAATA 
GATGCTGATA CACAACCCAA AGCTCCTGGG ATTGATGACA TAAAGACTCT AGAGGAAAAG 
TTGCGGTCTC TCTTTAGTGA ACACAGCTCA TCTGGCGCTC AACATGCCTC TGTTGCGCTG 
GAGACATCAC TAGTAGTAGA GACCAGTGTC ACACCAGGCA TCCCAACCAC TGCTGTTGCA 
CCCAGCAAAC TCATGACTTC AACTACAAGT ACTTGCTTAC CACCAACCAG TTTGCCATTA 
GGAACAGCTG GTTTGTCCAT TACACCACTG GTCACAACTG GGCAAGTTTC TACCCCAGTC 
AGCACTACAT CAGGAGTGAA ACCTGGAACT GCTCCATCAA AGCTACCTTT AACTAAGGCT 
CCAGTGCTGC CAGTGGGTAC TGAACTTCCA GCTGGTACTC CATCCAGTGA GCAGCTGCCA 
CCTATTCCAG GACCTTCACT AACTCAGTCC CAGCAACCTC TGGAAGATCT TGATGCTCAG 
TTAAGAAGAG CACTTAGTCC AGAGGCTATT GCGGTGACAT CCGCAGTTGG TCCTGTGTCC 
GTGGTGGCTC CAGCGGCAGT TACAGAAGCA GGAGCACAGC CTCAGAAGGA TGTTTCTCAA 
GTCACAGAAG GTTCTGTCCC AGCAGCTAGT TCAGGAACTG GTGTTTTTAA GACGGGGCGA 
TTTCAGGTTT CAGTTGCAAT AGATGATTCC CAGAAAGAGG GTAAAACTAA GTCAGAAGAT 
GCAAAGTCTG TTCATTTTGA ATCCAGCACT TCAGAGTCCT CAGTGCTATC AAGTAGTAGT 
CCAGAGAGTA CCCTGGTGAA GCCAGACTCT AATGGGGTAA CCATCCATGG CATCTCTTCA 
GATATGCCAG ATAGTGCCCA CAAAACTCCT GCCGCAGAAG CAAAGTCAGA AACTGGGCAA 
CCTACCAAGG TTGGGCGTTT TCAGGTGACA ACTACAGCAA ACAAAGTAGG TCGTTTCTCT 
GTATCAAGAA CTGAAGACAG GCTCACTGAG GCAAAGAAAG AGGGACCGAC AGCATCTCCT 
CCTTTTATGG ATTTGGAACA ACCTGTTCTT CCTGCTGCAA TACCAAAGAA AGAAAAGCCT 
GAACTGTCAG AGCCTTCACA TCTGAATGGG CCATCTTCTG ATTTGGAGGC TGCCTTCCTA 
AGTAGGGATG TAGATGATGG TTCAGGTAGT CCACACTCCC CCCACCAACT GAGCTCAAAG 
AGCCTACCTA TCCAGAATCT AAGTCAAAGC CTTAGTAACT CACTCAACTC CTCTTATATG 
AGTAGTGACA ATGAGTCAGA TATTGAAGAT GAAGACTTAA AGTTAGAGCT GCGACGACTA 
CGAGACAAAC ATCTTAAAGA AATTCAAGAC CTGCAGAGTC GCCAGAAGCA TGAAATTGAA 
TCTTTGTATA CTAAACTGGG CAAGGTTCCT CCTGCTGTCA TTATTCCCCC AGCGGCCCCC 
CTTTCAGGAA GAAGAAGGCG ACCCACTAAA AGCAAAGGCA GCAAATCTAG TCGTATCAGT 
TCCTTGGGGA GTAAAAGCCC CCAGCTTTCA GGTAATATGT CTGGTCAGAG TGAAGCTTCA 
GTCTTACACC CCCAGCAGAC CCTCCACCCT CCAGGCAACA TCCCAGAAAC TGGGCAGAAT 
CAGTTGTTAC AGCCCCTTAA GCCATCTCCC TCCAGTGACA ACCTGTATTC AGCCTTCACC 
AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GTCAAGGGAC CAGCAGCACA 
AACACTGTCG GGGGAACAGT GAATAGCCAA GCTGCCCAAG CTCAGCCTCC TGCCATGACG 
TCCAGCAGGA AGGGCACGTT CACAGATGAC CTGCACAAGT TGGTAGACAA TTGGGCCAGA 
GATGCCATGA ATCTTTCAGG CAGGAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT 
GGAATGGCAA GGAAGTTCTC TGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG 
GGTGGCTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA TGGTTTTCCA CCTACCCCCT TTGGCACTCA GTGGAGTGGG 
ACAGGTGGCC CAGCACCACA GCCACTTGGC CAGTTCCAAC CTGTGGGAAC CACCTCCTTA 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG 
CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011366126.1
CDS	1012..8163
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHPQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPVSQIPVSTPHVSAAQTGFSTLPITIAAGLNQPLLTLASSATAAAIPGGSTMAPSQLPALLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIAPSSSVASVCIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLLVPVGSVGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPEAIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011367824.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011367824.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCATGGAAT ACAGCAGACA GCCCCTCCTC AACAGACAGT ACAGTATTCA 
CTTCCACAGA CATCAACCTC CAGTGAGGCC ACTACTGCAC AGCCAGTGAG TCAGCCTCAG 
GCTCCACAGG TCTTGCCTCA AGCATCAGCT GGAAAACAGC TTCCAGTTTC CCAGCCAGTA 
CCAACTGTCC AAGGCGAACC TCAGATCTCA GTTGCGACAC AACCCTCAGT TGTTCCAGTG 
CATTCTGGTG CTCATTTCCT CCCTATGGGA CAGCCACTTC CTACTTCCTT ACTCCCTCAG 
TACCCTGTCT CTCAAATTCC TGTATCAACT CCTCATGTGT CCGCGGCTCA GACAGGTTTC 
TCAACCCTTC CCATCACAAT TGCAGCTGGC CTTAATCAGC CTCTGCTCAC CTTGGCTTCA 
TCTGCTACAG CAGCCGCAAT CCCAGGGGGA TCAACTATGG CTCCTAGTCA GCTACCAGCC 
CTTCTGCAGC CTGTGACTCA GCTGCCAAGT CAGGTTCACC CACAGCTCCT GCAGCCAGCA 
GTTCAGTCCA TGGGAATACC AGCTAACCTT GGACAAGCTG CTGAGGTTCC ACTTCCCTCT 
GGAGATGTTC TATACCAGGG CTTCCCACCT CGACTGCCAC CACAATACCC AGGAGATTCA 
AATATTGCTC CCTCTTCCAG CGTGGCTTCT GTTTGCATTC CTTCTACAGT CTTATCCCCT 
CCAATGCCGA CAGAAGCATT AGCTACACCA GGTTACTTTC CTACAGTGGT GCAGCCTTAT 
ATGGAATCAA ATCTTTTGGT TCCTGTGGGC AGTGTAGGAG GACAGGTTCA AGTGTCCCAA 
CCAGCAGTGA GTTTAGCACA AGCCCCCACT GCATCCTCCC AGCAAGCAGC TCTGGAGAGT 
ACTCAGGGAG TCTCTCAGGT TGCTCCTCCA GAGCCAGTTT CGGTAGCACA GCCACAGCCC 
ACCCAGCCTG CCACTTTGGT CTCTTCTATA GATAGTGCAC ATTCAGATGT TGCTTCAGGT 
ATGAGCGATG GCAATGAGAA TGTGCCATCA TCTGGTGGAA GGCATGAAGG GAGAACTGCA 
AAACGGCATT ATCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCACGCCCC 
AAATTGAGAA TTTTGAATGT TTCAAATAAA GGAGACCGGG TAGTAGAATG TCAGTTAGAG 
ACTCACAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCCGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG 
GATCAAGTAC GGGAAATTAT TGAAAAAGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCCGAGG GTGACCAGGG ATTGGAGAGT CTACAGGGAA AGGATGACTA TGGCTTTTCA 
GGTTCTCAGA AATTGGAAGG AGAGTTCAAA CAACCACTTC CTGCATCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTACCAGA AAGCCGATTA CGAGAATCAA AAATTTTCAC CAGTGAAATA 
TCAGATACGG TTGCCGCCTC TACATCTCAT GGCCCTGGGG TGAACTTGTC CCACTCTGCT 
TCTTCCCTTA GTCTACAGCA GGCCTTTTCT GAACTTAGAC ATGCCCAAGT TGCAGAGGGA 
CCCAATACAG CACCTCCCAA CTTCAGTCAT ATAGGACCAA CATTTCCAGT AGTACCTCCT 
TCCATGAGTA GCATTGCTGG AATCCCAACG ACAGCAGCAG CCGTACCTTC AATATCAGTC 
CCTGTAACTA GCTGCCCTCT TAATGACATT TCCACATCAA TAATTCAATC TGAGATTACA 
GTGCCCACTG AAAAAGGAGT TGCTGGAGTT GCCACCTGCA CAGGTGTAAT AACTTCAAGT 
GGTTTCCCAG TACCACCTGC GTCTGAATCA CCAATTCTTT CCAGTGTGGT TTCAAGTATC 
ACAATACCAA CAGTTGTCTC AGTATCAACA ACATCCCAGT CAGTTCAGGC TCCCACATCT 
GGGAGCGTTG TTTCTAGTAT AGGCACTTTC CCTTCTATAC CAGTTTCAGC AACTTCAGCC 
TCTGCAGTGG GCAGTGCTGC TGCCCCAGGG GCTAAGTCTC CACCTTTGTT ATCTGAGCAG 
TCAGCAGGCA TTACTACAGG GATAGCCACA TTAACATCAG TTTCTACCAC CACTCCATTC 
CCAAGCACAG CTTCACAGCT ATCTCTTCAG CTTAGCAGCA GCACCTCTGC TCCTACTCTA 
GCTGAAACAG TGGTGGTTAG TGCACACTCA CTAGATAAAA CATCTCATAG CAGTACAACT 
GGACTGGCTT TGTCCCACTC TGCATCATCT TCCACTTCTT CCCCTGGAGC AGGAGTATCT 
AGTTCTATTT CTCAACCTGG TGGATTACAT CCTTTGGTCA TCCCATCAGC TGTAGTTTCT 
ACTCCTGTCC TTTCCCAAGC ATCAGGACCT ATTTCTACCC CTTTATTACC CCAAGGACCT 
AGCATCCCAC CATTGGTGCA ACCTGTTGCC AATGTGCCTG CTGTACAGCA GACACTAATT 
CATAGTCAGC CTCAACCAGC TTTGCTTCTC AACCAGCCCC ATACGCACTG CCCTGAAATA 
GATGCTGATA CACAACCCAA AGCTCCTGGG ATTGATGACA TAAAGACTCT AGAGGAAAAG 
TTGCGGTCTC TCTTTAGTGA ACACAGCTCA TCTGGCGCTC AACATGCCTC TGTTGCGCTG 
GAGACATCAC TAGTAGTAGA GACCAGTGTC ACACCAGGCA TCCCAACCAC TGCTGTTGCA 
CCCAGCAAAC TCATGACTTC AACTACAAGT ACTTGCTTAC CACCAACCAG TTTGCCATTA 
GGAACAGCTG GTTTGTCCAT TACACCACTG GTCACAACTG GGCAAGTTTC TACCCCAGTC 
AGCACTACAT CAGGAGTGAA ACCTGGAACT GCTCCATCAA AGCTACCTTT AACTAAGGCT 
CCAGTGCTGC CAGTGGGTAC TGAACTTCCA GCTGGTACTC CATCCAGTGA GCAGCTGCCA 
CCTATTCCAG GACCTTCACT AACTCAGTCC CAGCAACCTC TGGAAGATCT TGATGCTCAG 
TTAAGAAGAG CACTTAGTCC AGAGGCTATT GCGGTGACAT CCGCAGTTGG TCCTGTGTCC 
GTGGTGGCTC CAGCGGCAGT TACAGAAGCA GGAGCACAGC CTCAGAAGGA TGTTTCTCAA 
GTCACAGAAG GTTCTGTCCC AGCAGCTAGT TCAGGAACTG GTGTTTTTAA GACGGGGCGA 
TTTCAGGTTT CAGTTGCAAT AGATGATTCC CAGAAAGAGG GTAAAACTAA GTCAGAAGAT 
GCAAAGTCTG TTCATTTTGA ATCCAGCACT TCAGAGTCCT CAGTGCTATC AAGTAGTAGT 
CCAGAGAGTA CCCTGGTGAA GCCAGACTCT AATGGGGTAA CCATCCATGG CATCTCTTCA 
GATATGCCAG ATAGTGCCCA CAAAACTCCT GCCGCAGAAG CAAAGTCAGA AACTGGGCAA 
CCTACCAAGG TTGGGCGTTT TCAGGTGACA ACTACAGCAA ACAAAGTAGG TCGTTTCTCT 
GTATCAAGAA CTGAAGACAG GCTCACTGAG GCAAAGAAAG AGGGACCGAC AGCATCTCCT 
CCTTTTATGG ATTTGGAACA ACCTGTTCTT CCTGCTGCAA TACCAAAGAA AGAAAAGCCT 
GAACTGTCAG AGCCTTCACA TCTGAATGGG CCATCTTCTG ATTTGGAGGC TGCCTTCCTA 
AGTAGGGATG TAGATGATGG TTCAGGTAGT CCACACTCCC CCCACCAACT GAGCTCAAAG 
AGCCTACCTA TCCAGAATCT AAGTCAAAGC CTTAGTAACT CACTCAACTC CTCTTATATG 
AGTAGTGACA ATGAGTCAGA TATTGAAGAT GAAGACTTAA AGTTAGAGCT GCGACGACTA 
CGAGACAAAC ATCTTAAAGA AATTCAAGAC CTGCAGAGTC GCCAGAAGCA TGAAATTGAA 
TCTTTGTATA CTAAACTGGG CAAGGTTCCT CCTGCTGTCA TTATTCCCCC AGCGGCCCCC 
CTTTCAGGAA GAAGAAGGCG ACCCACTAAA AGCAAAGGCA GCAAATCTAG TCGTATCAGT 
TCCTTGGGGA GTAAAAGCCC CCAGCTTTCA GGTAATATGT CTGGTCAGAG TGAAGCTTCA 
GTCTTACACC CCCAGCAGAC CCTCCACCCT CCAGGCAACA TCCCAGAAAC TGGGCAGAAT 
CAGTTGTTAC AGCCCCTTAA GCCATCTCCC TCCAGTGACA ACCTGTATTC AGCCTTCACC 
AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GTCAAGGGAC CAGCAGCACA 
AACACTGTCG GGGGAACAGT GAATAGCCAA GCTGCCCAAG CTCAGCCTCC TGCCATGACG 
TCCAGCAGGA AGGGCACGTT CACAGATGAC CTGCACAAGT TGGTAGACAA TTGGGCCAGA 
GATGCCATGA ATCTTTCAGG CAGGAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT 
GGAATGGCAA GGAAGTTCTC TGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG 
GGTGGCTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA TGGTTTTCCA CCTACCCCCT TTGGCACTCA GTGGAGTGGG 
ACAGGTGGCC CAGCACCACA GCCACTTGGC CAGTTCCAAC CTGTGGGAAC CACCTCCTTA 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG 
CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011367825.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011366127.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

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CloneID	OPt52296
Clone ID Related Accession (Same CDS sequence)	XM_011367825.2 , XM_011367825.1
Accession Version	XM_011367825.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011367825.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG TCTCTCAAAT TCCTGTATCA ACTCCTCATG TGTCCGCGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCCTTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCCGC AATCCCAGGG GGATCAACTA TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT CCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTCCCTCTTC CAGCGTGGCT TCTGTTTGCA TTCCTTCTAC AGTCTTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTTT GGTTCCTGTG GGCAGTGTAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCACTTT GGTCTCTTCT ATAGATAGTG CACATTCAGA TGTTGCTTCA 
GGTATGAGCG ATGGCAATGA GAATGTGCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGTCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAGTTA 
GAGACTCACA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCCGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCGTTT 
GTGGATCAAG TACGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCCG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA AGGAGAGTTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GCATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC TGGAAGACGG 
TTTATAGTGA GTCCTGTACC AGAAAGCCGA TTACGAGAAT CAAAAATTTT CACCAGTGAA 
ATATCAGATA CGGTTGCCGC CTCTACATCT CATGGCCCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTTA GACATGCCCA AGTTGCAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATATAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAATCCCA ACGACAGCAG CAGCCGTACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC ATTTCCACAT CAATAATTCA ATCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCACC TGCGTCTGAA TCACCAATTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CAACAGTTGT CTCAGTATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TATAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCAGTGC TGCTGCCCCA GGGGCTAAGT CTCCACCTTT GTTATCTGAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCACTCCA 
TTCCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCACTAGATA AAACATCTCA TAGCAGTACA 
ACTGGACTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAACC TGGTGGATTA CATCCTTTGG TCATCCCATC AGCTGTAGTT 
TCTACTCCTG TCCTTTCCCA AGCATCAGGA CCTATTTCTA CCCCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CTCAACCAGC CCCATACGCA CTGCCCTGAA 
ATAGATGCTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TCTAGAGGAA 
AAGTTGCGGT CTCTCTTTAG TGAACACAGC TCATCTGGCG CTCAACATGC CTCTGTTGCG 
CTGGAGACAT CACTAGTAGT AGAGACCAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CATTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCA 
GTCAGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG CCTGTGTCCG TGGTGGCTCC AGCGGCAGTT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCACAGAAGG TTCTGTCCCA 
GCAGCTAGTT CAGGAACTGG TGTTTTTAAG ACGGGGCGAT TTCAGGTTTC AGTTGCAATA 
GATGATTCCC AGAAAGAGGG TAAAACTAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CCTGGTGAAG 
CCAGACTCTA ATGGGGTAAC CATCCATGGC ATCTCTTCAG ATATGCCAGA TAGTGCCCAC 
AAAACTCCTG CCGCAGAAGC AAAGTCAGAA ACTGGGCAAC CTACCAAGGT TGGGCGTTTT 
CAGGTGACAA CTACAGCAAA CAAAGTAGGT CGTTTCTCTG TATCAAGAAC TGAAGACAGG 
CTCACTGAGG CAAAGAAAGA GGGACCGACA GCATCTCCTC CTTTTATGGA TTTGGAACAA 
CCTGTTCTTC CTGCTGCAAT ACCAAAGAAA GAAAAGCCTG AACTGTCAGA GCCTTCACAT 
CTGAATGGGC CATCTTCTGA TTTGGAGGCT GCCTTCCTAA GTAGGGATGT AGATGATGGT 
TCAGGTAGTC CACACTCCCC CCACCAACTG AGCTCAAAGA GCCTACCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAACTC ACTCAACTCC TCTTATATGA GTAGTGACAA TGAGTCAGAT 
ATTGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGACAAACA TCTTAAAGAA 
ATTCAAGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC TAAACTGGGC 
AAGGTTCCTC CTGCTGTCAT TATTCCCCCA GCGGCCCCCC TTTCAGGAAG AAGAAGGCGA 
CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGTATCAGTT CCTTGGGGAG TAAAAGCCCC 
CAGCTTTCAG GTAATATGTC TGGTCAGAGT GAAGCTTCAG TCTTACACCC CCAGCAGACC 
CTCCACCCTC CAGGCAACAT CCCAGAAACT GGGCAGAATC AGTTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTGTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG TCAAGGGACC AGCAGCACAA ACACTGTCGG GGGAACAGTG 
AATAGCCAAG CTGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACGTTC 
ACAGATGACC TGCACAAGTT GGTAGACAAT TGGGCCAGAG ATGCCATGAA TCTTTCAGGC 
AGGAGAGGAA GCAAAGGACA CATGAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTCTCT 
GCACCTGGTC AGCTGTGCAT CTCCATGACC TCAAATCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAT 
GGTTTTCCAC CTACCCCCTT TGGCACTCAG TGGAGTGGGA CAGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACC ACCTCCTTAC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011366127.1
CDS	666..7736
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHPQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPVSQIPVSTPHVSAAQTGFSTLPITIAAGLNQPLLTLASSATAAAIPGGSTMAPSQLPALLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIAPSSSVASVCIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLLVPVGSVGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011367825.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011367825.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG TCTCTCAAAT TCCTGTATCA ACTCCTCATG TGTCCGCGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCCTTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCCGC AATCCCAGGG GGATCAACTA TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT CCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTCCCTCTTC CAGCGTGGCT TCTGTTTGCA TTCCTTCTAC AGTCTTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTTT GGTTCCTGTG GGCAGTGTAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCACTTT GGTCTCTTCT ATAGATAGTG CACATTCAGA TGTTGCTTCA 
GGTATGAGCG ATGGCAATGA GAATGTGCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGTCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAGTTA 
GAGACTCACA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCCGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCGTTT 
GTGGATCAAG TACGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCCG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA AGGAGAGTTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GCATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC TGGAAGACGG 
TTTATAGTGA GTCCTGTACC AGAAAGCCGA TTACGAGAAT CAAAAATTTT CACCAGTGAA 
ATATCAGATA CGGTTGCCGC CTCTACATCT CATGGCCCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTTA GACATGCCCA AGTTGCAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATATAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAATCCCA ACGACAGCAG CAGCCGTACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC ATTTCCACAT CAATAATTCA ATCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCACC TGCGTCTGAA TCACCAATTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CAACAGTTGT CTCAGTATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TATAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCAGTGC TGCTGCCCCA GGGGCTAAGT CTCCACCTTT GTTATCTGAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCACTCCA 
TTCCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCACTAGATA AAACATCTCA TAGCAGTACA 
ACTGGACTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAACC TGGTGGATTA CATCCTTTGG TCATCCCATC AGCTGTAGTT 
TCTACTCCTG TCCTTTCCCA AGCATCAGGA CCTATTTCTA CCCCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CTCAACCAGC CCCATACGCA CTGCCCTGAA 
ATAGATGCTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TCTAGAGGAA 
AAGTTGCGGT CTCTCTTTAG TGAACACAGC TCATCTGGCG CTCAACATGC CTCTGTTGCG 
CTGGAGACAT CACTAGTAGT AGAGACCAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CATTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCA 
GTCAGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG CCTGTGTCCG TGGTGGCTCC AGCGGCAGTT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCACAGAAGG TTCTGTCCCA 
GCAGCTAGTT CAGGAACTGG TGTTTTTAAG ACGGGGCGAT TTCAGGTTTC AGTTGCAATA 
GATGATTCCC AGAAAGAGGG TAAAACTAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CCTGGTGAAG 
CCAGACTCTA ATGGGGTAAC CATCCATGGC ATCTCTTCAG ATATGCCAGA TAGTGCCCAC 
AAAACTCCTG CCGCAGAAGC AAAGTCAGAA ACTGGGCAAC CTACCAAGGT TGGGCGTTTT 
CAGGTGACAA CTACAGCAAA CAAAGTAGGT CGTTTCTCTG TATCAAGAAC TGAAGACAGG 
CTCACTGAGG CAAAGAAAGA GGGACCGACA GCATCTCCTC CTTTTATGGA TTTGGAACAA 
CCTGTTCTTC CTGCTGCAAT ACCAAAGAAA GAAAAGCCTG AACTGTCAGA GCCTTCACAT 
CTGAATGGGC CATCTTCTGA TTTGGAGGCT GCCTTCCTAA GTAGGGATGT AGATGATGGT 
TCAGGTAGTC CACACTCCCC CCACCAACTG AGCTCAAAGA GCCTACCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAACTC ACTCAACTCC TCTTATATGA GTAGTGACAA TGAGTCAGAT 
ATTGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGACAAACA TCTTAAAGAA 
ATTCAAGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC TAAACTGGGC 
AAGGTTCCTC CTGCTGTCAT TATTCCCCCA GCGGCCCCCC TTTCAGGAAG AAGAAGGCGA 
CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGTATCAGTT CCTTGGGGAG TAAAAGCCCC 
CAGCTTTCAG GTAATATGTC TGGTCAGAGT GAAGCTTCAG TCTTACACCC CCAGCAGACC 
CTCCACCCTC CAGGCAACAT CCCAGAAACT GGGCAGAATC AGTTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTGTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG TCAAGGGACC AGCAGCACAA ACACTGTCGG GGGAACAGTG 
AATAGCCAAG CTGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACGTTC 
ACAGATGACC TGCACAAGTT GGTAGACAAT TGGGCCAGAG ATGCCATGAA TCTTTCAGGC 
AGGAGAGGAA GCAAAGGACA CATGAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTCTCT 
GCACCTGGTC AGCTGTGCAT CTCCATGACC TCAAATCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAT 
GGTTTTCCAC CTACCCCCTT TGGCACTCAG TGGAGTGGGA CAGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACC ACCTCCTTAC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011367825.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011366127.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

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CloneID	OPt52296
Clone ID Related Accession (Same CDS sequence)	XM_011367825.2 , XM_011367825.1
Accession Version	XM_011367825.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG TCTCTCAAAT TCCTGTATCA ACTCCTCATG TGTCCGCGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCCTTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCCGC AATCCCAGGG GGATCAACTA TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT CCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTCCCTCTTC CAGCGTGGCT TCTGTTTGCA TTCCTTCTAC AGTCTTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTTT GGTTCCTGTG GGCAGTGTAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCACTTT GGTCTCTTCT ATAGATAGTG CACATTCAGA TGTTGCTTCA 
GGTATGAGCG ATGGCAATGA GAATGTGCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGTCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAGTTA 
GAGACTCACA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCCGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCGTTT 
GTGGATCAAG TACGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCCG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA AGGAGAGTTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GCATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC TGGAAGACGG 
TTTATAGTGA GTCCTGTACC AGAAAGCCGA TTACGAGAAT CAAAAATTTT CACCAGTGAA 
ATATCAGATA CGGTTGCCGC CTCTACATCT CATGGCCCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTTA GACATGCCCA AGTTGCAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATATAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAATCCCA ACGACAGCAG CAGCCGTACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC ATTTCCACAT CAATAATTCA ATCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCACC TGCGTCTGAA TCACCAATTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CAACAGTTGT CTCAGTATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TATAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCAGTGC TGCTGCCCCA GGGGCTAAGT CTCCACCTTT GTTATCTGAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCACTCCA 
TTCCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCACTAGATA AAACATCTCA TAGCAGTACA 
ACTGGACTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAACC TGGTGGATTA CATCCTTTGG TCATCCCATC AGCTGTAGTT 
TCTACTCCTG TCCTTTCCCA AGCATCAGGA CCTATTTCTA CCCCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CTCAACCAGC CCCATACGCA CTGCCCTGAA 
ATAGATGCTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TCTAGAGGAA 
AAGTTGCGGT CTCTCTTTAG TGAACACAGC TCATCTGGCG CTCAACATGC CTCTGTTGCG 
CTGGAGACAT CACTAGTAGT AGAGACCAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CATTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCA 
GTCAGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG CCTGTGTCCG TGGTGGCTCC AGCGGCAGTT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCACAGAAGG TTCTGTCCCA 
GCAGCTAGTT CAGGAACTGG TGTTTTTAAG ACGGGGCGAT TTCAGGTTTC AGTTGCAATA 
GATGATTCCC AGAAAGAGGG TAAAACTAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CCTGGTGAAG 
CCAGACTCTA ATGGGGTAAC CATCCATGGC ATCTCTTCAG ATATGCCAGA TAGTGCCCAC 
AAAACTCCTG CCGCAGAAGC AAAGTCAGAA ACTGGGCAAC CTACCAAGGT TGGGCGTTTT 
CAGGTGACAA CTACAGCAAA CAAAGTAGGT CGTTTCTCTG TATCAAGAAC TGAAGACAGG 
CTCACTGAGG CAAAGAAAGA GGGACCGACA GCATCTCCTC CTTTTATGGA TTTGGAACAA 
CCTGTTCTTC CTGCTGCAAT ACCAAAGAAA GAAAAGCCTG AACTGTCAGA GCCTTCACAT 
CTGAATGGGC CATCTTCTGA TTTGGAGGCT GCCTTCCTAA GTAGGGATGT AGATGATGGT 
TCAGGTAGTC CACACTCCCC CCACCAACTG AGCTCAAAGA GCCTACCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAACTC ACTCAACTCC TCTTATATGA GTAGTGACAA TGAGTCAGAT 
ATTGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGACAAACA TCTTAAAGAA 
ATTCAAGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC TAAACTGGGC 
AAGGTTCCTC CTGCTGTCAT TATTCCCCCA GCGGCCCCCC TTTCAGGAAG AAGAAGGCGA 
CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGTATCAGTT CCTTGGGGAG TAAAAGCCCC 
CAGCTTTCAG GTAATATGTC TGGTCAGAGT GAAGCTTCAG TCTTACACCC CCAGCAGACC 
CTCCACCCTC CAGGCAACAT CCCAGAAACT GGGCAGAATC AGTTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTGTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG TCAAGGGACC AGCAGCACAA ACACTGTCGG GGGAACAGTG 
AATAGCCAAG CTGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACGTTC 
ACAGATGACC TGCACAAGTT GGTAGACAAT TGGGCCAGAG ATGCCATGAA TCTTTCAGGC 
AGGAGAGGAA GCAAAGGACA CATGAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTCTCT 
GCACCTGGTC AGCTGTGCAT CTCCATGACC TCAAATCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAT 
GGTTTTCCAC CTACCCCCTT TGGCACTCAG TGGAGTGGGA CAGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACC ACCTCCTTAC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011366127.1
CDS	1012..8082
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHPQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPVSQIPVSTPHVSAAQTGFSTLPITIAAGLNQPLLTLASSATAAAIPGGSTMAPSQLPALLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIAPSSSVASVCIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLLVPVGSVGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011367825.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011367825.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG TCTCTCAAAT TCCTGTATCA ACTCCTCATG TGTCCGCGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCCTTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCCGC AATCCCAGGG GGATCAACTA TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT CCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTCCCTCTTC CAGCGTGGCT TCTGTTTGCA TTCCTTCTAC AGTCTTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTTT GGTTCCTGTG GGCAGTGTAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCACTTT GGTCTCTTCT ATAGATAGTG CACATTCAGA TGTTGCTTCA 
GGTATGAGCG ATGGCAATGA GAATGTGCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGTCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAGTTA 
GAGACTCACA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCCGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCGTTT 
GTGGATCAAG TACGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCCG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA AGGAGAGTTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GCATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC TGGAAGACGG 
TTTATAGTGA GTCCTGTACC AGAAAGCCGA TTACGAGAAT CAAAAATTTT CACCAGTGAA 
ATATCAGATA CGGTTGCCGC CTCTACATCT CATGGCCCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTTA GACATGCCCA AGTTGCAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATATAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAATCCCA ACGACAGCAG CAGCCGTACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC ATTTCCACAT CAATAATTCA ATCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCACC TGCGTCTGAA TCACCAATTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CAACAGTTGT CTCAGTATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TATAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCAGTGC TGCTGCCCCA GGGGCTAAGT CTCCACCTTT GTTATCTGAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCACTCCA 
TTCCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCACTAGATA AAACATCTCA TAGCAGTACA 
ACTGGACTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAACC TGGTGGATTA CATCCTTTGG TCATCCCATC AGCTGTAGTT 
TCTACTCCTG TCCTTTCCCA AGCATCAGGA CCTATTTCTA CCCCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CTCAACCAGC CCCATACGCA CTGCCCTGAA 
ATAGATGCTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TCTAGAGGAA 
AAGTTGCGGT CTCTCTTTAG TGAACACAGC TCATCTGGCG CTCAACATGC CTCTGTTGCG 
CTGGAGACAT CACTAGTAGT AGAGACCAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CATTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCA 
GTCAGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG CCTGTGTCCG TGGTGGCTCC AGCGGCAGTT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCACAGAAGG TTCTGTCCCA 
GCAGCTAGTT CAGGAACTGG TGTTTTTAAG ACGGGGCGAT TTCAGGTTTC AGTTGCAATA 
GATGATTCCC AGAAAGAGGG TAAAACTAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CCTGGTGAAG 
CCAGACTCTA ATGGGGTAAC CATCCATGGC ATCTCTTCAG ATATGCCAGA TAGTGCCCAC 
AAAACTCCTG CCGCAGAAGC AAAGTCAGAA ACTGGGCAAC CTACCAAGGT TGGGCGTTTT 
CAGGTGACAA CTACAGCAAA CAAAGTAGGT CGTTTCTCTG TATCAAGAAC TGAAGACAGG 
CTCACTGAGG CAAAGAAAGA GGGACCGACA GCATCTCCTC CTTTTATGGA TTTGGAACAA 
CCTGTTCTTC CTGCTGCAAT ACCAAAGAAA GAAAAGCCTG AACTGTCAGA GCCTTCACAT 
CTGAATGGGC CATCTTCTGA TTTGGAGGCT GCCTTCCTAA GTAGGGATGT AGATGATGGT 
TCAGGTAGTC CACACTCCCC CCACCAACTG AGCTCAAAGA GCCTACCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAACTC ACTCAACTCC TCTTATATGA GTAGTGACAA TGAGTCAGAT 
ATTGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGACAAACA TCTTAAAGAA 
ATTCAAGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC TAAACTGGGC 
AAGGTTCCTC CTGCTGTCAT TATTCCCCCA GCGGCCCCCC TTTCAGGAAG AAGAAGGCGA 
CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGTATCAGTT CCTTGGGGAG TAAAAGCCCC 
CAGCTTTCAG GTAATATGTC TGGTCAGAGT GAAGCTTCAG TCTTACACCC CCAGCAGACC 
CTCCACCCTC CAGGCAACAT CCCAGAAACT GGGCAGAATC AGTTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTGTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG TCAAGGGACC AGCAGCACAA ACACTGTCGG GGGAACAGTG 
AATAGCCAAG CTGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACGTTC 
ACAGATGACC TGCACAAGTT GGTAGACAAT TGGGCCAGAG ATGCCATGAA TCTTTCAGGC 
AGGAGAGGAA GCAAAGGACA CATGAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTCTCT 
GCACCTGGTC AGCTGTGCAT CTCCATGACC TCAAATCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAT 
GGTTTTCCAC CTACCCCCTT TGGCACTCAG TGGAGTGGGA CAGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACC ACCTCCTTAC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011367826.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011366128.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

