NEK1 gene cDNA ORF clone, Pteropus vampyrus(large flying fox)

mRNA	Protein	Name
XM_011360277.1	XP_011358579.1	serine/threonine-protein kinase Nek1 isoform X1
XM_011360279.1	XP_011358581.1	serine/threonine-protein kinase Nek1 isoform X3
XM_011360281.2	XP_011358583.1	serine/threonine-protein kinase Nek1 isoform X4
XM_011360282.1	XP_011358584.1	serine/threonine-protein kinase Nek1 isoform X5
XM_023530729.1	XP_023386497.1	serine/threonine-protein kinase Nek1 isoform X1
XM_011360278.2	XP_011358580.1	serine/threonine-protein kinase Nek1 isoform X2
XM_011360282.2	XP_011358584.1	serine/threonine-protein kinase Nek1 isoform X5
XM_011360276.1	XP_011358578.1	serine/threonine-protein kinase Nek1 isoform X1
XM_011360278.1	XP_011358580.1	serine/threonine-protein kinase Nek1 isoform X2
XM_011360279.2	XP_011358581.1	serine/threonine-protein kinase Nek1 isoform X3
XM_011360281.1	XP_011358583.1	serine/threonine-protein kinase Nek1 isoform X4
XM_011360276.2	XP_011358578.1	serine/threonine-protein kinase Nek1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt46892	XM_011360277.1 Latest version!	Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt46892	XM_011360276.2 Latest version!	Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt46892	XM_023530729.1 Latest version!	Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt46893	XM_011360278.2 Latest version!	Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt46894	XM_011360279.2 Latest version!	Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt46895	XM_011360281.2 Latest version!	Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt46896	XM_011360282.2 Latest version!	Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OPt46896	XM_011360282.1	Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt46893	XM_011360278.1	Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt46894	XM_011360279.1	Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt46895	XM_011360281.1	Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt46892	XM_011360276.1	Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

NEK1 ( XM_011360277.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358579.1 Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPt46892
Clone ID Related Accession (Same CDS sequence)	XM_011360277.1 , XM_023530729.1 , XM_011360276.1 , XM_011360276.2
Accession Version	XM_011360277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888834.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGGT AAATACCCAG GAGTGGGTTT GCTTGATCGT 
ATGGGATTCC TGCAAAACCT GGCAGCTATG TATGGAGGCA GGTCCAGCTC TTCAAGAGGA 
GGGAAGCCAA GAAACAAAGA GGAAGAGGTT TATCTTGCAA GGCTGAGACA AATAAGATTG 
CAGAATTTCA ATGAGCGCCA ACAGATTAGA GCCAAACTTC GTGGTGAAAA GAAAGAAGTG 
GATAATTCTG AAGGACAAGA GGGAAGTGAG GAGACTGACA TGAGGCGTAA AAAAGTTGAA 
GCACTTAAGG CCCAAGCAAG TGCACGTGCT TCTGTATTAA AAGAACAATT GGAACGAAAG 
AGAAAGGAAG CGTATGAGAG AGAAAAGAAA GTATGGGAAG AGCATTTGGT GGCTAAAGGA 
GTAAAGAATT CTGATATGTC TCCTTCAGGA CAGCATGATT CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTCACT TCAGCTTTGA AAGAAGTGGG TATGGACAGT 
TTAACTGGTA CCCAGGAACC CGCAGAAGAA ATGCAAAAGA CTAACAATGC TATTTCGAGT 
AAGCGAGAAA TACTACGTAG ATTAAATCAA AATCTTAAAG CTCAAGAAGA TGAAAAAGGC 
AAGCAGTGTC ACTCTGATAC CTGTGAGATA ATCGTTCACG AAGATGACAA AGGACATGAA 
AAAGAAAAGT CAGTTTCATC TGATCGCAAG AAATGGGAGG CAGGAGGGCA GCTCGTGATT 
CCTCTGGATG AGTCTGCATG GGATGCATCC TTCTCTGCAA CCGACAACCA TACAGTGGGA 
GATGTTATTA AATTAGATTC TGGAGGATCT CCAAGAAGAG TCTGGGGGAA AAGCCCTACA 
GATTCTGTTC TAAAAATACT TGGAGAAGCT GAACTACAAC TTCAGACAGA ACTATTGGGA 
AACACAACCA TTAGAAGTGA TATTTCTTCT GAAGGGGAGA AGTACAAACC CTTAATCACT 
GGAGAAGAAA AAGTAAAATG TATTTCACTT GAAATGAACC TATCAGCTAC TCTCCATTCT 
TCTGCTGAGA CAAGCAGTCC AAAGCTGAGT GAGGGATCTC CACAGATGTC ATTGAAACCA 
GAAGGAAATT TGGAAGAACG TGATGACTTG GAAACAGAAG TTTTACAAGA GTCAAGTGGA 
GCAAACAAAG ATGGTAGCTT GCCTTGCACT GTTATGGATG TGTGGATTAG TGAGATAAAA 
GAAACAAAGG AAACTAAATT GGAAGATAGG ATTACTAGTC AGAAAAATGA AGATTCTGAA 
GATGGATTTC TGAGGAATCT GGAACAACTT AGTGAAGTTC ACATAGAACC TGGAATAGAT 
GATTCTCAGC ACTCTTATAA ATGTGATACA GACAAATCTA TACAACCAGA ACCATTTTTC 
CATAAAGTGG TTCATTCCGA ACACTTGAAT GTAGTCTCTC AAATTCAGTC AATTCTGTGT 
TCACCAGAAG AAGCCTTGCC ACTTCGATCT CGCTCTGATT CACCACCAAA AAATAAAAAC 
AAGAATTCTT TGCTGATTGG ACTTTCAACT GGTCTTTTTG ATGCAAATAA CCCAAAGATG 
CTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACACTGAT AGATGTTCCT 
ACTATAGGAG ATGTACATCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAGCACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTTTTTGAAG AAACTGACAC AGATTTACAG 
GAGCTTCAGG CCTCTATGGA GCAGTTACTT AGGGAACAGC CTGGGGAAGA ATACAGTGAG 
GAGGAAGAAT CAGTCTTAAA GAACAGTGAC ATAGAGCAGA CTGCAAATGG GACAGATCTG 
GCAGATGAGG ATGACAATCC CAGTAGTGAA AGTGCTCTAA ATGAAGAATG GCACTCAGAT 
AACAGCGATG GTGAGATAAC TAGTGAATGT GAATATGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCACCTGGA GCAGGAAATA GGCTTTGAAA AGTTCTTTGA GGTTTATGAG 
AAAATAAAGT CTATTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGCT 
CAGAATATTT TGGGAAATGA ACATCAACAT CTTTATGCCA AGATCCTTCA TTTAGTTATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358579.1
CDS	78..3833
Translation	MDKYVRLQKIGEGSFGKAILVKSTEDGKQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGPQLIPAKRPASGQSLASVVSAQKITKPAAKYGVPVAYKKHGDKKLQEKKPLQKLKQAHQTPLKKVNPGEERGKMFEEGARKRRLGFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKATFFGGGGAVAPSFPSRGQYEHYHAIFDQMQRQRAEDNEAKWKGEIRGRRLPERQKGQLAMERAKQVEEFLQRKQEARQNKARAEGHMGKYPGVGLLDRMGFLQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKEVDNSEGQEGSEETDMRRKKVEALKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPSGQHDSGGSPSKQQMRSVISVTSALKEVGMDSLTGTQEPAEEMQKTNNAISSKREILRRLNQNLKAQEDEKGKQCHSDTCEIIVHEDDKGHEKEKSVSSDRKKWEAGGQLVIPLDESAWDASFSATDNHTVGDVIKLDSGGSPRRVWGKSPTDSVLKILGEAELQLQTELLGNTTIRSDISSEGEKYKPLITGEEKVKCISLEMNLSATLHSSAETSSPKLSEGSPQMSLKPEGNLEERDDLETEVLQESSGANKDGSLPCTVMDVWISEIKETKETKLEDRITSQKNEDSEDGFLRNLEQLSEVHIEPGIDDSQHSYKCDTDKSIQPEPFFHKVVHSEHLNVVSQIQSILCSPEEALPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLIDVPTIGDVHQDNLEIDEIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKSIHEDEDENIEICSTIAQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011360277.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360277.1

ORF Insert Sequence:

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGGT AAATACCCAG GAGTGGGTTT GCTTGATCGT 
ATGGGATTCC TGCAAAACCT GGCAGCTATG TATGGAGGCA GGTCCAGCTC TTCAAGAGGA 
GGGAAGCCAA GAAACAAAGA GGAAGAGGTT TATCTTGCAA GGCTGAGACA AATAAGATTG 
CAGAATTTCA ATGAGCGCCA ACAGATTAGA GCCAAACTTC GTGGTGAAAA GAAAGAAGTG 
GATAATTCTG AAGGACAAGA GGGAAGTGAG GAGACTGACA TGAGGCGTAA AAAAGTTGAA 
GCACTTAAGG CCCAAGCAAG TGCACGTGCT TCTGTATTAA AAGAACAATT GGAACGAAAG 
AGAAAGGAAG CGTATGAGAG AGAAAAGAAA GTATGGGAAG AGCATTTGGT GGCTAAAGGA 
GTAAAGAATT CTGATATGTC TCCTTCAGGA CAGCATGATT CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTCACT TCAGCTTTGA AAGAAGTGGG TATGGACAGT 
TTAACTGGTA CCCAGGAACC CGCAGAAGAA ATGCAAAAGA CTAACAATGC TATTTCGAGT 
AAGCGAGAAA TACTACGTAG ATTAAATCAA AATCTTAAAG CTCAAGAAGA TGAAAAAGGC 
AAGCAGTGTC ACTCTGATAC CTGTGAGATA ATCGTTCACG AAGATGACAA AGGACATGAA 
AAAGAAAAGT CAGTTTCATC TGATCGCAAG AAATGGGAGG CAGGAGGGCA GCTCGTGATT 
CCTCTGGATG AGTCTGCATG GGATGCATCC TTCTCTGCAA CCGACAACCA TACAGTGGGA 
GATGTTATTA AATTAGATTC TGGAGGATCT CCAAGAAGAG TCTGGGGGAA AAGCCCTACA 
GATTCTGTTC TAAAAATACT TGGAGAAGCT GAACTACAAC TTCAGACAGA ACTATTGGGA 
AACACAACCA TTAGAAGTGA TATTTCTTCT GAAGGGGAGA AGTACAAACC CTTAATCACT 
GGAGAAGAAA AAGTAAAATG TATTTCACTT GAAATGAACC TATCAGCTAC TCTCCATTCT 
TCTGCTGAGA CAAGCAGTCC AAAGCTGAGT GAGGGATCTC CACAGATGTC ATTGAAACCA 
GAAGGAAATT TGGAAGAACG TGATGACTTG GAAACAGAAG TTTTACAAGA GTCAAGTGGA 
GCAAACAAAG ATGGTAGCTT GCCTTGCACT GTTATGGATG TGTGGATTAG TGAGATAAAA 
GAAACAAAGG AAACTAAATT GGAAGATAGG ATTACTAGTC AGAAAAATGA AGATTCTGAA 
GATGGATTTC TGAGGAATCT GGAACAACTT AGTGAAGTTC ACATAGAACC TGGAATAGAT 
GATTCTCAGC ACTCTTATAA ATGTGATACA GACAAATCTA TACAACCAGA ACCATTTTTC 
CATAAAGTGG TTCATTCCGA ACACTTGAAT GTAGTCTCTC AAATTCAGTC AATTCTGTGT 
TCACCAGAAG AAGCCTTGCC ACTTCGATCT CGCTCTGATT CACCACCAAA AAATAAAAAC 
AAGAATTCTT TGCTGATTGG ACTTTCAACT GGTCTTTTTG ATGCAAATAA CCCAAAGATG 
CTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACACTGAT AGATGTTCCT 
ACTATAGGAG ATGTACATCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAGCACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTTTTTGAAG AAACTGACAC AGATTTACAG 
GAGCTTCAGG CCTCTATGGA GCAGTTACTT AGGGAACAGC CTGGGGAAGA ATACAGTGAG 
GAGGAAGAAT CAGTCTTAAA GAACAGTGAC ATAGAGCAGA CTGCAAATGG GACAGATCTG 
GCAGATGAGG ATGACAATCC CAGTAGTGAA AGTGCTCTAA ATGAAGAATG GCACTCAGAT 
AACAGCGATG GTGAGATAAC TAGTGAATGT GAATATGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCACCTGGA GCAGGAAATA GGCTTTGAAA AGTTCTTTGA GGTTTATGAG 
AAAATAAAGT CTATTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGCT 
CAGAATATTT TGGGAAATGA ACATCAACAT CTTTATGCCA AGATCCTTCA TTTAGTTATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_023530729.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023386497.1 Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPt46892
Clone ID Related Accession (Same CDS sequence)	XM_011360277.1 , XM_023530729.1 , XM_011360276.1 , XM_011360276.2
Accession Version	XM_023530729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888834.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGGT AAATACCCAG GAGTGGGTTT GCTTGATCGT 
ATGGGATTCC TGCAAAACCT GGCAGCTATG TATGGAGGCA GGTCCAGCTC TTCAAGAGGA 
GGGAAGCCAA GAAACAAAGA GGAAGAGGTT TATCTTGCAA GGCTGAGACA AATAAGATTG 
CAGAATTTCA ATGAGCGCCA ACAGATTAGA GCCAAACTTC GTGGTGAAAA GAAAGAAGTG 
GATAATTCTG AAGGACAAGA GGGAAGTGAG GAGACTGACA TGAGGCGTAA AAAAGTTGAA 
GCACTTAAGG CCCAAGCAAG TGCACGTGCT TCTGTATTAA AAGAACAATT GGAACGAAAG 
AGAAAGGAAG CGTATGAGAG AGAAAAGAAA GTATGGGAAG AGCATTTGGT GGCTAAAGGA 
GTAAAGAATT CTGATATGTC TCCTTCAGGA CAGCATGATT CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTCACT TCAGCTTTGA AAGAAGTGGG TATGGACAGT 
TTAACTGGTA CCCAGGAACC CGCAGAAGAA ATGCAAAAGA CTAACAATGC TATTTCGAGT 
AAGCGAGAAA TACTACGTAG ATTAAATCAA AATCTTAAAG CTCAAGAAGA TGAAAAAGGC 
AAGCAGTGTC ACTCTGATAC CTGTGAGATA ATCGTTCACG AAGATGACAA AGGACATGAA 
AAAGAAAAGT CAGTTTCATC TGATCGCAAG AAATGGGAGG CAGGAGGGCA GCTCGTGATT 
CCTCTGGATG AGTCTGCATG GGATGCATCC TTCTCTGCAA CCGACAACCA TACAGTGGGA 
GATGTTATTA AATTAGATTC TGGAGGATCT CCAAGAAGAG TCTGGGGGAA AAGCCCTACA 
GATTCTGTTC TAAAAATACT TGGAGAAGCT GAACTACAAC TTCAGACAGA ACTATTGGGA 
AACACAACCA TTAGAAGTGA TATTTCTTCT GAAGGGGAGA AGTACAAACC CTTAATCACT 
GGAGAAGAAA AAGTAAAATG TATTTCACTT GAAATGAACC TATCAGCTAC TCTCCATTCT 
TCTGCTGAGA CAAGCAGTCC AAAGCTGAGT GAGGGATCTC CACAGATGTC ATTGAAACCA 
GAAGGAAATT TGGAAGAACG TGATGACTTG GAAACAGAAG TTTTACAAGA GTCAAGTGGA 
GCAAACAAAG ATGGTAGCTT GCCTTGCACT GTTATGGATG TGTGGATTAG TGAGATAAAA 
GAAACAAAGG AAACTAAATT GGAAGATAGG ATTACTAGTC AGAAAAATGA AGATTCTGAA 
GATGGATTTC TGAGGAATCT GGAACAACTT AGTGAAGTTC ACATAGAACC TGGAATAGAT 
GATTCTCAGC ACTCTTATAA ATGTGATACA GACAAATCTA TACAACCAGA ACCATTTTTC 
CATAAAGTGG TTCATTCCGA ACACTTGAAT GTAGTCTCTC AAATTCAGTC AATTCTGTGT 
TCACCAGAAG AAGCCTTGCC ACTTCGATCT CGCTCTGATT CACCACCAAA AAATAAAAAC 
AAGAATTCTT TGCTGATTGG ACTTTCAACT GGTCTTTTTG ATGCAAATAA CCCAAAGATG 
CTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACACTGAT AGATGTTCCT 
ACTATAGGAG ATGTACATCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAGCACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTTTTTGAAG AAACTGACAC AGATTTACAG 
GAGCTTCAGG CCTCTATGGA GCAGTTACTT AGGGAACAGC CTGGGGAAGA ATACAGTGAG 
GAGGAAGAAT CAGTCTTAAA GAACAGTGAC ATAGAGCAGA CTGCAAATGG GACAGATCTG 
GCAGATGAGG ATGACAATCC CAGTAGTGAA AGTGCTCTAA ATGAAGAATG GCACTCAGAT 
AACAGCGATG GTGAGATAAC TAGTGAATGT GAATATGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCACCTGGA GCAGGAAATA GGCTTTGAAA AGTTCTTTGA GGTTTATGAG 
AAAATAAAGT CTATTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGCT 
CAGAATATTT TGGGAAATGA ACATCAACAT CTTTATGCCA AGATCCTTCA TTTAGTTATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023386497.1
CDS	662..4417
Translation	MDKYVRLQKIGEGSFGKAILVKSTEDGKQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGPQLIPAKRPASGQSLASVVSAQKITKPAAKYGVPVAYKKHGDKKLQEKKPLQKLKQAHQTPLKKVNPGEERGKMFEEGARKRRLGFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKATFFGGGGAVAPSFPSRGQYEHYHAIFDQMQRQRAEDNEAKWKGEIRGRRLPERQKGQLAMERAKQVEEFLQRKQEARQNKARAEGHMGKYPGVGLLDRMGFLQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKEVDNSEGQEGSEETDMRRKKVEALKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPSGQHDSGGSPSKQQMRSVISVTSALKEVGMDSLTGTQEPAEEMQKTNNAISSKREILRRLNQNLKAQEDEKGKQCHSDTCEIIVHEDDKGHEKEKSVSSDRKKWEAGGQLVIPLDESAWDASFSATDNHTVGDVIKLDSGGSPRRVWGKSPTDSVLKILGEAELQLQTELLGNTTIRSDISSEGEKYKPLITGEEKVKCISLEMNLSATLHSSAETSSPKLSEGSPQMSLKPEGNLEERDDLETEVLQESSGANKDGSLPCTVMDVWISEIKETKETKLEDRITSQKNEDSEDGFLRNLEQLSEVHIEPGIDDSQHSYKCDTDKSIQPEPFFHKVVHSEHLNVVSQIQSILCSPEEALPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLIDVPTIGDVHQDNLEIDEIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKSIHEDEDENIEICSTIAQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_023530729.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023530729.1

ORF Insert Sequence:

