POPTR_0013s01380g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

Gene
Clones
gRNAs

Summary

Gene Symbol	POPTR_0013s01380g
Entrez Gene ID	7478921
Full Name	hypothetical protein
Synonyms	POPTRDRAFT_1096093
Gene Type	protein-coding
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024583944.1	XP_024439712.1	phospholipase D zeta 1
XM_002319463.2	XP_002319499.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POPTR_0013s01380g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POPTR_0013s01380g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa28009	XM_002319463.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0013s01380g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPa442171	XM_024583944.1 Latest version!	Populus trichocarpa phospholipase D zeta 1 (LOC7478921), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POPTR_0013s01380g ( XM_002319463.2 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_002319499.1 Populus trichocarpa hypothetical protein (POPTR_0013s01380g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa28009
Clone ID Related Accession (Same CDS sequence)	XM_002319463.2
Accession Version	XM_002319463.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008479). On Dec 16, 2013 this sequence version replaced XM_002319463.1. COMPLETENESS: incomplete on the 5' end.

ATGCATTCTG ATAATGTCCA TGCAAATTTG CATCCATCTT ATTCATTCAG GCAATTCCCT 
GAACCAGGTT GGATTTTTGA TGAATTGCCA AAAGCAACCA TTGTTTCTGT CTCAAGACCT 
GATACTGCTG GAGATTTTAG CCCCATGCTT CTGTCTTACA CCATTGAATT ACAATACAAA 
CAGGCATGCA ACTCTCTCTC TCTCTCTCTC TCCCCCCTTC TCCCTTTCTC TCTTTTGCAA 
GGCAGTCTTC TTTTCAAGTG GCAGTTACTG AAGAAAGCAT CACAAGTTCT CTACTTACAT 
TTTGCTTTGA AGAAGCGTGC ACTTATCGAG GAACTTCATG AAAAACAAGA GCAGGTTAAA 
GAATGGCTCC ACAGTTTAGG AATAGTAGAT CACGCACCAG TCATGCAAGA TGCTGATGAA 
CCGGATGACG GTGCTGTTCC AGTTCATCAT CAAGAAGAAA GTGTTAGAAA CAGAGATGTC 
CCATCCATTG CTGCTTTGTC ATTTCTTCGT CCGGCACTAG GAGGGCAGCA GGGTATTTCA 
GACAGAGCAA AGGTGGCAAT GCAGAATTAT TTGAATCATT TCCTAGGAAA CTTGGATATC 
GTAAATTCGC CAGTGGTCTG CAAATTTTTG GAGGTCTCTA AGTTGTCCTT CTCGAGGGAA 
TATGGTCCAA AGCTGAAAGA AGGTTACATT ATGGCAAAGA ATCTTTCAAA AATCTCCAAG 
GATGATAGTG ATACAACATG TTTTCCCTGC CAATGGTTCG GTTTCTGTGA CAACAACTGG 
CAGAAGGTGT GGGCTGTTTT GAAACCTGGG TTTTTGGCCC TTTTGGAAGA TCCTTTCAAC 
GCAAAAATCA TAGATATTCT TGTTTTTGAT GTTCTGCCAA ACTCAAATGA CAAAGGAGGA 
AACCAAGTAT ATTTAGCAAG CCAGATAAAG GAACGCAATC CTTTGTATTA TGCATTTAAG 
GTTTCTGCCG GAAATCGGAG CATAAACCTG AGAAGCAAAA GTGGTAGTAA AGTTAAAGAA 
TGGATTGCTG CAATCGAAGA TGCTGGTTTG AGGACAAGTG AGGGGTGGTG TCATTCTCAC 
CGCTATGGTT CCTATGCTCC TCCAAGGGGT TTAGCTGAAG ATGGGAGTCA AGCTCAGTGG 