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CloneID	OPt52297
Clone ID Related Accession (Same CDS sequence)	XM_011367826.1
Accession Version	XM_011367826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG TCTCTCAAAT TCCTGTATCA ACTCCTCATG TGTCCGCGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCCTTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCCGC AATCCCAGGG GGATCAACTA TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT CCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GAGTACTCAG GGAGTCTCTC AGGTTGCTCC TCCAGAGCCA 
GTTTCGGTAG CACAGCCACA GCCCACCCAG CCTGCCACTT TGGTCTCTTC TATAGATAGT 
GCACATTCAG ATGTTGCTTC AGGTATGAGC GATGGCAATG AGAATGTGCC ATCATCTGGT 
GGAAGGCATG AAGGGAGAAC TGCAAAACGG CATTATCGAA AATCTGTAAG AAGTCGCTCT 
CGTCATGAAA AGACTTCACG CCCCAAATTG AGAATTTTGA ATGTTTCAAA TAAAGGAGAC 
CGGGTAGTAG AATGTCAGTT AGAGACTCAC AATCGGAAAA TGGTTACATT CAAATTTGAT 
TTAGATGGTG ACAATCCCGA GGAGATAGCA ACAATTATGG TGAACAATGA CTTTATTCTA 
GCAATAGAGA GAGAGTCGTT TGTGGATCAA GTACGGGAAA TTATTGAAAA AGCTGATGAA 
ATGCTCAGTG AGGATGTCAG TGTGGAACCC GAGGGTGACC AGGGATTGGA GAGTCTACAG 
GGAAAGGATG ACTATGGCTT TTCAGGTTCT CAGAAATTGG AAGGAGAGTT CAAACAACCA 
CTTCCTGCAT CTTCCATGCC ACAGCAAATA GGCATTCCTA CCAGTTCTTT AACTCAAGTT 
GTTCATTCTG CTGGAAGACG GTTTATAGTG AGTCCTGTAC CAGAAAGCCG ATTACGAGAA 
TCAAAAATTT TCACCAGTGA AATATCAGAT ACGGTTGCCG CCTCTACATC TCATGGCCCT 
GGGGTGAACT TGTCCCACTC TGCTTCTTCC CTTAGTCTAC AGCAGGCCTT TTCTGAACTT 
AGACATGCCC AAGTTGCAGA GGGACCCAAT ACAGCACCTC CCAACTTCAG TCATATAGGA 
CCAACATTTC CAGTAGTACC TCCTTCCATG AGTAGCATTG CTGGAATCCC AACGACAGCA 
GCAGCCGTAC CTTCAATATC AGTCCCTGTA ACTAGCTGCC CTCTTAATGA CATTTCCACA 
TCAATAATTC AATCTGAGAT TACAGTGCCC ACTGAAAAAG GAGTTGCTGG AGTTGCCACC 
TGCACAGGTG TAATAACTTC AAGTGGTTTC CCAGTACCAC CTGCGTCTGA ATCACCAATT 
CTTTCCAGTG TGGTTTCAAG TATCACAATA CCAACAGTTG TCTCAGTATC AACAACATCC 
CAGTCAGTTC AGGCTCCCAC ATCTGGGAGC GTTGTTTCTA GTATAGGCAC TTTCCCTTCT 
ATACCAGTTT CAGCAACTTC AGCCTCTGCA GTGGGCAGTG CTGCTGCCCC AGGGGCTAAG 
TCTCCACCTT TGTTATCTGA GCAGTCAGCA GGCATTACTA CAGGGATAGC CACATTAACA 
TCAGTTTCTA CCACCACTCC ATTCCCAAGC ACAGCTTCAC AGCTATCTCT TCAGCTTAGC 
AGCAGCACCT CTGCTCCTAC TCTAGCTGAA ACAGTGGTGG TTAGTGCACA CTCACTAGAT 
AAAACATCTC ATAGCAGTAC AACTGGACTG GCTTTGTCCC ACTCTGCATC ATCTTCCACT 
TCTTCCCCTG GAGCAGGAGT ATCTAGTTCT ATTTCTCAAC CTGGTGGATT ACATCCTTTG 
GTCATCCCAT CAGCTGTAGT TTCTACTCCT GTCCTTTCCC AAGCATCAGG ACCTATTTCT 
ACCCCTTTAT TACCCCAAGG ACCTAGCATC CCACCATTGG TGCAACCTGT TGCCAATGTG 
CCTGCTGTAC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTGCT TCTCAACCAG 
CCCCATACGC ACTGCCCTGA AATAGATGCT GATACACAAC CCAAAGCTCC TGGGATTGAT 
GACATAAAGA CTCTAGAGGA AAAGTTGCGG TCTCTCTTTA GTGAACACAG CTCATCTGGC 
GCTCAACATG CCTCTGTTGC GCTGGAGACA TCACTAGTAG TAGAGACCAG TGTCACACCA 
GGCATCCCAA CCACTGCTGT TGCACCCAGC AAACTCATGA CTTCAACTAC AAGTACTTGC 
TTACCACCAA CCAGTTTGCC ATTAGGAACA GCTGGTTTGT CCATTACACC ACTGGTCACA 
ACTGGGCAAG TTTCTACCCC AGTCAGCACT ACATCAGGAG TGAAACCTGG AACTGCTCCA 
TCAAAGCTAC CTTTAACTAA GGCTCCAGTG CTGCCAGTGG GTACTGAACT TCCAGCTGGT 
ACTCCATCCA GTGAGCAGCT GCCACCTATT CCAGGACCTT CACTAACTCA GCCTGTGTCC 
GTGGTGGCTC CAGCGGCAGT TACAGAAGCA GGAGCACAGC CTCAGAAGGA TGTTTCTCAA 
GTCACAGAAG GTTCTGTCCC AGCAGCTAGT TCAGGAACTG GTGTTTTTAA GACGGGGCGA 
TTTCAGGTTT CAGTTGCAAT AGATGATTCC CAGAAAGAGG GTAAAACTAA GTCAGAAGAT 
GCAAAGTCTG TTCATTTTGA ATCCAGCACT TCAGAGTCCT CAGTGCTATC AAGTAGTAGT 
CCAGAGAGTA CCCTGGTGAA GCCAGACTCT AATGGGGTAA CCATCCATGG CATCTCTTCA 
GATATGCCAG ATAGTGCCCA CAAAACTCCT GCCGCAGAAG CAAAGTCAGA AACTGGGCAA 
CCTACCAAGG TTGGGCGTTT TCAGGTGACA ACTACAGCAA ACAAAGTAGG TCGTTTCTCT 
GTATCAAGAA CTGAAGACAG GCTCACTGAG GCAAAGAAAG AGGGACCGAC AGCATCTCCT 
CCTTTTATGG ATTTGGAACA ACCTGTTCTT CCTGCTGCAA TACCAAAGAA AGAAAAGCCT 
GAACTGTCAG AGCCTTCACA TCTGAATGGG CCATCTTCTG ATTTGGAGGC TGCCTTCCTA 
AGTAGGGATG TAGATGATGG TTCAGGTAGT CCACACTCCC CCCACCAACT GAGCTCAAAG 
AGCCTACCTA TCCAGAATCT AAGTCAAAGC CTTAGTAACT CACTCAACTC CTCTTATATG 
AGTAGTGACA ATGAGTCAGA TATTGAAGAT GAAGACTTAA AGTTAGAGCT GCGACGACTA 
CGAGACAAAC ATCTTAAAGA AATTCAAGAC CTGCAGAGTC GCCAGAAGCA TGAAATTGAA 
TCTTTGTATA CTAAACTGGG CAAGGTTCCT CCTGCTGTCA TTATTCCCCC AGCGGCCCCC 
CTTTCAGGAA GAAGAAGGCG ACCCACTAAA AGCAAAGGCA GCAAATCTAG TCGTATCAGT 
TCCTTGGGGA GTAAAAGCCC CCAGCTTTCA GGTAATATGT CTGGTCAGAG TGAAGCTTCA 
GTCTTACACC CCCAGCAGAC CCTCCACCCT CCAGGCAACA TCCCAGAAAC TGGGCAGAAT 
CAGTTGTTAC AGCCCCTTAA GCCATCTCCC TCCAGTGACA ACCTGTATTC AGCCTTCACC 
AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GTCAAGGGAC CAGCAGCACA 
AACACTGTCG GGGGAACAGT GAATAGCCAA GCTGCCCAAG CTCAGCCTCC TGCCATGACG 
TCCAGCAGGA AGGGCACGTT CACAGATGAC CTGCACAAGT TGGTAGACAA TTGGGCCAGA 
GATGCCATGA ATCTTTCAGG CAGGAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT 
GGAATGGCAA GGAAGTTCTC TGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG 
GGTGGCTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA TGGTTTTCCA CCTACCCCCT TTGGCACTCA GTGGAGTGGG 
ACAGGTGGCC CAGCACCACA GCCACTTGGC CAGTTCCAAC CTGTGGGAAC CACCTCCTTA 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG 
CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011366128.1
CDS	1011..7802
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHPQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPVSQIPVSTPHVSAAQTGFSTLPITIAAGLNQPLLTLASSATAAAIPGGSTMAPSQLPALLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQSTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011367826.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011367826.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG TCTCTCAAAT TCCTGTATCA ACTCCTCATG TGTCCGCGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCCTTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCCGC AATCCCAGGG GGATCAACTA TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT CCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GAGTACTCAG GGAGTCTCTC AGGTTGCTCC TCCAGAGCCA 
GTTTCGGTAG CACAGCCACA GCCCACCCAG CCTGCCACTT TGGTCTCTTC TATAGATAGT 
GCACATTCAG ATGTTGCTTC AGGTATGAGC GATGGCAATG AGAATGTGCC ATCATCTGGT 
GGAAGGCATG AAGGGAGAAC TGCAAAACGG CATTATCGAA AATCTGTAAG AAGTCGCTCT 
CGTCATGAAA AGACTTCACG CCCCAAATTG AGAATTTTGA ATGTTTCAAA TAAAGGAGAC 
CGGGTAGTAG AATGTCAGTT AGAGACTCAC AATCGGAAAA TGGTTACATT CAAATTTGAT 
TTAGATGGTG ACAATCCCGA GGAGATAGCA ACAATTATGG TGAACAATGA CTTTATTCTA 
GCAATAGAGA GAGAGTCGTT TGTGGATCAA GTACGGGAAA TTATTGAAAA AGCTGATGAA 
ATGCTCAGTG AGGATGTCAG TGTGGAACCC GAGGGTGACC AGGGATTGGA GAGTCTACAG 
GGAAAGGATG ACTATGGCTT TTCAGGTTCT CAGAAATTGG AAGGAGAGTT CAAACAACCA 
CTTCCTGCAT CTTCCATGCC ACAGCAAATA GGCATTCCTA CCAGTTCTTT AACTCAAGTT 
GTTCATTCTG CTGGAAGACG GTTTATAGTG AGTCCTGTAC CAGAAAGCCG ATTACGAGAA 
TCAAAAATTT TCACCAGTGA AATATCAGAT ACGGTTGCCG CCTCTACATC TCATGGCCCT 
GGGGTGAACT TGTCCCACTC TGCTTCTTCC CTTAGTCTAC AGCAGGCCTT TTCTGAACTT 
AGACATGCCC AAGTTGCAGA GGGACCCAAT ACAGCACCTC CCAACTTCAG TCATATAGGA 
CCAACATTTC CAGTAGTACC TCCTTCCATG AGTAGCATTG CTGGAATCCC AACGACAGCA 
GCAGCCGTAC CTTCAATATC AGTCCCTGTA ACTAGCTGCC CTCTTAATGA CATTTCCACA 
TCAATAATTC AATCTGAGAT TACAGTGCCC ACTGAAAAAG GAGTTGCTGG AGTTGCCACC 
TGCACAGGTG TAATAACTTC AAGTGGTTTC CCAGTACCAC CTGCGTCTGA ATCACCAATT 
CTTTCCAGTG TGGTTTCAAG TATCACAATA CCAACAGTTG TCTCAGTATC AACAACATCC 
CAGTCAGTTC AGGCTCCCAC ATCTGGGAGC GTTGTTTCTA GTATAGGCAC TTTCCCTTCT 
ATACCAGTTT CAGCAACTTC AGCCTCTGCA GTGGGCAGTG CTGCTGCCCC AGGGGCTAAG 
TCTCCACCTT TGTTATCTGA GCAGTCAGCA GGCATTACTA CAGGGATAGC CACATTAACA 
TCAGTTTCTA CCACCACTCC ATTCCCAAGC ACAGCTTCAC AGCTATCTCT TCAGCTTAGC 
AGCAGCACCT CTGCTCCTAC TCTAGCTGAA ACAGTGGTGG TTAGTGCACA CTCACTAGAT 
AAAACATCTC ATAGCAGTAC AACTGGACTG GCTTTGTCCC ACTCTGCATC ATCTTCCACT 
TCTTCCCCTG GAGCAGGAGT ATCTAGTTCT ATTTCTCAAC CTGGTGGATT ACATCCTTTG 
GTCATCCCAT CAGCTGTAGT TTCTACTCCT GTCCTTTCCC AAGCATCAGG ACCTATTTCT 
ACCCCTTTAT TACCCCAAGG ACCTAGCATC CCACCATTGG TGCAACCTGT TGCCAATGTG 
CCTGCTGTAC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTGCT TCTCAACCAG 
CCCCATACGC ACTGCCCTGA AATAGATGCT GATACACAAC CCAAAGCTCC TGGGATTGAT 
GACATAAAGA CTCTAGAGGA AAAGTTGCGG TCTCTCTTTA GTGAACACAG CTCATCTGGC 
GCTCAACATG CCTCTGTTGC GCTGGAGACA TCACTAGTAG TAGAGACCAG TGTCACACCA 
GGCATCCCAA CCACTGCTGT TGCACCCAGC AAACTCATGA CTTCAACTAC AAGTACTTGC 
TTACCACCAA CCAGTTTGCC ATTAGGAACA GCTGGTTTGT CCATTACACC ACTGGTCACA 
ACTGGGCAAG TTTCTACCCC AGTCAGCACT ACATCAGGAG TGAAACCTGG AACTGCTCCA 
TCAAAGCTAC CTTTAACTAA GGCTCCAGTG CTGCCAGTGG GTACTGAACT TCCAGCTGGT 
ACTCCATCCA GTGAGCAGCT GCCACCTATT CCAGGACCTT CACTAACTCA GCCTGTGTCC 
GTGGTGGCTC CAGCGGCAGT TACAGAAGCA GGAGCACAGC CTCAGAAGGA TGTTTCTCAA 
GTCACAGAAG GTTCTGTCCC AGCAGCTAGT TCAGGAACTG GTGTTTTTAA GACGGGGCGA 
TTTCAGGTTT CAGTTGCAAT AGATGATTCC CAGAAAGAGG GTAAAACTAA GTCAGAAGAT 
GCAAAGTCTG TTCATTTTGA ATCCAGCACT TCAGAGTCCT CAGTGCTATC AAGTAGTAGT 
CCAGAGAGTA CCCTGGTGAA GCCAGACTCT AATGGGGTAA CCATCCATGG CATCTCTTCA 
GATATGCCAG ATAGTGCCCA CAAAACTCCT GCCGCAGAAG CAAAGTCAGA AACTGGGCAA 
CCTACCAAGG TTGGGCGTTT TCAGGTGACA ACTACAGCAA ACAAAGTAGG TCGTTTCTCT 
GTATCAAGAA CTGAAGACAG GCTCACTGAG GCAAAGAAAG AGGGACCGAC AGCATCTCCT 
CCTTTTATGG ATTTGGAACA ACCTGTTCTT CCTGCTGCAA TACCAAAGAA AGAAAAGCCT 
GAACTGTCAG AGCCTTCACA TCTGAATGGG CCATCTTCTG ATTTGGAGGC TGCCTTCCTA 
AGTAGGGATG TAGATGATGG TTCAGGTAGT CCACACTCCC CCCACCAACT GAGCTCAAAG 
AGCCTACCTA TCCAGAATCT AAGTCAAAGC CTTAGTAACT CACTCAACTC CTCTTATATG 
AGTAGTGACA ATGAGTCAGA TATTGAAGAT GAAGACTTAA AGTTAGAGCT GCGACGACTA 
CGAGACAAAC ATCTTAAAGA AATTCAAGAC CTGCAGAGTC GCCAGAAGCA TGAAATTGAA 
TCTTTGTATA CTAAACTGGG CAAGGTTCCT CCTGCTGTCA TTATTCCCCC AGCGGCCCCC 
CTTTCAGGAA GAAGAAGGCG ACCCACTAAA AGCAAAGGCA GCAAATCTAG TCGTATCAGT 
TCCTTGGGGA GTAAAAGCCC CCAGCTTTCA GGTAATATGT CTGGTCAGAG TGAAGCTTCA 
GTCTTACACC CCCAGCAGAC CCTCCACCCT CCAGGCAACA TCCCAGAAAC TGGGCAGAAT 
CAGTTGTTAC AGCCCCTTAA GCCATCTCCC TCCAGTGACA ACCTGTATTC AGCCTTCACC 
AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GTCAAGGGAC CAGCAGCACA 
AACACTGTCG GGGGAACAGT GAATAGCCAA GCTGCCCAAG CTCAGCCTCC TGCCATGACG 
TCCAGCAGGA AGGGCACGTT CACAGATGAC CTGCACAAGT TGGTAGACAA TTGGGCCAGA 
GATGCCATGA ATCTTTCAGG CAGGAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT 
GGAATGGCAA GGAAGTTCTC TGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG 
GGTGGCTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA TGGTTTTCCA CCTACCCCCT TTGGCACTCA GTGGAGTGGG 
ACAGGTGGCC CAGCACCACA GCCACTTGGC CAGTTCCAAC CTGTGGGAAC CACCTCCTTA 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG 
CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011367827.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011366129.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

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CloneID	OPt52298
Clone ID Related Accession (Same CDS sequence)	XM_011367827.1 , XM_011367827.2
Accession Version	XM_011367827.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCATGGAAT ACAGCAGACA GCCCCTCCTC AACAGACAGT ACAGTATTCA 
CTTCCACAGA CATCAACCTC CAGTGAGGCC ACTACTGCAC AGCCAGTGAG TCAGCCTCAG 
GCTCCACAGG TCTTGCCTCA AGCATCAGCT GGAAAACAGA GTACTCAGGG AGTCTCTCAG 
GTTGCTCCTC CAGAGCCAGT TTCGGTAGCA CAGCCACAGC CCACCCAGCC TGCCACTTTG 
GTCTCTTCTA TAGATAGTGC ACATTCAGAT GTTGCTTCAG GTATGAGCGA TGGCAATGAG 
AATGTGCCAT CATCTGGTGG AAGGCATGAA GGGAGAACTG CAAAACGGCA TTATCGAAAA 
TCTGTAAGAA GTCGCTCTCG TCATGAAAAG ACTTCACGCC CCAAATTGAG AATTTTGAAT 
GTTTCAAATA AAGGAGACCG GGTAGTAGAA TGTCAGTTAG AGACTCACAA TCGGAAAATG 
GTTACATTCA AATTTGATTT AGATGGTGAC AATCCCGAGG AGATAGCAAC AATTATGGTG 
AACAATGACT TTATTCTAGC AATAGAGAGA GAGTCGTTTG TGGATCAAGT ACGGGAAATT 
ATTGAAAAAG CTGATGAAAT GCTCAGTGAG GATGTCAGTG TGGAACCCGA GGGTGACCAG 
GGATTGGAGA GTCTACAGGG AAAGGATGAC TATGGCTTTT CAGGTTCTCA GAAATTGGAA 
GGAGAGTTCA AACAACCACT TCCTGCATCT TCCATGCCAC AGCAAATAGG CATTCCTACC 
AGTTCTTTAA CTCAAGTTGT TCATTCTGCT GGAAGACGGT TTATAGTGAG TCCTGTACCA 
GAAAGCCGAT TACGAGAATC AAAAATTTTC ACCAGTGAAA TATCAGATAC GGTTGCCGCC 
TCTACATCTC ATGGCCCTGG GGTGAACTTG TCCCACTCTG CTTCTTCCCT TAGTCTACAG 
CAGGCCTTTT CTGAACTTAG ACATGCCCAA GTTGCAGAGG GACCCAATAC AGCACCTCCC 
AACTTCAGTC ATATAGGACC AACATTTCCA GTAGTACCTC CTTCCATGAG TAGCATTGCT 
GGAATCCCAA CGACAGCAGC AGCCGTACCT TCAATATCAG TCCCTGTAAC TAGCTGCCCT 
CTTAATGACA TTTCCACATC AATAATTCAA TCTGAGATTA CAGTGCCCAC TGAAAAAGGA 
GTTGCTGGAG TTGCCACCTG CACAGGTGTA ATAACTTCAA GTGGTTTCCC AGTACCACCT 
GCGTCTGAAT CACCAATTCT TTCCAGTGTG GTTTCAAGTA TCACAATACC AACAGTTGTC 
TCAGTATCAA CAACATCCCA GTCAGTTCAG GCTCCCACAT CTGGGAGCGT TGTTTCTAGT 
ATAGGCACTT TCCCTTCTAT ACCAGTTTCA GCAACTTCAG CCTCTGCAGT GGGCAGTGCT 
GCTGCCCCAG GGGCTAAGTC TCCACCTTTG TTATCTGAGC AGTCAGCAGG CATTACTACA 
GGGATAGCCA CATTAACATC AGTTTCTACC ACCACTCCAT TCCCAAGCAC AGCTTCACAG 
CTATCTCTTC AGCTTAGCAG CAGCACCTCT GCTCCTACTC TAGCTGAAAC AGTGGTGGTT 
AGTGCACACT CACTAGATAA AACATCTCAT AGCAGTACAA CTGGACTGGC TTTGTCCCAC 
TCTGCATCAT CTTCCACTTC TTCCCCTGGA GCAGGAGTAT CTAGTTCTAT TTCTCAACCT 
GGTGGATTAC ATCCTTTGGT CATCCCATCA GCTGTAGTTT CTACTCCTGT CCTTTCCCAA 
GCATCAGGAC CTATTTCTAC CCCTTTATTA CCCCAAGGAC CTAGCATCCC ACCATTGGTG 
CAACCTGTTG CCAATGTGCC TGCTGTACAG CAGACACTAA TTCATAGTCA GCCTCAACCA 
GCTTTGCTTC TCAACCAGCC CCATACGCAC TGCCCTGAAA TAGATGCTGA TACACAACCC 
AAAGCTCCTG GGATTGATGA CATAAAGACT CTAGAGGAAA AGTTGCGGTC TCTCTTTAGT 
GAACACAGCT CATCTGGCGC TCAACATGCC TCTGTTGCGC TGGAGACATC ACTAGTAGTA 
GAGACCAGTG TCACACCAGG CATCCCAACC ACTGCTGTTG CACCCAGCAA ACTCATGACT 
TCAACTACAA GTACTTGCTT ACCACCAACC AGTTTGCCAT TAGGAACAGC TGGTTTGTCC 
ATTACACCAC TGGTCACAAC TGGGCAAGTT TCTACCCCAG TCAGCACTAC ATCAGGAGTG 
AAACCTGGAA CTGCTCCATC AAAGCTACCT TTAACTAAGG CTCCAGTGCT GCCAGTGGGT 
ACTGAACTTC CAGCTGGTAC TCCATCCAGT GAGCAGCTGC CACCTATTCC AGGACCTTCA 
CTAACTCAGT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTAAGAAG AGCACTTAGT 
CCAGAGGCTA TTGCGGTGAC ATCCGCAGTT GGTCCTGTGT CCGTGGTGGC TCCAGCGGCA 
GTTACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AAGTCACAGA AGGTTCTGTC 
CCAGCAGCTA GTTCAGGAAC TGGTGTTTTT AAGACGGGGC GATTTCAGGT TTCAGTTGCA 
ATAGATGATT CCCAGAAAGA GGGTAAAACT AAGTCAGAAG ATGCAAAGTC TGTTCATTTT 
GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAGAG TACCCTGGTG 
AAGCCAGACT CTAATGGGGT AACCATCCAT GGCATCTCTT CAGATATGCC AGATAGTGCC 
CACAAAACTC CTGCCGCAGA AGCAAAGTCA GAAACTGGGC AACCTACCAA GGTTGGGCGT 
TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTATCAAG AACTGAAGAC 
AGGCTCACTG AGGCAAAGAA AGAGGGACCG ACAGCATCTC CTCCTTTTAT GGATTTGGAA 
CAACCTGTTC TTCCTGCTGC AATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA 
CATCTGAATG GGCCATCTTC TGATTTGGAG GCTGCCTTCC TAAGTAGGGA TGTAGATGAT 
GGTTCAGGTA GTCCACACTC CCCCCACCAA CTGAGCTCAA AGAGCCTACC TATCCAGAAT 
CTAAGTCAAA GCCTTAGTAA CTCACTCAAC TCCTCTTATA TGAGTAGTGA CAATGAGTCA 
GATATTGAAG ATGAAGACTT AAAGTTAGAG CTGCGACGAC TACGAGACAA ACATCTTAAA 
GAAATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG 
GGCAAGGTTC CTCCTGCTGT CATTATTCCC CCAGCGGCCC CCCTTTCAGG AAGAAGAAGG 
CGACCCACTA AAAGCAAAGG CAGCAAATCT AGTCGTATCA GTTCCTTGGG GAGTAAAAGC 
CCCCAGCTTT CAGGTAATAT GTCTGGTCAG AGTGAAGCTT CAGTCTTACA CCCCCAGCAG 
ACCCTCCACC CTCCAGGCAA CATCCCAGAA ACTGGGCAGA ATCAGTTGTT ACAGCCCCTT 
AAGCCATCTC CCTCCAGTGA CAACCTGTAT TCAGCCTTCA CCAGTGATGG TGCCATTTCA 
GTACCAAGCC TTTCTGCTCC AGGTCAAGGG ACCAGCAGCA CAAACACTGT CGGGGGAACA 
GTGAATAGCC AAGCTGCCCA AGCTCAGCCT CCTGCCATGA CGTCCAGCAG GAAGGGCACG 
TTCACAGATG ACCTGCACAA GTTGGTAGAC AATTGGGCCA GAGATGCCAT GAATCTTTCA 
GGCAGGAGAG GAAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC 
TCTGCACCTG GTCAGCTGTG CATCTCCATG ACCTCAAATC TGGGTGGCTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TATGGTTTTC CACCTACCCC CTTTGGCACT CAGTGGAGTG GGACAGGTGG CCCAGCACCA 
CAGCCACTTG GCCAGTTCCA ACCTGTGGGA ACCACCTCCT TACAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011366129.1
CDS	1011..7424
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHPQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQSTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPEAIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011367827.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011367827.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCATGGAAT ACAGCAGACA GCCCCTCCTC AACAGACAGT ACAGTATTCA 
CTTCCACAGA CATCAACCTC CAGTGAGGCC ACTACTGCAC AGCCAGTGAG TCAGCCTCAG 
GCTCCACAGG TCTTGCCTCA AGCATCAGCT GGAAAACAGA GTACTCAGGG AGTCTCTCAG 
GTTGCTCCTC CAGAGCCAGT TTCGGTAGCA CAGCCACAGC CCACCCAGCC TGCCACTTTG 
GTCTCTTCTA TAGATAGTGC ACATTCAGAT GTTGCTTCAG GTATGAGCGA TGGCAATGAG 
AATGTGCCAT CATCTGGTGG AAGGCATGAA GGGAGAACTG CAAAACGGCA TTATCGAAAA 
TCTGTAAGAA GTCGCTCTCG TCATGAAAAG ACTTCACGCC CCAAATTGAG AATTTTGAAT 
GTTTCAAATA AAGGAGACCG GGTAGTAGAA TGTCAGTTAG AGACTCACAA TCGGAAAATG 
GTTACATTCA AATTTGATTT AGATGGTGAC AATCCCGAGG AGATAGCAAC AATTATGGTG 
AACAATGACT TTATTCTAGC AATAGAGAGA GAGTCGTTTG TGGATCAAGT ACGGGAAATT 
ATTGAAAAAG CTGATGAAAT GCTCAGTGAG GATGTCAGTG TGGAACCCGA GGGTGACCAG 
GGATTGGAGA GTCTACAGGG AAAGGATGAC TATGGCTTTT CAGGTTCTCA GAAATTGGAA 
GGAGAGTTCA AACAACCACT TCCTGCATCT TCCATGCCAC AGCAAATAGG CATTCCTACC 
AGTTCTTTAA CTCAAGTTGT TCATTCTGCT GGAAGACGGT TTATAGTGAG TCCTGTACCA 
GAAAGCCGAT TACGAGAATC AAAAATTTTC ACCAGTGAAA TATCAGATAC GGTTGCCGCC 
TCTACATCTC ATGGCCCTGG GGTGAACTTG TCCCACTCTG CTTCTTCCCT TAGTCTACAG 
CAGGCCTTTT CTGAACTTAG ACATGCCCAA GTTGCAGAGG GACCCAATAC AGCACCTCCC 
AACTTCAGTC ATATAGGACC AACATTTCCA GTAGTACCTC CTTCCATGAG TAGCATTGCT 
GGAATCCCAA CGACAGCAGC AGCCGTACCT TCAATATCAG TCCCTGTAAC TAGCTGCCCT 
CTTAATGACA TTTCCACATC AATAATTCAA TCTGAGATTA CAGTGCCCAC TGAAAAAGGA 
GTTGCTGGAG TTGCCACCTG CACAGGTGTA ATAACTTCAA GTGGTTTCCC AGTACCACCT 
GCGTCTGAAT CACCAATTCT TTCCAGTGTG GTTTCAAGTA TCACAATACC AACAGTTGTC 
TCAGTATCAA CAACATCCCA GTCAGTTCAG GCTCCCACAT CTGGGAGCGT TGTTTCTAGT 
ATAGGCACTT TCCCTTCTAT ACCAGTTTCA GCAACTTCAG CCTCTGCAGT GGGCAGTGCT 
GCTGCCCCAG GGGCTAAGTC TCCACCTTTG TTATCTGAGC AGTCAGCAGG CATTACTACA 
GGGATAGCCA CATTAACATC AGTTTCTACC ACCACTCCAT TCCCAAGCAC AGCTTCACAG 
CTATCTCTTC AGCTTAGCAG CAGCACCTCT GCTCCTACTC TAGCTGAAAC AGTGGTGGTT 
AGTGCACACT CACTAGATAA AACATCTCAT AGCAGTACAA CTGGACTGGC TTTGTCCCAC 
TCTGCATCAT CTTCCACTTC TTCCCCTGGA GCAGGAGTAT CTAGTTCTAT TTCTCAACCT 
GGTGGATTAC ATCCTTTGGT CATCCCATCA GCTGTAGTTT CTACTCCTGT CCTTTCCCAA 
GCATCAGGAC CTATTTCTAC CCCTTTATTA CCCCAAGGAC CTAGCATCCC ACCATTGGTG 
CAACCTGTTG CCAATGTGCC TGCTGTACAG CAGACACTAA TTCATAGTCA GCCTCAACCA 
GCTTTGCTTC TCAACCAGCC CCATACGCAC TGCCCTGAAA TAGATGCTGA TACACAACCC 
AAAGCTCCTG GGATTGATGA CATAAAGACT CTAGAGGAAA AGTTGCGGTC TCTCTTTAGT 
GAACACAGCT CATCTGGCGC TCAACATGCC TCTGTTGCGC TGGAGACATC ACTAGTAGTA 
GAGACCAGTG TCACACCAGG CATCCCAACC ACTGCTGTTG CACCCAGCAA ACTCATGACT 
TCAACTACAA GTACTTGCTT ACCACCAACC AGTTTGCCAT TAGGAACAGC TGGTTTGTCC 
ATTACACCAC TGGTCACAAC TGGGCAAGTT TCTACCCCAG TCAGCACTAC ATCAGGAGTG 
AAACCTGGAA CTGCTCCATC AAAGCTACCT TTAACTAAGG CTCCAGTGCT GCCAGTGGGT 
ACTGAACTTC CAGCTGGTAC TCCATCCAGT GAGCAGCTGC CACCTATTCC AGGACCTTCA 
CTAACTCAGT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTAAGAAG AGCACTTAGT 
CCAGAGGCTA TTGCGGTGAC ATCCGCAGTT GGTCCTGTGT CCGTGGTGGC TCCAGCGGCA 
GTTACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AAGTCACAGA AGGTTCTGTC 
CCAGCAGCTA GTTCAGGAAC TGGTGTTTTT AAGACGGGGC GATTTCAGGT TTCAGTTGCA 
ATAGATGATT CCCAGAAAGA GGGTAAAACT AAGTCAGAAG ATGCAAAGTC TGTTCATTTT 
GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAGAG TACCCTGGTG 
AAGCCAGACT CTAATGGGGT AACCATCCAT GGCATCTCTT CAGATATGCC AGATAGTGCC 
CACAAAACTC CTGCCGCAGA AGCAAAGTCA GAAACTGGGC AACCTACCAA GGTTGGGCGT 
TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTATCAAG AACTGAAGAC 
AGGCTCACTG AGGCAAAGAA AGAGGGACCG ACAGCATCTC CTCCTTTTAT GGATTTGGAA 
CAACCTGTTC TTCCTGCTGC AATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA 
CATCTGAATG GGCCATCTTC TGATTTGGAG GCTGCCTTCC TAAGTAGGGA TGTAGATGAT 
GGTTCAGGTA GTCCACACTC CCCCCACCAA CTGAGCTCAA AGAGCCTACC TATCCAGAAT 
CTAAGTCAAA GCCTTAGTAA CTCACTCAAC TCCTCTTATA TGAGTAGTGA CAATGAGTCA 
GATATTGAAG ATGAAGACTT AAAGTTAGAG CTGCGACGAC TACGAGACAA ACATCTTAAA 
GAAATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG 
GGCAAGGTTC CTCCTGCTGT CATTATTCCC CCAGCGGCCC CCCTTTCAGG AAGAAGAAGG 
CGACCCACTA AAAGCAAAGG CAGCAAATCT AGTCGTATCA GTTCCTTGGG GAGTAAAAGC 
CCCCAGCTTT CAGGTAATAT GTCTGGTCAG AGTGAAGCTT CAGTCTTACA CCCCCAGCAG 
ACCCTCCACC CTCCAGGCAA CATCCCAGAA ACTGGGCAGA ATCAGTTGTT ACAGCCCCTT 
AAGCCATCTC CCTCCAGTGA CAACCTGTAT TCAGCCTTCA CCAGTGATGG TGCCATTTCA 
GTACCAAGCC TTTCTGCTCC AGGTCAAGGG ACCAGCAGCA CAAACACTGT CGGGGGAACA 
GTGAATAGCC AAGCTGCCCA AGCTCAGCCT CCTGCCATGA CGTCCAGCAG GAAGGGCACG 
TTCACAGATG ACCTGCACAA GTTGGTAGAC AATTGGGCCA GAGATGCCAT GAATCTTTCA 
GGCAGGAGAG GAAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC 
TCTGCACCTG GTCAGCTGTG CATCTCCATG ACCTCAAATC TGGGTGGCTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TATGGTTTTC CACCTACCCC CTTTGGCACT CAGTGGAGTG GGACAGGTGG CCCAGCACCA 
CAGCCACTTG GCCAGTTCCA ACCTGTGGGA ACCACCTCCT TACAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011367827.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011366129.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