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGGT AAATACCCAG GAGTGGGTTT GCTTGATCGT 
ATGGGATTCC TGCAAAACCT GGCAGCTATG TATGGAGGCA GGTCCAGCTC TTCAAGAGGA 
GGGAAGCCAA GAAACAAAGA GGAAGAGGTT TATCTTGCAA GGCTGAGACA AATAAGATTG 
CAGAATTTCA ATGAGCGCCA ACAGATTAGA GCCAAACTTC GTGGTGAAAA GAAAGAAGTG 
GATAATTCTG AAGGACAAGA GGGAAGTGAG GAGACTGACA TGAGGCGTAA AAAAGTTGAA 
GCACTTAAGG CCCAAGCAAG TGCACGTGCT TCTGTATTAA AAGAACAATT GGAACGAAAG 
AGAAAGGAAG CGTATGAGAG AGAAAAGAAA GTATGGGAAG AGCATTTGGT GGCTAAAGGA 
GTAAAGAATT CTGATATGTC TCCTTCAGGA CAGCATGATT CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTCACT TCAGCTTTGA AAGAAGTGGG TATGGACAGT 
TTAACTGGTA CCCAGGAACC CGCAGAAGAA ATGCAAAAGA CTAACAATGC TATTTCGAGT 
AAGCGAGAAA TACTACGTAG ATTAAATCAA AATCTTAAAG CTCAAGAAGA TGAAAAAGGC 
AAGCAGTGTC ACTCTGATAC CTGTGAGATA ATCGTTCACG AAGATGACAA AGGACATGAA 
AAAGAAAAGT CAGTTTCATC TGATCGCAAG AAATGGGAGG CAGGAGGGCA GCTCGTGATT 
CCTCTGGATG AGTCTGCATG GGATGCATCC TTCTCTGCAA CCGACAACCA TACAGTGGGA 
GATGTTATTA AATTAGATTC TGGAGGATCT CCAAGAAGAG TCTGGGGGAA AAGCCCTACA 
GATTCTGTTC TAAAAATACT TGGAGAAGCT GAACTACAAC TTCAGACAGA ACTATTGGGA 
AACACAACCA TTAGAAGTGA TATTTCTTCT GAAGGGGAGA AGTACAAACC CTTAATCACT 
GGAGAAGAAA AAGTAAAATG TATTTCACTT GAAATGAACC TATCAGCTAC TCTCCATTCT 
TCTGCTGAGA CAAGCAGTCC AAAGCTGAGT GAGGGATCTC CACAGATGTC ATTGAAACCA 
GAAGGAAATT TGGAAGAACG TGATGACTTG GAAACAGAAG TTTTACAAGA GTCAAGTGGA 
GCAAACAAAG ATGGTAGCTT GCCTTGCACT GTTATGGATG TGTGGATTAG TGAGATAAAA 
GAAACAAAGG AAACTAAATT GGAAGATAGG ATTACTAGTC AGAAAAATGA AGATTCTGAA 
GATGGATTTC TGAGGAATCT GGAACAACTT AGTGAAGTTC ACATAGAACC TGGAATAGAT 
GATTCTCAGC ACTCTTATAA ATGTGATACA GACAAATCTA TACAACCAGA ACCATTTTTC 
CATAAAGTGG TTCATTCCGA ACACTTGAAT GTAGTCTCTC AAATTCAGTC AATTCTGTGT 
TCACCAGAAG AAGCCTTGCC ACTTCGATCT CGCTCTGATT CACCACCAAA AAATAAAAAC 
AAGAATTCTT TGCTGATTGG ACTTTCAACT GGTCTTTTTG ATGCAAATAA CCCAAAGATG 
CTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACACTGAT AGATGTTCCT 
ACTATAGGAG ATGTACATCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAGCACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTTTTTGAAG AAACTGACAC AGATTTACAG 
GAGCTTCAGG CCTCTATGGA GCAGTTACTT AGGGAACAGC CTGGGGAAGA ATACAGTGAG 
GAGGAAGAAT CAGTCTTAAA GAACAGTGAC ATAGAGCAGA CTGCAAATGG GACAGATCTG 
GCAGATGAGG ATGACAATCC CAGTAGTGAA AGTGCTCTAA ATGAAGAATG GCACTCAGAT 
AACAGCGATG GTGAGATAAC TAGTGAATGT GAATATGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCACCTGGA GCAGGAAATA GGCTTTGAAA AGTTCTTTGA GGTTTATGAG 
AAAATAAAGT CTATTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGCT 
CAGAATATTT TGGGAAATGA ACATCAACAT CTTTATGCCA AGATCCTTCA TTTAGTTATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011360276.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358578.1 Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.

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CloneID	OPt46892
Clone ID Related Accession (Same CDS sequence)	XM_011360277.1 , XM_023530729.1 , XM_011360276.1 , XM_011360276.2
Accession Version	XM_011360276.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888834.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGGT AAATACCCAG GAGTGGGTTT GCTTGATCGT 
ATGGGATTCC TGCAAAACCT GGCAGCTATG TATGGAGGCA GGTCCAGCTC TTCAAGAGGA 
GGGAAGCCAA GAAACAAAGA GGAAGAGGTT TATCTTGCAA GGCTGAGACA AATAAGATTG 
CAGAATTTCA ATGAGCGCCA ACAGATTAGA GCCAAACTTC GTGGTGAAAA GAAAGAAGTG 
GATAATTCTG AAGGACAAGA GGGAAGTGAG GAGACTGACA TGAGGCGTAA AAAAGTTGAA 
GCACTTAAGG CCCAAGCAAG TGCACGTGCT TCTGTATTAA AAGAACAATT GGAACGAAAG 
AGAAAGGAAG CGTATGAGAG AGAAAAGAAA GTATGGGAAG AGCATTTGGT GGCTAAAGGA 
GTAAAGAATT CTGATATGTC TCCTTCAGGA CAGCATGATT CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTCACT TCAGCTTTGA AAGAAGTGGG TATGGACAGT 
TTAACTGGTA CCCAGGAACC CGCAGAAGAA ATGCAAAAGA CTAACAATGC TATTTCGAGT 
AAGCGAGAAA TACTACGTAG ATTAAATCAA AATCTTAAAG CTCAAGAAGA TGAAAAAGGC 
AAGCAGTGTC ACTCTGATAC CTGTGAGATA ATCGTTCACG AAGATGACAA AGGACATGAA 
AAAGAAAAGT CAGTTTCATC TGATCGCAAG AAATGGGAGG CAGGAGGGCA GCTCGTGATT 
CCTCTGGATG AGTCTGCATG GGATGCATCC TTCTCTGCAA CCGACAACCA TACAGTGGGA 
GATGTTATTA AATTAGATTC TGGAGGATCT CCAAGAAGAG TCTGGGGGAA AAGCCCTACA 
GATTCTGTTC TAAAAATACT TGGAGAAGCT GAACTACAAC TTCAGACAGA ACTATTGGGA 
AACACAACCA TTAGAAGTGA TATTTCTTCT GAAGGGGAGA AGTACAAACC CTTAATCACT 
GGAGAAGAAA AAGTAAAATG TATTTCACTT GAAATGAACC TATCAGCTAC TCTCCATTCT 
TCTGCTGAGA CAAGCAGTCC AAAGCTGAGT GAGGGATCTC CACAGATGTC ATTGAAACCA 
GAAGGAAATT TGGAAGAACG TGATGACTTG GAAACAGAAG TTTTACAAGA GTCAAGTGGA 
GCAAACAAAG ATGGTAGCTT GCCTTGCACT GTTATGGATG TGTGGATTAG TGAGATAAAA 
GAAACAAAGG AAACTAAATT GGAAGATAGG ATTACTAGTC AGAAAAATGA AGATTCTGAA 
GATGGATTTC TGAGGAATCT GGAACAACTT AGTGAAGTTC ACATAGAACC TGGAATAGAT 
GATTCTCAGC ACTCTTATAA ATGTGATACA GACAAATCTA TACAACCAGA ACCATTTTTC 
CATAAAGTGG TTCATTCCGA ACACTTGAAT GTAGTCTCTC AAATTCAGTC AATTCTGTGT 
TCACCAGAAG AAGCCTTGCC ACTTCGATCT CGCTCTGATT CACCACCAAA AAATAAAAAC 
AAGAATTCTT TGCTGATTGG ACTTTCAACT GGTCTTTTTG ATGCAAATAA CCCAAAGATG 
CTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACACTGAT AGATGTTCCT 
ACTATAGGAG ATGTACATCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAGCACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTTTTTGAAG AAACTGACAC AGATTTACAG 
GAGCTTCAGG CCTCTATGGA GCAGTTACTT AGGGAACAGC CTGGGGAAGA ATACAGTGAG 
GAGGAAGAAT CAGTCTTAAA GAACAGTGAC ATAGAGCAGA CTGCAAATGG GACAGATCTG 
GCAGATGAGG ATGACAATCC CAGTAGTGAA AGTGCTCTAA ATGAAGAATG GCACTCAGAT 
AACAGCGATG GTGAGATAAC TAGTGAATGT GAATATGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCACCTGGA GCAGGAAATA GGCTTTGAAA AGTTCTTTGA GGTTTATGAG 
AAAATAAAGT CTATTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGCT 
CAGAATATTT TGGGAAATGA ACATCAACAT CTTTATGCCA AGATCCTTCA TTTAGTTATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358578.1
CDS	590..4345
Translation	MDKYVRLQKIGEGSFGKAILVKSTEDGKQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGPQLIPAKRPASGQSLASVVSAQKITKPAAKYGVPVAYKKHGDKKLQEKKPLQKLKQAHQTPLKKVNPGEERGKMFEEGARKRRLGFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKATFFGGGGAVAPSFPSRGQYEHYHAIFDQMQRQRAEDNEAKWKGEIRGRRLPERQKGQLAMERAKQVEEFLQRKQEARQNKARAEGHMGKYPGVGLLDRMGFLQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKEVDNSEGQEGSEETDMRRKKVEALKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPSGQHDSGGSPSKQQMRSVISVTSALKEVGMDSLTGTQEPAEEMQKTNNAISSKREILRRLNQNLKAQEDEKGKQCHSDTCEIIVHEDDKGHEKEKSVSSDRKKWEAGGQLVIPLDESAWDASFSATDNHTVGDVIKLDSGGSPRRVWGKSPTDSVLKILGEAELQLQTELLGNTTIRSDISSEGEKYKPLITGEEKVKCISLEMNLSATLHSSAETSSPKLSEGSPQMSLKPEGNLEERDDLETEVLQESSGANKDGSLPCTVMDVWISEIKETKETKLEDRITSQKNEDSEDGFLRNLEQLSEVHIEPGIDDSQHSYKCDTDKSIQPEPFFHKVVHSEHLNVVSQIQSILCSPEEALPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLIDVPTIGDVHQDNLEIDEIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKSIHEDEDENIEICSTIAQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011360276.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360276.1

ORF Insert Sequence:

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGGT AAATACCCAG GAGTGGGTTT GCTTGATCGT 
ATGGGATTCC TGCAAAACCT GGCAGCTATG TATGGAGGCA GGTCCAGCTC TTCAAGAGGA 
GGGAAGCCAA GAAACAAAGA GGAAGAGGTT TATCTTGCAA GGCTGAGACA AATAAGATTG 
CAGAATTTCA ATGAGCGCCA ACAGATTAGA GCCAAACTTC GTGGTGAAAA GAAAGAAGTG 
GATAATTCTG AAGGACAAGA GGGAAGTGAG GAGACTGACA TGAGGCGTAA AAAAGTTGAA 
GCACTTAAGG CCCAAGCAAG TGCACGTGCT TCTGTATTAA AAGAACAATT GGAACGAAAG 
AGAAAGGAAG CGTATGAGAG AGAAAAGAAA GTATGGGAAG AGCATTTGGT GGCTAAAGGA 
GTAAAGAATT CTGATATGTC TCCTTCAGGA CAGCATGATT CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTCACT TCAGCTTTGA AAGAAGTGGG TATGGACAGT 
TTAACTGGTA CCCAGGAACC CGCAGAAGAA ATGCAAAAGA CTAACAATGC TATTTCGAGT 
AAGCGAGAAA TACTACGTAG ATTAAATCAA AATCTTAAAG CTCAAGAAGA TGAAAAAGGC 
AAGCAGTGTC ACTCTGATAC CTGTGAGATA ATCGTTCACG AAGATGACAA AGGACATGAA 
AAAGAAAAGT CAGTTTCATC TGATCGCAAG AAATGGGAGG CAGGAGGGCA GCTCGTGATT 
CCTCTGGATG AGTCTGCATG GGATGCATCC TTCTCTGCAA CCGACAACCA TACAGTGGGA 
GATGTTATTA AATTAGATTC TGGAGGATCT CCAAGAAGAG TCTGGGGGAA AAGCCCTACA 
GATTCTGTTC TAAAAATACT TGGAGAAGCT GAACTACAAC TTCAGACAGA ACTATTGGGA 
AACACAACCA TTAGAAGTGA TATTTCTTCT GAAGGGGAGA AGTACAAACC CTTAATCACT 
GGAGAAGAAA AAGTAAAATG TATTTCACTT GAAATGAACC TATCAGCTAC TCTCCATTCT 
TCTGCTGAGA CAAGCAGTCC AAAGCTGAGT GAGGGATCTC CACAGATGTC ATTGAAACCA 
GAAGGAAATT TGGAAGAACG TGATGACTTG GAAACAGAAG TTTTACAAGA GTCAAGTGGA 
GCAAACAAAG ATGGTAGCTT GCCTTGCACT GTTATGGATG TGTGGATTAG TGAGATAAAA 
GAAACAAAGG AAACTAAATT GGAAGATAGG ATTACTAGTC AGAAAAATGA AGATTCTGAA 
GATGGATTTC TGAGGAATCT GGAACAACTT AGTGAAGTTC ACATAGAACC TGGAATAGAT 
GATTCTCAGC ACTCTTATAA ATGTGATACA GACAAATCTA TACAACCAGA ACCATTTTTC 
CATAAAGTGG TTCATTCCGA ACACTTGAAT GTAGTCTCTC AAATTCAGTC AATTCTGTGT 
TCACCAGAAG AAGCCTTGCC ACTTCGATCT CGCTCTGATT CACCACCAAA AAATAAAAAC 
AAGAATTCTT TGCTGATTGG ACTTTCAACT GGTCTTTTTG ATGCAAATAA CCCAAAGATG 
CTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACACTGAT AGATGTTCCT 
ACTATAGGAG ATGTACATCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAGCACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTTTTTGAAG AAACTGACAC AGATTTACAG 
GAGCTTCAGG CCTCTATGGA GCAGTTACTT AGGGAACAGC CTGGGGAAGA ATACAGTGAG 
GAGGAAGAAT CAGTCTTAAA GAACAGTGAC ATAGAGCAGA CTGCAAATGG GACAGATCTG 
GCAGATGAGG ATGACAATCC CAGTAGTGAA AGTGCTCTAA ATGAAGAATG GCACTCAGAT 
AACAGCGATG GTGAGATAAC TAGTGAATGT GAATATGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCACCTGGA GCAGGAAATA GGCTTTGAAA AGTTCTTTGA GGTTTATGAG 
AAAATAAAGT CTATTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGCT 
CAGAATATTT TGGGAAATGA ACATCAACAT CTTTATGCCA AGATCCTTCA TTTAGTTATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011360276.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358578.1 Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPt46892
Clone ID Related Accession (Same CDS sequence)	XM_011360277.1 , XM_023530729.1 , XM_011360276.1 , XM_011360276.2
Accession Version	XM_011360276.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888834.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011360276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGGT AAATACCCAG GAGTGGGTTT GCTTGATCGT 
ATGGGATTCC TGCAAAACCT GGCAGCTATG TATGGAGGCA GGTCCAGCTC TTCAAGAGGA 
GGGAAGCCAA GAAACAAAGA GGAAGAGGTT TATCTTGCAA GGCTGAGACA AATAAGATTG 
CAGAATTTCA ATGAGCGCCA ACAGATTAGA GCCAAACTTC GTGGTGAAAA GAAAGAAGTG 
GATAATTCTG AAGGACAAGA GGGAAGTGAG GAGACTGACA TGAGGCGTAA AAAAGTTGAA 
GCACTTAAGG CCCAAGCAAG TGCACGTGCT TCTGTATTAA AAGAACAATT GGAACGAAAG 
AGAAAGGAAG CGTATGAGAG AGAAAAGAAA GTATGGGAAG AGCATTTGGT GGCTAAAGGA 
GTAAAGAATT CTGATATGTC TCCTTCAGGA CAGCATGATT CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTCACT TCAGCTTTGA AAGAAGTGGG TATGGACAGT 
TTAACTGGTA CCCAGGAACC CGCAGAAGAA ATGCAAAAGA CTAACAATGC TATTTCGAGT 
AAGCGAGAAA TACTACGTAG ATTAAATCAA AATCTTAAAG CTCAAGAAGA TGAAAAAGGC 
AAGCAGTGTC ACTCTGATAC CTGTGAGATA ATCGTTCACG AAGATGACAA AGGACATGAA 
AAAGAAAAGT CAGTTTCATC TGATCGCAAG AAATGGGAGG CAGGAGGGCA GCTCGTGATT 
CCTCTGGATG AGTCTGCATG GGATGCATCC TTCTCTGCAA CCGACAACCA TACAGTGGGA 
GATGTTATTA AATTAGATTC TGGAGGATCT CCAAGAAGAG TCTGGGGGAA AAGCCCTACA 
GATTCTGTTC TAAAAATACT TGGAGAAGCT GAACTACAAC TTCAGACAGA ACTATTGGGA 
AACACAACCA TTAGAAGTGA TATTTCTTCT GAAGGGGAGA AGTACAAACC CTTAATCACT 
GGAGAAGAAA AAGTAAAATG TATTTCACTT GAAATGAACC TATCAGCTAC TCTCCATTCT 
TCTGCTGAGA CAAGCAGTCC AAAGCTGAGT GAGGGATCTC CACAGATGTC ATTGAAACCA 
GAAGGAAATT TGGAAGAACG TGATGACTTG GAAACAGAAG TTTTACAAGA GTCAAGTGGA 
GCAAACAAAG ATGGTAGCTT GCCTTGCACT GTTATGGATG TGTGGATTAG TGAGATAAAA 
GAAACAAAGG AAACTAAATT GGAAGATAGG ATTACTAGTC AGAAAAATGA AGATTCTGAA 
GATGGATTTC TGAGGAATCT GGAACAACTT AGTGAAGTTC ACATAGAACC TGGAATAGAT 
GATTCTCAGC ACTCTTATAA ATGTGATACA GACAAATCTA TACAACCAGA ACCATTTTTC 
CATAAAGTGG TTCATTCCGA ACACTTGAAT GTAGTCTCTC AAATTCAGTC AATTCTGTGT 
TCACCAGAAG AAGCCTTGCC ACTTCGATCT CGCTCTGATT CACCACCAAA AAATAAAAAC 
AAGAATTCTT TGCTGATTGG ACTTTCAACT GGTCTTTTTG ATGCAAATAA CCCAAAGATG 
CTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACACTGAT AGATGTTCCT 
ACTATAGGAG ATGTACATCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAGCACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTTTTTGAAG AAACTGACAC AGATTTACAG 
GAGCTTCAGG CCTCTATGGA GCAGTTACTT AGGGAACAGC CTGGGGAAGA ATACAGTGAG 
GAGGAAGAAT CAGTCTTAAA GAACAGTGAC ATAGAGCAGA CTGCAAATGG GACAGATCTG 
GCAGATGAGG ATGACAATCC CAGTAGTGAA AGTGCTCTAA ATGAAGAATG GCACTCAGAT 
AACAGCGATG GTGAGATAAC TAGTGAATGT GAATATGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCACCTGGA GCAGGAAATA GGCTTTGAAA AGTTCTTTGA GGTTTATGAG 
AAAATAAAGT CTATTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGCT 
CAGAATATTT TGGGAAATGA ACATCAACAT CTTTATGCCA AGATCCTTCA TTTAGTTATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358578.1
CDS	608..4363
Translation	MDKYVRLQKIGEGSFGKAILVKSTEDGKQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGPQLIPAKRPASGQSLASVVSAQKITKPAAKYGVPVAYKKHGDKKLQEKKPLQKLKQAHQTPLKKVNPGEERGKMFEEGARKRRLGFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKATFFGGGGAVAPSFPSRGQYEHYHAIFDQMQRQRAEDNEAKWKGEIRGRRLPERQKGQLAMERAKQVEEFLQRKQEARQNKARAEGHMGKYPGVGLLDRMGFLQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKEVDNSEGQEGSEETDMRRKKVEALKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPSGQHDSGGSPSKQQMRSVISVTSALKEVGMDSLTGTQEPAEEMQKTNNAISSKREILRRLNQNLKAQEDEKGKQCHSDTCEIIVHEDDKGHEKEKSVSSDRKKWEAGGQLVIPLDESAWDASFSATDNHTVGDVIKLDSGGSPRRVWGKSPTDSVLKILGEAELQLQTELLGNTTIRSDISSEGEKYKPLITGEEKVKCISLEMNLSATLHSSAETSSPKLSEGSPQMSLKPEGNLEERDDLETEVLQESSGANKDGSLPCTVMDVWISEIKETKETKLEDRITSQKNEDSEDGFLRNLEQLSEVHIEPGIDDSQHSYKCDTDKSIQPEPFFHKVVHSEHLNVVSQIQSILCSPEEALPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLIDVPTIGDVHQDNLEIDEIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKSIHEDEDENIEICSTIAQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011360276.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360276.2

