NOS2 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS2
Entrez Gene ID	105291934
Full Name	nitric oxide synthase 2
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011360001.1	XP_011358303.1	nitric oxide synthase, inducible
XM_011360001.2	XP_011358303.1	nitric oxide synthase, inducible

Pathways from BioSystems
Gene Ontology

The following NOS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt46688	XM_011360001.2 Latest version!	Pteropus vampyrus nitric oxide synthase 2 (NOS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OPt46688	XM_011360001.1	Pteropus vampyrus nitric oxide synthase 2, inducible (NOS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS2 ( XM_011360001.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358303.1 Pteropus vampyrus nitric oxide synthase 2, inducible (NOS2), mRNA.

Price & Availability↑

CloneID	OPt46688
Clone ID Related Accession (Same CDS sequence)	XM_011360001.1 , XM_011360001.2
Accession Version	XM_011360001.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	nitric oxide synthase, inducible
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888831.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC TTTGGAAGTT TCTGTTCAAG GCCAGATCAT ACCAGTATGA CTTGACGAAG 
GAACGAGACA TCAACAACAA CATGGGGAAA GCCATCCAGG CTCCCTCCAG TCCAGTGACG 
CAGGATGACA CCAAGCGCCA CAGCCTCGGC AAGCACCAGA ATGAGGACCC CCAGCCCTTC 
CTGGGGACAG TAAAGAAGTC TCCAGAATCC CTGGTCAAGC TGGGTGCACC CCCATCGACC 
TGCCCACGGC ACGTGAGGAT CAAAAACTGG GGCAGTGGGG TGACTTTCCA AGATACACTT 
CACCACAAAG CCAAAGGGGA TCTTGCTTGC AAGTCCAAGT GTTGCCTGGG AGCCATCATG 
AACCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCTCCCCAGA CGAGCTTCTA 
CCTCAAGCTA TCGAGTTTGT CAACCAGTAT TATAGCTCCT TCAAAGAGTC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAG 
CTGACGGGAG ATGAGCTCAT CTTTGCCACC AAGCAGGCCT GGCGCAATGC CCCCCGCTGC 
ATCGGGAGGA TCCAGTGGTC CAACCTACAG GTCTTCGATG CCCGGAGCTG TTCTACTGCC 
CAGGAAATGT TCGAGCATAT CTGCAGACAC CTGCGCTATG CCACCAACAA TGGCAACATC 
AGGTCGGCCA TCACCGTGTT CCCTCAGCGG AGTGATGGGA AACATGACTT CCGGGTCTGG 
AATTCGCAGC TCATCCGGTA TGCTGGTTAC CAGATGCCTG ATGGTACCAT CAGAGGGGAC 
CCTGCCTGCG TGGAGTTCAC CCAGCTGTGC ATCGACCTGG GCTGGAAGCC GAAGTACGGC 
CGCTTTGATG TGTTGCCTCT GGTCCTGCAG GCTGATGGCC ACGACCCTGA GTTCTTTGAA 
ATCCCGCCTG ACCTTGTGCT CGAGGTGCCC ATGGAACATC CCAAGTACGA GTGGTTCCAG 
GAACTGGAGC TCAAGTGGTA TGCCCTGCCT GCCGTGTCCA ATATGCTGCT TGAGGTGGGT 
GGCCTTGAGT TCCCAGGGTG CCCTTTCAAC GGCTGGTACA TGGGCACAGA GATCGGGGTC 
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC 
CTAGAAACAC ACAAGTTGAC CTCACTCTGG AAAGACCAGG CCGTCATTGA GATCAACGTC 
GCTGTGCTCC ACAGTTTCCA GAAGCAAAAT GTGACCATCA TGGATCACCA CTCTGCTGCA 
GAGTCCTTCA TGAAGTACAT GCAGAATGAG TACCGGTCCC GAGGGGGCTG CCCAGCCGAC 