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CloneID	OPt52298
Clone ID Related Accession (Same CDS sequence)	XM_011367827.1 , XM_011367827.2
Accession Version	XM_011367827.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011367827.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCATGGAAT ACAGCAGACA GCCCCTCCTC AACAGACAGT ACAGTATTCA 
CTTCCACAGA CATCAACCTC CAGTGAGGCC ACTACTGCAC AGCCAGTGAG TCAGCCTCAG 
GCTCCACAGG TCTTGCCTCA AGCATCAGCT GGAAAACAGA GTACTCAGGG AGTCTCTCAG 
GTTGCTCCTC CAGAGCCAGT TTCGGTAGCA CAGCCACAGC CCACCCAGCC TGCCACTTTG 
GTCTCTTCTA TAGATAGTGC ACATTCAGAT GTTGCTTCAG GTATGAGCGA TGGCAATGAG 
AATGTGCCAT CATCTGGTGG AAGGCATGAA GGGAGAACTG CAAAACGGCA TTATCGAAAA 
TCTGTAAGAA GTCGCTCTCG TCATGAAAAG ACTTCACGCC CCAAATTGAG AATTTTGAAT 
GTTTCAAATA AAGGAGACCG GGTAGTAGAA TGTCAGTTAG AGACTCACAA TCGGAAAATG 
GTTACATTCA AATTTGATTT AGATGGTGAC AATCCCGAGG AGATAGCAAC AATTATGGTG 
AACAATGACT TTATTCTAGC AATAGAGAGA GAGTCGTTTG TGGATCAAGT ACGGGAAATT 
ATTGAAAAAG CTGATGAAAT GCTCAGTGAG GATGTCAGTG TGGAACCCGA GGGTGACCAG 
GGATTGGAGA GTCTACAGGG AAAGGATGAC TATGGCTTTT CAGGTTCTCA GAAATTGGAA 
GGAGAGTTCA AACAACCACT TCCTGCATCT TCCATGCCAC AGCAAATAGG CATTCCTACC 
AGTTCTTTAA CTCAAGTTGT TCATTCTGCT GGAAGACGGT TTATAGTGAG TCCTGTACCA 
GAAAGCCGAT TACGAGAATC AAAAATTTTC ACCAGTGAAA TATCAGATAC GGTTGCCGCC 
TCTACATCTC ATGGCCCTGG GGTGAACTTG TCCCACTCTG CTTCTTCCCT TAGTCTACAG 
CAGGCCTTTT CTGAACTTAG ACATGCCCAA GTTGCAGAGG GACCCAATAC AGCACCTCCC 
AACTTCAGTC ATATAGGACC AACATTTCCA GTAGTACCTC CTTCCATGAG TAGCATTGCT 
GGAATCCCAA CGACAGCAGC AGCCGTACCT TCAATATCAG TCCCTGTAAC TAGCTGCCCT 
CTTAATGACA TTTCCACATC AATAATTCAA TCTGAGATTA CAGTGCCCAC TGAAAAAGGA 
GTTGCTGGAG TTGCCACCTG CACAGGTGTA ATAACTTCAA GTGGTTTCCC AGTACCACCT 
GCGTCTGAAT CACCAATTCT TTCCAGTGTG GTTTCAAGTA TCACAATACC AACAGTTGTC 
TCAGTATCAA CAACATCCCA GTCAGTTCAG GCTCCCACAT CTGGGAGCGT TGTTTCTAGT 
ATAGGCACTT TCCCTTCTAT ACCAGTTTCA GCAACTTCAG CCTCTGCAGT GGGCAGTGCT 
GCTGCCCCAG GGGCTAAGTC TCCACCTTTG TTATCTGAGC AGTCAGCAGG CATTACTACA 
GGGATAGCCA CATTAACATC AGTTTCTACC ACCACTCCAT TCCCAAGCAC AGCTTCACAG 
CTATCTCTTC AGCTTAGCAG CAGCACCTCT GCTCCTACTC TAGCTGAAAC AGTGGTGGTT 
AGTGCACACT CACTAGATAA AACATCTCAT AGCAGTACAA CTGGACTGGC TTTGTCCCAC 
TCTGCATCAT CTTCCACTTC TTCCCCTGGA GCAGGAGTAT CTAGTTCTAT TTCTCAACCT 
GGTGGATTAC ATCCTTTGGT CATCCCATCA GCTGTAGTTT CTACTCCTGT CCTTTCCCAA 
GCATCAGGAC CTATTTCTAC CCCTTTATTA CCCCAAGGAC CTAGCATCCC ACCATTGGTG 
CAACCTGTTG CCAATGTGCC TGCTGTACAG CAGACACTAA TTCATAGTCA GCCTCAACCA 
GCTTTGCTTC TCAACCAGCC CCATACGCAC TGCCCTGAAA TAGATGCTGA TACACAACCC 
AAAGCTCCTG GGATTGATGA CATAAAGACT CTAGAGGAAA AGTTGCGGTC TCTCTTTAGT 
GAACACAGCT CATCTGGCGC TCAACATGCC TCTGTTGCGC TGGAGACATC ACTAGTAGTA 
GAGACCAGTG TCACACCAGG CATCCCAACC ACTGCTGTTG CACCCAGCAA ACTCATGACT 
TCAACTACAA GTACTTGCTT ACCACCAACC AGTTTGCCAT TAGGAACAGC TGGTTTGTCC 
ATTACACCAC TGGTCACAAC TGGGCAAGTT TCTACCCCAG TCAGCACTAC ATCAGGAGTG 
AAACCTGGAA CTGCTCCATC AAAGCTACCT TTAACTAAGG CTCCAGTGCT GCCAGTGGGT 
ACTGAACTTC CAGCTGGTAC TCCATCCAGT GAGCAGCTGC CACCTATTCC AGGACCTTCA 
CTAACTCAGT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTAAGAAG AGCACTTAGT 
CCAGAGGCTA TTGCGGTGAC ATCCGCAGTT GGTCCTGTGT CCGTGGTGGC TCCAGCGGCA 
GTTACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AAGTCACAGA AGGTTCTGTC 
CCAGCAGCTA GTTCAGGAAC TGGTGTTTTT AAGACGGGGC GATTTCAGGT TTCAGTTGCA 
ATAGATGATT CCCAGAAAGA GGGTAAAACT AAGTCAGAAG ATGCAAAGTC TGTTCATTTT 
GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAGAG TACCCTGGTG 
AAGCCAGACT CTAATGGGGT AACCATCCAT GGCATCTCTT CAGATATGCC AGATAGTGCC 
CACAAAACTC CTGCCGCAGA AGCAAAGTCA GAAACTGGGC AACCTACCAA GGTTGGGCGT 
TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTATCAAG AACTGAAGAC 
AGGCTCACTG AGGCAAAGAA AGAGGGACCG ACAGCATCTC CTCCTTTTAT GGATTTGGAA 
CAACCTGTTC TTCCTGCTGC AATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA 
CATCTGAATG GGCCATCTTC TGATTTGGAG GCTGCCTTCC TAAGTAGGGA TGTAGATGAT 
GGTTCAGGTA GTCCACACTC CCCCCACCAA CTGAGCTCAA AGAGCCTACC TATCCAGAAT 
CTAAGTCAAA GCCTTAGTAA CTCACTCAAC TCCTCTTATA TGAGTAGTGA CAATGAGTCA 
GATATTGAAG ATGAAGACTT AAAGTTAGAG CTGCGACGAC TACGAGACAA ACATCTTAAA 
GAAATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG 
GGCAAGGTTC CTCCTGCTGT CATTATTCCC CCAGCGGCCC CCCTTTCAGG AAGAAGAAGG 
CGACCCACTA AAAGCAAAGG CAGCAAATCT AGTCGTATCA GTTCCTTGGG GAGTAAAAGC 
CCCCAGCTTT CAGGTAATAT GTCTGGTCAG AGTGAAGCTT CAGTCTTACA CCCCCAGCAG 
ACCCTCCACC CTCCAGGCAA CATCCCAGAA ACTGGGCAGA ATCAGTTGTT ACAGCCCCTT 
AAGCCATCTC CCTCCAGTGA CAACCTGTAT TCAGCCTTCA CCAGTGATGG TGCCATTTCA 
GTACCAAGCC TTTCTGCTCC AGGTCAAGGG ACCAGCAGCA CAAACACTGT CGGGGGAACA 
GTGAATAGCC AAGCTGCCCA AGCTCAGCCT CCTGCCATGA CGTCCAGCAG GAAGGGCACG 
TTCACAGATG ACCTGCACAA GTTGGTAGAC AATTGGGCCA GAGATGCCAT GAATCTTTCA 
GGCAGGAGAG GAAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC 
TCTGCACCTG GTCAGCTGTG CATCTCCATG ACCTCAAATC TGGGTGGCTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TATGGTTTTC CACCTACCCC CTTTGGCACT CAGTGGAGTG GGACAGGTGG CCCAGCACCA 
CAGCCACTTG GCCAGTTCCA ACCTGTGGGA ACCACCTCCT TACAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011366129.1
CDS	662..7075
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHPQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQSTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPEAIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011367827.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011367827.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCATGGAAT ACAGCAGACA GCCCCTCCTC AACAGACAGT ACAGTATTCA 
CTTCCACAGA CATCAACCTC CAGTGAGGCC ACTACTGCAC AGCCAGTGAG TCAGCCTCAG 
GCTCCACAGG TCTTGCCTCA AGCATCAGCT GGAAAACAGA GTACTCAGGG AGTCTCTCAG 
GTTGCTCCTC CAGAGCCAGT TTCGGTAGCA CAGCCACAGC CCACCCAGCC TGCCACTTTG 
GTCTCTTCTA TAGATAGTGC ACATTCAGAT GTTGCTTCAG GTATGAGCGA TGGCAATGAG 
AATGTGCCAT CATCTGGTGG AAGGCATGAA GGGAGAACTG CAAAACGGCA TTATCGAAAA 
TCTGTAAGAA GTCGCTCTCG TCATGAAAAG ACTTCACGCC CCAAATTGAG AATTTTGAAT 
GTTTCAAATA AAGGAGACCG GGTAGTAGAA TGTCAGTTAG AGACTCACAA TCGGAAAATG 
GTTACATTCA AATTTGATTT AGATGGTGAC AATCCCGAGG AGATAGCAAC AATTATGGTG 
AACAATGACT TTATTCTAGC AATAGAGAGA GAGTCGTTTG TGGATCAAGT ACGGGAAATT 
ATTGAAAAAG CTGATGAAAT GCTCAGTGAG GATGTCAGTG TGGAACCCGA GGGTGACCAG 
GGATTGGAGA GTCTACAGGG AAAGGATGAC TATGGCTTTT CAGGTTCTCA GAAATTGGAA 
GGAGAGTTCA AACAACCACT TCCTGCATCT TCCATGCCAC AGCAAATAGG CATTCCTACC 
AGTTCTTTAA CTCAAGTTGT TCATTCTGCT GGAAGACGGT TTATAGTGAG TCCTGTACCA 
GAAAGCCGAT TACGAGAATC AAAAATTTTC ACCAGTGAAA TATCAGATAC GGTTGCCGCC 
TCTACATCTC ATGGCCCTGG GGTGAACTTG TCCCACTCTG CTTCTTCCCT TAGTCTACAG 
CAGGCCTTTT CTGAACTTAG ACATGCCCAA GTTGCAGAGG GACCCAATAC AGCACCTCCC 
AACTTCAGTC ATATAGGACC AACATTTCCA GTAGTACCTC CTTCCATGAG TAGCATTGCT 
GGAATCCCAA CGACAGCAGC AGCCGTACCT TCAATATCAG TCCCTGTAAC TAGCTGCCCT 
CTTAATGACA TTTCCACATC AATAATTCAA TCTGAGATTA CAGTGCCCAC TGAAAAAGGA 
GTTGCTGGAG TTGCCACCTG CACAGGTGTA ATAACTTCAA GTGGTTTCCC AGTACCACCT 
GCGTCTGAAT CACCAATTCT TTCCAGTGTG GTTTCAAGTA TCACAATACC AACAGTTGTC 
TCAGTATCAA CAACATCCCA GTCAGTTCAG GCTCCCACAT CTGGGAGCGT TGTTTCTAGT 
ATAGGCACTT TCCCTTCTAT ACCAGTTTCA GCAACTTCAG CCTCTGCAGT GGGCAGTGCT 
GCTGCCCCAG GGGCTAAGTC TCCACCTTTG TTATCTGAGC AGTCAGCAGG CATTACTACA 
GGGATAGCCA CATTAACATC AGTTTCTACC ACCACTCCAT TCCCAAGCAC AGCTTCACAG 
CTATCTCTTC AGCTTAGCAG CAGCACCTCT GCTCCTACTC TAGCTGAAAC AGTGGTGGTT 
AGTGCACACT CACTAGATAA AACATCTCAT AGCAGTACAA CTGGACTGGC TTTGTCCCAC 
TCTGCATCAT CTTCCACTTC TTCCCCTGGA GCAGGAGTAT CTAGTTCTAT TTCTCAACCT 
GGTGGATTAC ATCCTTTGGT CATCCCATCA GCTGTAGTTT CTACTCCTGT CCTTTCCCAA 
GCATCAGGAC CTATTTCTAC CCCTTTATTA CCCCAAGGAC CTAGCATCCC ACCATTGGTG 
CAACCTGTTG CCAATGTGCC TGCTGTACAG CAGACACTAA TTCATAGTCA GCCTCAACCA 
GCTTTGCTTC TCAACCAGCC CCATACGCAC TGCCCTGAAA TAGATGCTGA TACACAACCC 
AAAGCTCCTG GGATTGATGA CATAAAGACT CTAGAGGAAA AGTTGCGGTC TCTCTTTAGT 
GAACACAGCT CATCTGGCGC TCAACATGCC TCTGTTGCGC TGGAGACATC ACTAGTAGTA 
GAGACCAGTG TCACACCAGG CATCCCAACC ACTGCTGTTG CACCCAGCAA ACTCATGACT 
TCAACTACAA GTACTTGCTT ACCACCAACC AGTTTGCCAT TAGGAACAGC TGGTTTGTCC 
ATTACACCAC TGGTCACAAC TGGGCAAGTT TCTACCCCAG TCAGCACTAC ATCAGGAGTG 
AAACCTGGAA CTGCTCCATC AAAGCTACCT TTAACTAAGG CTCCAGTGCT GCCAGTGGGT 
ACTGAACTTC CAGCTGGTAC TCCATCCAGT GAGCAGCTGC CACCTATTCC AGGACCTTCA 
CTAACTCAGT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTAAGAAG AGCACTTAGT 
CCAGAGGCTA TTGCGGTGAC ATCCGCAGTT GGTCCTGTGT CCGTGGTGGC TCCAGCGGCA 
GTTACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AAGTCACAGA AGGTTCTGTC 
CCAGCAGCTA GTTCAGGAAC TGGTGTTTTT AAGACGGGGC GATTTCAGGT TTCAGTTGCA 
ATAGATGATT CCCAGAAAGA GGGTAAAACT AAGTCAGAAG ATGCAAAGTC TGTTCATTTT 
GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAGAG TACCCTGGTG 
AAGCCAGACT CTAATGGGGT AACCATCCAT GGCATCTCTT CAGATATGCC AGATAGTGCC 
CACAAAACTC CTGCCGCAGA AGCAAAGTCA GAAACTGGGC AACCTACCAA GGTTGGGCGT 
TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTATCAAG AACTGAAGAC 
AGGCTCACTG AGGCAAAGAA AGAGGGACCG ACAGCATCTC CTCCTTTTAT GGATTTGGAA 
CAACCTGTTC TTCCTGCTGC AATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA 
CATCTGAATG GGCCATCTTC TGATTTGGAG GCTGCCTTCC TAAGTAGGGA TGTAGATGAT 
GGTTCAGGTA GTCCACACTC CCCCCACCAA CTGAGCTCAA AGAGCCTACC TATCCAGAAT 
CTAAGTCAAA GCCTTAGTAA CTCACTCAAC TCCTCTTATA TGAGTAGTGA CAATGAGTCA 
GATATTGAAG ATGAAGACTT AAAGTTAGAG CTGCGACGAC TACGAGACAA ACATCTTAAA 
GAAATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG 
GGCAAGGTTC CTCCTGCTGT CATTATTCCC CCAGCGGCCC CCCTTTCAGG AAGAAGAAGG 
CGACCCACTA AAAGCAAAGG CAGCAAATCT AGTCGTATCA GTTCCTTGGG GAGTAAAAGC 
CCCCAGCTTT CAGGTAATAT GTCTGGTCAG AGTGAAGCTT CAGTCTTACA CCCCCAGCAG 
ACCCTCCACC CTCCAGGCAA CATCCCAGAA ACTGGGCAGA ATCAGTTGTT ACAGCCCCTT 
AAGCCATCTC CCTCCAGTGA CAACCTGTAT TCAGCCTTCA CCAGTGATGG TGCCATTTCA 
GTACCAAGCC TTTCTGCTCC AGGTCAAGGG ACCAGCAGCA CAAACACTGT CGGGGGAACA 
GTGAATAGCC AAGCTGCCCA AGCTCAGCCT CCTGCCATGA CGTCCAGCAG GAAGGGCACG 
TTCACAGATG ACCTGCACAA GTTGGTAGAC AATTGGGCCA GAGATGCCAT GAATCTTTCA 
GGCAGGAGAG GAAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC 
TCTGCACCTG GTCAGCTGTG CATCTCCATG ACCTCAAATC TGGGTGGCTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TATGGTTTTC CACCTACCCC CTTTGGCACT CAGTGGAGTG GGACAGGTGG CCCAGCACCA 
CAGCCACTTG GCCAGTTCCA ACCTGTGGGA ACCACCTCCT TACAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011367828.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011366130.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