ORF Insert Sequence:

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGGT AAATACCCAG GAGTGGGTTT GCTTGATCGT 
ATGGGATTCC TGCAAAACCT GGCAGCTATG TATGGAGGCA GGTCCAGCTC TTCAAGAGGA 
GGGAAGCCAA GAAACAAAGA GGAAGAGGTT TATCTTGCAA GGCTGAGACA AATAAGATTG 
CAGAATTTCA ATGAGCGCCA ACAGATTAGA GCCAAACTTC GTGGTGAAAA GAAAGAAGTG 
GATAATTCTG AAGGACAAGA GGGAAGTGAG GAGACTGACA TGAGGCGTAA AAAAGTTGAA 
GCACTTAAGG CCCAAGCAAG TGCACGTGCT TCTGTATTAA AAGAACAATT GGAACGAAAG 
AGAAAGGAAG CGTATGAGAG AGAAAAGAAA GTATGGGAAG AGCATTTGGT GGCTAAAGGA 
GTAAAGAATT CTGATATGTC TCCTTCAGGA CAGCATGATT CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTCACT TCAGCTTTGA AAGAAGTGGG TATGGACAGT 
TTAACTGGTA CCCAGGAACC CGCAGAAGAA ATGCAAAAGA CTAACAATGC TATTTCGAGT 
AAGCGAGAAA TACTACGTAG ATTAAATCAA AATCTTAAAG CTCAAGAAGA TGAAAAAGGC 
AAGCAGTGTC ACTCTGATAC CTGTGAGATA ATCGTTCACG AAGATGACAA AGGACATGAA 
AAAGAAAAGT CAGTTTCATC TGATCGCAAG AAATGGGAGG CAGGAGGGCA GCTCGTGATT 
CCTCTGGATG AGTCTGCATG GGATGCATCC TTCTCTGCAA CCGACAACCA TACAGTGGGA 
GATGTTATTA AATTAGATTC TGGAGGATCT CCAAGAAGAG TCTGGGGGAA AAGCCCTACA 
GATTCTGTTC TAAAAATACT TGGAGAAGCT GAACTACAAC TTCAGACAGA ACTATTGGGA 
AACACAACCA TTAGAAGTGA TATTTCTTCT GAAGGGGAGA AGTACAAACC CTTAATCACT 
GGAGAAGAAA AAGTAAAATG TATTTCACTT GAAATGAACC TATCAGCTAC TCTCCATTCT 
TCTGCTGAGA CAAGCAGTCC AAAGCTGAGT GAGGGATCTC CACAGATGTC ATTGAAACCA 
GAAGGAAATT TGGAAGAACG TGATGACTTG GAAACAGAAG TTTTACAAGA GTCAAGTGGA 
GCAAACAAAG ATGGTAGCTT GCCTTGCACT GTTATGGATG TGTGGATTAG TGAGATAAAA 
GAAACAAAGG AAACTAAATT GGAAGATAGG ATTACTAGTC AGAAAAATGA AGATTCTGAA 
GATGGATTTC TGAGGAATCT GGAACAACTT AGTGAAGTTC ACATAGAACC TGGAATAGAT 
GATTCTCAGC ACTCTTATAA ATGTGATACA GACAAATCTA TACAACCAGA ACCATTTTTC 
CATAAAGTGG TTCATTCCGA ACACTTGAAT GTAGTCTCTC AAATTCAGTC AATTCTGTGT 
TCACCAGAAG AAGCCTTGCC ACTTCGATCT CGCTCTGATT CACCACCAAA AAATAAAAAC 
AAGAATTCTT TGCTGATTGG ACTTTCAACT GGTCTTTTTG ATGCAAATAA CCCAAAGATG 
CTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACACTGAT AGATGTTCCT 
ACTATAGGAG ATGTACATCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAGCACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTTTTTGAAG AAACTGACAC AGATTTACAG 
GAGCTTCAGG CCTCTATGGA GCAGTTACTT AGGGAACAGC CTGGGGAAGA ATACAGTGAG 
GAGGAAGAAT CAGTCTTAAA GAACAGTGAC ATAGAGCAGA CTGCAAATGG GACAGATCTG 
GCAGATGAGG ATGACAATCC CAGTAGTGAA AGTGCTCTAA ATGAAGAATG GCACTCAGAT 
AACAGCGATG GTGAGATAAC TAGTGAATGT GAATATGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCACCTGGA GCAGGAAATA GGCTTTGAAA AGTTCTTTGA GGTTTATGAG 
AAAATAAAGT CTATTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGCT 
CAGAATATTT TGGGAAATGA ACATCAACAT CTTTATGCCA AGATCCTTCA TTTAGTTATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011360278.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358580.1 Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPt46893
Clone ID Related Accession (Same CDS sequence)	XM_011360278.2 , XM_011360278.1
Accession Version	XM_011360278.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888834.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011360278.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGGA TTCCTGCAAA ACCTGGCAGC TATGTATGGA 
GGCAGGTCCA GCTCTTCAAG AGGAGGGAAG CCAAGAAACA AAGAGGAAGA GGTTTATCTT 
GCAAGGCTGA GACAAATAAG ATTGCAGAAT TTCAATGAGC GCCAACAGAT TAGAGCCAAA 
CTTCGTGGTG AAAAGAAAGA AGTGGATAAT TCTGAAGGAC AAGAGGGAAG TGAGGAGACT 
GACATGAGGC GTAAAAAAGT TGAAGCACTT AAGGCCCAAG CAAGTGCACG TGCTTCTGTA 
TTAAAAGAAC AATTGGAACG AAAGAGAAAG GAAGCGTATG AGAGAGAAAA GAAAGTATGG 
GAAGAGCATT TGGTGGCTAA AGGAGTAAAG AATTCTGATA TGTCTCCTTC AGGACAGCAT 
GATTCAGGTG GCTCTCCATC AAAGCAACAG ATGAGATCTG TTATTTCTGT CACTTCAGCT 
TTGAAAGAAG TGGGTATGGA CAGTTTAACT GGTACCCAGG AACCCGCAGA AGAAATGCAA 
AAGACTAACA ATGCTATTTC GAGTAAGCGA GAAATACTAC GTAGATTAAA TCAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGCAAGCAG TGTCACTCTG ATACCTGTGA GATAATCGTT 
CACGAAGATG ACAAAGGACA TGAAAAAGAA AAGTCAGTTT CATCTGATCG CAAGAAATGG 
GAGGCAGGAG GGCAGCTCGT GATTCCTCTG GATGAGTCTG CATGGGATGC ATCCTTCTCT 
GCAACCGACA ACCATACAGT GGGAGATGTT ATTAAATTAG ATTCTGGAGG ATCTCCAAGA 
AGAGTCTGGG GGAAAAGCCC TACAGATTCT GTTCTAAAAA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT GGGAAACACA ACCATTAGAA GTGATATTTC TTCTGAAGGG 
GAGAAGTACA AACCCTTAAT CACTGGAGAA GAAAAAGTAA AATGTATTTC ACTTGAAATG 
AACCTATCAG CTACTCTCCA TTCTTCTGCT GAGACAAGCA GTCCAAAGCT GAGTGAGGGA 
TCTCCACAGA TGTCATTGAA ACCAGAAGGA AATTTGGAAG AACGTGATGA CTTGGAAACA 
GAAGTTTTAC AAGAGTCAAG TGGAGCAAAC AAAGATGGTA GCTTGCCTTG CACTGTTATG 
GATGTGTGGA TTAGTGAGAT AAAAGAAACA AAGGAAACTA AATTGGAAGA TAGGATTACT 
AGTCAGAAAA ATGAAGATTC TGAAGATGGA TTTCTGAGGA ATCTGGAACA ACTTAGTGAA 
GTTCACATAG AACCTGGAAT AGATGATTCT CAGCACTCTT ATAAATGTGA TACAGACAAA 
TCTATACAAC CAGAACCATT TTTCCATAAA GTGGTTCATT CCGAACACTT GAATGTAGTC 
TCTCAAATTC AGTCAATTCT GTGTTCACCA GAAGAAGCCT TGCCACTTCG ATCTCGCTCT 
GATTCACCAC CAAAAAATAA AAACAAGAAT TCTTTGCTGA TTGGACTTTC AACTGGTCTT 
TTTGATGCAA ATAACCCAAA GATGCTAAGG ACATGTTCAC TTCCAGATCT CTCAAAGCTG 
TTCAGAACAC TGATAGATGT TCCTACTATA GGAGATGTAC ATCAAGACAA TCTTGAAATA 
GATGAAATTG AAGATGAGCA CATTAAAGAA GGACCTTCTG ATTCTGAAGA CATTGTTTTT 
GAAGAAACTG ACACAGATTT ACAGGAGCTT CAGGCCTCTA TGGAGCAGTT ACTTAGGGAA 
CAGCCTGGGG AAGAATACAG TGAGGAGGAA GAATCAGTCT TAAAGAACAG TGACATAGAG 
CAGACTGCAA ATGGGACAGA TCTGGCAGAT GAGGATGACA ATCCCAGTAG TGAAAGTGCT 
CTAAATGAAG AATGGCACTC AGATAACAGC GATGGTGAGA TAACTAGTGA ATGTGAATAT 
GATAGTGTCT TTAACCATTT AGAGGAACTG AGACTTCACC TGGAGCAGGA AATAGGCTTT 
GAAAAGTTCT TTGAGGTTTA TGAGAAAATA AAGTCTATTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGCTCAGAAT ATTTTGGGAA ATGAACATCA ACATCTTTAT 
GCCAAGATCC TTCATTTAGT TATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358580.1
CDS	608..4327
Translation	MDKYVRLQKIGEGSFGKAILVKSTEDGKQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGPQLIPAKRPASGQSLASVVSAQKITKPAAKYGVPVAYKKHGDKKLQEKKPLQKLKQAHQTPLKKVNPGEERGKMFEEGARKRRLGFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKATFFGGGGAVAPSFPSRGQYEHYHAIFDQMQRQRAEDNEAKWKGEIRGRRLPERQKGQLAMERAKQVEEFLQRKQEARQNKARAEGHMGFLQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKEVDNSEGQEGSEETDMRRKKVEALKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPSGQHDSGGSPSKQQMRSVISVTSALKEVGMDSLTGTQEPAEEMQKTNNAISSKREILRRLNQNLKAQEDEKGKQCHSDTCEIIVHEDDKGHEKEKSVSSDRKKWEAGGQLVIPLDESAWDASFSATDNHTVGDVIKLDSGGSPRRVWGKSPTDSVLKILGEAELQLQTELLGNTTIRSDISSEGEKYKPLITGEEKVKCISLEMNLSATLHSSAETSSPKLSEGSPQMSLKPEGNLEERDDLETEVLQESSGANKDGSLPCTVMDVWISEIKETKETKLEDRITSQKNEDSEDGFLRNLEQLSEVHIEPGIDDSQHSYKCDTDKSIQPEPFFHKVVHSEHLNVVSQIQSILCSPEEALPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLIDVPTIGDVHQDNLEIDEIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKSIHEDEDENIEICSTIAQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011360278.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360278.2

ORF Insert Sequence:

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGGA TTCCTGCAAA ACCTGGCAGC TATGTATGGA 
GGCAGGTCCA GCTCTTCAAG AGGAGGGAAG CCAAGAAACA AAGAGGAAGA GGTTTATCTT 
GCAAGGCTGA GACAAATAAG ATTGCAGAAT TTCAATGAGC GCCAACAGAT TAGAGCCAAA 
CTTCGTGGTG AAAAGAAAGA AGTGGATAAT TCTGAAGGAC AAGAGGGAAG TGAGGAGACT 
GACATGAGGC GTAAAAAAGT TGAAGCACTT AAGGCCCAAG CAAGTGCACG TGCTTCTGTA 
TTAAAAGAAC AATTGGAACG AAAGAGAAAG GAAGCGTATG AGAGAGAAAA GAAAGTATGG 
GAAGAGCATT TGGTGGCTAA AGGAGTAAAG AATTCTGATA TGTCTCCTTC AGGACAGCAT 
GATTCAGGTG GCTCTCCATC AAAGCAACAG ATGAGATCTG TTATTTCTGT CACTTCAGCT 
TTGAAAGAAG TGGGTATGGA CAGTTTAACT GGTACCCAGG AACCCGCAGA AGAAATGCAA 
AAGACTAACA ATGCTATTTC GAGTAAGCGA GAAATACTAC GTAGATTAAA TCAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGCAAGCAG TGTCACTCTG ATACCTGTGA GATAATCGTT 
CACGAAGATG ACAAAGGACA TGAAAAAGAA AAGTCAGTTT CATCTGATCG CAAGAAATGG 
GAGGCAGGAG GGCAGCTCGT GATTCCTCTG GATGAGTCTG CATGGGATGC ATCCTTCTCT 
GCAACCGACA ACCATACAGT GGGAGATGTT ATTAAATTAG ATTCTGGAGG ATCTCCAAGA 
AGAGTCTGGG GGAAAAGCCC TACAGATTCT GTTCTAAAAA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT GGGAAACACA ACCATTAGAA GTGATATTTC TTCTGAAGGG 
GAGAAGTACA AACCCTTAAT CACTGGAGAA GAAAAAGTAA AATGTATTTC ACTTGAAATG 
AACCTATCAG CTACTCTCCA TTCTTCTGCT GAGACAAGCA GTCCAAAGCT GAGTGAGGGA 
TCTCCACAGA TGTCATTGAA ACCAGAAGGA AATTTGGAAG AACGTGATGA CTTGGAAACA 
GAAGTTTTAC AAGAGTCAAG TGGAGCAAAC AAAGATGGTA GCTTGCCTTG CACTGTTATG 
GATGTGTGGA TTAGTGAGAT AAAAGAAACA AAGGAAACTA AATTGGAAGA TAGGATTACT 
AGTCAGAAAA ATGAAGATTC TGAAGATGGA TTTCTGAGGA ATCTGGAACA ACTTAGTGAA 
GTTCACATAG AACCTGGAAT AGATGATTCT CAGCACTCTT ATAAATGTGA TACAGACAAA 
TCTATACAAC CAGAACCATT TTTCCATAAA GTGGTTCATT CCGAACACTT GAATGTAGTC 
TCTCAAATTC AGTCAATTCT GTGTTCACCA GAAGAAGCCT TGCCACTTCG ATCTCGCTCT 
GATTCACCAC CAAAAAATAA AAACAAGAAT TCTTTGCTGA TTGGACTTTC AACTGGTCTT 
TTTGATGCAA ATAACCCAAA GATGCTAAGG ACATGTTCAC TTCCAGATCT CTCAAAGCTG 
TTCAGAACAC TGATAGATGT TCCTACTATA GGAGATGTAC ATCAAGACAA TCTTGAAATA 
GATGAAATTG AAGATGAGCA CATTAAAGAA GGACCTTCTG ATTCTGAAGA CATTGTTTTT 
GAAGAAACTG ACACAGATTT ACAGGAGCTT CAGGCCTCTA TGGAGCAGTT ACTTAGGGAA 
CAGCCTGGGG AAGAATACAG TGAGGAGGAA GAATCAGTCT TAAAGAACAG TGACATAGAG 
CAGACTGCAA ATGGGACAGA TCTGGCAGAT GAGGATGACA ATCCCAGTAG TGAAAGTGCT 
CTAAATGAAG AATGGCACTC AGATAACAGC GATGGTGAGA TAACTAGTGA ATGTGAATAT 
GATAGTGTCT TTAACCATTT AGAGGAACTG AGACTTCACC TGGAGCAGGA AATAGGCTTT 
GAAAAGTTCT TTGAGGTTTA TGAGAAAATA AAGTCTATTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGCTCAGAAT ATTTTGGGAA ATGAACATCA ACATCTTTAT 
GCCAAGATCC TTCATTTAGT TATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011360278.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358580.1 Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.