TGGGTTTGGC TGGTCCCCCC AATTTCTGGA AGCATCACTC CCGTGTTCCA CCAGGAGATG 
TTAAACTATG TGCTGTCCCC TTTCTACTAC TACCAGGTGG AGCCCTGGAA AACCCATGTC 
TGGCAGGATG AGAAGCGGAG ACCCCAGAGA AGAGAGATTC GATTCCAAGT CTTGGTTAAA 
GCCGTGCTGT TCACCTCCAT GCTGATGCGC AAGACCATGG CATCCCGAGT CAGAGCCACA 
GTCCTTTTTG CGACGGAGAC AGGAAAATCA GAAACCCTGG CCCGGGACCT AGGGGCCTTG 
TTCAGCTGTG CCTTCAACCC CAAGGTTCTC TGCATGGATG AGTATAGGCT GAGCAGCCTG 
GAGGAGGAGC AGCTGCTTCT GGTGGTGACC AGTACATTTG GGAACGGAGA CTCCCCTGGC 
AATGGAGAGA AACTGAAGAA ATCCCTCTTC ATGCTGAAAG AGCTCACCAA CAAGTTCAGG 
TATGCTGTGT TTGGCCTCGG TTCTAGCATG TACCCTCAGT TCTGTGCCTT TGCTCATGAT 
ATCGACCAGA AGTTGTCCCA CCTGGGAGCC CCTCAGCTCA CCCCAACAGG GGAAGGGGAC 
GAACTCAGTG GGCAGGAGGA GGCCTTCCGC AGCTGGGCTG TGCAAACCTT CAAGGCAGCC 
TGTGAGACAT TCAACGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCACC 
AATGTGACCT GGGACCCGAA CCACTACAGG CTTGTGCAGG ACTCACAGCC TTTGGACCTC 
AACAAAGCCC TCAGCAGTAT GCACGCCAAG AATGTGTTCA CCTTGAAGCT CAAATCGCAG 
CAGAATCTAC AGAGTCGGAA GTCCAGCCGC ACCACCCTCC TGGTGGAACT CTCCTGCGAG 
GGCAGTCAAG ATCTCAGCTA CCTGCCTGGA GAGCACCTGG GGGTTTTCCC AAGCAACCAG 
CTGGCCCTGG TCCAAGGCAT TTTGGAGCGA GTGGTGGACA GCCCCGCACC CCACCAACCC 
ATGCGCCTGG AGAACCTCAG TGAGACCGGC AGCTACTGGG TCAGGGACAA GCGGCTGCCC 
CCCTGCTCAC TCAACCAAGC CCTCACCTAC TTCCTGGATA TCACCACCCC ACCAACTCAA 
CTGCTGCTCC GAAAGCTGGC CCAGTTGGCC CTGGAAGAGG CCGAGAGGCA GAAGCTAGAA 
GCACTGTGCC AGCCCTCAGA ATATAATAAG TGGAAGTTCA CCAACAGCCC CACGTTCTTG 
GAGGTGCTCG AGGAGTTCCC ATCCCTGCGG GTGTCTGCCA CCTTCCTGCT CTCTCAGCTC 
CCTATTCTGA AACCGCGGTA CTACTCCATC AGCTCCTCCA GGGACCACAC ACCCACGGAG 
GTCCACCTCA CCGTGGCCGT GCTCACCTAC CGCACCCAAG ACGGCCGAGG TCCCCTACAC 
CACGGTGTCT GCAGCACATG GCTTAGCAGC CTGAAGCCCC AAGACTCAGT GCCCTGCTTT 
GTACGAAGTG CCAACGGCTT CCAGCTTCCT GAGGACCCCT CCCATCCTTG CATCCTCATC 
GGGCCTGGCA CGGGCATCGC TCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGATGCT 
GAGCACAAAG GGCTCCAGGG CGGCCGCATG ATCCTGTTGT TTGGGTGCCG GCACCCAGAC 
GAGGACCACC TCTATCAGGA GGAGATGCTG GAGATGGCCC GGAAGGGGGT GCTGCATGGG 
GTGCACACAG CCTATTCTCG CCTACCTGGC CAGCCCAAGG TCTACGTTCA AGACATCCTG 
CAGCAGCAGC TGGCCGGTGA GGTGCTTCGC GTGCTCCACG AGGAGCAAGG CCACCTCTAT 
GTCTGCGGGG ACGTGCGCAT GGCCCGGGAT GTGGCCCACA CCCTGAAGAA GCTGGTGGCT 
GCCAAACTGA GCCTGAGTGA GGAGCAGGTC GAGGACTATT TCTTCCAGCT TAAGAGCCAG 
AAGCGCTATC ATGAAGATAT CTTTGGTGCT GTATTTCCCT ACGAGGTAAA AAAGGATGGG 
GCAGCAGGGC AGCCCAGTAA TATCAGAGCT CCAACAGACC AGAGGAGAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358303.1
CDS	67..3540