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CloneID	OPt52299
Clone ID Related Accession (Same CDS sequence)	XM_011367828.1 , XM_011367828.2
Accession Version	XM_011367828.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCACT 
TTGGTCTCTT CTATAGATAG TGCACATTCA GATGTTGCTT CAGGTATGAG CGATGGCAAT 
GAGAATGTGC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGTCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAGT TAGAGACTCA CAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCCG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCGT TTGTGGATCA AGTACGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC CGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAAGGAGAGT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGCATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCTGGAAGAC GGTTTATAGT GAGTCCTGTA 
CCAGAAAGCC GATTACGAGA ATCAAAAATT TTCACCAGTG AAATATCAGA TACGGTTGCC 
GCCTCTACAT CTCATGGCCC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT TAGACATGCC CAAGTTGCAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATATAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAATCC CAACGACAGC AGCAGCCGTA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACATTTCCAC ATCAATAATT CAATCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCA 
CCTGCGTCTG AATCACCAAT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCAACAGTT 
GTCTCAGTAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTATAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCAGT 
GCTGCTGCCC CAGGGGCTAA GTCTCCACCT TTGTTATCTG AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCACTC CATTCCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCACTAGA TAAAACATCT CATAGCAGTA CAACTGGACT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAA 
CCTGGTGGAT TACATCCTTT GGTCATCCCA TCAGCTGTAG TTTCTACTCC TGTCCTTTCC 
CAAGCATCAG GACCTATTTC TACCCCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCTCAACCA GCCCCATACG CACTGCCCTG AAATAGATGC TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTCTAGAGG AAAAGTTGCG GTCTCTCTTT 
AGTGAACACA GCTCATCTGG CGCTCAACAT GCCTCTGTTG CGCTGGAGAC ATCACTAGTA 
GTAGAGACCA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCATTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CAGTCAGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGCCTGTGTC CGTGGTGGCT CCAGCGGCAG TTACAGAAGC AGGAGCACAG 
CCTCAGAAGG ATGTTTCTCA AGTCACAGAA GGTTCTGTCC CAGCAGCTAG TTCAGGAACT 
GGTGTTTTTA AGACGGGGCG ATTTCAGGTT TCAGTTGCAA TAGATGATTC CCAGAAAGAG 
GGTAAAACTA AGTCAGAAGA TGCAAAGTCT GTTCATTTTG AATCCAGCAC TTCAGAGTCC 
TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT ACCCTGGTGA AGCCAGACTC TAATGGGGTA 
ACCATCCATG GCATCTCTTC AGATATGCCA GATAGTGCCC ACAAAACTCC TGCCGCAGAA 
GCAAAGTCAG AAACTGGGCA ACCTACCAAG GTTGGGCGTT TTCAGGTGAC AACTACAGCA 
AACAAAGTAG GTCGTTTCTC TGTATCAAGA ACTGAAGACA GGCTCACTGA GGCAAAGAAA 
GAGGGACCGA CAGCATCTCC TCCTTTTATG GATTTGGAAC AACCTGTTCT TCCTGCTGCA 
ATACCAAAGA AAGAAAAGCC TGAACTGTCA GAGCCTTCAC ATCTGAATGG GCCATCTTCT 
GATTTGGAGG CTGCCTTCCT AAGTAGGGAT GTAGATGATG GTTCAGGTAG TCCACACTCC 
CCCCACCAAC TGAGCTCAAA GAGCCTACCT ATCCAGAATC TAAGTCAAAG CCTTAGTAAC 
TCACTCAACT CCTCTTATAT GAGTAGTGAC AATGAGTCAG ATATTGAAGA TGAAGACTTA 
AAGTTAGAGC TGCGACGACT ACGAGACAAA CATCTTAAAG AAATTCAAGA CCTGCAGAGT 
CGCCAGAAGC ATGAAATTGA ATCTTTGTAT ACTAAACTGG GCAAGGTTCC TCCTGCTGTC 
ATTATTCCCC CAGCGGCCCC CCTTTCAGGA AGAAGAAGGC GACCCACTAA AAGCAAAGGC 
AGCAAATCTA GTCGTATCAG TTCCTTGGGG AGTAAAAGCC CCCAGCTTTC AGGTAATATG 
TCTGGTCAGA GTGAAGCTTC AGTCTTACAC CCCCAGCAGA CCCTCCACCC TCCAGGCAAC 
ATCCCAGAAA CTGGGCAGAA TCAGTTGTTA CAGCCCCTTA AGCCATCTCC CTCCAGTGAC 
AACCTGTATT CAGCCTTCAC CAGTGATGGT GCCATTTCAG TACCAAGCCT TTCTGCTCCA 
GGTCAAGGGA CCAGCAGCAC AAACACTGTC GGGGGAACAG TGAATAGCCA AGCTGCCCAA 
GCTCAGCCTC CTGCCATGAC GTCCAGCAGG AAGGGCACGT TCACAGATGA CCTGCACAAG 
TTGGTAGACA ATTGGGCCAG AGATGCCATG AATCTTTCAG GCAGGAGAGG AAGCAAAGGA 
CACATGAATT ATGAGGGCCC TGGAATGGCA AGGAAGTTCT CTGCACCTGG TCAGCTGTGC 
ATCTCCATGA CCTCAAATCT GGGTGGCTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT 
CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ATGGTTTTCC ACCTACCCCC 
TTTGGCACTC AGTGGAGTGG GACAGGTGGC CCAGCACCAC AGCCACTTGG CCAGTTCCAA 
CCTGTGGGAA CCACCTCCTT ACAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC 
AACCCCCCAG GTTCCAACCT GCGGACCACT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011366130.1
CDS	1011..7343
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHPQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQSTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011367828.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011367828.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCACT 
TTGGTCTCTT CTATAGATAG TGCACATTCA GATGTTGCTT CAGGTATGAG CGATGGCAAT 
GAGAATGTGC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGTCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAGT TAGAGACTCA CAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCCG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCGT TTGTGGATCA AGTACGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC CGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAAGGAGAGT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGCATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCTGGAAGAC GGTTTATAGT GAGTCCTGTA 
CCAGAAAGCC GATTACGAGA ATCAAAAATT TTCACCAGTG AAATATCAGA TACGGTTGCC 
GCCTCTACAT CTCATGGCCC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT TAGACATGCC CAAGTTGCAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATATAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAATCC CAACGACAGC AGCAGCCGTA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACATTTCCAC ATCAATAATT CAATCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCA 
CCTGCGTCTG AATCACCAAT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCAACAGTT 
GTCTCAGTAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTATAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCAGT 
GCTGCTGCCC CAGGGGCTAA GTCTCCACCT TTGTTATCTG AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCACTC CATTCCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCACTAGA TAAAACATCT CATAGCAGTA CAACTGGACT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAA 
CCTGGTGGAT TACATCCTTT GGTCATCCCA TCAGCTGTAG TTTCTACTCC TGTCCTTTCC 
CAAGCATCAG GACCTATTTC TACCCCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCTCAACCA GCCCCATACG CACTGCCCTG AAATAGATGC TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTCTAGAGG AAAAGTTGCG GTCTCTCTTT 
AGTGAACACA GCTCATCTGG CGCTCAACAT GCCTCTGTTG CGCTGGAGAC ATCACTAGTA 
GTAGAGACCA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCATTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CAGTCAGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGCCTGTGTC CGTGGTGGCT CCAGCGGCAG TTACAGAAGC AGGAGCACAG 
CCTCAGAAGG ATGTTTCTCA AGTCACAGAA GGTTCTGTCC CAGCAGCTAG TTCAGGAACT 
GGTGTTTTTA AGACGGGGCG ATTTCAGGTT TCAGTTGCAA TAGATGATTC CCAGAAAGAG 
GGTAAAACTA AGTCAGAAGA TGCAAAGTCT GTTCATTTTG AATCCAGCAC TTCAGAGTCC 
TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT ACCCTGGTGA AGCCAGACTC TAATGGGGTA 
ACCATCCATG GCATCTCTTC AGATATGCCA GATAGTGCCC ACAAAACTCC TGCCGCAGAA 
GCAAAGTCAG AAACTGGGCA ACCTACCAAG GTTGGGCGTT TTCAGGTGAC AACTACAGCA 
AACAAAGTAG GTCGTTTCTC TGTATCAAGA ACTGAAGACA GGCTCACTGA GGCAAAGAAA 
GAGGGACCGA CAGCATCTCC TCCTTTTATG GATTTGGAAC AACCTGTTCT TCCTGCTGCA 
ATACCAAAGA AAGAAAAGCC TGAACTGTCA GAGCCTTCAC ATCTGAATGG GCCATCTTCT 
GATTTGGAGG CTGCCTTCCT AAGTAGGGAT GTAGATGATG GTTCAGGTAG TCCACACTCC 
CCCCACCAAC TGAGCTCAAA GAGCCTACCT ATCCAGAATC TAAGTCAAAG CCTTAGTAAC 
TCACTCAACT CCTCTTATAT GAGTAGTGAC AATGAGTCAG ATATTGAAGA TGAAGACTTA 
AAGTTAGAGC TGCGACGACT ACGAGACAAA CATCTTAAAG AAATTCAAGA CCTGCAGAGT 
CGCCAGAAGC ATGAAATTGA ATCTTTGTAT ACTAAACTGG GCAAGGTTCC TCCTGCTGTC 
ATTATTCCCC CAGCGGCCCC CCTTTCAGGA AGAAGAAGGC GACCCACTAA AAGCAAAGGC 
AGCAAATCTA GTCGTATCAG TTCCTTGGGG AGTAAAAGCC CCCAGCTTTC AGGTAATATG 
TCTGGTCAGA GTGAAGCTTC AGTCTTACAC CCCCAGCAGA CCCTCCACCC TCCAGGCAAC 
ATCCCAGAAA CTGGGCAGAA TCAGTTGTTA CAGCCCCTTA AGCCATCTCC CTCCAGTGAC 
AACCTGTATT CAGCCTTCAC CAGTGATGGT GCCATTTCAG TACCAAGCCT TTCTGCTCCA 
GGTCAAGGGA CCAGCAGCAC AAACACTGTC GGGGGAACAG TGAATAGCCA AGCTGCCCAA 
GCTCAGCCTC CTGCCATGAC GTCCAGCAGG AAGGGCACGT TCACAGATGA CCTGCACAAG 
TTGGTAGACA ATTGGGCCAG AGATGCCATG AATCTTTCAG GCAGGAGAGG AAGCAAAGGA 
CACATGAATT ATGAGGGCCC TGGAATGGCA AGGAAGTTCT CTGCACCTGG TCAGCTGTGC 
ATCTCCATGA CCTCAAATCT GGGTGGCTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT 
CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ATGGTTTTCC ACCTACCCCC 
TTTGGCACTC AGTGGAGTGG GACAGGTGGC CCAGCACCAC AGCCACTTGG CCAGTTCCAA 
CCTGTGGGAA CCACCTCCTT ACAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC 
AACCCCCCAG GTTCCAACCT GCGGACCACT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011367828.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011366130.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

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CloneID	OPt52299
Clone ID Related Accession (Same CDS sequence)	XM_011367828.1 , XM_011367828.2
Accession Version	XM_011367828.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011367828.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCACT 
TTGGTCTCTT CTATAGATAG TGCACATTCA GATGTTGCTT CAGGTATGAG CGATGGCAAT 
GAGAATGTGC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGTCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAGT TAGAGACTCA CAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCCG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCGT TTGTGGATCA AGTACGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC CGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAAGGAGAGT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGCATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCTGGAAGAC GGTTTATAGT GAGTCCTGTA 
CCAGAAAGCC GATTACGAGA ATCAAAAATT TTCACCAGTG AAATATCAGA TACGGTTGCC 
GCCTCTACAT CTCATGGCCC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT TAGACATGCC CAAGTTGCAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATATAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAATCC CAACGACAGC AGCAGCCGTA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACATTTCCAC ATCAATAATT CAATCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCA 
CCTGCGTCTG AATCACCAAT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCAACAGTT 
GTCTCAGTAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTATAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCAGT 
GCTGCTGCCC CAGGGGCTAA GTCTCCACCT TTGTTATCTG AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCACTC CATTCCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCACTAGA TAAAACATCT CATAGCAGTA CAACTGGACT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAA 
CCTGGTGGAT TACATCCTTT GGTCATCCCA TCAGCTGTAG TTTCTACTCC TGTCCTTTCC 
CAAGCATCAG GACCTATTTC TACCCCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCTCAACCA GCCCCATACG CACTGCCCTG AAATAGATGC TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTCTAGAGG AAAAGTTGCG GTCTCTCTTT 
AGTGAACACA GCTCATCTGG CGCTCAACAT GCCTCTGTTG CGCTGGAGAC ATCACTAGTA 
GTAGAGACCA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCATTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CAGTCAGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGCCTGTGTC CGTGGTGGCT CCAGCGGCAG TTACAGAAGC AGGAGCACAG 
CCTCAGAAGG ATGTTTCTCA AGTCACAGAA GGTTCTGTCC CAGCAGCTAG TTCAGGAACT 
GGTGTTTTTA AGACGGGGCG ATTTCAGGTT TCAGTTGCAA TAGATGATTC CCAGAAAGAG 
GGTAAAACTA AGTCAGAAGA TGCAAAGTCT GTTCATTTTG AATCCAGCAC TTCAGAGTCC 
TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT ACCCTGGTGA AGCCAGACTC TAATGGGGTA 
ACCATCCATG GCATCTCTTC AGATATGCCA GATAGTGCCC ACAAAACTCC TGCCGCAGAA 
GCAAAGTCAG AAACTGGGCA ACCTACCAAG GTTGGGCGTT TTCAGGTGAC AACTACAGCA 
AACAAAGTAG GTCGTTTCTC TGTATCAAGA ACTGAAGACA GGCTCACTGA GGCAAAGAAA 
GAGGGACCGA CAGCATCTCC TCCTTTTATG GATTTGGAAC AACCTGTTCT TCCTGCTGCA 
ATACCAAAGA AAGAAAAGCC TGAACTGTCA GAGCCTTCAC ATCTGAATGG GCCATCTTCT 
GATTTGGAGG CTGCCTTCCT AAGTAGGGAT GTAGATGATG GTTCAGGTAG TCCACACTCC 
CCCCACCAAC TGAGCTCAAA GAGCCTACCT ATCCAGAATC TAAGTCAAAG CCTTAGTAAC 
TCACTCAACT CCTCTTATAT GAGTAGTGAC AATGAGTCAG ATATTGAAGA TGAAGACTTA 
AAGTTAGAGC TGCGACGACT ACGAGACAAA CATCTTAAAG AAATTCAAGA CCTGCAGAGT 
CGCCAGAAGC ATGAAATTGA ATCTTTGTAT ACTAAACTGG GCAAGGTTCC TCCTGCTGTC 
ATTATTCCCC CAGCGGCCCC CCTTTCAGGA AGAAGAAGGC GACCCACTAA AAGCAAAGGC 
AGCAAATCTA GTCGTATCAG TTCCTTGGGG AGTAAAAGCC CCCAGCTTTC AGGTAATATG 
TCTGGTCAGA GTGAAGCTTC AGTCTTACAC CCCCAGCAGA CCCTCCACCC TCCAGGCAAC 
ATCCCAGAAA CTGGGCAGAA TCAGTTGTTA CAGCCCCTTA AGCCATCTCC CTCCAGTGAC 
AACCTGTATT CAGCCTTCAC CAGTGATGGT GCCATTTCAG TACCAAGCCT TTCTGCTCCA 
GGTCAAGGGA CCAGCAGCAC AAACACTGTC GGGGGAACAG TGAATAGCCA AGCTGCCCAA 
GCTCAGCCTC CTGCCATGAC GTCCAGCAGG AAGGGCACGT TCACAGATGA CCTGCACAAG 
TTGGTAGACA ATTGGGCCAG AGATGCCATG AATCTTTCAG GCAGGAGAGG AAGCAAAGGA 
CACATGAATT ATGAGGGCCC TGGAATGGCA AGGAAGTTCT CTGCACCTGG TCAGCTGTGC 
ATCTCCATGA CCTCAAATCT GGGTGGCTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT 
CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ATGGTTTTCC ACCTACCCCC 
TTTGGCACTC AGTGGAGTGG GACAGGTGGC CCAGCACCAC AGCCACTTGG CCAGTTCCAA 
CCTGTGGGAA CCACCTCCTT ACAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC 
AACCCCCCAG GTTCCAACCT GCGGACCACT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011366130.1
CDS	661..6993
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHPQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQSTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011367828.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011367828.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCACT 
TTGGTCTCTT CTATAGATAG TGCACATTCA GATGTTGCTT CAGGTATGAG CGATGGCAAT 
GAGAATGTGC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGTCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAGT TAGAGACTCA CAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCCG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCGT TTGTGGATCA AGTACGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC CGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAAGGAGAGT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGCATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCTGGAAGAC GGTTTATAGT GAGTCCTGTA 
CCAGAAAGCC GATTACGAGA ATCAAAAATT TTCACCAGTG AAATATCAGA TACGGTTGCC 
GCCTCTACAT CTCATGGCCC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT TAGACATGCC CAAGTTGCAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATATAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAATCC CAACGACAGC AGCAGCCGTA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACATTTCCAC ATCAATAATT CAATCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCA 
CCTGCGTCTG AATCACCAAT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCAACAGTT 
GTCTCAGTAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTATAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCAGT 
GCTGCTGCCC CAGGGGCTAA GTCTCCACCT TTGTTATCTG AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCACTC CATTCCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCACTAGA TAAAACATCT CATAGCAGTA CAACTGGACT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAA 
CCTGGTGGAT TACATCCTTT GGTCATCCCA TCAGCTGTAG TTTCTACTCC TGTCCTTTCC 
CAAGCATCAG GACCTATTTC TACCCCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCTCAACCA GCCCCATACG CACTGCCCTG AAATAGATGC TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTCTAGAGG AAAAGTTGCG GTCTCTCTTT 
AGTGAACACA GCTCATCTGG CGCTCAACAT GCCTCTGTTG CGCTGGAGAC ATCACTAGTA 
GTAGAGACCA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCATTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CAGTCAGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGCCTGTGTC CGTGGTGGCT CCAGCGGCAG TTACAGAAGC AGGAGCACAG 
CCTCAGAAGG ATGTTTCTCA AGTCACAGAA GGTTCTGTCC CAGCAGCTAG TTCAGGAACT 
GGTGTTTTTA AGACGGGGCG ATTTCAGGTT TCAGTTGCAA TAGATGATTC CCAGAAAGAG 
GGTAAAACTA AGTCAGAAGA TGCAAAGTCT GTTCATTTTG AATCCAGCAC TTCAGAGTCC 
TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT ACCCTGGTGA AGCCAGACTC TAATGGGGTA 
ACCATCCATG GCATCTCTTC AGATATGCCA GATAGTGCCC ACAAAACTCC TGCCGCAGAA 
GCAAAGTCAG AAACTGGGCA ACCTACCAAG GTTGGGCGTT TTCAGGTGAC AACTACAGCA 
AACAAAGTAG GTCGTTTCTC TGTATCAAGA ACTGAAGACA GGCTCACTGA GGCAAAGAAA 
GAGGGACCGA CAGCATCTCC TCCTTTTATG GATTTGGAAC AACCTGTTCT TCCTGCTGCA 
ATACCAAAGA AAGAAAAGCC TGAACTGTCA GAGCCTTCAC ATCTGAATGG GCCATCTTCT 
GATTTGGAGG CTGCCTTCCT AAGTAGGGAT GTAGATGATG GTTCAGGTAG TCCACACTCC 
CCCCACCAAC TGAGCTCAAA GAGCCTACCT ATCCAGAATC TAAGTCAAAG CCTTAGTAAC 
TCACTCAACT CCTCTTATAT GAGTAGTGAC AATGAGTCAG ATATTGAAGA TGAAGACTTA 
AAGTTAGAGC TGCGACGACT ACGAGACAAA CATCTTAAAG AAATTCAAGA CCTGCAGAGT 
CGCCAGAAGC ATGAAATTGA ATCTTTGTAT ACTAAACTGG GCAAGGTTCC TCCTGCTGTC 
ATTATTCCCC CAGCGGCCCC CCTTTCAGGA AGAAGAAGGC GACCCACTAA AAGCAAAGGC 
AGCAAATCTA GTCGTATCAG TTCCTTGGGG AGTAAAAGCC CCCAGCTTTC AGGTAATATG 
TCTGGTCAGA GTGAAGCTTC AGTCTTACAC CCCCAGCAGA CCCTCCACCC TCCAGGCAAC 
ATCCCAGAAA CTGGGCAGAA TCAGTTGTTA CAGCCCCTTA AGCCATCTCC CTCCAGTGAC 
AACCTGTATT CAGCCTTCAC CAGTGATGGT GCCATTTCAG TACCAAGCCT TTCTGCTCCA 
GGTCAAGGGA CCAGCAGCAC AAACACTGTC GGGGGAACAG TGAATAGCCA AGCTGCCCAA 
GCTCAGCCTC CTGCCATGAC GTCCAGCAGG AAGGGCACGT TCACAGATGA CCTGCACAAG 
TTGGTAGACA ATTGGGCCAG AGATGCCATG AATCTTTCAG GCAGGAGAGG AAGCAAAGGA 
CACATGAATT ATGAGGGCCC TGGAATGGCA AGGAAGTTCT CTGCACCTGG TCAGCTGTGC 
ATCTCCATGA CCTCAAATCT GGGTGGCTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT 
CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ATGGTTTTCC ACCTACCCCC 
TTTGGCACTC AGTGGAGTGG GACAGGTGGC CCAGCACCAC AGCCACTTGG CCAGTTCCAA 
CCTGTGGGAA CCACCTCCTT ACAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC 
AACCCCCCAG GTTCCAACCT GCGGACCACT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_023535114.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023390882.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

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CloneID	OPt72364
Clone ID Related Accession (Same CDS sequence)	XM_023535114.1
Accession Version	XM_023535114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGCA GCATGGAATA 
CAGCAGACAG CCCCTCCTCA ACAGACAGTA CAGTATTCAC TTCCACAGAC ATCAACCTCC 
AGTGAGGCCA CTACTGCACA GCCAGTGAGT CAGCCTCAGG CTCCACAGGT CTTGCCTCAA 
GCATCAGCTG GAAAACAGCT TCCAGTTTCC CAGCCAGTAC CAACTGTCCA AGGCGAACCT 
CAGATCTCAG TTGCGACACA ACCCTCAGTT GTTCCAGTGC ATTCTGGTGC TCATTTCCTC 
CCTATGGGAC AGCCACTTCC TACTTCCTTA CTCCCTCAGT ACCCTGTCTC TCAAATTCCT 
GTATCAACTC CTCATGTGTC CGCGGCTCAG ACAGGTTTCT CAACCCTTCC CATCACAATT 
GCAGCTGGCC TTAATCAGCC TCTGCTCACC TTGGCTTCAT CTGCTACAGC AGCCGCAATC 
CCAGGGGGAT CAACTATGGC TCCTAGTCAG CTACCAGCCC TTCTGCAGCC TGTGACTCAG 
CTGCCAAGTC AGGTTCACCC ACAGCTCCTG CAGCCAGCAG TTCAGTCCAT GGGAATACCA 
GCTAACCTTG GACAAGCTGC TGAGGTTCCA CTTCCCTCTG GAGATGTTCT ATACCAGGGC 
TTCCCACCTC GACTGCCACC ACAATACCCA GGAGATTCAA ATATTGCTCC CTCTTCCAGC 
GTGGCTTCTG TTTGCATTCC TTCTACAGTC TTATCCCCTC CAATGCCGAC AGAAGCATTA 
GCTACACCAG GTTACTTTCC TACAGTGGTG CAGCCTTATA TGGAATCAAA TCTTTTGGTT 
CCTGTGGGCA GTGTAGGAGG ACAGGTTCAA GTGTCCCAAC CAGCAGTGAG TTTAGCACAA 
GCCCCCACTG CATCCTCCCA GCAAGCAGCT CTGGAGAGTA CTCAGGGAGT CTCTCAGGTT 
GCTCCTCCAG AGCCAGTTTC GGTAGCACAG CCACAGCCCA CCCAGCCTGC CACTTTGGTC 
TCTTCTATAG ATAGTGCACA TTCAGATGTT GCTTCAGGTA TGAGCGATGG CAATGAGAAT 
GTGCCATCAT CTGGTGGAAG GCATGAAGGG AGAACTGCAA AACGGCATTA TCGAAAATCT 
GTAAGAAGTC GCTCTCGTCA TGAAAAGACT TCACGCCCCA AATTGAGAAT TTTGAATGTT 
TCAAATAAAG GAGACCGGGT AGTAGAATGT CAGTTAGAGA CTCACAATCG GAAAATGGTT 
ACATTCAAAT TTGATTTAGA TGGTGACAAT CCCGAGGAGA TAGCAACAAT TATGGTGAAC 
AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCGTTTGTGG ATCAAGTACG GGAAATTATT 
GAAAAAGCTG ATGAAATGCT CAGTGAGGAT GTCAGTGTGG AACCCGAGGG TGACCAGGGA 
TTGGAGAGTC TACAGGGAAA GGATGACTAT GGCTTTTCAG GTTCTCAGAA ATTGGAAGGA 
GAGTTCAAAC AACCACTTCC TGCATCTTCC ATGCCACAGC AAATAGGCAT TCCTACCAGT 
TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCAGAA 
AGCCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAT CAGATACGGT TGCCGCCTCT 
ACATCTCATG GCCCTGGGGT GAACTTGTCC CACTCTGCTT CTTCCCTTAG TCTACAGCAG 
GCCTTTTCTG AACTTAGACA TGCCCAAGTT GCAGAGGGAC CCAATACAGC ACCTCCCAAC 
TTCAGTCATA TAGGACCAAC ATTTCCAGTA GTACCTCCTT CCATGAGTAG CATTGCTGGA 
ATCCCAACGA CAGCAGCAGC CGTACCTTCA ATATCAGTCC CTGTAACTAG CTGCCCTCTT 
AATGACATTT CCACATCAAT AATTCAATCT GAGATTACAG TGCCCACTGA AAAAGGAGTT 
GCTGGAGTTG CCACCTGCAC AGGTGTAATA ACTTCAAGTG GTTTCCCAGT ACCACCTGCG 
TCTGAATCAC CAATTCTTTC CAGTGTGGTT TCAAGTATCA CAATACCAAC AGTTGTCTCA 
GTATCAACAA CATCCCAGTC AGTTCAGGCT CCCACATCTG GGAGCGTTGT TTCTAGTATA 
GGCACTTTCC CTTCTATACC AGTTTCAGCA ACTTCAGCCT CTGCAGTGGG CAGTGCTGCT 
GCCCCAGGGG CTAAGTCTCC ACCTTTGTTA TCTGAGCAGT CAGCAGGCAT TACTACAGGG 
ATAGCCACAT TAACATCAGT TTCTACCACC ACTCCATTCC CAAGCACAGC TTCACAGCTA 
TCTCTTCAGC TTAGCAGCAG CACCTCTGCT CCTACTCTAG CTGAAACAGT GGTGGTTAGT 
GCACACTCAC TAGATAAAAC ATCTCATAGC AGTACAACTG GACTGGCTTT GTCCCACTCT 
GCATCATCTT CCACTTCTTC CCCTGGAGCA GGAGTATCTA GTTCTATTTC TCAACCTGGT 
GGATTACATC CTTTGGTCAT CCCATCAGCT GTAGTTTCTA CTCCTGTCCT TTCCCAAGCA 
TCAGGACCTA TTTCTACCCC TTTATTACCC CAAGGACCTA GCATCCCACC ATTGGTGCAA 
CCTGTTGCCA ATGTGCCTGC TGTACAGCAG ACACTAATTC ATAGTCAGCC TCAACCAGCT 
TTGCTTCTCA ACCAGCCCCA TACGCACTGC CCTGAAATAG ATGCTGATAC ACAACCCAAA 
GCTCCTGGGA TTGATGACAT AAAGACTCTA GAGGAAAAGT TGCGGTCTCT CTTTAGTGAA 
CACAGCTCAT CTGGCGCTCA ACATGCCTCT GTTGCGCTGG AGACATCACT AGTAGTAGAG 
ACCAGTGTCA CACCAGGCAT CCCAACCACT GCTGTTGCAC CCAGCAAACT CATGACTTCA 
ACTACAAGTA CTTGCTTACC ACCAACCAGT TTGCCATTAG GAACAGCTGG TTTGTCCATT 
ACACCACTGG TCACAACTGG GCAAGTTTCT ACCCCAGTCA GCACTACATC AGGAGTGAAA 
CCTGGAACTG CTCCATCAAA GCTACCTTTA ACTAAGGCTC CAGTGCTGCC AGTGGGTACT 
GAACTTCCAG CTGGTACTCC ATCCAGTGAG CAGCTGCCAC CTATTCCAGG ACCTTCACTA 
ACTCAGTCCC AGCAACCTCT GGAAGATCTT GATGCTCAGT TAAGAAGAGC ACTTAGTCCA 
GAGGCTATTG CGGTGACATC CGCAGTTGGT CCTGTGTCCG TGGTGGCTCC AGCGGCAGTT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCACAGAAGG TTCTGTCCCA 
GCAGCTAGTT CAGGAACTGG TGTTTTTAAG ACGGGGCGAT TTCAGGTTTC AGTTGCAATA 
GATGATTCCC AGAAAGAGGG TAAAACTAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CCTGGTGAAG 
CCAGACTCTA ATGGGGTAAC CATCCATGGC ATCTCTTCAG ATATGCCAGA TAGTGCCCAC 
AAAACTCCTG CCGCAGAAGC AAAGTCAGAA ACTGGGCAAC CTACCAAGGT TGGGCGTTTT 
CAGGTGACAA CTACAGCAAA CAAAGTAGGT CGTTTCTCTG TATCAAGAAC TGAAGACAGG 
CTCACTGAGG CAAAGAAAGA GGGACCGACA GCATCTCCTC CTTTTATGGA TTTGGAACAA 
CCTGTTCTTC CTGCTGCAAT ACCAAAGAAA GAAAAGCCTG AACTGTCAGA GCCTTCACAT 
CTGAATGGGC CATCTTCTGA TTTGGAGGCT GCCTTCCTAA GTAGGGATGT AGATGATGGT 
TCAGGTAGTC CACACTCCCC CCACCAACTG AGCTCAAAGA GCCTACCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAACTC ACTCAACTCC TCTTATATGA GTAGTGACAA TGAGTCAGAT 
ATTGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGACAAACA TCTTAAAGAA 
ATTCAAGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC TAAACTGGGC 
AAGGTTCCTC CTGCTGTCAT TATTCCCCCA GCGGCCCCCC TTTCAGGAAG AAGAAGGCGA 
CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGTATCAGTT CCTTGGGGAG TAAAAGCCCC 
CAGCTTTCAG GTAATATGTC TGGTCAGAGT GAAGCTTCAG TCTTACACCC CCAGCAGACC 
CTCCACCCTC CAGGCAACAT CCCAGAAACT GGGCAGAATC AGTTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTGTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG TCAAGGGACC AGCAGCACAA ACACTGTCGG GGGAACAGTG 
AATAGCCAAG CTGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACGTTC 
ACAGATGACC TGCACAAGTT GGTAGACAAT TGGGCCAGAG ATGCCATGAA TCTTTCAGGC 
AGGAGAGGAA GCAAAGGACA CATGAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTCTCT 
GCACCTGGTC AGCTGTGCAT CTCCATGACC TCAAATCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAT 
GGTTTTCCAC CTACCCCCTT TGGCACTCAG TGGAGTGGGA CAGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACC ACCTCCTTAC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023390882.1
CDS	669..7739
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPVSQIPVSTPHVSAAQTGFSTLPITIAAGLNQPLLTLASSATAAAIPGGSTMAPSQLPALLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIAPSSSVASVCIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLLVPVGSVGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPEAIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_023535114.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023535114.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGCA GCATGGAATA 
CAGCAGACAG CCCCTCCTCA ACAGACAGTA CAGTATTCAC TTCCACAGAC ATCAACCTCC 
AGTGAGGCCA CTACTGCACA GCCAGTGAGT CAGCCTCAGG CTCCACAGGT CTTGCCTCAA 
GCATCAGCTG GAAAACAGCT TCCAGTTTCC CAGCCAGTAC CAACTGTCCA AGGCGAACCT 
CAGATCTCAG TTGCGACACA ACCCTCAGTT GTTCCAGTGC ATTCTGGTGC TCATTTCCTC 
CCTATGGGAC AGCCACTTCC TACTTCCTTA CTCCCTCAGT ACCCTGTCTC TCAAATTCCT 
GTATCAACTC CTCATGTGTC CGCGGCTCAG ACAGGTTTCT CAACCCTTCC CATCACAATT 
GCAGCTGGCC TTAATCAGCC TCTGCTCACC TTGGCTTCAT CTGCTACAGC AGCCGCAATC 
CCAGGGGGAT CAACTATGGC TCCTAGTCAG CTACCAGCCC TTCTGCAGCC TGTGACTCAG 
CTGCCAAGTC AGGTTCACCC ACAGCTCCTG CAGCCAGCAG TTCAGTCCAT GGGAATACCA 
GCTAACCTTG GACAAGCTGC TGAGGTTCCA CTTCCCTCTG GAGATGTTCT ATACCAGGGC 
TTCCCACCTC GACTGCCACC ACAATACCCA GGAGATTCAA ATATTGCTCC CTCTTCCAGC 
GTGGCTTCTG TTTGCATTCC TTCTACAGTC TTATCCCCTC CAATGCCGAC AGAAGCATTA 
GCTACACCAG GTTACTTTCC TACAGTGGTG CAGCCTTATA TGGAATCAAA TCTTTTGGTT 
CCTGTGGGCA GTGTAGGAGG ACAGGTTCAA GTGTCCCAAC CAGCAGTGAG TTTAGCACAA 
GCCCCCACTG CATCCTCCCA GCAAGCAGCT CTGGAGAGTA CTCAGGGAGT CTCTCAGGTT 
GCTCCTCCAG AGCCAGTTTC GGTAGCACAG CCACAGCCCA CCCAGCCTGC CACTTTGGTC 
TCTTCTATAG ATAGTGCACA TTCAGATGTT GCTTCAGGTA TGAGCGATGG CAATGAGAAT 
GTGCCATCAT CTGGTGGAAG GCATGAAGGG AGAACTGCAA AACGGCATTA TCGAAAATCT 
GTAAGAAGTC GCTCTCGTCA TGAAAAGACT TCACGCCCCA AATTGAGAAT TTTGAATGTT 
TCAAATAAAG GAGACCGGGT AGTAGAATGT CAGTTAGAGA CTCACAATCG GAAAATGGTT 
ACATTCAAAT TTGATTTAGA TGGTGACAAT CCCGAGGAGA TAGCAACAAT TATGGTGAAC 
AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCGTTTGTGG ATCAAGTACG GGAAATTATT 
GAAAAAGCTG ATGAAATGCT CAGTGAGGAT GTCAGTGTGG AACCCGAGGG TGACCAGGGA 
TTGGAGAGTC TACAGGGAAA GGATGACTAT GGCTTTTCAG GTTCTCAGAA ATTGGAAGGA 
GAGTTCAAAC AACCACTTCC TGCATCTTCC ATGCCACAGC AAATAGGCAT TCCTACCAGT 
TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCAGAA 
AGCCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAT CAGATACGGT TGCCGCCTCT 
ACATCTCATG GCCCTGGGGT GAACTTGTCC CACTCTGCTT CTTCCCTTAG TCTACAGCAG 
GCCTTTTCTG AACTTAGACA TGCCCAAGTT GCAGAGGGAC CCAATACAGC ACCTCCCAAC 
TTCAGTCATA TAGGACCAAC ATTTCCAGTA GTACCTCCTT CCATGAGTAG CATTGCTGGA 
ATCCCAACGA CAGCAGCAGC CGTACCTTCA ATATCAGTCC CTGTAACTAG CTGCCCTCTT 
AATGACATTT CCACATCAAT AATTCAATCT GAGATTACAG TGCCCACTGA AAAAGGAGTT 
GCTGGAGTTG CCACCTGCAC AGGTGTAATA ACTTCAAGTG GTTTCCCAGT ACCACCTGCG 
TCTGAATCAC CAATTCTTTC CAGTGTGGTT TCAAGTATCA CAATACCAAC AGTTGTCTCA 
GTATCAACAA CATCCCAGTC AGTTCAGGCT CCCACATCTG GGAGCGTTGT TTCTAGTATA 
GGCACTTTCC CTTCTATACC AGTTTCAGCA ACTTCAGCCT CTGCAGTGGG CAGTGCTGCT 
GCCCCAGGGG CTAAGTCTCC ACCTTTGTTA TCTGAGCAGT CAGCAGGCAT TACTACAGGG 
ATAGCCACAT TAACATCAGT TTCTACCACC ACTCCATTCC CAAGCACAGC TTCACAGCTA 
TCTCTTCAGC TTAGCAGCAG CACCTCTGCT CCTACTCTAG CTGAAACAGT GGTGGTTAGT 
GCACACTCAC TAGATAAAAC ATCTCATAGC AGTACAACTG GACTGGCTTT GTCCCACTCT 
GCATCATCTT CCACTTCTTC CCCTGGAGCA GGAGTATCTA GTTCTATTTC TCAACCTGGT 
GGATTACATC CTTTGGTCAT CCCATCAGCT GTAGTTTCTA CTCCTGTCCT TTCCCAAGCA 
TCAGGACCTA TTTCTACCCC TTTATTACCC CAAGGACCTA GCATCCCACC ATTGGTGCAA 
CCTGTTGCCA ATGTGCCTGC TGTACAGCAG ACACTAATTC ATAGTCAGCC TCAACCAGCT 
TTGCTTCTCA ACCAGCCCCA TACGCACTGC CCTGAAATAG ATGCTGATAC ACAACCCAAA 
GCTCCTGGGA TTGATGACAT AAAGACTCTA GAGGAAAAGT TGCGGTCTCT CTTTAGTGAA 
CACAGCTCAT CTGGCGCTCA ACATGCCTCT GTTGCGCTGG AGACATCACT AGTAGTAGAG 
ACCAGTGTCA CACCAGGCAT CCCAACCACT GCTGTTGCAC CCAGCAAACT CATGACTTCA 
ACTACAAGTA CTTGCTTACC ACCAACCAGT TTGCCATTAG GAACAGCTGG TTTGTCCATT 
ACACCACTGG TCACAACTGG GCAAGTTTCT ACCCCAGTCA GCACTACATC AGGAGTGAAA 
CCTGGAACTG CTCCATCAAA GCTACCTTTA ACTAAGGCTC CAGTGCTGCC AGTGGGTACT 
GAACTTCCAG CTGGTACTCC ATCCAGTGAG CAGCTGCCAC CTATTCCAGG ACCTTCACTA 
ACTCAGTCCC AGCAACCTCT GGAAGATCTT GATGCTCAGT TAAGAAGAGC ACTTAGTCCA 
GAGGCTATTG CGGTGACATC CGCAGTTGGT CCTGTGTCCG TGGTGGCTCC AGCGGCAGTT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCACAGAAGG TTCTGTCCCA 
GCAGCTAGTT CAGGAACTGG TGTTTTTAAG ACGGGGCGAT TTCAGGTTTC AGTTGCAATA 
GATGATTCCC AGAAAGAGGG TAAAACTAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CCTGGTGAAG 
CCAGACTCTA ATGGGGTAAC CATCCATGGC ATCTCTTCAG ATATGCCAGA TAGTGCCCAC 
AAAACTCCTG CCGCAGAAGC AAAGTCAGAA ACTGGGCAAC CTACCAAGGT TGGGCGTTTT 
CAGGTGACAA CTACAGCAAA CAAAGTAGGT CGTTTCTCTG TATCAAGAAC TGAAGACAGG 
CTCACTGAGG CAAAGAAAGA GGGACCGACA GCATCTCCTC CTTTTATGGA TTTGGAACAA 
CCTGTTCTTC CTGCTGCAAT ACCAAAGAAA GAAAAGCCTG AACTGTCAGA GCCTTCACAT 
CTGAATGGGC CATCTTCTGA TTTGGAGGCT GCCTTCCTAA GTAGGGATGT AGATGATGGT 
TCAGGTAGTC CACACTCCCC CCACCAACTG AGCTCAAAGA GCCTACCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAACTC ACTCAACTCC TCTTATATGA GTAGTGACAA TGAGTCAGAT 
ATTGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGACAAACA TCTTAAAGAA 
ATTCAAGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC TAAACTGGGC 
AAGGTTCCTC CTGCTGTCAT TATTCCCCCA GCGGCCCCCC TTTCAGGAAG AAGAAGGCGA 
CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGTATCAGTT CCTTGGGGAG TAAAAGCCCC 
CAGCTTTCAG GTAATATGTC TGGTCAGAGT GAAGCTTCAG TCTTACACCC CCAGCAGACC 
CTCCACCCTC CAGGCAACAT CCCAGAAACT GGGCAGAATC AGTTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTGTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG TCAAGGGACC AGCAGCACAA ACACTGTCGG GGGAACAGTG 
AATAGCCAAG CTGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACGTTC 
ACAGATGACC TGCACAAGTT GGTAGACAAT TGGGCCAGAG ATGCCATGAA TCTTTCAGGC 
AGGAGAGGAA GCAAAGGACA CATGAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTCTCT 
GCACCTGGTC AGCTGTGCAT CTCCATGACC TCAAATCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAT 
GGTTTTCCAC CTACCCCCTT TGGCACTCAG TGGAGTGGGA CAGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACC ACCTCCTTAC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_023535115.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023390883.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