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CloneID	OPt46893
Clone ID Related Accession (Same CDS sequence)	XM_011360278.2 , XM_011360278.1
Accession Version	XM_011360278.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888834.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGGA TTCCTGCAAA ACCTGGCAGC TATGTATGGA 
GGCAGGTCCA GCTCTTCAAG AGGAGGGAAG CCAAGAAACA AAGAGGAAGA GGTTTATCTT 
GCAAGGCTGA GACAAATAAG ATTGCAGAAT TTCAATGAGC GCCAACAGAT TAGAGCCAAA 
CTTCGTGGTG AAAAGAAAGA AGTGGATAAT TCTGAAGGAC AAGAGGGAAG TGAGGAGACT 
GACATGAGGC GTAAAAAAGT TGAAGCACTT AAGGCCCAAG CAAGTGCACG TGCTTCTGTA 
TTAAAAGAAC AATTGGAACG AAAGAGAAAG GAAGCGTATG AGAGAGAAAA GAAAGTATGG 
GAAGAGCATT TGGTGGCTAA AGGAGTAAAG AATTCTGATA TGTCTCCTTC AGGACAGCAT 
GATTCAGGTG GCTCTCCATC AAAGCAACAG ATGAGATCTG TTATTTCTGT CACTTCAGCT 
TTGAAAGAAG TGGGTATGGA CAGTTTAACT GGTACCCAGG AACCCGCAGA AGAAATGCAA 
AAGACTAACA ATGCTATTTC GAGTAAGCGA GAAATACTAC GTAGATTAAA TCAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGCAAGCAG TGTCACTCTG ATACCTGTGA GATAATCGTT 
CACGAAGATG ACAAAGGACA TGAAAAAGAA AAGTCAGTTT CATCTGATCG CAAGAAATGG 
GAGGCAGGAG GGCAGCTCGT GATTCCTCTG GATGAGTCTG CATGGGATGC ATCCTTCTCT 
GCAACCGACA ACCATACAGT GGGAGATGTT ATTAAATTAG ATTCTGGAGG ATCTCCAAGA 
AGAGTCTGGG GGAAAAGCCC TACAGATTCT GTTCTAAAAA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT GGGAAACACA ACCATTAGAA GTGATATTTC TTCTGAAGGG 
GAGAAGTACA AACCCTTAAT CACTGGAGAA GAAAAAGTAA AATGTATTTC ACTTGAAATG 
AACCTATCAG CTACTCTCCA TTCTTCTGCT GAGACAAGCA GTCCAAAGCT GAGTGAGGGA 
TCTCCACAGA TGTCATTGAA ACCAGAAGGA AATTTGGAAG AACGTGATGA CTTGGAAACA 
GAAGTTTTAC AAGAGTCAAG TGGAGCAAAC AAAGATGGTA GCTTGCCTTG CACTGTTATG 
GATGTGTGGA TTAGTGAGAT AAAAGAAACA AAGGAAACTA AATTGGAAGA TAGGATTACT 
AGTCAGAAAA ATGAAGATTC TGAAGATGGA TTTCTGAGGA ATCTGGAACA ACTTAGTGAA 
GTTCACATAG AACCTGGAAT AGATGATTCT CAGCACTCTT ATAAATGTGA TACAGACAAA 
TCTATACAAC CAGAACCATT TTTCCATAAA GTGGTTCATT CCGAACACTT GAATGTAGTC 
TCTCAAATTC AGTCAATTCT GTGTTCACCA GAAGAAGCCT TGCCACTTCG ATCTCGCTCT 
GATTCACCAC CAAAAAATAA AAACAAGAAT TCTTTGCTGA TTGGACTTTC AACTGGTCTT 
TTTGATGCAA ATAACCCAAA GATGCTAAGG ACATGTTCAC TTCCAGATCT CTCAAAGCTG 
TTCAGAACAC TGATAGATGT TCCTACTATA GGAGATGTAC ATCAAGACAA TCTTGAAATA 
GATGAAATTG AAGATGAGCA CATTAAAGAA GGACCTTCTG ATTCTGAAGA CATTGTTTTT 
GAAGAAACTG ACACAGATTT ACAGGAGCTT CAGGCCTCTA TGGAGCAGTT ACTTAGGGAA 
CAGCCTGGGG AAGAATACAG TGAGGAGGAA GAATCAGTCT TAAAGAACAG TGACATAGAG 
CAGACTGCAA ATGGGACAGA TCTGGCAGAT GAGGATGACA ATCCCAGTAG TGAAAGTGCT 
CTAAATGAAG AATGGCACTC AGATAACAGC GATGGTGAGA TAACTAGTGA ATGTGAATAT 
GATAGTGTCT TTAACCATTT AGAGGAACTG AGACTTCACC TGGAGCAGGA AATAGGCTTT 
GAAAAGTTCT TTGAGGTTTA TGAGAAAATA AAGTCTATTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGCTCAGAAT ATTTTGGGAA ATGAACATCA ACATCTTTAT 
GCCAAGATCC TTCATTTAGT TATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358580.1
CDS	590..4309
Translation	MDKYVRLQKIGEGSFGKAILVKSTEDGKQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGPQLIPAKRPASGQSLASVVSAQKITKPAAKYGVPVAYKKHGDKKLQEKKPLQKLKQAHQTPLKKVNPGEERGKMFEEGARKRRLGFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKATFFGGGGAVAPSFPSRGQYEHYHAIFDQMQRQRAEDNEAKWKGEIRGRRLPERQKGQLAMERAKQVEEFLQRKQEARQNKARAEGHMGFLQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKEVDNSEGQEGSEETDMRRKKVEALKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPSGQHDSGGSPSKQQMRSVISVTSALKEVGMDSLTGTQEPAEEMQKTNNAISSKREILRRLNQNLKAQEDEKGKQCHSDTCEIIVHEDDKGHEKEKSVSSDRKKWEAGGQLVIPLDESAWDASFSATDNHTVGDVIKLDSGGSPRRVWGKSPTDSVLKILGEAELQLQTELLGNTTIRSDISSEGEKYKPLITGEEKVKCISLEMNLSATLHSSAETSSPKLSEGSPQMSLKPEGNLEERDDLETEVLQESSGANKDGSLPCTVMDVWISEIKETKETKLEDRITSQKNEDSEDGFLRNLEQLSEVHIEPGIDDSQHSYKCDTDKSIQPEPFFHKVVHSEHLNVVSQIQSILCSPEEALPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLIDVPTIGDVHQDNLEIDEIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKSIHEDEDENIEICSTIAQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011360278.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360278.1

ORF Insert Sequence:

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGGA TTCCTGCAAA ACCTGGCAGC TATGTATGGA 
GGCAGGTCCA GCTCTTCAAG AGGAGGGAAG CCAAGAAACA AAGAGGAAGA GGTTTATCTT 
GCAAGGCTGA GACAAATAAG ATTGCAGAAT TTCAATGAGC GCCAACAGAT TAGAGCCAAA 
CTTCGTGGTG AAAAGAAAGA AGTGGATAAT TCTGAAGGAC AAGAGGGAAG TGAGGAGACT 
GACATGAGGC GTAAAAAAGT TGAAGCACTT AAGGCCCAAG CAAGTGCACG TGCTTCTGTA 
TTAAAAGAAC AATTGGAACG AAAGAGAAAG GAAGCGTATG AGAGAGAAAA GAAAGTATGG 
GAAGAGCATT TGGTGGCTAA AGGAGTAAAG AATTCTGATA TGTCTCCTTC AGGACAGCAT 
GATTCAGGTG GCTCTCCATC AAAGCAACAG ATGAGATCTG TTATTTCTGT CACTTCAGCT 
TTGAAAGAAG TGGGTATGGA CAGTTTAACT GGTACCCAGG AACCCGCAGA AGAAATGCAA 
AAGACTAACA ATGCTATTTC GAGTAAGCGA GAAATACTAC GTAGATTAAA TCAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGCAAGCAG TGTCACTCTG ATACCTGTGA GATAATCGTT 
CACGAAGATG ACAAAGGACA TGAAAAAGAA AAGTCAGTTT CATCTGATCG CAAGAAATGG 
GAGGCAGGAG GGCAGCTCGT GATTCCTCTG GATGAGTCTG CATGGGATGC ATCCTTCTCT 
GCAACCGACA ACCATACAGT GGGAGATGTT ATTAAATTAG ATTCTGGAGG ATCTCCAAGA 
AGAGTCTGGG GGAAAAGCCC TACAGATTCT GTTCTAAAAA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT GGGAAACACA ACCATTAGAA GTGATATTTC TTCTGAAGGG 
GAGAAGTACA AACCCTTAAT CACTGGAGAA GAAAAAGTAA AATGTATTTC ACTTGAAATG 
AACCTATCAG CTACTCTCCA TTCTTCTGCT GAGACAAGCA GTCCAAAGCT GAGTGAGGGA 
TCTCCACAGA TGTCATTGAA ACCAGAAGGA AATTTGGAAG AACGTGATGA CTTGGAAACA 
GAAGTTTTAC AAGAGTCAAG TGGAGCAAAC AAAGATGGTA GCTTGCCTTG CACTGTTATG 
GATGTGTGGA TTAGTGAGAT AAAAGAAACA AAGGAAACTA AATTGGAAGA TAGGATTACT 
AGTCAGAAAA ATGAAGATTC TGAAGATGGA TTTCTGAGGA ATCTGGAACA ACTTAGTGAA 
GTTCACATAG AACCTGGAAT AGATGATTCT CAGCACTCTT ATAAATGTGA TACAGACAAA 
TCTATACAAC CAGAACCATT TTTCCATAAA GTGGTTCATT CCGAACACTT GAATGTAGTC 
TCTCAAATTC AGTCAATTCT GTGTTCACCA GAAGAAGCCT TGCCACTTCG ATCTCGCTCT 
GATTCACCAC CAAAAAATAA AAACAAGAAT TCTTTGCTGA TTGGACTTTC AACTGGTCTT 
TTTGATGCAA ATAACCCAAA GATGCTAAGG ACATGTTCAC TTCCAGATCT CTCAAAGCTG 
TTCAGAACAC TGATAGATGT TCCTACTATA GGAGATGTAC ATCAAGACAA TCTTGAAATA 
GATGAAATTG AAGATGAGCA CATTAAAGAA GGACCTTCTG ATTCTGAAGA CATTGTTTTT 
GAAGAAACTG ACACAGATTT ACAGGAGCTT CAGGCCTCTA TGGAGCAGTT ACTTAGGGAA 
CAGCCTGGGG AAGAATACAG TGAGGAGGAA GAATCAGTCT TAAAGAACAG TGACATAGAG 
CAGACTGCAA ATGGGACAGA TCTGGCAGAT GAGGATGACA ATCCCAGTAG TGAAAGTGCT 
CTAAATGAAG AATGGCACTC AGATAACAGC GATGGTGAGA TAACTAGTGA ATGTGAATAT 
GATAGTGTCT TTAACCATTT AGAGGAACTG AGACTTCACC TGGAGCAGGA AATAGGCTTT 
GAAAAGTTCT TTGAGGTTTA TGAGAAAATA AAGTCTATTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGCTCAGAAT ATTTTGGGAA ATGAACATCA ACATCTTTAT 
GCCAAGATCC TTCATTTAGT TATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011360279.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358581.1 Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPt46894
Clone ID Related Accession (Same CDS sequence)	XM_011360279.1 , XM_011360279.2
Accession Version	XM_011360279.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888834.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGAACATATT TTTAACCAAA GATGGGACAA TACAACTTGG AGATTTTGGA 
ATTGCTAGAG TTCTTAATAG TACCGTAGAA CTTGCTCGAA CCTGCATAGG GACCCCATAC 
TACTTGTCAC CTGAAATCTG TGAAAACAAA CCTTACAACA ATAAAAGTGA CATTTGGGCT 
CTTGGATGTG TCCTTTATGA GATGTGCACA CTTAAACATG CATTTGAAGC TGGCAATATG 
AAAAACCTGG TACTGAAGAT AATATCTGGG TCTTTTCCAC CTGTGTCTTT GCATTATTCT 
TATGATCTCC GCAGTTTGCT CTCTCAGTTA TTTAAAAGAA ATCCTAGGGA TAGACCATCC 
GTCAACTCCA TATTGGAGAA AGGTTTTATA GCCAAACGCA TTGAAAAGTT TCTCTCACCT 
CAGCTTATTG CAGAAGAATT TTGTCTAAAA ACATTTTCCA AGTTTGGACC TCAACTTATA 
CCAGCTAAAA GACCAGCTTC AGGACAAAGC CTGGCCTCTG TTGTGTCTGC TCAGAAAATT 
ACAAAGCCTG CTGCTAAATA TGGCGTGCCT GTGGCATATA AGAAACATGG AGATAAAAAA 
TTGCAGGAAA AGAAGCCACT CCAAAAACTT AAGCAGGCCC ATCAAACTCC ATTGAAGAAA 
GTGAATCCTG GAGAAGAAAG GGGGAAAATG TTTGAGGAAG GAGCAAGAAA GAGAAGGTTG 
GGATTTATCG AAAAAGAAAA GAAACAAAAA GATCAGATTA GTTTAATGAA GGCTGAACAG 
ATGAAAAGGC AAGAAAAGGA AAGGTTGGAG AGAATAAATA GGGCCAGGGA ACAAGGATGG 
AGAAATGTGC TAAGTGCTGG TGGAAGTGGT GAAGTAAAGG CTACCTTTTT TGGCGGTGGA 
GGGGCTGTGG CTCCATCATT TCCTTCTCGA GGACAATATG AACATTATCA TGCTATTTTT 
GATCAAATGC AGCGACAAAG AGCAGAAGAT AATGAAGCTA AATGGAAAGG AGAAATACGT 
GGCCGAAGGC TTCCAGAGAG GCAAAAGGGG CAGCTAGCTA TGGAAAGAGC TAAACAAGTG 
GAAGAATTCT TACAGCGAAA ACAGGAAGCG AGGCAGAACA AAGCTCGAGC CGAAGGGCAC 
ATGGGTAAAT ACCCAGGAGT GGGTTTGCTT GATCGTATGG GATTCCTGCA AAACCTGGCA 
GCTATGTATG GAGGCAGGTC CAGCTCTTCA AGAGGAGGGA AGCCAAGAAA CAAAGAGGAA 
GAGGTTTATC TTGCAAGGCT GAGACAAATA AGATTGCAGA ATTTCAATGA GCGCCAACAG 
ATTAGAGCCA AACTTCGTGG TGAAAAGAAA GAAGTGGATA ATTCTGAAGG ACAAGAGGGA 
AGTGAGGAGA CTGACATGAG GCGTAAAAAA GTTGAAGCAC TTAAGGCCCA AGCAAGTGCA 
CGTGCTTCTG TATTAAAAGA ACAATTGGAA CGAAAGAGAA AGGAAGCGTA TGAGAGAGAA 
AAGAAAGTAT GGGAAGAGCA TTTGGTGGCT AAAGGAGTAA AGAATTCTGA TATGTCTCCT 
TCAGGACAGC ATGATTCAGG TGGCTCTCCA TCAAAGCAAC AGATGAGATC TGTTATTTCT 
GTCACTTCAG CTTTGAAAGA AGTGGGTATG GACAGTTTAA CTGGTACCCA GGAACCCGCA 
GAAGAAATGC AAAAGACTAA CAATGCTATT TCGAGTAAGC GAGAAATACT ACGTAGATTA 
AATCAAAATC TTAAAGCTCA AGAAGATGAA AAAGGCAAGC AGTGTCACTC TGATACCTGT 
GAGATAATCG TTCACGAAGA TGACAAAGGA CATGAAAAAG AAAAGTCAGT TTCATCTGAT 
CGCAAGAAAT GGGAGGCAGG AGGGCAGCTC GTGATTCCTC TGGATGAGTC TGCATGGGAT 
GCATCCTTCT CTGCAACCGA CAACCATACA GTGGGAGATG TTATTAAATT AGATTCTGGA 
GGATCTCCAA GAAGAGTCTG GGGGAAAAGC CCTACAGATT CTGTTCTAAA AATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTGGGAAACA CAACCATTAG AAGTGATATT 
TCTTCTGAAG GGGAGAAGTA CAAACCCTTA ATCACTGGAG AAGAAAAAGT AAAATGTATT 
TCACTTGAAA TGAACCTATC AGCTACTCTC CATTCTTCTG CTGAGACAAG CAGTCCAAAG 
CTGAGTGAGG GATCTCCACA GATGTCATTG AAACCAGAAG GAAATTTGGA AGAACGTGAT 
GACTTGGAAA CAGAAGTTTT ACAAGAGTCA AGTGGAGCAA ACAAAGATGG TAGCTTGCCT 
TGCACTGTTA TGGATGTGTG GATTAGTGAG ATAAAAGAAA CAAAGGAAAC TAAATTGGAA 
GATAGGATTA CTAGTCAGAA AAATGAAGAT TCTGAAGATG GATTTCTGAG GAATCTGGAA 
CAACTTAGTG AAGTTCACAT AGAACCTGGA ATAGATGATT CTCAGCACTC TTATAAATGT 
GATACAGACA AATCTATACA ACCAGAACCA TTTTTCCATA AAGTGGTTCA TTCCGAACAC 
TTGAATGTAG TCTCTCAAAT TCAGTCAATT CTGTGTTCAC CAGAAGAAGC CTTGCCACTT 
CGATCTCGCT CTGATTCACC ACCAAAAAAT AAAAACAAGA ATTCTTTGCT GATTGGACTT 
TCAACTGGTC TTTTTGATGC AAATAACCCA AAGATGCTAA GGACATGTTC ACTTCCAGAT 
CTCTCAAAGC TGTTCAGAAC ACTGATAGAT GTTCCTACTA TAGGAGATGT ACATCAAGAC 
AATCTTGAAA TAGATGAAAT TGAAGATGAG CACATTAAAG AAGGACCTTC TGATTCTGAA 
GACATTGTTT TTGAAGAAAC TGACACAGAT TTACAGGAGC TTCAGGCCTC TATGGAGCAG 
TTACTTAGGG AACAGCCTGG GGAAGAATAC AGTGAGGAGG AAGAATCAGT CTTAAAGAAC 
AGTGACATAG AGCAGACTGC AAATGGGACA GATCTGGCAG ATGAGGATGA CAATCCCAGT 
AGTGAAAGTG CTCTAAATGA AGAATGGCAC TCAGATAACA GCGATGGTGA GATAACTAGT 
GAATGTGAAT ATGATAGTGT CTTTAACCAT TTAGAGGAAC TGAGACTTCA CCTGGAGCAG 
GAAATAGGCT TTGAAAAGTT CTTTGAGGTT TATGAGAAAA TAAAGTCTAT TCATGAAGAT 
GAAGATGAAA ATATTGAGAT TTGTTCAACA ATAGCTCAGA ATATTTTGGG AAATGAACAT 
CAACATCTTT ATGCCAAGAT CCTTCATTTA GTTATGGCAG ATGGAGCCTA TCAAGAAGAT 
AATGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358581.1
CDS	590..4261
Translation	MDKYVRLQKIGEGSFGKAILVKSTEDGKQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGPQLIPAKRPASGQSLASVVSAQKITKPAAKYGVPVAYKKHGDKKLQEKKPLQKLKQAHQTPLKKVNPGEERGKMFEEGARKRRLGFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKATFFGGGGAVAPSFPSRGQYEHYHAIFDQMQRQRAEDNEAKWKGEIRGRRLPERQKGQLAMERAKQVEEFLQRKQEARQNKARAEGHMGKYPGVGLLDRMGFLQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKEVDNSEGQEGSEETDMRRKKVEALKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPSGQHDSGGSPSKQQMRSVISVTSALKEVGMDSLTGTQEPAEEMQKTNNAISSKREILRRLNQNLKAQEDEKGKQCHSDTCEIIVHEDDKGHEKEKSVSSDRKKWEAGGQLVIPLDESAWDASFSATDNHTVGDVIKLDSGGSPRRVWGKSPTDSVLKILGEAELQLQTELLGNTTIRSDISSEGEKYKPLITGEEKVKCISLEMNLSATLHSSAETSSPKLSEGSPQMSLKPEGNLEERDDLETEVLQESSGANKDGSLPCTVMDVWISEIKETKETKLEDRITSQKNEDSEDGFLRNLEQLSEVHIEPGIDDSQHSYKCDTDKSIQPEPFFHKVVHSEHLNVVSQIQSILCSPEEALPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLIDVPTIGDVHQDNLEIDEIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKSIHEDEDENIEICSTIAQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011360279.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360279.1

ORF Insert Sequence:

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGAACATATT TTTAACCAAA GATGGGACAA TACAACTTGG AGATTTTGGA 
ATTGCTAGAG TTCTTAATAG TACCGTAGAA CTTGCTCGAA CCTGCATAGG GACCCCATAC 
TACTTGTCAC CTGAAATCTG TGAAAACAAA CCTTACAACA ATAAAAGTGA CATTTGGGCT 
CTTGGATGTG TCCTTTATGA GATGTGCACA CTTAAACATG CATTTGAAGC TGGCAATATG 
AAAAACCTGG TACTGAAGAT AATATCTGGG TCTTTTCCAC CTGTGTCTTT GCATTATTCT 
TATGATCTCC GCAGTTTGCT CTCTCAGTTA TTTAAAAGAA ATCCTAGGGA TAGACCATCC 
GTCAACTCCA TATTGGAGAA AGGTTTTATA GCCAAACGCA TTGAAAAGTT TCTCTCACCT 
CAGCTTATTG CAGAAGAATT TTGTCTAAAA ACATTTTCCA AGTTTGGACC TCAACTTATA 
CCAGCTAAAA GACCAGCTTC AGGACAAAGC CTGGCCTCTG TTGTGTCTGC TCAGAAAATT 
ACAAAGCCTG CTGCTAAATA TGGCGTGCCT GTGGCATATA AGAAACATGG AGATAAAAAA 
TTGCAGGAAA AGAAGCCACT CCAAAAACTT AAGCAGGCCC ATCAAACTCC ATTGAAGAAA 
GTGAATCCTG GAGAAGAAAG GGGGAAAATG TTTGAGGAAG GAGCAAGAAA GAGAAGGTTG 
GGATTTATCG AAAAAGAAAA GAAACAAAAA GATCAGATTA GTTTAATGAA GGCTGAACAG 
ATGAAAAGGC AAGAAAAGGA AAGGTTGGAG AGAATAAATA GGGCCAGGGA ACAAGGATGG 
AGAAATGTGC TAAGTGCTGG TGGAAGTGGT GAAGTAAAGG CTACCTTTTT TGGCGGTGGA 
GGGGCTGTGG CTCCATCATT TCCTTCTCGA GGACAATATG AACATTATCA TGCTATTTTT 
GATCAAATGC AGCGACAAAG AGCAGAAGAT AATGAAGCTA AATGGAAAGG AGAAATACGT 
GGCCGAAGGC TTCCAGAGAG GCAAAAGGGG CAGCTAGCTA TGGAAAGAGC TAAACAAGTG 
GAAGAATTCT TACAGCGAAA ACAGGAAGCG AGGCAGAACA AAGCTCGAGC CGAAGGGCAC 
ATGGGTAAAT ACCCAGGAGT GGGTTTGCTT GATCGTATGG GATTCCTGCA AAACCTGGCA 
GCTATGTATG GAGGCAGGTC CAGCTCTTCA AGAGGAGGGA AGCCAAGAAA CAAAGAGGAA 
GAGGTTTATC TTGCAAGGCT GAGACAAATA AGATTGCAGA ATTTCAATGA GCGCCAACAG 
ATTAGAGCCA AACTTCGTGG TGAAAAGAAA GAAGTGGATA ATTCTGAAGG ACAAGAGGGA 
AGTGAGGAGA CTGACATGAG GCGTAAAAAA GTTGAAGCAC TTAAGGCCCA AGCAAGTGCA 
CGTGCTTCTG TATTAAAAGA ACAATTGGAA CGAAAGAGAA AGGAAGCGTA TGAGAGAGAA 
AAGAAAGTAT GGGAAGAGCA TTTGGTGGCT AAAGGAGTAA AGAATTCTGA TATGTCTCCT 
TCAGGACAGC ATGATTCAGG TGGCTCTCCA TCAAAGCAAC AGATGAGATC TGTTATTTCT 
GTCACTTCAG CTTTGAAAGA AGTGGGTATG GACAGTTTAA CTGGTACCCA GGAACCCGCA 
GAAGAAATGC AAAAGACTAA CAATGCTATT TCGAGTAAGC GAGAAATACT ACGTAGATTA 
AATCAAAATC TTAAAGCTCA AGAAGATGAA AAAGGCAAGC AGTGTCACTC TGATACCTGT 
GAGATAATCG TTCACGAAGA TGACAAAGGA CATGAAAAAG AAAAGTCAGT TTCATCTGAT 
CGCAAGAAAT GGGAGGCAGG AGGGCAGCTC GTGATTCCTC TGGATGAGTC TGCATGGGAT 
GCATCCTTCT CTGCAACCGA CAACCATACA GTGGGAGATG TTATTAAATT AGATTCTGGA 
GGATCTCCAA GAAGAGTCTG GGGGAAAAGC CCTACAGATT CTGTTCTAAA AATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTGGGAAACA CAACCATTAG AAGTGATATT 
TCTTCTGAAG GGGAGAAGTA CAAACCCTTA ATCACTGGAG AAGAAAAAGT AAAATGTATT 
TCACTTGAAA TGAACCTATC AGCTACTCTC CATTCTTCTG CTGAGACAAG CAGTCCAAAG 
CTGAGTGAGG GATCTCCACA GATGTCATTG AAACCAGAAG GAAATTTGGA AGAACGTGAT 
GACTTGGAAA CAGAAGTTTT ACAAGAGTCA AGTGGAGCAA ACAAAGATGG TAGCTTGCCT 
TGCACTGTTA TGGATGTGTG GATTAGTGAG ATAAAAGAAA CAAAGGAAAC TAAATTGGAA 
GATAGGATTA CTAGTCAGAA AAATGAAGAT TCTGAAGATG GATTTCTGAG GAATCTGGAA 
CAACTTAGTG AAGTTCACAT AGAACCTGGA ATAGATGATT CTCAGCACTC TTATAAATGT 
GATACAGACA AATCTATACA ACCAGAACCA TTTTTCCATA AAGTGGTTCA TTCCGAACAC 
TTGAATGTAG TCTCTCAAAT TCAGTCAATT CTGTGTTCAC CAGAAGAAGC CTTGCCACTT 
CGATCTCGCT CTGATTCACC ACCAAAAAAT AAAAACAAGA ATTCTTTGCT GATTGGACTT 
TCAACTGGTC TTTTTGATGC AAATAACCCA AAGATGCTAA GGACATGTTC ACTTCCAGAT 
CTCTCAAAGC TGTTCAGAAC ACTGATAGAT GTTCCTACTA TAGGAGATGT ACATCAAGAC 
AATCTTGAAA TAGATGAAAT TGAAGATGAG CACATTAAAG AAGGACCTTC TGATTCTGAA 
GACATTGTTT TTGAAGAAAC TGACACAGAT TTACAGGAGC TTCAGGCCTC TATGGAGCAG 
TTACTTAGGG AACAGCCTGG GGAAGAATAC AGTGAGGAGG AAGAATCAGT CTTAAAGAAC 
AGTGACATAG AGCAGACTGC AAATGGGACA GATCTGGCAG ATGAGGATGA CAATCCCAGT 
AGTGAAAGTG CTCTAAATGA AGAATGGCAC TCAGATAACA GCGATGGTGA GATAACTAGT 
GAATGTGAAT ATGATAGTGT CTTTAACCAT TTAGAGGAAC TGAGACTTCA CCTGGAGCAG 
GAAATAGGCT TTGAAAAGTT CTTTGAGGTT TATGAGAAAA TAAAGTCTAT TCATGAAGAT 
GAAGATGAAA ATATTGAGAT TTGTTCAACA ATAGCTCAGA ATATTTTGGG AAATGAACAT 
CAACATCTTT ATGCCAAGAT CCTTCATTTA GTTATGGCAG ATGGAGCCTA TCAAGAAGAT 
AATGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011360279.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358581.1 Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPt46894
Clone ID Related Accession (Same CDS sequence)	XM_011360279.1 , XM_011360279.2
Accession Version	XM_011360279.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888834.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011360279.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGAACATATT TTTAACCAAA GATGGGACAA TACAACTTGG AGATTTTGGA 
ATTGCTAGAG TTCTTAATAG TACCGTAGAA CTTGCTCGAA CCTGCATAGG GACCCCATAC 
TACTTGTCAC CTGAAATCTG TGAAAACAAA CCTTACAACA ATAAAAGTGA CATTTGGGCT 
CTTGGATGTG TCCTTTATGA GATGTGCACA CTTAAACATG CATTTGAAGC TGGCAATATG 
AAAAACCTGG TACTGAAGAT AATATCTGGG TCTTTTCCAC CTGTGTCTTT GCATTATTCT 
TATGATCTCC GCAGTTTGCT CTCTCAGTTA TTTAAAAGAA ATCCTAGGGA TAGACCATCC 
GTCAACTCCA TATTGGAGAA AGGTTTTATA GCCAAACGCA TTGAAAAGTT TCTCTCACCT 
CAGCTTATTG CAGAAGAATT TTGTCTAAAA ACATTTTCCA AGTTTGGACC TCAACTTATA 
CCAGCTAAAA GACCAGCTTC AGGACAAAGC CTGGCCTCTG TTGTGTCTGC TCAGAAAATT 
ACAAAGCCTG CTGCTAAATA TGGCGTGCCT GTGGCATATA AGAAACATGG AGATAAAAAA 
TTGCAGGAAA AGAAGCCACT CCAAAAACTT AAGCAGGCCC ATCAAACTCC ATTGAAGAAA 
GTGAATCCTG GAGAAGAAAG GGGGAAAATG TTTGAGGAAG GAGCAAGAAA GAGAAGGTTG 
GGATTTATCG AAAAAGAAAA GAAACAAAAA GATCAGATTA GTTTAATGAA GGCTGAACAG 
ATGAAAAGGC AAGAAAAGGA AAGGTTGGAG AGAATAAATA GGGCCAGGGA ACAAGGATGG 
AGAAATGTGC TAAGTGCTGG TGGAAGTGGT GAAGTAAAGG CTACCTTTTT TGGCGGTGGA 
GGGGCTGTGG CTCCATCATT TCCTTCTCGA GGACAATATG AACATTATCA TGCTATTTTT 
GATCAAATGC AGCGACAAAG AGCAGAAGAT AATGAAGCTA AATGGAAAGG AGAAATACGT 
GGCCGAAGGC TTCCAGAGAG GCAAAAGGGG CAGCTAGCTA TGGAAAGAGC TAAACAAGTG 
GAAGAATTCT TACAGCGAAA ACAGGAAGCG AGGCAGAACA AAGCTCGAGC CGAAGGGCAC 
ATGGGTAAAT ACCCAGGAGT GGGTTTGCTT GATCGTATGG GATTCCTGCA AAACCTGGCA 
GCTATGTATG GAGGCAGGTC CAGCTCTTCA AGAGGAGGGA AGCCAAGAAA CAAAGAGGAA 
GAGGTTTATC TTGCAAGGCT GAGACAAATA AGATTGCAGA ATTTCAATGA GCGCCAACAG 
ATTAGAGCCA AACTTCGTGG TGAAAAGAAA GAAGTGGATA ATTCTGAAGG ACAAGAGGGA 
AGTGAGGAGA CTGACATGAG GCGTAAAAAA GTTGAAGCAC TTAAGGCCCA AGCAAGTGCA 
CGTGCTTCTG TATTAAAAGA ACAATTGGAA CGAAAGAGAA AGGAAGCGTA TGAGAGAGAA 
AAGAAAGTAT GGGAAGAGCA TTTGGTGGCT AAAGGAGTAA AGAATTCTGA TATGTCTCCT 
TCAGGACAGC ATGATTCAGG TGGCTCTCCA TCAAAGCAAC AGATGAGATC TGTTATTTCT 
GTCACTTCAG CTTTGAAAGA AGTGGGTATG GACAGTTTAA CTGGTACCCA GGAACCCGCA 
GAAGAAATGC AAAAGACTAA CAATGCTATT TCGAGTAAGC GAGAAATACT ACGTAGATTA 
AATCAAAATC TTAAAGCTCA AGAAGATGAA AAAGGCAAGC AGTGTCACTC TGATACCTGT 
GAGATAATCG TTCACGAAGA TGACAAAGGA CATGAAAAAG AAAAGTCAGT TTCATCTGAT 
CGCAAGAAAT GGGAGGCAGG AGGGCAGCTC GTGATTCCTC TGGATGAGTC TGCATGGGAT 
GCATCCTTCT CTGCAACCGA CAACCATACA GTGGGAGATG TTATTAAATT AGATTCTGGA 
GGATCTCCAA GAAGAGTCTG GGGGAAAAGC CCTACAGATT CTGTTCTAAA AATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTGGGAAACA CAACCATTAG AAGTGATATT 
TCTTCTGAAG GGGAGAAGTA CAAACCCTTA ATCACTGGAG AAGAAAAAGT AAAATGTATT 
TCACTTGAAA TGAACCTATC AGCTACTCTC CATTCTTCTG CTGAGACAAG CAGTCCAAAG 
CTGAGTGAGG GATCTCCACA GATGTCATTG AAACCAGAAG GAAATTTGGA AGAACGTGAT 
GACTTGGAAA CAGAAGTTTT ACAAGAGTCA AGTGGAGCAA ACAAAGATGG TAGCTTGCCT 
TGCACTGTTA TGGATGTGTG GATTAGTGAG ATAAAAGAAA CAAAGGAAAC TAAATTGGAA 
GATAGGATTA CTAGTCAGAA AAATGAAGAT TCTGAAGATG GATTTCTGAG GAATCTGGAA 
CAACTTAGTG AAGTTCACAT AGAACCTGGA ATAGATGATT CTCAGCACTC TTATAAATGT 
GATACAGACA AATCTATACA ACCAGAACCA TTTTTCCATA AAGTGGTTCA TTCCGAACAC 
TTGAATGTAG TCTCTCAAAT TCAGTCAATT CTGTGTTCAC CAGAAGAAGC CTTGCCACTT 
CGATCTCGCT CTGATTCACC ACCAAAAAAT AAAAACAAGA ATTCTTTGCT GATTGGACTT 
TCAACTGGTC TTTTTGATGC AAATAACCCA AAGATGCTAA GGACATGTTC ACTTCCAGAT 
CTCTCAAAGC TGTTCAGAAC ACTGATAGAT GTTCCTACTA TAGGAGATGT ACATCAAGAC 
AATCTTGAAA TAGATGAAAT TGAAGATGAG CACATTAAAG AAGGACCTTC TGATTCTGAA 
GACATTGTTT TTGAAGAAAC TGACACAGAT TTACAGGAGC TTCAGGCCTC TATGGAGCAG 
TTACTTAGGG AACAGCCTGG GGAAGAATAC AGTGAGGAGG AAGAATCAGT CTTAAAGAAC 
AGTGACATAG AGCAGACTGC AAATGGGACA GATCTGGCAG ATGAGGATGA CAATCCCAGT 
AGTGAAAGTG CTCTAAATGA AGAATGGCAC TCAGATAACA GCGATGGTGA GATAACTAGT 
GAATGTGAAT ATGATAGTGT CTTTAACCAT TTAGAGGAAC TGAGACTTCA CCTGGAGCAG 
GAAATAGGCT TTGAAAAGTT CTTTGAGGTT TATGAGAAAA TAAAGTCTAT TCATGAAGAT 
GAAGATGAAA ATATTGAGAT TTGTTCAACA ATAGCTCAGA ATATTTTGGG AAATGAACAT 
CAACATCTTT ATGCCAAGAT CCTTCATTTA GTTATGGCAG ATGGAGCCTA TCAAGAAGAT 
AATGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358581.1
CDS	608..4279
Translation	MDKYVRLQKIGEGSFGKAILVKSTEDGKQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGPQLIPAKRPASGQSLASVVSAQKITKPAAKYGVPVAYKKHGDKKLQEKKPLQKLKQAHQTPLKKVNPGEERGKMFEEGARKRRLGFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKATFFGGGGAVAPSFPSRGQYEHYHAIFDQMQRQRAEDNEAKWKGEIRGRRLPERQKGQLAMERAKQVEEFLQRKQEARQNKARAEGHMGKYPGVGLLDRMGFLQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKEVDNSEGQEGSEETDMRRKKVEALKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPSGQHDSGGSPSKQQMRSVISVTSALKEVGMDSLTGTQEPAEEMQKTNNAISSKREILRRLNQNLKAQEDEKGKQCHSDTCEIIVHEDDKGHEKEKSVSSDRKKWEAGGQLVIPLDESAWDASFSATDNHTVGDVIKLDSGGSPRRVWGKSPTDSVLKILGEAELQLQTELLGNTTIRSDISSEGEKYKPLITGEEKVKCISLEMNLSATLHSSAETSSPKLSEGSPQMSLKPEGNLEERDDLETEVLQESSGANKDGSLPCTVMDVWISEIKETKETKLEDRITSQKNEDSEDGFLRNLEQLSEVHIEPGIDDSQHSYKCDTDKSIQPEPFFHKVVHSEHLNVVSQIQSILCSPEEALPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLIDVPTIGDVHQDNLEIDEIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKSIHEDEDENIEICSTIAQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011360279.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360279.2

ORF Insert Sequence:

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGAACATATT TTTAACCAAA GATGGGACAA TACAACTTGG AGATTTTGGA 
ATTGCTAGAG TTCTTAATAG TACCGTAGAA CTTGCTCGAA CCTGCATAGG GACCCCATAC 
TACTTGTCAC CTGAAATCTG TGAAAACAAA CCTTACAACA ATAAAAGTGA CATTTGGGCT 
CTTGGATGTG TCCTTTATGA GATGTGCACA CTTAAACATG CATTTGAAGC TGGCAATATG 
AAAAACCTGG TACTGAAGAT AATATCTGGG TCTTTTCCAC CTGTGTCTTT GCATTATTCT 
TATGATCTCC GCAGTTTGCT CTCTCAGTTA TTTAAAAGAA ATCCTAGGGA TAGACCATCC 
GTCAACTCCA TATTGGAGAA AGGTTTTATA GCCAAACGCA TTGAAAAGTT TCTCTCACCT 
CAGCTTATTG CAGAAGAATT TTGTCTAAAA ACATTTTCCA AGTTTGGACC TCAACTTATA 
CCAGCTAAAA GACCAGCTTC AGGACAAAGC CTGGCCTCTG TTGTGTCTGC TCAGAAAATT 
ACAAAGCCTG CTGCTAAATA TGGCGTGCCT GTGGCATATA AGAAACATGG AGATAAAAAA 
TTGCAGGAAA AGAAGCCACT CCAAAAACTT AAGCAGGCCC ATCAAACTCC ATTGAAGAAA 
GTGAATCCTG GAGAAGAAAG GGGGAAAATG TTTGAGGAAG GAGCAAGAAA GAGAAGGTTG 
GGATTTATCG AAAAAGAAAA GAAACAAAAA GATCAGATTA GTTTAATGAA GGCTGAACAG 
ATGAAAAGGC AAGAAAAGGA AAGGTTGGAG AGAATAAATA GGGCCAGGGA ACAAGGATGG 
AGAAATGTGC TAAGTGCTGG TGGAAGTGGT GAAGTAAAGG CTACCTTTTT TGGCGGTGGA 
GGGGCTGTGG CTCCATCATT TCCTTCTCGA GGACAATATG AACATTATCA TGCTATTTTT 
GATCAAATGC AGCGACAAAG AGCAGAAGAT AATGAAGCTA AATGGAAAGG AGAAATACGT 
GGCCGAAGGC TTCCAGAGAG GCAAAAGGGG CAGCTAGCTA TGGAAAGAGC TAAACAAGTG 
GAAGAATTCT TACAGCGAAA ACAGGAAGCG AGGCAGAACA AAGCTCGAGC CGAAGGGCAC 
ATGGGTAAAT ACCCAGGAGT GGGTTTGCTT GATCGTATGG GATTCCTGCA AAACCTGGCA 
GCTATGTATG GAGGCAGGTC CAGCTCTTCA AGAGGAGGGA AGCCAAGAAA CAAAGAGGAA 
GAGGTTTATC TTGCAAGGCT GAGACAAATA AGATTGCAGA ATTTCAATGA GCGCCAACAG 
ATTAGAGCCA AACTTCGTGG TGAAAAGAAA GAAGTGGATA ATTCTGAAGG ACAAGAGGGA 
AGTGAGGAGA CTGACATGAG GCGTAAAAAA GTTGAAGCAC TTAAGGCCCA AGCAAGTGCA 
CGTGCTTCTG TATTAAAAGA ACAATTGGAA CGAAAGAGAA AGGAAGCGTA TGAGAGAGAA 
AAGAAAGTAT GGGAAGAGCA TTTGGTGGCT AAAGGAGTAA AGAATTCTGA TATGTCTCCT 
TCAGGACAGC ATGATTCAGG TGGCTCTCCA TCAAAGCAAC AGATGAGATC TGTTATTTCT 
GTCACTTCAG CTTTGAAAGA AGTGGGTATG GACAGTTTAA CTGGTACCCA GGAACCCGCA 
GAAGAAATGC AAAAGACTAA CAATGCTATT TCGAGTAAGC GAGAAATACT ACGTAGATTA 
AATCAAAATC TTAAAGCTCA AGAAGATGAA AAAGGCAAGC AGTGTCACTC TGATACCTGT 
GAGATAATCG TTCACGAAGA TGACAAAGGA CATGAAAAAG AAAAGTCAGT TTCATCTGAT 
CGCAAGAAAT GGGAGGCAGG AGGGCAGCTC GTGATTCCTC TGGATGAGTC TGCATGGGAT 
GCATCCTTCT CTGCAACCGA CAACCATACA GTGGGAGATG TTATTAAATT AGATTCTGGA 
GGATCTCCAA GAAGAGTCTG GGGGAAAAGC CCTACAGATT CTGTTCTAAA AATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTGGGAAACA CAACCATTAG AAGTGATATT 
TCTTCTGAAG GGGAGAAGTA CAAACCCTTA ATCACTGGAG AAGAAAAAGT AAAATGTATT 
TCACTTGAAA TGAACCTATC AGCTACTCTC CATTCTTCTG CTGAGACAAG CAGTCCAAAG 
CTGAGTGAGG GATCTCCACA GATGTCATTG AAACCAGAAG GAAATTTGGA AGAACGTGAT 
GACTTGGAAA CAGAAGTTTT ACAAGAGTCA AGTGGAGCAA ACAAAGATGG TAGCTTGCCT 
TGCACTGTTA TGGATGTGTG GATTAGTGAG ATAAAAGAAA CAAAGGAAAC TAAATTGGAA 
GATAGGATTA CTAGTCAGAA AAATGAAGAT TCTGAAGATG GATTTCTGAG GAATCTGGAA 
CAACTTAGTG AAGTTCACAT AGAACCTGGA ATAGATGATT CTCAGCACTC TTATAAATGT 
GATACAGACA AATCTATACA ACCAGAACCA TTTTTCCATA AAGTGGTTCA TTCCGAACAC 
TTGAATGTAG TCTCTCAAAT TCAGTCAATT CTGTGTTCAC CAGAAGAAGC CTTGCCACTT 
CGATCTCGCT CTGATTCACC ACCAAAAAAT AAAAACAAGA ATTCTTTGCT GATTGGACTT 
TCAACTGGTC TTTTTGATGC AAATAACCCA AAGATGCTAA GGACATGTTC ACTTCCAGAT 
CTCTCAAAGC TGTTCAGAAC ACTGATAGAT GTTCCTACTA TAGGAGATGT ACATCAAGAC 
AATCTTGAAA TAGATGAAAT TGAAGATGAG CACATTAAAG AAGGACCTTC TGATTCTGAA 
GACATTGTTT TTGAAGAAAC TGACACAGAT TTACAGGAGC TTCAGGCCTC TATGGAGCAG 
TTACTTAGGG AACAGCCTGG GGAAGAATAC AGTGAGGAGG AAGAATCAGT CTTAAAGAAC 
AGTGACATAG AGCAGACTGC AAATGGGACA GATCTGGCAG ATGAGGATGA CAATCCCAGT 
AGTGAAAGTG CTCTAAATGA AGAATGGCAC TCAGATAACA GCGATGGTGA GATAACTAGT 
GAATGTGAAT ATGATAGTGT CTTTAACCAT TTAGAGGAAC TGAGACTTCA CCTGGAGCAG 
GAAATAGGCT TTGAAAAGTT CTTTGAGGTT TATGAGAAAA TAAAGTCTAT TCATGAAGAT 
GAAGATGAAA ATATTGAGAT TTGTTCAACA ATAGCTCAGA ATATTTTGGG AAATGAACAT 
CAACATCTTT ATGCCAAGAT CCTTCATTTA GTTATGGCAG ATGGAGCCTA TCAAGAAGAT 
AATGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011360281.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358583.1 Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

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CloneID	OPt46895
Clone ID Related Accession (Same CDS sequence)	XM_011360281.2 , XM_011360281.1
Accession Version	XM_011360281.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888834.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011360281.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTGACAT TTGGGCTCTT 
GGATGTGTCC TTTATGAGAT GTGCACACTT AAACATGCAT TTGAAGCTGG CAATATGAAA 
AACCTGGTAC TGAAGATAAT ATCTGGGTCT TTTCCACCTG TGTCTTTGCA TTATTCTTAT 
GATCTCCGCA GTTTGCTCTC TCAGTTATTT AAAAGAAATC CTAGGGATAG ACCATCCGTC 
AACTCCATAT TGGAGAAAGG TTTTATAGCC AAACGCATTG AAAAGTTTCT CTCACCTCAG 
CTTATTGCAG AAGAATTTTG TCTAAAAACA TTTTCCAAGT TTGGACCTCA ACTTATACCA 
GCTAAAAGAC CAGCTTCAGG ACAAAGCCTG GCCTCTGTTG TGTCTGCTCA GAAAATTACA 
AAGCCTGCTG CTAAATATGG CGTGCCTGTG GCATATAAGA AACATGGAGA TAAAAAATTG 
CAGGAAAAGA AGCCACTCCA AAAACTTAAG CAGGCCCATC AAACTCCATT GAAGAAAGTG 
AATCCTGGAG AAGAAAGGGG GAAAATGTTT GAGGAAGGAG CAAGAAAGAG AAGGTTGGGA 
TTTATCGAAA AAGAAAAGAA ACAAAAAGAT CAGATTAGTT TAATGAAGGC TGAACAGATG 
AAAAGGCAAG AAAAGGAAAG GTTGGAGAGA ATAAATAGGG CCAGGGAACA AGGATGGAGA 
AATGTGCTAA GTGCTGGTGG AAGTGGTGAA GTAAAGGCTA CCTTTTTTGG CGGTGGAGGG 
GCTGTGGCTC CATCATTTCC TTCTCGAGGA CAATATGAAC ATTATCATGC TATTTTTGAT 
CAAATGCAGC GACAAAGAGC AGAAGATAAT GAAGCTAAAT GGAAAGGAGA AATACGTGGC 
CGAAGGCTTC CAGAGAGGCA AAAGGGGCAG CTAGCTATGG AAAGAGCTAA ACAAGTGGAA 
GAATTCTTAC AGCGAAAACA GGAAGCGAGG CAGAACAAAG CTCGAGCCGA AGGGCACATG 
GGTAAATACC CAGGAGTGGG TTTGCTTGAT CGTATGGGAT TCCTGCAAAA CCTGGCAGCT 
ATGTATGGAG GCAGGTCCAG CTCTTCAAGA GGAGGGAAGC CAAGAAACAA AGAGGAAGAG 
GTTTATCTTG CAAGGCTGAG ACAAATAAGA TTGCAGAATT TCAATGAGCG CCAACAGATT 
AGAGCCAAAC TTCGTGGTGA AAAGAAAGAA GTGGATAATT CTGAAGGACA AGAGGGAAGT 
GAGGAGACTG ACATGAGGCG TAAAAAAGTT GAAGCACTTA AGGCCCAAGC AAGTGCACGT 
GCTTCTGTAT TAAAAGAACA ATTGGAACGA AAGAGAAAGG AAGCGTATGA GAGAGAAAAG 
AAAGTATGGG AAGAGCATTT GGTGGCTAAA GGAGTAAAGA ATTCTGATAT GTCTCCTTCA 
GGACAGCATG ATTCAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCTGT TATTTCTGTC 
ACTTCAGCTT TGAAAGAAGT GGGTATGGAC AGTTTAACTG GTACCCAGGA ACCCGCAGAA 
GAAATGCAAA AGACTAACAA TGCTATTTCG AGTAAGCGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAAAAA GGCAAGCAGT GTCACTCTGA TACCTGTGAG 
ATAATCGTTC ACGAAGATGA CAAAGGACAT GAAAAAGAAA AGTCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG GCAGCTCGTG ATTCCTCTGG ATGAGTCTGC ATGGGATGCA 
TCCTTCTCTG CAACCGACAA CCATACAGTG GGAGATGTTA TTAAATTAGA TTCTGGAGGA 
TCTCCAAGAA GAGTCTGGGG GAAAAGCCCT ACAGATTCTG TTCTAAAAAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTATTG GGAAACACAA CCATTAGAAG TGATATTTCT 
TCTGAAGGGG AGAAGTACAA ACCCTTAATC ACTGGAGAAG AAAAAGTAAA ATGTATTTCA 
CTTGAAATGA ACCTATCAGC TACTCTCCAT TCTTCTGCTG AGACAAGCAG TCCAAAGCTG 
AGTGAGGGAT CTCCACAGAT GTCATTGAAA CCAGAAGGAA ATTTGGAAGA ACGTGATGAC 
TTGGAAACAG AAGTTTTACA AGAGTCAAGT GGAGCAAACA AAGATGGTAG CTTGCCTTGC 
ACTGTTATGG ATGTGTGGAT TAGTGAGATA AAAGAAACAA AGGAAACTAA ATTGGAAGAT 
AGGATTACTA GTCAGAAAAA TGAAGATTCT GAAGATGGAT TTCTGAGGAA TCTGGAACAA 
CTTAGTGAAG TTCACATAGA ACCTGGAATA GATGATTCTC AGCACTCTTA TAAATGTGAT 
ACAGACAAAT CTATACAACC AGAACCATTT TTCCATAAAG TGGTTCATTC CGAACACTTG 
AATGTAGTCT CTCAAATTCA GTCAATTCTG TGTTCACCAG AAGAAGCCTT GCCACTTCGA 
TCTCGCTCTG ATTCACCACC AAAAAATAAA AACAAGAATT CTTTGCTGAT TGGACTTTCA 
ACTGGTCTTT TTGATGCAAA TAACCCAAAG ATGCTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACACT GATAGATGTT CCTACTATAG GAGATGTACA TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAGCAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTTTTTG AAGAAACTGA CACAGATTTA CAGGAGCTTC AGGCCTCTAT GGAGCAGTTA 
CTTAGGGAAC AGCCTGGGGA AGAATACAGT GAGGAGGAAG AATCAGTCTT AAAGAACAGT 
GACATAGAGC AGACTGCAAA TGGGACAGAT CTGGCAGATG AGGATGACAA TCCCAGTAGT 
GAAAGTGCTC TAAATGAAGA ATGGCACTCA GATAACAGCG ATGGTGAGAT AACTAGTGAA 
TGTGAATATG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCACCT GGAGCAGGAA 
ATAGGCTTTG AAAAGTTCTT TGAGGTTTAT GAGAAAATAA AGTCTATTCA TGAAGATGAA 
GATGAAAATA TTGAGATTTG TTCAACAATA GCTCAGAATA TTTTGGGAAA TGAACATCAA 
CATCTTTATG CCAAGATCCT TCATTTAGTT ATGGCAGATG GAGCCTATCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358583.1
CDS	608..4276
Translation	MDKYVRLQKIGEGSFGKAILVKSTEDGKQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGPQLIPAKRPASGQSLASVVSAQKITKPAAKYGVPVAYKKHGDKKLQEKKPLQKLKQAHQTPLKKVNPGEERGKMFEEGARKRRLGFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKATFFGGGGAVAPSFPSRGQYEHYHAIFDQMQRQRAEDNEAKWKGEIRGRRLPERQKGQLAMERAKQVEEFLQRKQEARQNKARAEGHMGKYPGVGLLDRMGFLQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKEVDNSEGQEGSEETDMRRKKVEALKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPSGQHDSGGSPSKQQMRSVISVTSALKEVGMDSLTGTQEPAEEMQKTNNAISSKREILRRLNQNLKAQEDEKGKQCHSDTCEIIVHEDDKGHEKEKSVSSDRKKWEAGGQLVIPLDESAWDASFSATDNHTVGDVIKLDSGGSPRRVWGKSPTDSVLKILGEAELQLQTELLGNTTIRSDISSEGEKYKPLITGEEKVKCISLEMNLSATLHSSAETSSPKLSEGSPQMSLKPEGNLEERDDLETEVLQESSGANKDGSLPCTVMDVWISEIKETKETKLEDRITSQKNEDSEDGFLRNLEQLSEVHIEPGIDDSQHSYKCDTDKSIQPEPFFHKVVHSEHLNVVSQIQSILCSPEEALPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLIDVPTIGDVHQDNLEIDEIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKSIHEDEDENIEICSTIAQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011360281.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360281.2

ORF Insert Sequence:

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTGACAT TTGGGCTCTT 
GGATGTGTCC TTTATGAGAT GTGCACACTT AAACATGCAT TTGAAGCTGG CAATATGAAA 
AACCTGGTAC TGAAGATAAT ATCTGGGTCT TTTCCACCTG TGTCTTTGCA TTATTCTTAT 
GATCTCCGCA GTTTGCTCTC TCAGTTATTT AAAAGAAATC CTAGGGATAG ACCATCCGTC 
AACTCCATAT TGGAGAAAGG TTTTATAGCC AAACGCATTG AAAAGTTTCT CTCACCTCAG 
CTTATTGCAG AAGAATTTTG TCTAAAAACA TTTTCCAAGT TTGGACCTCA ACTTATACCA 
GCTAAAAGAC CAGCTTCAGG ACAAAGCCTG GCCTCTGTTG TGTCTGCTCA GAAAATTACA 
AAGCCTGCTG CTAAATATGG CGTGCCTGTG GCATATAAGA AACATGGAGA TAAAAAATTG 
CAGGAAAAGA AGCCACTCCA AAAACTTAAG CAGGCCCATC AAACTCCATT GAAGAAAGTG 
AATCCTGGAG AAGAAAGGGG GAAAATGTTT GAGGAAGGAG CAAGAAAGAG AAGGTTGGGA 
TTTATCGAAA AAGAAAAGAA ACAAAAAGAT CAGATTAGTT TAATGAAGGC TGAACAGATG 
AAAAGGCAAG AAAAGGAAAG GTTGGAGAGA ATAAATAGGG CCAGGGAACA AGGATGGAGA 
AATGTGCTAA GTGCTGGTGG AAGTGGTGAA GTAAAGGCTA CCTTTTTTGG CGGTGGAGGG 
GCTGTGGCTC CATCATTTCC TTCTCGAGGA CAATATGAAC ATTATCATGC TATTTTTGAT 
CAAATGCAGC GACAAAGAGC AGAAGATAAT GAAGCTAAAT GGAAAGGAGA AATACGTGGC 
CGAAGGCTTC CAGAGAGGCA AAAGGGGCAG CTAGCTATGG AAAGAGCTAA ACAAGTGGAA 
GAATTCTTAC AGCGAAAACA GGAAGCGAGG CAGAACAAAG CTCGAGCCGA AGGGCACATG 
GGTAAATACC CAGGAGTGGG TTTGCTTGAT CGTATGGGAT TCCTGCAAAA CCTGGCAGCT 
ATGTATGGAG GCAGGTCCAG CTCTTCAAGA GGAGGGAAGC CAAGAAACAA AGAGGAAGAG 
GTTTATCTTG CAAGGCTGAG ACAAATAAGA TTGCAGAATT TCAATGAGCG CCAACAGATT 
AGAGCCAAAC TTCGTGGTGA AAAGAAAGAA GTGGATAATT CTGAAGGACA AGAGGGAAGT 
GAGGAGACTG ACATGAGGCG TAAAAAAGTT GAAGCACTTA AGGCCCAAGC AAGTGCACGT 
GCTTCTGTAT TAAAAGAACA ATTGGAACGA AAGAGAAAGG AAGCGTATGA GAGAGAAAAG 
AAAGTATGGG AAGAGCATTT GGTGGCTAAA GGAGTAAAGA ATTCTGATAT GTCTCCTTCA 
GGACAGCATG ATTCAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCTGT TATTTCTGTC 
ACTTCAGCTT TGAAAGAAGT GGGTATGGAC AGTTTAACTG GTACCCAGGA ACCCGCAGAA 
GAAATGCAAA AGACTAACAA TGCTATTTCG AGTAAGCGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAAAAA GGCAAGCAGT GTCACTCTGA TACCTGTGAG 
ATAATCGTTC ACGAAGATGA CAAAGGACAT GAAAAAGAAA AGTCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG GCAGCTCGTG ATTCCTCTGG ATGAGTCTGC ATGGGATGCA 
TCCTTCTCTG CAACCGACAA CCATACAGTG GGAGATGTTA TTAAATTAGA TTCTGGAGGA 
TCTCCAAGAA GAGTCTGGGG GAAAAGCCCT ACAGATTCTG TTCTAAAAAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTATTG GGAAACACAA CCATTAGAAG TGATATTTCT 
TCTGAAGGGG AGAAGTACAA ACCCTTAATC ACTGGAGAAG AAAAAGTAAA ATGTATTTCA 
CTTGAAATGA ACCTATCAGC TACTCTCCAT TCTTCTGCTG AGACAAGCAG TCCAAAGCTG 
AGTGAGGGAT CTCCACAGAT GTCATTGAAA CCAGAAGGAA ATTTGGAAGA ACGTGATGAC 
TTGGAAACAG AAGTTTTACA AGAGTCAAGT GGAGCAAACA AAGATGGTAG CTTGCCTTGC 
ACTGTTATGG ATGTGTGGAT TAGTGAGATA AAAGAAACAA AGGAAACTAA ATTGGAAGAT 
AGGATTACTA GTCAGAAAAA TGAAGATTCT GAAGATGGAT TTCTGAGGAA TCTGGAACAA 
CTTAGTGAAG TTCACATAGA ACCTGGAATA GATGATTCTC AGCACTCTTA TAAATGTGAT 
ACAGACAAAT CTATACAACC AGAACCATTT TTCCATAAAG TGGTTCATTC CGAACACTTG 
AATGTAGTCT CTCAAATTCA GTCAATTCTG TGTTCACCAG AAGAAGCCTT GCCACTTCGA 
TCTCGCTCTG ATTCACCACC AAAAAATAAA AACAAGAATT CTTTGCTGAT TGGACTTTCA 
ACTGGTCTTT TTGATGCAAA TAACCCAAAG ATGCTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACACT GATAGATGTT CCTACTATAG GAGATGTACA TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAGCAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTTTTTG AAGAAACTGA CACAGATTTA CAGGAGCTTC AGGCCTCTAT GGAGCAGTTA 
CTTAGGGAAC AGCCTGGGGA AGAATACAGT GAGGAGGAAG AATCAGTCTT AAAGAACAGT 
GACATAGAGC AGACTGCAAA TGGGACAGAT CTGGCAGATG AGGATGACAA TCCCAGTAGT 
GAAAGTGCTC TAAATGAAGA ATGGCACTCA GATAACAGCG ATGGTGAGAT AACTAGTGAA 
TGTGAATATG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCACCT GGAGCAGGAA 
ATAGGCTTTG AAAAGTTCTT TGAGGTTTAT GAGAAAATAA AGTCTATTCA TGAAGATGAA 
GATGAAAATA TTGAGATTTG TTCAACAATA GCTCAGAATA TTTTGGGAAA TGAACATCAA 
CATCTTTATG CCAAGATCCT TCATTTAGTT ATGGCAGATG GAGCCTATCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011360281.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358583.1 Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPt46895
Clone ID Related Accession (Same CDS sequence)	XM_011360281.2 , XM_011360281.1
Accession Version	XM_011360281.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888834.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTGACAT TTGGGCTCTT 
GGATGTGTCC TTTATGAGAT GTGCACACTT AAACATGCAT TTGAAGCTGG CAATATGAAA 
AACCTGGTAC TGAAGATAAT ATCTGGGTCT TTTCCACCTG TGTCTTTGCA TTATTCTTAT 
GATCTCCGCA GTTTGCTCTC TCAGTTATTT AAAAGAAATC CTAGGGATAG ACCATCCGTC 
AACTCCATAT TGGAGAAAGG TTTTATAGCC AAACGCATTG AAAAGTTTCT CTCACCTCAG 
CTTATTGCAG AAGAATTTTG TCTAAAAACA TTTTCCAAGT TTGGACCTCA ACTTATACCA 
GCTAAAAGAC CAGCTTCAGG ACAAAGCCTG GCCTCTGTTG TGTCTGCTCA GAAAATTACA 
AAGCCTGCTG CTAAATATGG CGTGCCTGTG GCATATAAGA AACATGGAGA TAAAAAATTG 
CAGGAAAAGA AGCCACTCCA AAAACTTAAG CAGGCCCATC AAACTCCATT GAAGAAAGTG 
AATCCTGGAG AAGAAAGGGG GAAAATGTTT GAGGAAGGAG CAAGAAAGAG AAGGTTGGGA 
TTTATCGAAA AAGAAAAGAA ACAAAAAGAT CAGATTAGTT TAATGAAGGC TGAACAGATG 
AAAAGGCAAG AAAAGGAAAG GTTGGAGAGA ATAAATAGGG CCAGGGAACA AGGATGGAGA 
AATGTGCTAA GTGCTGGTGG AAGTGGTGAA GTAAAGGCTA CCTTTTTTGG CGGTGGAGGG 
GCTGTGGCTC CATCATTTCC TTCTCGAGGA CAATATGAAC ATTATCATGC TATTTTTGAT 
CAAATGCAGC GACAAAGAGC AGAAGATAAT GAAGCTAAAT GGAAAGGAGA AATACGTGGC 
CGAAGGCTTC CAGAGAGGCA AAAGGGGCAG CTAGCTATGG AAAGAGCTAA ACAAGTGGAA 
GAATTCTTAC AGCGAAAACA GGAAGCGAGG CAGAACAAAG CTCGAGCCGA AGGGCACATG 
GGTAAATACC CAGGAGTGGG TTTGCTTGAT CGTATGGGAT TCCTGCAAAA CCTGGCAGCT 
ATGTATGGAG GCAGGTCCAG CTCTTCAAGA GGAGGGAAGC CAAGAAACAA AGAGGAAGAG 
GTTTATCTTG CAAGGCTGAG ACAAATAAGA TTGCAGAATT TCAATGAGCG CCAACAGATT 
AGAGCCAAAC TTCGTGGTGA AAAGAAAGAA GTGGATAATT CTGAAGGACA AGAGGGAAGT 
GAGGAGACTG ACATGAGGCG TAAAAAAGTT GAAGCACTTA AGGCCCAAGC AAGTGCACGT 
GCTTCTGTAT TAAAAGAACA ATTGGAACGA AAGAGAAAGG AAGCGTATGA GAGAGAAAAG 
AAAGTATGGG AAGAGCATTT GGTGGCTAAA GGAGTAAAGA ATTCTGATAT GTCTCCTTCA 
GGACAGCATG ATTCAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCTGT TATTTCTGTC 
ACTTCAGCTT TGAAAGAAGT GGGTATGGAC AGTTTAACTG GTACCCAGGA ACCCGCAGAA 
GAAATGCAAA AGACTAACAA TGCTATTTCG AGTAAGCGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAAAAA GGCAAGCAGT GTCACTCTGA TACCTGTGAG 
ATAATCGTTC ACGAAGATGA CAAAGGACAT GAAAAAGAAA AGTCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG GCAGCTCGTG ATTCCTCTGG ATGAGTCTGC ATGGGATGCA 
TCCTTCTCTG CAACCGACAA CCATACAGTG GGAGATGTTA TTAAATTAGA TTCTGGAGGA 
TCTCCAAGAA GAGTCTGGGG GAAAAGCCCT ACAGATTCTG TTCTAAAAAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTATTG GGAAACACAA CCATTAGAAG TGATATTTCT 
TCTGAAGGGG AGAAGTACAA ACCCTTAATC ACTGGAGAAG AAAAAGTAAA ATGTATTTCA 
CTTGAAATGA ACCTATCAGC TACTCTCCAT TCTTCTGCTG AGACAAGCAG TCCAAAGCTG 
AGTGAGGGAT CTCCACAGAT GTCATTGAAA CCAGAAGGAA ATTTGGAAGA ACGTGATGAC 
TTGGAAACAG AAGTTTTACA AGAGTCAAGT GGAGCAAACA AAGATGGTAG CTTGCCTTGC 
ACTGTTATGG ATGTGTGGAT TAGTGAGATA AAAGAAACAA AGGAAACTAA ATTGGAAGAT 
AGGATTACTA GTCAGAAAAA TGAAGATTCT GAAGATGGAT TTCTGAGGAA TCTGGAACAA 
CTTAGTGAAG TTCACATAGA ACCTGGAATA GATGATTCTC AGCACTCTTA TAAATGTGAT 
ACAGACAAAT CTATACAACC AGAACCATTT TTCCATAAAG TGGTTCATTC CGAACACTTG 
AATGTAGTCT CTCAAATTCA GTCAATTCTG TGTTCACCAG AAGAAGCCTT GCCACTTCGA 
TCTCGCTCTG ATTCACCACC AAAAAATAAA AACAAGAATT CTTTGCTGAT TGGACTTTCA 
ACTGGTCTTT TTGATGCAAA TAACCCAAAG ATGCTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACACT GATAGATGTT CCTACTATAG GAGATGTACA TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAGCAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTTTTTG AAGAAACTGA CACAGATTTA CAGGAGCTTC AGGCCTCTAT GGAGCAGTTA 
CTTAGGGAAC AGCCTGGGGA AGAATACAGT GAGGAGGAAG AATCAGTCTT AAAGAACAGT 
GACATAGAGC AGACTGCAAA TGGGACAGAT CTGGCAGATG AGGATGACAA TCCCAGTAGT 
GAAAGTGCTC TAAATGAAGA ATGGCACTCA GATAACAGCG ATGGTGAGAT AACTAGTGAA 
TGTGAATATG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCACCT GGAGCAGGAA 
ATAGGCTTTG AAAAGTTCTT TGAGGTTTAT GAGAAAATAA AGTCTATTCA TGAAGATGAA 
GATGAAAATA TTGAGATTTG TTCAACAATA GCTCAGAATA TTTTGGGAAA TGAACATCAA 
CATCTTTATG CCAAGATCCT TCATTTAGTT ATGGCAGATG GAGCCTATCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358583.1
CDS	590..4258
Translation	MDKYVRLQKIGEGSFGKAILVKSTEDGKQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGPQLIPAKRPASGQSLASVVSAQKITKPAAKYGVPVAYKKHGDKKLQEKKPLQKLKQAHQTPLKKVNPGEERGKMFEEGARKRRLGFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKATFFGGGGAVAPSFPSRGQYEHYHAIFDQMQRQRAEDNEAKWKGEIRGRRLPERQKGQLAMERAKQVEEFLQRKQEARQNKARAEGHMGKYPGVGLLDRMGFLQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKEVDNSEGQEGSEETDMRRKKVEALKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPSGQHDSGGSPSKQQMRSVISVTSALKEVGMDSLTGTQEPAEEMQKTNNAISSKREILRRLNQNLKAQEDEKGKQCHSDTCEIIVHEDDKGHEKEKSVSSDRKKWEAGGQLVIPLDESAWDASFSATDNHTVGDVIKLDSGGSPRRVWGKSPTDSVLKILGEAELQLQTELLGNTTIRSDISSEGEKYKPLITGEEKVKCISLEMNLSATLHSSAETSSPKLSEGSPQMSLKPEGNLEERDDLETEVLQESSGANKDGSLPCTVMDVWISEIKETKETKLEDRITSQKNEDSEDGFLRNLEQLSEVHIEPGIDDSQHSYKCDTDKSIQPEPFFHKVVHSEHLNVVSQIQSILCSPEEALPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLIDVPTIGDVHQDNLEIDEIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKSIHEDEDENIEICSTIAQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011360281.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360281.1

ORF Insert Sequence:

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTGACAT TTGGGCTCTT 
GGATGTGTCC TTTATGAGAT GTGCACACTT AAACATGCAT TTGAAGCTGG CAATATGAAA 
AACCTGGTAC TGAAGATAAT ATCTGGGTCT TTTCCACCTG TGTCTTTGCA TTATTCTTAT 
GATCTCCGCA GTTTGCTCTC TCAGTTATTT AAAAGAAATC CTAGGGATAG ACCATCCGTC 
AACTCCATAT TGGAGAAAGG TTTTATAGCC AAACGCATTG AAAAGTTTCT CTCACCTCAG 
CTTATTGCAG AAGAATTTTG TCTAAAAACA TTTTCCAAGT TTGGACCTCA ACTTATACCA 
GCTAAAAGAC CAGCTTCAGG ACAAAGCCTG GCCTCTGTTG TGTCTGCTCA GAAAATTACA 
AAGCCTGCTG CTAAATATGG CGTGCCTGTG GCATATAAGA AACATGGAGA TAAAAAATTG 
CAGGAAAAGA AGCCACTCCA AAAACTTAAG CAGGCCCATC AAACTCCATT GAAGAAAGTG 
AATCCTGGAG AAGAAAGGGG GAAAATGTTT GAGGAAGGAG CAAGAAAGAG AAGGTTGGGA 
TTTATCGAAA AAGAAAAGAA ACAAAAAGAT CAGATTAGTT TAATGAAGGC TGAACAGATG 
AAAAGGCAAG AAAAGGAAAG GTTGGAGAGA ATAAATAGGG CCAGGGAACA AGGATGGAGA 
AATGTGCTAA GTGCTGGTGG AAGTGGTGAA GTAAAGGCTA CCTTTTTTGG CGGTGGAGGG 
GCTGTGGCTC CATCATTTCC TTCTCGAGGA CAATATGAAC ATTATCATGC TATTTTTGAT 
CAAATGCAGC GACAAAGAGC AGAAGATAAT GAAGCTAAAT GGAAAGGAGA AATACGTGGC 
CGAAGGCTTC CAGAGAGGCA AAAGGGGCAG CTAGCTATGG AAAGAGCTAA ACAAGTGGAA 
GAATTCTTAC AGCGAAAACA GGAAGCGAGG CAGAACAAAG CTCGAGCCGA AGGGCACATG 
GGTAAATACC CAGGAGTGGG TTTGCTTGAT CGTATGGGAT TCCTGCAAAA CCTGGCAGCT 
ATGTATGGAG GCAGGTCCAG CTCTTCAAGA GGAGGGAAGC CAAGAAACAA AGAGGAAGAG 
GTTTATCTTG CAAGGCTGAG ACAAATAAGA TTGCAGAATT TCAATGAGCG CCAACAGATT 
AGAGCCAAAC TTCGTGGTGA AAAGAAAGAA GTGGATAATT CTGAAGGACA AGAGGGAAGT 
GAGGAGACTG ACATGAGGCG TAAAAAAGTT GAAGCACTTA AGGCCCAAGC AAGTGCACGT 
GCTTCTGTAT TAAAAGAACA ATTGGAACGA AAGAGAAAGG AAGCGTATGA GAGAGAAAAG 
AAAGTATGGG AAGAGCATTT GGTGGCTAAA GGAGTAAAGA ATTCTGATAT GTCTCCTTCA 
GGACAGCATG ATTCAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCTGT TATTTCTGTC 
ACTTCAGCTT TGAAAGAAGT GGGTATGGAC AGTTTAACTG GTACCCAGGA ACCCGCAGAA 
GAAATGCAAA AGACTAACAA TGCTATTTCG AGTAAGCGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAAAAA GGCAAGCAGT GTCACTCTGA TACCTGTGAG 
ATAATCGTTC ACGAAGATGA CAAAGGACAT GAAAAAGAAA AGTCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG GCAGCTCGTG ATTCCTCTGG ATGAGTCTGC ATGGGATGCA 
TCCTTCTCTG CAACCGACAA CCATACAGTG GGAGATGTTA TTAAATTAGA TTCTGGAGGA 
TCTCCAAGAA GAGTCTGGGG GAAAAGCCCT ACAGATTCTG TTCTAAAAAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTATTG GGAAACACAA CCATTAGAAG TGATATTTCT 
TCTGAAGGGG AGAAGTACAA ACCCTTAATC ACTGGAGAAG AAAAAGTAAA ATGTATTTCA 
CTTGAAATGA ACCTATCAGC TACTCTCCAT TCTTCTGCTG AGACAAGCAG TCCAAAGCTG 
AGTGAGGGAT CTCCACAGAT GTCATTGAAA CCAGAAGGAA ATTTGGAAGA ACGTGATGAC 
TTGGAAACAG AAGTTTTACA AGAGTCAAGT GGAGCAAACA AAGATGGTAG CTTGCCTTGC 
ACTGTTATGG ATGTGTGGAT TAGTGAGATA AAAGAAACAA AGGAAACTAA ATTGGAAGAT 
AGGATTACTA GTCAGAAAAA TGAAGATTCT GAAGATGGAT TTCTGAGGAA TCTGGAACAA 
CTTAGTGAAG TTCACATAGA ACCTGGAATA GATGATTCTC AGCACTCTTA TAAATGTGAT 
ACAGACAAAT CTATACAACC AGAACCATTT TTCCATAAAG TGGTTCATTC CGAACACTTG 
AATGTAGTCT CTCAAATTCA GTCAATTCTG TGTTCACCAG AAGAAGCCTT GCCACTTCGA 
TCTCGCTCTG ATTCACCACC AAAAAATAAA AACAAGAATT CTTTGCTGAT TGGACTTTCA 
ACTGGTCTTT TTGATGCAAA TAACCCAAAG ATGCTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACACT GATAGATGTT CCTACTATAG GAGATGTACA TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAGCAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTTTTTG AAGAAACTGA CACAGATTTA CAGGAGCTTC AGGCCTCTAT GGAGCAGTTA 
CTTAGGGAAC AGCCTGGGGA AGAATACAGT GAGGAGGAAG AATCAGTCTT AAAGAACAGT 
GACATAGAGC AGACTGCAAA TGGGACAGAT CTGGCAGATG AGGATGACAA TCCCAGTAGT 
GAAAGTGCTC TAAATGAAGA ATGGCACTCA GATAACAGCG ATGGTGAGAT AACTAGTGAA 
TGTGAATATG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCACCT GGAGCAGGAA 
ATAGGCTTTG AAAAGTTCTT TGAGGTTTAT GAGAAAATAA AGTCTATTCA TGAAGATGAA 
GATGAAAATA TTGAGATTTG TTCAACAATA GCTCAGAATA TTTTGGGAAA TGAACATCAA 
CATCTTTATG CCAAGATCCT TCATTTAGTT ATGGCAGATG GAGCCTATCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011360282.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358584.1 Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPt46896
Clone ID Related Accession (Same CDS sequence)	XM_011360282.1 , XM_011360282.2
Accession Version	XM_011360282.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase Nek1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888834.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGTT TATCTTGCAA GGCTGAGACA AATAAGATTG 
CAGAATTTCA ATGAGCGCCA ACAGATTAGA GCCAAACTTC GTGGTGAAAA GAAAGAAGTG 
GATAATTCTG AAGGACAAGA GGGAAGTGAG GAGACTGACA TGAGGCGTAA AAAAGTTGAA 
GCACTTAAGG CCCAAGCAAG TGCACGTGCT TCTGTATTAA AAGAACAATT GGAACGAAAG 
AGAAAGGAAG CGTATGAGAG AGAAAAGAAA GTATGGGAAG AGCATTTGGT GGCTAAAGGA 
GTAAAGAATT CTGATATGTC TCCTTCAGGA CAGCATGATT CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTCACT TCAGCTTTGA AAGAAGTGGG TATGGACAGT 
TTAACTGGTA CCCAGGAACC CGCAGAAGAA ATGCAAAAGA CTAACAATGC TATTTCGAGT 
AAGCGAGAAA TACTACGTAG ATTAAATCAA AATCTTAAAG CTCAAGAAGA TGAAAAAGGC 
AAGCAGTGTC ACTCTGATAC CTGTGAGATA ATCGTTCACG AAGATGACAA AGGACATGAA 
AAAGAAAAGT CAGTTTCATC TGATCGCAAG AAATGGGAGG CAGGAGGGCA GCTCGTGATT 
CCTCTGGATG AGTCTGCATG GGATGCATCC TTCTCTGCAA CCGACAACCA TACAGTGGGA 
GATGTTATTA AATTAGATTC TGGAGGATCT CCAAGAAGAG TCTGGGGGAA AAGCCCTACA 
GATTCTGTTC TAAAAATACT TGGAGAAGCT GAACTACAAC TTCAGACAGA ACTATTGGGA 
AACACAACCA TTAGAAGTGA TATTTCTTCT GAAGGGGAGA AGTACAAACC CTTAATCACT 
GGAGAAGAAA AAGTAAAATG TATTTCACTT GAAATGAACC TATCAGCTAC TCTCCATTCT 
TCTGCTGAGA CAAGCAGTCC AAAGCTGAGT GAGGGATCTC CACAGATGTC ATTGAAACCA 
GAAGGAAATT TGGAAGAACG TGATGACTTG GAAACAGAAG TTTTACAAGA GTCAAGTGGA 
GCAAACAAAG ATGGTAGCTT GCCTTGCACT GTTATGGATG TGTGGATTAG TGAGATAAAA 
GAAACAAAGG AAACTAAATT GGAAGATAGG ATTACTAGTC AGAAAAATGA AGATTCTGAA 
GATGGATTTC TGAGGAATCT GGAACAACTT AGTGAAGTTC ACATAGAACC TGGAATAGAT 
GATTCTCAGC ACTCTTATAA ATGTGATACA GACAAATCTA TACAACCAGA ACCATTTTTC 
CATAAAGTGG TTCATTCCGA ACACTTGAAT GTAGTCTCTC AAATTCAGTC AATTCTGTGT 
TCACCAGAAG AAGCCTTGCC ACTTCGATCT CGCTCTGATT CACCACCAAA AAATAAAAAC 
AAGAATTCTT TGCTGATTGG ACTTTCAACT GGTCTTTTTG ATGCAAATAA CCCAAAGATG 
CTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACACTGAT AGATGTTCCT 
ACTATAGGAG ATGTACATCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAGCACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTTTTTGAAG AAACTGACAC AGATTTACAG 
GAGCTTCAGG CCTCTATGGA GCAGTTACTT AGGGAACAGC CTGGGGAAGA ATACAGTGAG 
GAGGAAGAAT CAGTCTTAAA GAACAGTGAC ATAGAGCAGA CTGCAAATGG GACAGATCTG 
GCAGATGAGG ATGACAATCC CAGTAGTGAA AGTGCTCTAA ATGAAGAATG GCACTCAGAT 
AACAGCGATG GTGAGATAAC TAGTGAATGT GAATATGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCACCTGGA GCAGGAAATA GGCTTTGAAA AGTTCTTTGA GGTTTATGAG 
AAAATAAAGT CTATTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGCT 
CAGAATATTT TGGGAAATGA ACATCAACAT CTTTATGCCA AGATCCTTCA TTTAGTTATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358584.1
CDS	590..4225
Translation	MDKYVRLQKIGEGSFGKAILVKSTEDGKQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGPQLIPAKRPASGQSLASVVSAQKITKPAAKYGVPVAYKKHGDKKLQEKKPLQKLKQAHQTPLKKVNPGEERGKMFEEGARKRRLGFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKATFFGGGGAVAPSFPSRGQYEHYHAIFDQMQRQRAEDNEAKWKGEIRGRRLPERQKGQLAMERAKQVEEFLQRKQEARQNKARAEGHMVYLARLRQIRLQNFNERQQIRAKLRGEKKEVDNSEGQEGSEETDMRRKKVEALKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPSGQHDSGGSPSKQQMRSVISVTSALKEVGMDSLTGTQEPAEEMQKTNNAISSKREILRRLNQNLKAQEDEKGKQCHSDTCEIIVHEDDKGHEKEKSVSSDRKKWEAGGQLVIPLDESAWDASFSATDNHTVGDVIKLDSGGSPRRVWGKSPTDSVLKILGEAELQLQTELLGNTTIRSDISSEGEKYKPLITGEEKVKCISLEMNLSATLHSSAETSSPKLSEGSPQMSLKPEGNLEERDDLETEVLQESSGANKDGSLPCTVMDVWISEIKETKETKLEDRITSQKNEDSEDGFLRNLEQLSEVHIEPGIDDSQHSYKCDTDKSIQPEPFFHKVVHSEHLNVVSQIQSILCSPEEALPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLIDVPTIGDVHQDNLEIDEIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKSIHEDEDENIEICSTIAQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011360282.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus NIMA-related kinase 1 (NEK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360282.1