Translation	MASLWKFLFKARSYQYDLTKERDINNNMGKAIQAPSSPVTQDDTKRHSLGKHQNEDPQPFLGTVKKSPESLVKLGAPPSTCPRHVRIKNWGSGVTFQDTLHHKAKGDLACKSKCCLGAIMNPKSLTRGPRDKPTSPDELLPQAIEFVNQYYSSFKESKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAQEMFEHICRHLRYATNNGNIRSAITVFPQRSDGKHDFRVWNSQLIRYAGYQMPDGTIRGDPACVEFTQLCIDLGWKPKYGRFDVLPLVLQADGHDPEFFEIPPDLVLEVPMEHPKYEWFQELELKWYALPAVSNMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLTSLWKDQAVIEINVAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWVWLVPPISGSITPVFHQEMLNYVLSPFYYYQVEPWKTHVWQDEKRRPQRREIRFQVLVKAVLFTSMLMRKTMASRVRATVLFATETGKSETLARDLGALFSCAFNPKVLCMDEYRLSSLEEEQLLLVVTSTFGNGDSPGNGEKLKKSLFMLKELTNKFRYAVFGLGSSMYPQFCAFAHDIDQKLSHLGAPQLTPTGEGDELSGQEEAFRSWAVQTFKAACETFNVRGKQHIQIPKLYTTNVTWDPNHYRLVQDSQPLDLNKALSSMHAKNVFTLKLKSQQNLQSRKSSRTTLLVELSCEGSQDLSYLPGEHLGVFPSNQLALVQGILERVVDSPAPHQPMRLENLSETGSYWVRDKRLPPCSLNQALTYFLDITTPPTQLLLRKLAQLALEEAERQKLEALCQPSEYNKWKFTNSPTFLEVLEEFPSLRVSATFLLSQLPILKPRYYSISSSRDHTPTEVHLTVAVLTYRTQDGRGPLHHGVCSTWLSSLKPQDSVPCFVRSANGFQLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDAEHKGLQGGRMILLFGCRHPDEDHLYQEEMLEMARKGVLHGVHTAYSRLPGQPKVYVQDILQQQLAGEVLRVLHEEQGHLYVCGDVRMARDVAHTLKKLVAAKLSLSEEQVEDYFFQLKSQKRYHEDIFGAVFPYEVKKDGAAGQPSNIRAPTDQRRI

Target ORF information:

RefSeq Version	XM_011360001.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus nitric oxide synthase 2, inducible (NOS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360001.1

ORF Insert Sequence:

ATGGCCTCTC TTTGGAAGTT TCTGTTCAAG GCCAGATCAT ACCAGTATGA CTTGACGAAG 
GAACGAGACA TCAACAACAA CATGGGGAAA GCCATCCAGG CTCCCTCCAG TCCAGTGACG 
CAGGATGACA CCAAGCGCCA CAGCCTCGGC AAGCACCAGA ATGAGGACCC CCAGCCCTTC 
CTGGGGACAG TAAAGAAGTC TCCAGAATCC CTGGTCAAGC TGGGTGCACC CCCATCGACC 
TGCCCACGGC ACGTGAGGAT CAAAAACTGG GGCAGTGGGG TGACTTTCCA AGATACACTT 
CACCACAAAG CCAAAGGGGA TCTTGCTTGC AAGTCCAAGT GTTGCCTGGG AGCCATCATG 
AACCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCTCCCCAGA CGAGCTTCTA 
CCTCAAGCTA TCGAGTTTGT CAACCAGTAT TATAGCTCCT TCAAAGAGTC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAG 
CTGACGGGAG ATGAGCTCAT CTTTGCCACC AAGCAGGCCT GGCGCAATGC CCCCCGCTGC 
ATCGGGAGGA TCCAGTGGTC CAACCTACAG GTCTTCGATG CCCGGAGCTG TTCTACTGCC 
CAGGAAATGT TCGAGCATAT CTGCAGACAC CTGCGCTATG CCACCAACAA TGGCAACATC 
AGGTCGGCCA TCACCGTGTT CCCTCAGCGG AGTGATGGGA AACATGACTT CCGGGTCTGG 
AATTCGCAGC TCATCCGGTA TGCTGGTTAC CAGATGCCTG ATGGTACCAT CAGAGGGGAC 
CCTGCCTGCG TGGAGTTCAC CCAGCTGTGC ATCGACCTGG GCTGGAAGCC GAAGTACGGC 
CGCTTTGATG TGTTGCCTCT GGTCCTGCAG GCTGATGGCC ACGACCCTGA GTTCTTTGAA 
ATCCCGCCTG ACCTTGTGCT CGAGGTGCCC ATGGAACATC CCAAGTACGA GTGGTTCCAG 
GAACTGGAGC TCAAGTGGTA TGCCCTGCCT GCCGTGTCCA ATATGCTGCT TGAGGTGGGT 
GGCCTTGAGT TCCCAGGGTG CCCTTTCAAC GGCTGGTACA TGGGCACAGA GATCGGGGTC 
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC 
CTAGAAACAC ACAAGTTGAC CTCACTCTGG AAAGACCAGG CCGTCATTGA GATCAACGTC 