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CloneID	OPt72365
Clone ID Related Accession (Same CDS sequence)	XM_023535115.1
Accession Version	XM_023535115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGCA TGGAATACAG 
CAGACAGCCC CTCCTCAACA GACAGTACAG TATTCACTTC CACAGACATC AACCTCCAGT 
GAGGCCACTA CTGCACAGCC AGTGAGTCAG CCTCAGGCTC CACAGGTCTT GCCTCAAGCA 
TCAGCTGGAA AACAGCTTCC AGTTTCCCAG CCAGTACCAA CTGTCCAAGG CGAACCTCAG 
ATCTCAGTTG CGACACAACC CTCAGTTGTT CCAGTGCATT CTGGTGCTCA TTTCCTCCCT 
ATGGGACAGC CACTTCCTAC TTCCTTACTC CCTCAGTACC CTGTCTCTCA AATTCCTGTA 
TCAACTCCTC ATGTGTCCGC GGCTCAGACA GGTTTCTCAA CCCTTCCCAT CACAATTGCA 
GCTGGCCTTA ATCAGCCTCT GCTCACCTTG GCTTCATCTG CTACAGCAGC CGCAATCCCA 
GGGGGATCAA CTATGGCTCC TAGTCAGCTA CCAGCCCTTC TGCAGCCTGT GACTCAGCTG 
CCAAGTCAGG TTCACCCACA GCTCCTGCAG CCAGCAGTTC AGTCCATGGG AATACCAGCT 
AACCTTGGAC AAGCTGCTGA GGTTCCACTT CCCTCTGGAG ATGTTCTATA CCAGGGCTTC 
CCACCTCGAC TGCCACCACA ATACCCAGGA GATTCAAATA TTGCTCCCTC TTCCAGCGTG 
GCTTCTGTTT GCATTCCTTC TACAGTCTTA TCCCCTCCAA TGCCGACAGA AGCATTAGCT 
ACACCAGGTT ACTTTCCTAC AGTGGTGCAG CCTTATATGG AATCAAATCT TTTGGTTCCT 
GTGGGCAGTG TAGGAGGACA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AGCACAAGCC 
CCCACTGCAT CCTCCCAGCA AGCAGCTCTG GAGAGTACTC AGGGAGTCTC TCAGGTTGCT 
CCTCCAGAGC CAGTTTCGGT AGCACAGCCA CAGCCCACCC AGCCTGCCAC TTTGGTCTCT 
TCTATAGATA GTGCACATTC AGATGTTGCT TCAGGTATGA GCGATGGCAA TGAGAATGTG 
CCATCATCTG GTGGAAGGCA TGAAGGGAGA ACTGCAAAAC GGCATTATCG AAAATCTGTA 
AGAAGTCGCT CTCGTCATGA AAAGACTTCA CGCCCCAAAT TGAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGGGTAGT AGAATGTCAG TTAGAGACTC ACAATCGGAA AATGGTTACA 
TTCAAATTTG ATTTAGATGG TGACAATCCC GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATAGA GAGAGAGTCG TTTGTGGATC AAGTACGGGA AATTATTGAA 
AAAGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CCGAGGGTGA CCAGGGATTG 
GAGAGTCTAC AGGGAAAGGA TGACTATGGC TTTTCAGGTT CTCAGAAATT GGAAGGAGAG 
TTCAAACAAC CACTTCCTGC ATCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT ACCAGAAAGC 
CGATTACGAG AATCAAAAAT TTTCACCAGT GAAATATCAG ATACGGTTGC CGCCTCTACA 
TCTCATGGCC CTGGGGTGAA CTTGTCCCAC TCTGCTTCTT CCCTTAGTCT ACAGCAGGCC 
TTTTCTGAAC TTAGACATGC CCAAGTTGCA GAGGGACCCA ATACAGCACC TCCCAACTTC 
AGTCATATAG GACCAACATT TCCAGTAGTA CCTCCTTCCA TGAGTAGCAT TGCTGGAATC 
CCAACGACAG CAGCAGCCGT ACCTTCAATA TCAGTCCCTG TAACTAGCTG CCCTCTTAAT 
GACATTTCCA CATCAATAAT TCAATCTGAG ATTACAGTGC CCACTGAAAA AGGAGTTGCT 
GGAGTTGCCA CCTGCACAGG TGTAATAACT TCAAGTGGTT TCCCAGTACC ACCTGCGTCT 
GAATCACCAA TTCTTTCCAG TGTGGTTTCA AGTATCACAA TACCAACAGT TGTCTCAGTA 
TCAACAACAT CCCAGTCAGT TCAGGCTCCC ACATCTGGGA GCGTTGTTTC TAGTATAGGC 
ACTTTCCCTT CTATACCAGT TTCAGCAACT TCAGCCTCTG CAGTGGGCAG TGCTGCTGCC 
CCAGGGGCTA AGTCTCCACC TTTGTTATCT GAGCAGTCAG CAGGCATTAC TACAGGGATA 
GCCACATTAA CATCAGTTTC TACCACCACT CCATTCCCAA GCACAGCTTC ACAGCTATCT 
CTTCAGCTTA GCAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAGTGGT GGTTAGTGCA 
CACTCACTAG ATAAAACATC TCATAGCAGT ACAACTGGAC TGGCTTTGTC CCACTCTGCA 
TCATCTTCCA CTTCTTCCCC TGGAGCAGGA GTATCTAGTT CTATTTCTCA ACCTGGTGGA 
TTACATCCTT TGGTCATCCC ATCAGCTGTA GTTTCTACTC CTGTCCTTTC CCAAGCATCA 
GGACCTATTT CTACCCCTTT ATTACCCCAA GGACCTAGCA TCCCACCATT GGTGCAACCT 
GTTGCCAATG TGCCTGCTGT ACAGCAGACA CTAATTCATA GTCAGCCTCA ACCAGCTTTG 
CTTCTCAACC AGCCCCATAC GCACTGCCCT GAAATAGATG CTGATACACA ACCCAAAGCT 
CCTGGGATTG ATGACATAAA GACTCTAGAG GAAAAGTTGC GGTCTCTCTT TAGTGAACAC 
AGCTCATCTG GCGCTCAACA TGCCTCTGTT GCGCTGGAGA CATCACTAGT AGTAGAGACC 
AGTGTCACAC CAGGCATCCC AACCACTGCT GTTGCACCCA GCAAACTCAT GACTTCAACT 
ACAAGTACTT GCTTACCACC AACCAGTTTG CCATTAGGAA CAGCTGGTTT GTCCATTACA 
CCACTGGTCA CAACTGGGCA AGTTTCTACC CCAGTCAGCA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CATCAAAGCT ACCTTTAACT AAGGCTCCAG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCTG GTACTCCATC CAGTGAGCAG CTGCCACCTA TTCCAGGACC TTCACTAACT 
CAGTCCCAGC AACCTCTGGA AGATCTTGAT GCTCAGTTAA GAAGAGCACT TAGTCCAGAG 
GCTATTGCGG TGACATCCGC AGTTGGTCCT GTGTCCGTGG TGGCTCCAGC GGCAGTTACA 
GAAGCAGGAG CACAGCCTCA GAAGGATGTT TCTCAAGTCA CAGAAGGTTC TGTCCCAGCA 
GCTAGTTCAG GAACTGGTGT TTTTAAGACG GGGCGATTTC AGGTTTCAGT TGCAATAGAT 
GATTCCCAGA AAGAGGGTAA AACTAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACTTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCCT GGTGAAGCCA 
GACTCTAATG GGGTAACCAT CCATGGCATC TCTTCAGATA TGCCAGATAG TGCCCACAAA 
ACTCCTGCCG CAGAAGCAAA GTCAGAAACT GGGCAACCTA CCAAGGTTGG GCGTTTTCAG 
GTGACAACTA CAGCAAACAA AGTAGGTCGT TTCTCTGTAT CAAGAACTGA AGACAGGCTC 
ACTGAGGCAA AGAAAGAGGG ACCGACAGCA TCTCCTCCTT TTATGGATTT GGAACAACCT 
GTTCTTCCTG CTGCAATACC AAAGAAAGAA AAGCCTGAAC TGTCAGAGCC TTCACATCTG 
AATGGGCCAT CTTCTGATTT GGAGGCTGCC TTCCTAAGTA GGGATGTAGA TGATGGTTCA 
GGTAGTCCAC ACTCCCCCCA CCAACTGAGC TCAAAGAGCC TACCTATCCA GAATCTAAGT 
CAAAGCCTTA GTAACTCACT CAACTCCTCT TATATGAGTA GTGACAATGA GTCAGATATT 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ACAAACATCT TAAAGAAATT 
CAAGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACTAA ACTGGGCAAG 
GTTCCTCCTG CTGTCATTAT TCCCCCAGCG GCCCCCCTTT CAGGAAGAAG AAGGCGACCC 
ACTAAAAGCA AAGGCAGCAA ATCTAGTCGT ATCAGTTCCT TGGGGAGTAA AAGCCCCCAG 
CTTTCAGGTA ATATGTCTGG TCAGAGTGAA GCTTCAGTCT TACACCCCCA GCAGACCCTC 
CACCCTCCAG GCAACATCCC AGAAACTGGG CAGAATCAGT TGTTACAGCC CCTTAAGCCA 
TCTCCCTCCA GTGACAACCT GTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGTCA AGGGACCAGC AGCACAAACA CTGTCGGGGG AACAGTGAAT 
AGCCAAGCTG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACGTTCACA 
GATGACCTGC ACAAGTTGGT AGACAATTGG GCCAGAGATG CCATGAATCT TTCAGGCAGG 
AGAGGAAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA 
CCTGGTCAGC TGTGCATCTC CATGACCTCA AATCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGT 
TTTCCACCTA CCCCCTTTGG CACTCAGTGG AGTGGGACAG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACCACC TCCTTACAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGTTCC AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023390883.1
CDS	669..7736
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPVSQIPVSTPHVSAAQTGFSTLPITIAAGLNQPLLTLASSATAAAIPGGSTMAPSQLPALLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIAPSSSVASVCIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLLVPVGSVGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPEAIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_023535115.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023535115.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGCA TGGAATACAG 
CAGACAGCCC CTCCTCAACA GACAGTACAG TATTCACTTC CACAGACATC AACCTCCAGT 
GAGGCCACTA CTGCACAGCC AGTGAGTCAG CCTCAGGCTC CACAGGTCTT GCCTCAAGCA 
TCAGCTGGAA AACAGCTTCC AGTTTCCCAG CCAGTACCAA CTGTCCAAGG CGAACCTCAG 
ATCTCAGTTG CGACACAACC CTCAGTTGTT CCAGTGCATT CTGGTGCTCA TTTCCTCCCT 
ATGGGACAGC CACTTCCTAC TTCCTTACTC CCTCAGTACC CTGTCTCTCA AATTCCTGTA 
TCAACTCCTC ATGTGTCCGC GGCTCAGACA GGTTTCTCAA CCCTTCCCAT CACAATTGCA 
GCTGGCCTTA ATCAGCCTCT GCTCACCTTG GCTTCATCTG CTACAGCAGC CGCAATCCCA 
GGGGGATCAA CTATGGCTCC TAGTCAGCTA CCAGCCCTTC TGCAGCCTGT GACTCAGCTG 
CCAAGTCAGG TTCACCCACA GCTCCTGCAG CCAGCAGTTC AGTCCATGGG AATACCAGCT 
AACCTTGGAC AAGCTGCTGA GGTTCCACTT CCCTCTGGAG ATGTTCTATA CCAGGGCTTC 
CCACCTCGAC TGCCACCACA ATACCCAGGA GATTCAAATA TTGCTCCCTC TTCCAGCGTG 
GCTTCTGTTT GCATTCCTTC TACAGTCTTA TCCCCTCCAA TGCCGACAGA AGCATTAGCT 
ACACCAGGTT ACTTTCCTAC AGTGGTGCAG CCTTATATGG AATCAAATCT TTTGGTTCCT 
GTGGGCAGTG TAGGAGGACA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AGCACAAGCC 
CCCACTGCAT CCTCCCAGCA AGCAGCTCTG GAGAGTACTC AGGGAGTCTC TCAGGTTGCT 
CCTCCAGAGC CAGTTTCGGT AGCACAGCCA CAGCCCACCC AGCCTGCCAC TTTGGTCTCT 
TCTATAGATA GTGCACATTC AGATGTTGCT TCAGGTATGA GCGATGGCAA TGAGAATGTG 
CCATCATCTG GTGGAAGGCA TGAAGGGAGA ACTGCAAAAC GGCATTATCG AAAATCTGTA 
AGAAGTCGCT CTCGTCATGA AAAGACTTCA CGCCCCAAAT TGAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGGGTAGT AGAATGTCAG TTAGAGACTC ACAATCGGAA AATGGTTACA 
TTCAAATTTG ATTTAGATGG TGACAATCCC GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATAGA GAGAGAGTCG TTTGTGGATC AAGTACGGGA AATTATTGAA 
AAAGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CCGAGGGTGA CCAGGGATTG 
GAGAGTCTAC AGGGAAAGGA TGACTATGGC TTTTCAGGTT CTCAGAAATT GGAAGGAGAG 
TTCAAACAAC CACTTCCTGC ATCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT ACCAGAAAGC 
CGATTACGAG AATCAAAAAT TTTCACCAGT GAAATATCAG ATACGGTTGC CGCCTCTACA 
TCTCATGGCC CTGGGGTGAA CTTGTCCCAC TCTGCTTCTT CCCTTAGTCT ACAGCAGGCC 
TTTTCTGAAC TTAGACATGC CCAAGTTGCA GAGGGACCCA ATACAGCACC TCCCAACTTC 
AGTCATATAG GACCAACATT TCCAGTAGTA CCTCCTTCCA TGAGTAGCAT TGCTGGAATC 
CCAACGACAG CAGCAGCCGT ACCTTCAATA TCAGTCCCTG TAACTAGCTG CCCTCTTAAT 
GACATTTCCA CATCAATAAT TCAATCTGAG ATTACAGTGC CCACTGAAAA AGGAGTTGCT 
GGAGTTGCCA CCTGCACAGG TGTAATAACT TCAAGTGGTT TCCCAGTACC ACCTGCGTCT 
GAATCACCAA TTCTTTCCAG TGTGGTTTCA AGTATCACAA TACCAACAGT TGTCTCAGTA 
TCAACAACAT CCCAGTCAGT TCAGGCTCCC ACATCTGGGA GCGTTGTTTC TAGTATAGGC 
ACTTTCCCTT CTATACCAGT TTCAGCAACT TCAGCCTCTG CAGTGGGCAG TGCTGCTGCC 
CCAGGGGCTA AGTCTCCACC TTTGTTATCT GAGCAGTCAG CAGGCATTAC TACAGGGATA 
GCCACATTAA CATCAGTTTC TACCACCACT CCATTCCCAA GCACAGCTTC ACAGCTATCT 
CTTCAGCTTA GCAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAGTGGT GGTTAGTGCA 
CACTCACTAG ATAAAACATC TCATAGCAGT ACAACTGGAC TGGCTTTGTC CCACTCTGCA 
TCATCTTCCA CTTCTTCCCC TGGAGCAGGA GTATCTAGTT CTATTTCTCA ACCTGGTGGA 
TTACATCCTT TGGTCATCCC ATCAGCTGTA GTTTCTACTC CTGTCCTTTC CCAAGCATCA 
GGACCTATTT CTACCCCTTT ATTACCCCAA GGACCTAGCA TCCCACCATT GGTGCAACCT 
GTTGCCAATG TGCCTGCTGT ACAGCAGACA CTAATTCATA GTCAGCCTCA ACCAGCTTTG 
CTTCTCAACC AGCCCCATAC GCACTGCCCT GAAATAGATG CTGATACACA ACCCAAAGCT 
CCTGGGATTG ATGACATAAA GACTCTAGAG GAAAAGTTGC GGTCTCTCTT TAGTGAACAC 
AGCTCATCTG GCGCTCAACA TGCCTCTGTT GCGCTGGAGA CATCACTAGT AGTAGAGACC 
AGTGTCACAC CAGGCATCCC AACCACTGCT GTTGCACCCA GCAAACTCAT GACTTCAACT 
ACAAGTACTT GCTTACCACC AACCAGTTTG CCATTAGGAA CAGCTGGTTT GTCCATTACA 
CCACTGGTCA CAACTGGGCA AGTTTCTACC CCAGTCAGCA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CATCAAAGCT ACCTTTAACT AAGGCTCCAG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCTG GTACTCCATC CAGTGAGCAG CTGCCACCTA TTCCAGGACC TTCACTAACT 
CAGTCCCAGC AACCTCTGGA AGATCTTGAT GCTCAGTTAA GAAGAGCACT TAGTCCAGAG 
GCTATTGCGG TGACATCCGC AGTTGGTCCT GTGTCCGTGG TGGCTCCAGC GGCAGTTACA 
GAAGCAGGAG CACAGCCTCA GAAGGATGTT TCTCAAGTCA CAGAAGGTTC TGTCCCAGCA 
GCTAGTTCAG GAACTGGTGT TTTTAAGACG GGGCGATTTC AGGTTTCAGT TGCAATAGAT 
GATTCCCAGA AAGAGGGTAA AACTAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACTTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCCT GGTGAAGCCA 
GACTCTAATG GGGTAACCAT CCATGGCATC TCTTCAGATA TGCCAGATAG TGCCCACAAA 
ACTCCTGCCG CAGAAGCAAA GTCAGAAACT GGGCAACCTA CCAAGGTTGG GCGTTTTCAG 
GTGACAACTA CAGCAAACAA AGTAGGTCGT TTCTCTGTAT CAAGAACTGA AGACAGGCTC 
ACTGAGGCAA AGAAAGAGGG ACCGACAGCA TCTCCTCCTT TTATGGATTT GGAACAACCT 
GTTCTTCCTG CTGCAATACC AAAGAAAGAA AAGCCTGAAC TGTCAGAGCC TTCACATCTG 
AATGGGCCAT CTTCTGATTT GGAGGCTGCC TTCCTAAGTA GGGATGTAGA TGATGGTTCA 
GGTAGTCCAC ACTCCCCCCA CCAACTGAGC TCAAAGAGCC TACCTATCCA GAATCTAAGT 
CAAAGCCTTA GTAACTCACT CAACTCCTCT TATATGAGTA GTGACAATGA GTCAGATATT 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ACAAACATCT TAAAGAAATT 
CAAGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACTAA ACTGGGCAAG 
GTTCCTCCTG CTGTCATTAT TCCCCCAGCG GCCCCCCTTT CAGGAAGAAG AAGGCGACCC 
ACTAAAAGCA AAGGCAGCAA ATCTAGTCGT ATCAGTTCCT TGGGGAGTAA AAGCCCCCAG 
CTTTCAGGTA ATATGTCTGG TCAGAGTGAA GCTTCAGTCT TACACCCCCA GCAGACCCTC 
CACCCTCCAG GCAACATCCC AGAAACTGGG CAGAATCAGT TGTTACAGCC CCTTAAGCCA 
TCTCCCTCCA GTGACAACCT GTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGTCA AGGGACCAGC AGCACAAACA CTGTCGGGGG AACAGTGAAT 
AGCCAAGCTG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACGTTCACA 
GATGACCTGC ACAAGTTGGT AGACAATTGG GCCAGAGATG CCATGAATCT TTCAGGCAGG 
AGAGGAAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA 
CCTGGTCAGC TGTGCATCTC CATGACCTCA AATCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGT 
TTTCCACCTA CCCCCTTTGG CACTCAGTGG AGTGGGACAG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACCACC TCCTTACAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGTTCC AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_023535116.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023390884.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