ORF Insert Sequence:

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGTT TATCTTGCAA GGCTGAGACA AATAAGATTG 
CAGAATTTCA ATGAGCGCCA ACAGATTAGA GCCAAACTTC GTGGTGAAAA GAAAGAAGTG 
GATAATTCTG AAGGACAAGA GGGAAGTGAG GAGACTGACA TGAGGCGTAA AAAAGTTGAA 
GCACTTAAGG CCCAAGCAAG TGCACGTGCT TCTGTATTAA AAGAACAATT GGAACGAAAG 
AGAAAGGAAG CGTATGAGAG AGAAAAGAAA GTATGGGAAG AGCATTTGGT GGCTAAAGGA 
GTAAAGAATT CTGATATGTC TCCTTCAGGA CAGCATGATT CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTCACT TCAGCTTTGA AAGAAGTGGG TATGGACAGT 
TTAACTGGTA CCCAGGAACC CGCAGAAGAA ATGCAAAAGA CTAACAATGC TATTTCGAGT 
AAGCGAGAAA TACTACGTAG ATTAAATCAA AATCTTAAAG CTCAAGAAGA TGAAAAAGGC 
AAGCAGTGTC ACTCTGATAC CTGTGAGATA ATCGTTCACG AAGATGACAA AGGACATGAA 
AAAGAAAAGT CAGTTTCATC TGATCGCAAG AAATGGGAGG CAGGAGGGCA GCTCGTGATT 
CCTCTGGATG AGTCTGCATG GGATGCATCC TTCTCTGCAA CCGACAACCA TACAGTGGGA 
GATGTTATTA AATTAGATTC TGGAGGATCT CCAAGAAGAG TCTGGGGGAA AAGCCCTACA 
GATTCTGTTC TAAAAATACT TGGAGAAGCT GAACTACAAC TTCAGACAGA ACTATTGGGA 
AACACAACCA TTAGAAGTGA TATTTCTTCT GAAGGGGAGA AGTACAAACC CTTAATCACT 
GGAGAAGAAA AAGTAAAATG TATTTCACTT GAAATGAACC TATCAGCTAC TCTCCATTCT 
TCTGCTGAGA CAAGCAGTCC AAAGCTGAGT GAGGGATCTC CACAGATGTC ATTGAAACCA 
GAAGGAAATT TGGAAGAACG TGATGACTTG GAAACAGAAG TTTTACAAGA GTCAAGTGGA 
GCAAACAAAG ATGGTAGCTT GCCTTGCACT GTTATGGATG TGTGGATTAG TGAGATAAAA 
GAAACAAAGG AAACTAAATT GGAAGATAGG ATTACTAGTC AGAAAAATGA AGATTCTGAA 
GATGGATTTC TGAGGAATCT GGAACAACTT AGTGAAGTTC ACATAGAACC TGGAATAGAT 
GATTCTCAGC ACTCTTATAA ATGTGATACA GACAAATCTA TACAACCAGA ACCATTTTTC 
CATAAAGTGG TTCATTCCGA ACACTTGAAT GTAGTCTCTC AAATTCAGTC AATTCTGTGT 
TCACCAGAAG AAGCCTTGCC ACTTCGATCT CGCTCTGATT CACCACCAAA AAATAAAAAC 
AAGAATTCTT TGCTGATTGG ACTTTCAACT GGTCTTTTTG ATGCAAATAA CCCAAAGATG 
CTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACACTGAT AGATGTTCCT 
ACTATAGGAG ATGTACATCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAGCACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTTTTTGAAG AAACTGACAC AGATTTACAG 
GAGCTTCAGG CCTCTATGGA GCAGTTACTT AGGGAACAGC CTGGGGAAGA ATACAGTGAG 
GAGGAAGAAT CAGTCTTAAA GAACAGTGAC ATAGAGCAGA CTGCAAATGG GACAGATCTG 
GCAGATGAGG ATGACAATCC CAGTAGTGAA AGTGCTCTAA ATGAAGAATG GCACTCAGAT 
AACAGCGATG GTGAGATAAC TAGTGAATGT GAATATGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCACCTGGA GCAGGAAATA GGCTTTGAAA AGTTCTTTGA GGTTTATGAG 
AAAATAAAGT CTATTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGCT 
CAGAATATTT TGGGAAATGA ACATCAACAT CTTTATGCCA AGATCCTTCA TTTAGTTATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011360282.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358584.1 Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.

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CloneID	OPt46896
Clone ID Related Accession (Same CDS sequence)	XM_011360282.1 , XM_011360282.2
Accession Version	XM_011360282.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	serine/threonine-protein kinase Nek1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888834.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011360282.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGTT TATCTTGCAA GGCTGAGACA AATAAGATTG 
CAGAATTTCA ATGAGCGCCA ACAGATTAGA GCCAAACTTC GTGGTGAAAA GAAAGAAGTG 
GATAATTCTG AAGGACAAGA GGGAAGTGAG GAGACTGACA TGAGGCGTAA AAAAGTTGAA 
GCACTTAAGG CCCAAGCAAG TGCACGTGCT TCTGTATTAA AAGAACAATT GGAACGAAAG 
AGAAAGGAAG CGTATGAGAG AGAAAAGAAA GTATGGGAAG AGCATTTGGT GGCTAAAGGA 
GTAAAGAATT CTGATATGTC TCCTTCAGGA CAGCATGATT CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTCACT TCAGCTTTGA AAGAAGTGGG TATGGACAGT 
TTAACTGGTA CCCAGGAACC CGCAGAAGAA ATGCAAAAGA CTAACAATGC TATTTCGAGT 
AAGCGAGAAA TACTACGTAG ATTAAATCAA AATCTTAAAG CTCAAGAAGA TGAAAAAGGC 
AAGCAGTGTC ACTCTGATAC CTGTGAGATA ATCGTTCACG AAGATGACAA AGGACATGAA 
AAAGAAAAGT CAGTTTCATC TGATCGCAAG AAATGGGAGG CAGGAGGGCA GCTCGTGATT 
CCTCTGGATG AGTCTGCATG GGATGCATCC TTCTCTGCAA CCGACAACCA TACAGTGGGA 
GATGTTATTA AATTAGATTC TGGAGGATCT CCAAGAAGAG TCTGGGGGAA AAGCCCTACA 
GATTCTGTTC TAAAAATACT TGGAGAAGCT GAACTACAAC TTCAGACAGA ACTATTGGGA 
AACACAACCA TTAGAAGTGA TATTTCTTCT GAAGGGGAGA AGTACAAACC CTTAATCACT 
GGAGAAGAAA AAGTAAAATG TATTTCACTT GAAATGAACC TATCAGCTAC TCTCCATTCT 
TCTGCTGAGA CAAGCAGTCC AAAGCTGAGT GAGGGATCTC CACAGATGTC ATTGAAACCA 
GAAGGAAATT TGGAAGAACG TGATGACTTG GAAACAGAAG TTTTACAAGA GTCAAGTGGA 
GCAAACAAAG ATGGTAGCTT GCCTTGCACT GTTATGGATG TGTGGATTAG TGAGATAAAA 
GAAACAAAGG AAACTAAATT GGAAGATAGG ATTACTAGTC AGAAAAATGA AGATTCTGAA 
GATGGATTTC TGAGGAATCT GGAACAACTT AGTGAAGTTC ACATAGAACC TGGAATAGAT 
GATTCTCAGC ACTCTTATAA ATGTGATACA GACAAATCTA TACAACCAGA ACCATTTTTC 
CATAAAGTGG TTCATTCCGA ACACTTGAAT GTAGTCTCTC AAATTCAGTC AATTCTGTGT 
TCACCAGAAG AAGCCTTGCC ACTTCGATCT CGCTCTGATT CACCACCAAA AAATAAAAAC 
AAGAATTCTT TGCTGATTGG ACTTTCAACT GGTCTTTTTG ATGCAAATAA CCCAAAGATG 
CTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACACTGAT AGATGTTCCT 
ACTATAGGAG ATGTACATCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAGCACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTTTTTGAAG AAACTGACAC AGATTTACAG 
GAGCTTCAGG CCTCTATGGA GCAGTTACTT AGGGAACAGC CTGGGGAAGA ATACAGTGAG 
GAGGAAGAAT CAGTCTTAAA GAACAGTGAC ATAGAGCAGA CTGCAAATGG GACAGATCTG 
GCAGATGAGG ATGACAATCC CAGTAGTGAA AGTGCTCTAA ATGAAGAATG GCACTCAGAT 
AACAGCGATG GTGAGATAAC TAGTGAATGT GAATATGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCACCTGGA GCAGGAAATA GGCTTTGAAA AGTTCTTTGA GGTTTATGAG 
AAAATAAAGT CTATTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGCT 
CAGAATATTT TGGGAAATGA ACATCAACAT CTTTATGCCA AGATCCTTCA TTTAGTTATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358584.1
CDS	608..4243
Translation	MDKYVRLQKIGEGSFGKAILVKSTEDGKQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGPQLIPAKRPASGQSLASVVSAQKITKPAAKYGVPVAYKKHGDKKLQEKKPLQKLKQAHQTPLKKVNPGEERGKMFEEGARKRRLGFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKATFFGGGGAVAPSFPSRGQYEHYHAIFDQMQRQRAEDNEAKWKGEIRGRRLPERQKGQLAMERAKQVEEFLQRKQEARQNKARAEGHMVYLARLRQIRLQNFNERQQIRAKLRGEKKEVDNSEGQEGSEETDMRRKKVEALKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPSGQHDSGGSPSKQQMRSVISVTSALKEVGMDSLTGTQEPAEEMQKTNNAISSKREILRRLNQNLKAQEDEKGKQCHSDTCEIIVHEDDKGHEKEKSVSSDRKKWEAGGQLVIPLDESAWDASFSATDNHTVGDVIKLDSGGSPRRVWGKSPTDSVLKILGEAELQLQTELLGNTTIRSDISSEGEKYKPLITGEEKVKCISLEMNLSATLHSSAETSSPKLSEGSPQMSLKPEGNLEERDDLETEVLQESSGANKDGSLPCTVMDVWISEIKETKETKLEDRITSQKNEDSEDGFLRNLEQLSEVHIEPGIDDSQHSYKCDTDKSIQPEPFFHKVVHSEHLNVVSQIQSILCSPEEALPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLIDVPTIGDVHQDNLEIDEIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKSIHEDEDENIEICSTIAQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011360282.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360282.2

ORF Insert Sequence:

ATGGATAAAT ATGTCAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG AAAACAGTAT GTTATCAAGG AAATTAACAT TTCAAGAATG 
TCCAATAAAG AAAGGGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTACAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGCG AAGGAGGGGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAA TCCCAGAACA TATTTTTAAC CAAAGATGGG 
ACAATACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACCGT AGAACTTGCT 
CGAACCTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTTGGA TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGGTCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCCGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GACCTCAACT TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCCTGGCC 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGCGT GCCTGTGGCA 
TATAAGAAAC ATGGAGATAA AAAATTGCAG GAAAAGAAGC CACTCCAAAA ACTTAAGCAG 
GCCCATCAAA CTCCATTGAA GAAAGTGAAT CCTGGAGAAG AAAGGGGGAA AATGTTTGAG 
GAAGGAGCAA GAAAGAGAAG GTTGGGATTT ATCGAAAAAG AAAAGAAACA AAAAGATCAG 
ATTAGTTTAA TGAAGGCTGA ACAGATGAAA AGGCAAGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAT GTGCTAAGTG CTGGTGGAAG TGGTGAAGTA 
AAGGCTACCT TTTTTGGCGG TGGAGGGGCT GTGGCTCCAT CATTTCCTTC TCGAGGACAA 
TATGAACATT ATCATGCTAT TTTTGATCAA ATGCAGCGAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAGGAGAAAT ACGTGGCCGA AGGCTTCCAG AGAGGCAAAA GGGGCAGCTA 
GCTATGGAAA GAGCTAAACA AGTGGAAGAA TTCTTACAGC GAAAACAGGA AGCGAGGCAG 
AACAAAGCTC GAGCCGAAGG GCACATGGTT TATCTTGCAA GGCTGAGACA AATAAGATTG 
CAGAATTTCA ATGAGCGCCA ACAGATTAGA GCCAAACTTC GTGGTGAAAA GAAAGAAGTG 
GATAATTCTG AAGGACAAGA GGGAAGTGAG GAGACTGACA TGAGGCGTAA AAAAGTTGAA 
GCACTTAAGG CCCAAGCAAG TGCACGTGCT TCTGTATTAA AAGAACAATT GGAACGAAAG 
AGAAAGGAAG CGTATGAGAG AGAAAAGAAA GTATGGGAAG AGCATTTGGT GGCTAAAGGA 
GTAAAGAATT CTGATATGTC TCCTTCAGGA CAGCATGATT CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTCACT TCAGCTTTGA AAGAAGTGGG TATGGACAGT 
TTAACTGGTA CCCAGGAACC CGCAGAAGAA ATGCAAAAGA CTAACAATGC TATTTCGAGT 
AAGCGAGAAA TACTACGTAG ATTAAATCAA AATCTTAAAG CTCAAGAAGA TGAAAAAGGC 
AAGCAGTGTC ACTCTGATAC CTGTGAGATA ATCGTTCACG AAGATGACAA AGGACATGAA 
AAAGAAAAGT CAGTTTCATC TGATCGCAAG AAATGGGAGG CAGGAGGGCA GCTCGTGATT 
CCTCTGGATG AGTCTGCATG GGATGCATCC TTCTCTGCAA CCGACAACCA TACAGTGGGA 
GATGTTATTA AATTAGATTC TGGAGGATCT CCAAGAAGAG TCTGGGGGAA AAGCCCTACA 
GATTCTGTTC TAAAAATACT TGGAGAAGCT GAACTACAAC TTCAGACAGA ACTATTGGGA 
AACACAACCA TTAGAAGTGA TATTTCTTCT GAAGGGGAGA AGTACAAACC CTTAATCACT 
GGAGAAGAAA AAGTAAAATG TATTTCACTT GAAATGAACC TATCAGCTAC TCTCCATTCT 
TCTGCTGAGA CAAGCAGTCC AAAGCTGAGT GAGGGATCTC CACAGATGTC ATTGAAACCA 
GAAGGAAATT TGGAAGAACG TGATGACTTG GAAACAGAAG TTTTACAAGA GTCAAGTGGA 
GCAAACAAAG ATGGTAGCTT GCCTTGCACT GTTATGGATG TGTGGATTAG TGAGATAAAA 
GAAACAAAGG AAACTAAATT GGAAGATAGG ATTACTAGTC AGAAAAATGA AGATTCTGAA 
GATGGATTTC TGAGGAATCT GGAACAACTT AGTGAAGTTC ACATAGAACC TGGAATAGAT 
GATTCTCAGC ACTCTTATAA ATGTGATACA GACAAATCTA TACAACCAGA ACCATTTTTC 
CATAAAGTGG TTCATTCCGA ACACTTGAAT GTAGTCTCTC AAATTCAGTC AATTCTGTGT 
TCACCAGAAG AAGCCTTGCC ACTTCGATCT CGCTCTGATT CACCACCAAA AAATAAAAAC 
AAGAATTCTT TGCTGATTGG ACTTTCAACT GGTCTTTTTG ATGCAAATAA CCCAAAGATG 
CTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACACTGAT AGATGTTCCT 
ACTATAGGAG ATGTACATCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAGCACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTTTTTGAAG AAACTGACAC AGATTTACAG 
GAGCTTCAGG CCTCTATGGA GCAGTTACTT AGGGAACAGC CTGGGGAAGA ATACAGTGAG 
GAGGAAGAAT CAGTCTTAAA GAACAGTGAC ATAGAGCAGA CTGCAAATGG GACAGATCTG 
GCAGATGAGG ATGACAATCC CAGTAGTGAA AGTGCTCTAA ATGAAGAATG GCACTCAGAT 
AACAGCGATG GTGAGATAAC TAGTGAATGT GAATATGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCACCTGGA GCAGGAAATA GGCTTTGAAA AGTTCTTTGA GGTTTATGAG 
AAAATAAAGT CTATTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGCT 
CAGAATATTT TGGGAAATGA ACATCAACAT CTTTATGCCA AGATCCTTCA TTTAGTTATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NEK1
Entrez Gene ID	105292164
Full Name	NIMA related kinase 1
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NEK1 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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