TTTGTGGATG GGCATGCAGC TTTTGAAGCA ATTGCTTCTG CAATAGAGAA TGCAAGATCA 
GAGATATTTA TCACAGGCTG GTGGCTTTGC CCTGAGCTGT ATCTAAGAAG GCCTTTCCAA 
GATCATGCTA GCTCCCGGCT TGATTCCTTG CTAGAAGCTA AAGCTAAGGA AGGGGTTCAG 
ATCTATATCC TTCTCTACAA GGAGGTTTCT ATTGCTTTGA AAATCAACAG TATGTACAGC 
AAGAAAAGGC TTCTTAACAT TCATGAGAAT TTGAGGGTTC TGCGCCATCC TGATCATTTC 
TCAACTGGTG TCTACTCATG GTCACACCAT GAAAAACTAG TGATCATCGA CTACCAGATT 
TGCTTTATTG GAGGATTAGA TTTATGCTTT GGCCGTTATG ACACTATTGA ACATAGAGTA 
GGTGATTGTT CTGCTGACAT ATGGCCTGGA AAGGACTATT ATAATCCAAG AGAATCAGAA 
CCAAATTCTT GGGAAGACGT AATGAAGGAT GAACTGGACC GTAGAAAATA TCCTCGAATG 
CCATGGCATG ATGTCCATTG TTCTCTCTGG GGACCGCCTT GCCGTGACAT TGCTAGGCAC 
TTTGTTCAAC GCTGGAACCA CGCTAAGAGA AGCAAAGCAC CAAATGAGCA AACAATTCCA 
CTACTGATGC CTCGCCACCA CATGGTCCTT CCTCATTACA TGGGAAGAAG TATAGATATT 
GAAAGCAAGA ATGGAGAAGG AAACCAGAAA GACACCAGCA GAATAGATTA CTTTTCCTCA 
GTATCTCCAA TCCGAGATAT CCCTTTGCTT TTACCTCAGG AAGCTGATGC AACAGTTGTT 
AATGGTGTTA ATCACGAGTT AACTGCCAAA AACATGAATA ACGATCGTCT TGATCAATCA 
GCTTGGCACT GCGATAGTTT TTCATTCACT TTGCAGAAAT CCAAGGATGG AAATTTGGCT 
CAAGACACAC CAGTGAAAAA TCCTGTTGAT GAACATGATT TTGTGGATCT TGAAAGCATA 
ATGCAGATTT CAGATCGGTC ATCAGAAACA TCTGAAAAAG ATGTCCCTGA TGTTTCTGCC 
AGCGAATGTG GACAAGTAGG ACCACGTGTC TCATGTCGTT GTCAGGTTAT CAGAAGTGTC 
AGTCAGTGGT CAACAGGAGC CAGTCAGCAT GAAGAAAGCA TCCATAAGGC TTACTGTTCT 
CTCATTGAAA AAGCCGAACA TTTTATCTAC ATAGAGAATC AGTTTTTCAT ATCAGGACTT 
TGTGGAGATG AGATTATCCA GAATCGTGTT TTGGATGCAA TATACAAACG TGTTATTCAA 
GCTTACAAAG AAAATAAGTG CTTTCGAGTC ATCATTGTCA TACCACTCTC ACCAGGTTTC 
CAGGGAGGTG TGGATGATGG TGGTGCAGCA ACTGTTAGAG CCATCATGCA TTGGCAGTAT 
CGGACCATCT CCCGGAAGAA GACTTCAATT CTGTACAATC TTAACACGTT GCTTGGTCCT 
AAAACACATG ATTACATTTC TTTTTGTGGT CTAAGGACGT ATGGTAGACT TTTTGTGGGA 
GGTCCGTTGG TCACAAGTCA GGTTTACGTG CATAGCAAAG TTATGATAGT AGATGACAGG 
ATTGCGTATA TCGGATCATC TAACATCAAT GATAGGAGTT TGCTTGGATC GAGAGACTCT 
GAGATTGGGA TAGTTACTGA AGACAAGGAG TTTGTTGAAT CATCAATGAA CGGGGAGACT 
TGGAAAGCTG GAAAATTTGC TTATAGCCTG CGGCGCTCGC TGTGGTCTGA GCATCTTGGT 
CTTTCTTCAG GAGAGATAGA TAAAATCAGT GATCCTGTAG CTGAGACAAC ATATAGAGAT 
TTATGGTTGG CAACGGCAAA GGAAAATTCA AAGATTTACC AGGATGTCTT TGCTTGTCTT 
CCTAATGATC ATATCCACTC GAGAGCTGCT CTCAGACAAA GCATGAACCA TTGGAAGGAA 
AAACTTGGTC ACACAACCAT TGACTTAGGA ATAGCTCCTG AGAAGATAGA ACGCAATGAA 
AATGGAGAGA TTAAGATGAT GGACCCAATT GAAAGACTGA AATTAGTAAA GGGCCATCTT 
GTTTCCTTTC CATTAGACTT CATGATGTGT CAAGAAGATT TAAGACCAGT ATTCAATGAG 
GGAGAATTTT ATGCATCCCC ACAAGTATTT CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002319499.1
CDS	1..3336