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CloneID	OPt72366
Clone ID Related Accession (Same CDS sequence)	XM_023535116.1
Accession Version	XM_023535116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG TCTCTCAAAT TCCTGTATCA ACTCCTCATG TGTCCGCGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCCTTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCCGC AATCCCAGGG GGATCAACTA TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT CCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GAGTACTCAG GGAGTCTCTC AGGTTGCTCC TCCAGAGCCA 
GTTTCGGTAG CACAGCCACA GCCCACCCAG CCTGCCACTT TGGTCTCTTC TATAGATAGT 
GCACATTCAG ATGTTGCTTC AGGTATGAGC GATGGCAATG AGAATGTGCC ATCATCTGGT 
GGAAGGCATG AAGGGAGAAC TGCAAAACGG CATTATCGAA AATCTGTAAG AAGTCGCTCT 
CGTCATGAAA AGACTTCACG CCCCAAATTG AGAATTTTGA ATGTTTCAAA TAAAGGAGAC 
CGGGTAGTAG AATGTCAGTT AGAGACTCAC AATCGGAAAA TGGTTACATT CAAATTTGAT 
TTAGATGGTG ACAATCCCGA GGAGATAGCA ACAATTATGG TGAACAATGA CTTTATTCTA 
GCAATAGAGA GAGAGTCGTT TGTGGATCAA GTACGGGAAA TTATTGAAAA AGCTGATGAA 
ATGCTCAGTG AGGATGTCAG TGTGGAACCC GAGGGTGACC AGGGATTGGA GAGTCTACAG 
GGAAAGGATG ACTATGGCTT TTCAGGTTCT CAGAAATTGG AAGGAGAGTT CAAACAACCA 
CTTCCTGCAT CTTCCATGCC ACAGCAAATA GGCATTCCTA CCAGTTCTTT AACTCAAGTT 
GTTCATTCTG CTGGAAGACG GTTTATAGTG AGTCCTGTAC CAGAAAGCCG ATTACGAGAA 
TCAAAAATTT TCACCAGTGA AATATCAGAT ACGGTTGCCG CCTCTACATC TCATGGCCCT 
GGGGTGAACT TGTCCCACTC TGCTTCTTCC CTTAGTCTAC AGCAGGCCTT TTCTGAACTT 
AGACATGCCC AAGTTGCAGA GGGACCCAAT ACAGCACCTC CCAACTTCAG TCATATAGGA 
CCAACATTTC CAGTAGTACC TCCTTCCATG AGTAGCATTG CTGGAATCCC AACGACAGCA 
GCAGCCGTAC CTTCAATATC AGTCCCTGTA ACTAGCTGCC CTCTTAATGA CATTTCCACA 
TCAATAATTC AATCTGAGAT TACAGTGCCC ACTGAAAAAG GAGTTGCTGG AGTTGCCACC 
TGCACAGGTG TAATAACTTC AAGTGGTTTC CCAGTACCAC CTGCGTCTGA ATCACCAATT 
CTTTCCAGTG TGGTTTCAAG TATCACAATA CCAACAGTTG TCTCAGTATC AACAACATCC 
CAGTCAGTTC AGGCTCCCAC ATCTGGGAGC GTTGTTTCTA GTATAGGCAC TTTCCCTTCT 
ATACCAGTTT CAGCAACTTC AGCCTCTGCA GTGGGCAGTG CTGCTGCCCC AGGGGCTAAG 
TCTCCACCTT TGTTATCTGA GCAGTCAGCA GGCATTACTA CAGGGATAGC CACATTAACA 
TCAGTTTCTA CCACCACTCC ATTCCCAAGC ACAGCTTCAC AGCTATCTCT TCAGCTTAGC 
AGCAGCACCT CTGCTCCTAC TCTAGCTGAA ACAGTGGTGG TTAGTGCACA CTCACTAGAT 
AAAACATCTC ATAGCAGTAC AACTGGACTG GCTTTGTCCC ACTCTGCATC ATCTTCCACT 
TCTTCCCCTG GAGCAGGAGT ATCTAGTTCT ATTTCTCAAC CTGGTGGATT ACATCCTTTG 
GTCATCCCAT CAGCTGTAGT TTCTACTCCT GTCCTTTCCC AAGCATCAGG ACCTATTTCT 
ACCCCTTTAT TACCCCAAGG ACCTAGCATC CCACCATTGG TGCAACCTGT TGCCAATGTG 
CCTGCTGTAC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTGCT TCTCAACCAG 
CCCCATACGC ACTGCCCTGA AATAGATGCT GATACACAAC CCAAAGCTCC TGGGATTGAT 
GACATAAAGA CTCTAGAGGA AAAGTTGCGG TCTCTCTTTA GTGAACACAG CTCATCTGGC 
GCTCAACATG CCTCTGTTGC GCTGGAGACA TCACTAGTAG TAGAGACCAG TGTCACACCA 
GGCATCCCAA CCACTGCTGT TGCACCCAGC AAACTCATGA CTTCAACTAC AAGTACTTGC 
TTACCACCAA CCAGTTTGCC ATTAGGAACA GCTGGTTTGT CCATTACACC ACTGGTCACA 
ACTGGGCAAG TTTCTACCCC AGTCAGCACT ACATCAGGAG TGAAACCTGG AACTGCTCCA 
TCAAAGCTAC CTTTAACTAA GGCTCCAGTG CTGCCAGTGG GTACTGAACT TCCAGCTGGT 
ACTCCATCCA GTGAGCAGCT GCCACCTATT CCAGGACCTT CACTAACTCA GTCCCAGCAA 
CCTCTGGAAG ATCTTGATGC TCAGTTAAGA AGAGCACTTA GTCCAGAGGC TATTGCGGTG 
ACATCCGCAG TTGGTCCTGT GTCCGTGGTG GCTCCAGCGG CAGTTACAGA AGCAGGAGCA 
CAGCCTCAGA AGGATGTTTC TCAAGTCACA GAAGGTTCTG TCCCAGCAGC TAGTTCAGGA 
ACTGGTGTTT TTAAGACGGG GCGATTTCAG GTTTCAGTTG CAATAGATGA TTCCCAGAAA 
GAGGGTAAAA CTAAGTCAGA AGATGCAAAG TCTGTTCATT TTGAATCCAG CACTTCAGAG 
TCCTCAGTGC TATCAAGTAG TAGTCCAGAG AGTACCCTGG TGAAGCCAGA CTCTAATGGG 
GTAACCATCC ATGGCATCTC TTCAGATATG CCAGATAGTG CCCACAAAAC TCCTGCCGCA 
GAAGCAAAGT CAGAAACTGG GCAACCTACC AAGGTTGGGC GTTTTCAGGT GACAACTACA 
GCAAACAAAG TAGGTCGTTT CTCTGTATCA AGAACTGAAG ACAGGCTCAC TGAGGCAAAG 
AAAGAGGGAC CGACAGCATC TCCTCCTTTT ATGGATTTGG AACAACCTGT TCTTCCTGCT 
GCAATACCAA AGAAAGAAAA GCCTGAACTG TCAGAGCCTT CACATCTGAA TGGGCCATCT 
TCTGATTTGG AGGCTGCCTT CCTAAGTAGG GATGTAGATG ATGGTTCAGG TAGTCCACAC 
TCCCCCCACC AACTGAGCTC AAAGAGCCTA CCTATCCAGA ATCTAAGTCA AAGCCTTAGT 
AACTCACTCA ACTCCTCTTA TATGAGTAGT GACAATGAGT CAGATATTGA AGATGAAGAC 
TTAAAGTTAG AGCTGCGACG ACTACGAGAC AAACATCTTA AAGAAATTCA AGACCTGCAG 
AGTCGCCAGA AGCATGAAAT TGAATCTTTG TATACTAAAC TGGGCAAGGT TCCTCCTGCT 
GTCATTATTC CCCCAGCGGC CCCCCTTTCA GGAAGAAGAA GGCGACCCAC TAAAAGCAAA 
GGCAGCAAAT CTAGTCGTAT CAGTTCCTTG GGGAGTAAAA GCCCCCAGCT TTCAGGTAAT 
ATGTCTGGTC AGAGTGAAGC TTCAGTCTTA CACCCCCAGC AGACCCTCCA CCCTCCAGGC 
AACATCCCAG AAACTGGGCA GAATCAGTTG TTACAGCCCC TTAAGCCATC TCCCTCCAGT 
GACAACCTGT ATTCAGCCTT CACCAGTGAT GGTGCCATTT CAGTACCAAG CCTTTCTGCT 
CCAGGTCAAG GGACCAGCAG CACAAACACT GTCGGGGGAA CAGTGAATAG CCAAGCTGCC 
CAAGCTCAGC CTCCTGCCAT GACGTCCAGC AGGAAGGGCA CGTTCACAGA TGACCTGCAC 
AAGTTGGTAG ACAATTGGGC CAGAGATGCC ATGAATCTTT CAGGCAGGAG AGGAAGCAAA 
GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGGAAGT TCTCTGCACC TGGTCAGCTG 
TGCATCTCCA TGACCTCAAA TCTGGGTGGC TCTGCCCCCA TCTCTGCAGC ATCAGCTACC 
TCTCTAGGTC ACTTCACCAA GTCTATGTGC CCCCCACAGC AGTATGGTTT TCCACCTACC 
CCCTTTGGCA CTCAGTGGAG TGGGACAGGT GGCCCAGCAC CACAGCCACT TGGCCAGTTC 
CAACCTGTGG GAACCACCTC CTTACAGAAT TTCAACATCA GCAATTTGCA GAAATCCATC 
AGCAACCCCC CAGGTTCCAA CCTGCGGACC ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023390884.1
CDS	665..7540
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHPQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPVSQIPVSTPHVSAAQTGFSTLPITIAAGLNQPLLTLASSATAAAIPGGSTMAPSQLPALLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQSTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPEAIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_023535116.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023535116.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG TCTCTCAAAT TCCTGTATCA ACTCCTCATG TGTCCGCGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCCTTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCCGC AATCCCAGGG GGATCAACTA TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT CCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GAGTACTCAG GGAGTCTCTC AGGTTGCTCC TCCAGAGCCA 
GTTTCGGTAG CACAGCCACA GCCCACCCAG CCTGCCACTT TGGTCTCTTC TATAGATAGT 
GCACATTCAG ATGTTGCTTC AGGTATGAGC GATGGCAATG AGAATGTGCC ATCATCTGGT 
GGAAGGCATG AAGGGAGAAC TGCAAAACGG CATTATCGAA AATCTGTAAG AAGTCGCTCT 
CGTCATGAAA AGACTTCACG CCCCAAATTG AGAATTTTGA ATGTTTCAAA TAAAGGAGAC 
CGGGTAGTAG AATGTCAGTT AGAGACTCAC AATCGGAAAA TGGTTACATT CAAATTTGAT 
TTAGATGGTG ACAATCCCGA GGAGATAGCA ACAATTATGG TGAACAATGA CTTTATTCTA 
GCAATAGAGA GAGAGTCGTT TGTGGATCAA GTACGGGAAA TTATTGAAAA AGCTGATGAA 
ATGCTCAGTG AGGATGTCAG TGTGGAACCC GAGGGTGACC AGGGATTGGA GAGTCTACAG 
GGAAAGGATG ACTATGGCTT TTCAGGTTCT CAGAAATTGG AAGGAGAGTT CAAACAACCA 
CTTCCTGCAT CTTCCATGCC ACAGCAAATA GGCATTCCTA CCAGTTCTTT AACTCAAGTT 
GTTCATTCTG CTGGAAGACG GTTTATAGTG AGTCCTGTAC CAGAAAGCCG ATTACGAGAA 
TCAAAAATTT TCACCAGTGA AATATCAGAT ACGGTTGCCG CCTCTACATC TCATGGCCCT 
GGGGTGAACT TGTCCCACTC TGCTTCTTCC CTTAGTCTAC AGCAGGCCTT TTCTGAACTT 
AGACATGCCC AAGTTGCAGA GGGACCCAAT ACAGCACCTC CCAACTTCAG TCATATAGGA 
CCAACATTTC CAGTAGTACC TCCTTCCATG AGTAGCATTG CTGGAATCCC AACGACAGCA 
GCAGCCGTAC CTTCAATATC AGTCCCTGTA ACTAGCTGCC CTCTTAATGA CATTTCCACA 
TCAATAATTC AATCTGAGAT TACAGTGCCC ACTGAAAAAG GAGTTGCTGG AGTTGCCACC 
TGCACAGGTG TAATAACTTC AAGTGGTTTC CCAGTACCAC CTGCGTCTGA ATCACCAATT 
CTTTCCAGTG TGGTTTCAAG TATCACAATA CCAACAGTTG TCTCAGTATC AACAACATCC 
CAGTCAGTTC AGGCTCCCAC ATCTGGGAGC GTTGTTTCTA GTATAGGCAC TTTCCCTTCT 
ATACCAGTTT CAGCAACTTC AGCCTCTGCA GTGGGCAGTG CTGCTGCCCC AGGGGCTAAG 
TCTCCACCTT TGTTATCTGA GCAGTCAGCA GGCATTACTA CAGGGATAGC CACATTAACA 
TCAGTTTCTA CCACCACTCC ATTCCCAAGC ACAGCTTCAC AGCTATCTCT TCAGCTTAGC 
AGCAGCACCT CTGCTCCTAC TCTAGCTGAA ACAGTGGTGG TTAGTGCACA CTCACTAGAT 
AAAACATCTC ATAGCAGTAC AACTGGACTG GCTTTGTCCC ACTCTGCATC ATCTTCCACT 
TCTTCCCCTG GAGCAGGAGT ATCTAGTTCT ATTTCTCAAC CTGGTGGATT ACATCCTTTG 
GTCATCCCAT CAGCTGTAGT TTCTACTCCT GTCCTTTCCC AAGCATCAGG ACCTATTTCT 
ACCCCTTTAT TACCCCAAGG ACCTAGCATC CCACCATTGG TGCAACCTGT TGCCAATGTG 
CCTGCTGTAC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTGCT TCTCAACCAG 
CCCCATACGC ACTGCCCTGA AATAGATGCT GATACACAAC CCAAAGCTCC TGGGATTGAT 
GACATAAAGA CTCTAGAGGA AAAGTTGCGG TCTCTCTTTA GTGAACACAG CTCATCTGGC 
GCTCAACATG CCTCTGTTGC GCTGGAGACA TCACTAGTAG TAGAGACCAG TGTCACACCA 
GGCATCCCAA CCACTGCTGT TGCACCCAGC AAACTCATGA CTTCAACTAC AAGTACTTGC 
TTACCACCAA CCAGTTTGCC ATTAGGAACA GCTGGTTTGT CCATTACACC ACTGGTCACA 
ACTGGGCAAG TTTCTACCCC AGTCAGCACT ACATCAGGAG TGAAACCTGG AACTGCTCCA 
TCAAAGCTAC CTTTAACTAA GGCTCCAGTG CTGCCAGTGG GTACTGAACT TCCAGCTGGT 
ACTCCATCCA GTGAGCAGCT GCCACCTATT CCAGGACCTT CACTAACTCA GTCCCAGCAA 
CCTCTGGAAG ATCTTGATGC TCAGTTAAGA AGAGCACTTA GTCCAGAGGC TATTGCGGTG 
ACATCCGCAG TTGGTCCTGT GTCCGTGGTG GCTCCAGCGG CAGTTACAGA AGCAGGAGCA 
CAGCCTCAGA AGGATGTTTC TCAAGTCACA GAAGGTTCTG TCCCAGCAGC TAGTTCAGGA 
ACTGGTGTTT TTAAGACGGG GCGATTTCAG GTTTCAGTTG CAATAGATGA TTCCCAGAAA 
GAGGGTAAAA CTAAGTCAGA AGATGCAAAG TCTGTTCATT TTGAATCCAG CACTTCAGAG 
TCCTCAGTGC TATCAAGTAG TAGTCCAGAG AGTACCCTGG TGAAGCCAGA CTCTAATGGG 
GTAACCATCC ATGGCATCTC TTCAGATATG CCAGATAGTG CCCACAAAAC TCCTGCCGCA 
GAAGCAAAGT CAGAAACTGG GCAACCTACC AAGGTTGGGC GTTTTCAGGT GACAACTACA 
GCAAACAAAG TAGGTCGTTT CTCTGTATCA AGAACTGAAG ACAGGCTCAC TGAGGCAAAG 
AAAGAGGGAC CGACAGCATC TCCTCCTTTT ATGGATTTGG AACAACCTGT TCTTCCTGCT 
GCAATACCAA AGAAAGAAAA GCCTGAACTG TCAGAGCCTT CACATCTGAA TGGGCCATCT 
TCTGATTTGG AGGCTGCCTT CCTAAGTAGG GATGTAGATG ATGGTTCAGG TAGTCCACAC 
TCCCCCCACC AACTGAGCTC AAAGAGCCTA CCTATCCAGA ATCTAAGTCA AAGCCTTAGT 
AACTCACTCA ACTCCTCTTA TATGAGTAGT GACAATGAGT CAGATATTGA AGATGAAGAC 
TTAAAGTTAG AGCTGCGACG ACTACGAGAC AAACATCTTA AAGAAATTCA AGACCTGCAG 
AGTCGCCAGA AGCATGAAAT TGAATCTTTG TATACTAAAC TGGGCAAGGT TCCTCCTGCT 
GTCATTATTC CCCCAGCGGC CCCCCTTTCA GGAAGAAGAA GGCGACCCAC TAAAAGCAAA 
GGCAGCAAAT CTAGTCGTAT CAGTTCCTTG GGGAGTAAAA GCCCCCAGCT TTCAGGTAAT 
ATGTCTGGTC AGAGTGAAGC TTCAGTCTTA CACCCCCAGC AGACCCTCCA CCCTCCAGGC 
AACATCCCAG AAACTGGGCA GAATCAGTTG TTACAGCCCC TTAAGCCATC TCCCTCCAGT 
GACAACCTGT ATTCAGCCTT CACCAGTGAT GGTGCCATTT CAGTACCAAG CCTTTCTGCT 
CCAGGTCAAG GGACCAGCAG CACAAACACT GTCGGGGGAA CAGTGAATAG CCAAGCTGCC 
CAAGCTCAGC CTCCTGCCAT GACGTCCAGC AGGAAGGGCA CGTTCACAGA TGACCTGCAC 
AAGTTGGTAG ACAATTGGGC CAGAGATGCC ATGAATCTTT CAGGCAGGAG AGGAAGCAAA 
GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGGAAGT TCTCTGCACC TGGTCAGCTG 
TGCATCTCCA TGACCTCAAA TCTGGGTGGC TCTGCCCCCA TCTCTGCAGC ATCAGCTACC 
TCTCTAGGTC ACTTCACCAA GTCTATGTGC CCCCCACAGC AGTATGGTTT TCCACCTACC 
CCCTTTGGCA CTCAGTGGAG TGGGACAGGT GGCCCAGCAC CACAGCCACT TGGCCAGTTC 
CAACCTGTGG GAACCACCTC CTTACAGAAT TTCAACATCA GCAATTTGCA GAAATCCATC 
AGCAACCCCC CAGGTTCCAA CCTGCGGACC ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_023535117.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023390885.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

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CloneID	OPt72367
Clone ID Related Accession (Same CDS sequence)	XM_023535117.1
Accession Version	XM_023535117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG TCTCTCAAAT TCCTGTATCA ACTCCTCATG TGTCCGCGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCCTTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCCGC AATCCCAGGG GGATCAACTA TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT CCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTCCCTCTTC CAGCGTGGCT TCTGTTTGCA TTCCTTCTAC AGTCTTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTTT GGTTCCTGTG GGCAGTGTAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCACTTT GGTCTCTTCT ATAGATAGTG CACATTCAGA TGTTGCTTCA 
GGTATGAGCG ATGGCAATGA GAATGTGCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGTCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAGTTA 
GAGACTCACA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCCGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCGTTT 
GTGGATCAAG TACGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCCG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA AGGAGAGTTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GCATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC TGGAAGACGG 
TTTATAGTGA GTCCTGTACC AGAAAGCCGA TTACGAGAAT CAAAAATTTT CACCAGTGAA 
ATATCAGATA CGGTTGCCGC CTCTACATCT CATGGCCCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTTA GACATGCCCA AGTTGCAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATATAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAATCCCA ACGACAGCAG CAGCCGTACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC ATTTCCACAT CAATAATTCA ATCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCACC TGCGTCTGAA TCACCAATTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CAACAGTTGT CTCAGTATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TATAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCAGTGC TGCTGCCCCA GGGGCTAAGT CTCCACCTTT GTTATCTGAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCACTCCA 
TTCCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCACTAGATA AAACATCTCA TAGCAGTACA 
ACTGGACTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAACC TGGTGGATTA CATCCTTTGG TCATCCCATC AGCTGTAGTT 
TCTACTCCTG TCCTTTCCCA AGCATCAGGA CCTATTTCTA CCCCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CTCAACCAGC CCCATACGCA CTGCCCTGAA 
ATAGATGCTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TCTAGAGGAA 
AAGTTGCGGT CTCTCTTTAG TGAACACAGC TCATCTGGCG CTCAACATGC CTCTGTTGCG 
CTGGAGACAT CACTAGTAGT AGAGACCAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CATTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCA 
GTCAGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGGCT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG AAGGTTCTGT CCCAGCAGCT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAGAAAG AGGGTAAAAC TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGAC TCTAATGGGG TAACCATCCA TGGCATCTCT 
TCAGATATGC CAGATAGTGC CCACAAAACT CCTGCCGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGGCG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGCTCACT GAGGCAAAGA AAGAGGGACC GACAGCATCT 
CCTCCTTTTA TGGATTTGGA ACAACCTGTT CTTCCTGCTG CAATACCAAA GAAAGAAAAG 
CCTGAACTGT CAGAGCCTTC ACATCTGAAT GGGCCATCTT CTGATTTGGA GGCTGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CCCCCCACCA ACTGAGCTCA 
AAGAGCCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAT 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAAATTCAA GACCTGCAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTAC ACCCCCAGCA GACCCTCCAC CCTCCAGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG GAAGATGGTG 
AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT AGTAATTATC TTTTCTCTCT GTTACCTTTG 
GACTTGATTG CAAAGCCTGT TATCCATGAG TTCTTTGAAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023390885.1
CDS	671..7435
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHPQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPVSQIPVSTPHVSAAQTGFSTLPITIAAGLNQPLLTLASSATAAAIPGGSTMAPSQLPALLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIAPSSSVASVCIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLLVPVGSVGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPEAIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGKMVKKVCPCNQLCSNYLFSLLPLDLIAKPVIHEFFEP

Target ORF information:

RefSeq Version	XM_023535117.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023535117.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG TCTCTCAAAT TCCTGTATCA ACTCCTCATG TGTCCGCGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCCTTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCCGC AATCCCAGGG GGATCAACTA TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT CCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTCCCTCTTC CAGCGTGGCT TCTGTTTGCA TTCCTTCTAC AGTCTTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTTT GGTTCCTGTG GGCAGTGTAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCACTTT GGTCTCTTCT ATAGATAGTG CACATTCAGA TGTTGCTTCA 
GGTATGAGCG ATGGCAATGA GAATGTGCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGTCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAGTTA 
GAGACTCACA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCCGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCGTTT 
GTGGATCAAG TACGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCCG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA AGGAGAGTTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GCATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC TGGAAGACGG 
TTTATAGTGA GTCCTGTACC AGAAAGCCGA TTACGAGAAT CAAAAATTTT CACCAGTGAA 
ATATCAGATA CGGTTGCCGC CTCTACATCT CATGGCCCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTTA GACATGCCCA AGTTGCAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATATAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAATCCCA ACGACAGCAG CAGCCGTACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC ATTTCCACAT CAATAATTCA ATCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCACC TGCGTCTGAA TCACCAATTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CAACAGTTGT CTCAGTATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TATAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCAGTGC TGCTGCCCCA GGGGCTAAGT CTCCACCTTT GTTATCTGAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCACTCCA 
TTCCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCACTAGATA AAACATCTCA TAGCAGTACA 
ACTGGACTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAACC TGGTGGATTA CATCCTTTGG TCATCCCATC AGCTGTAGTT 
TCTACTCCTG TCCTTTCCCA AGCATCAGGA CCTATTTCTA CCCCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CTCAACCAGC CCCATACGCA CTGCCCTGAA 
ATAGATGCTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TCTAGAGGAA 
AAGTTGCGGT CTCTCTTTAG TGAACACAGC TCATCTGGCG CTCAACATGC CTCTGTTGCG 
CTGGAGACAT CACTAGTAGT AGAGACCAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CATTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCA 
GTCAGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGGCT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG AAGGTTCTGT CCCAGCAGCT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAGAAAG AGGGTAAAAC TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGAC TCTAATGGGG TAACCATCCA TGGCATCTCT 
TCAGATATGC CAGATAGTGC CCACAAAACT CCTGCCGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGGCG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGCTCACT GAGGCAAAGA AAGAGGGACC GACAGCATCT 
CCTCCTTTTA TGGATTTGGA ACAACCTGTT CTTCCTGCTG CAATACCAAA GAAAGAAAAG 
CCTGAACTGT CAGAGCCTTC ACATCTGAAT GGGCCATCTT CTGATTTGGA GGCTGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CCCCCCACCA ACTGAGCTCA 
AAGAGCCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAT 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAAATTCAA GACCTGCAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTAC ACCCCCAGCA GACCCTCCAC CCTCCAGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG GAAGATGGTG 
AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT AGTAATTATC TTTTCTCTCT GTTACCTTTG 
GACTTGATTG CAAAGCCTGT TATCCATGAG TTCTTTGAAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_023535118.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023390886.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

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CloneID	OPt72368
Clone ID Related Accession (Same CDS sequence)	XM_023535118.1
Accession Version	XM_023535118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCACT 
TTGGTCTCTT CTATAGATAG TGCACATTCA GATGTTGCTT CAGGTATGAG CGATGGCAAT 
GAGAATGTGC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGTCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAGT TAGAGACTCA CAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCCG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCGT TTGTGGATCA AGTACGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC CGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAAGGAGAGT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGCATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCTGGAAGAC GGTTTATAGT GAGTCCTGTA 
CCAGAAAGCC GATTACGAGA ATCAAAAATT TTCACCAGTG AAATATCAGA TACGGTTGCC 
GCCTCTACAT CTCATGGCCC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT TAGACATGCC CAAGTTGCAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATATAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAATCC CAACGACAGC AGCAGCCGTA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACATTTCCAC ATCAATAATT CAATCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCA 
CCTGCGTCTG AATCACCAAT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCAACAGTT 
GTCTCAGTAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTATAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCAGT 
GCTGCTGCCC CAGGGGCTAA GTCTCCACCT TTGTTATCTG AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCACTC CATTCCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCACTAGA TAAAACATCT CATAGCAGTA CAACTGGACT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAA 
CCTGGTGGAT TACATCCTTT GGTCATCCCA TCAGCTGTAG TTTCTACTCC TGTCCTTTCC 
CAAGCATCAG GACCTATTTC TACCCCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCTCAACCA GCCCCATACG CACTGCCCTG AAATAGATGC TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTCTAGAGG AAAAGTTGCG GTCTCTCTTT 
AGTGAACACA GCTCATCTGG CGCTCAACAT GCCTCTGTTG CGCTGGAGAC ATCACTAGTA 
GTAGAGACCA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCATTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CAGTCAGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGTCCCAGCA ACCTCTGGAA GATCTTGATG CTCAGTTAAG AAGAGCACTT 
AGTCCAGAGG CTATTGCGGT GACATCCGCA GTTGGTCCTG TGTCCGTGGT GGCTCCAGCG 
GCAGTTACAG AAGCAGGAGC ACAGCCTCAG AAGGATGTTT CTCAAGTCAC AGAAGGTTCT 
GTCCCAGCAG CTAGTTCAGG AACTGGTGTT TTTAAGACGG GGCGATTTCA GGTTTCAGTT 
GCAATAGATG ATTCCCAGAA AGAGGGTAAA ACTAAGTCAG AAGATGCAAA GTCTGTTCAT 
TTTGAATCCA GCACTTCAGA GTCCTCAGTG CTATCAAGTA GTAGTCCAGA GAGTACCCTG 
GTGAAGCCAG ACTCTAATGG GGTAACCATC CATGGCATCT CTTCAGATAT GCCAGATAGT 
GCCCACAAAA CTCCTGCCGC AGAAGCAAAG TCAGAAACTG GGCAACCTAC CAAGGTTGGG 
CGTTTTCAGG TGACAACTAC AGCAAACAAA GTAGGTCGTT TCTCTGTATC AAGAACTGAA 
GACAGGCTCA CTGAGGCAAA GAAAGAGGGA CCGACAGCAT CTCCTCCTTT TATGGATTTG 
GAACAACCTG TTCTTCCTGC TGCAATACCA AAGAAAGAAA AGCCTGAACT GTCAGAGCCT 
TCACATCTGA ATGGGCCATC TTCTGATTTG GAGGCTGCCT TCCTAAGTAG GGATGTAGAT 
GATGGTTCAG GTAGTCCACA CTCCCCCCAC CAACTGAGCT CAAAGAGCCT ACCTATCCAG 
AATCTAAGTC AAAGCCTTAG TAACTCACTC AACTCCTCTT ATATGAGTAG TGACAATGAG 
TCAGATATTG AAGATGAAGA CTTAAAGTTA GAGCTGCGAC GACTACGAGA CAAACATCTT 
AAAGAAATTC AAGACCTGCA GAGTCGCCAG AAGCATGAAA TTGAATCTTT GTATACTAAA 
CTGGGCAAGG TTCCTCCTGC TGTCATTATT CCCCCAGCGG CCCCCCTTTC AGGAAGAAGA 
AGGCGACCCA CTAAAAGCAA AGGCAGCAAA TCTAGTCGTA TCAGTTCCTT GGGGAGTAAA 
AGCCCCCAGC TTTCAGGTAA TATGTCTGGT CAGAGTGAAG CTTCAGTCTT ACACCCCCAG 
CAGACCCTCC ACCCTCCAGG CAACATCCCA GAAACTGGGC AGAATCAGTT GTTACAGCCC 
CTTAAGCCAT CTCCCTCCAG TGACAACCTG TATTCAGCCT TCACCAGTGA TGGTGCCATT 
TCAGTACCAA GCCTTTCTGC TCCAGGTCAA GGGACCAGCA GCACAAACAC TGTCGGGGGA 
ACAGTGAATA GCCAAGCTGC CCAAGCTCAG CCTCCTGCCA TGACGTCCAG CAGGAAGGGC 
ACGTTCACAG ATGACCTGCA CAAGTTGGTA GACAATTGGG CCAGAGATGC CATGAATCTT 
TCAGGCAGGA GAGGAAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGGAAG 
TTCTCTGCAC CTGGTCAGCT GTGCATCTCC ATGACCTCAA ATCTGGGTGG CTCTGCCCCC 
ATCTCTGCAG CATCAGCTAC CTCTCTAGGT CACTTCACCA AGTCTATGTG CCCCCCACAG 
CAGTATGGTT TTCCACCTAC CCCCTTTGGC ACTCAGTGGA GTGGGACAGG TGGCCCAGCA 
CCACAGCCAC TTGGCCAGTT CCAACCTGTG GGAACCACCT CCTTACAGAA TTTCAACATC 
AGCAATTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023390886.1
CDS	662..7078
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHPQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQSTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPEAIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_023535118.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023535118.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AACCATCCTC AAGTAGTGTA ACAGGAGTTT CATCTTCCCA ATCTATACAA 
CATCCTCAGC AGCAGCATGG AATACAGCAG ACAGCCCCTC CTCAACAGAC AGTACAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAGGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCACT 
TTGGTCTCTT CTATAGATAG TGCACATTCA GATGTTGCTT CAGGTATGAG CGATGGCAAT 
GAGAATGTGC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGTCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAGT TAGAGACTCA CAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCCG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCGT TTGTGGATCA AGTACGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC CGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAAGGAGAGT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGCATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCTGGAAGAC GGTTTATAGT GAGTCCTGTA 
CCAGAAAGCC GATTACGAGA ATCAAAAATT TTCACCAGTG AAATATCAGA TACGGTTGCC 
GCCTCTACAT CTCATGGCCC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT TAGACATGCC CAAGTTGCAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATATAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAATCC CAACGACAGC AGCAGCCGTA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACATTTCCAC ATCAATAATT CAATCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCA 
CCTGCGTCTG AATCACCAAT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCAACAGTT 
GTCTCAGTAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTATAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCAGT 
GCTGCTGCCC CAGGGGCTAA GTCTCCACCT TTGTTATCTG AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCACTC CATTCCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCACTAGA TAAAACATCT CATAGCAGTA CAACTGGACT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAA 
CCTGGTGGAT TACATCCTTT GGTCATCCCA TCAGCTGTAG TTTCTACTCC TGTCCTTTCC 
CAAGCATCAG GACCTATTTC TACCCCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCTCAACCA GCCCCATACG CACTGCCCTG AAATAGATGC TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTCTAGAGG AAAAGTTGCG GTCTCTCTTT 
AGTGAACACA GCTCATCTGG CGCTCAACAT GCCTCTGTTG CGCTGGAGAC ATCACTAGTA 
GTAGAGACCA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCATTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CAGTCAGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGTCCCAGCA ACCTCTGGAA GATCTTGATG CTCAGTTAAG AAGAGCACTT 
AGTCCAGAGG CTATTGCGGT GACATCCGCA GTTGGTCCTG TGTCCGTGGT GGCTCCAGCG 
GCAGTTACAG AAGCAGGAGC ACAGCCTCAG AAGGATGTTT CTCAAGTCAC AGAAGGTTCT 
GTCCCAGCAG CTAGTTCAGG AACTGGTGTT TTTAAGACGG GGCGATTTCA GGTTTCAGTT 
GCAATAGATG ATTCCCAGAA AGAGGGTAAA ACTAAGTCAG AAGATGCAAA GTCTGTTCAT 
TTTGAATCCA GCACTTCAGA GTCCTCAGTG CTATCAAGTA GTAGTCCAGA GAGTACCCTG 
GTGAAGCCAG ACTCTAATGG GGTAACCATC CATGGCATCT CTTCAGATAT GCCAGATAGT 
GCCCACAAAA CTCCTGCCGC AGAAGCAAAG TCAGAAACTG GGCAACCTAC CAAGGTTGGG 
CGTTTTCAGG TGACAACTAC AGCAAACAAA GTAGGTCGTT TCTCTGTATC AAGAACTGAA 
GACAGGCTCA CTGAGGCAAA GAAAGAGGGA CCGACAGCAT CTCCTCCTTT TATGGATTTG 
GAACAACCTG TTCTTCCTGC TGCAATACCA AAGAAAGAAA AGCCTGAACT GTCAGAGCCT 
TCACATCTGA ATGGGCCATC TTCTGATTTG GAGGCTGCCT TCCTAAGTAG GGATGTAGAT 
GATGGTTCAG GTAGTCCACA CTCCCCCCAC CAACTGAGCT CAAAGAGCCT ACCTATCCAG 
AATCTAAGTC AAAGCCTTAG TAACTCACTC AACTCCTCTT ATATGAGTAG TGACAATGAG 
TCAGATATTG AAGATGAAGA CTTAAAGTTA GAGCTGCGAC GACTACGAGA CAAACATCTT 
AAAGAAATTC AAGACCTGCA GAGTCGCCAG AAGCATGAAA TTGAATCTTT GTATACTAAA 
CTGGGCAAGG TTCCTCCTGC TGTCATTATT CCCCCAGCGG CCCCCCTTTC AGGAAGAAGA 
AGGCGACCCA CTAAAAGCAA AGGCAGCAAA TCTAGTCGTA TCAGTTCCTT GGGGAGTAAA 
AGCCCCCAGC TTTCAGGTAA TATGTCTGGT CAGAGTGAAG CTTCAGTCTT ACACCCCCAG 
CAGACCCTCC ACCCTCCAGG CAACATCCCA GAAACTGGGC AGAATCAGTT GTTACAGCCC 
CTTAAGCCAT CTCCCTCCAG TGACAACCTG TATTCAGCCT TCACCAGTGA TGGTGCCATT 
TCAGTACCAA GCCTTTCTGC TCCAGGTCAA GGGACCAGCA GCACAAACAC TGTCGGGGGA 
ACAGTGAATA GCCAAGCTGC CCAAGCTCAG CCTCCTGCCA TGACGTCCAG CAGGAAGGGC 
ACGTTCACAG ATGACCTGCA CAAGTTGGTA GACAATTGGG CCAGAGATGC CATGAATCTT 
TCAGGCAGGA GAGGAAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGGAAG 
TTCTCTGCAC CTGGTCAGCT GTGCATCTCC ATGACCTCAA ATCTGGGTGG CTCTGCCCCC 
ATCTCTGCAG CATCAGCTAC CTCTCTAGGT CACTTCACCA AGTCTATGTG CCCCCCACAG 
CAGTATGGTT TTCCACCTAC CCCCTTTGGC ACTCAGTGGA GTGGGACAGG TGGCCCAGCA 
CCACAGCCAC TTGGCCAGTT CCAACCTGTG GGAACCACCT CCTTACAGAA TTTCAACATC 
AGCAATTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011367830.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011366132.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

