RAD50 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAD50
Entrez Gene ID	105293548
Full Name	RAD50 double strand break repair protein
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011362283.1	XP_011360585.1	DNA repair protein RAD50
XM_023531903.1	XP_023387671.1	DNA repair protein RAD50 isoform X2
XM_011362283.2	XP_011360585.1	DNA repair protein RAD50 isoform X1

Pathways from BioSystems
Gene Ontology

The following RAD50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAD50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt48335	XM_011362283.2 Latest version!	Pteropus vampyrus RAD50 double strand break repair protein (RAD50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt70258	XM_023531903.1 Latest version!	Pteropus vampyrus RAD50 double strand break repair protein (RAD50), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OPt48335	XM_011362283.1	Pteropus vampyrus RAD50 homolog (S. cerevisiae) (RAD50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAD50 ( XM_011362283.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011360585.1 Pteropus vampyrus RAD50 homolog (S. cerevisiae) (RAD50), mRNA.

Price & Availability↑

CloneID	OPt48335
Clone ID Related Accession (Same CDS sequence)	XM_011362283.1 , XM_011362283.2
Accession Version	XM_011362283.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	DNA repair protein RAD50
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888855.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGA TCGAAAAGAT GAGCATTCTG GGCGTGCGGA GTTTTGGAAT AGAGGACAAA 
GATAAGCAAA TTATCACTTT CTTCAGTCCC CTCACGATTT TGGTTGGACC CAATGGGGCG 
GGGAAGACGA CCATCATTGA ATGTCTAAAA TATATTTGCA CTGGAGATTT CCCTCCTGGA 
ACCAAAGGGA ATACATTTGT TCATGATCCC AAGATTGCTC AAGAAACAGA TGTTAGAGCC 
CAGATTCGTC TACAGTTTCG TGATGTCAAT GGAGAACTTG TAGCTGTGCA GAGATCTATG 
GTGTGTACTC AGAAAAGCAA AAAGACAGAG TTTAAAACCC TGGAAGGAGT CATTACTAGA 
ACAAAGCATG GTGAAAAGGT CAGTCTCAGC TCTAAGTGTG CAGAAATTGA CCGAGAGATG 
ATAAGTTCTC TTGGGGTTTC CAAGTCTGTG CTAAATAACG TCATTTTCTG TCATCAAGAA 
GATTCTAACT GGCCTTTAAG TGAAGGAAAG GCTTTGAAGC AAAAATTTGA TGAGATTTTT 
TCAGCAACAA GGTACATTAA AGCTTTGGAA ACAATCCGGC AGGTACGTCA GACACAAGGT 
CAGAAAGTAA AAGAATGTCA AACAGAGCTA AAATATCTGA AGCAAAATAA AGAAAAAGCT 
TGTGAGATTC GCAATCAGAT TGCTAGTAAA GAAGACCAGT TAACATCTTC AAAGGAAATT 
GTCAAAGCCT ATGAGAATGA ACTTGATCCA TTGAAGAATC GTCTAAAAGA AATTGAACAA 
AATCTCGCTA AAATAATGAA ACTTGACAAT GAAATTAAAG CCTTGGAAAG CCGAAAGAAG 
CAAATGGAGA AAGATAATAG TGAACTAGAA CAGAAAATGG AAAAGGTTTT TCAAGGGACT 
GATGAGCAAC TAAATGATTT ATATCACAAT CACCAGAGAA CAGTAAGAGA GAAAGAAAGG 
AAATTGGTAG ACTGTCAGCG TGAACTGGAA AAATTAAATA AAGAATCTAG GCTTCTCAAT 
CAAGAAAAGT CAGAACTACT TGTTGAACAG GGTCGTCTAC AGCTACAGGC AGATCGCCAT 
CAAGAAAATA TCCGAGCTAG AGATTCATTA ATTCAATCTT TGGCAACACA GCTAGAATTG 
GATGGCTTTG AGCGTGGACC ATTCAGTGAA AGACAGATTA AAAATTTTCA CAAACTTGTG 
AGAGAGAGAC AAGAAAAAGA AGCAGAAACT GCCAACCAAC TGATGAATGA CTTTGCAGAA 
AAAGAGACTC TGAAACAAAA ACAGATAGAT GAAATAAGGG ATAAGAAAAT TGGACTGGGA 
AGAATAATTG AACTAAAATC AGAAATCCTA AGTAAGAAGC AGAACGAACT GAAAAATGTG 
AAGTACGAAT TACAGCAATT GGAAGGATCT TCAGACAGGA TTCTTGAACT GGACCAGGAG 
CTCACAAAAG CTGAACGTGA GCTAAGCAAG GCTGAGAAAA ATAGCAATAT AGAAACTCTA 
AAAACAGAAG TAATAAGCCT TCAAAATGAG AAAGCAGACC TAGACAAGAC CCTGCGTAAA 
TTGGACCAGG AGATGGAGCA GTTAAACCAT CATACAACAA CGCGCACCCA GATGGAGATG 
TTGACCAAAG ACAAAGCTGA CAAAGATGAA CAAATCAGAA AAATAAAATC TAGGCACAGT 
GATGAATTAA ATTCATTGTT AGGATATTTT CCCAACAAAA AACAGCTTGA AGACTGGCTT 
CATAGTAAAT CAAAAGAAAT TAATCAGACC AGAGACAGAC TTGCCAAATT GAACAAGGAA 
CTAGCTTCAG CTGAGCAAAA TAAAAATCAT ATGAATAATG AGCTAAAAAG GAAGGAAGAG 
CAGTTGTCCA GTTATGAAGA CAAGCTTTTT GATGTTTGTG GTAGCCAGGA TTTTGAAAGT 