Translation	MHSDNVHANLHPSYSFRQFPEPGWIFDELPKATIVSVSRPDTAGDFSPMLLSYTIELQYKQACNSLSLSLSPLLPFSLLQGSLLFKWQLLKKASQVLYLHFALKKRALIEELHEKQEQVKEWLHSLGIVDHAPVMQDADEPDDGAVPVHHQEESVRNRDVPSIAALSFLRPALGGQQGISDRAKVAMQNYLNHFLGNLDIVNSPVVCKFLEVSKLSFSREYGPKLKEGYIMAKNLSKISKDDSDTTCFPCQWFGFCDNNWQKVWAVLKPGFLALLEDPFNAKIIDILVFDVLPNSNDKGGNQVYLASQIKERNPLYYAFKVSAGNRSINLRSKSGSKVKEWIAAIEDAGLRTSEGWCHSHRYGSYAPPRGLAEDGSQAQWFVDGHAAFEAIASAIENARSEIFITGWWLCPELYLRRPFQDHASSRLDSLLEAKAKEGVQIYILLYKEVSIALKINSMYSKKRLLNIHENLRVLRHPDHFSTGVYSWSHHEKLVIIDYQICFIGGLDLCFGRYDTIEHRVGDCSADIWPGKDYYNPRESEPNSWEDVMKDELDRRKYPRMPWHDVHCSLWGPPCRDIARHFVQRWNHAKRSKAPNEQTIPLLMPRHHMVLPHYMGRSIDIESKNGEGNQKDTSRIDYFSSVSPIRDIPLLLPQEADATVVNGVNHELTAKNMNNDRLDQSAWHCDSFSFTLQKSKDGNLAQDTPVKNPVDEHDFVDLESIMQISDRSSETSEKDVPDVSASECGQVGPRVSCRCQVIRSVSQWSTGASQHEESIHKAYCSLIEKAEHFIYIENQFFISGLCGDEIIQNRVLDAIYKRVIQAYKENKCFRVIIVIPLSPGFQGGVDDGGAATVRAIMHWQYRTISRKKTSILYNLNTLLGPKTHDYISFCGLRTYGRLFVGGPLVTSQVYVHSKVMIVDDRIAYIGSSNINDRSLLGSRDSEIGIVTEDKEFVESSMNGETWKAGKFAYSLRRSLWSEHLGLSSGEIDKISDPVAETTYRDLWLATAKENSKIYQDVFACLPNDHIHSRAALRQSMNHWKEKLGHTTIDLGIAPEKIERNENGEIKMMDPIERLKLVKGHLVSFPLDFMMCQEDLRPVFNEGEFYASPQVFH

Target ORF information:

RefSeq Version	XM_002319463.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0013s01380g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002319463.2

ORF Insert Sequence:

ATGCATTCTG ATAATGTCCA TGCAAATTTG CATCCATCTT ATTCATTCAG GCAATTCCCT 
GAACCAGGTT GGATTTTTGA TGAATTGCCA AAAGCAACCA TTGTTTCTGT CTCAAGACCT 
GATACTGCTG GAGATTTTAG CCCCATGCTT CTGTCTTACA CCATTGAATT ACAATACAAA 
CAGGCATGCA ACTCTCTCTC TCTCTCTCTC TCCCCCCTTC TCCCTTTCTC TCTTTTGCAA 
GGCAGTCTTC TTTTCAAGTG GCAGTTACTG AAGAAAGCAT CACAAGTTCT CTACTTACAT 
TTTGCTTTGA AGAAGCGTGC ACTTATCGAG GAACTTCATG AAAAACAAGA GCAGGTTAAA 
GAATGGCTCC ACAGTTTAGG AATAGTAGAT CACGCACCAG TCATGCAAGA TGCTGATGAA 
CCGGATGACG GTGCTGTTCC AGTTCATCAT CAAGAAGAAA GTGTTAGAAA CAGAGATGTC 
CCATCCATTG CTGCTTTGTC ATTTCTTCGT CCGGCACTAG GAGGGCAGCA GGGTATTTCA 
GACAGAGCAA AGGTGGCAAT GCAGAATTAT TTGAATCATT TCCTAGGAAA CTTGGATATC 
GTAAATTCGC CAGTGGTCTG CAAATTTTTG GAGGTCTCTA AGTTGTCCTT CTCGAGGGAA 
TATGGTCCAA AGCTGAAAGA AGGTTACATT ATGGCAAAGA ATCTTTCAAA AATCTCCAAG 
GATGATAGTG ATACAACATG TTTTCCCTGC CAATGGTTCG GTTTCTGTGA CAACAACTGG 
CAGAAGGTGT GGGCTGTTTT GAAACCTGGG TTTTTGGCCC TTTTGGAAGA TCCTTTCAAC 
GCAAAAATCA TAGATATTCT TGTTTTTGAT GTTCTGCCAA ACTCAAATGA CAAAGGAGGA 
AACCAAGTAT ATTTAGCAAG CCAGATAAAG GAACGCAATC CTTTGTATTA TGCATTTAAG 
GTTTCTGCCG GAAATCGGAG CATAAACCTG AGAAGCAAAA GTGGTAGTAA AGTTAAAGAA 
TGGATTGCTG CAATCGAAGA TGCTGGTTTG AGGACAAGTG AGGGGTGGTG TCATTCTCAC 
CGCTATGGTT CCTATGCTCC TCCAAGGGGT TTAGCTGAAG ATGGGAGTCA AGCTCAGTGG 
TTTGTGGATG GGCATGCAGC TTTTGAAGCA ATTGCTTCTG CAATAGAGAA TGCAAGATCA 