GCTGTGCTCC ACAGTTTCCA GAAGCAAAAT GTGACCATCA TGGATCACCA CTCTGCTGCA 
GAGTCCTTCA TGAAGTACAT GCAGAATGAG TACCGGTCCC GAGGGGGCTG CCCAGCCGAC 
TGGGTTTGGC TGGTCCCCCC AATTTCTGGA AGCATCACTC CCGTGTTCCA CCAGGAGATG 
TTAAACTATG TGCTGTCCCC TTTCTACTAC TACCAGGTGG AGCCCTGGAA AACCCATGTC 
TGGCAGGATG AGAAGCGGAG ACCCCAGAGA AGAGAGATTC GATTCCAAGT CTTGGTTAAA 
GCCGTGCTGT TCACCTCCAT GCTGATGCGC AAGACCATGG CATCCCGAGT CAGAGCCACA 
GTCCTTTTTG CGACGGAGAC AGGAAAATCA GAAACCCTGG CCCGGGACCT AGGGGCCTTG 
TTCAGCTGTG CCTTCAACCC CAAGGTTCTC TGCATGGATG AGTATAGGCT GAGCAGCCTG 
GAGGAGGAGC AGCTGCTTCT GGTGGTGACC AGTACATTTG GGAACGGAGA CTCCCCTGGC 
AATGGAGAGA AACTGAAGAA ATCCCTCTTC ATGCTGAAAG AGCTCACCAA CAAGTTCAGG 
TATGCTGTGT TTGGCCTCGG TTCTAGCATG TACCCTCAGT TCTGTGCCTT TGCTCATGAT 
ATCGACCAGA AGTTGTCCCA CCTGGGAGCC CCTCAGCTCA CCCCAACAGG GGAAGGGGAC 
GAACTCAGTG GGCAGGAGGA GGCCTTCCGC AGCTGGGCTG TGCAAACCTT CAAGGCAGCC 
TGTGAGACAT TCAACGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCACC 
AATGTGACCT GGGACCCGAA CCACTACAGG CTTGTGCAGG ACTCACAGCC TTTGGACCTC 
AACAAAGCCC TCAGCAGTAT GCACGCCAAG AATGTGTTCA CCTTGAAGCT CAAATCGCAG 
CAGAATCTAC AGAGTCGGAA GTCCAGCCGC ACCACCCTCC TGGTGGAACT CTCCTGCGAG 
GGCAGTCAAG ATCTCAGCTA CCTGCCTGGA GAGCACCTGG GGGTTTTCCC AAGCAACCAG 
CTGGCCCTGG TCCAAGGCAT TTTGGAGCGA GTGGTGGACA GCCCCGCACC CCACCAACCC 
ATGCGCCTGG AGAACCTCAG TGAGACCGGC AGCTACTGGG TCAGGGACAA GCGGCTGCCC 
CCCTGCTCAC TCAACCAAGC CCTCACCTAC TTCCTGGATA TCACCACCCC ACCAACTCAA 
CTGCTGCTCC GAAAGCTGGC CCAGTTGGCC CTGGAAGAGG CCGAGAGGCA GAAGCTAGAA 
GCACTGTGCC AGCCCTCAGA ATATAATAAG TGGAAGTTCA CCAACAGCCC CACGTTCTTG 
GAGGTGCTCG AGGAGTTCCC ATCCCTGCGG GTGTCTGCCA CCTTCCTGCT CTCTCAGCTC 
CCTATTCTGA AACCGCGGTA CTACTCCATC AGCTCCTCCA GGGACCACAC ACCCACGGAG 
GTCCACCTCA CCGTGGCCGT GCTCACCTAC CGCACCCAAG ACGGCCGAGG TCCCCTACAC 
CACGGTGTCT GCAGCACATG GCTTAGCAGC CTGAAGCCCC AAGACTCAGT GCCCTGCTTT 
GTACGAAGTG CCAACGGCTT CCAGCTTCCT GAGGACCCCT CCCATCCTTG CATCCTCATC 
GGGCCTGGCA CGGGCATCGC TCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGATGCT 
GAGCACAAAG GGCTCCAGGG CGGCCGCATG ATCCTGTTGT TTGGGTGCCG GCACCCAGAC 
GAGGACCACC TCTATCAGGA GGAGATGCTG GAGATGGCCC GGAAGGGGGT GCTGCATGGG 
GTGCACACAG CCTATTCTCG CCTACCTGGC CAGCCCAAGG TCTACGTTCA AGACATCCTG 
CAGCAGCAGC TGGCCGGTGA GGTGCTTCGC GTGCTCCACG AGGAGCAAGG CCACCTCTAT 
GTCTGCGGGG ACGTGCGCAT GGCCCGGGAT GTGGCCCACA CCCTGAAGAA GCTGGTGGCT 
GCCAAACTGA GCCTGAGTGA GGAGCAGGTC GAGGACTATT TCTTCCAGCT TAAGAGCCAG 
AAGCGCTATC ATGAAGATAT CTTTGGTGCT GTATTTCCCT ACGAGGTAAA AAAGGATGGG 
GCAGCAGGGC AGCCCAGTAA TATCAGAGCT CCAACAGACC AGAGGAGAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_011360001.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358303.1 Pteropus vampyrus nitric oxide synthase 2 (NOS2), mRNA.