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CloneID	OPt52300
Clone ID Related Accession (Same CDS sequence)	XM_011367830.1 , XM_011367830.2
Accession Version	XM_011367830.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGCC TCAGTCCATG 
GCGCATCCGT GTGGGGGGAC CCCAACATAC CCAGAATCAC AGATATTTTT CCCAACTATT 
CATGAACGTC CAGTTTCTTT TTCACCACCT CCCACCTGTC CACCGAAGGT GGCCATTTCC 
CAGCGACGTA AGAGCACCTC CTTCCTGGAA GCTCAAACTC ACCACTTCCA ACCCCTGCTG 
AGGACTGTTG GCCAAAATCT TCTTCCACCT GGTGGCAGCC CAACTAACTG GACACCAGAG 
GCCGTAGTTA TGCTGGGTTC TACAACCAGT AGAATATCCG GAGAGCCATG TGATATACAG 
GTCAATCCTA TATTTGAACC AGCTCAAGTT TACAGTGACT ATAGACCTGG ACTAGTACTT 
CCAGAAGAAG CTCACTATTT TATTCCTCAG GAAGCAGTGT ATCTAGCTGG GGTACAGTAC 
CAGGCCCAGG TGGCAGAACA GTATGAGGGT ATTCCATACA ACTCACCAGT ACTGTCAAGT 
CCTATGAAAC AGATACCTGA ACAGAAGCCA GTACAAGGGG GCCCTACTTC AAGTTCTGTC 
TTTGAATTTC CATCTGGACA GGCTTTCTTG GTAGGACACC TTCAGAATTT AAGATTAGAT 
TCTGGATTGA GTCCAGGATC TCCCCTCTCT AGTATTTCTG CACCTATCAG TACAGATGCT 
ACACGTTTGA AATTTCACCC TGTCTTTGTT CCTCATTCTG CACCCGCTGT GTTAACTCAT 
AACAGTGAGA ACAGAAGCAA TTGTGTATTT GAATTTCATG TTCACACTCC AAGCTCCTCT 
TCAGGAGAAG GAGGAGGAGT TTTACCTCAG CGTGTTTACC GAAATCGACA GGTTGCGGTG 
GACTTGAATC AGGAAGAACC ACCTCCTCAA TCAGTTGGAG TACATGGCCG CCTGCAGCCT 
GTGACTGAAG AACAGCATAA TTACCATGTC CCAGAATTGA CTGTTTCTGT GGTAGAGCCT 
ATCGGACAGA ACTGGCCAAT AGGAAGCCCA GAATATTCCA GTGATTCCTC ACAAATTACT 
TCTTCAGACC CCAGTGATTT TCAGTCACCT CCCCCTACAG GGGGAGCACC TGCACCTTTT 
GGCTCTGACG TCTCATTACC CTTTATCCGT CTACCTCAGA CAGTGTTACA AGAATCCCCA 
CTTTTCTTCT GTTTCCCCCA AGGAACCACA TCTCAGCAGA TTTTATCTGC CTCATTTTCT 
TCAGGAGGAT CTGCACTCCA TCCACAGGCA CAGGGGCAGA GCCAGGGTCA ACCATCCTCA 
AGTAGTGTAA CAGGAGTTTC ATCTTCCCAA TCTATACAAC ATCCTCAGCA GCAGCATGGA 
ATACAGCAGA CAGCCCCTCC TCAACAGACA GTACAGTATT CACTTCCACA GACATCAACC 
TCCAGTGAGG CCACTACTGC ACAGCCAGTG AGTCAGCCTC AGGCTCCACA GGTCTTGCCT 
CAAGCATCAG CTGGAAAACA GCTTCCAGTT TCCCAGCCAG TACCAACTGT CCAAGGCGAA 
CCTCAGATCT CAGTTGCGAC ACAACCCTCA GTTGTTCCAG TGCATTCTGG TGCTCATTTC 
CTCCCTATGG GACAGCCACT TCCTACTTCC TTACTCCCTC AGTACCCTGT CTCTCAAATT 
CCTGTATCAA CTCCTCATGT GTCCGCGGCT CAGACAGGTT TCTCAACCCT TCCCATCACA 
ATTGCAGCTG GCCTTAATCA GCCTCTGCTC ACCTTGGCTT CATCTGCTAC AGCAGCCGCA 
ATCCCAGGGG GATCAACTAT GGCTCCTAGT CAGCTACCAG CCCTTCTGCA GCCTGTGACT 
CAGCTGCCAA GTCAGGTTCA CCCACAGCTC CTGCAGCCAG CAGTTCAGTC CATGGGAATA 
CCAGCTAACC TTGGACAAGC TGCTGAGGTT CCACTTCCCT CTGGAGATGT TCTATACCAG 
GGCTTCCCAC CTCGACTGCC ACCACAATAC CCAGGAGATT CAAATATTGC TCCCTCTTCC 
AGCGTGGCTT CTGTTTGCAT TCCTTCTACA GTCTTATCCC CTCCAATGCC GACAGAAGCA 
TTAGCTACAC CAGGTTACTT TCCTACAGTG GTGCAGCCTT ATATGGAATC AAATCTTTTG 
GTTCCTGTGG GCAGTGTAGG AGGACAGGTT CAAGTGTCCC AACCAGCAGT GAGTTTAGCA 
CAAGCCCCCA CTGCATCCTC CCAGCAAGCA GCTCTGGAGA GTACTCAGGG AGTCTCTCAG 
GTTGCTCCTC CAGAGCCAGT TTCGGTAGCA CAGCCACAGC CCACCCAGCC TGCCACTTTG 
GTCTCTTCTA TAGATAGTGC ACATTCAGAT GTTGCTTCAG GTATGAGCGA TGGCAATGAG 
AATGTGCCAT CATCTGGTGG AAGGCATGAA GGGAGAACTG CAAAACGGCA TTATCGAAAA 
TCTGTAAGAA GTCGCTCTCG TCATGAAAAG ACTTCACGCC CCAAATTGAG AATTTTGAAT 
GTTTCAAATA AAGGAGACCG GGTAGTAGAA TGTCAGTTAG AGACTCACAA TCGGAAAATG 
GTTACATTCA AATTTGATTT AGATGGTGAC AATCCCGAGG AGATAGCAAC AATTATGGTG 
AACAATGACT TTATTCTAGC AATAGAGAGA GAGTCGTTTG TGGATCAAGT ACGGGAAATT 
ATTGAAAAAG CTGATGAAAT GCTCAGTGAG GATGTCAGTG TGGAACCCGA GGGTGACCAG 
GGATTGGAGA GTCTACAGGG AAAGGATGAC TATGGCTTTT CAGGTTCTCA GAAATTGGAA 
GGAGAGTTCA AACAACCACT TCCTGCATCT TCCATGCCAC AGCAAATAGG CATTCCTACC 
AGTTCTTTAA CTCAAGTTGT TCATTCTGCT GGAAGACGGT TTATAGTGAG TCCTGTACCA 
GAAAGCCGAT TACGAGAATC AAAAATTTTC ACCAGTGAAA TATCAGATAC GGTTGCCGCC 
TCTACATCTC ATGGCCCTGG GGTGAACTTG TCCCACTCTG CTTCTTCCCT TAGTCTACAG 
CAGGCCTTTT CTGAACTTAG ACATGCCCAA GTTGCAGAGG GACCCAATAC AGCACCTCCC 
AACTTCAGTC ATATAGGACC AACATTTCCA GTAGTACCTC CTTCCATGAG TAGCATTGCT 
GGAATCCCAA CGACAGCAGC AGCCGTACCT TCAATATCAG TCCCTGTAAC TAGCTGCCCT 
CTTAATGACA TTTCCACATC AATAATTCAA TCTGAGATTA CAGTGCCCAC TGAAAAAGGA 
GTTGCTGGAG TTGCCACCTG CACAGGTGTA ATAACTTCAA GTGGTTTCCC AGTACCACCT 
GCGTCTGAAT CACCAATTCT TTCCAGTGTG GTTTCAAGTA TCACAATACC AACAGTTGTC 
TCAGTATCAA CAACATCCCA GTCAGTTCAG GCTCCCACAT CTGGGAGCGT TGTTTCTAGT 
ATAGGCACTT TCCCTTCTAT ACCAGTTTCA GCAACTTCAG CCTCTGCAGT GGGCAGTGCT 
GCTGCCCCAG GGGCTAAGTC TCCACCTTTG TTATCTGAGC AGTCAGCAGG CATTACTACA 
GGGATAGCCA CATTAACATC AGTTTCTACC ACCACTCCAT TCCCAAGCAC AGCTTCACAG 
CTATCTCTTC AGCTTAGCAG CAGCACCTCT GCTCCTACTC TAGCTGAAAC AGTGGTGGTT 
AGTGCACACT CACTAGATAA AACATCTCAT AGCAGTACAA CTGGACTGGC TTTGTCCCAC 
TCTGCATCAT CTTCCACTTC TTCCCCTGGA GCAGGAGTAT CTAGTTCTAT TTCTCAACCT 
GGTGGATTAC ATCCTTTGGT CATCCCATCA GCTGTAGTTT CTACTCCTGT CCTTTCCCAA 
GCATCAGGAC CTATTTCTAC CCCTTTATTA CCCCAAGGAC CTAGCATCCC ACCATTGGTG 
CAACCTGTTG CCAATGTGCC TGCTGTACAG CAGACACTAA TTCATAGTCA GCCTCAACCA 
GCTTTGCTTC TCAACCAGCC CCATACGCAC TGCCCTGAAA TAGATGCTGA TACACAACCC 
AAAGCTCCTG GGATTGATGA CATAAAGACT CTAGAGGAAA AGTTGCGGTC TCTCTTTAGT 
GAACACAGCT CATCTGGCGC TCAACATGCC TCTGTTGCGC TGGAGACATC ACTAGTAGTA 
GAGACCAGTG TCACACCAGG CATCCCAACC ACTGCTGTTG CACCCAGCAA ACTCATGACT 
TCAACTACAA GTACTTGCTT ACCACCAACC AGTTTGCCAT TAGGAACAGC TGGTTTGTCC 
ATTACACCAC TGGTCACAAC TGGGCAAGTT TCTACCCCAG TCAGCACTAC ATCAGGAGTG 
AAACCTGGAA CTGCTCCATC AAAGCTACCT TTAACTAAGG CTCCAGTGCT GCCAGTGGGT 
ACTGAACTTC CAGCTGGTAC TCCATCCAGT GAGCAGCTGC CACCTATTCC AGGACCTTCA 
CTAACTCAGT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTAAGAAG AGCACTTAGT 
CCAGAGGCTA TTGCGGTGAC ATCCGCAGTT GGTCCTGTGT CCGTGGTGGC TCCAGCGGCA 
GTTACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AAGTCACAGA AGGTTCTGTC 
CCAGCAGCTA GTTCAGGAAC TGGTGTTTTT AAGACGGGGC GATTTCAGGT TTCAGTTGCA 
ATAGATGATT CCCAGAAAGA GGGTAAAACT AAGTCAGAAG ATGCAAAGTC TGTTCATTTT 
GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAGAG TACCCTGGTG 
AAGCCAGACT CTAATGGGGT AACCATCCAT GGCATCTCTT CAGATATGCC AGATAGTGCC 
CACAAAACTC CTGCCGCAGA AGCAAAGTCA GAAACTGGGC AACCTACCAA GGTTGGGCGT 
TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTATCAAG AACTGAAGAC 
AGGCTCACTG AGGCAAAGAA AGAGGGACCG ACAGCATCTC CTCCTTTTAT GGATTTGGAA 
CAACCTGTTC TTCCTGCTGC AATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA 
CATCTGAATG GGCCATCTTC TGATTTGGAG GCTGCCTTCC TAAGTAGGGA TGTAGATGAT 
GGTTCAGGTA GTCCACACTC CCCCCACCAA CTGAGCTCAA AGAGCCTACC TATCCAGAAT 
CTAAGTCAAA GCCTTAGTAA CTCACTCAAC TCCTCTTATA TGAGTAGTGA CAATGAGTCA 
GATATTGAAG ATGAAGACTT AAAGTTAGAG CTGCGACGAC TACGAGACAA ACATCTTAAA 
GAAATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG 
GGCAAGGTTC CTCCTGCTGT CATTATTCCC CCAGCGGCCC CCCTTTCAGG AAGAAGAAGG 
CGACCCACTA AAAGCAAAGG CAGCAAATCT AGTCGTATCA GTTCCTTGGG GAGTAAAAGC 
CCCCAGCTTT CAGGTAATAT GTCTGGTCAG AGTGAAGCTT CAGTCTTACA CCCCCAGCAG 
ACCCTCCACC CTCCAGGCAA CATCCCAGAA ACTGGGCAGA ATCAGTTGTT ACAGCCCCTT 
AAGCCATCTC CCTCCAGTGA CAACCTGTAT TCAGCCTTCA CCAGTGATGG TGCCATTTCA 
GTACCAAGCC TTTCTGCTCC AGGTCAAGGG ACCAGCAGCA CAAACACTGT CGGGGGAACA 
GTGAATAGCC AAGCTGCCCA AGCTCAGCCT CCTGCCATGA CGTCCAGCAG GAAGGGCACG 
TTCACAGATG ACCTGCACAA GTTGGTAGAC AATTGGGCCA GAGATGCCAT GAATCTTTCA 
GGCAGGAGAG GAAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC 
TCTGCACCTG GTCAGCTGTG CATCTCCATG ACCTCAAATC TGGGTGGCTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TATGGTTTTC CACCTACCCC CTTTGGCACT CAGTGGAGTG GGACAGGTGG CCCAGCACCA 
CAGCCACTTG GCCAGTTCCA ACCTGTGGGA ACCACCTCCT TACAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011366132.1
CDS	1014..9407
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQNLLPPGGSPTNWTPEAVVMLGSTTSRISGEPCDIQVNPIFEPAQVYSDYRPGLVLPEEAHYFIPQEAVYLAGVQYQAQVAEQYEGIPYNSPVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLSPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNSENRSNCVFEFHVHTPSSSSGEGGGVLPQRVYRNRQVAVDLNQEEPPPQSVGVHGRLQPVTEEQHNYHVPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAPAPFGSDVSLPFIRLPQTVLQESPLFFCFPQGTTSQQILSASFSSGGSALHPQAQGQSQGQPSSSSVTGVSSSQSIQHPQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPVSQIPVSTPHVSAAQTGFSTLPITIAAGLNQPLLTLASSATAAAIPGGSTMAPSQLPALLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIAPSSSVASVCIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLLVPVGSVGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPEAIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011367830.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011367830.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGCC TCAGTCCATG 
GCGCATCCGT GTGGGGGGAC CCCAACATAC CCAGAATCAC AGATATTTTT CCCAACTATT 
CATGAACGTC CAGTTTCTTT TTCACCACCT CCCACCTGTC CACCGAAGGT GGCCATTTCC 
CAGCGACGTA AGAGCACCTC CTTCCTGGAA GCTCAAACTC ACCACTTCCA ACCCCTGCTG 
AGGACTGTTG GCCAAAATCT TCTTCCACCT GGTGGCAGCC CAACTAACTG GACACCAGAG 
GCCGTAGTTA TGCTGGGTTC TACAACCAGT AGAATATCCG GAGAGCCATG TGATATACAG 
GTCAATCCTA TATTTGAACC AGCTCAAGTT TACAGTGACT ATAGACCTGG ACTAGTACTT 
CCAGAAGAAG CTCACTATTT TATTCCTCAG GAAGCAGTGT ATCTAGCTGG GGTACAGTAC 
CAGGCCCAGG TGGCAGAACA GTATGAGGGT ATTCCATACA ACTCACCAGT ACTGTCAAGT 
CCTATGAAAC AGATACCTGA ACAGAAGCCA GTACAAGGGG GCCCTACTTC AAGTTCTGTC 
TTTGAATTTC CATCTGGACA GGCTTTCTTG GTAGGACACC TTCAGAATTT AAGATTAGAT 
TCTGGATTGA GTCCAGGATC TCCCCTCTCT AGTATTTCTG CACCTATCAG TACAGATGCT 
ACACGTTTGA AATTTCACCC TGTCTTTGTT CCTCATTCTG CACCCGCTGT GTTAACTCAT 
AACAGTGAGA ACAGAAGCAA TTGTGTATTT GAATTTCATG TTCACACTCC AAGCTCCTCT 
TCAGGAGAAG GAGGAGGAGT TTTACCTCAG CGTGTTTACC GAAATCGACA GGTTGCGGTG 
GACTTGAATC AGGAAGAACC ACCTCCTCAA TCAGTTGGAG TACATGGCCG CCTGCAGCCT 
GTGACTGAAG AACAGCATAA TTACCATGTC CCAGAATTGA CTGTTTCTGT GGTAGAGCCT 
ATCGGACAGA ACTGGCCAAT AGGAAGCCCA GAATATTCCA GTGATTCCTC ACAAATTACT 
TCTTCAGACC CCAGTGATTT TCAGTCACCT CCCCCTACAG GGGGAGCACC TGCACCTTTT 
GGCTCTGACG TCTCATTACC CTTTATCCGT CTACCTCAGA CAGTGTTACA AGAATCCCCA 
CTTTTCTTCT GTTTCCCCCA AGGAACCACA TCTCAGCAGA TTTTATCTGC CTCATTTTCT 
TCAGGAGGAT CTGCACTCCA TCCACAGGCA CAGGGGCAGA GCCAGGGTCA ACCATCCTCA 
AGTAGTGTAA CAGGAGTTTC ATCTTCCCAA TCTATACAAC ATCCTCAGCA GCAGCATGGA 
ATACAGCAGA CAGCCCCTCC TCAACAGACA GTACAGTATT CACTTCCACA GACATCAACC 
TCCAGTGAGG CCACTACTGC ACAGCCAGTG AGTCAGCCTC AGGCTCCACA GGTCTTGCCT 
CAAGCATCAG CTGGAAAACA GCTTCCAGTT TCCCAGCCAG TACCAACTGT CCAAGGCGAA 
CCTCAGATCT CAGTTGCGAC ACAACCCTCA GTTGTTCCAG TGCATTCTGG TGCTCATTTC 
CTCCCTATGG GACAGCCACT TCCTACTTCC TTACTCCCTC AGTACCCTGT CTCTCAAATT 
CCTGTATCAA CTCCTCATGT GTCCGCGGCT CAGACAGGTT TCTCAACCCT TCCCATCACA 
ATTGCAGCTG GCCTTAATCA GCCTCTGCTC ACCTTGGCTT CATCTGCTAC AGCAGCCGCA 
ATCCCAGGGG GATCAACTAT GGCTCCTAGT CAGCTACCAG CCCTTCTGCA GCCTGTGACT 
CAGCTGCCAA GTCAGGTTCA CCCACAGCTC CTGCAGCCAG CAGTTCAGTC CATGGGAATA 
CCAGCTAACC TTGGACAAGC TGCTGAGGTT CCACTTCCCT CTGGAGATGT TCTATACCAG 
GGCTTCCCAC CTCGACTGCC ACCACAATAC CCAGGAGATT CAAATATTGC TCCCTCTTCC 
AGCGTGGCTT CTGTTTGCAT TCCTTCTACA GTCTTATCCC CTCCAATGCC GACAGAAGCA 
TTAGCTACAC CAGGTTACTT TCCTACAGTG GTGCAGCCTT ATATGGAATC AAATCTTTTG 
GTTCCTGTGG GCAGTGTAGG AGGACAGGTT CAAGTGTCCC AACCAGCAGT GAGTTTAGCA 
CAAGCCCCCA CTGCATCCTC CCAGCAAGCA GCTCTGGAGA GTACTCAGGG AGTCTCTCAG 
GTTGCTCCTC CAGAGCCAGT TTCGGTAGCA CAGCCACAGC CCACCCAGCC TGCCACTTTG 
GTCTCTTCTA TAGATAGTGC ACATTCAGAT GTTGCTTCAG GTATGAGCGA TGGCAATGAG 
AATGTGCCAT CATCTGGTGG AAGGCATGAA GGGAGAACTG CAAAACGGCA TTATCGAAAA 
TCTGTAAGAA GTCGCTCTCG TCATGAAAAG ACTTCACGCC CCAAATTGAG AATTTTGAAT 
GTTTCAAATA AAGGAGACCG GGTAGTAGAA TGTCAGTTAG AGACTCACAA TCGGAAAATG 
GTTACATTCA AATTTGATTT AGATGGTGAC AATCCCGAGG AGATAGCAAC AATTATGGTG 
AACAATGACT TTATTCTAGC AATAGAGAGA GAGTCGTTTG TGGATCAAGT ACGGGAAATT 
ATTGAAAAAG CTGATGAAAT GCTCAGTGAG GATGTCAGTG TGGAACCCGA GGGTGACCAG 
GGATTGGAGA GTCTACAGGG AAAGGATGAC TATGGCTTTT CAGGTTCTCA GAAATTGGAA 
GGAGAGTTCA AACAACCACT TCCTGCATCT TCCATGCCAC AGCAAATAGG CATTCCTACC 
AGTTCTTTAA CTCAAGTTGT TCATTCTGCT GGAAGACGGT TTATAGTGAG TCCTGTACCA 
GAAAGCCGAT TACGAGAATC AAAAATTTTC ACCAGTGAAA TATCAGATAC GGTTGCCGCC 
TCTACATCTC ATGGCCCTGG GGTGAACTTG TCCCACTCTG CTTCTTCCCT TAGTCTACAG 
CAGGCCTTTT CTGAACTTAG ACATGCCCAA GTTGCAGAGG GACCCAATAC AGCACCTCCC 
AACTTCAGTC ATATAGGACC AACATTTCCA GTAGTACCTC CTTCCATGAG TAGCATTGCT 
GGAATCCCAA CGACAGCAGC AGCCGTACCT TCAATATCAG TCCCTGTAAC TAGCTGCCCT 
CTTAATGACA TTTCCACATC AATAATTCAA TCTGAGATTA CAGTGCCCAC TGAAAAAGGA 
GTTGCTGGAG TTGCCACCTG CACAGGTGTA ATAACTTCAA GTGGTTTCCC AGTACCACCT 
GCGTCTGAAT CACCAATTCT TTCCAGTGTG GTTTCAAGTA TCACAATACC AACAGTTGTC 
TCAGTATCAA CAACATCCCA GTCAGTTCAG GCTCCCACAT CTGGGAGCGT TGTTTCTAGT 
ATAGGCACTT TCCCTTCTAT ACCAGTTTCA GCAACTTCAG CCTCTGCAGT GGGCAGTGCT 
GCTGCCCCAG GGGCTAAGTC TCCACCTTTG TTATCTGAGC AGTCAGCAGG CATTACTACA 
GGGATAGCCA CATTAACATC AGTTTCTACC ACCACTCCAT TCCCAAGCAC AGCTTCACAG 
CTATCTCTTC AGCTTAGCAG CAGCACCTCT GCTCCTACTC TAGCTGAAAC AGTGGTGGTT 
AGTGCACACT CACTAGATAA AACATCTCAT AGCAGTACAA CTGGACTGGC TTTGTCCCAC 
TCTGCATCAT CTTCCACTTC TTCCCCTGGA GCAGGAGTAT CTAGTTCTAT TTCTCAACCT 
GGTGGATTAC ATCCTTTGGT CATCCCATCA GCTGTAGTTT CTACTCCTGT CCTTTCCCAA 
GCATCAGGAC CTATTTCTAC CCCTTTATTA CCCCAAGGAC CTAGCATCCC ACCATTGGTG 
CAACCTGTTG CCAATGTGCC TGCTGTACAG CAGACACTAA TTCATAGTCA GCCTCAACCA 
GCTTTGCTTC TCAACCAGCC CCATACGCAC TGCCCTGAAA TAGATGCTGA TACACAACCC 
AAAGCTCCTG GGATTGATGA CATAAAGACT CTAGAGGAAA AGTTGCGGTC TCTCTTTAGT 
GAACACAGCT CATCTGGCGC TCAACATGCC TCTGTTGCGC TGGAGACATC ACTAGTAGTA 
GAGACCAGTG TCACACCAGG CATCCCAACC ACTGCTGTTG CACCCAGCAA ACTCATGACT 
TCAACTACAA GTACTTGCTT ACCACCAACC AGTTTGCCAT TAGGAACAGC TGGTTTGTCC 
ATTACACCAC TGGTCACAAC TGGGCAAGTT TCTACCCCAG TCAGCACTAC ATCAGGAGTG 
AAACCTGGAA CTGCTCCATC AAAGCTACCT TTAACTAAGG CTCCAGTGCT GCCAGTGGGT 
ACTGAACTTC CAGCTGGTAC TCCATCCAGT GAGCAGCTGC CACCTATTCC AGGACCTTCA 
CTAACTCAGT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTAAGAAG AGCACTTAGT 
CCAGAGGCTA TTGCGGTGAC ATCCGCAGTT GGTCCTGTGT CCGTGGTGGC TCCAGCGGCA 
GTTACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AAGTCACAGA AGGTTCTGTC 
CCAGCAGCTA GTTCAGGAAC TGGTGTTTTT AAGACGGGGC GATTTCAGGT TTCAGTTGCA 
ATAGATGATT CCCAGAAAGA GGGTAAAACT AAGTCAGAAG ATGCAAAGTC TGTTCATTTT 
GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAGAG TACCCTGGTG 
AAGCCAGACT CTAATGGGGT AACCATCCAT GGCATCTCTT CAGATATGCC AGATAGTGCC 
CACAAAACTC CTGCCGCAGA AGCAAAGTCA GAAACTGGGC AACCTACCAA GGTTGGGCGT 
TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTATCAAG AACTGAAGAC 
AGGCTCACTG AGGCAAAGAA AGAGGGACCG ACAGCATCTC CTCCTTTTAT GGATTTGGAA 
CAACCTGTTC TTCCTGCTGC AATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA 
CATCTGAATG GGCCATCTTC TGATTTGGAG GCTGCCTTCC TAAGTAGGGA TGTAGATGAT 
GGTTCAGGTA GTCCACACTC CCCCCACCAA CTGAGCTCAA AGAGCCTACC TATCCAGAAT 
CTAAGTCAAA GCCTTAGTAA CTCACTCAAC TCCTCTTATA TGAGTAGTGA CAATGAGTCA 
GATATTGAAG ATGAAGACTT AAAGTTAGAG CTGCGACGAC TACGAGACAA ACATCTTAAA 
GAAATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG 
GGCAAGGTTC CTCCTGCTGT CATTATTCCC CCAGCGGCCC CCCTTTCAGG AAGAAGAAGG 
CGACCCACTA AAAGCAAAGG CAGCAAATCT AGTCGTATCA GTTCCTTGGG GAGTAAAAGC 
CCCCAGCTTT CAGGTAATAT GTCTGGTCAG AGTGAAGCTT CAGTCTTACA CCCCCAGCAG 
ACCCTCCACC CTCCAGGCAA CATCCCAGAA ACTGGGCAGA ATCAGTTGTT ACAGCCCCTT 
AAGCCATCTC CCTCCAGTGA CAACCTGTAT TCAGCCTTCA CCAGTGATGG TGCCATTTCA 
GTACCAAGCC TTTCTGCTCC AGGTCAAGGG ACCAGCAGCA CAAACACTGT CGGGGGAACA 
GTGAATAGCC AAGCTGCCCA AGCTCAGCCT CCTGCCATGA CGTCCAGCAG GAAGGGCACG 
TTCACAGATG ACCTGCACAA GTTGGTAGAC AATTGGGCCA GAGATGCCAT GAATCTTTCA 
GGCAGGAGAG GAAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC 
TCTGCACCTG GTCAGCTGTG CATCTCCATG ACCTCAAATC TGGGTGGCTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TATGGTTTTC CACCTACCCC CTTTGGCACT CAGTGGAGTG GGACAGGTGG CCCAGCACCA 
CAGCCACTTG GCCAGTTCCA ACCTGTGGGA ACCACCTCCT TACAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011367830.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011366132.1 Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