GATTTAGACA GACTTAAAGA AGAAATTGAA AAATCCTCAA AACAACGAGC CATGCTGGCT 
GGAGCCACAG CAGTTTACTC CCAATTCATT ACTCAGCTAA CGGATGAAAA CCAATCCTGT 
TGCCCCGTAT GTCAGAGAAT TTTTCAGACA GAAGCTGAAT TACAAGATGT CATCAGTGAT 
TTGCAGTCTA AGCTGCGGCT TGCTCCAGAT AAACTCAAGT CAACAGAATC AGAACTAAAG 
AAAAAAGAAA AGCGGCGTGA TGAAATGCTG GGGCTTGTGC CCATGAGGCA AGGCATAATT 
GATTTGAAAG AGAAGGAAAT ACCAGAATTA AGAAACAAAC TGCAGAATGT CAATAGAGAC 
ATGCAGCGCC TAAAGAATGA CATAGAAGAA CAGGAAACAC TCTTGGGTAC AATCATGCCT 
GAAGAAGAAA GTGCTAAACA GAGTCTGACA GATGTTACAA TTATGGAGAG GTTGCAGATG 
GAACTTAAAG ATGTTGAAAG AAAAATTGCA CAACAAGCAG CTAAGCTACA AGGACTGGAC 
TTGGATAGAA CCGTTCAACA AGTAAACCAG GAAAAACAAG AAAAACAACA CAAATTAGAT 
ACAGTTTCCA GTAAGATTGA ATTGAATCGT AAGCTTATAC AGGACCAGCA GGAACAGATT 
CAACACCTCA AAAGTATAAC AAATGAACTT AAATCAGAGA AACTTCAGAT ATCCACTAAT 
TTACAGCGTC GTCAGCAGCT GGAGGAACAG ACTGTGGAAT TCTCCACTGA AGTTCAGTCC 
TTATACAGAG AGATTAAGGA TGCTAAAGAG CAGATAAGCC CTTTGGAAAC AACATTGGAA 
AAGTTCCAAC AGGAAAAAGA AGAATTAGTC TACAAAAAAA ATACAAGCAA CAAAATAACA 
CAGGATAAAA TAAATGATAT CAAAGAGAAG GTTAAAAATA TTCATGGTTA TATGAAAAAC 
ATTGAGAATT ATATTCAGGA TGGGAAAGAC GACTATAAGA AGCAAAAAGA AACTGAACTT 
GATAAAGTAA TAGCTCAACT AAGTGAATGC GAGAAACGCA AAGAAAAGAT AAATAAGGAA 
ATGGGAATCA TGAGACAAGA TATTGATACA CAGAAGATAC AAGAAAGATG GCTGCAGGAT 
AACCTAACAT TAAGAAAAAG AAATGAAGAA CTAAAAGAAG TTGAAGAAGA AAGAAAACAA 
CATTTGAAGG AAATGGGTCA AATGCAAGTT TTACAAATGA AAAATGACCA TCAAAAGTTG 
GAAGAAAAAA TAGAAAATAT AAAAAGAAAT CACAGTTTGG CAATAGGGCG ACAGAAGGGT 
TATGAAGAAG AAATTATTCA TTTTAAGAAA GAGCTTCGAG ACTCTCAATT TCGAGATGCT 
GAGGAAAAGT ATAGAGAAAT GATGATTGTT ATGAGAACAA CAGAGCTTGT AAACAAGGAT 
CTGGACATTT ATTATAAGAC CCTTGACCAA GCAATAATGA AATTTCACAG TATGAAAATG 
GAAGAAATCA ATAAAATCAT TCGCGACCTT TGGCGAAGTA CCTATCGCGG ACAAGATATC 
GAATATATAG AAATTCGCTC TGATGCCGAT GAAAACATAG CAGCTTCTGA TAAAAGGCGG 
AATTACAACT ACAGAGTAGT GATGCTAAAA GGAGACACAG CCTTGGATAT GCGAGGACGA 
TGCAGTGCTG GACAAAAGGT ATTAGCATCG CTCATTATTC GCCTGGCCCT GGCTGAAACG 
TTCTGCTTAA ACTGTGGCAT TCTTGCCTTG GATGAGCCAA CAACAAATCT GGACCGAGAA 
AACATTGAAT CTCTTGCACA TGCTCTGGTT GAGATAATAA AAAGTCGCTC ACAGCAACGT 
AACTTCCAGC TTCTGGTAAT CACTCATGAC GAAGATTTTG TGGAGCTCTT AGGGCGGTCT 
GAATATGTGG AAAAATTCTA CAGGATTAAG AAGAACATTG ATCAGTGCTC AGAGATTGTG 
AAATGCAATG TCAACTCCCT GGTATCTTAC GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011360585.1
CDS	312..4250
Translation	MSRIEKMSILGVRSFGIEDKDKQIITFFSPLTILVGPNGAGKTTIIECLKYICTGDFPPGTKGNTFVHDPKIAQETDVRAQIRLQFRDVNGELVAVQRSMVCTQKSKKTEFKTLEGVITRTKHGEKVSLSSKCAEIDREMISSLGVSKSVLNNVIFCHQEDSNWPLSEGKALKQKFDEIFSATRYIKALETIRQVRQTQGQKVKECQTELKYLKQNKEKACEIRNQIASKEDQLTSSKEIVKAYENELDPLKNRLKEIEQNLAKIMKLDNEIKALESRKKQMEKDNSELEQKMEKVFQGTDEQLNDLYHNHQRTVREKERKLVDCQRELEKLNKESRLLNQEKSELLVEQGRLQLQADRHQENIRARDSLIQSLATQLELDGFERGPFSERQIKNFHKLVRERQEKEAETANQLMNDFAEKETLKQKQIDEIRDKKIGLGRIIELKSEILSKKQNELKNVKYELQQLEGSSDRILELDQELTKAERELSKAEKNSNIETLKTEVISLQNEKADLDKTLRKLDQEMEQLNHHTTTRTQMEMLTKDKADKDEQIRKIKSRHSDELNSLLGYFPNKKQLEDWLHSKSKEINQTRDRLAKLNKELASAEQNKNHMNNELKRKEEQLSSYEDKLFDVCGSQDFESDLDRLKEEIEKSSKQRAMLAGATAVYSQFITQLTDENQSCCPVCQRIFQTEAELQDVISDLQSKLRLAPDKLKSTESELKKKEKRRDEMLGLVPMRQGIIDLKEKEIPELRNKLQNVNRDMQRLKNDIEEQETLLGTIMPEEESAKQSLTDVTIMERLQMELKDVERKIAQQAAKLQGLDLDRTVQQVNQEKQEKQHKLDTVSSKIELNRKLIQDQQEQIQHLKSITNELKSEKLQISTNLQRRQQLEEQTVEFSTEVQSLYREIKDAKEQISPLETTLEKFQQEKEELVYKKNTSNKITQDKINDIKEKVKNIHGYMKNIENYIQDGKDDYKKQKETELDKVIAQLSECEKRKEKINKEMGIMRQDIDTQKIQERWLQDNLTLRKRNEELKEVEEERKQHLKEMGQMQVLQMKNDHQKLEEKIENIKRNHSLAIGRQKGYEEEIIHFKKELRDSQFRDAEEKYREMMIVMRTTELVNKDLDIYYKTLDQAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADENIAASDKRRNYNYRVVMLKGDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSQQRNFQLLVITHDEDFVELLGRSEYVEKFYRIKKNIDQCSEIVKCNVNSLVSYVH