Price & Availability↑

CloneID	OPt46688
Clone ID Related Accession (Same CDS sequence)	XM_011360001.1 , XM_011360001.2
Accession Version	XM_011360001.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	nitric oxide synthase, inducible
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888831.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011360001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC TTTGGAAGTT TCTGTTCAAG GCCAGATCAT ACCAGTATGA CTTGACGAAG 
GAACGAGACA TCAACAACAA CATGGGGAAA GCCATCCAGG CTCCCTCCAG TCCAGTGACG 
CAGGATGACA CCAAGCGCCA CAGCCTCGGC AAGCACCAGA ATGAGGACCC CCAGCCCTTC 
CTGGGGACAG TAAAGAAGTC TCCAGAATCC CTGGTCAAGC TGGGTGCACC CCCATCGACC 
TGCCCACGGC ACGTGAGGAT CAAAAACTGG GGCAGTGGGG TGACTTTCCA AGATACACTT 
CACCACAAAG CCAAAGGGGA TCTTGCTTGC AAGTCCAAGT GTTGCCTGGG AGCCATCATG 
AACCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCTCCCCAGA CGAGCTTCTA 
CCTCAAGCTA TCGAGTTTGT CAACCAGTAT TATAGCTCCT TCAAAGAGTC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAG 
CTGACGGGAG ATGAGCTCAT CTTTGCCACC AAGCAGGCCT GGCGCAATGC CCCCCGCTGC 
ATCGGGAGGA TCCAGTGGTC CAACCTACAG GTCTTCGATG CCCGGAGCTG TTCTACTGCC 
CAGGAAATGT TCGAGCATAT CTGCAGACAC CTGCGCTATG CCACCAACAA TGGCAACATC 
AGGTCGGCCA TCACCGTGTT CCCTCAGCGG AGTGATGGGA AACATGACTT CCGGGTCTGG 
AATTCGCAGC TCATCCGGTA TGCTGGTTAC CAGATGCCTG ATGGTACCAT CAGAGGGGAC 
CCTGCCTGCG TGGAGTTCAC CCAGCTGTGC ATCGACCTGG GCTGGAAGCC GAAGTACGGC 
CGCTTTGATG TGTTGCCTCT GGTCCTGCAG GCTGATGGCC ACGACCCTGA GTTCTTTGAA 
ATCCCGCCTG ACCTTGTGCT CGAGGTGCCC ATGGAACATC CCAAGTACGA GTGGTTCCAG 
GAACTGGAGC TCAAGTGGTA TGCCCTGCCT GCCGTGTCCA ATATGCTGCT TGAGGTGGGT 
GGCCTTGAGT TCCCAGGGTG CCCTTTCAAC GGCTGGTACA TGGGCACAGA GATCGGGGTC 
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC 
CTAGAAACAC ACAAGTTGAC CTCACTCTGG AAAGACCAGG CCGTCATTGA GATCAACGTC 
GCTGTGCTCC ACAGTTTCCA GAAGCAAAAT GTGACCATCA TGGATCACCA CTCTGCTGCA 
GAGTCCTTCA TGAAGTACAT GCAGAATGAG TACCGGTCCC GAGGGGGCTG CCCAGCCGAC 
TGGGTTTGGC TGGTCCCCCC AATTTCTGGA AGCATCACTC CCGTGTTCCA CCAGGAGATG 
TTAAACTATG TGCTGTCCCC TTTCTACTAC TACCAGGTGG AGCCCTGGAA AACCCATGTC 
TGGCAGGATG AGAAGCGGAG ACCCCAGAGA AGAGAGATTC GATTCCAAGT CTTGGTTAAA 
GCCGTGCTGT TCACCTCCAT GCTGATGCGC AAGACCATGG CATCCCGAGT CAGAGCCACA 
GTCCTTTTTG CGACGGAGAC AGGAAAATCA GAAACCCTGG CCCGGGACCT AGGGGCCTTG 
TTCAGCTGTG CCTTCAACCC CAAGGTTCTC TGCATGGATG AGTATAGGCT GAGCAGCCTG 
GAGGAGGAGC AGCTGCTTCT GGTGGTGACC AGTACATTTG GGAACGGAGA CTCCCCTGGC 
AATGGAGAGA AACTGAAGAA ATCCCTCTTC ATGCTGAAAG AGCTCACCAA CAAGTTCAGG 