GAGATATTTA TCACAGGCTG GTGGCTTTGC CCTGAGCTGT ATCTAAGAAG GCCTTTCCAA 
GATCATGCTA GCTCCCGGCT TGATTCCTTG CTAGAAGCTA AAGCTAAGGA AGGGGTTCAG 
ATCTATATCC TTCTCTACAA GGAGGTTTCT ATTGCTTTGA AAATCAACAG TATGTACAGC 
AAGAAAAGGC TTCTTAACAT TCATGAGAAT TTGAGGGTTC TGCGCCATCC TGATCATTTC 
TCAACTGGTG TCTACTCATG GTCACACCAT GAAAAACTAG TGATCATCGA CTACCAGATT 
TGCTTTATTG GAGGATTAGA TTTATGCTTT GGCCGTTATG ACACTATTGA ACATAGAGTA 
GGTGATTGTT CTGCTGACAT ATGGCCTGGA AAGGACTATT ATAATCCAAG AGAATCAGAA 
CCAAATTCTT GGGAAGACGT AATGAAGGAT GAACTGGACC GTAGAAAATA TCCTCGAATG 
CCATGGCATG ATGTCCATTG TTCTCTCTGG GGACCGCCTT GCCGTGACAT TGCTAGGCAC 
TTTGTTCAAC GCTGGAACCA CGCTAAGAGA AGCAAAGCAC CAAATGAGCA AACAATTCCA 
CTACTGATGC CTCGCCACCA CATGGTCCTT CCTCATTACA TGGGAAGAAG TATAGATATT 
GAAAGCAAGA ATGGAGAAGG AAACCAGAAA GACACCAGCA GAATAGATTA CTTTTCCTCA 
GTATCTCCAA TCCGAGATAT CCCTTTGCTT TTACCTCAGG AAGCTGATGC AACAGTTGTT 
AATGGTGTTA ATCACGAGTT AACTGCCAAA AACATGAATA ACGATCGTCT TGATCAATCA 
GCTTGGCACT GCGATAGTTT TTCATTCACT TTGCAGAAAT CCAAGGATGG AAATTTGGCT 
CAAGACACAC CAGTGAAAAA TCCTGTTGAT GAACATGATT TTGTGGATCT TGAAAGCATA 
ATGCAGATTT CAGATCGGTC ATCAGAAACA TCTGAAAAAG ATGTCCCTGA TGTTTCTGCC 
AGCGAATGTG GACAAGTAGG ACCACGTGTC TCATGTCGTT GTCAGGTTAT CAGAAGTGTC 
AGTCAGTGGT CAACAGGAGC CAGTCAGCAT GAAGAAAGCA TCCATAAGGC TTACTGTTCT 
CTCATTGAAA AAGCCGAACA TTTTATCTAC ATAGAGAATC AGTTTTTCAT ATCAGGACTT 
TGTGGAGATG AGATTATCCA GAATCGTGTT TTGGATGCAA TATACAAACG TGTTATTCAA 
GCTTACAAAG AAAATAAGTG CTTTCGAGTC ATCATTGTCA TACCACTCTC ACCAGGTTTC 
CAGGGAGGTG TGGATGATGG TGGTGCAGCA ACTGTTAGAG CCATCATGCA TTGGCAGTAT 
CGGACCATCT CCCGGAAGAA GACTTCAATT CTGTACAATC TTAACACGTT GCTTGGTCCT 
AAAACACATG ATTACATTTC TTTTTGTGGT CTAAGGACGT ATGGTAGACT TTTTGTGGGA 
GGTCCGTTGG TCACAAGTCA GGTTTACGTG CATAGCAAAG TTATGATAGT AGATGACAGG 
ATTGCGTATA TCGGATCATC TAACATCAAT GATAGGAGTT TGCTTGGATC GAGAGACTCT 
GAGATTGGGA TAGTTACTGA AGACAAGGAG TTTGTTGAAT CATCAATGAA CGGGGAGACT 
TGGAAAGCTG GAAAATTTGC TTATAGCCTG CGGCGCTCGC TGTGGTCTGA GCATCTTGGT 
CTTTCTTCAG GAGAGATAGA TAAAATCAGT GATCCTGTAG CTGAGACAAC ATATAGAGAT 
TTATGGTTGG CAACGGCAAA GGAAAATTCA AAGATTTACC AGGATGTCTT TGCTTGTCTT 
CCTAATGATC ATATCCACTC GAGAGCTGCT CTCAGACAAA GCATGAACCA TTGGAAGGAA 
AAACTTGGTC ACACAACCAT TGACTTAGGA ATAGCTCCTG AGAAGATAGA ACGCAATGAA 
AATGGAGAGA TTAAGATGAT GGACCCAATT GAAAGACTGA AATTAGTAAA GGGCCATCTT 
GTTTCCTTTC CATTAGACTT CATGATGTGT CAAGAAGATT TAAGACCAGT ATTCAATGAG 
GGAGAATTTT ATGCATCCCC ACAAGTATTT CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0013s01380g ( XM_024583944.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024439712.1 Populus trichocarpa phospholipase D zeta 1 (LOC7478921), mRNA.