Target ORF information:

RefSeq Version	XM_011362283.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus RAD50 homolog (S. cerevisiae) (RAD50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011362283.1

ORF Insert Sequence:

ATGTCCCGGA TCGAAAAGAT GAGCATTCTG GGCGTGCGGA GTTTTGGAAT AGAGGACAAA 
GATAAGCAAA TTATCACTTT CTTCAGTCCC CTCACGATTT TGGTTGGACC CAATGGGGCG 
GGGAAGACGA CCATCATTGA ATGTCTAAAA TATATTTGCA CTGGAGATTT CCCTCCTGGA 
ACCAAAGGGA ATACATTTGT TCATGATCCC AAGATTGCTC AAGAAACAGA TGTTAGAGCC 
CAGATTCGTC TACAGTTTCG TGATGTCAAT GGAGAACTTG TAGCTGTGCA GAGATCTATG 
GTGTGTACTC AGAAAAGCAA AAAGACAGAG TTTAAAACCC TGGAAGGAGT CATTACTAGA 
ACAAAGCATG GTGAAAAGGT CAGTCTCAGC TCTAAGTGTG CAGAAATTGA CCGAGAGATG 
ATAAGTTCTC TTGGGGTTTC CAAGTCTGTG CTAAATAACG TCATTTTCTG TCATCAAGAA 
GATTCTAACT GGCCTTTAAG TGAAGGAAAG GCTTTGAAGC AAAAATTTGA TGAGATTTTT 
TCAGCAACAA GGTACATTAA AGCTTTGGAA ACAATCCGGC AGGTACGTCA GACACAAGGT 
CAGAAAGTAA AAGAATGTCA AACAGAGCTA AAATATCTGA AGCAAAATAA AGAAAAAGCT 
TGTGAGATTC GCAATCAGAT TGCTAGTAAA GAAGACCAGT TAACATCTTC AAAGGAAATT 
GTCAAAGCCT ATGAGAATGA ACTTGATCCA TTGAAGAATC GTCTAAAAGA AATTGAACAA 
AATCTCGCTA AAATAATGAA ACTTGACAAT GAAATTAAAG CCTTGGAAAG CCGAAAGAAG 
CAAATGGAGA AAGATAATAG TGAACTAGAA CAGAAAATGG AAAAGGTTTT TCAAGGGACT 
GATGAGCAAC TAAATGATTT ATATCACAAT CACCAGAGAA CAGTAAGAGA GAAAGAAAGG 
AAATTGGTAG ACTGTCAGCG TGAACTGGAA AAATTAAATA AAGAATCTAG GCTTCTCAAT 
CAAGAAAAGT CAGAACTACT TGTTGAACAG GGTCGTCTAC AGCTACAGGC AGATCGCCAT 
CAAGAAAATA TCCGAGCTAG AGATTCATTA ATTCAATCTT TGGCAACACA GCTAGAATTG 
GATGGCTTTG AGCGTGGACC ATTCAGTGAA AGACAGATTA AAAATTTTCA CAAACTTGTG 
AGAGAGAGAC AAGAAAAAGA AGCAGAAACT GCCAACCAAC TGATGAATGA CTTTGCAGAA 
AAAGAGACTC TGAAACAAAA ACAGATAGAT GAAATAAGGG ATAAGAAAAT TGGACTGGGA 
AGAATAATTG AACTAAAATC AGAAATCCTA AGTAAGAAGC AGAACGAACT GAAAAATGTG 
AAGTACGAAT TACAGCAATT GGAAGGATCT TCAGACAGGA TTCTTGAACT GGACCAGGAG 
CTCACAAAAG CTGAACGTGA GCTAAGCAAG GCTGAGAAAA ATAGCAATAT AGAAACTCTA 
AAAACAGAAG TAATAAGCCT TCAAAATGAG AAAGCAGACC TAGACAAGAC CCTGCGTAAA 
TTGGACCAGG AGATGGAGCA GTTAAACCAT CATACAACAA CGCGCACCCA GATGGAGATG 
TTGACCAAAG ACAAAGCTGA CAAAGATGAA CAAATCAGAA AAATAAAATC TAGGCACAGT 
GATGAATTAA ATTCATTGTT AGGATATTTT CCCAACAAAA AACAGCTTGA AGACTGGCTT 
CATAGTAAAT CAAAAGAAAT TAATCAGACC AGAGACAGAC TTGCCAAATT GAACAAGGAA 
CTAGCTTCAG CTGAGCAAAA TAAAAATCAT ATGAATAATG AGCTAAAAAG GAAGGAAGAG 
CAGTTGTCCA GTTATGAAGA CAAGCTTTTT GATGTTTGTG GTAGCCAGGA TTTTGAAAGT 
GATTTAGACA GACTTAAAGA AGAAATTGAA AAATCCTCAA AACAACGAGC CATGCTGGCT 
GGAGCCACAG CAGTTTACTC CCAATTCATT ACTCAGCTAA CGGATGAAAA CCAATCCTGT 
TGCCCCGTAT GTCAGAGAAT TTTTCAGACA GAAGCTGAAT TACAAGATGT CATCAGTGAT 
TTGCAGTCTA AGCTGCGGCT TGCTCCAGAT AAACTCAAGT CAACAGAATC AGAACTAAAG 
AAAAAAGAAA AGCGGCGTGA TGAAATGCTG GGGCTTGTGC CCATGAGGCA AGGCATAATT 
GATTTGAAAG AGAAGGAAAT ACCAGAATTA AGAAACAAAC TGCAGAATGT CAATAGAGAC 
ATGCAGCGCC TAAAGAATGA CATAGAAGAA CAGGAAACAC TCTTGGGTAC AATCATGCCT 
GAAGAAGAAA GTGCTAAACA GAGTCTGACA GATGTTACAA TTATGGAGAG GTTGCAGATG 
GAACTTAAAG ATGTTGAAAG AAAAATTGCA CAACAAGCAG CTAAGCTACA AGGACTGGAC 
TTGGATAGAA CCGTTCAACA AGTAAACCAG GAAAAACAAG AAAAACAACA CAAATTAGAT 
ACAGTTTCCA GTAAGATTGA ATTGAATCGT AAGCTTATAC AGGACCAGCA GGAACAGATT 
CAACACCTCA AAAGTATAAC AAATGAACTT AAATCAGAGA AACTTCAGAT ATCCACTAAT 
TTACAGCGTC GTCAGCAGCT GGAGGAACAG ACTGTGGAAT TCTCCACTGA AGTTCAGTCC 
TTATACAGAG AGATTAAGGA TGCTAAAGAG CAGATAAGCC CTTTGGAAAC AACATTGGAA 
AAGTTCCAAC AGGAAAAAGA AGAATTAGTC TACAAAAAAA ATACAAGCAA CAAAATAACA 
CAGGATAAAA TAAATGATAT CAAAGAGAAG GTTAAAAATA TTCATGGTTA TATGAAAAAC 
ATTGAGAATT ATATTCAGGA TGGGAAAGAC GACTATAAGA AGCAAAAAGA AACTGAACTT 
GATAAAGTAA TAGCTCAACT AAGTGAATGC GAGAAACGCA AAGAAAAGAT AAATAAGGAA 
ATGGGAATCA TGAGACAAGA TATTGATACA CAGAAGATAC AAGAAAGATG GCTGCAGGAT 