TATGCTGTGT TTGGCCTCGG TTCTAGCATG TACCCTCAGT TCTGTGCCTT TGCTCATGAT 
ATCGACCAGA AGTTGTCCCA CCTGGGAGCC CCTCAGCTCA CCCCAACAGG GGAAGGGGAC 
GAACTCAGTG GGCAGGAGGA GGCCTTCCGC AGCTGGGCTG TGCAAACCTT CAAGGCAGCC 
TGTGAGACAT TCAACGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCACC 
AATGTGACCT GGGACCCGAA CCACTACAGG CTTGTGCAGG ACTCACAGCC TTTGGACCTC 
AACAAAGCCC TCAGCAGTAT GCACGCCAAG AATGTGTTCA CCTTGAAGCT CAAATCGCAG 
CAGAATCTAC AGAGTCGGAA GTCCAGCCGC ACCACCCTCC TGGTGGAACT CTCCTGCGAG 
GGCAGTCAAG ATCTCAGCTA CCTGCCTGGA GAGCACCTGG GGGTTTTCCC AAGCAACCAG 
CTGGCCCTGG TCCAAGGCAT TTTGGAGCGA GTGGTGGACA GCCCCGCACC CCACCAACCC 
ATGCGCCTGG AGAACCTCAG TGAGACCGGC AGCTACTGGG TCAGGGACAA GCGGCTGCCC 
CCCTGCTCAC TCAACCAAGC CCTCACCTAC TTCCTGGATA TCACCACCCC ACCAACTCAA 
CTGCTGCTCC GAAAGCTGGC CCAGTTGGCC CTGGAAGAGG CCGAGAGGCA GAAGCTAGAA 
GCACTGTGCC AGCCCTCAGA ATATAATAAG TGGAAGTTCA CCAACAGCCC CACGTTCTTG 
GAGGTGCTCG AGGAGTTCCC ATCCCTGCGG GTGTCTGCCA CCTTCCTGCT CTCTCAGCTC 
CCTATTCTGA AACCGCGGTA CTACTCCATC AGCTCCTCCA GGGACCACAC ACCCACGGAG 
GTCCACCTCA CCGTGGCCGT GCTCACCTAC CGCACCCAAG ACGGCCGAGG TCCCCTACAC 
CACGGTGTCT GCAGCACATG GCTTAGCAGC CTGAAGCCCC AAGACTCAGT GCCCTGCTTT 
GTACGAAGTG CCAACGGCTT CCAGCTTCCT GAGGACCCCT CCCATCCTTG CATCCTCATC 
GGGCCTGGCA CGGGCATCGC TCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGATGCT 
GAGCACAAAG GGCTCCAGGG CGGCCGCATG ATCCTGTTGT TTGGGTGCCG GCACCCAGAC 
GAGGACCACC TCTATCAGGA GGAGATGCTG GAGATGGCCC GGAAGGGGGT GCTGCATGGG 
GTGCACACAG CCTATTCTCG CCTACCTGGC CAGCCCAAGG TCTACGTTCA AGACATCCTG 
CAGCAGCAGC TGGCCGGTGA GGTGCTTCGC GTGCTCCACG AGGAGCAAGG CCACCTCTAT 
GTCTGCGGGG ACGTGCGCAT GGCCCGGGAT GTGGCCCACA CCCTGAAGAA GCTGGTGGCT 
GCCAAACTGA GCCTGAGTGA GGAGCAGGTC GAGGACTATT TCTTCCAGCT TAAGAGCCAG 
AAGCGCTATC ATGAAGATAT CTTTGGTGCT GTATTTCCCT ACGAGGTAAA AAAGGATGGG 
GCAGCAGGGC AGCCCAGTAA TATCAGAGCT CCAACAGACC AGAGGAGAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358303.1
CDS	67..3540
Translation	MASLWKFLFKARSYQYDLTKERDINNNMGKAIQAPSSPVTQDDTKRHSLGKHQNEDPQPFLGTVKKSPESLVKLGAPPSTCPRHVRIKNWGSGVTFQDTLHHKAKGDLACKSKCCLGAIMNPKSLTRGPRDKPTSPDELLPQAIEFVNQYYSSFKESKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAQEMFEHICRHLRYATNNGNIRSAITVFPQRSDGKHDFRVWNSQLIRYAGYQMPDGTIRGDPACVEFTQLCIDLGWKPKYGRFDVLPLVLQADGHDPEFFEIPPDLVLEVPMEHPKYEWFQELELKWYALPAVSNMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLTSLWKDQAVIEINVAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWVWLVPPISGSITPVFHQEMLNYVLSPFYYYQVEPWKTHVWQDEKRRPQRREIRFQVLVKAVLFTSMLMRKTMASRVRATVLFATETGKSETLARDLGALFSCAFNPKVLCMDEYRLSSLEEEQLLLVVTSTFGNGDSPGNGEKLKKSLFMLKELTNKFRYAVFGLGSSMYPQFCAFAHDIDQKLSHLGAPQLTPTGEGDELSGQEEAFRSWAVQTFKAACETFNVRGKQHIQIPKLYTTNVTWDPNHYRLVQDSQPLDLNKALSSMHAKNVFTLKLKSQQNLQSRKSSRTTLLVELSCEGSQDLSYLPGEHLGVFPSNQLALVQGILERVVDSPAPHQPMRLENLSETGSYWVRDKRLPPCSLNQALTYFLDITTPPTQLLLRKLAQLALEEAERQKLEALCQPSEYNKWKFTNSPTFLEVLEEFPSLRVSATFLLSQLPILKPRYYSISSSRDHTPTEVHLTVAVLTYRTQDGRGPLHHGVCSTWLSSLKPQDSVPCFVRSANGFQLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDAEHKGLQGGRMILLFGCRHPDEDHLYQEEMLEMARKGVLHGVHTAYSRLPGQPKVYVQDILQQQLAGEVLRVLHEEQGHLYVCGDVRMARDVAHTLKKLVAAKLSLSEEQVEDYFFQLKSQKRYHEDIFGAVFPYEVKKDGAAGQPSNIRAPTDQRRI

Target ORF information:

RefSeq Version	XM_011360001.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus nitric oxide synthase 2 (NOS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011360001.2

ORF Insert Sequence:

ATGGCCTCTC TTTGGAAGTT TCTGTTCAAG GCCAGATCAT ACCAGTATGA CTTGACGAAG 
GAACGAGACA TCAACAACAA CATGGGGAAA GCCATCCAGG CTCCCTCCAG TCCAGTGACG 
CAGGATGACA CCAAGCGCCA CAGCCTCGGC AAGCACCAGA ATGAGGACCC CCAGCCCTTC 
CTGGGGACAG TAAAGAAGTC TCCAGAATCC CTGGTCAAGC TGGGTGCACC CCCATCGACC 
TGCCCACGGC ACGTGAGGAT CAAAAACTGG GGCAGTGGGG TGACTTTCCA AGATACACTT 
CACCACAAAG CCAAAGGGGA TCTTGCTTGC AAGTCCAAGT GTTGCCTGGG AGCCATCATG 
AACCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCTCCCCAGA CGAGCTTCTA 
CCTCAAGCTA TCGAGTTTGT CAACCAGTAT TATAGCTCCT TCAAAGAGTC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAG 
CTGACGGGAG ATGAGCTCAT CTTTGCCACC AAGCAGGCCT GGCGCAATGC CCCCCGCTGC 
ATCGGGAGGA TCCAGTGGTC CAACCTACAG GTCTTCGATG CCCGGAGCTG TTCTACTGCC 
CAGGAAATGT TCGAGCATAT CTGCAGACAC CTGCGCTATG CCACCAACAA TGGCAACATC 
AGGTCGGCCA TCACCGTGTT CCCTCAGCGG AGTGATGGGA AACATGACTT CCGGGTCTGG 
AATTCGCAGC TCATCCGGTA TGCTGGTTAC CAGATGCCTG ATGGTACCAT CAGAGGGGAC 
CCTGCCTGCG TGGAGTTCAC CCAGCTGTGC ATCGACCTGG GCTGGAAGCC GAAGTACGGC 
CGCTTTGATG TGTTGCCTCT GGTCCTGCAG GCTGATGGCC ACGACCCTGA GTTCTTTGAA 
ATCCCGCCTG ACCTTGTGCT CGAGGTGCCC ATGGAACATC CCAAGTACGA GTGGTTCCAG 
GAACTGGAGC TCAAGTGGTA TGCCCTGCCT GCCGTGTCCA ATATGCTGCT TGAGGTGGGT 
GGCCTTGAGT TCCCAGGGTG CCCTTTCAAC GGCTGGTACA TGGGCACAGA GATCGGGGTC 
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC 
CTAGAAACAC ACAAGTTGAC CTCACTCTGG AAAGACCAGG CCGTCATTGA GATCAACGTC 
GCTGTGCTCC ACAGTTTCCA GAAGCAAAAT GTGACCATCA TGGATCACCA CTCTGCTGCA 
GAGTCCTTCA TGAAGTACAT GCAGAATGAG TACCGGTCCC GAGGGGGCTG CCCAGCCGAC 
TGGGTTTGGC TGGTCCCCCC AATTTCTGGA AGCATCACTC CCGTGTTCCA CCAGGAGATG 