Price & Availability↑

CloneID	OPa442171
Clone ID Related Accession (Same CDS sequence)	XM_024583944.1
Accession Version	XM_024583944.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	phospholipase D zeta 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037297.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAA CAACAACAGA GCCACTGTTA GCGGCAGGGA CTATGCATTC TGATAATGTC 
CATGCAAATT TGCATCCATC TTATTCATTC AGGCAATTCC CTGAACCAGG TTGGATTTTT 
GATGAATTGC CAAAAGCAAC CATTGTTTCT GTCTCAAGAC CTGATACTGC TGGAGATTTT 
AGCCCCATGC TTCTGTCTTA CACCATTGAA TTACAATACA AACAGTTCAA GTGGCAGTTA 
CTGAAGAAAG CATCACAAGT TCTCTACTTA CATTTTGCTT TGAAGAAGCG TGCACTTATC 
GAGGAACTTC ATGAAAAACA AGAGCAGGTT AAAGAATGGC TCCACAGTTT AGGAATAGTA 
GATCACGCAC CAGTCATGCA AGATGCTGAT GAACCGGATG ACGGTGCTGT TCCAGTTCAT 
CATCAAGAAG AAAGTGTTAG AAACAGAGAT GTCCCATCCA TTGCTGCTTT GTCATTTCTT 
CGTCCGGCAC TAGGAGGGCA GCAGGGTATT TCAGACAGAG CAAAGGTGGC AATGCAGAAT 
TATTTGAATC ATTTCCTAGG AAACTTGGAT ATCGTAAATT CGCCAGTGGT CTGCAAATTT 
TTGGAGGTCT CTAAGTTGTC CTTCTCGAGG GAATATGGTC CAAAGCTGAA AGAAGGTTAC 
ATTATGGCAA AGAATCTTTC AAAAATCTCC AAGGATGATA GTGATACAAC ATGTTTTCCC 
TGCCAATGGT TCGGTTTCTG TGACAACAAC TGGCAGAAGG TGTGGGCTGT TTTGAAACCT 
GGGTTTTTGG CCCTTTTGGA AGATCCTTTC AACGCAAAAA TCATAGATAT TCTTGTTTTT 
GATGTTCTGC CAAACTCAAA TGACAAAGGA GGAAACCAAG TATATTTAGC AAGCCAGATA 
AAGGAACGCA ATCCTTTGTA TTATGCATTT AAGGTTTCTG CCGGAAATCG GAGCATAAAC 
CTGAGAAGCA AAAGTGGTAG TAAAGTTAAA GAATGGATTG CTGCAATCGA AGATGCTGGT 
TTGAGGACAA GTGAGGGGTG GTGTCATTCT CACCGCTATG GTTCCTATGC TCCTCCAAGG 
GGTTTAGCTG AAGATGGGAG TCAAGCTCAG TGGTTTGTGG ATGGGCATGC AGCTTTTGAA 
GCAATTGCTT CTGCAATAGA GAATGCAAGA TCAGAGATAT TTATCACAGG CTGGTGGCTT 
TGCCCTGAGC TGTATCTAAG AAGGCCTTTC CAAGATCATG CTAGCTCCCG GCTTGATTCC 
TTGCTAGAAG CTAAAGCTAA GGAAGGGGTT CAGATCTATA TCCTTCTCTA CAAGGAGGTT 
TCTATTGCTT TGAAAATCAA CAGTATGTAC AGCAAGAAAA GGCTTCTTAA CATTCATGAG 
AATTTGAGGG TTCTGCGCCA TCCTGATCAT TTCTCAACTG GTGTCTACTC ATGGTCACAC 
CATGAAAAAC TAGTGATCAT CGACTACCAG ATTTGCTTTA TTGGAGGATT AGATTTATGC 
TTTGGCCGTT ATGACACTAT TGAACATAGA GTAGGTGATT GTTCTGCTGA CATATGGCCT 
GGAAAGGACT ATTATAATCC AAGAGAATCA GAACCAAATT CTTGGGAAGA CGTAATGAAG 
GATGAACTGG ACCGTAGAAA ATATCCTCGA ATGCCATGGC ATGATGTCCA TTGTTCTCTC 
TGGGGACCGC CTTGCCGTGA CATTGCTAGG CACTTTGTTC AACGCTGGAA CCACGCTAAG 
AGAAGCAAAG CACCAAATGA GCAAACAATT CCACTACTGA TGCCTCGCCA CCACATGGTC 
CTTCCTCATT ACATGGGAAG AAGTATAGAT ATTGAAAGCA AGAATGGAGA AGGAAACCAG 