AACCTAACAT TAAGAAAAAG AAATGAAGAA CTAAAAGAAG TTGAAGAAGA AAGAAAACAA 
CATTTGAAGG AAATGGGTCA AATGCAAGTT TTACAAATGA AAAATGACCA TCAAAAGTTG 
GAAGAAAAAA TAGAAAATAT AAAAAGAAAT CACAGTTTGG CAATAGGGCG ACAGAAGGGT 
TATGAAGAAG AAATTATTCA TTTTAAGAAA GAGCTTCGAG ACTCTCAATT TCGAGATGCT 
GAGGAAAAGT ATAGAGAAAT GATGATTGTT ATGAGAACAA CAGAGCTTGT AAACAAGGAT 
CTGGACATTT ATTATAAGAC CCTTGACCAA GCAATAATGA AATTTCACAG TATGAAAATG 
GAAGAAATCA ATAAAATCAT TCGCGACCTT TGGCGAAGTA CCTATCGCGG ACAAGATATC 
GAATATATAG AAATTCGCTC TGATGCCGAT GAAAACATAG CAGCTTCTGA TAAAAGGCGG 
AATTACAACT ACAGAGTAGT GATGCTAAAA GGAGACACAG CCTTGGATAT GCGAGGACGA 
TGCAGTGCTG GACAAAAGGT ATTAGCATCG CTCATTATTC GCCTGGCCCT GGCTGAAACG 
TTCTGCTTAA ACTGTGGCAT TCTTGCCTTG GATGAGCCAA CAACAAATCT GGACCGAGAA 
AACATTGAAT CTCTTGCACA TGCTCTGGTT GAGATAATAA AAAGTCGCTC ACAGCAACGT 
AACTTCCAGC TTCTGGTAAT CACTCATGAC GAAGATTTTG TGGAGCTCTT AGGGCGGTCT 
GAATATGTGG AAAAATTCTA CAGGATTAAG AAGAACATTG ATCAGTGCTC AGAGATTGTG 
AAATGCAATG TCAACTCCCT GGTATCTTAC GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD50 ( XM_011362283.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011360585.1 Pteropus vampyrus RAD50 double strand break repair protein (RAD50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPt48335
Clone ID Related Accession (Same CDS sequence)	XM_011362283.1 , XM_011362283.2
Accession Version	XM_011362283.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	DNA repair protein RAD50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888855.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011362283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGA TCGAAAAGAT GAGCATTCTG GGCGTGCGGA GTTTTGGAAT AGAGGACAAA 
GATAAGCAAA TTATCACTTT CTTCAGTCCC CTCACGATTT TGGTTGGACC CAATGGGGCG 
GGGAAGACGA CCATCATTGA ATGTCTAAAA TATATTTGCA CTGGAGATTT CCCTCCTGGA 
ACCAAAGGGA ATACATTTGT TCATGATCCC AAGATTGCTC AAGAAACAGA TGTTAGAGCC 
CAGATTCGTC TACAGTTTCG TGATGTCAAT GGAGAACTTG TAGCTGTGCA GAGATCTATG 
GTGTGTACTC AGAAAAGCAA AAAGACAGAG TTTAAAACCC TGGAAGGAGT CATTACTAGA 
ACAAAGCATG GTGAAAAGGT CAGTCTCAGC TCTAAGTGTG CAGAAATTGA CCGAGAGATG 
ATAAGTTCTC TTGGGGTTTC CAAGTCTGTG CTAAATAACG TCATTTTCTG TCATCAAGAA 
GATTCTAACT GGCCTTTAAG TGAAGGAAAG GCTTTGAAGC AAAAATTTGA TGAGATTTTT 
TCAGCAACAA GGTACATTAA AGCTTTGGAA ACAATCCGGC AGGTACGTCA GACACAAGGT 
CAGAAAGTAA AAGAATGTCA AACAGAGCTA AAATATCTGA AGCAAAATAA AGAAAAAGCT 
TGTGAGATTC GCAATCAGAT TGCTAGTAAA GAAGACCAGT TAACATCTTC AAAGGAAATT 
GTCAAAGCCT ATGAGAATGA ACTTGATCCA TTGAAGAATC GTCTAAAAGA AATTGAACAA 
AATCTCGCTA AAATAATGAA ACTTGACAAT GAAATTAAAG CCTTGGAAAG CCGAAAGAAG 
CAAATGGAGA AAGATAATAG TGAACTAGAA CAGAAAATGG AAAAGGTTTT TCAAGGGACT 
GATGAGCAAC TAAATGATTT ATATCACAAT CACCAGAGAA CAGTAAGAGA GAAAGAAAGG 
AAATTGGTAG ACTGTCAGCG TGAACTGGAA AAATTAAATA AAGAATCTAG GCTTCTCAAT 
CAAGAAAAGT CAGAACTACT TGTTGAACAG GGTCGTCTAC AGCTACAGGC AGATCGCCAT 
CAAGAAAATA TCCGAGCTAG AGATTCATTA ATTCAATCTT TGGCAACACA GCTAGAATTG 
GATGGCTTTG AGCGTGGACC ATTCAGTGAA AGACAGATTA AAAATTTTCA CAAACTTGTG 
AGAGAGAGAC AAGAAAAAGA AGCAGAAACT GCCAACCAAC TGATGAATGA CTTTGCAGAA 
AAAGAGACTC TGAAACAAAA ACAGATAGAT GAAATAAGGG ATAAGAAAAT TGGACTGGGA 
AGAATAATTG AACTAAAATC AGAAATCCTA AGTAAGAAGC AGAACGAACT GAAAAATGTG 
AAGTACGAAT TACAGCAATT GGAAGGATCT TCAGACAGGA TTCTTGAACT GGACCAGGAG 
CTCACAAAAG CTGAACGTGA GCTAAGCAAG GCTGAGAAAA ATAGCAATAT AGAAACTCTA 
AAAACAGAAG TAATAAGCCT TCAAAATGAG AAAGCAGACC TAGACAAGAC CCTGCGTAAA 
TTGGACCAGG AGATGGAGCA GTTAAACCAT CATACAACAA CGCGCACCCA GATGGAGATG 
TTGACCAAAG ACAAAGCTGA CAAAGATGAA CAAATCAGAA AAATAAAATC TAGGCACAGT 
GATGAATTAA ATTCATTGTT AGGATATTTT CCCAACAAAA AACAGCTTGA AGACTGGCTT 
CATAGTAAAT CAAAAGAAAT TAATCAGACC AGAGACAGAC TTGCCAAATT GAACAAGGAA 
CTAGCTTCAG CTGAGCAAAA TAAAAATCAT ATGAATAATG AGCTAAAAAG GAAGGAAGAG 
CAGTTGTCCA GTTATGAAGA CAAGCTTTTT GATGTTTGTG GTAGCCAGGA TTTTGAAAGT 