TTAAACTATG TGCTGTCCCC TTTCTACTAC TACCAGGTGG AGCCCTGGAA AACCCATGTC 
TGGCAGGATG AGAAGCGGAG ACCCCAGAGA AGAGAGATTC GATTCCAAGT CTTGGTTAAA 
GCCGTGCTGT TCACCTCCAT GCTGATGCGC AAGACCATGG CATCCCGAGT CAGAGCCACA 
GTCCTTTTTG CGACGGAGAC AGGAAAATCA GAAACCCTGG CCCGGGACCT AGGGGCCTTG 
TTCAGCTGTG CCTTCAACCC CAAGGTTCTC TGCATGGATG AGTATAGGCT GAGCAGCCTG 
GAGGAGGAGC AGCTGCTTCT GGTGGTGACC AGTACATTTG GGAACGGAGA CTCCCCTGGC 
AATGGAGAGA AACTGAAGAA ATCCCTCTTC ATGCTGAAAG AGCTCACCAA CAAGTTCAGG 
TATGCTGTGT TTGGCCTCGG TTCTAGCATG TACCCTCAGT TCTGTGCCTT TGCTCATGAT 
ATCGACCAGA AGTTGTCCCA CCTGGGAGCC CCTCAGCTCA CCCCAACAGG GGAAGGGGAC 
GAACTCAGTG GGCAGGAGGA GGCCTTCCGC AGCTGGGCTG TGCAAACCTT CAAGGCAGCC 
TGTGAGACAT TCAACGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCACC 
AATGTGACCT GGGACCCGAA CCACTACAGG CTTGTGCAGG ACTCACAGCC TTTGGACCTC 
AACAAAGCCC TCAGCAGTAT GCACGCCAAG AATGTGTTCA CCTTGAAGCT CAAATCGCAG 
CAGAATCTAC AGAGTCGGAA GTCCAGCCGC ACCACCCTCC TGGTGGAACT CTCCTGCGAG 
GGCAGTCAAG ATCTCAGCTA CCTGCCTGGA GAGCACCTGG GGGTTTTCCC AAGCAACCAG 
CTGGCCCTGG TCCAAGGCAT TTTGGAGCGA GTGGTGGACA GCCCCGCACC CCACCAACCC 
ATGCGCCTGG AGAACCTCAG TGAGACCGGC AGCTACTGGG TCAGGGACAA GCGGCTGCCC 
CCCTGCTCAC TCAACCAAGC CCTCACCTAC TTCCTGGATA TCACCACCCC ACCAACTCAA 
CTGCTGCTCC GAAAGCTGGC CCAGTTGGCC CTGGAAGAGG CCGAGAGGCA GAAGCTAGAA 
GCACTGTGCC AGCCCTCAGA ATATAATAAG TGGAAGTTCA CCAACAGCCC CACGTTCTTG 
GAGGTGCTCG AGGAGTTCCC ATCCCTGCGG GTGTCTGCCA CCTTCCTGCT CTCTCAGCTC 
CCTATTCTGA AACCGCGGTA CTACTCCATC AGCTCCTCCA GGGACCACAC ACCCACGGAG 
GTCCACCTCA CCGTGGCCGT GCTCACCTAC CGCACCCAAG ACGGCCGAGG TCCCCTACAC 
CACGGTGTCT GCAGCACATG GCTTAGCAGC CTGAAGCCCC AAGACTCAGT GCCCTGCTTT 
GTACGAAGTG CCAACGGCTT CCAGCTTCCT GAGGACCCCT CCCATCCTTG CATCCTCATC 
GGGCCTGGCA CGGGCATCGC TCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGATGCT 
GAGCACAAAG GGCTCCAGGG CGGCCGCATG ATCCTGTTGT TTGGGTGCCG GCACCCAGAC 
GAGGACCACC TCTATCAGGA GGAGATGCTG GAGATGGCCC GGAAGGGGGT GCTGCATGGG 
GTGCACACAG CCTATTCTCG CCTACCTGGC CAGCCCAAGG TCTACGTTCA AGACATCCTG 
CAGCAGCAGC TGGCCGGTGA GGTGCTTCGC GTGCTCCACG AGGAGCAAGG CCACCTCTAT 
GTCTGCGGGG ACGTGCGCAT GGCCCGGGAT GTGGCCCACA CCCTGAAGAA GCTGGTGGCT 
GCCAAACTGA GCCTGAGTGA GGAGCAGGTC GAGGACTATT TCTTCCAGCT TAAGAGCCAG 
AAGCGCTATC ATGAAGATAT CTTTGGTGCT GTATTTCCCT ACGAGGTAAA AAAGGATGGG 
GCAGCAGGGC AGCCCAGTAA TATCAGAGCT CCAACAGACC AGAGGAGAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NOS2 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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