AAAGACACCA GCAGAATAGA TTACTTTTCC TCAGTATCTC CAATCCGAGA TATCCCTTTG 
CTTTTACCTC AGGAAGCTGA TGCAACAGTT GTTAATGGTG TTAATCACGA GTTAACTGCC 
AAAAACATGA ATAACGATCG TCTTGATCAA TCAGCTTGGC ACTGCGATAG TTTTTCATTC 
ACTTTGCAGA AATCCAAGGA TGGAAATTTG GCTCAAGACA CACCAGTGAA AAATCCTGTT 
GATGAACATG ATTTTGTGGA TCTTGAAAGC ATAATGCAGA TTTCAGATCG GTCATCAGAA 
ACATCTGAAA AAGATGTCCC TGATGTTTCT GCCAGCGAAT GTGGACAAGT AGGACCACGT 
GTCTCATGTC GTTGTCAGGT TATCAGAAGT GTCAGTCAGT GGTCAACAGG AGCCAGTCAG 
CATGAAGAAA GCATCCATAA GGCTTACTGT TCTCTCATTG AAAAAGCCGA ACATTTTATC 
TACATAGAGA ATCAGTTTTT CATATCAGGA CTTTGTGGAG ATGAGATTAT CCAGAATCGT 
GTTTTGGATG CAATATACAA ACGTGTTATT CAAGCTTACA AAGAAAATAA GTGCTTTCGA 
GTCATCATTG TCATACCACT CTCACCAGGT TTCCAGGGAG GTGTGGATGA TGGTGGTGCA 
GCAACTGTTA GAGCCATCAT GCATTGGCAG TATCGGACCA TCTCCCGGAA GAAGACTTCA 
ATTCTGTACA ATCTTAACAC GTTGCTTGGT CCTAAAACAC ATGATTACAT TTCTTTTTGT 
GGTCTAAGGA CGTATGGTAG ACTTTTTGTG GGAGGTCCGT TGGTCACAAG TCAGGTTTAC 
GTGCATAGCA AAGTTATGAT AGTAGATGAC AGGATTGCGT ATATCGGATC ATCTAACATC 
AATGATAGGA GTTTGCTTGG ATCGAGAGAC TCTGAGATTG GGATAGTTAC TGAAGACAAG 
GAGTTTGTTG AATCATCAAT GAACGGGGAG ACTTGGAAAG CTGGAAAATT TGCTTATAGC 
CTGCGGCGCT CGCTGTGGTC TGAGCATCTT GGTCTTTCTT CAGGAGAGAT AGATAAAATC 
AGTGATCCTG TAGCTGAGAC AACATATAGA GATTTATGGT TGGCAACGGC AAAGGAAAAT 
TCAAAGATTT ACCAGGATGT CTTTGCTTGT CTTCCTAATG ATCATATCCA CTCGAGAGCT 
GCTCTCAGAC AAAGCATGAA CCATTGGAAG GAAAAACTTG GTCACACAAC CATTGACTTA 
GGAATAGCTC CTGAGAAGAT AGAACGCAAT GAAAATGGAG AGATTAAGAT GATGGACCCA 
ATTGAAAGAC TGAAATTAGT AAAGGGCCAT CTTGTTTCCT TTCCATTAGA CTTCATGATG 
TGTCAAGAAG ATTTAAGACC AGTATTCAAT GAGGGAGAAT TTTATGCATC CCCACAAGTA 
TTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024439712.1
CDS	493..3801
Translation	MATTTTEPLLAAGTMHSDNVHANLHPSYSFRQFPEPGWIFDELPKATIVSVSRPDTAGDFSPMLLSYTIELQYKQFKWQLLKKASQVLYLHFALKKRALIEELHEKQEQVKEWLHSLGIVDHAPVMQDADEPDDGAVPVHHQEESVRNRDVPSIAALSFLRPALGGQQGISDRAKVAMQNYLNHFLGNLDIVNSPVVCKFLEVSKLSFSREYGPKLKEGYIMAKNLSKISKDDSDTTCFPCQWFGFCDNNWQKVWAVLKPGFLALLEDPFNAKIIDILVFDVLPNSNDKGGNQVYLASQIKERNPLYYAFKVSAGNRSINLRSKSGSKVKEWIAAIEDAGLRTSEGWCHSHRYGSYAPPRGLAEDGSQAQWFVDGHAAFEAIASAIENARSEIFITGWWLCPELYLRRPFQDHASSRLDSLLEAKAKEGVQIYILLYKEVSIALKINSMYSKKRLLNIHENLRVLRHPDHFSTGVYSWSHHEKLVIIDYQICFIGGLDLCFGRYDTIEHRVGDCSADIWPGKDYYNPRESEPNSWEDVMKDELDRRKYPRMPWHDVHCSLWGPPCRDIARHFVQRWNHAKRSKAPNEQTIPLLMPRHHMVLPHYMGRSIDIESKNGEGNQKDTSRIDYFSSVSPIRDIPLLLPQEADATVVNGVNHELTAKNMNNDRLDQSAWHCDSFSFTLQKSKDGNLAQDTPVKNPVDEHDFVDLESIMQISDRSSETSEKDVPDVSASECGQVGPRVSCRCQVIRSVSQWSTGASQHEESIHKAYCSLIEKAEHFIYIENQFFISGLCGDEIIQNRVLDAIYKRVIQAYKENKCFRVIIVIPLSPGFQGGVDDGGAATVRAIMHWQYRTISRKKTSILYNLNTLLGPKTHDYISFCGLRTYGRLFVGGPLVTSQVYVHSKVMIVDDRIAYIGSSNINDRSLLGSRDSEIGIVTEDKEFVESSMNGETWKAGKFAYSLRRSLWSEHLGLSSGEIDKISDPVAETTYRDLWLATAKENSKIYQDVFACLPNDHIHSRAALRQSMNHWKEKLGHTTIDLGIAPEKIERNENGEIKMMDPIERLKLVKGHLVSFPLDFMMCQEDLRPVFNEGEFYASPQVFH

Target ORF information:

RefSeq Version	XM_024583944.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa phospholipase D zeta 1 (LOC7478921), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024583944.1

ORF Insert Sequence:

ATGGCAACAA CAACAACAGA GCCACTGTTA GCGGCAGGGA CTATGCATTC TGATAATGTC 
CATGCAAATT TGCATCCATC TTATTCATTC AGGCAATTCC CTGAACCAGG TTGGATTTTT 
GATGAATTGC CAAAAGCAAC CATTGTTTCT GTCTCAAGAC CTGATACTGC TGGAGATTTT 
AGCCCCATGC TTCTGTCTTA CACCATTGAA TTACAATACA AACAGTTCAA GTGGCAGTTA 
CTGAAGAAAG CATCACAAGT TCTCTACTTA CATTTTGCTT TGAAGAAGCG TGCACTTATC 
GAGGAACTTC ATGAAAAACA AGAGCAGGTT AAAGAATGGC TCCACAGTTT AGGAATAGTA 
GATCACGCAC CAGTCATGCA AGATGCTGAT GAACCGGATG ACGGTGCTGT TCCAGTTCAT 
CATCAAGAAG AAAGTGTTAG AAACAGAGAT GTCCCATCCA TTGCTGCTTT GTCATTTCTT 
CGTCCGGCAC TAGGAGGGCA GCAGGGTATT TCAGACAGAG CAAAGGTGGC AATGCAGAAT 
TATTTGAATC ATTTCCTAGG AAACTTGGAT ATCGTAAATT CGCCAGTGGT CTGCAAATTT 
TTGGAGGTCT CTAAGTTGTC CTTCTCGAGG GAATATGGTC CAAAGCTGAA AGAAGGTTAC 
ATTATGGCAA AGAATCTTTC AAAAATCTCC AAGGATGATA GTGATACAAC ATGTTTTCCC 
TGCCAATGGT TCGGTTTCTG TGACAACAAC TGGCAGAAGG TGTGGGCTGT TTTGAAACCT 
GGGTTTTTGG CCCTTTTGGA AGATCCTTTC AACGCAAAAA TCATAGATAT TCTTGTTTTT 
GATGTTCTGC CAAACTCAAA TGACAAAGGA GGAAACCAAG TATATTTAGC AAGCCAGATA 
AAGGAACGCA ATCCTTTGTA TTATGCATTT AAGGTTTCTG CCGGAAATCG GAGCATAAAC 
CTGAGAAGCA AAAGTGGTAG TAAAGTTAAA GAATGGATTG CTGCAATCGA AGATGCTGGT 
TTGAGGACAA GTGAGGGGTG GTGTCATTCT CACCGCTATG GTTCCTATGC TCCTCCAAGG 
GGTTTAGCTG AAGATGGGAG TCAAGCTCAG TGGTTTGTGG ATGGGCATGC AGCTTTTGAA 
GCAATTGCTT CTGCAATAGA GAATGCAAGA TCAGAGATAT TTATCACAGG CTGGTGGCTT 
TGCCCTGAGC TGTATCTAAG AAGGCCTTTC CAAGATCATG CTAGCTCCCG GCTTGATTCC 
TTGCTAGAAG CTAAAGCTAA GGAAGGGGTT CAGATCTATA TCCTTCTCTA CAAGGAGGTT 
TCTATTGCTT TGAAAATCAA CAGTATGTAC AGCAAGAAAA GGCTTCTTAA CATTCATGAG 
AATTTGAGGG TTCTGCGCCA TCCTGATCAT TTCTCAACTG GTGTCTACTC ATGGTCACAC 
CATGAAAAAC TAGTGATCAT CGACTACCAG ATTTGCTTTA TTGGAGGATT AGATTTATGC 
TTTGGCCGTT ATGACACTAT TGAACATAGA GTAGGTGATT GTTCTGCTGA CATATGGCCT 