GATTTAGACA GACTTAAAGA AGAAATTGAA AAATCCTCAA AACAACGAGC CATGCTGGCT 
GGAGCCACAG CAGTTTACTC CCAATTCATT ACTCAGCTAA CGGATGAAAA CCAATCCTGT 
TGCCCCGTAT GTCAGAGAAT TTTTCAGACA GAAGCTGAAT TACAAGATGT CATCAGTGAT 
TTGCAGTCTA AGCTGCGGCT TGCTCCAGAT AAACTCAAGT CAACAGAATC AGAACTAAAG 
AAAAAAGAAA AGCGGCGTGA TGAAATGCTG GGGCTTGTGC CCATGAGGCA AGGCATAATT 
GATTTGAAAG AGAAGGAAAT ACCAGAATTA AGAAACAAAC TGCAGAATGT CAATAGAGAC 
ATGCAGCGCC TAAAGAATGA CATAGAAGAA CAGGAAACAC TCTTGGGTAC AATCATGCCT 
GAAGAAGAAA GTGCTAAACA GAGTCTGACA GATGTTACAA TTATGGAGAG GTTGCAGATG 
GAACTTAAAG ATGTTGAAAG AAAAATTGCA CAACAAGCAG CTAAGCTACA AGGACTGGAC 
TTGGATAGAA CCGTTCAACA AGTAAACCAG GAAAAACAAG AAAAACAACA CAAATTAGAT 
ACAGTTTCCA GTAAGATTGA ATTGAATCGT AAGCTTATAC AGGACCAGCA GGAACAGATT 
CAACACCTCA AAAGTATAAC AAATGAACTT AAATCAGAGA AACTTCAGAT ATCCACTAAT 
TTACAGCGTC GTCAGCAGCT GGAGGAACAG ACTGTGGAAT TCTCCACTGA AGTTCAGTCC 
TTATACAGAG AGATTAAGGA TGCTAAAGAG CAGATAAGCC CTTTGGAAAC AACATTGGAA 
AAGTTCCAAC AGGAAAAAGA AGAATTAGTC TACAAAAAAA ATACAAGCAA CAAAATAACA 
CAGGATAAAA TAAATGATAT CAAAGAGAAG GTTAAAAATA TTCATGGTTA TATGAAAAAC 
ATTGAGAATT ATATTCAGGA TGGGAAAGAC GACTATAAGA AGCAAAAAGA AACTGAACTT 
GATAAAGTAA TAGCTCAACT AAGTGAATGC GAGAAACGCA AAGAAAAGAT AAATAAGGAA 
ATGGGAATCA TGAGACAAGA TATTGATACA CAGAAGATAC AAGAAAGATG GCTGCAGGAT 
AACCTAACAT TAAGAAAAAG AAATGAAGAA CTAAAAGAAG TTGAAGAAGA AAGAAAACAA 
CATTTGAAGG AAATGGGTCA AATGCAAGTT TTACAAATGA AAAATGACCA TCAAAAGTTG 
GAAGAAAAAA TAGAAAATAT AAAAAGAAAT CACAGTTTGG CAATAGGGCG ACAGAAGGGT 
TATGAAGAAG AAATTATTCA TTTTAAGAAA GAGCTTCGAG ACTCTCAATT TCGAGATGCT 
GAGGAAAAGT ATAGAGAAAT GATGATTGTT ATGAGAACAA CAGAGCTTGT AAACAAGGAT 
CTGGACATTT ATTATAAGAC CCTTGACCAA GCAATAATGA AATTTCACAG TATGAAAATG 
GAAGAAATCA ATAAAATCAT TCGCGACCTT TGGCGAAGTA CCTATCGCGG ACAAGATATC 
GAATATATAG AAATTCGCTC TGATGCCGAT GAAAACATAG CAGCTTCTGA TAAAAGGCGG 
AATTACAACT ACAGAGTAGT GATGCTAAAA GGAGACACAG CCTTGGATAT GCGAGGACGA 
TGCAGTGCTG GACAAAAGGT ATTAGCATCG CTCATTATTC GCCTGGCCCT GGCTGAAACG 
TTCTGCTTAA ACTGTGGCAT TCTTGCCTTG GATGAGCCAA CAACAAATCT GGACCGAGAA 
AACATTGAAT CTCTTGCACA TGCTCTGGTT GAGATAATAA AAAGTCGCTC ACAGCAACGT 
AACTTCCAGC TTCTGGTAAT CACTCATGAC GAAGATTTTG TGGAGCTCTT AGGGCGGTCT 
GAATATGTGG AAAAATTCTA CAGGATTAAG AAGAACATTG ATCAGTGCTC AGAGATTGTG 
AAATGCAATG TCAACTCCCT GGTATCTTAC GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011360585.1
CDS	324..4262
Translation	MSRIEKMSILGVRSFGIEDKDKQIITFFSPLTILVGPNGAGKTTIIECLKYICTGDFPPGTKGNTFVHDPKIAQETDVRAQIRLQFRDVNGELVAVQRSMVCTQKSKKTEFKTLEGVITRTKHGEKVSLSSKCAEIDREMISSLGVSKSVLNNVIFCHQEDSNWPLSEGKALKQKFDEIFSATRYIKALETIRQVRQTQGQKVKECQTELKYLKQNKEKACEIRNQIASKEDQLTSSKEIVKAYENELDPLKNRLKEIEQNLAKIMKLDNEIKALESRKKQMEKDNSELEQKMEKVFQGTDEQLNDLYHNHQRTVREKERKLVDCQRELEKLNKESRLLNQEKSELLVEQGRLQLQADRHQENIRARDSLIQSLATQLELDGFERGPFSERQIKNFHKLVRERQEKEAETANQLMNDFAEKETLKQKQIDEIRDKKIGLGRIIELKSEILSKKQNELKNVKYELQQLEGSSDRILELDQELTKAERELSKAEKNSNIETLKTEVISLQNEKADLDKTLRKLDQEMEQLNHHTTTRTQMEMLTKDKADKDEQIRKIKSRHSDELNSLLGYFPNKKQLEDWLHSKSKEINQTRDRLAKLNKELASAEQNKNHMNNELKRKEEQLSSYEDKLFDVCGSQDFESDLDRLKEEIEKSSKQRAMLAGATAVYSQFITQLTDENQSCCPVCQRIFQTEAELQDVISDLQSKLRLAPDKLKSTESELKKKEKRRDEMLGLVPMRQGIIDLKEKEIPELRNKLQNVNRDMQRLKNDIEEQETLLGTIMPEEESAKQSLTDVTIMERLQMELKDVERKIAQQAAKLQGLDLDRTVQQVNQEKQEKQHKLDTVSSKIELNRKLIQDQQEQIQHLKSITNELKSEKLQISTNLQRRQQLEEQTVEFSTEVQSLYREIKDAKEQISPLETTLEKFQQEKEELVYKKNTSNKITQDKINDIKEKVKNIHGYMKNIENYIQDGKDDYKKQKETELDKVIAQLSECEKRKEKINKEMGIMRQDIDTQKIQERWLQDNLTLRKRNEELKEVEEERKQHLKEMGQMQVLQMKNDHQKLEEKIENIKRNHSLAIGRQKGYEEEIIHFKKELRDSQFRDAEEKYREMMIVMRTTELVNKDLDIYYKTLDQAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADENIAASDKRRNYNYRVVMLKGDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSQQRNFQLLVITHDEDFVELLGRSEYVEKFYRIKKNIDQCSEIVKCNVNSLVSYVH