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CloneID	OPt52300
Clone ID Related Accession (Same CDS sequence)	XM_011367830.1 , XM_011367830.2
Accession Version	XM_011367830.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase WNK1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888918.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011367830.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGCC TCAGTCCATG 
GCGCATCCGT GTGGGGGGAC CCCAACATAC CCAGAATCAC AGATATTTTT CCCAACTATT 
CATGAACGTC CAGTTTCTTT TTCACCACCT CCCACCTGTC CACCGAAGGT GGCCATTTCC 
CAGCGACGTA AGAGCACCTC CTTCCTGGAA GCTCAAACTC ACCACTTCCA ACCCCTGCTG 
AGGACTGTTG GCCAAAATCT TCTTCCACCT GGTGGCAGCC CAACTAACTG GACACCAGAG 
GCCGTAGTTA TGCTGGGTTC TACAACCAGT AGAATATCCG GAGAGCCATG TGATATACAG 
GTCAATCCTA TATTTGAACC AGCTCAAGTT TACAGTGACT ATAGACCTGG ACTAGTACTT 
CCAGAAGAAG CTCACTATTT TATTCCTCAG GAAGCAGTGT ATCTAGCTGG GGTACAGTAC 
CAGGCCCAGG TGGCAGAACA GTATGAGGGT ATTCCATACA ACTCACCAGT ACTGTCAAGT 
CCTATGAAAC AGATACCTGA ACAGAAGCCA GTACAAGGGG GCCCTACTTC AAGTTCTGTC 
TTTGAATTTC CATCTGGACA GGCTTTCTTG GTAGGACACC TTCAGAATTT AAGATTAGAT 
TCTGGATTGA GTCCAGGATC TCCCCTCTCT AGTATTTCTG CACCTATCAG TACAGATGCT 
ACACGTTTGA AATTTCACCC TGTCTTTGTT CCTCATTCTG CACCCGCTGT GTTAACTCAT 
AACAGTGAGA ACAGAAGCAA TTGTGTATTT GAATTTCATG TTCACACTCC AAGCTCCTCT 
TCAGGAGAAG GAGGAGGAGT TTTACCTCAG CGTGTTTACC GAAATCGACA GGTTGCGGTG 
GACTTGAATC AGGAAGAACC ACCTCCTCAA TCAGTTGGAG TACATGGCCG CCTGCAGCCT 
GTGACTGAAG AACAGCATAA TTACCATGTC CCAGAATTGA CTGTTTCTGT GGTAGAGCCT 
ATCGGACAGA ACTGGCCAAT AGGAAGCCCA GAATATTCCA GTGATTCCTC ACAAATTACT 
TCTTCAGACC CCAGTGATTT TCAGTCACCT CCCCCTACAG GGGGAGCACC TGCACCTTTT 
GGCTCTGACG TCTCATTACC CTTTATCCGT CTACCTCAGA CAGTGTTACA AGAATCCCCA 
CTTTTCTTCT GTTTCCCCCA AGGAACCACA TCTCAGCAGA TTTTATCTGC CTCATTTTCT 
TCAGGAGGAT CTGCACTCCA TCCACAGGCA CAGGGGCAGA GCCAGGGTCA ACCATCCTCA 
AGTAGTGTAA CAGGAGTTTC ATCTTCCCAA TCTATACAAC ATCCTCAGCA GCAGCATGGA 
ATACAGCAGA CAGCCCCTCC TCAACAGACA GTACAGTATT CACTTCCACA GACATCAACC 
TCCAGTGAGG CCACTACTGC ACAGCCAGTG AGTCAGCCTC AGGCTCCACA GGTCTTGCCT 
CAAGCATCAG CTGGAAAACA GCTTCCAGTT TCCCAGCCAG TACCAACTGT CCAAGGCGAA 
CCTCAGATCT CAGTTGCGAC ACAACCCTCA GTTGTTCCAG TGCATTCTGG TGCTCATTTC 
CTCCCTATGG GACAGCCACT TCCTACTTCC TTACTCCCTC AGTACCCTGT CTCTCAAATT 
CCTGTATCAA CTCCTCATGT GTCCGCGGCT CAGACAGGTT TCTCAACCCT TCCCATCACA 
ATTGCAGCTG GCCTTAATCA GCCTCTGCTC ACCTTGGCTT CATCTGCTAC AGCAGCCGCA 
ATCCCAGGGG GATCAACTAT GGCTCCTAGT CAGCTACCAG CCCTTCTGCA GCCTGTGACT 
CAGCTGCCAA GTCAGGTTCA CCCACAGCTC CTGCAGCCAG CAGTTCAGTC CATGGGAATA 
CCAGCTAACC TTGGACAAGC TGCTGAGGTT CCACTTCCCT CTGGAGATGT TCTATACCAG 
GGCTTCCCAC CTCGACTGCC ACCACAATAC CCAGGAGATT CAAATATTGC TCCCTCTTCC 
AGCGTGGCTT CTGTTTGCAT TCCTTCTACA GTCTTATCCC CTCCAATGCC GACAGAAGCA 
TTAGCTACAC CAGGTTACTT TCCTACAGTG GTGCAGCCTT ATATGGAATC AAATCTTTTG 
GTTCCTGTGG GCAGTGTAGG AGGACAGGTT CAAGTGTCCC AACCAGCAGT GAGTTTAGCA 
CAAGCCCCCA CTGCATCCTC CCAGCAAGCA GCTCTGGAGA GTACTCAGGG AGTCTCTCAG 
GTTGCTCCTC CAGAGCCAGT TTCGGTAGCA CAGCCACAGC CCACCCAGCC TGCCACTTTG 
GTCTCTTCTA TAGATAGTGC ACATTCAGAT GTTGCTTCAG GTATGAGCGA TGGCAATGAG 
AATGTGCCAT CATCTGGTGG AAGGCATGAA GGGAGAACTG CAAAACGGCA TTATCGAAAA 
TCTGTAAGAA GTCGCTCTCG TCATGAAAAG ACTTCACGCC CCAAATTGAG AATTTTGAAT 
GTTTCAAATA AAGGAGACCG GGTAGTAGAA TGTCAGTTAG AGACTCACAA TCGGAAAATG 
GTTACATTCA AATTTGATTT AGATGGTGAC AATCCCGAGG AGATAGCAAC AATTATGGTG 
AACAATGACT TTATTCTAGC AATAGAGAGA GAGTCGTTTG TGGATCAAGT ACGGGAAATT 
ATTGAAAAAG CTGATGAAAT GCTCAGTGAG GATGTCAGTG TGGAACCCGA GGGTGACCAG 
GGATTGGAGA GTCTACAGGG AAAGGATGAC TATGGCTTTT CAGGTTCTCA GAAATTGGAA 
GGAGAGTTCA AACAACCACT TCCTGCATCT TCCATGCCAC AGCAAATAGG CATTCCTACC 
AGTTCTTTAA CTCAAGTTGT TCATTCTGCT GGAAGACGGT TTATAGTGAG TCCTGTACCA 
GAAAGCCGAT TACGAGAATC AAAAATTTTC ACCAGTGAAA TATCAGATAC GGTTGCCGCC 
TCTACATCTC ATGGCCCTGG GGTGAACTTG TCCCACTCTG CTTCTTCCCT TAGTCTACAG 
CAGGCCTTTT CTGAACTTAG ACATGCCCAA GTTGCAGAGG GACCCAATAC AGCACCTCCC 
AACTTCAGTC ATATAGGACC AACATTTCCA GTAGTACCTC CTTCCATGAG TAGCATTGCT 
GGAATCCCAA CGACAGCAGC AGCCGTACCT TCAATATCAG TCCCTGTAAC TAGCTGCCCT 
CTTAATGACA TTTCCACATC AATAATTCAA TCTGAGATTA CAGTGCCCAC TGAAAAAGGA 
GTTGCTGGAG TTGCCACCTG CACAGGTGTA ATAACTTCAA GTGGTTTCCC AGTACCACCT 
GCGTCTGAAT CACCAATTCT TTCCAGTGTG GTTTCAAGTA TCACAATACC AACAGTTGTC 
TCAGTATCAA CAACATCCCA GTCAGTTCAG GCTCCCACAT CTGGGAGCGT TGTTTCTAGT 
ATAGGCACTT TCCCTTCTAT ACCAGTTTCA GCAACTTCAG CCTCTGCAGT GGGCAGTGCT 
GCTGCCCCAG GGGCTAAGTC TCCACCTTTG TTATCTGAGC AGTCAGCAGG CATTACTACA 
GGGATAGCCA CATTAACATC AGTTTCTACC ACCACTCCAT TCCCAAGCAC AGCTTCACAG 
CTATCTCTTC AGCTTAGCAG CAGCACCTCT GCTCCTACTC TAGCTGAAAC AGTGGTGGTT 
AGTGCACACT CACTAGATAA AACATCTCAT AGCAGTACAA CTGGACTGGC TTTGTCCCAC 
TCTGCATCAT CTTCCACTTC TTCCCCTGGA GCAGGAGTAT CTAGTTCTAT TTCTCAACCT 
GGTGGATTAC ATCCTTTGGT CATCCCATCA GCTGTAGTTT CTACTCCTGT CCTTTCCCAA 
GCATCAGGAC CTATTTCTAC CCCTTTATTA CCCCAAGGAC CTAGCATCCC ACCATTGGTG 
CAACCTGTTG CCAATGTGCC TGCTGTACAG CAGACACTAA TTCATAGTCA GCCTCAACCA 
GCTTTGCTTC TCAACCAGCC CCATACGCAC TGCCCTGAAA TAGATGCTGA TACACAACCC 
AAAGCTCCTG GGATTGATGA CATAAAGACT CTAGAGGAAA AGTTGCGGTC TCTCTTTAGT 
GAACACAGCT CATCTGGCGC TCAACATGCC TCTGTTGCGC TGGAGACATC ACTAGTAGTA 
GAGACCAGTG TCACACCAGG CATCCCAACC ACTGCTGTTG CACCCAGCAA ACTCATGACT 
TCAACTACAA GTACTTGCTT ACCACCAACC AGTTTGCCAT TAGGAACAGC TGGTTTGTCC 
ATTACACCAC TGGTCACAAC TGGGCAAGTT TCTACCCCAG TCAGCACTAC ATCAGGAGTG 
AAACCTGGAA CTGCTCCATC AAAGCTACCT TTAACTAAGG CTCCAGTGCT GCCAGTGGGT 
ACTGAACTTC CAGCTGGTAC TCCATCCAGT GAGCAGCTGC CACCTATTCC AGGACCTTCA 
CTAACTCAGT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTAAGAAG AGCACTTAGT 
CCAGAGGCTA TTGCGGTGAC ATCCGCAGTT GGTCCTGTGT CCGTGGTGGC TCCAGCGGCA 
GTTACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AAGTCACAGA AGGTTCTGTC 
CCAGCAGCTA GTTCAGGAAC TGGTGTTTTT AAGACGGGGC GATTTCAGGT TTCAGTTGCA 
ATAGATGATT CCCAGAAAGA GGGTAAAACT AAGTCAGAAG ATGCAAAGTC TGTTCATTTT 
GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAGAG TACCCTGGTG 
AAGCCAGACT CTAATGGGGT AACCATCCAT GGCATCTCTT CAGATATGCC AGATAGTGCC 
CACAAAACTC CTGCCGCAGA AGCAAAGTCA GAAACTGGGC AACCTACCAA GGTTGGGCGT 
TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTATCAAG AACTGAAGAC 
AGGCTCACTG AGGCAAAGAA AGAGGGACCG ACAGCATCTC CTCCTTTTAT GGATTTGGAA 
CAACCTGTTC TTCCTGCTGC AATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA 
CATCTGAATG GGCCATCTTC TGATTTGGAG GCTGCCTTCC TAAGTAGGGA TGTAGATGAT 
GGTTCAGGTA GTCCACACTC CCCCCACCAA CTGAGCTCAA AGAGCCTACC TATCCAGAAT 
CTAAGTCAAA GCCTTAGTAA CTCACTCAAC TCCTCTTATA TGAGTAGTGA CAATGAGTCA 
GATATTGAAG ATGAAGACTT AAAGTTAGAG CTGCGACGAC TACGAGACAA ACATCTTAAA 
GAAATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG 
GGCAAGGTTC CTCCTGCTGT CATTATTCCC CCAGCGGCCC CCCTTTCAGG AAGAAGAAGG 
CGACCCACTA AAAGCAAAGG CAGCAAATCT AGTCGTATCA GTTCCTTGGG GAGTAAAAGC 
CCCCAGCTTT CAGGTAATAT GTCTGGTCAG AGTGAAGCTT CAGTCTTACA CCCCCAGCAG 
ACCCTCCACC CTCCAGGCAA CATCCCAGAA ACTGGGCAGA ATCAGTTGTT ACAGCCCCTT 
AAGCCATCTC CCTCCAGTGA CAACCTGTAT TCAGCCTTCA CCAGTGATGG TGCCATTTCA 
GTACCAAGCC TTTCTGCTCC AGGTCAAGGG ACCAGCAGCA CAAACACTGT CGGGGGAACA 
GTGAATAGCC AAGCTGCCCA AGCTCAGCCT CCTGCCATGA CGTCCAGCAG GAAGGGCACG 
TTCACAGATG ACCTGCACAA GTTGGTAGAC AATTGGGCCA GAGATGCCAT GAATCTTTCA 
GGCAGGAGAG GAAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC 
TCTGCACCTG GTCAGCTGTG CATCTCCATG ACCTCAAATC TGGGTGGCTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TATGGTTTTC CACCTACCCC CTTTGGCACT CAGTGGAGTG GGACAGGTGG CCCAGCACCA 
CAGCCACTTG GCCAGTTCCA ACCTGTGGGA ACCACCTCCT TACAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011366132.1
CDS	682..9075
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAASQVAQQSPAAATAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLDRDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQVEQQSSASQAGIKQLPSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASIVQAQSQPHAVYPPSSMPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQNLLPPGGSPTNWTPEAVVMLGSTTSRISGEPCDIQVNPIFEPAQVYSDYRPGLVLPEEAHYFIPQEAVYLAGVQYQAQVAEQYEGIPYNSPVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLSPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNSENRSNCVFEFHVHTPSSSSGEGGGVLPQRVYRNRQVAVDLNQEEPPPQSVGVHGRLQPVTEEQHNYHVPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAPAPFGSDVSLPFIRLPQTVLQESPLFFCFPQGTTSQQILSASFSSGGSALHPQAQGQSQGQPSSSSVTGVSSSQSIQHPQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPVSQIPVSTPHVSAAQTGFSTLPITIAAGLNQPLLTLASSATAAAIPGGSTMAPSQLPALLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIAPSSSVASVCIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLLVPVGSVGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPATLVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEISDTVAASTSHGPGVNLSHSASSLSLQQAFSELRHAQVAEGPNTAPPNFSHIGPTFPVVPPSMSSIAGIPTTAAAVPSISVPVTSCPLNDISTSIIQSEITVPTEKGVAGVATCTGVITSSGFPVPPASESPILSSVVSSITIPTVVSVSTTSQSVQAPTSGSVVSSIGTFPSIPVSATSASAVGSAAAPGAKSPPLLSEQSAGITTGIATLTSVSTTTPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSHSASSSTSSPGAGVSSSISQPGGLHPLVIPSAVVSTPVLSQASGPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLLNQPHTHCPEIDADTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSITPLVTTGQVSTPVSTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPEAIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTEGSVPAASSGTGVFKTGRFQVSVAIDDSQKEGKTKSEDAKSVHFESSTSESSVLSSSSPESTLVKPDSNGVTIHGISSDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRLTEAKKEGPTASPPFMDLEQPVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTTSLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011367830.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011367830.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTTGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAACGCCACT GCATTGGAAC TGCCGGGTCT GCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTTCC CAGGTAGCCC AGCAGTCTCC CGCCGCTGCT 
ACTGCTGTCT CTGGGGAACC GGCTGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGTAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAGG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATTGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TGCAGGACCG AAAGTTATCC 
AAGTCCGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ACCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGGGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCAGAG TTCATGGCCC CTGAAATGTA TGAGGAGAAA 
TACGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGTA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGA 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GACAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACCATGGC GAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGGGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT TTCAAACAGC AAGTGGAACA ACAATCCAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCCCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CTGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACACTCC CAGGACATAC AGCTTCTATT 
GTCCAAGCAC AATCTCAGCC CCATGCTGTA TATCCACCCT CAAGTATGCC TCAGTCCATG 
GCGCATCCGT GTGGGGGGAC CCCAACATAC CCAGAATCAC AGATATTTTT CCCAACTATT 
CATGAACGTC CAGTTTCTTT TTCACCACCT CCCACCTGTC CACCGAAGGT GGCCATTTCC 
CAGCGACGTA AGAGCACCTC CTTCCTGGAA GCTCAAACTC ACCACTTCCA ACCCCTGCTG 
AGGACTGTTG GCCAAAATCT TCTTCCACCT GGTGGCAGCC CAACTAACTG GACACCAGAG 
GCCGTAGTTA TGCTGGGTTC TACAACCAGT AGAATATCCG GAGAGCCATG TGATATACAG 
GTCAATCCTA TATTTGAACC AGCTCAAGTT TACAGTGACT ATAGACCTGG ACTAGTACTT 
CCAGAAGAAG CTCACTATTT TATTCCTCAG GAAGCAGTGT ATCTAGCTGG GGTACAGTAC 
CAGGCCCAGG TGGCAGAACA GTATGAGGGT ATTCCATACA ACTCACCAGT ACTGTCAAGT 
CCTATGAAAC AGATACCTGA ACAGAAGCCA GTACAAGGGG GCCCTACTTC AAGTTCTGTC 
TTTGAATTTC CATCTGGACA GGCTTTCTTG GTAGGACACC TTCAGAATTT AAGATTAGAT 
TCTGGATTGA GTCCAGGATC TCCCCTCTCT AGTATTTCTG CACCTATCAG TACAGATGCT 
ACACGTTTGA AATTTCACCC TGTCTTTGTT CCTCATTCTG CACCCGCTGT GTTAACTCAT 
AACAGTGAGA ACAGAAGCAA TTGTGTATTT GAATTTCATG TTCACACTCC AAGCTCCTCT 
TCAGGAGAAG GAGGAGGAGT TTTACCTCAG CGTGTTTACC GAAATCGACA GGTTGCGGTG 
GACTTGAATC AGGAAGAACC ACCTCCTCAA TCAGTTGGAG TACATGGCCG CCTGCAGCCT 
GTGACTGAAG AACAGCATAA TTACCATGTC CCAGAATTGA CTGTTTCTGT GGTAGAGCCT 
ATCGGACAGA ACTGGCCAAT AGGAAGCCCA GAATATTCCA GTGATTCCTC ACAAATTACT 
TCTTCAGACC CCAGTGATTT TCAGTCACCT CCCCCTACAG GGGGAGCACC TGCACCTTTT 
GGCTCTGACG TCTCATTACC CTTTATCCGT CTACCTCAGA CAGTGTTACA AGAATCCCCA 
CTTTTCTTCT GTTTCCCCCA AGGAACCACA TCTCAGCAGA TTTTATCTGC CTCATTTTCT 
TCAGGAGGAT CTGCACTCCA TCCACAGGCA CAGGGGCAGA GCCAGGGTCA ACCATCCTCA 
AGTAGTGTAA CAGGAGTTTC ATCTTCCCAA TCTATACAAC ATCCTCAGCA GCAGCATGGA 
ATACAGCAGA CAGCCCCTCC TCAACAGACA GTACAGTATT CACTTCCACA GACATCAACC 
TCCAGTGAGG CCACTACTGC ACAGCCAGTG AGTCAGCCTC AGGCTCCACA GGTCTTGCCT 
CAAGCATCAG CTGGAAAACA GCTTCCAGTT TCCCAGCCAG TACCAACTGT CCAAGGCGAA 
CCTCAGATCT CAGTTGCGAC ACAACCCTCA GTTGTTCCAG TGCATTCTGG TGCTCATTTC 
CTCCCTATGG GACAGCCACT TCCTACTTCC TTACTCCCTC AGTACCCTGT CTCTCAAATT 
CCTGTATCAA CTCCTCATGT GTCCGCGGCT CAGACAGGTT TCTCAACCCT TCCCATCACA 
ATTGCAGCTG GCCTTAATCA GCCTCTGCTC ACCTTGGCTT CATCTGCTAC AGCAGCCGCA 
ATCCCAGGGG GATCAACTAT GGCTCCTAGT CAGCTACCAG CCCTTCTGCA GCCTGTGACT 
CAGCTGCCAA GTCAGGTTCA CCCACAGCTC CTGCAGCCAG CAGTTCAGTC CATGGGAATA 
CCAGCTAACC TTGGACAAGC TGCTGAGGTT CCACTTCCCT CTGGAGATGT TCTATACCAG 
GGCTTCCCAC CTCGACTGCC ACCACAATAC CCAGGAGATT CAAATATTGC TCCCTCTTCC 
AGCGTGGCTT CTGTTTGCAT TCCTTCTACA GTCTTATCCC CTCCAATGCC GACAGAAGCA 
TTAGCTACAC CAGGTTACTT TCCTACAGTG GTGCAGCCTT ATATGGAATC AAATCTTTTG 
GTTCCTGTGG GCAGTGTAGG AGGACAGGTT CAAGTGTCCC AACCAGCAGT GAGTTTAGCA 
CAAGCCCCCA CTGCATCCTC CCAGCAAGCA GCTCTGGAGA GTACTCAGGG AGTCTCTCAG 
GTTGCTCCTC CAGAGCCAGT TTCGGTAGCA CAGCCACAGC CCACCCAGCC TGCCACTTTG 
GTCTCTTCTA TAGATAGTGC ACATTCAGAT GTTGCTTCAG GTATGAGCGA TGGCAATGAG 
AATGTGCCAT CATCTGGTGG AAGGCATGAA GGGAGAACTG CAAAACGGCA TTATCGAAAA 
TCTGTAAGAA GTCGCTCTCG TCATGAAAAG ACTTCACGCC CCAAATTGAG AATTTTGAAT 
GTTTCAAATA AAGGAGACCG GGTAGTAGAA TGTCAGTTAG AGACTCACAA TCGGAAAATG 
GTTACATTCA AATTTGATTT AGATGGTGAC AATCCCGAGG AGATAGCAAC AATTATGGTG 
AACAATGACT TTATTCTAGC AATAGAGAGA GAGTCGTTTG TGGATCAAGT ACGGGAAATT 
ATTGAAAAAG CTGATGAAAT GCTCAGTGAG GATGTCAGTG TGGAACCCGA GGGTGACCAG 
GGATTGGAGA GTCTACAGGG AAAGGATGAC TATGGCTTTT CAGGTTCTCA GAAATTGGAA 
GGAGAGTTCA AACAACCACT TCCTGCATCT TCCATGCCAC AGCAAATAGG CATTCCTACC 
AGTTCTTTAA CTCAAGTTGT TCATTCTGCT GGAAGACGGT TTATAGTGAG TCCTGTACCA 
GAAAGCCGAT TACGAGAATC AAAAATTTTC ACCAGTGAAA TATCAGATAC GGTTGCCGCC 
TCTACATCTC ATGGCCCTGG GGTGAACTTG TCCCACTCTG CTTCTTCCCT TAGTCTACAG 
CAGGCCTTTT CTGAACTTAG ACATGCCCAA GTTGCAGAGG GACCCAATAC AGCACCTCCC 
AACTTCAGTC ATATAGGACC AACATTTCCA GTAGTACCTC CTTCCATGAG TAGCATTGCT 
GGAATCCCAA CGACAGCAGC AGCCGTACCT TCAATATCAG TCCCTGTAAC TAGCTGCCCT 
CTTAATGACA TTTCCACATC AATAATTCAA TCTGAGATTA CAGTGCCCAC TGAAAAAGGA 
GTTGCTGGAG TTGCCACCTG CACAGGTGTA ATAACTTCAA GTGGTTTCCC AGTACCACCT 
GCGTCTGAAT CACCAATTCT TTCCAGTGTG GTTTCAAGTA TCACAATACC AACAGTTGTC 
TCAGTATCAA CAACATCCCA GTCAGTTCAG GCTCCCACAT CTGGGAGCGT TGTTTCTAGT 
ATAGGCACTT TCCCTTCTAT ACCAGTTTCA GCAACTTCAG CCTCTGCAGT GGGCAGTGCT 
GCTGCCCCAG GGGCTAAGTC TCCACCTTTG TTATCTGAGC AGTCAGCAGG CATTACTACA 
GGGATAGCCA CATTAACATC AGTTTCTACC ACCACTCCAT TCCCAAGCAC AGCTTCACAG 
CTATCTCTTC AGCTTAGCAG CAGCACCTCT GCTCCTACTC TAGCTGAAAC AGTGGTGGTT 
AGTGCACACT CACTAGATAA AACATCTCAT AGCAGTACAA CTGGACTGGC TTTGTCCCAC 
TCTGCATCAT CTTCCACTTC TTCCCCTGGA GCAGGAGTAT CTAGTTCTAT TTCTCAACCT 
GGTGGATTAC ATCCTTTGGT CATCCCATCA GCTGTAGTTT CTACTCCTGT CCTTTCCCAA 
GCATCAGGAC CTATTTCTAC CCCTTTATTA CCCCAAGGAC CTAGCATCCC ACCATTGGTG 
CAACCTGTTG CCAATGTGCC TGCTGTACAG CAGACACTAA TTCATAGTCA GCCTCAACCA 
GCTTTGCTTC TCAACCAGCC CCATACGCAC TGCCCTGAAA TAGATGCTGA TACACAACCC 
AAAGCTCCTG GGATTGATGA CATAAAGACT CTAGAGGAAA AGTTGCGGTC TCTCTTTAGT 
GAACACAGCT CATCTGGCGC TCAACATGCC TCTGTTGCGC TGGAGACATC ACTAGTAGTA 
GAGACCAGTG TCACACCAGG CATCCCAACC ACTGCTGTTG CACCCAGCAA ACTCATGACT 
TCAACTACAA GTACTTGCTT ACCACCAACC AGTTTGCCAT TAGGAACAGC TGGTTTGTCC 
ATTACACCAC TGGTCACAAC TGGGCAAGTT TCTACCCCAG TCAGCACTAC ATCAGGAGTG 
AAACCTGGAA CTGCTCCATC AAAGCTACCT TTAACTAAGG CTCCAGTGCT GCCAGTGGGT 
ACTGAACTTC CAGCTGGTAC TCCATCCAGT GAGCAGCTGC CACCTATTCC AGGACCTTCA 
CTAACTCAGT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTAAGAAG AGCACTTAGT 
CCAGAGGCTA TTGCGGTGAC ATCCGCAGTT GGTCCTGTGT CCGTGGTGGC TCCAGCGGCA 
GTTACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AAGTCACAGA AGGTTCTGTC 
CCAGCAGCTA GTTCAGGAAC TGGTGTTTTT AAGACGGGGC GATTTCAGGT TTCAGTTGCA 
ATAGATGATT CCCAGAAAGA GGGTAAAACT AAGTCAGAAG ATGCAAAGTC TGTTCATTTT 
GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAGAG TACCCTGGTG 
AAGCCAGACT CTAATGGGGT AACCATCCAT GGCATCTCTT CAGATATGCC AGATAGTGCC 
CACAAAACTC CTGCCGCAGA AGCAAAGTCA GAAACTGGGC AACCTACCAA GGTTGGGCGT 
TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTATCAAG AACTGAAGAC 
AGGCTCACTG AGGCAAAGAA AGAGGGACCG ACAGCATCTC CTCCTTTTAT GGATTTGGAA 
CAACCTGTTC TTCCTGCTGC AATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA 
CATCTGAATG GGCCATCTTC TGATTTGGAG GCTGCCTTCC TAAGTAGGGA TGTAGATGAT 
GGTTCAGGTA GTCCACACTC CCCCCACCAA CTGAGCTCAA AGAGCCTACC TATCCAGAAT 
CTAAGTCAAA GCCTTAGTAA CTCACTCAAC TCCTCTTATA TGAGTAGTGA CAATGAGTCA 
GATATTGAAG ATGAAGACTT AAAGTTAGAG CTGCGACGAC TACGAGACAA ACATCTTAAA 
GAAATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG 
GGCAAGGTTC CTCCTGCTGT CATTATTCCC CCAGCGGCCC CCCTTTCAGG AAGAAGAAGG 
CGACCCACTA AAAGCAAAGG CAGCAAATCT AGTCGTATCA GTTCCTTGGG GAGTAAAAGC 
CCCCAGCTTT CAGGTAATAT GTCTGGTCAG AGTGAAGCTT CAGTCTTACA CCCCCAGCAG 
ACCCTCCACC CTCCAGGCAA CATCCCAGAA ACTGGGCAGA ATCAGTTGTT ACAGCCCCTT 
AAGCCATCTC CCTCCAGTGA CAACCTGTAT TCAGCCTTCA CCAGTGATGG TGCCATTTCA 
GTACCAAGCC TTTCTGCTCC AGGTCAAGGG ACCAGCAGCA CAAACACTGT CGGGGGAACA 
GTGAATAGCC AAGCTGCCCA AGCTCAGCCT CCTGCCATGA CGTCCAGCAG GAAGGGCACG 
TTCACAGATG ACCTGCACAA GTTGGTAGAC AATTGGGCCA GAGATGCCAT GAATCTTTCA 
GGCAGGAGAG GAAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC 
TCTGCACCTG GTCAGCTGTG CATCTCCATG ACCTCAAATC TGGGTGGCTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TATGGTTTTC CACCTACCCC CTTTGGCACT CAGTGGAGTG GGACAGGTGG CCCAGCACCA 
CAGCCACTTG GCCAGTTCCA ACCTGTGGGA ACCACCTCCT TACAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	WNK1
Entrez Gene ID	105297259
Full Name	WNK lysine deficient protein kinase 1
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

WNK1 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene

Clones

gRNAs

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