GGAAAGGACT ATTATAATCC AAGAGAATCA GAACCAAATT CTTGGGAAGA CGTAATGAAG 
GATGAACTGG ACCGTAGAAA ATATCCTCGA ATGCCATGGC ATGATGTCCA TTGTTCTCTC 
TGGGGACCGC CTTGCCGTGA CATTGCTAGG CACTTTGTTC AACGCTGGAA CCACGCTAAG 
AGAAGCAAAG CACCAAATGA GCAAACAATT CCACTACTGA TGCCTCGCCA CCACATGGTC 
CTTCCTCATT ACATGGGAAG AAGTATAGAT ATTGAAAGCA AGAATGGAGA AGGAAACCAG 
AAAGACACCA GCAGAATAGA TTACTTTTCC TCAGTATCTC CAATCCGAGA TATCCCTTTG 
CTTTTACCTC AGGAAGCTGA TGCAACAGTT GTTAATGGTG TTAATCACGA GTTAACTGCC 
AAAAACATGA ATAACGATCG TCTTGATCAA TCAGCTTGGC ACTGCGATAG TTTTTCATTC 
ACTTTGCAGA AATCCAAGGA TGGAAATTTG GCTCAAGACA CACCAGTGAA AAATCCTGTT 
GATGAACATG ATTTTGTGGA TCTTGAAAGC ATAATGCAGA TTTCAGATCG GTCATCAGAA 
ACATCTGAAA AAGATGTCCC TGATGTTTCT GCCAGCGAAT GTGGACAAGT AGGACCACGT 
GTCTCATGTC GTTGTCAGGT TATCAGAAGT GTCAGTCAGT GGTCAACAGG AGCCAGTCAG 
CATGAAGAAA GCATCCATAA GGCTTACTGT TCTCTCATTG AAAAAGCCGA ACATTTTATC 
TACATAGAGA ATCAGTTTTT CATATCAGGA CTTTGTGGAG ATGAGATTAT CCAGAATCGT 
GTTTTGGATG CAATATACAA ACGTGTTATT CAAGCTTACA AAGAAAATAA GTGCTTTCGA 
GTCATCATTG TCATACCACT CTCACCAGGT TTCCAGGGAG GTGTGGATGA TGGTGGTGCA 
GCAACTGTTA GAGCCATCAT GCATTGGCAG TATCGGACCA TCTCCCGGAA GAAGACTTCA 
ATTCTGTACA ATCTTAACAC GTTGCTTGGT CCTAAAACAC ATGATTACAT TTCTTTTTGT 
GGTCTAAGGA CGTATGGTAG ACTTTTTGTG GGAGGTCCGT TGGTCACAAG TCAGGTTTAC 
GTGCATAGCA AAGTTATGAT AGTAGATGAC AGGATTGCGT ATATCGGATC ATCTAACATC 
AATGATAGGA GTTTGCTTGG ATCGAGAGAC TCTGAGATTG GGATAGTTAC TGAAGACAAG 
GAGTTTGTTG AATCATCAAT GAACGGGGAG ACTTGGAAAG CTGGAAAATT TGCTTATAGC 
CTGCGGCGCT CGCTGTGGTC TGAGCATCTT GGTCTTTCTT CAGGAGAGAT AGATAAAATC 
AGTGATCCTG TAGCTGAGAC AACATATAGA GATTTATGGT TGGCAACGGC AAAGGAAAAT 
TCAAAGATTT ACCAGGATGT CTTTGCTTGT CTTCCTAATG ATCATATCCA CTCGAGAGCT 
GCTCTCAGAC AAAGCATGAA CCATTGGAAG GAAAAACTTG GTCACACAAC CATTGACTTA 
GGAATAGCTC CTGAGAAGAT AGAACGCAAT GAAAATGGAG AGATTAAGAT GATGGACCCA 
ATTGAAAGAC TGAAATTAGT AAAGGGCCAT CTTGTTTCCT TTCCATTAGA CTTCATGATG 
TGTCAAGAAG ATTTAAGACC AGTATTCAAT GAGGGAGAAT TTTATGCATC CCCACAAGTA 
TTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,Déjardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasjärvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Leplé JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouzé P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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POPTR_0013s01380g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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