Target ORF information:

RefSeq Version	XM_011362283.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus RAD50 double strand break repair protein (RAD50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011362283.2

ORF Insert Sequence:

ATGTCCCGGA TCGAAAAGAT GAGCATTCTG GGCGTGCGGA GTTTTGGAAT AGAGGACAAA 
GATAAGCAAA TTATCACTTT CTTCAGTCCC CTCACGATTT TGGTTGGACC CAATGGGGCG 
GGGAAGACGA CCATCATTGA ATGTCTAAAA TATATTTGCA CTGGAGATTT CCCTCCTGGA 
ACCAAAGGGA ATACATTTGT TCATGATCCC AAGATTGCTC AAGAAACAGA TGTTAGAGCC 
CAGATTCGTC TACAGTTTCG TGATGTCAAT GGAGAACTTG TAGCTGTGCA GAGATCTATG 
GTGTGTACTC AGAAAAGCAA AAAGACAGAG TTTAAAACCC TGGAAGGAGT CATTACTAGA 
ACAAAGCATG GTGAAAAGGT CAGTCTCAGC TCTAAGTGTG CAGAAATTGA CCGAGAGATG 
ATAAGTTCTC TTGGGGTTTC CAAGTCTGTG CTAAATAACG TCATTTTCTG TCATCAAGAA 
GATTCTAACT GGCCTTTAAG TGAAGGAAAG GCTTTGAAGC AAAAATTTGA TGAGATTTTT 
TCAGCAACAA GGTACATTAA AGCTTTGGAA ACAATCCGGC AGGTACGTCA GACACAAGGT 
CAGAAAGTAA AAGAATGTCA AACAGAGCTA AAATATCTGA AGCAAAATAA AGAAAAAGCT 
TGTGAGATTC GCAATCAGAT TGCTAGTAAA GAAGACCAGT TAACATCTTC AAAGGAAATT 
GTCAAAGCCT ATGAGAATGA ACTTGATCCA TTGAAGAATC GTCTAAAAGA AATTGAACAA 
AATCTCGCTA AAATAATGAA ACTTGACAAT GAAATTAAAG CCTTGGAAAG CCGAAAGAAG 
CAAATGGAGA AAGATAATAG TGAACTAGAA CAGAAAATGG AAAAGGTTTT TCAAGGGACT 
GATGAGCAAC TAAATGATTT ATATCACAAT CACCAGAGAA CAGTAAGAGA GAAAGAAAGG 
AAATTGGTAG ACTGTCAGCG TGAACTGGAA AAATTAAATA AAGAATCTAG GCTTCTCAAT 
CAAGAAAAGT CAGAACTACT TGTTGAACAG GGTCGTCTAC AGCTACAGGC AGATCGCCAT 
CAAGAAAATA TCCGAGCTAG AGATTCATTA ATTCAATCTT TGGCAACACA GCTAGAATTG 
GATGGCTTTG AGCGTGGACC ATTCAGTGAA AGACAGATTA AAAATTTTCA CAAACTTGTG 
AGAGAGAGAC AAGAAAAAGA AGCAGAAACT GCCAACCAAC TGATGAATGA CTTTGCAGAA 
AAAGAGACTC TGAAACAAAA ACAGATAGAT GAAATAAGGG ATAAGAAAAT TGGACTGGGA 
AGAATAATTG AACTAAAATC AGAAATCCTA AGTAAGAAGC AGAACGAACT GAAAAATGTG 
AAGTACGAAT TACAGCAATT GGAAGGATCT TCAGACAGGA TTCTTGAACT GGACCAGGAG 
CTCACAAAAG CTGAACGTGA GCTAAGCAAG GCTGAGAAAA ATAGCAATAT AGAAACTCTA 
AAAACAGAAG TAATAAGCCT TCAAAATGAG AAAGCAGACC TAGACAAGAC CCTGCGTAAA 
TTGGACCAGG AGATGGAGCA GTTAAACCAT CATACAACAA CGCGCACCCA GATGGAGATG 
TTGACCAAAG ACAAAGCTGA CAAAGATGAA CAAATCAGAA AAATAAAATC TAGGCACAGT 
GATGAATTAA ATTCATTGTT AGGATATTTT CCCAACAAAA AACAGCTTGA AGACTGGCTT 
CATAGTAAAT CAAAAGAAAT TAATCAGACC AGAGACAGAC TTGCCAAATT GAACAAGGAA 
CTAGCTTCAG CTGAGCAAAA TAAAAATCAT ATGAATAATG AGCTAAAAAG GAAGGAAGAG 
CAGTTGTCCA GTTATGAAGA CAAGCTTTTT GATGTTTGTG GTAGCCAGGA TTTTGAAAGT 
GATTTAGACA GACTTAAAGA AGAAATTGAA AAATCCTCAA AACAACGAGC CATGCTGGCT 
GGAGCCACAG CAGTTTACTC CCAATTCATT ACTCAGCTAA CGGATGAAAA CCAATCCTGT 
TGCCCCGTAT GTCAGAGAAT TTTTCAGACA GAAGCTGAAT TACAAGATGT CATCAGTGAT 
TTGCAGTCTA AGCTGCGGCT TGCTCCAGAT AAACTCAAGT CAACAGAATC AGAACTAAAG 
AAAAAAGAAA AGCGGCGTGA TGAAATGCTG GGGCTTGTGC CCATGAGGCA AGGCATAATT 
GATTTGAAAG AGAAGGAAAT ACCAGAATTA AGAAACAAAC TGCAGAATGT CAATAGAGAC 
ATGCAGCGCC TAAAGAATGA CATAGAAGAA CAGGAAACAC TCTTGGGTAC AATCATGCCT 
GAAGAAGAAA GTGCTAAACA GAGTCTGACA GATGTTACAA TTATGGAGAG GTTGCAGATG 
GAACTTAAAG ATGTTGAAAG AAAAATTGCA CAACAAGCAG CTAAGCTACA AGGACTGGAC 
TTGGATAGAA CCGTTCAACA AGTAAACCAG GAAAAACAAG AAAAACAACA CAAATTAGAT 
ACAGTTTCCA GTAAGATTGA ATTGAATCGT AAGCTTATAC AGGACCAGCA GGAACAGATT 
CAACACCTCA AAAGTATAAC AAATGAACTT AAATCAGAGA AACTTCAGAT ATCCACTAAT 
TTACAGCGTC GTCAGCAGCT GGAGGAACAG ACTGTGGAAT TCTCCACTGA AGTTCAGTCC 
TTATACAGAG AGATTAAGGA TGCTAAAGAG CAGATAAGCC CTTTGGAAAC AACATTGGAA 
AAGTTCCAAC AGGAAAAAGA AGAATTAGTC TACAAAAAAA ATACAAGCAA CAAAATAACA 
CAGGATAAAA TAAATGATAT CAAAGAGAAG GTTAAAAATA TTCATGGTTA TATGAAAAAC 
ATTGAGAATT ATATTCAGGA TGGGAAAGAC GACTATAAGA AGCAAAAAGA AACTGAACTT 
GATAAAGTAA TAGCTCAACT AAGTGAATGC GAGAAACGCA AAGAAAAGAT AAATAAGGAA 
ATGGGAATCA TGAGACAAGA TATTGATACA CAGAAGATAC AAGAAAGATG GCTGCAGGAT 
AACCTAACAT TAAGAAAAAG AAATGAAGAA CTAAAAGAAG TTGAAGAAGA AAGAAAACAA 
CATTTGAAGG AAATGGGTCA AATGCAAGTT TTACAAATGA AAAATGACCA TCAAAAGTTG 
GAAGAAAAAA TAGAAAATAT AAAAAGAAAT CACAGTTTGG CAATAGGGCG ACAGAAGGGT 
TATGAAGAAG AAATTATTCA TTTTAAGAAA GAGCTTCGAG ACTCTCAATT TCGAGATGCT 
GAGGAAAAGT ATAGAGAAAT GATGATTGTT ATGAGAACAA CAGAGCTTGT AAACAAGGAT 
CTGGACATTT ATTATAAGAC CCTTGACCAA GCAATAATGA AATTTCACAG TATGAAAATG 
GAAGAAATCA ATAAAATCAT TCGCGACCTT TGGCGAAGTA CCTATCGCGG ACAAGATATC 
GAATATATAG AAATTCGCTC TGATGCCGAT GAAAACATAG CAGCTTCTGA TAAAAGGCGG 
AATTACAACT ACAGAGTAGT GATGCTAAAA GGAGACACAG CCTTGGATAT GCGAGGACGA 
TGCAGTGCTG GACAAAAGGT ATTAGCATCG CTCATTATTC GCCTGGCCCT GGCTGAAACG 
TTCTGCTTAA ACTGTGGCAT TCTTGCCTTG GATGAGCCAA CAACAAATCT GGACCGAGAA 
AACATTGAAT CTCTTGCACA TGCTCTGGTT GAGATAATAA AAAGTCGCTC ACAGCAACGT 
AACTTCCAGC TTCTGGTAAT CACTCATGAC GAAGATTTTG TGGAGCTCTT AGGGCGGTCT 
GAATATGTGG AAAAATTCTA CAGGATTAAG AAGAACATTG ATCAGTGCTC AGAGATTGTG 
AAATGCAATG TCAACTCCCT GGTATCTTAC GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD50 ( XM_023531903.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023387671.1 Pteropus vampyrus RAD50 double strand break repair protein (RAD50), transcript variant X2, mRNA.

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CloneID	OPt70258
Clone ID Related Accession (Same CDS sequence)	XM_023531903.1
Accession Version	XM_023531903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	DNA repair protein RAD50 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888855.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTGTA CTCAGAAAAG CAAAAAGACA GAGTTTAAAA CCCTGGAAGG AGTCATTACT 
AGAACAAAGC ATGGTGAAAA GGTCAGTCTC AGCTCTAAGT GTGCAGAAAT TGACCGAGAG 
ATGATAAGTT CTCTTGGGGT TTCCAAGTCT GTGCTAAATA ACGTCATTTT CTGTCATCAA 
GAAGATTCTA ACTGGCCTTT AAGTGAAGGA AAGGCTTTGA AGCAAAAATT TGATGAGATT 
TTTTCAGCAA CAAGGTACAT TAAAGCTTTG GAAACAATCC GGCAGGTACG TCAGACACAA 
GGTCAGAAAG TAAAAGAATG TCAAACAGAG CTAAAATATC TGAAGCAAAA TAAAGAAAAA 
GCTTGTGAGA TTCGCAATCA GATTGCTAGT AAAGAAGACC AGTTAACATC TTCAAAGGAA 
ATTGTCAAAG CCTATGAGAA TGAACTTGAT CCATTGAAGA ATCGTCTAAA AGAAATTGAA 
CAAAATCTCG CTAAAATAAT GAAACTTGAC AATGAAATTA AAGCCTTGGA AAGCCGAAAG 
AAGCAAATGG AGAAAGATAA TAGTGAACTA GAACAGAAAA TGGAAAAGGT TTTTCAAGGG 
ACTGATGAGC AACTAAATGA TTTATATCAC AATCACCAGA GAACAGTAAG AGAGAAAGAA 
AGGAAATTGG TAGACTGTCA GCGTGAACTG GAAAAATTAA ATAAAGAATC TAGGCTTCTC 
AATCAAGAAA AGTCAGAACT ACTTGTTGAA CAGGGTCGTC TACAGCTACA GGCAGATCGC 
CATCAAGAAA ATATCCGAGC TAGAGATTCA TTAATTCAAT CTTTGGCAAC ACAGCTAGAA 
TTGGATGGCT TTGAGCGTGG ACCATTCAGT GAAAGACAGA TTAAAAATTT TCACAAACTT 
GTGAGAGAGA GACAAGAAAA AGAAGCAGAA ACTGCCAACC AACTGATGAA TGACTTTGCA 
GAAAAAGAGA CTCTGAAACA AAAACAGATA GATGAAATAA GGGATAAGAA AATTGGACTG 
GGAAGAATAA TTGAACTAAA ATCAGAAATC CTAAGTAAGA AGCAGAACGA ACTGAAAAAT 
GTGAAGTACG AATTACAGCA ATTGGAAGGA TCTTCAGACA GGATTCTTGA ACTGGACCAG 
GAGCTCACAA AAGCTGAACG TGAGCTAAGC AAGGCTGAGA AAAATAGCAA TATAGAAACT 
CTAAAAACAG AAGTAATAAG CCTTCAAAAT GAGAAAGCAG ACCTAGACAA GACCCTGCGT 
AAATTGGACC AGGAGATGGA GCAGTTAAAC CATCATACAA CAACGCGCAC CCAGATGGAG 
ATGTTGACCA AAGACAAAGC TGACAAAGAT GAACAAATCA GAAAAATAAA ATCTAGGCAC 
AGTGATGAAT TAAATTCATT GTTAGGATAT TTTCCCAACA AAAAACAGCT TGAAGACTGG 
CTTCATAGTA AATCAAAAGA AATTAATCAG ACCAGAGACA GACTTGCCAA ATTGAACAAG 
GAACTAGCTT CAGCTGAGCA AAATAAAAAT CATATGAATA ATGAGCTAAA AAGGAAGGAA 
GAGCAGTTGT CCAGTTATGA AGACAAGCTT TTTGATGTTT GTGGTAGCCA GGATTTTGAA 
AGTGATTTAG ACAGACTTAA AGAAGAAATT GAAAAATCCT CAAAACAACG AGCCATGCTG 
GCTGGAGCCA CAGCAGTTTA CTCCCAATTC ATTACTCAGC TAACGGATGA AAACCAATCC 
TGTTGCCCCG TATGTCAGAG AATTTTTCAG ACAGAAGCTG AATTACAAGA TGTCATCAGT 
GATTTGCAGT CTAAGCTGCG GCTTGCTCCA GATAAACTCA AGTCAACAGA ATCAGAACTA 
AAGAAAAAAG AAAAGCGGCG TGATGAAATG CTGGGGCTTG TGCCCATGAG GCAAGGCATA 
ATTGATTTGA AAGAGAAGGA AATACCAGAA TTAAGAAACA AACTGCAGAA TGTCAATAGA 
GACATGCAGC GCCTAAAGAA TGACATAGAA GAACAGGAAA CACTCTTGGG TACAATCATG 
CCTGAAGAAG AAAGTGCTAA ACAGAGTCTG ACAGATGTTA CAATTATGGA GAGGTTGCAG 
ATGGAACTTA AAGATGTTGA AAGAAAAATT GCACAACAAG CAGCTAAGCT ACAAGGACTG 
GACTTGGATA GAACCGTTCA ACAAGTAAAC CAGGAAAAAC AAGAAAAACA ACACAAATTA 
GATACAGTTT CCAGTAAGAT TGAATTGAAT CGTAAGCTTA TACAGGACCA GCAGGAACAG 
ATTCAACACC TCAAAAGTAT AACAAATGAA CTTAAATCAG AGAAACTTCA GATATCCACT 
AATTTACAGC GTCGTCAGCA GCTGGAGGAA CAGACTGTGG AATTCTCCAC TGAAGTTCAG 
TCCTTATACA GAGAGATTAA GGATGCTAAA GAGCAGATAA GCCCTTTGGA AACAACATTG 
GAAAAGTTCC AACAGGAAAA AGAAGAATTA GTCTACAAAA AAAATACAAG CAACAAAATA 
ACACAGGATA AAATAAATGA TATCAAAGAG AAGGTTAAAA ATATTCATGG TTATATGAAA 
AACATTGAGA ATTATATTCA GGATGGGAAA GACGACTATA AGAAGCAAAA AGAAACTGAA 
CTTGATAAAG TAATAGCTCA ACTAAGTGAA TGCGAGAAAC GCAAAGAAAA GATAAATAAG 
GAAATGGGAA TCATGAGACA AGATATTGAT ACACAGAAGA TACAAGAAAG ATGGCTGCAG 
GATAACCTAA CATTAAGAAA AAGAAATGAA GAACTAAAAG AAGTTGAAGA AGAAAGAAAA 
CAACATTTGA AGGAAATGGG TCAAATGCAA GTTTTACAAA TGAAAAATGA CCATCAAAAG 
TTGGAAGAAA AAATAGAAAA TATAAAAAGA AATCACAGTT TGGCAATAGG GCGACAGAAG 
GGTTATGAAG AAGAAATTAT TCATTTTAAG AAAGAGCTTC GAGACTCTCA ATTTCGAGAT 
GCTGAGGAAA AGTATAGAGA AATGATGATT GTTATGAGAA CAACAGAGCT TGTAAACAAG 
GATCTGGACA TTTATTATAA GACCCTTGAC CAAGCAATAA TGAAATTTCA CAGTATGAAA 
ATGGAAGAAA TCAATAAAAT CATTCGCGAC CTTTGGCGAA GTACCTATCG CGGACAAGAT 
ATCGAATATA TAGAAATTCG CTCTGATGCC GATGAAAACA TAGCAGCTTC TGATAAAAGG 
CGGAATTACA ACTACAGAGT AGTGATGCTA AAAGGAGACA CAGCCTTGGA TATGCGAGGA 
CGATGCAGTG CTGGACAAAA GGTATTAGCA TCGCTCATTA TTCGCCTGGC CCTGGCTGAA 
ACGTTCTGCT TAAACTGTGG CATTCTTGCC TTGGATGAGC CAACAACAAA TCTGGACCGA 
GAAAACATTG AATCTCTTGC ACATGCTCTG GTTGAGATAA TAAAAAGTCG CTCACAGCAA 
CGTAACTTCC AGCTTCTGGT AATCACTCAT GACGAAGATT TTGTGGAGCT CTTAGGGCGG 
TCTGAATATG TGGAAAAATT CTACAGGATT AAGAAGAACA TTGATCAGTG CTCAGAGATT 
GTGAAATGCA ATGTCAACTC CCTGGTATCT TACGTTCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023387671.1
CDS	233..3874
Translation	MVCTQKSKKTEFKTLEGVITRTKHGEKVSLSSKCAEIDREMISSLGVSKSVLNNVIFCHQEDSNWPLSEGKALKQKFDEIFSATRYIKALETIRQVRQTQGQKVKECQTELKYLKQNKEKACEIRNQIASKEDQLTSSKEIVKAYENELDPLKNRLKEIEQNLAKIMKLDNEIKALESRKKQMEKDNSELEQKMEKVFQGTDEQLNDLYHNHQRTVREKERKLVDCQRELEKLNKESRLLNQEKSELLVEQGRLQLQADRHQENIRARDSLIQSLATQLELDGFERGPFSERQIKNFHKLVRERQEKEAETANQLMNDFAEKETLKQKQIDEIRDKKIGLGRIIELKSEILSKKQNELKNVKYELQQLEGSSDRILELDQELTKAERELSKAEKNSNIETLKTEVISLQNEKADLDKTLRKLDQEMEQLNHHTTTRTQMEMLTKDKADKDEQIRKIKSRHSDELNSLLGYFPNKKQLEDWLHSKSKEINQTRDRLAKLNKELASAEQNKNHMNNELKRKEEQLSSYEDKLFDVCGSQDFESDLDRLKEEIEKSSKQRAMLAGATAVYSQFITQLTDENQSCCPVCQRIFQTEAELQDVISDLQSKLRLAPDKLKSTESELKKKEKRRDEMLGLVPMRQGIIDLKEKEIPELRNKLQNVNRDMQRLKNDIEEQETLLGTIMPEEESAKQSLTDVTIMERLQMELKDVERKIAQQAAKLQGLDLDRTVQQVNQEKQEKQHKLDTVSSKIELNRKLIQDQQEQIQHLKSITNELKSEKLQISTNLQRRQQLEEQTVEFSTEVQSLYREIKDAKEQISPLETTLEKFQQEKEELVYKKNTSNKITQDKINDIKEKVKNIHGYMKNIENYIQDGKDDYKKQKETELDKVIAQLSECEKRKEKINKEMGIMRQDIDTQKIQERWLQDNLTLRKRNEELKEVEEERKQHLKEMGQMQVLQMKNDHQKLEEKIENIKRNHSLAIGRQKGYEEEIIHFKKELRDSQFRDAEEKYREMMIVMRTTELVNKDLDIYYKTLDQAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADENIAASDKRRNYNYRVVMLKGDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSQQRNFQLLVITHDEDFVELLGRSEYVEKFYRIKKNIDQCSEIVKCNVNSLVSYVH

Target ORF information:

RefSeq Version	XM_023531903.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus RAD50 double strand break repair protein (RAD50), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023531903.1

ORF Insert Sequence:

ATGGTGTGTA CTCAGAAAAG CAAAAAGACA GAGTTTAAAA CCCTGGAAGG AGTCATTACT 
AGAACAAAGC ATGGTGAAAA GGTCAGTCTC AGCTCTAAGT GTGCAGAAAT TGACCGAGAG 
ATGATAAGTT CTCTTGGGGT TTCCAAGTCT GTGCTAAATA ACGTCATTTT CTGTCATCAA 
GAAGATTCTA ACTGGCCTTT AAGTGAAGGA AAGGCTTTGA AGCAAAAATT TGATGAGATT 
TTTTCAGCAA CAAGGTACAT TAAAGCTTTG GAAACAATCC GGCAGGTACG TCAGACACAA 
GGTCAGAAAG TAAAAGAATG TCAAACAGAG CTAAAATATC TGAAGCAAAA TAAAGAAAAA 
GCTTGTGAGA TTCGCAATCA GATTGCTAGT AAAGAAGACC AGTTAACATC TTCAAAGGAA 
ATTGTCAAAG CCTATGAGAA TGAACTTGAT CCATTGAAGA ATCGTCTAAA AGAAATTGAA 
CAAAATCTCG CTAAAATAAT GAAACTTGAC AATGAAATTA AAGCCTTGGA AAGCCGAAAG 
AAGCAAATGG AGAAAGATAA TAGTGAACTA GAACAGAAAA TGGAAAAGGT TTTTCAAGGG 
ACTGATGAGC AACTAAATGA TTTATATCAC AATCACCAGA GAACAGTAAG AGAGAAAGAA 
AGGAAATTGG TAGACTGTCA GCGTGAACTG GAAAAATTAA ATAAAGAATC TAGGCTTCTC 
AATCAAGAAA AGTCAGAACT ACTTGTTGAA CAGGGTCGTC TACAGCTACA GGCAGATCGC 
CATCAAGAAA ATATCCGAGC TAGAGATTCA TTAATTCAAT CTTTGGCAAC ACAGCTAGAA 
TTGGATGGCT TTGAGCGTGG ACCATTCAGT GAAAGACAGA TTAAAAATTT TCACAAACTT 
GTGAGAGAGA GACAAGAAAA AGAAGCAGAA ACTGCCAACC AACTGATGAA TGACTTTGCA 
GAAAAAGAGA CTCTGAAACA AAAACAGATA GATGAAATAA GGGATAAGAA AATTGGACTG 
GGAAGAATAA TTGAACTAAA ATCAGAAATC CTAAGTAAGA AGCAGAACGA ACTGAAAAAT 
GTGAAGTACG AATTACAGCA ATTGGAAGGA TCTTCAGACA GGATTCTTGA ACTGGACCAG 
GAGCTCACAA AAGCTGAACG TGAGCTAAGC AAGGCTGAGA AAAATAGCAA TATAGAAACT 
CTAAAAACAG AAGTAATAAG CCTTCAAAAT GAGAAAGCAG ACCTAGACAA GACCCTGCGT 
AAATTGGACC AGGAGATGGA GCAGTTAAAC CATCATACAA CAACGCGCAC CCAGATGGAG 
ATGTTGACCA AAGACAAAGC TGACAAAGAT GAACAAATCA GAAAAATAAA ATCTAGGCAC 
AGTGATGAAT TAAATTCATT GTTAGGATAT TTTCCCAACA AAAAACAGCT TGAAGACTGG 
CTTCATAGTA AATCAAAAGA AATTAATCAG ACCAGAGACA GACTTGCCAA ATTGAACAAG 
GAACTAGCTT CAGCTGAGCA AAATAAAAAT CATATGAATA ATGAGCTAAA AAGGAAGGAA 
GAGCAGTTGT CCAGTTATGA AGACAAGCTT TTTGATGTTT GTGGTAGCCA GGATTTTGAA 
AGTGATTTAG ACAGACTTAA AGAAGAAATT GAAAAATCCT CAAAACAACG AGCCATGCTG 
GCTGGAGCCA CAGCAGTTTA CTCCCAATTC ATTACTCAGC TAACGGATGA AAACCAATCC 
TGTTGCCCCG TATGTCAGAG AATTTTTCAG ACAGAAGCTG AATTACAAGA TGTCATCAGT 
GATTTGCAGT CTAAGCTGCG GCTTGCTCCA GATAAACTCA AGTCAACAGA ATCAGAACTA 
AAGAAAAAAG AAAAGCGGCG TGATGAAATG CTGGGGCTTG TGCCCATGAG GCAAGGCATA 
ATTGATTTGA AAGAGAAGGA AATACCAGAA TTAAGAAACA AACTGCAGAA TGTCAATAGA 
GACATGCAGC GCCTAAAGAA TGACATAGAA GAACAGGAAA CACTCTTGGG TACAATCATG 
CCTGAAGAAG AAAGTGCTAA ACAGAGTCTG ACAGATGTTA CAATTATGGA GAGGTTGCAG 
ATGGAACTTA AAGATGTTGA AAGAAAAATT GCACAACAAG CAGCTAAGCT ACAAGGACTG 
GACTTGGATA GAACCGTTCA ACAAGTAAAC CAGGAAAAAC AAGAAAAACA ACACAAATTA 
GATACAGTTT CCAGTAAGAT TGAATTGAAT CGTAAGCTTA TACAGGACCA GCAGGAACAG 
ATTCAACACC TCAAAAGTAT AACAAATGAA CTTAAATCAG AGAAACTTCA GATATCCACT 
AATTTACAGC GTCGTCAGCA GCTGGAGGAA CAGACTGTGG AATTCTCCAC TGAAGTTCAG 
TCCTTATACA GAGAGATTAA GGATGCTAAA GAGCAGATAA GCCCTTTGGA AACAACATTG 
GAAAAGTTCC AACAGGAAAA AGAAGAATTA GTCTACAAAA AAAATACAAG CAACAAAATA 
ACACAGGATA AAATAAATGA TATCAAAGAG AAGGTTAAAA ATATTCATGG TTATATGAAA 
AACATTGAGA ATTATATTCA GGATGGGAAA GACGACTATA AGAAGCAAAA AGAAACTGAA 
CTTGATAAAG TAATAGCTCA ACTAAGTGAA TGCGAGAAAC GCAAAGAAAA GATAAATAAG 
GAAATGGGAA TCATGAGACA AGATATTGAT ACACAGAAGA TACAAGAAAG ATGGCTGCAG 
GATAACCTAA CATTAAGAAA AAGAAATGAA GAACTAAAAG AAGTTGAAGA AGAAAGAAAA 
CAACATTTGA AGGAAATGGG TCAAATGCAA GTTTTACAAA TGAAAAATGA CCATCAAAAG 
TTGGAAGAAA AAATAGAAAA TATAAAAAGA AATCACAGTT TGGCAATAGG GCGACAGAAG 
GGTTATGAAG AAGAAATTAT TCATTTTAAG AAAGAGCTTC GAGACTCTCA ATTTCGAGAT 
GCTGAGGAAA AGTATAGAGA AATGATGATT GTTATGAGAA CAACAGAGCT TGTAAACAAG 
GATCTGGACA TTTATTATAA GACCCTTGAC CAAGCAATAA TGAAATTTCA CAGTATGAAA 
ATGGAAGAAA TCAATAAAAT CATTCGCGAC CTTTGGCGAA GTACCTATCG CGGACAAGAT 
ATCGAATATA TAGAAATTCG CTCTGATGCC GATGAAAACA TAGCAGCTTC TGATAAAAGG 
CGGAATTACA ACTACAGAGT AGTGATGCTA AAAGGAGACA CAGCCTTGGA TATGCGAGGA 
CGATGCAGTG CTGGACAAAA GGTATTAGCA TCGCTCATTA TTCGCCTGGC CCTGGCTGAA 
ACGTTCTGCT TAAACTGTGG CATTCTTGCC TTGGATGAGC CAACAACAAA TCTGGACCGA 
GAAAACATTG AATCTCTTGC ACATGCTCTG GTTGAGATAA TAAAAAGTCG CTCACAGCAA 
CGTAACTTCC AGCTTCTGGT AATCACTCAT GACGAAGATT TTGTGGAGCT CTTAGGGCGG 
TCTGAATATG TGGAAAAATT CTACAGGATT AAGAAGAACA TTGATCAGTG CTCAGAGATT 
GTGAAATGCA ATGTCAACTC CCTGGTATCT TACGTTCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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RAD50 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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